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dengue virus type-3 envelope protein domain iii; expression and immunogenicity.production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. the goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice.025691924
construction and characterisation of a complete reverse genetics system of dengue virus type 3.dengue virulence and fitness are important factors that determine disease outcome. however, dengue virus (denv) molecular biology and pathogenesis are not completely elucidated. new insights on those mechanisms have been facilitated by the development of reverse genetic systems in the past decades. unfortunately, instability of flavivirus genomes cloned in escherichia coli has been a major problem in these systems. here, we describe the development of a complete reverse genetics system, based on ...024402142
monoclonal antibodies against ns3 and ns5 proteins of japanese encephalitis virus.non-structural proteins ns3 and ns5 of japanese encephalitis virus (jev) were expressed in escherichia coli and purified by dialysis. two monoclonal antibodies (mabs) named 1h7 and 2d4 against ns3 protein and three mabs named 3c4, 3h7, and 3f10 against ns5 protein were generated by fusing mouse myeloma cell line sp2/0 with spleen lymphocytes from ns3 or ns5 protein immunized mice. then activity of mabs was characterized by enzyme-linked immunosorbent assay (elisa), western blot analysis, and ind ...022509919
development of monoclonal antibodies specifically recognizing the endogenous sterile alpha motif and hd domain 1 protein in porcine cell lines.the sterile alpha motif and hd domain 1 (samhd1) protein has been identified as a novel innate immunity restriction factor that participates in processes crucial to the viral life cycle. in the present study, we describe a procedure to generate monoclonal antibodies (mabs) against porcine samhd1 and investigate its characteristics to analyze the expression of endogenous samhd1. the open reading frame of porcine samhd1 was cloned into the prokaryotic expression vector pcold-tf dna to construct a ...025358004
the lt1 and lt2 variants of the enterotoxigenic escherichia coli (etec) heat-labile toxin (lt) are associated with major etec lineages.the heat-labile toxin (lt) is one of the major virulence factors of enterotoxigenic escherichia coli (etec). we recently described that 20 polymorphic lt variants are present in etec strains isolated globally. two of the variants, lt1 and lt2, are particularly common and we found that they were associated with clonal etec lineages that express the colonization factors (cfs), cfa/i, cs1+cs3, cs2+cs3, and cs5+cs6. etec expressing these cfs are frequently found among etec strains isolated from case ...026939855
molecular characterization of diarrheagenic escherichia coli from libya.diarrheagenic escherichia coli (dec) are important enteric pathogens that cause a wide variety of gastrointestinal diseases, particularly in children. escherichia coli isolates cultured from 243 diarrheal stool samples obtained from libyan children and 50 water samples were screened by polymerase chain reaction (pcr) for genes characteristic of enteroaggregative e. coli (eaec), enteropathogenic e. coli (epec), enterotoxigenic e. coli (etec), enterohemorrhagic e. coli (ehec), and enteroinvasive e ...022556089
evaluation of immunogenicity of novel isoform of eg95 (eg95-5g1) from echinococcus granulosus in balb/c mice.echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. the aim of this study is the production, purification and evaluation immunogenicity of new construction of eg95 protein.025759730
immunogenicity of a fusion protein comprising coli surface antigen 3 and labile b subunit of enterotoxigenic escherichia coli.enterotoxigenic escherichia coli (etec) strains are the major causes of diarrheal disease in humans and animals. colonization factors and enterotoxins are the major virulence factors in etec pathogenesis. for the broad-spectrum protection against etec, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate.025326019
biomechanical and structural features of cs2 fimbriae of enterotoxigenic escherichia coli.enterotoxigenic escherichia coli (etec) are a major cause of diarrhea worldwide, and infection of children in under-developed countries often leads to high mortality rates. isolated etec expresses a plethora of colonization factors (fimbriae/pili), of which cfa/i and cfa/ii, which are assembled via the alternate chaperone pathway (acp), are among the most common. fimbriae are filamentous structures whose shafts are primarily composed of helically arranged single pilin-protein subunits, with a un ...026153701
antioxidative and antimutagenic activities of 70% ethanolic extracts from four fungal mycelia-fermented specialty rices.the health-promoting potential of 70% ethanolic extracts of 4 rice varieties fermented with monascus ruber, phellinus linteus, cordyceps sinensis and agaricus blazei was evaluated mainly focusing on their antioxidative and antimutagenic capacities based on the following parameters: phenolic compound and phytic acid content; inhibitory activity on lipid peroxidation; scavenging activity on dpph radical; suppressing ability on mitomycin c-induced mutagenesis in e. coli cells; and protective effect ...018818745
coverage of diarrhoea-associated escherichia coli isolates from different origins with two types of phage cocktails.eighty-nine t4-like phages from our phage collection were tested against four collections of childhood diarrhoea-associated escherichia coli isolates representing different geographical origins (mexico versus bangladesh), serotypes (69 o, 27 h serotypes), pathotypes (etec, epec, eiec, eaec, vtec, shigella), epidemiological settings (community and hospitalized diarrhoea) and years of isolation. with a cocktail consisting of 3 to 14 t4-like phages, we achieved 54% to 69% coverage against predomina ...024528873
immunoreactivity evaluation of a new recombinant chimeric protein against brucella in the murine model.brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against brucella is desirable. in this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model.027928487
molecular characterization and antibiotic resistance of enterotoxigenic and entero-aggregative escherichia coli isolated from raw milk and unpasteurized cheeses.the aim of this study was to determine the occurrence of enterotoxigenic and enteroaggregative escherichia coli strains and antibiotic resistance of the isolates in raw milk and unpasteurized cheese. out of 200 samples of raw milk and 50 samples of unpasteurized cheeses, 96 and 24 strains of e. coli were isolated, respectively. polymerase chain reaction (pcr) was used to detect the genes encoding heat-stable enterotoxin a (sta), heat-stable enterotoxin b (stb), heat labile toxin (lt) and enteroa ...025568689
recent advances in understanding enteric pathogenic escherichia coli.although escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. pathogenic variants of e. coli (pathovars or pathotypes) cause much morbidity and mortality worldwide. consequently, pathogenic e. coli is widely studied in humans, animals, food, and the environment. while there are many common features that these pathotypes employ to colonize the intestinal mucosa and cause diseas ...024092857
clinical implications of enteroadherent escherichia coli.pathogenic escherichia coli that colonize the small intestine primarily cause gastrointestinal illness in infants and travelers. the main categories of pathogenic e. coli that colonize the epithelial lining of the small intestine are enterotoxigenic e. coli, enteropathogenic e. coli, and enteroaggregative e. coli. these organisms accomplish their pathogenic process by a complex, coordinated multistage strategy, including nonintimate adherence mediated by various adhesins. these so called "entero ...022798032
genome analysis of bovine-mastitis-associated escherichia coli o32:h37 strain p4.escherichia coli is a major pathogen of bovine intramammary infections. here we report the first draft of the genome sequence of the e. coli o32:h37 p4 strain, which is widely used in experimental bovine mastitis studies.022740662
characterization of unstable pentyn10 from enterotoxigenic escherichia coli (etec) o169:h41.enterotoxigenic escherichia coli (etec) serotype o169:h41 has been an extremely destructive epidemic etec type worldwide. the strain harbors a large unstable plasmid that is regarded as responsible for its virulence, although its etiology has remained unknown. to examine its genetic background specifically on the unstable retention and responsibility in the unique adherence to epithelial cells and enterotoxin production, the complete sequence of a plasmid, pentyn10, purified from the serotype st ...026575107
the hepatitis b virus core variants that expose foreign c-terminal insertions on the outer surface of virus-like particles.the major immunodominant region (mir) and n-terminus of the hepatitis b virus (hbv) core (hbc) protein were used to expose foreign insertions on the outer surface of hbc virus-like particles (vlps). the additions to the hbc positively charged arginine-rich c-terminal (ct) domain are usually not exposed on the vlp surface. here, we constructed a set of recombinant hbcg vectors in which ct arginine stretches were substituted by glycine residues. in contrast to natural hbc vlps and recombinant hbc ...026446016
preparation and characterization of a monoclonal antibody against the core protein vp7 of the 25th serotype of bluetongue virus.bluetongue virus (btv) is a member of the genus orbivirus, within the family reoviridae. the vp7 protein of btv is used for developing group-specific serological assays. to prepare monoclonal antibody (mab) against vp7 of the 25th serotype btv, the rna s7 encoding vp7 was cloned into prokaryotic expression vectors pet-28a (+) and pgex-6p-1 to generate recombinant plasmids. the recombinant protein vp7 was expressed in escherichia coli bl21 (de3), respectively. the results of sds-page revealed tha ...025897610
preparation of egg yolk antibodies against bont/b and their passive protection in mouse models.botulism in human is a devastating intoxication caused mainly by type a, b, and e botulinum neurotoxins (bonts). the most effective treatment of botulism is injection of bont antiserum in the first 24 h. in this study, a recombinant c-terminal heavy chain of bont/b (bhc) was successfully expressed in e. coli. the soluble bhc was used as an antigen to immunize laying hens for yolk immunoglobulin (igy) production. the purified igy against bhc subunit, preincubated with the bont/b, was predominantl ...025424938
etiology of acute diarrhea in tunisian children with emphasis on diarrheagenic escherichia coli: prevalence and identification of e. coli virulence markers.diarrheal diseases can be caused by viral, bacterial and parasitic infections. this paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in tunisian children less than five years of age.025909062
overcoming enterotoxigenic escherichia coli pathogen diversity: translational molecular approaches to inform vaccine design.enterotoxigenic escherichia coli (etec) are a genetically diverse e. coli pathovar that share in the ability to produce heat-labile toxin and/or heat-stable toxins. while these pathogens contribute substantially to the burden of diarrheal illness in developing countries, at present, there is no suitable broadly protective vaccine to prevent these common infections. most vaccine development attempts to date have followed a classical approach involving a relatively small group of antigens. the ext ...027076141
development of an anti-elicitin antibody-based immunohistochemical assay for diagnosis of pythiosis.pythiosis is an emerging and life-threatening infectious disease of humans and animals living in tropical and subtropical countries and is caused by the fungus-like organism pythium insidiosum. antifungals are ineffective against this pathogen. most patients undergo surgical removal of the infected organ, and many die from advanced infections. early and accurate diagnosis leads to prompt management and promotes better prognosis for affected patients. immunohistochemical assays (ihcs) have been d ...026719582
gut microbiota elicits a protective immune response against malaria transmission.glycosylation processes are under high natural selection pressure, presumably because these can modulate resistance to infection. here, we asked whether inactivation of the udp-galactose:β-galactoside-α1-3-galactosyltransferase (α1,3gt) gene, which ablated the expression of the galα1-3galβ1-4glcnac-r (α-gal) glycan and allowed for the production of anti-α-gal antibodies (abs) in humans, confers protection against plasmodium spp. infection, the causative agent of malaria and a major driving force ...025480293
hypoallergenic derivatives of fel d 1 obtained by rational reassembly for allergy vaccination and tolerance induction.the major cat allergen fel d 1 represents one of the most important respiratory allergens. aim of this study was to engineer recombinant fel d 1 derivatives with reduced ige reactivity and preserved t cell epitopes for vaccination and tolerance induction.024552249
t cell activation status determines the cytokine pattern induced by zymosan and bacterial dna both in thymocytes and splenocytes.proinflammatory cytokines are essential mediators of the immunopathology associated with microbial sepsis. the fungal cell wall component zymosan and bacterial dna are well-studied experimental tools for investigating these processes, simulating the presence of fungal or bacterial infection. cells of the immune periphery, but also immune cells in the thymus, are affected essentially by the presence of microbes or their immune stimuli in sepsis. for this reason, we investigated the cytokine patte ...023574321
escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in iran.diarrheagenic escherichia coli have developed different strategies for establishment of infection in their host. understanding these pathogenic mechanisms has led to the development of specific diagnostic tools for identification and categorization of e. coli strains into different pathotypes. this review aims to provide an overview of the various categories of diarrheagenic escherichia coli and the data obtained in iran pertaining to these pathotypes.023066484
discovery and characterization of two nimrod superfamily members in anopheles gambiae.anti-bacterial proteins in mosquitoes are known to play an important modulatory role on immune responses to infections with human pathogens including malaria parasites. in this study we characterized two members of the anopheles gambiae nimrod superfamily, namely agnimb2 and ageater. we confirm that current annotation of the an. gambiae genome incorrectly identifies agnimb2 and ageater as a single gene, agap009762. through in silico and experimental approaches, it has been shown that agnimb2 is ...024428830
edema and tetraparesis in a miniature pig after allogeneic hematopoietic cell transplantation.a 3-mo-old, 12-kg, intact, miniature pig presented with severe neurologic signs on day 8 after hematopoietic cell transplantation. this pig had received an immunosuppressive regimen before transplantation that included an anticd3 immunotoxin for t-cell depletion, 100 cgy of total-body irradiation, and cyclosporine for 45 d. the pig began exhibiting erythematous lesions on posttransplantation day 7. he also demonstrated increased conscious proprioceptive deficits and recumbency but normal mentati ...023043783
cloning and sequence analysis of wild argali isg15 cdna.the complete coding sequence of wild argali isg15 cdna was generated by rapid amplification of cdna ends. the isg15 cdna was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kda protein composed of 157 amino acids. its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of isg15cdna from ovis aries (accession no. nm001009735.1), capra hircus (accession no. hq329186.1), bos taurus (accession no. bc102318.1), bubalus bubalis (accession no. hm543269.1) ...025049988
bacterial expression strategies for several sus scrofa diacylglycerol kinase alpha constructs: solubility challenges.we pursued several strategies for expressing either full-length sus scrofa diacylglycerol kinase (dgk) alpha or the catalytic domain (alphacat) in escherichia coli. alphacat could be extracted, refolded, and purified from inclusion bodies, but when subjected to analytical gel filtration chromatography, it elutes in the void volume, in what we conclude are microscopic aggregates unsuitable for x-ray crystallography. adding glutathione s-transferase, thioredoxin, or maltose binding protein as n-te ...023558375
hydrophilic/hydrophobic characters of antimicrobial peptides derived from animals and their effects on multidrug resistant clinical isolates.multidrug resistant (mdr) pathogen infections are serious threats to hospitalized patients because of the limited therapeutic options. a novel group of antibiotic candidates, antimicrobial peptides (amps), have recently shown powerful activities against both gram-negative and gram-positive bacteria. unfortunately, the viability of using these amps in clinical settings remains to be seen, since most still need to be evaluated prior to clinical trials and not all of amps are potent against mdr cli ...025730460
structural comparison of bacterial multidrug efflux pumps of the major facilitator superfamily.the biological membrane is an efficient barrier against water-soluble substances. solute transporters circumvent this membrane barrier by transporting water-soluble solutes across the membrane to the other sides. these transport proteins are thus required for all living organisms. microorganisms, such as bacteria, effectively exploit solute transporters to acquire useful nutrients for growth or to expel substances that are inhibitory to their growth. overall, there are distinct types of related ...027065631
identification of a heat shock cognate protein 70 gene in chinese soft-shell turtle (pelodiscus sinensis) and its expression profiles under thermal stress.the heat shock cognate protein 70 (hsc70) is a member of a 70-kda heat shock protein (hsp70) family that functions as molecular chaperones. in this study, a novel hsc70 gene from chinese soft-shelled turtle (pelodiscus sinensis) (thsc70) was identified. the thsc70 full-length complementary dna (cdna) is 2272 bp long with a 1941-bp open reading frame (orf) encoding 646 amino acids. three characteristic signature regions of the hsp70 family, two major domains of an adenosine triphosphate (atp)/gua ...022661209
molecular cloning and characterization of a new c-type lysozyme gene from yak mammary tissue.milk lysozyme is the ubiquitous enzyme in milk of mammals. in this study, the cdna sequence of a new chicken-type (c-type) milk lysozyme gene (yml), was cloned from yak mammary gland tissue. a 444 bp open reading frames, which encodes 148 amino acids (16.54 kda) with a signal peptide of 18 amino acids, was sequenced. further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. recombinant yak milk lys ...026580446
recombinant goat vegf164 increases hair growth by painting process on the skin of shaved mouse.to detect goat vascular endothelial growth factor (vegf)-mediated regrowth of hair, full-length vegf164 cdna was cloned from inner mongolia cashmere goat (capra hircus) into the pet-his prokaryotic expression vector, and the recombinant plasmid was transferred into e. coli bl21 cells. the expression of recombinant 6×his-gvegf164 protein was induced by 0.5 mm isopropyl thio-β-d-galactoside at 32°c. recombinant goat vegf164 (rgvegf164) was purified and identi ed by western blot using monoclonal an ...025178380
iii. the importance to the surgeon of familiarity with the bacillus coli communis. 189317859963
the influence of acute alcoholism on the normal vital resistance of rabbits to infection.the deductions that may be drawn from the results of these experiments are as follows: that the normal vital resistance of rabbits to infection by streptococcus pyogenes (erysipelatos) is markedly diminished through the influence of alcohol when given daily to the stage of acute intoxication. that a similar, though by no means so conspicuous, diminution of resistance to infection and intoxication by the bacillus coli communis also occurs in rabbits subjected to the same influences. and that, whi ...189619866807
the influence of environment upon the biological processes of the various members of the colon group of bacilli : an experimental study.assuming the typical colon bacillus and the typical typhoid bacillus to represent the types of this group that present the greatest divergences in biological peculiarities, we conclude, as others also have done, that there is a series of closely related forms that may be regarded as intermediate or transitional and which serve to establish a biological relationship, either near or remote, between these two typical members. from our own studies we are inclined to regard the typical colon bacillus ...189719866851
note on the specific action of normal human serum upon the bacillus coli communis. 189820757541
the relation of bacillus coli communis to other organisms in the urine. 189920758739
on the detection of bacillus coli communis in water. 190019971299
the use of carbolic acid in isolating the bacillus coli communis from river water. 190119971378
some theoretical considerations upon the nature oe agglutinins, together with further observations upon bacillus typhi abdominalis, bacillus enteritidis, bacillus coli communis, bacillus lactis aerogenes, and some other bacilli of allied character. 190119866950
the relative abundance of bacillus coli communis in river water as an index of the self-purification of streams. 190120474123
the significance of bacillus coli in drinking water. 190220474151
bacillus coli communis. 190219601072
on the practical value of presumptive tests for bacillus coli in water. 190219601076
experiments with the neutral-red test for bacillus coli in the examination of water supplies. 190219601077
on some cultural relations and antagonisms of bacillus coli and houston's sewage streptococci; with a method for the detection and separation of these micro-organisms in polluted water. 190319601139
note on the occurrence on grain of organisms resembling the bacillus coli communis. 190517837535
organisms on the surface of grain, with special reference to bacillus coli. 190517731390
notes on bacillus coli communis in drinking-water. 190619971748
contribution to the differentiation of bacillus coli communis from allied species in drinking water. 190819971822
certain infections of the urinary tract, with special reference to tubercle, gonococcus, and bacillus coli communis. 190820763801
the gas production of bacillus coli. 190919971909
an unusual case of appendix abscess due to the pneumococcus and bacillus coli communis: treatment by double vaccine: recovery. 190920764439
empyemata associated with the bacillus coli: treatment by antitoxin. 190920764580
v. infections of the urinary tract due to bacillus coli and allied organisms: further observations on the treatment of acute and chronic conditions. 191017862501
bacillus coli infections, with special reference to their recognition and comparative frequency. 191020765303
on the varieties of bacillus coli associated with the house-fly (musca domestica). 191120474463
a note on penfold's modification of bacillus coli communis. 191317732678
the production of acetaldehyde during the anaerobic fermentation of glucose by bacillus coli communis (escherich). 191316742256
a study of cultures from sputum and blood in lobar pneumonia.in the thirty-two unselected cases studied, cultures were taken from both the blood and the sputum, and in five of these more than one blood culture was taken. in one case the culture was taken from the blood only, and in this instance the pneumococcus was found. this case will be considered only with reference to the incidence of the pneumococci in blood cultures in lobar pneumonia. the results in the thirty-two cases in which cultures were made both from the blood and the sputum are as follows ...191319867641
the cultivation of amoebae in pure culture upon autolyzed tissues.since the purpose of this paper is to record the cultivation of amoebae upon autolyzed tissue without bacterial association, the morphological characteristics, life cycle, means of differentiating species, and pathogenicity of the protozoa have been omitted. these subjects will be considered in later publications. the result of this study proves that some species of amoebae from liver abscesses and the human intestine can be cultivated upon various autolyzed tissues of man and some of the lower ...191319867701
the effect of storage in river water (sterilized) on the production of acid in carbohydrate solutions by the bacillus coli group. 191417796823
the reducing enzyme of bacillus coli communis. 191516742385
the reactions between bacteria and animal tissues under conditions of artificial cultivation.this report gives an outline of the results of observations on over 1,100 tissue cultures made during the fall, winter, and spring of 1913 to 1914. the work has been resumed in the fall of 1914 and will be continued along the same and allied lines, confirming the above results with other strains of the same organisms and with other bacteria and bacterial products. tests should be made with tissue and plasma from other animals, and the known pathogenicity of the organism for the animal and tissue ...191519867854
a comparison of the acid production of the bacillus coli group isolated from various sources. 191618009389
the reactions between bacteria and animal tissues under conditions of artificial cultivation : ii. bactericidal action in tissue cultures.the results here reported confirm those of the former papers and strengthen the conclusions drawn therefrom. they may be summarized as follows: chicken plasma has a marked bactericidal action on bacillus typhosus, which may be in some slight degree overcome by the presence of growing tissue, especially splenic tissue, in the cultures. on bacillus dysenterioe this bactericidal action of chicken plasma is present, but much less marked, and the same counteracting action of tissue, especially spleni ...191619867985
the production of amyloid disease and chronic nephritis in rabbits by repeated intravenous injections of living colon bacilli.the repeated intravenous injection of rabbits with living bacillus coli communior over long periods has resulted in the formation of amyloid deposits in the spleen, liver, and kidneys. suppurative lesions were not present in most cases and therefore not a factor in its production. the results have been constant in that amyloid was found in all rabbits, eight in number, which were injected over a period of 88 days or more. eight rabbits showed amyloid in the spleen, six of these in the kidneys al ...191619868022
the latent period in the growth of bacteria.1. cultures of diplococcus pneumoniae, bacillus coli, bacillus fluorescens liquefaciens and bacillus prodigiosus, when grown in meat infusion broth exhibit an initial latent period when the culture used for inoculation is no longer growing at its maximum rate; if, however, the culture is growing at its most rapid rate the bacteria, upon subculture, show no latent period but continue to multiply at the same rate as that of the parent culture. 2. if broth cultures of pneumococcus are centrifuged a ...191619868050
bacillus coli in milk-a preliminary report. 191718009653
method of isolating and identifying bacillus coli used at the cincinnati filtration plant. 191718009790
studies in glomerulonephritis : ii. a form of acute glomerulonephritis produced with diphtheria toxin and bacillus coli.1. rabbits in which a mild grade of glomerular damage has been produced by small doses of diphtheria toxin develop a severe glomerulonephritis corresponding to the extracapillary form described by volhard and fahr after inoculations with sublethal quantities of bacillus coli. 2. similar, though not completely identical lesions are produced when diphtheria toxin is followed by intravenous injections of vaughan's split protein (poisonous part) of bacillus coli. 3. the inference is drawn that under ...191719868142
studies in glomerulonephritis : iii. an attempt to produce glomerulonephritis by repeated injections of bacteria.repeated injections into the blood stream of streptococci and stapnylococci derived from cases of scarlet fever, and of bacillus coli communior failed to produce typical glomerulonephritis even when immune antibodies could be demonstrated in the serum in high dilutions. bacteriolysis of streptococci was not found by the usual tests in intro or by the pfeiffer procedure. it is therefore concluded that the weight of evidence is against the theory that glomerulonephritis is due to immune bacterioly ...191719868177
the biological identity of the friedlander bacillus.we conclude therefore that this series of eleven lactose-negative organisms of the friedländer type, grouped together by perkins on the basis of fermentation reactions represents a single biological group. it can be distinguished from bacillus aerogenes and other similar bacilli by cultural, fermentative, and serological reactions. there appears to be a close analogy between this group and pneumococcus mucosus in the possession of a fixed cultural type, and the behavior toward immune serum. both ...191719868181
the effect of acids on the growth of bacillus coli. 191816742771
a study of the antiseptic properties of certain organic compounds.this study of the inhibitive effect of aniline and some of its derivatives and of the triphenylmethane dyes on certain bacteria warrants the following tentative conclusions: 1. the composition and reaction of the medium exert a marked influence on the behavior of the antiseptic. the higher the concentration of organic nitrogenous compounds (peptone) in the medium, the lower is the effective concentration of the dye. the reaction of the medium modifies the specific action of the antiseptic, owing ...191819868219
acid production graphically registered as an indicator of the vital processes in the cultivation of bacteria.we may formulate the results as follows: 1. bacillus paratyphosus in lactose-peptone-water causes a fall in the curve. the explanation of this must be left to later investigations. 2. bacillus paratyphosus in glucose-peptone-water shows at the beginning a rise similar to that produced by coli, but a fall in the curve follows. 3. if bacillus coli is added to a 24 hour paratyphosus lactose culture a very long latent period is obtained before the formation of acid by coli becomes apparent, presumab ...191819868274
studies in bovine mastitis : iii. injection of the udder with micrococci and other microorganisms.aside from the streptococci, micrococci have been the next most frequent group of organisms isolated from inflamed udders. they produce various types of disease. some give rise to only a mild catarrh of the larger milk ducts and cystern, while others produce more or less severe parenchymatous inflammation. on the whole, the prognosis is more favorable with micrococcic infection than with that associated with streptococci. cases of considerable severity have, however, been attributed to staphyloc ...191819868290
two cases of abscess of testis due to bacillus coli. 191820768922
the value of presumptive tests for bacillus coli based on the routine use of lactose bile and lactose broth. 191916558856
the effects of acids, alkalies, and sugars on the growth and indole formation of bacillus coli: a report to the medical research committee. 191916742836
bacillus coli infection of the kidney. 191920311360
inhibitory action of paratyphoid bacilli on the fermentation of lactose by bacillus coli. i.bacteria of the paratyphoid group may be divided into two classes according to the behavior of 4 day cultures in lactose bouillon after a second inoculation with certain types of bacillus coli. one class includes all true hog-cholera bacilli, the other nearly all true paratyphoid and enteriditis types. under the imposed conditions bacillus coli produces the usual amount of gas in the presence of the first group. in the presence of the second no gas or only a bubble appears. the production of aci ...192019871844
on the amount of heat liberated by bacillus coli when grown in the presence of free amino-acids. 192116993500
lactose broth for isolating bacterium coli from water.these authors suggest simplification of the technic by reducing the lactose content by addition of gentian violet, also pasteurization in place of autoclaving. the announced value of gentian violet for preliminary enrichment of colon cultures is sustained, with cleaner plates and readier identification of colonies. gentian violet has also advantages in ensuring keeping qualities in the broth.192118010454
influence of peptone on indol formation by bacillus coli."of the making of peptone in varieties there is no end," might be the summing up of this paper. the author shows that the different kinds may give negative, weak or strong reactions for indol with a typical strain of b. coli. he suggests testing every lot of peptone, but the principle is evident, that this important product needs standardization.192118010560
chilomastix mesnili and a method for its culture.1. cases of flagellate infection of man by chilomastix mesnili have been reported from nearly every locality in the world. they are fairly abundant in the united states and are often confused with cases of infection by trichomonas intestinalis. 2. the shape of chilomastix is pyriform. the body contains the following structures: nucleus, centrosome, primary, secondary, and tertiary blepharoplasts, parabasal, parastyle, peristomal fiber, ventral cytostome, cytostomal flagellum, three anterior flag ...192119868485
studies on the d'herelle phenomenon.the inhibition produced by the lytic agent on the growth of bacillus coli is greatly influenced by the reaction of the medium; it is faint in a slightly acid (ph 6.8) or neutral (ph 7) or even slightly alkaline broth (ph 7.4), but is much stronger in a more alkaline medium (ph 8 or 8.5). we have isolated from the original strain of bacillus coli two types of organisms; one (type s) is sensitive to the lytic agent, the other (type r) is much more resistant. these types are distinguished also by o ...192119868540
on bacillus coli infections of the urinary tract, especially in relation to haemolytic organisms. 192120474731
certain limitations of the bacillus coli method in water examinations. 192220474766
on bacillus coli infections of the urinary tract especially in relation to haemolytic organisms. 192220474772
the twort-d'herelle phenomenon : ii. lysis and microbic variation.1. when the few individuals still alive in a dissolved culture of bacillus coli are transplanted on slanted agar, a culture results which possesses new characteristics. first observed by bordet and ciuca, this culture received the temporary name of modified coli. in the study described above, we found that this modified coli is very heterogeneous and that its three principal characteristics, resistance to lysis, lysogenic properties, and mucoid growth, are shared among different types of organis ...192219868606
cow serum as a substitute for colostrum in new-born calves.the serum of a normal lactating cow when injected into calves a few hours after birth saved only two out of five calves so treated. serum added to the milk of the first two meals saved three out of five. when the two methods were combined and the serum was both injected and fed all five calves so treated survived as normal calves. these figures to be significant should be compared with the controls of both series. since the beginning of this investigation twelve out of thirteen colostrum-fed cal ...192219868687
on the variation of bacterium coli. 192316558993
studies on salt action: viii. the influence of calcium and sodium salts, at various hydrogen ion concentrations upon the viability of bacterium coli. 192316558995
observations on the viability of the bacterium coli group under natural and artifical conditions. 192318010911
distribution of bacterium coli in feces. 192318011055
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