Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| purification of cauliflower mosaic virus. | 1960 | 13814596 | |
| purification and properties of cauliflower mosaic virus. | 1960 | 14433226 | |
| dna in cauliflower mosaic virus. | 1968 | 5669985 | |
| conformation changes in cauliflower mosaic virus. | 1969 | 5358075 | |
| thymidine-3h uptake by x-bodies associated with cauliflower mosaic virus infection. | 1969 | 5777571 | |
| double-stranded dna from cauliflower mosaic virus. | 1970 | 4316313 | |
| light and electron microscopy of the intracellular inclusions of cauliflower mosaic virus. | 1971 | 4108668 | |
| the double-stranded dna of cauliflower mosaic virus. | 1971 | 5566091 | |
| inhibition of cauliflower mosaic virus multiplication by actinomycin d. | 1971 | 5119661 | |
| structural protein of cauliflower mosaic virus. | 1972 | 5017154 | |
| some ultrastructural and cytochemical observations on chinese cabbage leaves infected with cauliflower mosaic virus. | 1972 | 4111055 | |
| stepwise degradation of cauliflower mosaic virus by pronase. | 1972 | 5058330 | |
| a quantitative autoradiographic study of intracellular sites for replication of cauliflower mosaic virus. | 1973 | 4706708 | |
| acquisition factor required for aphid transmission of purified cauliflower mosaic virus. | 1974 | 4841178 | |
| cauliflower mosaic virus structural proteins. | 1974 | 4850917 | |
| purification and characterization of dna-dependent rna polymerases from cauliflower nuclei. | dna-dependent rna polymerases were solubilized from nuclei of cauliflower inflorescences and purified by agarose a-1.5m, deae-cellulose, deae-sephadex, and phosphocellulose chromatography and sucrose density gradient centrifugation. rna polymerases i + iii were separated from ii by deae-cellulose chromatography. subsequent chromatography on deae-sephadex resolved rna polymerase i from iii. rna polymerases i and ii were further purified to high specific activity by phosphocellulose chromatography ... | 1976 | 16659696 |
| the coat proteins of cauliflower mosaic virus. | 1976 | 1258379 | |
| [virus infestation of garden radish (raphanus sativus l. var. sativus) (author's transl)]. | in july 1974 a mosaic disease was stated in seed plants of garden radish near aschersleben which spread more and more within some weeks and eventually infected over 40 per cent of the stand. investigations in comparison with healthy seed plants demonstrated the following average losses induced by the disease: number of pods 51 per cent, seed weight 67 per cent, number of seeds per plant 53 per cent, weight of 1,000 seeds 33 per cent. nearly unaffected were: number of seeds per pod, percentage of ... | 1976 | 1037175 |
| photoreactivation of dna-containing cauliflower mosaic virus and tobacco mosaic virus rna on datura. | 1977 | 905348 | |
| cloning of cauliflower mosaic virus (clmv) dna in escherichia coli. | a plasmid containing cauliflower mosaic virus dna can be faithfully cloned in escherichia coli, but proved to be noninfective in test plants. | 1977 | 322284 |
| the structure of cauliflower mosaic virus. i. a restriction endonuclease map of cauliflower mosaic virus dna. | 1977 | 329557 | |
| protein expression in e. coli minicells by recombinant plasmids. | the polypeptides synthesized in e. coli minicells from recombinant plasmids containing dna fragments from cauliflower mosaic virus, drosophila melanogaster, and mouse mitochondria were examined. molecularly cloned fragments of cauliflower mosaic virus dna directed the synthesis of high levels of three polypeptides, which were synthesized entirely from within the cloned virus dna fragments independent of their insertion into the plasmid vehicles. several fragments of d. melanogaster dna were capa ... | 1977 | 403011 |
| the structure of cauliflower mosaic virus genome. | 1977 | 559371 | |
| physical map of dna from a new cauliflower mosaic virus strain. | the restriction enzymes alui, bamhi, bglii, ecori, hindiii, and sali have been used to characterize and map a new cauliflower mosaic virus strain (cabb-s). these fragments have been ordered by examining their overlapping regions after double enzymatic digestion. the single sali cleavage site was chosen as the point of origin. we compare this strain with those already described. | 1978 | 744486 |
| studies on the single-stranded discontinuities of the cauliflower mosaic virus genome. | the cauliflower mosaic virus (camv) genome is a double-stranded dna molecule of about 5 million daltons. native dna molecules appear heterogeneous when analysed by gel electrophoresis. we have examined the nature of this apparent heterogeneity. besides, this genome is shown here to contain three single-stranded breaks, as revealed by different denaturation experiments: heating at 75 degrees c, treatment with naoh or dimethyl sulfoxide (dmso). labelling with terminal transferase proves that the 3 ... | 1978 | 693323 |
| structure of the cauliflower mosaic virus genome. ii. variation in dna structure and sequence between isolates. | 1978 | 664243 | |
| replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplasts. | 1978 | 664242 | |
| a restriction map of cauliflower mosaic virus dna (strain pv 147). mapping of the cleavage sites of hhai, saci, avai, pvuii, psti, xbai, ecori, bg/ii, hincii, hpaii and hindii + iii. | the virion-extracted dna (mr5 x 10(6)) of cauliflower mosaic virus (camv) has three single-stranded interruptions. the mapping of this dna using eleven restriction endonucleases (hhai, saci, avai, pvuii, psti, xbai, ecori, bg/ii, hincii, hpaii and hindii + iii) is reported here. the existence of the three single-stranded breaks complicates the identification and the molecular weight determination of fragments produced by hpaii, hindiii and hindii + iii. indeed the electrophoretic mobility of som ... | 1979 | 488094 |
| the polarity of the cauliflower mosaic virus genome. | we have demonstrated that the 5' termini of the dnas of cauliflower mosaic virus (camv) isolates cm4-184 and cabb b-ji have free hydroxyl groups accessible for phosphorylation using polynucleotide kinase. this has enabled us, using the cabb b-ji isolate, to label and identify the 5' nucleotides. digestion of 5' terminally labelled dna with ecori restriction endonuclease revealed that only three of the eleven single-stranded ecori fragments were labelled, those situated adjacent to the three know ... | 1979 | 503842 |
| the structure of cauliflower mosaic virus: a neutron diffraction study. | neutron scattering has been used to determine the organization of cauliflower mosaic virus. the virus can be represented by a model that consists essentially of a protein shell surrounding and in direct contact with a shell of dna. protein seems to be present at a low concentration directly beneath the dna shell, and the center of the virion seems to be free of dna and protein. | 1979 | 18631597 |
| transcription in vitro of cauliflower mosaic virus dna by rna polymerase i, ii, and iii purified from wheat embryos. | 1979 | 486178 | |
| the structure of cauliflower mosaic virus. ii. identity and location of the viral polypeptides. | 1979 | 433152 | |
| asymmetric transcription of cauliflower mosaic virus genome by the escherichia coli rna polymerase in vitro. | 1980 | 6250888 | |
| infectivities of native and cloned dna of cauliflower mosaic virus. | infectivity assays on turnips reveal that (i) cauliflower mosaic virus (camv) dna, whether circular or linear, is as infectious as the complete virus; (ii) linear dna obtained with restriction enzymes from the native camv dna has the same specific infectivity as when first cloned in plasmid (pbr322) or bacteriophage (lambda gtwes) vectors and then restricted at the cloning site; (iii) in all cases studied mosaic symptoms are accompanied by virus production. dna isolated from these viruses is aga ... | 1980 | 6260583 |
| restriction map of native and cloned cauliflower mosaic virus dna. | cloned camv dna replicates faithfully in escherichia coli, since the restriction map of the cloned dna can be superimposed over that of the native viral dna. however, some short fragments were difficult to detect in the restricted native viral dna, whereas they formed clear bands when derived from cauliflower mosaic virus (camv) dna clones propagated in the e. coli host. apparently, the small fragments that carry variable-length single-stranded gaps present only in native viral dna, give rise to ... | 1980 | 7002732 |
| the effect of limited proteolysis on the antigenic stability of cauliflower mosaic virus. | serological differences between several isolates of cauliflower mosaic virus (camv) were shown by double antibody enzyme immunoassays and serum cross-absorption. relationships established in this way were not consistent, and as a possible reason, the effect of naturally occurring proteolysis of the camv capsid polypeptide on the serological reactivity of the virus was investigated. changes in serological reactivity due to proteolysis were of a similar magnitude as the differences in reactivity b ... | 1980 | 18631811 |
| phosphorylated proteins in cauliflower mosaic virus. | autoradiography of dodecyl sulfate-impregnated polyacrylamide gels after electrophoresis of the proteins from 32p-labeled cauliflower mosaic virus (camv) showed that two of the structural proteins were phosphorylated. these two proteins of 44 and 58 kilodaltons (kd) occur as minor components with some strains of the virus, but with other virus strains under particular conditions the 44-kd protein appears as a major component of the virion. the site of phosphorylation was found to be phosphoserin ... | 1980 | 18631810 |
| transcription of the cauliflower mosaic virus genome in isolated nuclei from turnip leaves. | nuclei isolated from turnip (brassica rapa l. c.v. just right) leaves infected with cauliflower mosaic virus synthesize rna in vitro which hybridizes to purified cauliflower mosaic virus dna. nuclei isolated from uninfected leaves do not produce these viral transcripts in vitro. viral-specific transcription in isolated nuclei is catalyzed by endogenous dna-dependent rna polymerase 11 based on sensitivity to alpha-amanitin and ionic strength optima. only one strand of the viral genome is transcri ... | 1980 | 18631807 |
| a procedure for rapid isolation and analysis of cauliflower mosaic virus dna. | a simple and rapid procedure has been developed for the small-scale isolation of crude preparations of cauliflower mosaic virus dna. the preparations are relatively free of contaminating cellular dna and are sufficiently pure to be digested with restriction enzymes. the method enables plants infected with single-lesion isolates of mutagenized viral dna to be rapidly screened for particular restriction endonuclease sites. | 1980 | 18631716 |
| polypeptides associated with inclusion bodies from leaves of turnip infected with cauliflower mosaic virus. | inclusion bodies, the major intracellular site of accumulation of cauliflower mosaic virus (camv), have been partially purified by centrifugation onto a saturated sucrose cushion, followed by detergent treatment to lyse chloroplasts, then centrifugation on a saturated sucrose cushion again. the resulting preparation was enriched for inclusion bodies as judged by light and electron microscopic observation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the polypeptides in the ... | 1980 | 18631681 |
| nucleotide sequence of cauliflower mosaic virus dna. | the complete nucleotide sequence (8024 nucleotides) of the circular double-stranded dna of cauliflower mosaic virus has been established. the dna molecule is known to possess three discrete single-stranded discontinuities, often referred to as "gaps," two in one strand and one in the other. the sequence data indicate that gap 1, the single discontinuity in the alpha strand, corresponds to the absence of no more than one or two nucleotides with respect to the complementary beta strand. the two di ... | 1980 | 7407912 |
| cloned cauliflower mosaic virus dna infects turnips (brassica rapa). | cauliflower mosaic virus dna cloned in the sal i site of bacterial plasmid pbr322 infects turnip plants. the cloned viral dna must be excised from the recombinant plasmid to infect, but need not be circularized and ligated in vitro. the cloned viral dna lacks site-specific single-strand breaks found in dna obtained directly from the virus. however, these breaks are reintroduced into the viral genome during multiplication of the virus in the plant host. | 1980 | 17830812 |
| restriction enzyme cleavage maps of the dna of two cauliflower mosaic virus isolates. | to provide a physical basis for the functional mapping of the cauliflower mosaic virus (camv) genome, restriction enzyme cleavage maps of the dna of camv isolates ny8153 and cm4-184-os (a variant of cm4-184) were constructed. cm4-184-os dna contained five ecori, eight hindiii six bgliii, and two bamhi cleavage sites, while ny8153 dna contained one additional hindiii site and one fewer ecori site. fragments were ordered by examination of multienzyme and partial digests. the resulting maps reveale ... | 1980 | 18631651 |
| isolation and properties of the inclusion bodies of cauliflower mosaic virus. | in an attempt to isolate new gene products of cauliflower mosaic virus a procedure was developed for isolation of the cytoplasmic inclusion bodies induced by the virus. two types of inclusions, the relative ratios of which vary with virus strain, were obtained. one type was the native, vacuolated inclusion encountered in sections of tissue observed with the electron microscope. it contains virus particles. a second, granular type of inclusion body is devoid of virus. it consists wholly of electr ... | 1980 | 18631648 |
| comparative cytology of nine isolates of cauliflower mosaic virus. | the cytology of nine isolates of cauliflower mosaic virus was compared by light and electron microscopy. differences noted among the isolates were: the size of cytoplasmic inclusion bodies, frequency of virions occurring free in the cytoplasm as opposed to those in inclusions, the ratio of virions to the amount of matrix protein in inclusions, and intraplastidial inclusions induced by one of the isolates. it was concluded that these four cytological features are expressions of the viral genome r ... | 1980 | 18631647 |
| the identification, mapping, and characterization of mrna for p66, a cauliflower mosaic virus-coded protein. | turnip leaves infected with cauliflower mosaic virus accumulate variable amounts of a nonvirion protein of 66,000 mw. tryptic fingerprint analysis showed that this protein is probably not related to the major virus coat protein (37,000 mw). rna extracted from infected leaves directs the synthesis of a 66,000 mw protein, p66, in a wheat germ protein-synthesizing system. p66 synthesis in vitro could be arrested by prior hybridization of infected leaf rna to viral dna restriction fragments indicati ... | 1980 | 18631646 |
| structure of the cauliflower mosaic virus genome. iii. restriction endonuclease mapping of thirty-three isolates. | the sites of various restriction endonucleases were mapped on the dna of cauliflower mosaic virus isolate cabb b-ji.fspai,hgiai,hhai, andxhoi each cut at one site,psti andpvuii each at two sites,bglii at five sites, andhindiii at nine sites;sacp,smai, andxbai did not cut this dna. these sites and those ofbamhi,ecori, andsalgi were compared with the sites of these enzymes on the dnas of 32 other camv isolates. considerable variations were found both in numbers and map positions of the sites of th ... | 1980 | 18631629 |
| the complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by m13mp7 shotgun sequencing. | we have determined the complete primary structure (8031 base pairs) of an infectious clone of cauliflower mosaic virus strain cm1841. the sequence was obtained using the strategy of cloning shotgun restriction fragments in the sequencing vector m13mp7. comparison of the cm1841 sequence with that published for another camv strain (strasbourg) reveals 4.4% changes, mostly nucleotide substitutions with a few small insertions and deletions. the six open reading frames in the sequence of the strasbou ... | 1981 | 6269062 |
| isolation and mapping of small cauliflower mosaic virus dna fragments active as promoters in escherichia coli. | small ecori(*) fragments of cauliflower mosiac virus dna (strain cm4-184), which act as promoters for the tetracycline resistance gene on the promoter probe plasmid pbrh4 in escherichia coli, have been isolated and mapped on the viral genome. two regions of the viral genome contain dna sequences with promoter activity in e. coli. two independent cloned fragments from one region direct a high level of tetracycline resistance (up to 38 mug of tetracycline per ml). two independent fragments from th ... | 1981 | 16789206 |
| transcription of cauliflower mosaic virus dna. detection of transcripts, properties, and location of the gene encoding the virus inclusion body protein. | we have detected several cauliflower mosaic virus transcripts in infected turnip leaves by northern-blot hybridization. these rnas ranged in size from 0.9 to about 8 kb. two species, a heterogeneous 7-to 8-kb rna and a 2.3-kb rna, accumulated radioactivity when camv-infected leaves were labeled with [32p]orthophosphate 20 days postinoculation. an abundant 62,000 mw polypeptide was synthesized in a rabbit reticulocyte lysate programmed with rna from infected plants, but this polypeptide was absen ... | 1981 | 18635057 |
| characterisation of cauliflower mosaic virus dna sequences which encode major polyadenylated transcripts. | cauliflower mosaic virus dna sequences which encode two major polyadenylated rna species, the 1.9 kb messenger rna for the 62,000 mw virus inclusion body polypeptide and 35s rna, were mapped using the nuclease 51 procedure. the 1.9 kb rna has an eleven nucleotide leader sequence transcribed from alpha-strand dna located at co-ordinate 0.72 m.u. (map units), immediately upstream of the aug initiation codon of reading frame vi. the 3'-end of 1.9 kb rna maps at 0.95 m.u. and is co-terminal with the ... | 1981 | 6174946 |
| further characterization of the discontinuities in cauliflower mosaic virus dna. | 1981 | 9222326 | |
| nucleotide sequence of naturally occurring deletion mutants of cauliflower mosaic virus. | the dnas of two isolates of strain cm4-184 of cauliflower mosaic virus, reported by other investigators to have deletions in different locations of the region ii-region iii portion of the genome, were found to give identical fragments when cleaved with bglii, bamhi, and hindiii restriction endonucleases. when these isolates of cm4-184 were cloned into the single-stranded vector m13mp7 followed by nucleotide sequence analyses, they had identical sequences. comparison of the sequences of such cm4- ... | 1981 | 18635076 |
| ultraviolet mapping of rna transcripts encoded by the cauliflower mosaic virus genome. | the transcription of the cauliflower mosaic virus (camv) genome was analyzed by ultraviolet (uv) mapping procedures to determine promoter sites and polarity of synthesis of "late" viral rna transcripts. the late transcripts include two major, discrete polyadenylated rnas, a large full-genome-length transcript and a 19 s rna, and nonpolyadenylated rna fragments smaller than the full-genome-length transcript. all late transcripts are derived from one strand of the camv genome, the alpha strand. wh ... | 1981 | 18635074 |
| structure of the 19 s rna transcript encoded by the cauliflower mosaic virus genome. | turnip leaves infected with cauliflower mosaic virus accumulate several virus-derived rna species. these include a large transcript (>25 s) and a 19 s transcript. r-looping and diazo-cellulose filter hybridization techniques show that the 19 s rna is derived from a contiguous segment of the camv genome which includes ecor1-b and -d dna fragments. this region encodes the synthesis of a nonvirion protein called p66. the 3' end of the 19 s transcript is polyadenylated and terminates near the single ... | 1981 | 18635053 |
| clones of cauliflower mosaic virus identified by molecular hybridization in turnip leaves. | mechanical inoculation of turnip leaves with cauliflower mosaic virus (camv) results after one to two weeks in the appearance on these leaves of local lesions. local lesions were detected by hybridization of radioactive camv dna with nucleic acid immobilized in leaf skeletons by solvent extraction, proteinase digestion, and alkali treatment. the pattern of lesions detected as dark circles on autoradiographs of the washed leaf skeletons was the same as that detected by staining of solvent-extract ... | 1981 | 24317821 |
| glycosylation of the cauliflower mosaic virus capsid polypeptide. | evidence is presented for the glycosylation of the cauliflower mosaic virus (camv) capsid polypeptide. fluorescent derivatives of concanavalin a (con a) were shown to bind specifically to the dissociated camv capsid polypeptides in polyacrylamide gels. con a also bound specifically to intact camv, so inhibiting the attachment of virus-specific antibodies. the role of carbohydrate residues in camv antigenicity is evaluated. | 1981 | 18635040 |
| characterisation of heparin-resistant complex formation and rna synthesis by wheat germ rna polymerases i, ii and iii, in vitro on cauliflower mosaic virus dna. | 1981 | 6163430 | |
| mapping of the coat protein gene of cauliflower mosaic virus by its expression in a prokaryotic system. | polypeptides recognized by antisera to cauliflower mosaic virus (camv) coat protein were found in lysates of escherichia coli cells that carry the complete camv genome on plasmid vectors. synthesis of this cross-reacting material in e. coli was detected when the complete camv dna sequence was present at either of two sites and in either orientation at one site of the cloning vector, suggesting prokaryotic promoter activity for some sequence within the viral dna. using an immunosorbent assay to s ... | 1982 | 18638811 |
| infection of turnip protoplasts with cauliflower mosaic virus dna. | 1982 | 18638813 | |
| nucleotide sequence of dna from an altered-virulence isolate d/h of the cauliflower mosaic virus. | the double-stranded dna from the isolate d/h with an altered virulence of the cauliflower mosaic virus (camv) contains 8016 bp. the dna is circular and possesses, like the dna of most camv strains, three sequence interruptions. the comparison of its sequence with the previously published sequences of two other camv strains (cabb-s and cm 1841) leads to the following conclusions: (1) the genetic organization of all three camv strains is identical with six potential genes (open reading frames) and ... | 1982 | 7152260 |
| transcription of cauliflower mosaic virus dna: detection of promoter sequences, and characterization of transcripts. | four rna transcripts encoded by cauliflower mosaic virus dna have been detected in the polyadenylated rna from virus-infected turnip leaves. two of these transcripts, the major 35s and the 8s species, have the same 5' termini, at nucleotide 7435. a viral dna fragment encompassing this region directs transcription initiation at this point in vitro. the 5' terminus of the 19s transcript is at nucleotide 5764, and a corresponding viral dna fragment also directs transcription initiation in vitro. th ... | 1982 | 7139714 |
| evidence for replication of cauliflower mosaic virus dna in plant nuclei. | cellular organelles of cauliflower mosaic virus-infected turnip plants were isolated, incubated with 32p-labeled deoxyribonucleoside triphosphates and assayed for their ability to synthesize virus-specific dna. only nuclei were found to synthesize a labeled dna product. this dna migrated during agarose gel electrophoresis at the same mobility as viral open circular dna. when purified by excision and phenol extraction from agarose gels followed by digestion with ecori restriction endonuclease, th ... | 1982 | 18638750 |
| a transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus dna isolated from infected turnip leaves. | purified nuclei from turnip leaves infected by cauliflower mosaic virus (camv) have been shown to contain a fraction of camv dna that consists of covalently closed circular molecules; possesses a nucleosome structure, based on sensitivity to micrococcal nuclease; and contains nuclear rna polymerase ii that selectively transcribes the coding strand of camv dna in vitro. our results suggest that the transcriptionally active camv dna is in the form of a minichromosome and that this dna does not con ... | 1982 | 7116445 |
| in vivo recombination of cauliflower mosaic virus dna. | ligation and recombination of the dna of cauliflower mosaic virus (camv) is demonstrated by the following experiments: (i) ligation: different noninfectious fragments of the camv genome (obtained after insertion into plasmid pbr322 followed by enzymatic excision) regained infectivity when mixtures of them were used to inoculate their host. the symptom appearance was delayed by comparison with a typical camv infection, and only the newly formed leaves were affected. (ii) recombination: pairs of n ... | 1982 | 16593187 |
| free cauliflower mosaic virus supercoiled dna in infected plants. | evidence is presented indicating that free viral dna exists in cauliflower mosaic virus-infected leaves. among the different viral dna forms detected in total dna extracts is a supercoiled form. we discuss its possible role in the replication process. | 1982 | 18635121 |
| structure and 5'-termini of the large and 19 s rna transcripts encoded by the cauliflower mosaic virus genome. | cauliflower mosaic virus-infected leaves accumulate two major rna species late in infection-a 19 s rna and a large rna transcript. we have found that the large transcript is approximately full genome length. the 3'-end of the large transcript is located at or near a site corresponding to the 3'-end of the 19 s rna. the 5'-end of the large transcript maps near its own 3'-end and only about 100 bp downstream from the terminator codon for coding region vi. the 5'-end of the large transcript is situ ... | 1982 | 18635118 |
| evidence for a 58-kilodalton polypeptide as precursor of the coat protein of cauliflower mosaic virus. | purified cauliflower mosaic virus subjected to agarose gel electrophoresis separated into two zones, slow and fast, with the latter consisting of less than 5-10% of the total. when these two types of virions were excised from gels and subjected to analysis in dodecyl sulfate impregnated gels, the fast component consisted mainly of the 58- and 44-kilodalton (kd) phosphorylated forms of the coat protein whereas the slow component contained only lower molecular weight forms of the same protein. pul ... | 1982 | 18635117 |
| identification by immunoprecipitation of cauliflower mosaic virus in vitro major translation product with a specific serum against viroplasm protein. | a highly specific antiserum was prepared against purified cauliflower mosaic virus viroplasm-protein (vmp). a virus specific in vitro major translation product (tpmaj), encoded by the 19s poly(a) rna fraction from cauliflower mosaic virus infected turnip leaves, was recognized by this antiserum. the n-terminal sequence of tpmaj corresponds to the sequence following the first in-phase initiation codon in gene vi of the cauliflower mosaic virus genome. both vmp and tpmaj have blocked termini and p ... | 1982 | 16453427 |
| intergenomic recombination events among pairs of defective cauliflower mosaic virus genomes in plants. | pairs of defective cauliflower mosaic virus genomes coinoculated in plants are rescued at high frequency by homologous, intergenomic recombination events. defective genomes may be rescued by one of at least two general types of recombination mechanisms. one mechanism appears to operate on linear dna molecules with complementary sticky ends. recombination of this type can be explained by the formation of mixed dimers (or concatamers) which can be resolved into normal genomes by a single crossover ... | 1982 | 7153685 |
| cauliflower mosaic virus on its way to becoming a useful plant vector. | 1982 | 6276092 | |
| mutagenesis of cauliflower mosaic virus. | a series of insertion mutants of cauliflower mosaic virus (camv) dna has been constructed in vitro. these insertions consist of a short dna sequence (10 or 22 bp) containing a restriction endonuclease site (smai) not represented on the viral dna. viral infectivity was analyzed by inoculating plants with the mutated cloned viral dna and observing symptoms. insertions within orfvii, and in one site within the large intergenic region, did not interfere with viral infectivity, whilst insertions with ... | 1983 | 6319236 |
| insertional mutagenesis of the cauliflower mosaic virus genome. | a series of small insertions has been introduced into the various translational reading frames of the dna of a "severe" strain of cauliflower mosaic virus (camv). a selectable gene (the kanamycin phosphotransferase gene of tn903), flanked by a series of symmetrically arranged cloning sites taken from m13mp7, was used to prepare the site-specific mutants. in-phase insertions of 12 or 30 bp, which introduced unique sali sites into reading regions i, iii, iv, v and into the amino-proximal portion o ... | 1983 | 6363213 |
| sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis b virus and cauliflower mosaic virus. | in infected cells, the rna genomes of rna tumour viruses are copied into dna by a virus-encoded reverse transcriptase enzyme. this transfer of information from rna into dna was thought to be a unique feature of rna tumour viruses, but recent results suggest it may be a more general strategy. hepatitis b virus (hbv) has a double-stranded dna genome, and it has recently been shown that the minus dna strand of the hbv genome is copied from a plus-strand rna template, leading to the suggestion that ... | 1983 | 6195530 |
| the application of spot hybridization to the detection of dna and rna viruses in plant tissues. | a solid-phase nucleic acid hybridization technique for the detection of dna and rna viruses in plant tissues is described. the method involves spotting crude samples onto nitrocellulose and using 12p-labelled dna hybridization probes. the limit of sensitivity is 5-20 pg virus/spot or approximately 5 micrograms/g leaf tissue. the method is quantitative for dna viruses in crude homogenates, but not for rna viruses. the amount of cauliflower mosaic virus in infected leaves and protoplasts was estim ... | 1983 | 6863467 |
| physical maps of the genomes of dahlia mosaic virus and mirabilis mosaic virus-two members of the caulimovirus group. | the nucleic acids of dahlia mosaic virus (damv) and mirabilis mosaic virus (mmv) have been isolated and compared with the native dna of cauliflower mosaic virus (camv). the native dnas of these viruses separated into circular and linear molecules during gel electrophoresis to produce patterns nearly identical to those of camv. the dnas of damv and mmv were cloned in bacteria and used for mapping the cleavage sites for 14 different restriction endonucleases. these sites were confirmed with native ... | 1983 | 18644277 |
| nuclei purified from cauliflower mosaic virus-infected turnip leaves contain subgenomic, covalently closed circular cauliflower mosaic virus dnas. | nuclei isolated from cauliflower mosaic virus (camv) infected turnip leaves contain subgenomic camv dna species in addition to the genome length camv dna. these subgenomic camv dna species are present as covalently closed circles (form i), relaxed circles (form ii) and linear (form iii) molecules. the subgenomic form i dna species range in size from about 10% of genome length to nearly genome length. these subgenomic dna species appear in tissue infected with cloned camv dna, indicating that the ... | 1983 | 6672775 |
| selective transcription of a cloned cauliflower mosaic virus dna fragment in vitro by soybean rna polymerase ii in the presence of dinucleotide primers. | transcription of a cloned cauliflower mosaic virus (camv) dna fragment (plasmid pca 8) was studied at a low enzyme: dna ratio. preincubation with purine nucleoside triphosphates leads to essentially random transcription, while in the presence of a dinucleoside monophosphate and a purine nucleoside triphosphate in the preincubation medium certain combinations prime preferential transcription of the eucaryotic moiety of the chimeric plasmid. characterisation of transcription primed by the most eff ... | 1983 | 6686138 |
| characterization and preliminary mapping of cauliflower mosaic virus transcripts. | using northern blot analysis we have studied the transcription of the cm4-184 ga. strain of cauliflower mosaic virus (camv) dna. this analysis reveals that this camv strain, like the cabb-bs and the cabb b-ji strains, produces both a genomic length transcript and a 1900-nucleotide (nt) transcript during infection. in addition, we detect an 1800-nt pa+ transcript mapping primarily to the ecori-c region of the virus, and three apparent minor viral-specific pa + rnas of 4900, 4500, and 4300 nt. we ... | 1983 | 6662362 |
| helper component for aphid transmission encoded by region ii of cauliflower mosaic virus dna. | a deletion mutant of cauliflower mosaic virus (camv) isolate ny8153 deficient in aphid transmissibility was constructed by bal-31 exonuclease treatment of xhoi linearized pcms31 (a plasmid containing the entire camv genome cloned in the sali site of pbr322), followed by ligation. the resulting mutant, psa103, lacked about 100 by from the putative protein-coding region ii of camv dna. in turnips there was no difference in the number and appearance of starch lesions or hybridization lesions, or in ... | 1983 | 18639087 |
| detection of partially proteolysed cauliflower mosaic virus coat protein in infected leaf tissue by western blotting. | cauliflower mosaic virus (camv) capsid polypeptides were detected by immunoelectroblotting ('western blotting') 10-16 days after infection of chinese cabbage leaves. the predominant polypeptides detected had molecular weights of 42,000 and 37,000 suggesting that in vivo proteolysis of the 55,000-58,000 molecular weight coat protein had taken place. the use of laboratory-made nitrocellulose membranes for western blotting is reported. phosphate-sds buffer was more suitable than tris-glycine buffer ... | 1983 | 6352722 |
| aphid transmission and a polypeptide are specified by a defined region of the cauliflower mosaic virus genome. | infection of young turnip leaves with an aphid-transmissible isolate, cabb b-ji, of cauliflower mosaic virus (camv) causes synthesis of an mr 18 000 polypeptide (p18) which co-purifies with virus inclusion bodies. this polypeptide is not detectable in leaves infected with either of two aphid non-transmissible isolates. campbell and cm4-184. construction in vitro, of hybrid genomes between cabb b-ji and campbell isolates demonstrates that aphid transmissibility and presence of p18 is dependent on ... | 1983 | 6311674 |
| involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. | a model is presented according to which cauliflower mosaic virus (camv) dna is replicated via an rna intermediate. the model explains the typical s1 nuclease-sensitive sites in mature camv dna, the occurrence of the large, terminally redundant transcript, the local separation of camv transcription and camv assembly, the abundance of knotted camv dna forms, and the high recombination frequency. a site of perfect homology to plant trna was detected. extracts from a mixture of nuclei and inclusion ... | 1983 | 6191868 |
| characterisation of cauliflower mosaic virus dna forms isolated from infected turnip leaves. | several different forms of cauliflower mosaic virus (camv) dna were detected in nucleic acid preparations from camv-infected turnip leaves. as well as supercoiled and open-circular molecules, various linear dna structures were identified. the relative amounts of these dna forms varied in plants infected with different camv isolates. restriction enzyme mapping and one- and two-dimensional gel electrophoresis revealed the presence of linear molecules apparently formed by breaks in the second stran ... | 1983 | 6300783 |
| a small dna molecule containing covalently-linked ribonucleotides originates from the large intergenic region of the cauliflower mosaic virus genome. | we have detected a small dna molecule (sa-dna), 725 nucleotides long, in cauliflower mosaic virus (camv)-infected, but not non-infected, turnip leaves. alkali and rnase a treatments shortened sa-dna by 100 nucleotides and we conclude that it contains covalently-linked ribonucleotides. this dna co-purified with cellular polyadenylated rna. it is complementary to the beta-strand of camv dna and of opposite polarity to rnas transcribed from the alpha-strand. hybridisation studies suggest that sa-dn ... | 1983 | 6298717 |
| uncut recombinant plasmids bearing nested cauliflower mosaic virus genomes infect plants by intragenomic recombination. | experiments are described which show that recombinant plasmids bearing cauliflower mosaic virus genomes flanked by homologous viral dna arms ('nested' viral genomes) can infect plants without prior excision of the camv genome. infectious viral genomes are excised from such recombinant plasmids within the plant by a process of intramolecular recombination. the frequency of infection, and presumably recombination increase with the length of the homologous dna flanking the camv genome. this mechani ... | 1983 | 24318101 |
| observations concerning the discontinuous dnas of cauliflower mosaic virus. | the double-stranded circular dna encapsidated within cauliflower mosaic virus (camv) particles contains three single-stranded discontinuities, two in one strand and one in the other, so that, upon denaturation, three linear single-stranded dnas are produced. here we show that a fourth much smaller single-stranded dna, termed alpha1, is also present in denatured camv dna preparations. the 5' extremity of alpha1 is identical to that of the alpha-strand, the strand of dna possessing only one interr ... | 1983 | 11894938 |
| electron microscopic studies of the different topological forms of the cauliflower mosaic virus dna: knotted encapsidated dna and nuclear minichromosome. | cauliflower mosaic virus (camv) dna exists under different topological forms in infected plants. first, the population of encapsidated camv dna molecules appears heterogeneous when analysed by gel electrophoresis. the electron microscopic study reported here reveals that camv virion dna contains simple and multiple topological knots. second, a supercoiled dna form never found in virions exists as a chromatin-like nucleoprotein complex with nucleosome subunits in the nuclei of infected leaves. th ... | 1983 | 6628362 |
| multiple, discrete 35s transcripts of cauliflower mosaic virus. | we have devised a modification of the berk and sharp procedure that allows us to detect the presence of a diverse population of 35s full-length, genomic viral transcripts of cauliflower mosaic virus (camv). using this procedure we have been able to identify and characterize four such 35s transcripts. the first 35s rna that we have mapped, 35s-1, is a slightly longer than full-length transcript, beginning approximately at nucleotide 7500 and ending at nucleotide 7650 and corresponds to a previous ... | 1983 | 6319527 |
| isolation and characterization of faithful and altered clones of the genomes of cauliflower mosaic virus isolates cabb b-ji, cm4-184, and bari i. | full-length genomes of cauliflower mosaic virus (camv) isolates cabb b-ji, cm4-184, and bari i have been cloned in the salgi site of plasmid pat 153. the cloned dnas were characterized by restriction mapping and infectivity assays. all the sites present in the virion dnas were found in the cloned dnas. comparison of restriction maps with those of dna from two other isolates which have been recently completely sequenced revealed a close relationship among the different isolates. some of the clone ... | 1983 | 6300943 |
| primary structural comparison of rna-dependent polymerases from plant, animal and bacterial viruses. | possible alignments for portions of the genomic codons in eight different plant and animal viruses are presented: tobacco mosaic, brome mosaic, alfalfa mosaic, sindbis, foot-and-mouth disease, polio, encephalomyocarditis, and cowpea mosaic viruses. since in one of the viruses (polio) the aligned sequence has been identified as an rna-dependent polymerase, this would imply the identification of the polymerases in the other viruses. a conserved fourteen-residue segment consisting of an asp-asp seq ... | 1984 | 6207485 |
| expression of a putative plant viral gene in escherichia coli. | a recombinant plasmid, pcb300, was constructed which carries a cauliflower mosaic virus (camv) dna insert corresponding to nucleotides 1825-2280, including the coding sequence (1830-2219) of open reading frame iii (orf iii). this camv dna insert was fused with the amino-terminal portion of the beta-galactosidase gene. transcription of the hybrid gene is controlled by the lac promoter, which is repressed in escherichia coli strain jm103 and can be induced by isopropylthio-beta-d-galactoside (iptg ... | 1984 | 6098536 |
| a cauliflower mosaic virus promoter directs expression of kanamycin resistance in morphogenic transformed plant cells. | the promoter region of the camv inclusion body protein gene was modified for use in chimeric gene fusions. the modified promoter was used to construct a selectable marker for plant transformation based on the tn 5 kanamycin resistance gene. this chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of agrobacterium tumefaciens. both types of transformation produced kanamycin-resistant cell lines. the resistant cell lines derived from the deoncogeniz ... | 1984 | 6099400 |
| a dna polymerase activity is associated with cauliflower mosaic virus. | a dna polymerase activity is found within the cauliflower mosaic virus (camv) particle. analysis of the reaction product reveals that the linear form of the virion dna is preferentially labelled. the molecular weight of the dna polymerase as determined on an "activity gel" is 76 kda. | 1984 | 6514573 |
| rna-dependent dna polymerase activity in cauliflower mosaic virus-infected plant leaves. | cauliflower mosaic virus (camv) is a plant dna with an 8-kb circular double-stranded genome. camv-specific dna and rna molecules present in infected brassica cells share some structural features with dnas and rnas of retroviruses and hepatitis b virus. this led to the hypothesis that camv replication occurs via reverse transcription of an rna intermediate. here we report the first characterization of a new dna polymerase activity, specific to camv-infected tissues. a subcellular fraction of infe ... | 1984 | 16453500 |
| direct gene transfer to plants. | evidence for direct, gene-mediated stable genetic transformation of plant cells of nicotiana tabacum is presented. a selectable hybrid gene comprising the protein coding region of the tn5 aminoglycoside phosphotransferase type ii gene under control of cauliflower mosaic virus gene vi expression signals was introduced into plant protoplasts as part of an escherichia coli plasmid. the gene was stably integrated into plant genomic dna and constitutively expressed in selected, drug resistant, protop ... | 1984 | 16453573 |
| initiation of translation of the cauliflower mosaic virus genome from a polycistronic mrna: evidence from deletion mutagenesis. | mutants with deletions in the open reading frame (orf) vii to i region of the cauliflower mosaic virus genome were constructed in vitro and tested for infectivity by inoculation of plants. sequences within orf vii and the intergenomic region between orfs vii and i are not required for viral infectivity. nevertheless, the location of translation initiation and termination codons within this region governs the infectivity of the virus. firstly, translation beginning at the initiation codon of orf ... | 1984 | 16453575 |
| use of the immuno-gold technique for in situ localization of cauliflower mosaic virus (camv) particles and the major protein of the inclusion bodies. | we show that the immuno-gold technique adapted to electron microscopy is a sensitive method for in situ localization of viral proteins in plant cells. using antisera raised against cauliflower mosaic virus and the viroplasm major protein (vmp) we obtained a selective labelling of the viral particles and of the viroplasmic matrix in infected cells. | 1984 | 6397480 |
| detection in vivo of a new gene product (gene iii) of cauliflower mosaic virus. | cauliflower mosaic virus dna contains six major open reading frames (orfs). as only the mrna corresponding to the transcription of gene vi and its translation product have been isolated, the identification in infected plants of products corresponding to the five other putative genes remains to be established. the present paper reports the detection of an orf iii product by means of antibodies raised against an nh(2)-terminal synthetic peptide of 19 amino acids corresponding to a sequence predict ... | 1984 | 16593524 |
| study on the genome of cauliflower mosaic virus (xinjiang isolate). restriction mapping of virion and cloned viral dna. | the genomic dna of camv (xinjiang isolate) was mapped on the virion dna with a number of restriction endonucleases by double digestions and partial digestions of one-end 32p-labelled fragments. it contained the unique sites of bgli, sali and xhoi. bamhi and hhai each cut it at two sites, ecori at five sites (three of which were located), hpaii at seven sites, bglii at eight sites, and haeiii at ten sites. the sites of all the enzymes used above, as well as hindiii which cut at ten sites were als ... | 1984 | 6098967 |