Publications

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the occurrence of apiose in lemna (duckweed) and other angiosperms.1. the branched-chain pentose apiose reacts with the benzidine-trichloroacetic acid reagent on paper chromatograms to give a yellow spot with.an intense white fluorescence in ultraviolet light; on chromatograms developed with butanol-acetic acid-water this spot lies between fucose and rhamnose. 2. examination of paper chromatograms of hydrolysates of whole plant material has shown the presence of a substance with these properties in a wide variety of species. 3. among the plants examined two mem ...196514340070
[studies on the role of silicic acid in the development of higher plants].germanium acid, a specific inhibitor of the silicic acid metabolism in diatoms, inhibits the growth of sinapis alba, lemna minor, wolffia arrhiza, nicotiana tabacum, tradescantia spec, zinnia elegans, and secale cereale when applied in the same concentrations as those used in the case of diatoms (15-75 μg geo2/ml medium). the growth of aspergillus niger, phycomyces blakesleanus, escherichia coli k 12, euglena gracilis and pandorina morum is not influenced by these and higher concentrations of ge ...196724549376
protein synthesis in sterile chloroplasts from lemna minor l. 19694935421
[assimilation of nitrate in the dark by lemna minor l].1. lemna minor is able to reduce nitrate in the dark when the nutrient medium contains yeast extract and glucose in addition to minerals salts. 2. when organic n is offered as a source of n in addition to nitrate, the former is assimilated preferentially, but both assimilation processes take place simultaneously. 3. assimilation of nitrate in the dark under ecological conditions can be excluded.197024500129
phytochrome-mediated growth responses in green and etiolated lemna minor.the growth of lemna minor in darkness is log-linear, at a much reduced rate compared to growth in white or red light. this rate of frond production in darkness is stimulated by kinetin, yeast extract, and thiamine either in green plants transferred directly from the light or in plants which had been grown in the dark for 54 days. (fig. 1).the magnitude of the stimulation of frond production by interruption of darkgrowth with red light (fig. 2) is smaller in green than in etiolated plants, and is ...197224474160
characterization of particulate d-apiosyl-and d-xylosyltransferase from lemna minor. 197720844
effects of four agricultural pesticides on daphnia pulex, lemna minor, and potamogeton pectinatus. 19854074929
mineralization of surfactants by microbiota of aquatic plants.the biodegradation of linear alkylbenzene sulfonate (las) and linear alcohol ethoxylate (lae) by the microbiota associated with duckweed (lemna minor) and the roots of cattail (typha latifolia) was investigated. plants were obtained from a pristine pond and a pond receiving wastewater from a rural laundromat. cattail roots and duckweed plants were incubated in vessels containing sterile water amended with [c]las, [c]lae, or c-labeled mixed amino acids (maa). evolution of co(2) was determined ove ...198916347999
fate and effects of salicylic acid compounds in freshwater systems.the comparative fates and effects of salicylic acid (sa) and na salicylate in algae (scenedesmus subspicatus, monoraphidium minutum), in lemna minor, and in daphnia magna were examined. test methods were principally based on the oecd testing guidelines with modifications in the procedures. the influence of fulvic acid (fa) on bioconcentration and on toxic effects was studied. fa addition significantly reduced the bioavailability of sa in l. minor and the algae species. sa was more toxic to lemna ...19892743919
the maximization of the productivity of aquatic plants for use in controlled ecological life support systems (celss).lemna minor (common duckweed) and a wolffia sp. were grown in submerged growth systems. submerged growth increased the productivity unit volume (p/uv) of the organisms and may allow these plants to be used in a controlled ecological life support system (celss).198911541161
distribution and abundance of larval coquillettidia perturbans in a florida freshwater marsh.all instars of larval coquillettidia perturbans were found in the same habitats, but early instar larvae were more aggregated than later instars. larvae were most numerous in areas dominated by arrow-arum (peltandra virginica) and maidencane (panicum hemitomon), less so in areas dominated by sedges (carex spp.) and miscellaneous mixed vegetation, and least abundant in pickerelweed (pontederia cordata) areas. larvae were uncommon in open water or in areas dominated by small floating plants such a ...19901977879
survival of toxigenic vibrio cholerae o1 with a common duckweed, lemna minor, in artificial aquatic ecosystems.cholera epidemics occur twice a year in bangladesh. during epidemics, vibrio cholerae o1 are isolated from patients, as well as from the surface water, but the bacteria disappear during inter-epidemic periods. their reservoirs or sites of survival and multiplication during inter-epidemic period are still unknown. the present survival study in the laboratory explored the role of an aquatic plant, lemna minor (duckweed), as a possible reservoir. l. minor was added to sea-salt solution at ph 8.5, c ...19902260179
acceleration of catalase and peroxidase activities in lemna minor l. and allium cepa l. in response to low levels of aquatic mercury.the purpose of this study was to assess certain physiological responses of lemna minor l. (duckweed) and allium cepa l. (onion) to aquatic mercury at low concentrations. following a 96-h exposure of plants to nutrient medium contaminated with known levels of mercuric chloride (hgcl(2)), 0.001 to 4 mg litre(-1) (0.0007 to 2.95 mg hg litre(-1)) or methyl mercuric chloride (mehgcl(2)), 0.0001 to 0.1 mg litre(-1) (0.0007 to 0.07 mg hg litre(-1)), the physiological endpoints measured were the growth ...199115092159
[evaluation of biological toxicity of livestock fecal deordoring microbial agents].eight commercial livestock microbial agents were selected for the evaluation of biological toxicity. the general physical, chemical and microbial analyses of these microbial agents were performed and the acute toxicity of mice, aquatic living organisms and the germinated inhibition of vegetable seeds by these microbial agents were also estimated for the evaluation of biological toxicity. the results have shown that all livestock microbial agents have no pathogenic salmonella and staphylococcus a ...19921342642
fate of a volatile chlorinated solvent in indoor aquatic microcosms: sublethal and static exposure to [14c]dichloromethane. groupe pour l'etude du devenir de xénobiotiques dans l'environnement (gedexe).the goal of this work was to study the fate of dichloromethane in indoor aquatic microcosms after a sublethal and static exposure to simulate the accidental contamination of a lenitic ecosystem such as littoral lake zone or a pond. this kind of ecosystem is characterized by high productive capacity and rich biocoenose, and are usually first affected by acute or chronic pollution. microcosms containing immersed bryophytes (fontinalis antipyretica), macrophytes (lemna minor, groenlendia densa, elo ...19947523069
effect of cations on the elution of pectic polysaccharides from anion-exchange resins.conditions for quantitatively eluting six plant pectic polysaccharides from diethylaminoethyl (deae)-columns were established. cations, surprisingly, greatly affected the elution of pectic polysaccharides from these anion-exchange columns. quantitative elution of apple pectic acid was achieved when columns of deae-sephadex a-25 and deae-trisacryl plus-m were developed with 0.5 m nh4cl, 0.5 m licl, or 0.5 m cscl in buffer. in contrast, up to 1 m nacl and kcl in buffer only eluted 6.3 to 54% of th ...19958572266
covalent dimerization of ribulose bisphosphate carboxylase subunits by uv radiation.the effect of uv radiation (uv-a, uv-b and uv-c) on ribulose bisphosphate carboxylase from a variety of plant species was examined. the exposition of plant leaves or the pure enzyme to uv radiation produced a uv-dependent accumulation of a +5 kda polypeptide (p65). different approaches were utilized to elucidate the origin and structure of p65: electrophoretic and fluorographic analyses of 35s-labelled ribulose bisphosphate carboxylase exposed to uv radiation and immunological experiments using ...19968761476
removal of heavy metals by two free floating aquatic macrophytes.a concentration-dependent decrease in biomass, protein, rna, dna, and nutrient (no3(-) and po4(3-)) uptake of lemna minor and azolla pinnata by cr, ni, and zn was detected. cr was found to exert maximum toxicity followed by ni and zn. metal uptake was dependent on time and concentration of metal in the external medium. both the macrophytes, however, showed preference for zn followed by ni and cr. the uptake kinetics also revealed a low vmax and high km for cr. l. minor was more efficient in accu ...19968988809
toxicity of tributyltin to lemna minor l. and azolla filiculoides lamk. 19989470996
organophosphorus acid anhydrolase in slime mold, duckweed and mung bean: a continuing search for a physiological role and a natural substrate.recently, and for the first time, a diisopropylphosphorofluoridate (dfp)-hydrolyzing enzyme, i.e. an organophosphorus acid anhydrolase (opaa), has been reported in a plant-source. based on this and other suggestive evidence, the ability of three plant sources and a protist to hydrolyze dfp and 1,2,2-trimethylpropyl methylphosphonofluoridate (soman) were tested, and the effects of mn2+ and ethylenediamine tetraacetate (edta) on this activity. the plants are duckweed (lemna minor), giant duckweed ...199910421476
assessment of toxic interactions of heavy metals in binary mixtures: a statistical approachtoxicity of zinc, copper, cobalt, and chromium ions and their binary interactions were studied at varying test levels by using a battery of two tests, microtox and duckweed with vibrio fisheri and lemna minor as test organisms, respectively. the type of toxic interaction at each test combination was assessed by a statistical approach based on testing the null hypothesis of "additive toxicity" at 95% confidence level. the interactions were called "antagonistic," "additive," or "synergistic" in ac ...199910227854
phytotoxins from the leaves of laggera decurrens.upon biological screening of a series of african medicinal plants, substantial phytotoxic activity was found in the leaves of laggera decurrens (vahl.) hepper & wood (asteraceae), using a lemna minor bioassay. bioassay-guided fractionation of the leaves led to the isolation of two physiologically active compounds: 3-hydroxythymoquinone and 5-acetoxy-2-hydroxythymol, causing death of lemna minor in the 25-100 microm range. symptoms were a rapidly developing chlorosis, followed by necrosis of fron ...199910552505
new steroidal alkaloids from the roots of buxus sempervirensphytochemical studies on an etoh-soluble extract of the roots of buxus sempervirens of turkish origin have resulted in the isolation of three new steroidal alkaloids, (+)-semperviraminol (1), (+)-buxamine f (2), and (+)-17-oxocycloprotobuxine (3), along with two known steroidal alkaloids, (+)-buxoxybenzamine (4) and (+)-buxapapillinine (5). the structures of 1-3 were elucidated with the aid of spectroscopic studies. compounds 4 and 5 exhibited phytotoxic activity against lemna minor.199910346940
effects of dietary copper on the australian freshwater crayfish cherax destructor.little information is available on the effects of metals in the diet of parastacids, despite the importance of the freshwater crayfish industry worldwide. there have been no published studies on the effect of either dissolved or dietary copper on the australian freshwater crayfish cherax destructor. herein is reported a study on the bioaccumulation of copper through a simple food chain. the copper was first absorbed by the floating aquatic macrophyte lemna minor to an average concentration of 74 ...200010806002
toxigenic strains of fusarium moniliforme and fusarium proliferatum isolated from dairy cattle feed produce fumonisins, moniliformin and a new c21h38n2o6 metabolite phytotoxic to lemna minor l.corn samples suspected of causing refusal-to-eat syndrome in dairy cattle were examined mycologically. fusarium moniliforme (14 isolates) and f. proliferatum (12 isolates) were the predominant fungi present. these isolates were tested for mycotoxin production on rice at 25 degrees c. each strain of f. moniliforme produced fumonisin b1 (fb1: 378-15,600 ppm) and fumonisin b2 (fb2: 2-1050 ppm). each strain of f. proliferatum produced moniliformin (45-16,000 ppm), fb1 (27-6140 ppm), and fb2 (5-1550 ...200010868338
adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes.adenosine 5'-phosphosulfate (aps) sulfotransferase and aps reductase have been described as key enzymes of assimilatory sulfate reduction of plants catalyzing the reduction of aps to bound and free sulfite, respectively. aps sulfotransferase was purified to homogeneity from lemna minor and compared with aps reductase previously obtained by functional complementation of a mutant strain of escherichia coli with an arabidopsis thaliana cdna library. aps sulfotransferase was a homodimer with a monom ...200010625629
influence of triphenyllead chloride on biological and model membranes.the physiological and hemolytic toxicities of triphenyllead chloride (tphl) as well as its modifying influence on model lipid membranes were studied. the experiments allowed the determination of tphl concentrations causing 50% inhibition of growth of spirodela oligorrhiza, lemna minor and salvinia natans (ec50), 100% hemolysis of pig erythrocytes (c100) and destabilization of planar lipid membranes (cc). also, fluidity of erythrocyte ghosts was measured by fluorescence technique and osmotic sens ...200011098828
ecotoxicological experiences on lemna minor test system.are plants generally less sensitive to toxicity than aquatic animals as indicated by some authors (1)? the use of phytotoxicity (higher plant) tests as a part of ecotoxicology is relatively underdeveloped. the objective of this study was to compare the results of the oecd lemna growth inhibition test with other mainly used test systems. three toxicants were tested applying the alga growth inhibition test (seleneastrum capricornutum), the daphnia immobilization test (daphnia magna) and the microt ...200010943492
effect of aquatic macrophytes on the quantity of bacterioplankton.the authors investigated the effect of aquatic macrophytes on the quantity of bacterioplankton. among the 7 species of aquatic macrophytes the most pronounced effect on bacterioplankton is exerted by potamogeton crispus, causing a decrease in its quantity to 53.28%. polygonum amphibium stimulates the growth of bacteria to 121.96%. under the influence of macrophytes the spherical to cylindrical ratio changes from 1:1.31 (lemna minor) to 1:2.07 (potamogeton crispus).200011712444
plant adenosine 5'-phosphosulfate reductase is a novel iron-sulfur protein.adenosine 5'-phosphosulfate reductase (apr) catalyzes the two-electron reduction of adenosine 5'-phosphosulfate to sulfite and amp, which represents the key step of sulfate assimilation in higher plants. recombinant aprs from both lemna minor and arabidopsis thaliana were overexpressed in escherichia coli and isolated as yellow-brown proteins. uv-visible spectra of these recombinant proteins indicated the presence of iron-sulfur centers, whereas flavin was absent. this result was confirmed by qu ...200111553635
effects of procymidone, fludioxonil and pyrimethanil on two non-target aquatic plants.procymidone, fludioxonil, and pyrimethanil are widely used to control the pathogenic fungus botrytis cinerea in champagne's vineyards. these fungicides may end up in surface waters and present potential risks for aquatic vascular plants and algae. therefore, their toxicity was evaluated on lemna minor and scenedesmus acutus in six-day or 48-h tests, respectively. based on growth and chlorophyll (chl) content of l. minor and s. acutus cultures, the results showed that the alga was the most sensit ...200111459152
viability of water weeds and microalga in pulp and paper mill wastewater.water weeds azolla pinnata and lemna minor and the chlorophycean alga scendesmus bijugatus were grown in a paper mill wastewater. the objective of the study was to assess the viability of these species upon exposure to such effluents and to assess their potential in the improvement of effluent quality. the wastewater was analysed before and after the treatment with these species. the growth of the alga and the hydrophytes was also measured. the algal assay revealed that the clear effluent will p ...200112395506
xenobiotics removal from polluted water by a multifunctional constructed wetland.removal efficiencies on xenobiotics from polluted water in a twin-shaped constructed wetland consisting of a vertical flow chamber with the crop plant colocasia esculenta l. schott and a reverse vertical flow one with ischaemum aristatum var. glaucum honda, were assessed by chemical analysis and bioassays. after a four-month period of application, removal efficiencies of the applied pesticides parathion and omethoate were 100% with no detectable parathion and omethoate in the effluent. for the a ...200212152743
a chlorophyll a fluorescence-based lemna minor bioassay to monitor microbial degradation of nanomolar to micromolar concentrations of linuron.a plant-microbial bioassay, based on the aquatic macrophyte lemna minor l. (duckweed), was used to monitor biodegradation of nano- and micromolar concentrations of the phenylurea herbicide linuron. after 7 days of exposure to linuron, log-logistic-based dose-response analysis revealed significant growth inhibition on the total frond area of l. minor when linuron concentrations > or = 80 nm were added to the bioassay. a plant-protective effect was obtained for all concentrations > 80 nm by inocul ...200212071978
lanthanide concentrations in freshwater plants and molluscs, related to those in surface water, pore water and sediment. a case study in the netherlands.industrial emissions of lanthanides to aquatic ecosystems increase, but knowledge of the environmental fate of these metals is limited. here we focus attention upon the distribution of lanthanides in freshwater ecosystems, describing lanthanide partitioning between sediment, water and biota. since lanthanides are often used as oxidation-state analogues for actinides, their distribution can reflect long-term behaviour of the radioactive transuranics. concentrations of all 14 naturally occurring l ...200211887873
new triterpenoidal alkaloids from buxus sempervirens.phytochemical studies on the ethanolic extract of the roots of buxus sempervirens of turkish origin have resulted in the isolation of two new triterpenoidal alkaloids, (+)-16a, 31-diacetylbuxadine (1), (-)-nb-demethylcyclomikuranine (2) along with three known natural products, (-)-cyclomikuranine (3), (-)-cyclobuxophylline-k (4) and (+)-buxaquamarine (5) isolated for the first time from this species of genus buxus. the structures of these new natural products were established on the basis of ext ...200211926538
photosynthetic responses of lemna minor exposed to xenobiotics, copper, and their combinations.the effects on the photosynthetic process of copper and pesticides, used in vineyards, and their combinations, were investigated by measuring different chlorophyll fluorescence parameters in lemna minor. cu and flumioxazin had a severe impact on duckweed since a decrease in their photosynthetic capacity was detected after 24h of exposure to 200 and 1 microg.l(-1), respectively. however, fungicides used to control botrytis cinerea (procymidone, pyrimethanil, and fludioxonil) seem to have no marke ...200212485590
bioaccumulation of 65zn2+ ions by some hydrophytic plants.this paper deals with a study on the retention of radioactive ions 65zn2+ from diluted waste solutions by the hydrophytic plants lemna minor, elodea canadensis, pistia stratiotes and riccia fluitans. the bioaccumulation degree decreases in the following order: riccia fluitans approximately pistia stratiotes > or = lemna minor > elodea canadensis the possibility to improve the bioaccumulation degree varying the temperature and the solution ph, in the absence of other cations, has been studied. th ...200212219990
endophenazines a-d, new phenazine antibiotics from the arthropod associated endosymbiont streptomyces anulatus. i. taxonomy, fermentation, isolation and biological activities.four new members of the phenazine family, endophenazines a-d, and the already known phenazine-1-carboxylic acid (tubermycin b) were detected in the culture broth of various endosymbiotic streptomyces anulatus strains by chemical screening in a combination of tlc-staining reagents and hplc-diode array analysis. the endosymbiotic strains were isolated from four different arthropod hosts at various sites. the new phenazine compounds showed antimicrobial activities against gram-positive bacteria and ...200212458768
aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects.to evaluate the ecotoxicological potential of ten prescription drugs against aquatic organisms from different taxonornical classes, a set of biotests were performed using the cladoceran daphnia magna, the chlorophyte desmodesmus subspicatus and the macrophyte lemna minor. endpoints were immobilisation for daphnia and inhibition of the average growth rate for desmodesmus and lemna. for most of the substances, toxicities were moderate, with ec(50)s in the range from 10 to 100 mgl(-1) or even far a ...200312691712
toxicity of copper in sewage sludge.sewage sludge is a source of organic matter and nutrients, but a major obstacle for its recycling is that the municipal wastewater sludge has low but significant levels of contaminants. this investigation, on the acute toxicity of copper in sewage sludge, was conducted with three organisms, daphnia magna, lemna minor and raphanus sativus (seeds). the toxicity of the leakage water from sewage sludge spiked with cuso(4) was studied for 64 days. the toxicity increased during the first 8-16 days and ...200312605925
identification of streptomyces coelicolor proteins that are differentially expressed in the presence of plant material.streptomyces coelicolor and lemna minor were used as a model to study the modulation of bacterial gene expression during plant-streptomycete interactions. s. coelicolor was grown in minimal medium with and without l. minor fronds. bacterial proteomes were analyzed by two-dimensional gel electrophoresis, and a comparison of the two culture conditions resulted in identification of 31 proteins that were induced or repressed by the presence of plant material. one-half of these proteins were identifi ...200312676660
gastroprotective effect of lemnan, a pectic polysaccharide from lemna minor l. 200312940141
ecotoxicogenomics: the challenge of integrating genomics into aquatic and terrestrial ecotoxicology.rapid progress in the field of genomics (the study of how an individual's entire genetic make-up, the genome, translates into biological functions) is beginning to provide tools that may assist our understanding of how chemicals can impact on human and ecosystem health. in many ways, if scientific and regulatory efforts in the 20th century have sought to establish which chemicals cause damage to ecosystems, then the challenge in ecotoxicology for the 21st century is to understand the mechanisms ...200415003699
effects of erythromycin, tetracycline and ibuprofen on the growth of synechocystis sp. and lemna minor.pharmaceutically active substances have recently been recognised as an emerging environmental problem. human and veterinarian therapeutic agents can contaminate aquatic ecosystems via sewage discharges (human and animal excretion), improper disposal or industrial waste. very little is known on the effects of pharmaceutical pollutants on aquatic photosynthetic organisms. in this study the effects of erythromycin, tetracycline and ibuprofen on the growth of the cyanobacterium synechocystis sp. pcc ...200415084414
heavy metal levels and esterase variations between metal-exposed and unexposed duckweed lemna minor: field and laboratory studies.environmental homogeneity is being continuously disturbed and affected by artificially introduced loads of chemical toxicants that also include heavy metals. the tiljala wetlands of the eastern fringe of calcutta, west bengal (india) are a virtual sink for the deposition of urban and industrial wastes that get admixed with the aquatic environment. we have selected lemna minor (duckweed), as a representative of the biota surviving therein for the present study. concentrations of lead, cadmium, ch ...200415120200
assessment of the toxicity of triasulfuron and its photoproducts using aquatic organisms.the toxicological effects of the sulfonylurea herbicide triasulfuron and its photoproducts were assessed on four aquatic organisms. toxicity varied with tested organism and with triasulfuron irradiation time. triasulfuron and its photoproducts had no significant effects on the crustacean (cladocera) daphnia magna (causing 50% effective concentration [ec50] [48 h] = 49 +/- 1 mg/l) and the marine bacteria vibrio fischeri (ec50 [30 min] > 100 mg/l). in contrast, primary producers (the duckweed lemn ...200415648757
evaluation of heat shock protein 70 as a biomarker of environmental stress in fucus serratus and lemna minor.heat shock proteins (hsps) are known to be induced in response to short-term stress. the present study aimed to evaluate the potential of hsp70 as a biomarker of stress produced by increased temperature, osmotic pressure, and exposure to cadmium and sodium chloride in marine macroalgae and fresh water plant species. an indirect competitive enzyme-linked immunosorbent assay (ic-elisa) was developed with a working range of 0.025-10 microg ml(-1) using a monoclonal antibody raised against purified ...200415370872
use of freshwater algae and duckweeds for phytotoxicity testing.the toxicity of contaminated water of different origins and chemicals [cr(iii), pb(ii), cu(ii), cd(ii), pyrene] were tested using four test species: the alga selenastrum capricornutum (new name raphidocelis subcapitata), the duckweed lemna minor, and the crustaceans thamnocephalus platyurus and daphnia magna. on the basis of the results obtained, the sensitivity of plant species and problems concerning the interpretation of the phytotoxicity data are discussed. the data indicated that the sensit ...200415269918
comparison of different physiological parameter responses in lemna minor and scenedesmus obliquus exposed to herbicide flumioxazin.the sensitivity of different physiological parameters in scenedesmus obliquus and lemna minor exposed to herbicide (flumioxazin) was investigated to indicate the most convenient and sensitive parameter. to assess toxicity of flumioxazin, we used a panel of biomarkers: pigment contents, chlorophyll fluorescence parameters and antioxidative enzyme activities. algae and duckweed were exposed to 48-h ic50 for growth rate. in l. minor, the sensitivity of the parameters was as follows: qn > oxygen emm ...200415234090
sensitivity of aquatic plants to the herbicide metsulfuron-methyl.the sensitivity of 12 aquatic plant species to the herbicide metsulfuron-methyl was tested in microcosm experiments under two growth conditions. as reference species, barley (hordeum vulgare) and oil-seed rape (brassica napus) were grown with their roots submerged in the microcosms. two response variables were chosen: relative growth rate and specific leaf area (sla). sla was the most sensitive response variable, with 11 of the 12 aquatic species responding to the herbicide. ec(50) values varied ...200414759661
initial risk assessment for three beta-blockers found in the aquatic environment.daphnia magna, desmodesmus subspicatus and lemna minor were used to determine the ecotoxicity of beta-blockers. propranolol was the most toxic substance, with ec50s of 7.7 mg l-1 in the daphnia test and 0.73 mg l-1 in the algal test. the toxicity of atenolol and metoprolol in the daphnia test was very low, with ec50s of 313 and 438 mg l-1, respectively. in the algal test, metoprolol (ec50: 7.9 mg l-1) was more toxic than atenolol (ec50: 620 mg l-1). lemna minor was the least sensitive species. a ...200515722091
toxicity of fluoroquinolone antibiotics to aquatic organisms.toxicity tests were performed with seven fluoroquinolone antibiotics, ciprofloxacin, lomefloxacin, ofloxacin, levofloxacin, clinafloxacin, enrofloxacin, and flumequine, on five aquatic organisms. overall toxicity values ranged from 7.9 to 23,000 microg/l. the cyanobacterium microcystis aeruginosa was the most sensitive organism (5-d growth and reproduction, effective concentrations [ec50s] ranging from 7.9 to 1,960 microg/l and a median of 49 microg/l), followed by duckweed (lemna minor, 7-d rep ...200515720004
chemical and ecotoxicological assessment of polycyclic aromatic hydrocarbon--contaminated sediments of the niger delta, southern nigeria.the extent of environmental contamination and sources of polycyclic aromatic hydrocarbons (pahs) compounds to sediments of the niger delta, nigeria were assessed using combined chemical analysis and toxicity bioassay techniques. concentrations of two- to six-ring pahs of molecular mass 128-278 and toxicity to vibrio fischeri and lemna minor are considered in this investigation. levels of the sum of the 16 usepa priority pollutant pahs varied from 20.7 to 72.1 ng/g dry weight. sediment pah levels ...200515752497
phytoremediation of effluents from aluminum smelters: a study of al retention in mesocosms containing aquatic plants.four mesocosms were exposed to circumneutral and aluminum (al)-rich wastewater during two successive summers (2000, 2001). the goals of the study were to measure the bioaccumulation of dissolved al by the aquatic plants typha latifolia, lemna minor, nuphar variegatum and potamogeton epihydrus, and to evaluate their importance in the retention of al by the mesocosms. in 2000, inlet concentrations of total monomeric al were reduced by 56% and 29% at the arvida and laterrière mesocosms, respectivel ...200515950256
assessment of contaminated sediments with an indoor freshwater/sediment microcosm assay.this study was conducted to assess the feasibility of using a 2-l, indoor microcosm assay to evaluate five contaminated sediments (a, b, c, d, and e). toxic potential was deduced in the light of general contamination of sediments, pollutant partitioning in microcosms, and biological responses of species (pseudokirchneriella subcapitata, lemna minor, daphnia magna, hyalella azteca, chironomus riparius): e > a > b > c > d. sediments mainly were contaminated by metals (lead and zinc). organic pollu ...200516193752
comparison of different biological methods for the assessment of ecotoxicological risks.to test the effects of heavy metals in river water, we compared the sensitivity of seven different biological test methods, using bacteria (vibrio fischeri), human fl cells, protozoans (paramecium spp.), nematodes (rhabditis oxycerca), aquatic plants (lemna minor), and fishes (leuciscus idus melanotus). as test substance we used a representative mixture of 3.0 microg/l as, 2.5 microg/l pb, 0.8 microg/l cd, 1.7 microg/l cr, 3.9 microg/l cu, 6.7 microg/l ni, 0.4 microg/l hg, and 23.0 microg/l zn, ...200616459144
glycan optimization of a human monoclonal antibody in the aquatic plant lemna minor.n-glycosylation is critical to the function of monoclonal antibodies (mabs) and distinguishes various systems used for their production. we expressed human mabs in the small aquatic plant lemna minor, which offers several advantages for manufacturing therapeutic proteins free of zoonotic pathogens. glycosylation of a mab against human cd30 was optimized by co-expressing the heavy and light chains of the mab with an rna interference construct targeting expression of the endogenous alpha-1,3-fucos ...200617128273
[effects of different concentration copper on pigment content and antioxidase system of spirodela polyrrhiza and lemna minor].the study with water culture showed that when treated with 0.056 mg cu x l(-1), the chlorophyll a, b and (a + b) contents in spirodela polyrrhiza and lemna minor were increased by 11%, 46% and 22%, and 8%, 15% and 11%, while under 0.15 to approximately 5.6 mg cu x l(-1) the contents were decreased by 63%, 62% and 63%, and 46%, 45% and 45%, respectively, compared with no copper added. the malondialdehyde (mda) content in s. polyrrhiza was 2.75 times higher than that in l. minor averagely. the sup ...200616724751
activity of desulfitobacterium sp. strain viet1 demonstrates bioavailability of 2,4-dichlorophenol previously sequestered by the aquatic plant lemna minor.aquatic plants take up and sequester organic contaminants such as chlorophenols through incorporation in cell wall materials and storage in vacuoles. the ultimate fate of plant-sequestered chlorophenols, however, remains unclear. this research investigated 2,4-dichlorophenol (2,4-dcp) sequestration by the aquatic plant lemna minor and evaluated contaminant release and bioavailability after plant death and cellular disruption. 14c-labeled 2,4-dcp was used to establish that contaminant removed fro ...200616468399
is prochloraz a potent synergist across aquatic species? a study on bacteria, daphnia, algae and higher plants.fungicides inhibiting the biosynthesis of ergostrol, such as the triazoles and imidazoles, have been shown to enhance the effect of insecticides on birds, mammals and invertebrates in the terrestrial environment. the synergy is proposed to be due to an effect on p450 monooxygenase enzymes active in pesticide metabolism in these organisms. fungicides often enter the aquatic environment jointly with other pesticides. it is therefore possible that they could act as synergists also in the aquatic en ...200616672166
adjuvant effect of lemnan, pectic polysaccharide of callus culture of lemna minor l. at oral administration.a pectic polysaccharide, lemnan lmc, was extracted from the callus of duckweed lemna minor l. and was tested for adjuvant properties at oral administration with protein antigen. mice were orally immunized thrice with weekly interval with free hen's egg lysozyme or lysozyme with lmc. lemnan lmc was shown to increase delayed type hypersensitivity and serum antilysozyme igg responses. lmc was established to increase levels of both serum igg1 and igg2a subclasses. the concentration of malondialdehyd ...200616684673
characterisation of the oral adjuvant effect of lemnan, a pectic polysaccharide of lemna minor l.lemnan lm, apiogalacturonanic pectin of duckweed lemna minor l. was tested for adjuvant properties following oral administration with protein antigen. male swiss mice were orally immunized thrice with weekly intervals with free ova or ova with lemnan (lm). lemnan lm was shown to increase delayed type hypersensitivity (dth) and serum anti ova igg responses. lm was established to increase levels of both serum igg1 and igg2a subclasses, intestinal iga and failed to elevate levels of serum ige. lemn ...200616725237
simultaneous heavy metal removal mechanism by dead macrophytes.the use of dead, dried aquatic plants, for water removal of metals derived from industrial activities as a simple biosorbent material has been increasing in the last years. the mechanism of simultaneous metal removal (cd2+, ni2+, cu2+, zn2+ and pb2+) by 3 macrophytes biomass (spirodela intermedia, lemna minor and pistia stratiotes) was investigated. l. minor biomass presented the highest mean removal percentage and p. stratiotes the lowest for all metals tested. pb2+ and cd2+ were more efficient ...200615990152
sensitivity of ecotoxicological tests in analysis of superfund sites.during the analysis of environmental risk posed by hazardous waste disposal sites, ecological impact on whole ecosystems should be assessed. it requires a complex testing scheme where surrogate species represent key elements of the ecosystem. however, different organisms are exposed to a differing degree, also, their sensitivity to the same contaminant may vary. a possible way to determine which test reflects most the actual toxic conditions, correlation can be calculated between the measured ec ...200616841472
biochemical responses of two typical duckweeds exposed to dibutyl phthalate.effect of dibutyl phthalate (dbp) on physiologic and biochemical characteristics of two species of duckweed (spirodela polyrhiza and lemna minor) from tai lake in china was investigated. the results indicated that different degree of poisoning symptoms appeared on duckweeds after 7 days' exposure to different concentrations of dbp. with the increase of dbp concentration, content of chlorophyll and soluble protein decreased sharply, and activity of anti-oxidant enzyme systems including catalase ( ...200616835115
effect of lemnan, pectin from lemna minor l., and its fragments on inflammatory reaction.an effect of apiogalacturonanic pectin of duckweed lemna minor l. (lemnan lm) was studied on the inflammatory response to ovalbumin injected intradermally into the footpad of control and ovalbumin-fed mice. lemnan lm (1-2 mg per mouse) was found to enhance by as much as 50-60% the footpad swelling in control mice. oral administration of ovalbumin was shown to result in sensitization that increased inflammation. ovalbumin admixed with lemnan was found to increase by two-fold footpad edema in comp ...200616619370
toxicity of industrial wastewater to the aquatic plant lemna minor l.toxicity evaluation of industrial wastewater has been done by using bioassay system of an aquatic plant lemna minor at different time intervals. growth inhibition was measured as reduction in fresh and dry weight in industrial wastewater and sewage water, exposed l. minor plants. results of fresh and dry weight indicate significant decrease in industrial wastewater and sewage wastewater during the different seasons of the year. at 72 and 96 hr of industrial wastewater exposure, decrease in chlor ...200617436529
regulation of sulfate assimilation in arabidopsis and beyond.sulfate assimilation is a pathway used by prokaryotes, fungi and photosynthetic organisms to convert inorganic sulfate to sulfide, which is further incorporated into carbon skeletons of amino acids to form cysteine or homocysteine. the pathway is highly regulated in a demand-driven manner; however, this regulation is not necessarily identical in various plant species. therefore, our knowledge of the regulation of sulfate assimilation is reviewed here in detail with emphasis on different plant sp ...200616464881
combination effects of herbicides on plants and algae: do species and test systems matter?risk assessment of herbicides towards non-target plants in europe is currently based solely on tests on algae and floating aquatic plants of lemna sp. effects on terrestrial non-target species is not systematically addressed. the purpose of the present study was to compare combination effects of herbicide mixtures across aquatic and terrestrial test systems, and to test whether results obtained in the traditional aquatic test systems can be extrapolated to the terrestrial environment. this was d ...200717304633
exposure to radiofrequency radiation induces oxidative stress in duckweed lemna minor l.widespread use of radiofrequency radiation emitting devices increased the exposure to electromagnetic fields (emfs) from 300 mhz to 300 ghz. various biological effects of exposure to these fields have been documented so far, but very little work has been carried out on plants. the aim of the present work was to investigate the physiological responses of the plant lemna minor after exposure to radiofrequency emfs, and in particular, to clarify the possible role of oxidative stress in the observed ...200717825879
effect of ph and temperature on the uptake of cadmium by lemna minor l. 20071932869
ecotoxicity monitoring of hydrocarbon-contaminated soil during bioremediation: a case study.the ecotoxicity of hydrocarbon-contaminated soil originating from a brownfield site was evaluated during a 17-month biodegradation pilot test. the initial concentration of total petroleum hydrocarbons (tphs) in the soil was 6380 microg/g dry weight. an amount of 200 kg soil was inoculated with 1.5 l of the bacterial preparation gem-100 containing pseudomonas sp. and acinetobacter sp. strains (5.3 x 10(10) cfu.ml(-1)) adapted to diesel fuel. the concentration of tphs in the soil decreased by 65.5 ...200717106791
expression and characterization of acidothermus cellulolyticus e1 endoglucanase in transgenic duckweed lemna minor 8627.endoglucanase e1 from acidothermus cellulolyticus was expressed cytosolically under control of the cauliflower mosaic virus 35s promoter in transgenic duckweed, lemna minor 8627 without any obvious observable phenotypic effects on morphology or rate of growth. the recombinant enzyme co-migrated with the purified catalytic domain fraction of the native e1 protein on western blot analysis, revealing that the cellulose-binding domain was cleaved near or in the linker region. the duckweed-expressed ...200717127051
a long-term copper exposure on freshwater ecosystem using lotic mesocosms: primary producer community responses.copper is commonly used as an algicide and plant herbicide in the aquatic environment. despite of its frequent application in rivers, ponds and lakes, few studies have been performed at the ecosystem level on primary producers' communities. a long-term lotic mesocosms study was carried on, in 20m long channels, under environmentally realistic concentrations of copper (0, 5, 25 and 75 microgl(-1)) delivered continuously for 18 months. results showed significant effects at the 25 and 75 microgl(-1 ...200717239970
toxicity assessment of heavy metal mixtures by lemna minor l.the discharge of untreated electroplating wastewaters directly into the environment is a certain source of heavy metals in surface waters. even though heavy metal discharge is regulated by environmental laws many small-scale electroplating facilities do not apply adequate protective measures. electroplating wastewaters contain large amounts of various heavy metals (the composition depending on the facility) and the ph value often bellow 2. such pollution diminishes the biodiversity of aquatic ec ...200717610935
comparative toxicity of the phytotoxins (8r,16r)-(-)-pyrenophorin and (5s,8r,13s,16r)-(-)-pyrenophorol on aquatic organisms.the acute toxicities of the fungal phytotoxins (8r,16r)-(-)-pyrenophorin and (5s,8r,13s,16r)-(-)-pyrenophorol on vibrio fischeri, oscillatoria perornata, pseudokirchneriella subcapitata, lemna minor and artemia fransiscana were evaluated. (8r,16r)-(-)-pyrenophorin was more toxic than (5s,8r,13s,16r)-(-)-pyrenophorol to v. fischeri, o. perornata, l. minor and a. fransiscana. the highest acute toxicity of (8r,16r)-(-)-pyrenophorin was exhibited on v. fischeri (5 min median effective concentration ...200717898918
performance of duckweed (lemna minor l.) on different types of wastewater treatment.duckweed (lemna minor l.) has a wide application in turkey having suitable climatic conditions. in this study, the growth of duckweed was assessed in laboratory scale experiments. they were fed with municipal and industrial wastewater at constant temprature. cod, total nitrogen (tn), total phosphorus (tp) and ortho-phosphate (op) removal efficiencies of the reactors were monitored by sampling influent and effluent of the system. removal efficiency in this study reflects optimal results: 73-84% c ...200717915771
ecotoxicological evaluation of cork-boiling wastewaters.toxicity tests with vibrio fischeri, daphnia magna, and lemna minor were used to evaluate acute and chronic toxicity of cork-boiling wastewaters and correlation analysis was performed with physicochemical parameters. acute toxicity values (ec(50)) ranged from 2.3% to 24.2% in the microtox test and from 4.4% to 29.5% in the daphnia test. according to these values, 78% of the samples were classified as acutely toxic to the most sensitive species (v. fischeri). significant correlation was obtained ...200716620978
a review of independent action compared to concentration addition as reference models for mixtures of compounds with different molecular target sites.from a theoretical point of view, it has often been argued that the model of independent action (ia) is the most correct reference model to use for predicting the joint effect of mixtures of chemicals with different molecular target sites. the theory of ia, however, relies on a number of assumptions that are rarely fulfilled in practice. it has even been argued that, theoretically, the concentration addition (ca) model could be just as correct. in the present study, we tested the accuracy of bot ...200818271647
an assessment of the potential toxicity of runoff from an urban roadscape during rain events.the potential negative impact of urban storm water on aquatic freshwater ecosystems has been demonstrated in various studies with different types of biological methods. there are a number of factors that influence the amount and bioavailability of contaminants in storm water even if it is derived from an area with a fairly homogenous land use such as a roadscape where a variation in toxicity during rain events might be expected. there are only a few previous investigations on the toxicity of hig ...200818504838
concentrations of heavy metals and aquatic macrophytes of govind ballabh pant sagar an anthropogenic lake affected by coal mining effluent.five heavy metals cu, cd, mn, pb and hg were found in high concentration from three sampling sites located in asia's largest anthropogenic lake govind ballabh pant gbp sagar. concentrations of these heavy metals were measured in water, bottom sediment and in different parts of the aquatic macrophytes collected from the reservoir. plants collected from the lake were eichhornia crassipes, azolla pinnata, lemna minor, spirodela polyrrhiza, potamogeton pectinatus, marsilea quadrifolia, pistia strati ...200817674134
toxicity and metal speciation in acid mine drainage treated by passive bioreactors.sulfate-reducing passive bioreactors treat acid mine drainage (amd) by increasing its ph and alkalinity and by removing metals as metal sulfide precipitates. in addition to discharge limits based on physicochemical parameters, however, treated effluent is required to be nontoxic. acute and sublethal toxicity was assessed for effluent from 3.5-l column bioreactors filled with mixtures of natural organic carbon sources and operated at different hydraulic retention times (hrts) for the treatment of ...200818290688
antibacterial, antifungal, insecticidal, cytotoxicity and phytotoxicity studies on indigofera gerardiana.the antibacterial, antifungal, acute cytotoxicity, phytotoxicity and insecticidal profile of the crude extract and various fractions of indigofera gerardiana have been studied. six bacterial and fungal strains were used, of which samonella typhi and microsporum canis were the most susceptible strains with mics 0.37 mg/ml and 0.09 mg/ml, respectively. the crude extract and the fractions showed low insecticidal activity against sitophilus oryzae, rhyzopertha dominica and callosbruchus analis but n ...200918825532
bioassays for evaluating the water-extractable genotoxic and toxic potential of soils polluted by metal smelters.physicochemical analyses of polluted soils are limited in their ability to determine all hazardous compounds, their bioavailability, and their combined effects on living organisms. bioassays, on the other hand, can evaluate environmental quality more accurately. this study assesses the genotoxic potential of water extracts from soil polluted with metals (pb, cd, and zn) by the former lead smelter in zerjav, slovenia using comet assay with tetrahymena thermophila and human hepatoma cells (hepg2). ...200918973278
ecophysiological tolerance of duckweeds exposed to copper.although essential for plants, copper can be toxic when present in supra-optimal concentrations. metal polluted sites, due to their extreme conditions, can harbour tolerant species and/or ecotypes. in this work we aimed to compare the physiological responses to copper exposure and the uptake capacities of two species of duckweed, lemna minor (lm(ec1)) and spirodela polyrrhiza (sp), from an abandoned uranium mine with an ecotype of l. minor (lm(ec2)) from a non-contaminated pond. from the lowest ...200919027182
phytotoxic effects of cyanobacteria extract on lemna minor and myriophyllum spicatum phyto-tolerance and superoxide dismutase activity.the research on the effects of microcystins on aquatic plants has increased. some aquatic plants have some tolerance to microcystins but the mechanism of the tolerance is still unknown. in this experiment, we used microcystins of different concentrations to study the toxic effect in lemna minor and myriophyllum spicatum. experiments were carried out with a range of microcysitns levels (equivalent to 0, 0.1, 0.5, 1.0, and 4.3 mg/l). the growth of l. minor (as fresh weight) and chlorophyll a conte ...200918623078
bioassays for the evaluation of landfill leachate toxicity.this article reviews the application of bioassays for assessing the toxicity hazard posed by landfill leachate discharged to an aquatic environment. landfill leachate is a complex mixture of chemicals; thus it is difficult to assess the risk posed to aquatic wildlife using standard chemical identification techniques, such as gas chromatography-mass spectroscopy (gc-ms). from this review it is clear that toxicity testing, using species that represent the different trophic levels, is a superior wa ...200919117211
heavy metals in water, sediments and wetland plants in an aquatic ecosystem of tropical industrial region, india.concentrations of heavy metals (cu, cr, fe, pb, zn, hg, ni, and cd) and macronutrients (mn) were measured in industrial effluents, water, bottom sediments, and wetland plants from a reservoir, govind ballabh (g.b.) pant sagar, in singrauli industrial region, india. the discharge point of a thermal power plant, a coal mine, and chlor-alkali effluent into the g.b. pant sagar were selected as sampling sites with one reference site in order to compare the findings. the concentrations of heavy metals ...200918998227
acute toxicity of metals and reference toxicants to a freshwater ostracod, cypris subglobosa sowerby, 1840 and correlation to ec(50) values of other test models.the ostracod cypris subglobosa sowerby, 1840 static bioassay test on the basis of a 48h of 50% of immobilization (ec(50)) has been used to measure the toxicity of 36 metals and metalloids and 12 reference toxicants. among the 36 metals and metalloids, osmium (os) was found to be the most toxic in the test while boron (b), the least toxic. the ec(50) values of this study revealed positive linear relationship with the established test models of cladoceran (daphnia magna), sludge worm (tubifex tubi ...200919683870
biological screening of euphorbia helioscopia l.the present research is preliminary biological screening of euphorbia helioscopia l. (euphorbiaceae). dichloromethane and methanol extracts of the aerial parts of the plant were investigated for their antioxidant, antifungal, antibacterial and phytotoxic activities. dichloromethane extract exhibited significant activity against fusarium solani with 90% inhibition, where as the same extract also showed non-significant activity against salmonella typhi and bacillus subtilis. methanolic extract has ...200919339230
study of use of different types of hydrogen peroxides (2006-2008).hydrogen peroxides are commonly used in greenhouses for cleaning purposes and disinfection of irrigation water systems, i.e., to prevent clogging by duckweed (lemna minor), algae and other (micro)organisms. this use contains a potential risk of involuntary contact to the plants, e.g., to roots through irrigation or to the plant leaves through accidental droplets (spraying mist). to help growers to maximize disinfection with minimal risks, the efficacy and plant safety of a variety of commercial ...200920222582
degradation and ecotoxicity of the biomedical drug artemisinin in soil.the plant artemisia annua l. is cropped in many countries for production of the antimalarial drug artemisinin. artemisinin is phytotoxic and has insecticidal activity. large-scale cultivation of a. annua may cause transfer of artemisinin to soil and, hence, may affect both soil organisms and the aquatic environment if the compound is leachable. in the present study, a new method for extraction of artemisinin from soil was developed, and field concentrations and degradation kinetics of artemisini ...200919391681
sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5'-phosphosulphate reductase under stress and in transgenic poplar plants overexpressing gamma-ecs, so, or apr.sulphate assimilation provides reduced sulphur for the synthesis of cysteine, methionine, and numerous other essential metabolites and secondary compounds. the key step in the pathway is the reduction of activated sulphate, adenosine 5'-phosphosulphate (aps), to sulphite catalysed by aps reductase (apr). in the present study, [(35)s]sulphur flux from external sulphate into glutathione (gsh) and proteins was analysed to check whether apr controls the flux through the sulphate assimilation pathway ...201019923196
heavy metal pollution in lentic ecosystem of sub-tropical industrial region and its phytoremediation.metals and several physicochemical parameters, from four sampling sites in a tropical lake receiving the discharges from a thermal power plant, a coal mine and a chlor-alkali industry, were studied from 2004-2005. pertaining to metal pollution, the site most polluted with heavy metals was belwadah, i.e., waters and sediments had the highest concentration of all the metals examined. the reference site was characterized by the presence of low concentrations of metals in waters and in sediments. fu ...201020734618
phytoremediation of heavy metals in a tropical impoundment of industrial region.aquatic pollution poses a serious challenge to the scientific community worldwide, since lakes or reservoirs find multifarious use and most often their water is used for drinking, bathing, irrigation, and aquaculture. nine metals and several physicochemical parameters, from four sampling sites in a tropical lake receiving the discharges from a thermal power plant, a coal mine, and a chlor-alkali industry, were studied from 2004 to 2005. pertaining to metal pollution, the site most polluted with ...201019430918
comparison of a test battery for assessing the toxicity of a bleached-kraft pulp mill effluent before and after secondary treatment implementation.pulp and paper mill effluents may cause harmful effects to the aquatic environment due to the combined influence of physical factors, toxic compounds, and nutrient enrichment. in the present study, the effectiveness of secondary treatment in reducing the toxicity of an elemental chlorine-free bleached-kraft pulp mill effluent was evaluated. to characterize the toxicity of the effluent, before and after the implementation of secondary treatment, a battery of tests with organisms bearing different ...201019242815
whole effluent assessment of industrial wastewater for determination of bat compliance. part 2: metal surface treatment industry.toxicity testing has become a suitable tool for wastewater evaluation included in several reference documents on best available techniques of the integrated pollution prevention and control (ippc) directive. the ippc directive requires that for direct dischargers as well as for indirect dischargers, the same best available techniques should be applied. within the study, the whole effluent assessment approach of ospar has been applied for determining persistent toxicity of indirectly discharged w ...201020127188
effects of cement flue dust from a cement factory on stress parameters and diversity of aquatic plants.cement kiln dusts, made of a complex mixture of elements, include high levels of heavy metals such as fluoride, magnesium, lead, cadmium, nickel, zinc, copper, beryllium and some toxic compounds. because of the toxic element compositions and radioactive isotope properties of cement kiln dusts, not only terrestrial but also aquatic ecosystems are subjected to greater stress. in the present paper, we investigated the effects of pollution caused by askale-erzurum cement factory (cf) on the stress p ...201020430819
the chronic toxicity of molybdate to freshwater organisms. i. generating reliable effects data.the european union regulation on registration, evaluation, authorization and restriction of chemical substances (reach) (ec, 2006) requires the characterization of the chronic toxicity of many chemicals in the aquatic environment, including molybdate (moo(4)(2-)). our literature review on the ecotoxicity of molybdate revealed that a limited amount of reliable chronic no observed effect concentrations (noecs) for the derivation of a predicted no-effect concentration (pnec) existed. this paper pre ...201020813395
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