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ab-qtl analysis in winter wheat: ii. genetic analysis of seedling and field resistance against leaf rust in a wheat advanced backcross population.the present study aimed to localize exotic quantitative trait locus (qtl) alleles for the improvement of leaf rust (p. triticina) resistance in an advanced backcross (ab) population, b22, which is derived from a cross between the winter wheat cultivar batis (triticum aestivum) and the synthetic wheat accession syn022l. the latter was developed from hybridization of t. turgidum ssp. dicoccoides and t. tauschii. altogether, 250 bc2f3 lines of b22 were assessed for seedling resistance against the l ...018338154
green manure and long-term fertilization effects on soil zinc and cadmium availability and uptake by wheat (triticum aestivum l.) at different growth stages.zinc (zn) deficiency in human populations depending on cereals as a main source of zn is a global malnutrition problem. in this field study, we investigated the potential of green manure application to increase soil zn availability and wheat grain zn concentrations (biofortification) on a luvisol with different long-term fertilizer management. we also studied cadmium (cd), as wheat is a major contributor of this undesired non-essential element to human diets. clover (trifolium alexandrinum l.), ...028525939
transcriptome of wheat inflorescence development from spikelet initiation to floral patterning.early reproductive development in cereals is crucial for final grain number per spike, and hence the yield potential of the crop. to date, however, no systematic analyses of gene expression profiles during this important process have been conducted for common wheat (triticum aestivum). here, we studied the transcriptome profiles at four stages of early wheat reproductive development, from spikelet initiation to floral organ differentiation. k-means clustering and stage-specific transcript identi ...028515146
deacclimation may be crucial for winter survival of cereals under warming climate.climate warming can change the winter weather patterns. warmer temperatures during winter result in a lower risk of extreme freezing events. on the other hand the predicted warm gaps during winter will decrease their freezing tolerance. both contradict effects will affect winter survival but their resultant effect is unclear. in this paper, we demonstrate that climate warming may result in a decrease in winter survival of plants. a field study of winterhardiness of common wheat and triticale was ...028167038
silicon availability modifies nutrient use efficiency and content, c:n:p stoichiometry, and productivity of winter wheat (triticum aestivum l.).silicon (si) is known as beneficial element for graminaceous plants. the importance of si for plant functioning of cereals was recently emphasized. however, about the effect of si availability on biomass production, grain yield, nutrient status and nutrient use efficiency for wheat (triticum aestivum l.), as one of the most important crop plants worldwide, less is known so far. consequently, we assessed the effect of a broad range of supply levels of amorphous sio2 on wheat plant performance. ou ...028094308
salicylic acid mediated growth, physiological and proteomic responses in two wheat varieties under drought stress.salicylic acid (sa) induced drought tolerance can be a key trait for increasing and stabilizing wheat production. these sa induced traits were studied in two triticum aestivum l. varieties; drought tolerant, kundan and drought sensitive, lok1 under two different water deficit regimes: and rehydration at vegetative and flowering stages. sa alleviated the negative effects of water stress on photosynthesis more in kundan. sa induced defense responses against drought by increasing antioxidative enzy ...028511789
pouteria torta epicarp as a useful source of α-amylase inhibitor in the control of type 2 diabetes.type 2 diabetes plays a major role in public health, affecting about 400 million adults. one of the used strategies to control type 2 diabetes is the inhibition of α-amylase activity to reduce post-prandial blood glucose levels. therefore, in past decades, the search of new α-amylase inhibitors has led to the evaluation of natural products as a source of these compounds. pouteria torta (sapotaceae) is widespread in brazil and bears edible fruits. epicarp and pulp crude extracts of fresh fruits w ...028288931
amino acid profiles and digestible indispensable amino acid scores of proteins from the prioritized key foods in bangladesh.concentrations of standard amino acids were determined in the composite samples (representing 30 agro-ecological zones of bangladesh) of six prioritized key dietary protein sources: oryza sativa (rice), triticum aestivum (wheat flour), lens culinaris (lentils), pangusius pangusius (pangas), labeo rohita (rohu) and oreochromis mossambicus (tilapia). digestible indispensable amino acid scores (diaas) was calculated using published data on amino acids' digestibility to evaluate the protein quality ...027451158
phytotoxicity of three plant-based biodiesels, unmodified castor oil, and diesel fuel to alfalfa (medicago sativa l.), lettuce (lactuca sativa l.), radish (raphanus sativus), and wheatgrass (triticum aestivum).the wide use of plant-based oils and their derivatives, in particular biodiesel, have increased extensively over the past decade to help alleviate demand for petroleum products and improve the greenhouse gas emissions profile of the transportation sector. biodiesel is regarded as a clean burning alternative fuel produced from livestock feeds and various vegetable oils. although in theory these animal and/or plant derived fuels should have less environmental impact in soil based on their simplifi ...026283287
heavy metal and metalloid concentrations in components of 25 wheat (triticum aestivum) varieties in the vicinity of lead smelters in henan province, china.soil contamination and human impacts have been reported in the vicinity of lead (pb) smelters in henan, china. however, no information is available on crop uptake of soil contaminants near these smelters. grains, glume, rachis, and stem/leaf samples of 25 wheat (triticum aestivum) varieties were collected from a small, smelter-impacted agricultural area of beishe village, henan province, and were analyzed for arsenic (as), cadmium (cd), copper (cu), pb, and zinc (zn) concentrations. the study ai ...026661959
characteristics of cadmium uptake and membrane transport in roots of intact wheat (triticum aestivum l.) seedlings.wheat is one of several cereals that is capable of accumulating higher amounts of cd in plant tissues. it is important to understand the cd(2+) transport processes in roots that result in excess cd accumulation. traditional destructive technologies have limited capabilities in analyzing root samples due to methodological limitations, and sometimes may result in false conclusions. the mechanisms of cd(2+) uptake into the roots of wheat seedlings (triticum aestivum l.) were investigated by assessi ...028012673
molecular diversity of α-gliadin expressed genes in genetically contrasted spelt (triticum aestivum ssp. spelta) accessions and comparison with bread wheat (t. aestivum ssp. aestivum) and related diploid triticum and aegilops species.the gluten proteins of cereals such as bread wheat (triticum aestivum ssp. aestivum) and spelt (t. aestivum ssp. spelta) are responsible for celiac disease (cd). the α-gliadins constitute the most immunogenic class of gluten proteins as they include four main t-cell stimulatory epitopes that affect cd patients. spelt has been less studied than bread wheat and could constitute a source of valuable diversity. the objective of this work was to study the genetic diversity of spelt α-gliadin transcri ...027942245
optimization of the malting process for nutritional improvement of finger millet and amaranth flours in the infant weaning food industry.malting is a beneficial approach to improve the nutritional value of cereals used in infant preparations. malted finger millet and amaranth might be considered as potentially appropriate gluten-free alternatives for common wheat-based weaning products, especially in case of those suffering from celiac disease. in this study, the effects of germination temperature and duration on the main nutrients of malted finger millet and amaranth, are evaluated and optimized. grains were germinated for 24, 3 ...027905218
mycotoxin and fungicide residues in wheat grains from fungicide-treated plants measured by a validated lc-ms method.wheat (triticum aestivum) is an annual crop, cultivated in the winter and spring and susceptible to several pathogens, especially fungi, which are managed with fungicides. it is also one of the most consumed cereals, and can be contaminated by mycotoxins and fungicides. the objective of this study was to validate an analytical method by lc-ms for simultaneous determination of mycotoxins and fungicide residues in wheat grains susceptible to fusarium head blight treated with fungicides, and to eva ...027855933
ancient wheat and health: a legend or the reality? a review on kamut khorasan wheat.after wwii, the industrialized agriculture selected modern varieties of triticum turgidum spp. durum and spp. aestivum (durum wheat and common wheat) based on higher yields and technological characteristics. nowadays, the use of whole ancient grains and pseudo cereals is considered nutritionally important. how ancient grains have positive effects is not entirely known, the fragmentation of the scientific knowledge being also related to the fact that ancient grains are not a homogeneous category. ...027790934
the defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias.bread wheat (triticum aestivum l.) is an allopolyploid species containing three ancestral genomes. therefore, three homoeologous copies exist for the majority of genes in the wheat genome. whether different homoeologs are differentially expressed (homoeolog expression bias) in response to biotic and abiotic stresses is poorly understood. in this study, we applied a rna-seq approach to analyse homoeolog-specific global gene expression patterns in wheat during infection by the fungal pathogen fusa ...027735125
mobility and plant availability of risk elements in soil after long-term application of farmyard manure.crop rotation long-term field experiments were established in 1955 and 1956 at three locations in the czech republic (čáslav, ivanovice, and lukavec) differing in their climatic and soil physicochemical properties. the effect of long-term application of farmyard manure and farmyard manure + npk treatments on plant-available, easily mobilizable, potentially mobilizable, and pseudo-total contents of arsenic (as), cadmium (cd), copper (cu), lead (pb), and zinc (zn) contents in soils (in 2013) as we ...027614644
comparison of volatiles profile and contents of trichothecenes group b, ergosterol, and atp of bread wheat, durum wheat, and triticale grain naturally contaminated by mycobiota.in natural conditions cereals can be infested by pathogenic fungi. these can reduce the grain yield and quality by contamination with mycotoxins which are harmful for plants, animals, and humans. to date, performed studies of the compounds profile have allowed for the distinction of individual species of fungi. the aim of this study was to determine the profile of volatile compounds and trichothecenes of group b, ergosterol, adenosine triphosphate content carried out on a representative sample o ...027597856
protective effects of id331 triticum monococcum gliadin on in vitro models of the intestinal epithelium.a growing interest in developing new strategies for preventing coeliac disease has motivated efforts to identify cereals with null or reduced toxicity. in the current study, we investigate the biological effects of id331 triticum monococcum gliadin-derived peptides in human caco-2 intestinal epithelial cells. triticum aestivum gliadin derived peptides were employed as a positive control. the effects on epithelial permeability, zonulin release, viability, and cytoskeleton reorganization were inve ...027374565
allelic variation in developmental genes and effects on winter wheat heading date in the u.s. great plains.heading date in wheat (triticum aestivum l.) and other small grain cereals is affected by the vernalization and photoperiod pathways. the reduced-height loci also have an effect on growth and development. heading date, which occurs just prior to anthesis, was evaluated in a population of 299 hard winter wheat entries representative of the u.s. great plains region, grown in nine environments during 2011-2012 and 2012-2013. the germplasm was evaluated for candidate genes at vernalization (vrn-a1, ...027058239
antioxidant responses of wheat plants under stress.currently, food security depends on the increased production of cereals such as wheat (triticum aestivum l.), which is an important source of calories and protein for humans. however, cells of the crop have suffered from the accumulation of reactive oxygen species (ros), which can cause severe oxidative damage to the plants, due to environmental stresses. ros are toxic molecules found in various subcellular compartments. the equilibrium between the production and detoxification of ros is sustain ...027007891
mitogen-activated protein kinase kinase 3 regulates seed dormancy in barley.seed dormancy has fundamental importance in plant survival and crop production; however, the mechanisms regulating dormancy remain unclear [1-3]. seed dormancy levels generally decrease during domestication to ensure that crops successfully germinate in the field. however, reduction of seed dormancy can cause devastating losses in cereals like wheat (triticum aestivum l.) and barley (hordeum vulgare l.) due to pre-harvest sprouting, the germination of mature seed (grain) on the mother plant when ...026948880
leaf length tracker: a novel approach to analyse leaf elongation close to the thermal limit of growth in the field.leaf growth in monocot crops such as wheat and barley largely follows the daily temperature course, particularly under cold but humid springtime field conditions. knowledge of the temperature response of leaf extension, particularly variations close to the thermal limit of growth, helps define physiological growth constraints and breeding-related genotypic differences among cultivars. here, we present a novel method, called 'leaf length tracker' (llt), suitable for measuring leaf elongation rate ...026818912
genome-wide linkage mapping of qtl for adult-plant resistance to stripe rust in a chinese wheat population linmai 2 × zhong 892.stripe rust is one of the most devastating diseases of wheat (triticum aestivum) worldwide. adult-plant resistance (apr) is an efficient approach to provide long-term protection of wheat from the disease. the chinese winter wheat cultivar zhong 892 has a moderate level of apr to stripe rust in the field. to determine the inheritance of the apr resistance in this cultivar, 273 f6 recombinant inbred lines (rils) were developed from a cross between linmai 2 and zhong 892. the rils were evaluated fo ...026714310
the linear plastid chromosomes of maize: terminal sequences, structures, and implications for dna replication.the structure of a chromosomal dna molecule may influence the way in which it is replicated and inherited. for decades plastid dna (ptdna) was believed to be circular, with breakage invoked to explain linear forms found upon extraction from the cell. recent evidence indicates that ptdna in vivo consists of linear molecules with discrete termini, although these ends were not characterized. we report the sequences of two terminal regions, end1 and end2, for maize (zea mays l.) ptdna. we describe s ...026650613
uncoupling of sodium and chloride to assist breeding for salinity tolerance in crops.the separation of toxic effects of sodium (na(+)) and chloride (cl(-)) by the current methods of mixed salts and subsequent determination of their relevance to breeding has been problematic. we report a novel method (na(+) humate) to study the ionic effects of na(+) toxicity without interference from cl(-), and ionic and osmotic effects when combined with salinity (nacl). three cereal species (hordeum vulgare, triticum aestivum and triticum turgidum ssp. durum with and without the na(+) exclusio ...026607560
genome-wide linkage mapping of flour color-related traits and polyphenol oxidase activity in common wheat.fifty-six qtl for flour color-related traits and polyphenol oxidase activity were identified using a genome-wide linkage mapping of data from a ril population derived from a gaocheng 8901/zhoumai 16 cross.026602234
"rolled-upness": phenotyping leaf rolling in cereals using computer vision and functional data analysis approaches.the flag leaf of a wheat (triticum aestivum l.) plant rolls up into a cylinder in response to drought conditions and then unrolls when leaf water relations improve. this is a desirable trait for extending leaf area duration and improving grain size particularly under drought. but how do we quantify this phenotype so that different varieties of wheat or different treatments can be compared objectively since this phenotype can easily be confounded with inter-genotypic differences in root-water upt ...026583042
diversifying crops for food and nutrition security - a case of teff.there are more than 50000 known edible plants in the world, yet two-thirds of global plant-derived food is provided by only three major cereals - maize (zea mays), wheat (triticum aestivum) and rice (oryza sativa). the dominance of this triad, now considered truly global food commodities, has led to a decline in the number of crop species contributing to global food supplies. our dependence on only a few crop species limits our capability to deal with challenges posed by the adverse effects of c ...026456883
metabolism of the fusarium mycotoxins t-2 toxin and ht-2 toxin in wheat.to investigate the metabolic fate of ht-2 toxin (ht2) and t-2 toxin (t2) in wheat (triticum aestivum l.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography-high resolution mass spectrometry was performed. in total, 11 ht2 and 12 t2 derived in planta biotransformation products were annotated putatively. in addition to previously reported mono- and diglucosylated forms of ht2, evidence for the formation of ht2-malonyl-glucoside and feruloyl-t2, as well a ...026278508
mass spectrometry-based analysis of whole-grain phytochemicals.whole grains are a rich source of several classes of phytochemicals, such as alkylresorcinols, benzoxazinoids, flavonoids, lignans, and phytosterols. a high intake of whole grains has been linked to a reduced risk of some major noncommunicable diseases, and it has been postulated that a complex mixture of phytochemicals works in synergy to generate beneficial health effects. mass spectrometry, especially when coupled with liquid chromatography, is a widely used method for the analysis of phytoch ...026167744
anthocyanins in purple and blue wheat grains and in resulting bread: quantity, composition, and thermal stability.the anthocyanin composition of blue (triticum aestivum l., cv. skorpion) and purple wheat (triticum aethiopicum jakubz cv. abyssinskaja arrasajta cv. abyssinskaja arrasajta), cultivated in the czech republic, and of the prepared whole blue and purple wheat bread was determined. in blue and purple wheat, 19 and 26 anthocyanins, respectively, were tentatively identified by liquid chromatography and mass spectrometry. the total content of anthocyanins determined in blue and purple wheat was 9.26 an ...026088282
is it necessary to split nitrogen fertilization for winter wheat? on-farm research on luvisols in south-west germany.mineral nitrogen (n) fertilization in cereals is commonly split into three or four applications. in order to simplify n fertilization, a single n application either broadcast or placed on the soil surface was compared to conventionally split fertilization for winter wheat (triticum aestivum l.). the 4-year experiment (2007-2010) was performed using a participatory approach on farmers' fields on deep loamy soils (luvisols) in south-west germany. grain yield and crude protein contents differed onl ...026063931
heat damage and in vitro starch digestibility of puffed wheat kernels.the effect of processing conditions on heat damage, starch digestibility, release of advanced glycation end products (ages) and antioxidant capacity of puffed cereals was studied. the determination of several markers arising from maillard reaction proved pyrraline (pyr) and hydroxymethylfurfural (hmf) as the most reliable indices of heat load applied during puffing. the considerable heat load was evidenced by the high levels of both pyr (57.6-153.4 mg kg(-1) dry matter) and hmf (13-51.2 mg kg(-1 ...026041194
folate distribution in barley (hordeum vulgare l.), common wheat (triticum aestivum l.) and durum wheat (triticum turgidum durum desf.) pearled fractions.wholegrain cereals are an important source of folates. in this study, total folate was analysed in pearled fractions of barley and wheat cultivars employing aoac official method 2004.05. in particular, the distribution of folate in the kernels was evaluated in three barley cultivars (two hulled types and a hulless one as well as two- and six-row types) and in a common and a durum wheat cultivar.026018777
transcriptional and metabolic alternations rebalance wheat grain storage protein accumulation under variable nitrogen and sulfur supply.wheat (triticum aestivum l.) grain storage proteins (gsps) are major determinants of flour end-use value. biological and molecular mechanisms underlying the developmental and nutritional determination of gsp accumulation in cereals are as yet poorly understood. here we timed the accumulation of gsps during wheat grain maturation relative to changes in metabolite and transcript pools in different conditions of nitrogen (n) and sulfur (s) availability. we found that the n/s supply ratio modulated ...025996785
post-head-emergence frost in wheat and barley: defining the problem, assessing the damage, and identifying resistance.radiant frost is a significant production constraint to wheat (triticum aestivum) and barley (hordeum vulgare), particularly in regions where spring-habit cereals are grown through winter, maturing in spring. however, damage to winter-habit cereals in reproductive stages is also reported. crops are particularly susceptible to frost once awns or spikes emerge from the protection of the flag leaf sheath. post-head-emergence frost (phef) is a problem distinct from other cold-mediated production con ...025873656
proteomic profiling of 16 cereal grains and the application of targeted proteomics to detect wheat contamination.global proteomic analysis utilizing sds-page, western blotting and lc-ms/ms of total protein and gluten-enriched extracts derived from 16 economically important cereals was undertaken, providing a foundation for the development of ms-based quantitative methodologies that would enable the detection of wheat contamination in foods. the number of proteins identified in each grain correlated with the number of entries in publicly available databases, highlighting the importance of continued advances ...025873154
extractability and chromatographic characterization of wheat (triticum aestivum l.) bran protein.about 70% of the protein for human consumption is derived from plants, with cereals as the most important source. wheat bran protein has a more balanced amino acid profile than that of flour. we here for the first time report the amino acid, size exclusion, and sds-page profiles of bran osborne protein fractions (opfs). moreover, we also investigated how opfs are affected when physical barriers which entrap proteins in bran tissues are removed. albumin/globulin is the most abundant opf. it is ri ...025854625
(1)h-nmr screening for the high-throughput determination of genotype and environmental effects on the content of asparagine in wheat grain.free asparagine in cereals is known to be the precursor of acrylamide, a neurotoxic and carcinogenic product formed during cooking processes. thus, the development of crops with lower asparagine is of considerable interest to growers and the food industry. in this study, we describe the development and application of a rapid (1)h-nmr-based analysis of cereal flour, that is, suitable for quantifying asparagine levels, and hence acrylamide-forming potential, across large numbers of samples. the sc ...025816894
genome-wide characterization of developmental stage- and tissue-specific transcription factors in wheat.wheat (triticum aestivum) is one of the most important cereal crops, providing food for humans and feed for other animals. however, its productivity is challenged by various biotic and abiotic stresses such as fungal diseases, insects, drought, salinity, and cold. transcription factors (tfs) regulate gene expression in different tissues and at various developmental stages in plants and animals, and they can be identified and classified into families according to their structural and specialized ...025766308
colonisation of winter wheat grain by fusarium spp. and mycotoxin content as dependent on a wheat variety, crop rotation, a crop management system and weather conditions.field experiments were conducted during three consecutive growing seasons (2007/08, 2008/09 and 2009/10) with four winter wheat (triticum aestivum l.) cultivars - 'bogatka', 'kris', 'satyna' and 'tonacja' - grown on fields with a three-field crop rotation (winter triticale, spring barley, winter wheat) and in a four-field crop rotation experiment (spring wheat, spring cereals, winter rapeseed, winter wheat). after the harvest, kernels were surface disinfected with 2% naocl and then analysed for ...025705931
does morphological and anatomical plasticity during the vegetative stage make wheat more tolerant of water deficit stress than rice?water scarcity and the increasing severity of water deficit stress are major challenges to sustaining irrigated rice (oryza sativa) production. despite the technologies developed to reduce the water requirement, rice growth is seriously constrained under water deficit stress compared with other dryland cereals such as wheat (triticum aestivum). we exposed rice cultivars with contrasting responses to water deficit stress and wheat cultivars well adapted to water-limited conditions to the same moi ...025614066
chromowiz: a web tool to query and visualize chromosome-anchored genes from cereal and model genomes.over the last years reference genome sequences of several economically and scientifically important cereals and model plants became available. despite the agricultural significance of these crops only a small number of tools exist that allow users to inspect and visualize the genomic position of genes of interest in an interactive manner.025491094
practical method for the confirmation of authentic flours of different types of cereals and pseudocereals.gas chromatography with mass spectrometry was used to perform a qualitative analysis of the liposoluble flour extract of different types of cereals (bread wheat and spelt) and pseudocereals (amaranth and buckwheat). in addition to major fatty acids, the liposoluble extract also contained minor fatty acids with more than 20 carbon atoms, higher hydrocarbons and phytosterols. tmsh (trimethylsulfonium hydroxide, 0.2 mol/l in methanol) was used as a trans-esterification reagent. in a trans-esterific ...025442559
triticeae resources in ensembl plants.recent developments in dna sequencing have enabled the large and complex genomes of many crop species to be determined for the first time, even those previously intractable due to their polyploid nature. indeed, over the course of the last 2 years, the genome sequences of several commercially important cereals, notably barley and bread wheat, have become available, as well as those of related wild species. while still incomplete, comparison with other, more completely assembled species suggests ...025432969
conserved cis-regulatory modules in promoters of genes encoding wheat high-molecular-weight glutenin subunits.the concentration and composition of the gliadin and glutenin seed storage proteins (ssps) in wheat flour are the most important determinants of its end-use value. in cereals, the synthesis of ssps is predominantly regulated at the transcriptional level by a complex network involving at least five cis-elements in gene promoters. the high-molecular-weight glutenin subunits (hmw-gs) are encoded by two tightly linked genes located on the long arms of group 1 chromosomes. here, we sequenced and anno ...025429295
transcriptome and allele specificity associated with a 3bl locus for fusarium crown rot resistance in bread wheat.fusarium pathogens cause two major diseases in cereals, fusarium crown rot (fcr) and head blight (fhb). a large-effect locus conferring resistance to fcr disease was previously located to chromosome arm 3bl (designated as qcrs-3b) and several independent sets of near isogenic lines (nils) have been developed for this locus. in this study, five sets of the nils were used to examine transcriptional changes associated with the qcrs-3b locus and to identify genes linked to the resistance locus as a ...025405461
can we use photography to estimate radiation interception by a crop canopy?accuracy of determining radiation interception, and hence radiation use efficiency, depends on the method of measuring photosynthetically active radiation intercepted. methods vary, from expensive instruments such as sunfleck ceptometers to simple methods such as digital photography. however, before universal use of digital photography there is need to determine its reliability and compare it with conventional, but expensive, methods. in a series of experiments at lincoln, new zealand, canopy de ...025348838
identification of kernel proteins associated with the resistance to fusarium head blight in winter wheat (triticum aestivum l.).numerous potential components involved in the resistance to fusarium head blight (fhb) in cereals have been indicated, however, our knowledge regarding this process is still limited and further work is required. two winter wheat (triticum aestivum l.) lines differing in their levels of resistance to fhb were analyzed to identify the most crucial proteins associated with resistance in this species. the presented work involved analysis of protein abundance in the kernel bulks of more resistant and ...025340555
allele frequencies in the vrn-a1, vrn-b1 and vrn-d1 vernalization response and ppd-b1 and ppd-d1 photoperiod sensitivity genes, and their effects on heading in a diverse set of wheat cultivars (triticum aestivum l.).heading of cereals is determined by complex genetic and environmental factors in which genes responsible for vernalization and photoperiod sensitivity play a decisive role. our aim was to use diagnostic molecular markers to determine the main allele types in vrn-a1, vrn-b1, vrn-d1, ppd-b1 and ppd-d1 in a worldwide wheat collection of 683 genotypes and to investigate the effect of these alleles on heading in the field. the dominant vrn-a1, vrn-b1 and vrn-d1 alleles were present at a low frequency ...025076837
high-frequency generation and characterization of intergeneric hybrids and haploids from new wheat-barley crosses.hybrid plants and a high frequency of maternal haploids were obtained using an efficient wheat-barley hybridization system (with new genotype combinations) and confirmed by several cytological and molecular tools. an efficient hybridization system between wheat (triticum aestivum l.) and barley (hordeum vulgare l.) is presented on the basis of three new genotype combinations. a particularly high, 14% frequency of plant regeneration per florets was achieved in the wheat-barley genotype combinatio ...024770442
wheat-aegilops biuncialis amphiploids have efficient photosynthesis and biomass production during osmotic stress.osmotic stress responses of water content, photosynthetic parameters and biomass production were investigated in wheat-aegilops biuncialis amphiploids and in wheat genotypes to clarify whether they can use to improve the drought tolerance of bread wheat. a decrease in the osmotic pressure of the medium resulted in considerable water loss, stomatal closure and a decreased co2 assimilation rate for the wheat genotypes, while the changes in these parameters were moderate for the amphiploids. maxima ...024655386
the shutdown of celiac disease-related gliadin epitopes in bread wheat by rnai provides flours with increased stability and better tolerance to over-mixing.celiac disease is a food-sensitive enteropathy triggered by the ingestion of wheat gluten proteins and related proteins from barley, rye, and some varieties of oat. there are no interventional therapies and the only solution is a lifelong gluten-free diet. the down-regulation of gliadins by rnai provides wheat lines with all the gliadin fractions strongly down-regulated (low-gliadin). the technological properties of doughs prepared from the low-gliadin lines indicated a general weakening effect, ...024633046
variability of non-symbiotic and truncated hemoglobin genes from the genome of cultivated monocots.non-symbiotic (nshb) and truncated (thb) hemoglobins (hbs) have been detected in a variety of land plants. the evolution of land plant nshbs and thbs at the protein level is well documented; however, little is known about the evolution of genes coding for these proteins. for example, the variability of the land plant nshb and thb genes is not known. here, we report the variability of the nshb and thb genes from the genome of the cultivated monocots brachypodium distachyon, hordeum vulgare (barle ...024563718
gluten and wheat intolerance today: are modern wheat strains involved?celiac disease is a food-induced enteropathy resulting from exposure to gluten in genetically predisposed individuals. the non-celiac gluten sensitivity (ncgs) is a less known syndrome whose prevalence is under-estimated. the last decades have seen changes in the clinical presentation of both diseases. one possible explanation is that changes in the gluten-rich cereals themselves were the principal causes. celiac-triggering gluten proteins are indeed expressed to higher levels in modern cereals ...024524657
senescence, nutrient remobilization, and yield in wheat and barley.cereals including wheat and barley are of primary importance to ensure food security for the 21st century. a combination of lab- and field-based approaches has led to a considerably improved understanding of the importance of organ and particularly of whole-plant (monocarpic) senescence for wheat and barley yield and quality. a delicate balance between senescence timing, grain nutrient content, nutrient-use efficiency, and yield needs to be considered to (further) improve cereal varieties for a ...024470467
the erf transcription factor taerf3 promotes tolerance to salt and drought stresses in wheat.salinity and drought are major limiting factors of wheat (triticum aestivum) productivity worldwide. here, we report the function of a wheat erf transcription factor taerf3 in salt and drought responses and the underlying mechanism of taerf3 function. upon treatment with 250 mm nacl or 20% polyethylene glycol (peg), transcript levels of taerf3 were rapidly induced in wheat. using wheat cultivar yangmai 12 as the transformation recipient, four taerf3-overexpressing transgenic lines were generated ...024393105
evaluation of triticale as energy crop in italy.the promotion of renewable energy represents a target of the european 2020 strategy for economical growth and sustainable competitiveness. cereals are considered a promising biomass producing crop in temperate regions of europe to be used for both fuel alcohol and biogas production. among cereals, triticale represents a good candidate for this kind of application, showing a number of advantages such as high grain yield even in marginal environments, tolerance to drought, tolerance to more acid s ...026072589
triticale adaption and competence assessment result in the high lands of ethiopia.triticale is a crop that resulted from the addition of chromosomes of wheat (triticum aestivum ) and rye cereals (secale cereale). the crop came on the market as bread cereal in the 1980s. different varieties were released. triticale is a high yielding crop when compared with tef, wheat and barley, particularly on locations with soil nutrient deficiency. the study was initiated with the question to which extent the growing of triticale crop (triticosecale wittmack) improves food security, and wh ...026072574
interaction of photoperiod and vernalization determines flowering time of brachypodium distachyon.timing of flowering is key to the reproductive success of many plants. in temperate climates, flowering is often coordinated with seasonal environmental cues such as temperature and photoperiod. vernalization is an example of temperature influencing the timing of flowering and is defined as the process by which a prolonged exposure to the cold of winter results in competence to flower during the following spring. in cereals, three genes (vernalization1 [vrn1], vrn2, and flowering locus t [ft]) h ...024357601
temporal variation of mycotoxin producing fungi in norwegian cereals.spring barley is grown on about half of the norwegian cereal area. the rest of the area is equally divided between wheat and oats. most years the domestic production provides 70%-80% of the domestic market for bread wheat. barley and oats are mainly grown for animal feed. during the years 2008-2012, severe epidemics of fusarium head blight have led to increased mycotoxin contamination of cereals. during that period, precipitation was above normal during anthesis and grain maturation. the most im ...027694772
treatment with the herbicide topik induces oxidative stress in cereal leaves.leaf disks as well as intact 7-day-old plants of winter wheat (triticum aestivum l., cv. mironovskaya 808), winter rye (secale cereale l., cv. estafeta tatarstana), and maize (zea mays l., cv. kollektivnyi 172mv), were treated with the aryloxyphenoxypropionate class herbicide topik, concentrate-emulsion (active ingredient is clodinafop-propargyl (cp), 8-800μg/l), and the effects of short-term action (up to 3h) and long-term aftereffect (up to 3days) on physiological and biochemical indices relat ...024238289
occurrence of mycotoxins in spelt and common wheat grain and their products.organic farming does not allow the use of conventional mineral fertilizers and crop protection products. as a result, in our experiments we chose to grow different species of cereals and to see how cereal species affect mycotoxin accumulation. this study describes the occurrence of deoxynivalenol (don), zearalenone (zea) and t-2/ht-2 toxin in a survey of spelt and common wheat and their bran as well as flour. the analysis was conducted using an enzyme-linked immunosorbent assay (elisa) method. t ...024199659
yield stability of hybrids versus lines in wheat, barley, and triticale.we present experimental data for wheat, barley, and triticale suggesting that hybrids manifest on average higher yield stability than inbred lines. yield stability is assumed to be higher for hybrids than for inbred lines, but experimental data proving this hypothesis is scarce for autogamous cereals. we used multi-location grain yield trials and compared the yield stability of hybrids versus lines for wheat (triticum aestivum l.), barley (hordeum vulgare l.), and triticale (×triticosecale wittm ...024162154
comparing sugar components of cereal and pseudocereal flour by gc-ms analysis.gas chromatography with mass spectrometry was used for carrying out a qualitative analysis of the ethanol soluble flour extract of different types of cereals bread wheat and spelt and pseudocereals (amaranth and buckwheat). tmsi (trimethylsilylimidazole) was used as a reagent for the derivatisation of carbohydrates into trimethylsilyl ethers. all samples were first defatted with hexane. (in our earlier investigations, hexane extracts were used for the analysis of fatty acid of lipid components.) ...024128539
reticulate evolution of the rye genome.rye (secale cereale) is closely related to wheat (triticum aestivum) and barley (hordeum vulgare). due to its large genome (~8 gb) and its regional importance, genome analysis of rye has lagged behind other cereals. here, we established a virtual linear gene order model (genome zipper) comprising 22,426 or 72% of the detected set of 31,008 rye genes. this was achieved by high-throughput transcript mapping, chromosome survey sequencing, and integration of conserved synteny information of three se ...024104565
next-generation survey sequencing and the molecular organization of wheat chromosome 6b.common wheat (triticum aestivum l.) is one of the most important cereals in the world. to improve wheat quality and productivity, the genomic sequence of wheat must be determined. the large genome size (∼17 gb/1 c) and the hexaploid status of wheat have hampered the genome sequencing of wheat. however, flow sorting of individual chromosomes has allowed us to purify and separately shotgun-sequence a pair of telocentric chromosomes. here, we describe a result from the survey sequencing of wheat ch ...024086083
impact of marker ascertainment bias on genomic selection accuracy and estimates of genetic diversity.genome-wide molecular markers are often being used to evaluate genetic diversity in germplasm collections and for making genomic selections in breeding programs. to accurately predict phenotypes and assay genetic diversity, molecular markers should assay a representative sample of the polymorphisms in the population under study. ascertainment bias arises when marker data is not obtained from a random sample of the polymorphisms in the population of interest. genotyping-by-sequencing (gbs) is rap ...024040295
coverage and consistency: bioinformatics aspects of the analysis of multirun itraq experiments with wheat leaves.the hexaploid genome of bread wheat (triticum aestivum) is large (17 gb) and repetitive, and this has delayed full sequencing and annotation of the genome, which is a prerequisite for effective quantitative proteomics analysis. aware of these constraints we investigated the most effective approaches for shotgun proteomic analyses of bread wheat that would support large-scale quantitative comparisons using itraq reagents. we used a data set that was generated by two-dimensional lc-ms of itraq lab ...024015675
pattern of iron distribution in maternal and filial tissues in wheat grains with contrasting levels of iron.iron insufficiency is a worldwide problem in human diets. in cereals like wheat, the bran layer of the grains is an important source of iron. however, the dietary availability of iron in wheat flour is limited due to the loss of the iron-rich bran during milling and processing and the presence of anti-nutrients like phytic acid that keep iron strongly chelated in the grain. the present study investigated the localization of iron and phosphorus in grain tissues of wheat genotypes with contrasting ...023918965
tamyb13-1, a r2r3 myb transcription factor, regulates the fructan synthetic pathway and contributes to enhanced fructan accumulation in bread wheat.fructans are the major component of temporary carbon reserve in the stem of temperate cereals, which is used for grain filling. three families of fructosyltransferases are directly involved in fructan synthesis in the vacuole of triticum aestivum. the regulatory network of the fructan synthetic pathway is largely unknown. recently, a sucrose-upregulated wheat myb transcription factor (tamyb13-1) was shown to be capable of activating the promoter activities of sucrose:sucrose 1-fructosyltransfera ...023873993
the effect of day-neutral mutations in barley and wheat on the interaction between photoperiod and vernalization.vernalization-2 (vrn-2) is the major flowering repressor in temperate cereals. it is only expressed under long days in wild-type plants. we used two day-neutral (photoperiod insensitive) mutations that allow rapid flowering in short or long days to investigate the day length control of vrn-2. the barley (hordeum vulgare) early maturity8 (eam8) mutation affects the barley elf3 gene. eam8 mutants disrupt the circadian clock resulting in elevated expression of ppd-h1 and the floral activator hvft1 ...023737074
isolation of a wheat (triticum aestivum l.) mutant in aba 8'-hydroxylase gene: effect of reduced aba catabolism on germination inhibition under field condition.pre-harvest sprouting, the germination of mature seeds on the mother plant under moist condition, is a serious problem in cereals. to investigate the effect of reduced abscisic acid (aba) catabolism on germination in hexaploid wheat (triticum aestivum l.), we cloned the wheat aba 8'-hydroxyase gene which was highly expressed during seed development (taaba8'oh1) and screened for mutations that lead to reduced aba catabolism. in a screen for natural variation, one insertion mutation in exon 5 of t ...023641187
ability of leaf mesophyll to retain potassium correlates with salinity tolerance in wheat and barley.this work investigated the importance of the ability of leaf mesophyll cells to control k(+) flux across the plasma membrane as a trait conferring tissue tolerance mechanism in plants grown under saline conditions. four wheat (triticum aestivum and triticum turgidum) and four barley (hordeum vulgare) genotypes contrasting in their salinity tolerance were grown under glasshouse conditions. seven to 10-day-old leaves were excised, and net k(+) and h(+) fluxes were measured from either epidermal or ...023611560
protective responses induced by herbicide safeners in wheat.safeners are agrochemicals which enhance tolerance to herbicides in cereals including wheat (triticum aestivum l.) by elevating the expression of xenobiotic detoxifying enzymes, such as glutathione transferases (gsts). when wheat plants were spray-treated with three safener chemistries, namely cloquintocet mexyl, mefenpyr diethyl and fenchlorazole ethyl, an apparently identical subset of gsts derived from the tau, phi and lambda classes accumulated in the foliage. treatment with the closely rela ...023564986
the role of thioredoxin h in protein metabolism during wheat (triticum aestivum l.) seed germination.thioredoxin h can regulate the redox environment in the cell and play an important role in the germination of cereals. in the present study, the thioredoxin s antisense transgenic wheat with down-regulation of thioredoxin h was used to study the role of thioredoxin h in protein metabolism during germination of wheat seeds, and to explore the mechanism of the thioredoxin s antisense transgenic wheat seeds having high resistance to pre-harvest sprouting. the qrt-pcr results showed that the express ...023562797
regulation of wheat seed dormancy by after-ripening is mediated by specific transcriptional switches that induce changes in seed hormone metabolism and signaling.treatments that promote dormancy release are often correlated with changes in seed hormone content and/or sensitivity. to understand the molecular mechanisms underlying the role of after-ripening (seed dry storage) in triggering hormone related changes and dormancy decay in wheat (triticum aestivum), temporal expression patterns of genes related to abscisic acid (aba), gibberellin (ga), jasmonate and indole acetic acid (iaa) metabolism and signaling, and levels of the respective hormones were ex ...023437172
natural variation in grain composition of wheat and related cereals.the wheat grain comprises three groups of major components, starch, protein, and cell wall polysaccharides (dietary fiber), and a range of minor components that may confer benefits to human health. detailed analyses of dietary fiber and other bioactive components were carried out under the eu fp6 healthgrain program on 150 bread wheat lines grown on a single site, 50 lines of other wheat species and other cereals grown on the same site, and 23-26 bread wheat lines grown in six environments. prin ...023414336
evidence from principal component analysis for improvement of grain shape- and spikelet morphology-related traits after hexaploid wheat speciation.grain shape and size are involved in the main components of the domestication syndrome in cereals. wheat grain shape has been dramatically altered at each stage of the domestication of tetraploid wheat and through common wheat speciation. to elucidate the evolutionary change of wheat grain shape, principal component (pc) analysis of grain shape-related traits was first conducted using wild and cultivated tetraploid, synthetic hexaploid, and common wheat accessions. the synthetic hexaploid wheat ...023412632
integrating cereal genomics to support innovation in the triticeae.the genomic resources of small grain cereals that include some of the most important crop species such as wheat, barley, and rye are attaining a level of completion that now is contributing to new structural and functional studies as well as refining molecular marker development and mapping strategies for increasing the efficiency of breeding processes. the integration of new efforts to obtain reference sequences in bread wheat and barley, in particular, is accelerating the acquisition and inter ...023161406
an endogenous reference gene of common and durum wheat for detection of genetically modified wheat.to develop a method for detecting gm wheat that may be marketed in the near future, we evaluated the proline-rich protein (prp) gene as an endogenous reference gene of common wheat (triticum aestivum l.) and durum wheat (triticum durum l.). real-time pcr analysis showed that only dna of wheat was amplified and no amplification product was observed for phylogenetically related cereals, indicating that the prp detection system is specific to wheat. the intensities of the amplification products and ...023154759
development of methods to distinguish between durum/common wheat and common wheat in blended flour using pcr.a pcr-based method was developed to distinguish between durum/common wheat and common wheat by leveraging slight differences of dna sequence in starch synthase ii (ss ii) coded on wheat a, b and d genomes. a primer pair, ss ii ex7-u/l, was designed to hybridize with a conserved dna sequence region found in ss ii-a, b and d genes. another primer pair, ss ii-d 1769u/1889l, was constructed to recognize a unique sequence in the ss ii-d gene. the target region of ss ii ex7-u/l with the size of 114 bp ...023154758
[a cytogenetic study of the blue-grain line of the common wheat cultivar saratovskaya 29].the chromosome composition of the blue-grain line i:s29ba of the cultivar saratovskaya 29 was identified by cytological, gish, and microsatellite analyses and c-banding. it was found that common wheat chromosome 4b of the cultivar saratovskaya 29 was substituted with the agropyron elongatum host. chromosome carrying the gene for blue grain (s:s294ag(4b)) during the construction of this nearly isogenic line. the blue-grain line was tested for productivity. the substitution of total chromosome 4b ...023035544
development and validation of an hplc-method for determination of free and bound phenolic acids in cereals after solid-phase extraction.whole cereal grains are a good source of phenolic acids associated with reduced risk of chronic diseases. this paper reports the development and validation of a high-performance liquid chromatography-diode array detection (hplc-dad) method for the determination of phenolic acids in cereals in either free or bound form. extraction of free phenolic acids and clean-up was performed by an optimised solid-phase extraction (spe) protocol on oasis hlb cartridges using aqueous methanol as eluant. the me ...025005991
functional characterization of wheat ent-kaurene(-like) synthases indicates continuing evolution of labdane-related diterpenoid metabolism in the cereals.wheat (triticum aestivum) and rice (oryza sativa) are two of the most agriculturally important cereal crop plants. rice is known to produce numerous diterpenoid natural products that serve as phytoalexins and/or allelochemicals. specifically, these are labdane-related diterpenoids, derived from a characteristic labdadienyl/copalyl diphosphate (cpp), whose biosynthetic relationship to gibberellin biosynthesis is evident from the relevant expanded and functionally diverse family of ent-kaurene syn ...023009879
functional characterization of wheat copalyl diphosphate synthases sheds light on the early evolution of labdane-related diterpenoid metabolism in the cereals.two of the most agriculturally important cereal crop plants are wheat (triticum aestivum) and rice (oryza sativa). rice has been shown to produce a number of diterpenoid natural products as phytoalexins and/or allelochemicals--specifically, labdane-related diterpenoids, whose biosynthesis proceeds via formation of an eponymous labdadienyl/copalyl diphosphate (cpp) intermediate (e.g., the ent-cpp of gibberellin phytohormone biosynthesis). similar to rice, wheat encodes a number of cpp synthases ( ...023009878
a comparison of contents of group a and b trichothecenes and microbial counts in different cereal species.concentrations of trichothecenes and the amounts of microbial biomass were compared in grain of bread wheat, durum wheat, triticale, rye, oat and barley. grain samples came from lines regionalised in poland grown under identical climatic and agricultural conditions in 2007. among the six analysed cereals, the highest mean concentration of toxic metabolites of 151.89 µg/kg was found for grain of triticum durum, whereas the lowest was for barley grain (25.56 µg/kg). the highest contamination with ...024779779
expression of vernalization responsive genes in wheat is associated with histone h3 trimethylation.the transition to flowering in winter wheat requires prolonged exposure to low temperature, a process called vernalization. this process is regulated by a genetic pathway that involves at least three genes, triticum aestivum vernalization 1 (tavrn1), triticum aestivum vernalization 2 (tavrn2) and triticum aestivum flowering locus t-like 1 (taft1). these genes regulate flowering by integrating environmental and developmental cues. to determine whether the expression of these genes is associated w ...022684814
carbon and nitrogen partitioning during the post-anthesis period is conditioned by n fertilisation and sink strength in three cereals.further knowledge of the processes conditioning nitrogen use efficiency (nue) is of great relevance to crop productivity. the aim of this paper was characterise c and n partitioning during grain filling and their implications for nue. cereals such as bread wheat (triticum aestivum l. cv califa sur), triticale (× triticosecale wittmack cv. imperioso) and tritordeum (× tritordeum asch. & graebn line ht 621) were grown under low (ln, 5 mm nh(4) no(3)) and high (hn, 15 mm nh(4)no(3)) n conditions. w ...022672709
genotype-dependent efficiency of endosperm development in culture of selected cereals: histological and ultrastructural studies.the paper reports studies, including histological and ultrastructural analyses, of in vitro cell proliferation and development of immature endosperm tissue isolated from caryopses of triticum aestivum, triticum durum, and triticosecale plants. endosperm isolated at 7-10 days post-anthesis developed well on ms medium supplemented with auxins and/or cytokinins. the efficiency of endosperm response was highly genotype-dependent and best in two winter cultivars of hexaploid species. the pathways of ...022643840
optimization and validation of the reversed-phase high-performance liquid chromatography with fluorescence detection method for the separation of tocopherol and tocotrienol isomers in cereals, employing a novel sorbent material.the separation and determination of tocopherols (ts) and tocotrienols (t3s) by reversed-phase high-performance liquid chromatography with fluorescence detection has been developed and validated after optimization of various chromatographic conditions and other experimental parameters. analytes were separated on a perfectsil target ods-3 (250 × 4.6 mm, 3 μm) column filled with a novel sorbent material of ultrapure silica gel. the separation of ts and t3s was optimized in terms of mobile-phase com ...022324450
a genetic playground for enhancing grain number in cereals.improving the yield stability of cereal crops with a view to bolstering global food security is an important priority. the components of final grain number per plant at harvest are determined by fertile spikes per plant, number of fertile spikelets per spike and number of grains per spikelet. in this review article, we focus on the genetic factors of floral development and inflorescence architecture known to influence grain number and provide a broad overview of genes and genetic pathways that p ...022197176
effect of germination on the phytase activity, phytate and total phosphorus contents of rice (oryza sativa), maize (zea mays), millet (panicum miliaceum), sorghum (sorghum bicolor) and wheat (triticum aestivum).the effect of germination on the level of phytase activity and the contents of phytates and phosphorus of five nigeria grown cereal grains was studied. the cereals screened were rice (oryza sativa), maize (zea mays), millet (panicum miliaceum), sorghum (sorghum bicolor) and wheat (triticum aestivum). phytase activity was high (0.21-0.67 u g(-1)) in all samples. phytate content ranged between 5.6 and 6.2 mg g(-1) while total phosphorus content ranged between 3.3 and 4.3 mg g(-1). during germinati ...023572811
diagnosis and control of cereal viruses in the middle east.middle eastern countries are major consumers of small grain cereals. egypt is the biggest bread wheat producer with 7.4 million tons (mt) in 2007, but at the same time, it had to import 5.9 mt. jordan and israel import almost all the grains they consume. viruses are the major pathogens that impair grain production in the middle east, infecting in some years more than 80% of the crop. they are transmitted in nonpersistent, semipersistent, and persistent manners by insects (aphids, leafhoppers, an ...022094078
genetic control of duration of pre-anthesis phases in wheat (triticum aestivum l.) and relationships to leaf appearance, tillering, and dry matter accumulation.the duration of pre-anthesis developmental phases is of interest in breeding for improved adaptation and yield potential in temperate cereals. yet despite numerous studies on the genetic control of anthesis (flowering) time and floral initiation, little is known about the genetic control of other pre-anthesis phases. furthermore, little is known about the effect that changes in the duration of pre-anthesis phases could have on traits related to leaf appearance and tillering, or dry matter accumu ...021920907
lipid binding interactions of antimicrobial plant seed defence proteins: puroindoline-a and β-purothionin.the indolines and thionins are basic, amphiphilic and cysteine-rich proteins found in cereals; puroindoline-a (pin-a) and β-purothionin (β-pth) are members of these families in wheat (triticum aestivum). pin-a and β-pth have been suggested to play a significant role in seed defence against microbial pathogens, making the interaction of these proteins with model bacterial membranes an area of potential interest. we have examined the binding of these proteins to lipid monolayers composed of 1,2-di ...021869972
molecular, phylogenetic and comparative genomic analysis of the cytokinin oxidase/dehydrogenase gene family in the poaceae.the genomes of cereals such as wheat (triticum aestivum) and barley (hordeum vulgare) are large and therefore problematic for the map-based cloning of agronomicaly important traits. however, comparative approaches within the poaceae permit transfer of molecular knowledge between species, despite their divergence from a common ancestor sixty million years ago. the finding that null variants of the rice gene cytokinin oxidase/dehydrogenase 2 (osckx2) result in large yield increases provides an opp ...021838715
two wheat (triticum aestivum) pathogenesis-related 10 (pr-10) transcripts with distinct patterns of abundance in different organs.pr-10 genes encode small, acidic, intracellular proteins that respond to abiotic and biotic stimuli. transgenic expression of pr-10 genes has been shown to enhance early seedling growth of dicots in saline environments. to identify candidate pr-10 genes in cereals for increasing stress tolerance, we conducted phylogenetic analyses and real-time polymerase chain reaction of representatives of the two major clades of putative pr-10 genes in wheat. we observed that the abundance of bq752893 was gen ...021818707
genetic analysis of carbon isotope discrimination and its relation to yield in a wheat doubled haploid population.carbon isotope discrimination (δ(13)c) is considered a useful indicator for indirect selection of grain yield (gy) in cereals. therefore, it is important to evaluate the genetic variation in δ(13)c and its relationship with gy. a doubled haploid (dh) population derived from a cross of two common wheat varieties, hanxuan 10 (h10) and lumai 14 (l14), was phenotyped for δ(13)c in the flag leaf, gy and yield associated traits in two trials contrasted by water availability, specifically, rain-fed and ...021762383
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