Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| odontomyces viscosus, gen. nov., spec. nov., a filamentous microorganism isolated from periodontal plaque in hamsters. | 1965 | 5896131 | |
| toxic properties of the cell wall of gram-positive bacteria. | the biological activity of odontomyces viscosus, which has been reported to cause periodontal disease in hamsters, was examined. the microorganism was cultured anaerobically in brain heart infusion broth, and the cells were harvested. the washed cells were injected intradermally into the abdomen of rabbits. after 72 hr, a well-defined, firm, raised nodule (about 1.0 by 1.5 cm) with an erythematous border was seen at the injection site. suspensions of cell wall and cytoplasmic material were injec ... | 1967 | 5335900 |
| production of an extracellular levan by odontomyces viscosus. | 1967 | 5227886 | |
| plaque formation and implantation of odontomyces viscosus in hamsters fed different carbohydrates. | 1969 | 5259424 | |
| identification of human strains of actinomyces viscosus. | catalase-positive actinomycetes which closely resemble the "hamster organism" described by howell have been isolated from dental calculus and other human sources. these cultures could not be distinguished from the hamster strains on the basis of morphology, oxygen requirements, biochemical reactions, or cell wall composition. these human isolates have been classified with the hamster strains as actinomyces viscosus. the strains from hamster and human sources fell into two serotypes. serotype 1 c ... | 1969 | 4896106 |
| levan formation by odontomyces viscosus. | 1969 | 5261310 | |
| [odontomyces viscosus, gen. nov. spec. nov]. | 1970 | 5274251 | |
| a cariogenic actinomyces viscosus--a bacteriological and gnotobiotic study. | 1971 | 5117164 | |
| chemical and antigenic properties of the cell wall of actinomyces viscosus (strain t6). | 1972 | 4506556 | |
| [dental caries in the gnotobiotic rat inoculated with actinomyces viscosus and actinomyces naeslundii]. | 1972 | 4506978 | |
| actinomyces viscosus as an agent of actinomycosis in the dog. | 1972 | 4557020 | |
| actinomyces viscosus in man. | 1972 | 4110339 | |
| method for preclinical evaluation of antiplaque agents. | a preclinical method for the evaluation of antibacterial agents for use against dental plaques associated with caries and periodontal disease is proposed. the method is applicable to screening agents and to defining, in vitro, the minimal conditions required for maximal antiplaque effect. as a model of antiplaque agents, chlorhexidine was assessed in vitro against preformed plaques of microorganisms conducive to dental caries and periodontal disease. the agent was bactericidal to plaques of nine ... | 1972 | 4670477 |
| serological identification of rodent strains of actinomyces viscosus and their relationship to actinomyces of human origin. | 1972 | 4114782 | |
| identification of actinomyces, arachnia, bacterionema, rothia, and propionibacterium species by defined immunofluorescence. | fractionated fluorescein-isothiocyanate (fitc)-conjugated immunoglobulin g (dye-to-protein ratio <10), produced against whole cells of actinomyces spp., arachnia, bacterionena, rothia, and propionibacterium spp., give species-specific conjugates with controlled nonspecific staining reactions when appropriately diluted on the basis of their antibody content (10 mg/ml). using this standardization in immunofluorescence, serotype-specific conjugates are also available after dilution for all serotype ... | 1973 | 4123707 |
| actinomyces viscosus in a branchial cyst. | 1973 | 4751713 | |
| numerical taxonomy and laboratory identification of bacterionema matruchotii, rothia dentocariosa, actinomyces naeslundii, actinomyces viscosus, and some related bacteria. | 1973 | 4579125 | |
| plaque formation and dental caries in gnotobiotic and spf osborne-mendel rats associated with actinomyces viscosus. | 1974 | 4525551 | |
| canine actinomycosis due to actinomyces viscosus: report of six cases. | 1974 | 4496897 | |
| degradation of sucrose by whole cells and plaque of actinomyces naeslundii. | whole cells of actinomyces naeslundii atcc 12104, either in a dispersed form or in the form of plaque, enzymatically degraded sucrose to glucose and fructose. washed whole cells expressed beta-fructofuranosidase specificity and hydrolyzed sucrose to essentially equimolar quantities of glucose and fructose. the cells readily hydrolyzed sucrose, raffinose, and actinomyces viscosus or aerobacter levanicum levan, but did not degrade melezitose, maltose, alpha-methyl-d-glucoside, melibiose, glucose-1 ... | 1974 | 4611924 |
| chemical structure of the cell wall peptidoglycan of actinomyces viscosus. | 1974 | 4531862 | |
| reactions in the periodontium to continuous antigenic stimulation in sensitized gnotobiotic rats. | the purpose of our experiment was to evaluate the destructive potential of a strain of actinomyces viscosus on the periodontium of sensitized rodents, describing the induced lesions on the basis of quantitative cytology. the experimental design comprised in principle the following procedures. young germfree rats were immunized either by intravenous or intradermal injections with heat-killed cells of a. viscosus ny 1 or sham immunized intradermally with physiological saline. after a period suitab ... | 1974 | 4426700 |
| extracellular polysaccharides of actinomyces viscosus. | polysaccharide(s) have been isolated from supernatants of broth cultures of actinomyces viscosus, strain t6. the organism produces significant quantities (2 mg/mg of cell [dry weight], 108 mg/liter) of this polysaccharide in the absence of sucrose. chemical analysis indicated that n-acetylglucosamine (62%) was the major component; galactose (7%), glucose (4%), uronic acid (3%), and smaller amounts of glycerol, rhamnose, arabinose, and xylose were also present. the polysaccharide(s) is only sligh ... | 1974 | 4851986 |
| a slime-producing microorganism in dental plaque of rats, selected by glucose feeding. chemical composition of extracellular slime elaborated by actinomyces viscosus, strain nyl. | 1974 | 4276010 | |
| ultrastructure of actinomyces viscosus and actinomyces naeslundii. | 1974 | 4132890 | |
| galactosyl-diglyceride from actinomyces viscosus. | 1974 | 4366087 | |
| regulation of lactate dehydrogenase activity in actinomyces viscosus by adenosine-5'-triphosphate. | 1974 | 4374177 | |
| mutanolysin, bacteriolytic agent for cariogenic streptococci: partial purification and properties. | mutanolysin partially purified from the culture filtrate of streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near ph 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. mutanolysin exhibited maximal lytic activity at 60 c in the ph range 6.5 to 7.0 and was stable at 50 c in the acid range. n-bromosuccinimide caused complete inhibition of lytic activity at 1 mm, whereas calcium and magnesium ions at the same concen ... | 1974 | 15828186 |
| two-way cross-reactions between serotypes of actinomyces viscosus. | 1975 | 47742 | |
| purification, characterization, and regulation of a nicotinamide adenine dinucleotide-dependent lactate dehydrogenase from actinomyces viscosus. | a nicotinamide adenine dinucleotide-specific l-(+)-lactate dehydrogenase (ldh) (ec 1.11.27) from actinomyces viscosus t-6-1600 was purified approximately 110-fold by a combination of diethylaminoethyl-cellulose and 0.5 m agarose a column chromatography. the ldh was stable at 26 c, but was quite labile at temperatures below 5 c. the enzyme had a molecular weight of 100,000 +/- 10,000 as determined by 0.5 m agarose molecular exclusion chromatography and showed optimum activity between ph 5.5 and 6 ... | 1975 | 238940 |
| effect of actinomyces viscosus on the establishment and symbiosis of streptococcus mutans and streptococcus sanguis in spf rats on different sucrose diets. | 1975 | 123171 | |
| levan formation by whole cells of actinomyces viscosus atcc 15987. | 1975 | 1057581 | |
| identification of actinomyces viscosus from canine infections. | actinomyces viscosus is a gram-positive, non-acid-fact, facultative, catalase-positive, filamentous, or diphtheroidal microorganism. it was isolated from six canine infections during a period of 1.5 years. the organism was cultured from exudate and flaky granules aspirated from infectious granulomas and empyemas. all cultures grew well aerobically and anaerobically with the addition of 10% carbon dioxide. they fermented lactose, produced catalase and acetylmethylcarbinol, reduced nitrates, hydro ... | 1975 | 1236870 |
| dextran-induced aggregation of actinomyces viscosus. | 1975 | 1066098 | |
| predominant cultivable flora isolated from human root surface caries plaque. | plaque samples were obtained from tooth surfaces exhibiting typical lesions of root surface caries and were immediately cultured by a continuous anaerobic procedure. the bacterial composition of root caries flora was determined on individual samples. representative isolates from each specimen were characterized by morphological and physiological criteria. in addition, fluorescent antibody reagents were used to confirm the identification of streptococcus mutans and actinomyces viscosus. the plaqu ... | 1975 | 1091550 |
| [influence of various anions and biological macromolecules on bacterial aggregation in vitro]. | the effects of various anions on experimental bacterial aggregation has been studied with streptococcus mutans omz 175 and actinomyces viscosus x1, using an experimental system, based on the measurement of optical density in an aqueous polyethyleneglycol medium. monovolent anions (acétate, chloride and fluoride) and sulphate had no effects on bacterial aggregation in salivary concentrations. however carbonate, mono and dihydrogenophosphates gave an important aggregation in relatively small salin ... | 1975 | 1060618 |
| extracellular invertase activity from actinomyces viscosus. | 1975 | 1053762 | |
| initial products in utilization of sucrose by actinomyces viscosus. | 1975 | 1218076 | |
| differential medium for detecting dental plaque bacteria resembling actinomyces viscosus and actinomyces naeslundii. | a medium for detecting colonies of actinomyces viscosus and actinomyces naeslundii in dental plaque samples was developed. the medium (cnac-20) contains 20.0 mug of 3cdso4-8h2o per ml of columbia cna agar base. laboratory strains of a. viscosus grew on cnac-20 in characteristic round, white, smooth, opaque colonies. increasing the cadmium concentration impaired the growth of some a. viscosus strains. stock strains of a. naeslundii and a. israelii grew in colonies of similar white, opaque morphol ... | 1975 | 1184734 |
| importance of actinomyces and certain gram-negative anaerobic organisms in the transformation of lymphocytes from patients with periodontal disease. | dental plaque deposits are known to be potent stimulants of lymphocyte transformation in patients with periodontal disease but not in normal subjects. since plaque deposits consist mainly of whole bacteria, the cell walls of the most commonly found organisms in plaque were tested for their capacity to induce lymphocyte transformation. there was a direct correlation between the severity of peridontal disease and the amount of transformation induced by the cell walls of oral bacteria and by solubi ... | 1976 | 1270144 |
| dextran-mediated interbacterial aggregation between dextran-synthesizing streptococci and actinomyces viscosus. | streptococcus sanguis and streptococcus mutans bind to the surface of actinomyces viscosus, producing large microbial aggregates. aggregates form rapidly and are not easily dissociated by vigorous mixing. the binding is mediated by dextran. glucose-grown streptococci will not aggregate unless they are first mixed with high-molecular-weight dextran. aggregation is induced with dextrans isolated from leuconostoc, s. sanguis, or s. mutans. sucrose-grown streptococci will adhere to a. viscosus witho ... | 1976 | 1279004 |
| antigens and surface components associated with virulence of actinomyces viscosus. | we have isolated a specific cell wall antigen of high molecular weight which appears to be unique to virulent strains of a viscosus and a naeslundii. the antigen is composed of two parts: a polysaccharide moiety containing 6-dot as the major sugar and determinant of serologic specificity, and a small peptide bearing some resemblance to the peptidoglycan. other data indicate a positive correlation between the presence of this antigen and an extrachromosomal piece of dna having most of the propert ... | 1976 | 1060640 |
| establishment and symbiosis of actinomyces viscosus, streptococcus sanguis and streptococcus mutans in germ-free osborne-mendel rats. | 1976 | 1061641 | |
| cell-mediated immune response to products of actinomyces viscosus cultures. | hartley strain guinea pigs were sensitized with 0.5 ml of concentrated cell-free actinomyces viscosus culture supernatant fluids mixed with freund complete adjuvant. fourteen to 16 days later the animals were challenged by intradermal injection with 0.1 ml of the culture supernatant, and the reactions were observed at 4, 8, 16, 24, and 48 h. peritoneal exudate cells from sensitized animals were used for determination of migration inhibition factor, and guinea pig peripheral blood served as a sou ... | 1976 | 971952 |
| endocarditis caused by actinomyces viscosus. | a case of severe endocarditis caused by actinomyces viscosus is described in a previously healthy 70-year-old man. the isolated strain has been characterized by biochemical tests, gas-liquid chromatography and serology. the pathogenic significance of the organism is discussed and the difficulty in reaching a correct diagnosis due to the similarity to species of corynebacterium is pointed out. | 1976 | 1006175 |
| in vivo and in vitro studies on possible pathogenic mechanisms of actinomyces viscosus. | actinomycotic infections are characterized by long-term inflammatory lesions containing large numbers of polymorphonuclear leukocytes (pmns) and mononuclear cells. the pathogenic mechanisms involved in these lesions are not understood. homogenates of actinomyces viscosus (avis) induce an acute inflammatory response with a predominance of pmns within 6 h after injection into the footpads of nonimmunized mice. these homogenates, when tested in vitro, contain potent chemotactic activity for human p ... | 1976 | 971963 |
| a levansucrase from actinomyces viscosus. | 1976 | 1058740 | |
| establishment and distribution of actinomyces viscosus and actinomyces naeslundii in the human oral cavity. | the intraoral establishment and proportional distribution of suspected periodontal pathogens actinomyces viscosus and actinomyces naeslundii were studied using a recently developed differential plating medium, cnac-20. saliva and dental plaque samples were collected from 108 subjects ranging in age from infants to young adults; tongue and buccal mucosa samples were collected from only the adult subjects. catalase-negative a. naeslundii was isolated from 40% of the predentate infants' and almost ... | 1976 | 977124 |
| blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates. | soluble sonicate supernatant preparations were made from actinomyces viscosus (atcc 19246), a. naeslundii (atcc 12104), two strains of veillonella alcalescens (strain hv-1 and a human oral isolate), streptococcus sanguis (atcc 10556), s. mutans (strain 6715-t2), bacteroides melaninogenicus (strain k110), and leptotrichia buccalis (isolated from human dental plaque). these supernatants were characterized with reference to their chemical and antigenic components and their biological activity deter ... | 1976 | 977126 |
| interaction of inflammatory cells and oral microorganisms. iii. modulation of rabbit polymorphonuclear leukocyte hydrolase release response to actinomyces viscosus and streptococcus mutans by immunoglobulins and complement. | in the absence of antiserum, rabbit polymorphonuclear leukocytes (pmns) released lysosomal enzymes in response to actinomyces viscosus (19246) but not to streptococcus mutans (6715). antibodies had a marked modulating influence on these reactions. pmn hydrolase release was significantly enhanced to both organisms when specific rabbit antiserum and isolated immunoglobulin g (igg) were included in the incubations. immune complex f(ab')2 fragments of igg directed against s. mutans agglutinated bac ... | 1976 | 1002300 |
| initial colonization of teeth in monkeys as related to diet. | the initial phases of plaque development on nonretentive tooth surfaces were studied bacteriologically in macaca irus monkeys fed by stomach tube and provided with various oral supplements. except for the oral implantation of streptococcus mutans in some of the animals, the oral flora was not changed prior to the studies. dental plaque was allowed to develop on initially cleaned tooth surfaces for 3 to 5 h. plaque samples were collected and cultured on a number of selective and nonselective agar ... | 1976 | 825462 |
| regulation of lactate dehydrogenase activity in rothia dentocariosa by fructose 1,6-diphosphate and adenosine 5'-triphosphate. | the l-(+)-lactate dehydrogenase from rothia dentocariosa strain 17931 is activated by fructose 1,6-diphosphate and inhibited by adenosine 5'-triphosphate. the enzyme has a molecular weight of 120,000. in these respects, it resembles the lactate dehydrogenase of actinomyces viscosus. | 1976 | 181365 |
| activation of complement by the alternative pathway as a factor in the pathogenesis of periodontal disease. | dental plaque and a bacterium, actinomyces viscosus, isolated from plaque that can reproduce periodontal disease in germ-free rats, are activators of complement by the alternative pathway. it is suggested that this process is involved in the pathogenesis of chronic inflammatory periodontal disease. | 1976 | 62218 |
| blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects. | cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of actinomyces viscosus (av), actinomyces naeslundii (an), veillonella alcalescens (va), leptotrichia buccalis (lb), b ... | 1976 | 10259 |
| levan and levansucrase of actinomyces viscosus. | a levansucrase was demonstrated in the growth medium and in association with the cell surface of actinomyces viscosus. the amount of enzyme produced relative to cell density is not significantly affected by the growth conditions. sugar alcohols inhibit growth of the cells. the levansucrase hydrolyzes sucrose to produce free glucose and levan; some free fructose is also formed. there is no requirement for cofactors. the km for sucrose is 12 mm. a variety of heavy metal ions and two disaccharides, ... | 1977 | 14893 |
| identification, separation, and preliminary characterization of invertase and beta-galactosidase in actinomyces viscosus. | the initial step of disaccharide dissimilation by actinomyces viscosus serotype 2 strain m-100 was studied. sucrase activity was found in the 3,000 x g particulate fraction and the 37,000 x g soluble fraction of the cells, whereas lactase activity was found almost exclusively in the 37,000 x g soluble fraction. neither sucrase nor lactase activity was appreciable in the culture liquor. sucrose phosphorylase, alpha-glucosidase, and polysaccharide synthesis activities were not observed in the solu ... | 1977 | 17574 |
| binding of lectins to streptococcus mutans cells and type-specific polysaccharides, and effect on adherence. | the lectin concanavalin a (con a) agglutinated the cells of 13 of 15 strains of the seven serotypes of streptococcus mutans in an 18-h incubation period. strains of types a, d, f, and g agglutinated within 2 h. strains of a, d, and f were also agglutinated in 2 h by the castor bean lectin rca. s. sanguis, s. salivarius, s. bovis, actinomyces viscosus, a. naeslundii, and lactobacillus plantarum were agglutinated within 2 h. the s. mutans type f polysaccharide was precipitated by con a. the a, b, ... | 1977 | 22491 |
| mitogenic activity of actinomyces viscosus. i. effects on murine b and t lymphocytes, and partial characterization. | actinomyces viscosus homogenate (avis) contins substance(s) which cause spleen cells from conventional and germfree mice to undergo increased dna synthesis. this mitogenic effect is primarily on b cells since spleen cells from nude mice or t-depleted spleen cells from conventional mice respond as strongly as conventional (t + b) spleen cells. mouse thymocytes do not respond mitogenically to avis. it is unlikely that the mitogenic acitivity is due to the presence of lps, since a. viscosus is gram ... | 1977 | 66295 |
| [the mechanism of glucan-induced agglutination of actinomyces viscosus (author's transl)]. | 1977 | 288805 | |
| comparison of antiplaque agents using an in vitro assay reflecting oral conditions. | an in vitro assay is described using saliva-treated bovine enamel slabs for determining the potential of chemotherapeutic agents to adsorb to tooth surfaces and act against plaque-forming bacteria. chlorhexidine was found to inhibit the formation of in vitro plaque by actinomyces viscosus, a naeslundii, streptococcus mutans and s sanguis. actinobolin was found to have marked antibacterial properties but limited adsorptive qualities. | 1977 | 268336 |
| a microbiological study of normal flora of macropod dental plaque. | the percentage bacterial composition of dental plaques from 12 macropods was determined. the major group of organisms was gram-positive filamentous and pleomorphic rods whose median representation was 92.2%. these were mainly actinomyces viscosus (median value 62.5%) and bacterionema matruchotii (median value 23.2%). streptococci were poorly represented (median value 4.2%) as were anaerobic gram-negative organisms. aerobic gram-negative bacteria were identified as neisseriae and moraxella sp. th ... | 1977 | 270499 |
| the effects of fluoride on the percentage bacterial composition of dental plaque, on caries incidence, and on the in vitro growth of streptococcus mutans, actinomyces viscosus, and actinobacillus sp. | administration of 250 mug/ml naf in drinking water to rats reduced both caries incidence (p less than 0.001) and the percentage of s mutans (0.01 greater than p greater than 0.001) although the percentage of actinobacillus sp. was increased (p less than 0.001). an actinobacillus sp. and s mutans fa1 both proliferated in naf broth only if the naf greater than 10 mug/ml, which was associated with the inhibition of acid production. | 1977 | 272378 |
| sucrose metabolism by prominent members of the flora isolated from cariogenic and non-cariogenic dental plaques. | sucrose metabolism by resting-cell suspensions of pure cultures of representative members of the predominant cultivable flora isolated from cariogenic and non-cariogenic dental plaque was investigated by means of radiochemical techniques. streptocococcus mutans utilized sucrose at a considerably faster rate than s. sanguis, s. mitis, actinomyces viscosus, a. naeslundii, or lactobacillus casei, forming lactic acid, intracellular polysaccharide, insoluble extracellular glucan, and lactic acid from ... | 1977 | 407163 |
| interdependence of in vitro responsiveness of cord and maternal blood lymphocytes to antigens from oral bacteria. | a group of thirty-five mothers and their babies at parturition were examined by the in vitro lymphocyte transformation test to determine sensitization by oral bacterial antigens, b-cell mitogens and dental plaque. lymphocytes from babies of sensitized mothers with gingival or periodontal disease gave the highest frequency (70 and 63%) and magnitude (mean stimulation index of 3.4 and 3.3) of response in cultures stimulated by actinomyces viscosus and veillonella alcalescens. however, igm antibodi ... | 1977 | 606445 |
| are actinomyces viscosus antigens b cell mitogens? | an extracellular heteroglycan (echg) and a sonicated cell supernatant (scs) of actinomyces viscosus ny 1 induced strong lymphocyte proliferation. this was shown with spleen and thoracic duct cells form germfree rats and confirmed with cells from conventional "nude" mouse spleens. spleen cells developed direct plaque-forming cells against densely coupled tnp-srbc. the mitogenic property of echg was diminished considerably after mild alkaline hydrolysis for lymphocytes form rat spleens and was tot ... | 1977 | 300408 |
| complement activation by the alternative pathway and macrophage enzyme secretion in the pathogenesis of chronic inflammation. | a number of stimuli known to induce acid hydrolase secretion from cultured macrophages were examined for their ability to activate c3 via the alternative pathway of the complement system. loss of haemolytically active c3 was checked in normal and c4-deficient guinea-pig serum. for comparison the interactions of cultured macrophages with other agents well known as potent activators of the alternative pathway of the complement system have been investigated. as judged by their activity in these ass ... | 1977 | 328387 |
| origin of esterases in human whole saliva. | whole saliva of 59 healthy persons was used for determination of esterase activity. the pattern of esterase was studied by means of isoelectrofocusing on thin-layer acrylamide gels. the esterases found in whole saliva are suggested to be derived from the cells of the tissue in the oral cavity. this origin is indicated (e.g.) by comparison between isolectrophoretic esterase patterns of whole saliva, submandibular saliva, gingival biopsy and fibroblast culture. antisera against partially purified ... | 1977 | 862601 |
| in vitro evaluation of three iodine-containing compounds as antiplaque agents. | the antibacterial activity of three iodine-containing compounds was determined for strains of four dental plaque-forming microorganisms, using an in vitro preclinical plaque model system. solutions of inorganic iodine, povidone-iodine, and wescodyne were tested for antiplaque activity against preformed plaques of a number of strains of streptococcus mutans, streptococcus sanguis, actinomyces viscosus, and actinomyces naeslundii. solutions of inorganic iodine were more effective as antiplaque age ... | 1977 | 883812 |
| paraplegia due to actinomyces viscosus infection in the cat. | in a 3 year old female domestic cat a suppurative, granulomatous lesion of the tail and sacral area penetrated into the epidural space, causing paraplegia. a viscosus was isolated from the inflammatory tissues. a comparative light and electronmicroscopic study of the bacterial elements and the architecture of the granules (drusen) show that the latter are in-vivo microcolonies of the agent. | 1977 | 906806 |
| regulation of invertase of actinomyces viscosus. | the regulation of diethylaminoethyl-partially purified invertase (ec 3.2.1.26; beta-d-fructofuranoside fructohydrolase) from the 37,000 x g-soluble intracellular fluid of actinomyces viscosus serotype 2 strain m-100 was studied. glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. fructose-6-phosphate (f6p) and fructose-1,6-diphosphate (fdp) were found to act as noncompetitive inhibitors of inver ... | 1977 | 903175 |
| distribution of menaquinones in actinomycetes and corynebacteria. | menaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined. dihydromenaquinones having nine isoprene units were the main components isolated from gordona, mycobacterium, corynebacterium bovis, corynebacterium glutamicum and a strain labelled nocardia farcinica, but dihydromenaquinones having eight isoprene units were characteristic of other corynebacterium species and representatives of the 'rhodochrous' complex. tetrahydromenaquinones having six ... | 1977 | 894261 |
| isolation of actinomyces viscosus from two patients with clinical infections. | the isolation of actinomyces viscosus from two patients is described. one was a case of multiple myeloma, the organism being found on blood culture; the other was a patient with a submandibular abscess. these are believed to be the first such isolations of a. viscosus in this country. | 1977 | 874107 |
| structural requirements of guanide, biguanide, and bisbiguanide agents for antiplaque activity. | the bactericidal efficacy of 16 guanide, biguanide, and bisbiguanide agents was studied in vitro against intact preformed plaques of four oral (plaque-forming) microorganisms: streptococcus mutans, s. sanguis, actinomyces viscosus, and a. naeslundii. the activities of these agents were examined in relation to their molecular configurations. these studies indicated that the bis- and biguanide configurations are important for efficacy, as is the length of the alkyl side chain. no structural moiety ... | 1977 | 931371 |
| interaction of inflammatory cells and oral microorganisms. vii. in vitro polymorphonuclear responses to viable bacteria and to subcellular components of avirulent and virulent strains of actinomyces viscosus. | both virulent (v) and avirulent (av) strains of actinomyces viscosus t14 are capable of colonizing the oral cavity of gnotobiotic rats, but only t14-v causes destructive periodontal disease. the basis for this difference in in vivo pathogenicity has not been adequately defined. in the present study we compared the capacities of t14-av and t14-v to provoke in vitro extracellular release of lysosomal constituents from human polymorphonuclear leukocytes (pmns). in serum-free cultures, viable t14-v ... | 1978 | 689737 |
| inhibitory effects of extracellular products from oral bacteria on human fibroblasts and stimulated lymphocytes. | extracellular products of 12 strains of streptococcus mutans and 5 additional species of oral bacteria were analyzed for their ability to inhibit proliferation of fibroblastoid cells (hela and av3) and blast transformation of human peripheral blood lymphocytes obtained from normal individuals. products from s. mutans strains aht and bht, streptococcus intermedius, and actinomyces viscosus inhibited [3h]thymidine uptake by fibroblastoid cells and phytohemagglutinin-stimulated lymphocytes. product ... | 1978 | 689736 |
| new medium for isolation of actinomyces viscosus and actinomyces naeslundii from dental plaque. | metronidazole (10 microgram/ml) and cadmium sulfate (20 microgram/ml) were added to a gelatin-based medium to select for microaerophilic actinomyces species from dental plaque samples. the new medium (gmc), when incubated anaerobically, allowed 98% recovery of seven pure cultures of actinomyces viscosus and 73% recovery of eight pure cultures of actinomyces naeslundii, while suppressing 76% of the total count of other organisms in dental plaque samples. in 203 plaque samples, recoveries of a. vi ... | 1978 | 670376 |
| isolation of a bacteriophage for actinomyces viscosus. | a lytic phage which produces clear plaques on a human isolate of actinomyces viscosus was isolated from a sample of raw domestic sewage. | 1978 | 669798 |
| comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. | the adsorption of strains of prominent oral bacteria to hydroxyapatite (ha) surfaces was studied by use of an adsorption model based on the langmuir adsorption isotherm; this permitted comparative estimates of the number of adsorption sites and the strength of the adsorption bonds on untreated and salivatreated ha surfaces for strain of streptococcus mutans, s. salivarius, s. sanguis, s. mitis, actinomyces viscosus, and a. naeslundii. the experimental data closely followed the adsorption model a ... | 1978 | 640732 |
| identification of the virulence-associated antigen on the surface fibrils of actinomyces viscosus t14. | actinomyces viscosus t14v is virulent (v) for monoinfected rats, causing periodontal disease and bone loss, whereas, a. viscosus t14av, a mutant strain, is avirulent (av). surface antigens from the t14v and t14av strains were prepared by lysozyme digestion of cell walls and were compared by immunodiffusion against antisera to t14v and t14av whole cells. the v-associated antigen (v-antigen) was detected readily in the t14v, but not readily in the t14av cell wall extract. antiserum specific for th ... | 1978 | 624593 |
| morphological features and functional properties of human fibroblasts exposed to actinomyces viscosus substances. | connective tissue fibroblasts undergo cytopathic degenerative changes during certain long-term inflammatory diseases such as rheumatoid arthritis and periodontitis. the failure of inflamed tissues to repair properly may result from functional alterations of fibroblasts within the affected tissues. numerous previous studies indicate that direct cytotoxicity by bacterial or other substances may be responsible for the cellular alterations observed in vivo. we have tested this hypothesis by exposing ... | 1978 | 624591 |
| [biological activities of a human dental plaque specimen, and cell walls from actinomyces viscosus and actinomyces naeslundii]. | 1978 | 397329 | |
| modification of surface composition of actinomyces viscosus t14v and t14av. | the morphology and serology of actinomyces viscosus t14v and t14av were compared. when grown in supplemented tryptic soy broth, the virulent strain (t14v) possessed an extensive network of cell surface fibrils. in this medium, the avirulent strain (t14av) possessed a microcapsule, absent on strain t14v, and a comparatively small number of surface fibrils. mild acid extraction (lancefield procedure) solubilized common antigenic components on both strains as well as components detectable only in t ... | 1978 | 730385 |
| rapid method for identification and enumeration of oral actinomyces. | serotype-specific antisera prepared against whole cells of actinomyces viscosus, a. naeslundii, and a. israeli were labeled with fluorescein dye and used to detect and quantitate antigenically related microorganisms in human dental plaque. by relating the dna content of the dental plaque microflora to the number of actinomyces present in the plaque samples, a reproducible method was developed for specifically enumerating five serotypic representatives of this genus found in human plaque. | 1978 | 711333 |
| in vitro antiplaque properties of a series of alkyl bis(biguanides). | a series of eight alkyl bis(biguanide) analogues of alexidine, n,n''''-1,6-hexanediyl bis[n'-(2-ethylhexyl)imidodicarbonimidic diamide] (1), was prepared. five of these analogues constituted a series isolipophilic with 1 but with varying bridge length between biguanide moieties. the compounds were evaluated in vitro for antibacterial and antiplaque properties against streptococcus mutans, actinomyces viscosus, and actinomyces naesludii. one analogue, n n,''''-1,6-hexanediyl bis[n'(n-octyl)imidod ... | 1978 | 691009 |
| cell-associated levan of actinomyces viscosus. | actinomyces viscosus atcc 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. levan was not detectable on the surface of glucose-grown a. viscosus, but after a brief incubation of these cells with 5% sucrose, they were encapsulated with tenaciously adhering levan. the levan layer constituted between 0.02 and 0.03% of the cell dry weight. in contrast, sucrose-grown a. viscosus cells possessed a low l ... | 1978 | 344220 |
| quantitative fluorescent immunoassay of antibodies to, and surface antigens of, actinomyces viscosus. | optimal conditions for a fluorescence immunoassay of antibodies to, and surface antigens of, actinomyces viscosus atcc 19246 are described. in the standard fluorescence immunoassay, 10(8) colony-forming units of a. viscosus reacted with an antibody preparation, were washed, and then were treated with an excess of fluorescein-conjugated goat anti-rabbit immunoglobulin g. after another set of washes, fluorescence was determined in a spectofluorometer; in most cases excitation was at 485 nm, with e ... | 1978 | 344340 |
| binding of glucosyltransferase and glucan synthesis by streptococcus mutans and other bacteria. | lyophilized and heat-treated cells from the seven serotypes of streptococcus mutans were examined for their ability to bind added insoluble-product glucosyl-transferase (gtase) and to synthesize cell-associated glucan from [(14)c]sucrose. lyophilized cells of serotypes a and g did not synthesize any more additional glucan than did the controls after exposure to gtase. these cells, however, synthesized four- to eightfold-greater quantities of glucan than did the cells of the remaining serotypes. ... | 1978 | 361564 |
| rat memory t lymphocytes: in vitro proliferation induced by antigens of actinomyces viscosus. | peripheral t lymphocytes from ric-sprague-dawley rats primed in vivo several months earlier with actinomyces viscosus ny 1 reacted with a strong proliferative response upon antigenic challenge in vitro. two different antigen preparations from a. viscosus ny 1, a broken cell supernatant (bcs) and an extracellular fraction (exc) gave maximum responses as measured by the uptake of [3h]thymidine at a concentration of 10 microgram at day 4 of culture. phytohaemagglutinin (pha) used as a control provo ... | 1978 | 306665 |
| the effect of cyclosporin a on dental caries in rats monoassociated with actinomyces viscosus ny 1. | 1978 | 311139 | |
| cervicofacial actinomyces viscosus infection. | 1978 | 712485 | |
| role of colonization in the virulence of actinomyces viscosus strains t14-vi and t14-av. | germfree rats fed a high-sucrose diet were inoculated with actinomyces viscosus strain t14-vi (virulent) or t14-av (avirulent). the mean recovery of strain t14-vi from six extracted finely ground molars of rats sacrificed after 90 days was 1.1 x 10(8) colony-forming units (cfu). the mean recovery of strain t14-av was 5.7 x 10(7) cfu, which was significantly less. strain t14-vi caused severe alveolar bone loss, but only minimal bone loss occurred in rats infected with strain t14-av. scanning elec ... | 1978 | 730375 |
| new cellular and extracellular amphipathic antigen from actinomyces viscosus ny1. | a new type of amphipathic antigen was isolated from culture supernatants and cells of actinomyces viscosus ny1 and partially characterized as a fatty acid-substituted heteropolysaccharide. similar components have been detected serologically in other strains of actinomyces. | 1978 | 730376 |
| the polar lipids of actinomyces viscosus. | 1978 | 273391 | |
| microbiota of gingivitis in man. | a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ... | 1978 | 276916 |
| inhibition of actinomyces viscosus by bacteriocin-producing strains of streptococcus mutans in the dental plaque of gnotobiotic rats. | 1978 | 280286 | |
| relative proportions of actinomyces viscosus and actinomyces naeslundii in dental plaques collected from single sites. | 1978 | 280567 | |
| a comparison of strains of bacteria designated actinomyces viscosus and actinomyces naeslundii. | 1978 | 282008 | |
| the in vitro inhibition of microbial growth and plaque formation by surfactant drugs. | several cationic, mixed and amphoteric surfactants were tested for their antimicrobial activity and ability to inhibit the formation of in vitro plaque by oral microorganisms. all had antimicrobial activity against actinomyces viscosus. actinomyces naeslundii and streptococcus mutans. cationic surfactants were comparable to chlorhexidine in antimicrobial activity but were less effective in inhibiting plaque formation. amphoteric surfactants were less effective than other detergents in antibacter ... | 1978 | 151134 |
| [serological analysis of actinomyces viscosus and identification of pathogenic human strains]. | 1978 | 115912 | |
| fimbriae of actinomyces viscosus t14v: their relationship to the virulence-associated antigen and to coaggregation with streptococcus sanguis 34. | 1) fimbriae from a. viscosus t14v may be similar to those found on other bacteria. 2) the antigenic difference between virulent and avirulent a. viscosus t14 appears to be of a quantitative rather than a qualitative nature and is related to fimbriae and not to the cell wall polysaccharide. 3) coaggregation between a. viscosus t14v and s. sanguis 34 is mediated by fimbriae on the former which have specificity for beta-linked galactosyl residues. | 1978 | 84522 |