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nucleic acid precursor requirements of mycoplasma laidlawii. 196214490761
fatty acid requirements of mycoplasma laidlawii. 196314114935
nutritional requirements and metabolism of mycoplasma laidlawii. 196313973365
glucose metabolism of two strains of mycoplasma laidlawii.castrejon-diez, jaime (tulane university school of medicine, new orleans, la.), thelma n. fisher, and earl fisher, jr. glucose metabolism of two strains of mycoplasma laidlawii. j. bacteriol. 86:627-636. 1963.-two strains of mycoplasma laidlawii were incubated in systems containing d-glucose-c(14); carbon dioxide, acetate, pyruvate, and lactate were isolated from appropriate fluids after resting-cell and growth experiments. in resting-cell experiments, radioactivity recoveries were shown to be 9 ...196314066454
defined medium for mycoplasma laidlawii.tourtellotte, mark e. (university of connecticut, storrs), harold j. morowitz, and phil kasimer. defined medium for mycoplasma laidlawii. j. bacteriol. 88:11-15. 1964.-a defined medium for the pleuropneumonia-like organism mycoplasma laidlawii b is described in which absolute requirements for coenzyme a and longchain fatty acids were demonstrated. this organism did not require cholesterol or macromolecules of high molecular weight, but did show a growth requirement for peptides. optimal growth i ...196414197875
mycoplasma laidlawii var. inocuum comb. nov. 19645874544
carotenoids and cholesterol in membranes of mycoplasma laidlawii. 19655882653
use of dialyzing culture technique for high yield of mycoplasma.pollock, mary e. (university of minnesota, minneapolis). use of dialyzing culture technique for high yield of mycoplasma. j. bacteriol. 90:1682-1685. 1965.-the saprophytic pleuropneumonia-like organism, mycoplasma laidlawii, type a, was cultivated within a dialyzing membrane suspended in a reservoir of uninoculated medium. generation time was not decreased by this method, but the period of active growth was prolonged, and the final yield was 10-fold that attained by the usual cultural methods. a ...19655322724
membrane subunits of mycoplasma laidlawii and their assembly to membranelike structures. 19655216355
morphology of mycoplasma laidlawii type a. ii. effect of glucose on growth and cellular morphology.anderson, d. l. (university of minnesota, minneapolis), m. e. pollock, and l. f. brower. morphology of mycoplasma laidlawii type a. ii. effect of glucose on growth and cellular morphology. j. bacteriol. 90:1768-1777. 1965.-cells of mycoplasma laidlawii a grown in soy peptone-yeast extract (sp-ye) broth reached higher maximal titers than cells grown in sp-ye supplemented with 0.2% glucose, and remained viable longer than glucose-grown cells. moreover, addition of glucose to sp-ye markedly affecte ...19655854595
morphology of mycoplasma laidlawii type a. i. comparison of electron microscopic counts with colony-forming units.anderson, d. l. (university of minnesota, minneapolis), m. e. pollock, and l. f. brower. morphology of mycoplasma laidlawii type a. i. comparison of electron microscopic counts with colony-forming units. j. bacteriol. 90:1764-1767. 1965.-cells of mycoplasma laidlawii a grown in dialyzing flask cultures were counted with the electron microscope by use of a spray technique which deposited mixtures of polystyrene latex of known concentration and m. laidlawii in discrete droplet patterns on specimen ...19655322728
effect of cholesterol on the sensitivity of mycoplasma laidlawii to the polyene antibiotic filipin.weber, morton m. (st. louis university school of medicine, st. louis, mo.), and stephen c. kinsky. effect of cholesterol on the sensitivity of mycoplasma laidlawii to the polyene antibiotic filipin. j. bacteriol. 89:306-312. 1965.-the polyene antibiotic, filipin, inhibited growth and caused lysis of mycoplasma laidlawii cells which had been cultured in the presence of cholesterol. the antibiotic did not inhibit growth and did not promote lysis of the organism when grown in the absence of cholest ...196514255695
glucose-containing phospholipids in mycoplasma laidlawii, strain b. 196514280456
the action of amphotericin b on mycoplasma laidlawii. 196514332454
diphosphatidyl glycerol in mycoplasma laidlawii. 196516562032
growth inhibition of mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol.smith, paul f. (university of south dakota, vermillion), and carl v. henrikson. growth inhibition of mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol. j. bacteriol. 91:1854-1858. 1966.-compounds which inhibit enzymatic reactions in the biosynthetic pathway to carotenoids inhibited growth of a sterol-nonrequiring species, mycoplasma laidlawii, strain b, and m. hominis, strain 07. since m. hominis lacks the enzymes for polyterpene biosynthesis, the inhibitory co ...19665937241
influence of lipid components of mycoplasma laidlawii membranes on osmotic fragility of cells.razin, s. (university of connecticut, storrs), m. e. tourtellotte, r. n. mcelhaney, and j. d. pollack. influence of lipid components of mycoplasma laidlawii membranes on osmotic fragility of cells. j. bacteriol. 91:609-616. 1966.-lipid composition of mycoplasma laidlawii membranes could be significantly changed by variations in the growth medium. the effect of these changes on the osmotic fragility of the cells was studied. cholesterol, incorporated into the membrane from the growth medium, had ...19665883100
adenosine triphosphatase activity of mycoplasma membranes.rottem, shlomo (hebrew university, jerusalem, israel), and shmuel razin. adenosine triphosphatase activity of mycoplasma membranes. j. bacteriol. 92:714-722. 1966.-adenosine triphosphatase activity of mycoplasma laidlawii, m. gallisepticum, and mycoplasma sp. strain 14 was confined to the cell membrane. the enzymatic activity was dependent on magnesium, but was not activated by sodium and potassium. ouabain did not inhibit the adenosine triphosphatase activity of the mycoplasmas, and did not int ...19664224219
control of nadh oxidation by adp in membranes from mycoplasma laidlawii. 19674294270
role of carotenoids and cholesterol in the growth of mycoplasma laidlawii. 19676025421
a comparison of the osmotic and passive permeability properties of mycoplasma laidlawii and mycoplasma hominis. 19676035538
characterization of the plasma membrane of mycoplasma laidlawii. i. sodium dodecyl sulfate solubilization. 19676048810
glucolipids from mycoplasma laidlawii and streptococcus mg. 19676049904
characterization of the plasma membrane of mycoplasma laidlawii. ii. modes of aggregation of solubilized membrane components. 19676058126
the lipid composition of mycoplasma laidlawii strain b.1. total lipid was extracted from mycoplasma laidlawii strain b with chloroform-methanol mixtures and fractionated into neutral lipid, glycolipid and phospholipid components by chromatography on silicic acid. 2. saponification of the glycolipid fraction, which represented nearly half of the total lipid, yielded two glycosides for which the structures o-alpha-d-glucopyranosyl-(1-->1)-d-glycerol and o-alpha-d-glucopyranosyl-(1-->2)-o-alpha-d-glucopyranosyl-(1-->1)-d-glycerol were established. 3. t ...19684297044
carotenoids as protectors against photodynamic inactivation of the adenosine triphosphatase of mycoplasma laidlawii membranes. 19684234699
characterization of the plasma membrane of mycoplasma laidlawii. iv. structure and composition of membrane and aggregated components. 19685650391
characterization of the plasma membrane of mycoplasma laidlawii. 3. the formation and aggregation of small lipoprotein structures derived from sodium dodecyl sulfate-solubilized membrane components. 19685650390
deoxyribonucleate binding and transformation in mycoplasma laidlawii. 19684865478
macromolecular synthesis and thymineless death in mycoplasma laidlawii b.the relationships between macromolecular synthesis and viability have been studied in the pleuropneumonia-like organism mycoplasma laidlawii b adapted to a semidefined grwoth medium. this organism exhibited an absolute growth requirement for the nucleosides uridine and thymidine, a partial requirement for guanosine and deoxyguanosine, but no requirement for adenosine, deoxyadenosine, cytosine, and deoxycytosine. cytosine and deoxycytosine partially satisfied the requirement for uridine. loss in ...19684881702
an ultrastructural study of mycoplasma laidlawii type a and two strains of mycoplasma laidlawii isolated from the oviducts of dairy cows. 19684888099
physiological and serological comparisons among strains of mycoplasma granularum and mycoplasma laidlawii.mycoplasma granularum strains grew on a medium devoid of animal serum or of serum fractions containing sterols; all strains possessed properties, including carotenoid biosynthesis, similar to those described for m. laidlawii. some common antigenic components were noted among m. granularum and m. laidlawii strains by indirect fluorescent-antibody tests. the growth of m. granularum strains was slightly inhibited by antiserum to m. laidlawii pg-8, and the electrophoretic patterns of cell proteins o ...19684870272
immunological analysis of mycoplasma membranes.the antigens responsible for the production of antibodies to mycoplasma laidlawii and m. gallisepticum causing growth and metabolic inhibition of these organisms were localized in the cell membrane. various membrane fractions were tested for serological activity. membrane lipids were completely or almost completely inactive, whereas several preparations of defatted membrane proteins retained some serological activity, shown by their ability to stimulate metabolic inhibition antibody in rabbits a ...19694981055
characterization of the plasma membrane of mycoplasma laidlawii. v. effects of selective removal of protein and lipid. 19695792241
biosynthesis of glucosyl diglycerides by mycoplasma laidlawii strain b.monoglucosyl diglyceride is synthesized from 1,2-diglyceride and uridine-5'-diphosphoglucose (udp); diglucosyl diglyceride from monoglucosyl diglyceride, and uridine-5'-diphosphoglucose by membranes of mycoplasma laidlawii strain b. all of these enzymatic activities reside in the membrane. membranes solubilized by detergent action or succinylation and acetone powders of membranes were inactive. requirements for mg(2+), udp, and appropriate lipid acceptor were demonstrated for biosynthesis of bot ...19695808075
characterization of the plasma membrane of mycoplasma laidlawii. vi. potassium transport. 19695792242
mycoplasma membrane lipids: variations in fatty acid composition.the fatty acid composition of the membrane polar lipids of mycoplasma laidlawii b can be dramatically altered. these variations reslut in characteristic morphological chlanges, and in most cases the cells remain viable. this organism should provide a useful system for clarifying the role of fatty acyl chains in biological membranes.19695777213
the role of lipids in membrane transport in mycoplasma laidlawii. 19695823712
observations on membranes of mycoplasma laidlawii strain b.the cytoplasmic membrane of mycoplasma laidlawii strain b is solubilized by anionic and nonionic detergents, succinylation, phospholipase a, alkaline phosphatase, trypsin, and chymotrypsin. cationic detergents are without effect, as are chelating agents, even in the presence of high concentrations of monovalent cation. the detergent-solubilized membrane exhibits one peak in the analytical ultracentrifuge, but the sedimentation coefficient is dependent upon concentration of detergent. simple dial ...19695361209
proposal for classifying organisms related to mycoplasma laidlawii in a family sapromycetaceae, genus sapromyces, within the mycoplasmatales. 19695391433
deoxyribonucleic acid homology and relative genome size in mycoplasma.the deoxyribonucleic acid homologies of mycoplasma laidlawii type a and type b, m. pulmonis (#47 and #63), and m. hominis were determined by membrane methodology. the homology data revealed a difference in genome size between m. laidlawii type a and type b. this difference also held with stringent conditions of annealing (high temperature). little or negligible homology was shown to exist between the m. laidlawii strains type a and type b and m. pulmonis strains 47 and 63 and m. hominis. m. homi ...19695370271
calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.both membranes of mycoplasma laidlawii and water dispersions of protein-free membrane lipids exhibit thermal phase transitions that can be detected by differential scanning calorimetry. the transition temperatures are lowered by increased unsaturation in the fatty acid residues, but in each case they are the same for membranes and lipids. the transitions resemble those observed for synthetic lipids in the lamellar phase in water, which arise from melting of the hydrocarbon chains within the phos ...19695257955
repair replication of dna in ultraviolet irradiated mycoplasma laidlawii b. 19695358643
the effect of long chain fatty acid isomers on growth, fatty acid composition and osmotic fragility of mycoplasma laidlawii a. 19695374018
new sero-types of mycoplasma laidlawii isolated from mastitic milk and urogenital tracts of cattle. ii. typing of m. laidlawii by biological and serological methods. 19695392349
d- and l-alanylphosphatidylglycerols from mycoplasma laidlawii, strain b. 19694391533
dna replication in mycoplasma laidlawii b. 19694890600
characteristics of a new sterol-nonrequiring mycoplasma.two mycoplasma strains recovered from tissue culture environments were found to grow in complex media devoid of serum or serum fractions containing cholesterol and in a cholesterol-free synthetic medium. neither strain was capable of synthesizing pigmented carotenoids, although these compounds are present in, and characteristic of, other sterol-nonrequiring mycoplasmas. serological tests and an analysis of their cell protein patterns obtained by gel electrophoresis indicated that the isolates we ...19694892385
rapid determination by light scattering of growth parameters of mycoplasma laidlawii in liquid media.an impediment to progress in the study of the course of growth, the effects of medium components, antibiotics, etc., of mycoplasma has been the cumbersome methods of growth measurement currently in use. heretofore, it required the plating of numerous samples during growth, at least in triplicate, after appropriate dilution, followed by a delay of 2 to 3 days before the colonies developed so that counts could be made. we applied the technique of light scattering to measure the growth of mycoplasm ...19694896103
synthesis and turnover of membrane protein and lipid in mycoplasma laidlawii. 19694307353
mycoplasma laidlawii in human burns.mycoplasma laidlawii was recovered from burns from 13 of 52 patients. in most cases, cocci or bacilli were isolated simultaneously.19695784230
new sero-types of mycoplasma laidlawii isolated from mastitic milk and urogenital tracts of cattle. i. isolation of m. laidlawii. 19695817669
ph-dependent changes in density of plasma membranes of growing mycoplasma laidlawii cells. 197011945407
membrane structure: spin labeling and freeze etching of mycoplasma laidawii.a spin-labeled fatty acid was incorporated in vivo into the polar lipids of mycoplasma laidlawii membranes. the electron paramagnetic resonance signal from either intact cells or their extracted lipids reflected the fatty acid composition of the mycoplasma membranes. comparison of signals from intact cells, gramicidin-treated cells, heat-treated cells, and extracted lipids indicates that a major portion of the membrane lipids is in a semiviscous hydrocarbon environment. the results also show tha ...19704316683
immunoelectrophoretic demonstration of proteolytic activity against human proteins by mycoplasma laidlawii. 19704248067
ultrastructure of mycoplasma laidlawii during culture development.mycoplasma laidlawii b has been studied by phase-contrast and electron microscopy (thin-section and negative staining). exponential-phase cells are filaments with the following structures: surface unit membrane, nuclear material, ribosomes, and intracellular granular region. these cells appear to reproduce by binary fission. in stationary phase, the filamentous cells have numerous constrictions, giving them a beaded appearance. nonviable death-phase cultures contain cellular debris: swollen cell ...19704192565
cholesterol inhibition of isopentenyl pyrophosphate delta3, delta2-isomerase in mycoplasma laidlawii.cholesterol inhibits isopentenyl delta(3),delta(2)-isomerase of mycoplasma laidlawii in an apparently competitive fashion. the conversion of mevalonic acid to isopentenyl pyrophosphate is slightly stimulated. organisms grown in the presence of mevalonic-2-(14)c acid contain small amounts of radio-label in nucleic acid and protein fractions. most of the label is found in the lipids and is reduced dramatically in organisms grown with cholesterol. no significant accumulation of phosphorylated inter ...19704316364
[isolation of a mycoplasma strain similar to mycoplasma laidlawii from primary calf kidney cell culturs]. 19704101148
the synthesis of long-chain fatty acids by a cell-free system from mycoplasma laidlawii a. 19704391501
metabolic turnover of the polar lipids of mycoplasma laidlawii strain b.the fatty acyl groups of the membrane polar lipids of mycoplasma laidlawii b were radioactively labeled by growth in the presence of (14)c-palmitic, oleic, or linoleic acids. no loss of radioactivity from any of the polar lipids occurred during incubation of radiolabeled cells in growth medium containing various nonradioactive fatty acids, although growth and lipid biosynthesis continued throughout the incubation period. the metabolism of the glucose and phosphate moieties was also studied in a ...19705411758
isolation of a virus infecting a strain of mycoplasma laidlawii. 19705418254
characterization of the plasma membrane of mycoplasma laidlawii. vii. phase transitions of membrane lipids. 19705473498
native and reformed mycoplasma laidlawii membranes compared by freeze-etching. 19705473500
the effect of alterations in fatty acid composition and cholesterol content on the permeability of mycoplasma laidlawii b cells and derived liposomes. 19705473512
incorporation and elongation of fatty acid isomers by mycoplasma laidlawii a. 19705412665
the relationship between fatty acid structure and the positional distribution of esterified fatty acids in phosphatidyl glycerol from mycoplasma laidlawii b. 19705417177
x-ray diffraction studies of phase transitions in the membrane of mycoplasma laidlawii. 19705413340
amended nomenclature for strains related to mycoplasma laidlawii. 19705487182
acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the mycoplasmatales.mycoplasmas recovered from tissue cultures and previously shown to belong to the sterol-nonrequiring group of mycoplasmas have been further characterized. the biological and serological properties of these strains show them to be clearly distinct from acholeplasma laidlawii and a. granularum, two species of sterol-nonrequiring mycoplasmas recently reclassified. it is proposed that the newly described mycoplasmas be designated acholeplasma axanthum, sp. n.19704919991
immunogenicity of mycoplasma pneumoniae glycolipids: a novel approach to the production of antisera to membrane lipids.the glycolipid haptens of mycoplasma pneumoniae were bound to membrane proteins of acholeplasma laidlawii (formerly mycoplasma laidlawii) by reaggregation. this process consisted of the solubilization of lipid-depleted a. laidlawii membranes and m. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate, and dialysis of the mixed solutions against 20 mm mg(2+). the hybrid reaggregate, collected by centrifugation, was highly immunogenic in rabbits, eliciting the production of a high titer of antib ...19704943173
cholesterol requirement of mycoplasmas.cholesterol requirement for growth of mycoplasmas was tested in a serum-free medium supplemented with albumin, l-arginine, palmitic acid, and various concentrations of cholesterol dissolved in tween 80. in cases in which tween 80 was shown to inhibit growth, the test medium was supplemented with cholesterol dissolved in ethanol. of the 31 species examined, all but mycoplasma laidlawii, m. granularum, and mycoplasma species strain s-743 exhibited a growth response to cholesterol. no requirement f ...19704911537
calorimetric detection of a membrane-lipid phase transition in living cells.the membrane lipids in living mycoplasma laidlawii exhibit a phase transition characteristic of that from crystal to liquid crystal within the bilayer conformation. the transition occurs at the same temperature in viable organisms, membranes isolated from the organisms, and isolated membrane lipids. the enthalpy of the transition in the membrane is compared with that of an aqueous suspension of isolated membrane lipids. the result is consistent with presence of an extended lipid bilayer in the n ...19705420540
[biochemical investigations on 10 strains of mycoplasma laidlawii]. 19715105631
peptidase activity in the membranes of mycoplasma laidlawii. 19715117556
lipid bilayer structure in the membrane of mycoplasma laidlawii. 19715088924
[etiology and diagnosis of porcine mycoplasmosis. 3. pathological, anatomical and histological studies of experimentally infected suckling pigs]. 19715131929
characterization of the mycoplasma membrane proteins. i. reaggregation of solubilized membrane proteins of acholeplasma laidlawii. 19715141123
transfer ribonucleic acid from mycoplasma laidlawii a. 19715545013
[serologic differentiation between acholeplasma laidlawii (mycoplasma laidlawii) and acholeplasma granularum (mycoplasma granularum) strains using agglutination and precipitation]. 19715169114
[fractionation of the population of mycoplasma laidlawii in urografin density gradient]. 19715567637
kinetics of sodium dodecyl sulfate solubilization of mycoplasma laidlawii plasma membranes.the kinetics of sodium dodecyl sulfate solubilization of aqueous suspensions of mycoplasma laidlawii membranes have been investigated by light scattering in a stopped-flow apparatus. there was evidence of direct interaction between the membranes and sodium dodecyl sulfate micelles above the critical micelle concentration, although of lower order kinetically than with monomeric dodecyl sulfate anions below the critical micelle concentration. the activation energy remained the same in either case, ...19715289357
the microscopic examination of the apollo-11 and -12 sample and the quest for life forms. 19715142391
[the effects of glucose on mycoplasma]. 19714947989
[ultrastructure and development of colonies of a strain of mycoplasma laidlawii in solid media]. 19715002964
[effect of mycoplasma-viral infection of a primary culture of chick embryo cells on interferon production induced by langat virus]. 19715167284
presence of two dna populations in mycoplasma laidlawii. 19715551322
electron microscopy of solubilized acholeplasma laidlawii membrane proteins reaggregated with mycoplasma pneumoniae glycolipids. 19715579139
induction of alpha-glucosidase in mycoplasma laidlawii a. 19715283618
structural comparisons of native and reaggregated membranes from mycoplasma laidlawii and erythrocytes by x-ray diffraction and nuclear magnetic resonance techniques. 19715159791
structural comparisons of native and reaggregated membranes from mycoplasma laidawii and erythrocytes using a fluorescence probe. 19715159792
immunogenicity of cytolipin h aggregated with acholeplasma laidlawii membrane proteins. 19715165763
incorporation of penicillinase into reaggregated mycoplasma membranes. 19715003616
isolation and identification of porcine mycoplasma in switzerland. 19714946169
mycoplasmas in cell cultures from rheumatoid synovial membranes.ten strains of myocoplasmas were recovered from cultures of synovium or cultures inoculated with synovial fragments from rheumatoid arthritis and one from osteo-arthritis. the source of the organisms is not known. patients with rheumatoid arthritis had no complement-fixing antibody and no fluorescent staining antibody against the mycoplasmas isolated and no mycoplasma antigen was detected by immunofluorescence in sections of synovia and in synovial fluids.the strains isolated were of two main se ...19714948122
a membrane-bound lysophospholipase from mycoplasma laidlawii strain b. 19714993729
mycoplasmatales virus-laidlawii 2, a new virus isolated from acholeplasma laidlawii. 19714106810
phosphatase activity of some mycoplasma species. 19714361836
characterization of the mycoplasma membrane proteins. ii. solubilization and enzymic activities of acholeplasma laidlawii membrane proteins. 19714334752
phase transitions and bilayer structure of mycoplasma laidlawii b. 197111945568
production and properties of antisera to membrane glycolipids of mycoplasma pneumoniae.the glycolipid haptens of mycoplasma pneumoniae became immunogenic when bound to membrane proteins of acholeplasma laidlawii by reaggregation. this process consisted of the solubilization of lipid-depleted a. laidlawii membranes and m. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed solutions against 20 mm mg(2+). the antibodies produced in rabbits to the reaggregated glycolipids inhibited the metabolism of m. pneumoniae, fixed complement with m. pneumoniae glyco ...197116557990
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