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broiler chickens as potential source of campylobacter infections in humans.of 46 broiler chickens from a live poultry market in new york city, 38 (83%) harbored campylobacter fetus subsp. jejuni in their rectal flora. the observed mean number of c. fetus per g of feces was 4.4 x 10(6). the organisms survived in the feces for at least 96 h at 4 degrees c whether stored in the gut or transferred to a vial. the best survival medium for pure cultures of c. fetus subsp.jejuni was heart infusion broth supplemented with sterile blood and kept in a microaerophilic atmosphere.19807381018
gastroenteritis due to campylobacter jejuni in riyadh, saudi arabia.during one year, 1452 specimens of faeces from patients with diarrhoea were investigated for campylobacter jejuni. this organism was isolated from nine specimens only (0.6%). none was isolated from 160 control subjects who did not have diarrhoea. though the number of isolates is small, diarrhoea due to c. jejuni was found to be more common in males; the ratio of males to females was 7:2. out of the nine patients, only four were saudis. this indicates that campylobacter gastroenteritis exists in ...19817324103
enteropathogenic bacteria in frozen chicken.eighty-two samples of frozen chicken from retail stores were examined for the presence of campylobacter, yersinia enterocolitica, and salmonellae. aerobic plate counts and numbers of coliform bacteria at 37 degrees c were determined. campylobacter fetus subsp. jejuni was found in 22% of the samples, y. enterocolitica was found in 24.5% and salmonella typhimurium was found in one sample (1.2%). the isolated strains of y. enterocolitica belonged to serotypes 4, 5b, 6, and 8. aerobic plate counts a ...19817020597
incidence of campylobacter jejuni in fresh eviscerated whole market chickens. 19817296414
experimental campylobacter diarrhea in chickens.an animal model for campylobacter fetus subsp. jejuni enteritis was developed in 3-day-old chickens. diarrhea was induced in 88% (22 of 25) of chickens inoculated with 9 x 10(7) bacteria given orally. the mean incubation time was 45 h (range, 24 to 72 h). considerable weight loss was observed in the experimental group compared with the control group. ninety bacteria was the minimal infective dose capable of inducing diarrhea in 90% of the chickens. overall mortality was 32% (8 of 25). light micr ...19817298187
the occurrence of campylobacter jejuni in fresh food and survival under different conditions.campylobacter jejuni was an almost regular finding in chickens and in minced meat from pigs and cattle sold in ordinary food stores. the bacteria survived on the food at 4 degrees c for one week and frozen at -20 degrees c for three months. none of the strains tested survived heat treatment at 60 degrees c for longer than 15 min. c. jejuni is apparently a frequent guest in most kitchens. correct food handling and heat treatment to at least 60 degrees c for 15 min should be enough to prevent infe ...19817310124
occurrence of campylobacter fetus subsp. jejuni and yersinia enterocolitica in domestic animals and in some foods of animal origin in finland.fecal samples of some domestic animals were investigated for occurrence of c. fetus subsp. jejuni and y. enterocolitica. the findings in this survey suggest, that the contamination rate in domestic animals for both bacteria is low. raw milk, pasteurized milk or ground meat samples were not positive for c. fetus subsp. jejuni. four per cent of the raw milk samples were positive for y. enterocolitica. each of the y. enterocolitica strains belonged to nilehn's biotype 1 and they were noninvasive.19817329785
isolation of campylobacter jejuni from poultry carcases.campylobacter jejuni was isolated from 94% of chicken carcase rinses collected in a queensland poultry abattoir. direct plating resulted in the isolation of the organism from 84% of the rinses. a further 10% were found to contain c. jejuni by a procedure of selective enrichment and membrane filtration. counts of c. jejuni on the carcases ranged from 0 to 1.1 x 10(5) organisms per carcase.19817342935
carriage of campylobacter jejuni in healthy and diarrheic animals.feces from normal and diarrheic animals were cultured for campylobacter jejuni. a clear difference could not be detected in carriage between normal and diarrheic cattle, horses, pigs, and dogs. too few diarrheic goats, sheep, and rabbits were sampled for conclusions to be made. carriage rates (%) detected in normal animals were as follows: ducks 88.3, chickens 23.8, sheep 13.6, rabbits 11.3, goats 2.7, cattle 2.5, and dogs 0.5. the organism was not isolated from horses and mink. carriage rates v ...19817224312
antimicrobial susceptibility of campylobacter jejuni isolated from humans with diarrhoea and from healthy chickens. 19817275880
effect of recovery medium on the isolation of campylobacter jejuni before and after heat treatment. 19827180785
serotyping of campylobacter jejuni by slide agglutination based on heat-labile antigenic factors.a serotyping scheme for campylobacter jejuni was developed based on slide agglutination of live bacteria with whole cell antisera absorbed with homologous heated and heterologous unheated cross-reactive antigens. among 815 isolates from human and nonhuman sources, 21 serogroups were recognized. of the 615 isolates from human cases of gastroenteritis, 529 (86%) were typable; 455 strains agglutinated in 20 single antisera, whereas 74 isolates agglutinated in various pairs of antisera, allowing sub ...19827096555
intestinal colonization and competitive exclusion of campylobacter fetus subsp. jejuni in young chicks.colonization of campylobacter fetus subsp. jejuni was investigated in monoxenic and holoxenic chicks. in monoxenic chicks, major colonization was found in the crop and ceca, with populations in the ceca consistently reaching 10(9) colony-forming-units/ml of cecal contents over the 28-day test period. bacteremia was found in most chicks, but no significant gross pathological lesions were detected. in holoxenic chicks, major colonization occurred only in the ceca, and no evidence of bacteremia was ...19827150145
campylobacter jejuni contamination of slaughtered chickens.gut samples from 50 nonselected slaughtered chickens were obtained in two poultry processing plants and cultured for campylobacter jejuni and salmonella. positive results were obtained in 84% and 4%, respectively. viable c. jejuni and salmonella were detected in every phase of processing, even during packaging for commercial purposes. of surface samples taken from 118 slaughtered chickens prepared for delivery to consumers, 88 were contaminated by c. jejuni and 17 by salmonella.19827168372
campylobacter jejuni: incidence in processed broilers and biotype distribution in human and broiler isolates.campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in sydney, australia. three of four flocks examined carried c. jejuni. eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes.19827201780
campylobacter jejuni survival in chicken meat as a function of temperature.recognition of campylobacter fetus subsp. jejuni (referred to hereafter as c. jejuni) as an important human pathogen and its isolation from meat products indicate the need for knowledge of its survival characteristics in meats. thermal death times (d-values) for a single strain and a five-strain composite were determined in 1% peptone and autoclaved ground chicken meat at temperatures ranging from 49 to 57 degrees c. survival was determined for these strains in chicken meat at 4, 23, 37, and 43 ...19826812501
epidemiological aspects of campylobacter jejuni enteritis.an epidemiological study on campylobacter jejuni enterocolitis was performed in an urban swedish community. the study included 55 patients gathered during a six-month period. forty-one of the 55 patients (75%) were infected outside sweden. campylobacter enterocolitis was rare among children within the country. patients infected in sweden had eaten chicken significantly more often than a corresponding control group. seven out of nine chicken consuming campylobacter patients also had prepared the ...19827097000
recovery of campylobacter jejuni and campylobacter coli from inoculated foods by selective enrichment.a direct enrichment procedure was developed to selectively recover small numbers of campylobacter jejuni, c. coli, and nalidixic acid-resistant thermophilic campylobacter from foods. the procedure includes an enrichment medium composed of brucella broth, 7% lysed horse blood, 0.3% sodium succinate, 0.01% cysteine hydrochloride, vancomycin (15 micrograms/ml), trimethoprim (5 micrograms/ml), polymyxin b (20 iu/ml), and cycloheximide (50 micrograms/ml) that is inoculated with 10 or 25 g of food and ...19827103488
pathogenicity of campylobacter jejuni isolates from animals and humans.fourteen isolates of campylobacter jejuni of different serotypes and one campylobacter coli isolate, from various human and animal sources, were tested for potential pathogenic mechanisms. enterotoxin production was not detected in the infant mouse test or by calf and piglet ligated intestinal loop studies. isolates were not invasive by the sereny test. all isolates associated with and penetrated hela cells, although both actions occurred generally in a minor way under the conditions of our stud ...19827141696
prevalence of campylobacter jejuni in two california chicken processing plants.two federally inspected california chicken processing plants participated in campylobacter jejuni prevalence studies. twelve sampling sites were included in each of four groups. groups were based on bird age, scald water temperature, and plant sampled. scald water temperatures of 60 degrees c (140 degrees f) did not contribute to a lower prevalence of c. jejuni in edible parts, as did temperatures of 53 degrees c (127 degrees f) and 49 degrees c (120 degrees f). the feather picker and chilling t ...19836830212
house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni.a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ...19836830213
prevalence of campylobacter jejuni in chicken wings.campylobacter jejuni was found in 82.9% of 94 chicken wing packages analyzed on the day of arrival at supermarkets and in 15.5% of 45 packages obtained from the supermarket shelves a few days later. the number of bacterial cells ranged from 10(2) to 10(3.9) per wing. the prevalence of c. jejuni in the wings varied with the brand, the day of sampling, and the age of the product.19836847181
isolation and enumeration of campylobacter jejuni from poultry products by a selective enrichment method.a direct selective enrichment procedure was developed for the isolation of campylobacter jejuni from poultry products. the selective enrichment medium (atb) consisted of (per liter) tryptose (20 g), yeast extract (2.5 g), sodium chloride (5 g), fbp supplement (ferrous sulfate [0.25 g], sodium metabisulfite [0.25 g], sodium pyruvate [0.25 g]), bicine (10 g), and agar (1 g). hematin solution (6.25 ml; prepared by dissolving 0.032 g of bovine hemin in 10 ml of 0.15 n sodium hydroxide solution and a ...19836651294
campylobacter jejuni and campylobacter coli serotypes isolated from chickens, cattle, and pigs.a total of 191 campylobacter jejuni and 125 campylobacter coli were isolated from the intestinal content of 398 chickens, 421 cattle, and 203 pigs. all 108 chicken isolates and 73 of 80 cattle isolates were c. jejuni, but 115 of the 118 pig isolates were c. coli. a total of 84% of the c. jejuni and 64% of the c. coli isolates were typed on the basis of thermostable antigens with 20 antisera prepared against frequently occurring serotypes in campylobacter enteritis in man (15 c. jejuni, 6 c. coli ...19836630466
pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model.pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ...19836650962
[campylobacter jejuni, yersinia enterocolitica and salmonella in game and poultry].samples of various sorts of game and poultry (other than chicken), obtained from poulterers, hunters, rearing-farms and slaughterhouses, were surveyed for the presence of campylobacter jejuni, yersinia enterocolitica and salmonella. these pathogens were isolated from hare, wild boars, pheasants, guinea-fowl, turkeys, mallards and domestic ducks. yersinia enterocolitica and salmonella were also cultured from wild and domestic rabbits. moreover, yersinia enterocolitica was isolated from wood pigeo ...19836648946
handling raw chicken as a source for sporadic campylobacter jejuni infections. 19836631070
[isolation of campylobacter jejuni from chicken feces and their survival period in minced chicken]. 19836421960
location of campylobacter jejuni in infected chicken livers.to determine whether chicken livers infected with campylobacter jejuni are seeded in vivo or contaminated after slaughtering, 117 livers purchased in retail outlets in new york were examined for surface and tissue infections. of 56 livers positive for c. jejuni, 36 yielded surface growth only, 18 both surface and tissue growth, and 2 tissue growth only. the scanty growth from tissue samples suggests a carry-over of organisms from the surface. it was concluded, therefore, that contamination is mo ...19836863511
effect of temperature, duration of incubation, and ph of enrichment culture on the recovery of campylobacter jejuni from eviscerated market chickens.to establish an enrichment system of high efficiency for recovery of campylobacter jejuni from market chickens, the effects of the temperature, duration of incubation, and ph of the enrichment culture on the isolation of the bacterium were evaluated. whole chickens or chicken parts in plastic bags were individually rinsed, and the washings filtered through cheesecloth. the cells were separated from the washings by centrifugation, and the pellet was inoculated into 100 ml of enrichment broth. iso ...19836684497
endemic campylobacter jejuni infection in colorado: identified risk factors.a study of persons with laboratory-confirmed sporadic campylobacter jejuni infection and of controls matched for age and sex in colorado in the summer of 1981 yielded odds ratio estimates significantly greater than 1 for the following risk factors: drinking raw water (10.74), drinking raw milk (6.93), eating undercooked chicken (2.77), and living in a household with a cat (3.21).19846696155
campylobacter jejuni diarrhea model in infant chickens.to study the pathogenic mechanisms of campylobacter jejuni infection, 36- to 72-h-old chickens were fed 10(3) to 10(6) live cells, using strains isolated from 40 patients with watery diarrhea and 6 with bloody mucoid diarrhea from whom no other known enteropathogen was detected. chickens of starbro strain were more likely to develop c. jejuni-induced diarrhea than were white leghorn chickens. diarrhea was defined on the basis of amounts of gut fluid in 288 chicks fed with live c. jejuni versus 1 ...19846698612
comparative studies on competitive exclusion of three isolates of campylobacter fetus subsp. jejuni in chickens by native gut microflora.resistance of young chicks to campylobacter fetus subsp. jejuni was substantially increased by early exposure to native gut microflora. protection was demonstrated against two human isolates and a chicken isolate of c. fetus subsp. jejuni. significant protection against the chicken isolate was observed throughout a 91-day test period. infection reached 100% (25/25) in the untreated group at 56 days of age and only 4% (1/25) in the group treated with native gut microflora. campylobacter fetus sub ...19846721792
a study of the spread of campylobacter jejuni in four large kitchens.campylobacters were sought in swabs taken from work surfaces, sinks and floors of four kitchens-i.e. hospital, university, cook-freeze and commercial, processing frozen or fresh chickens. each kitchen was visited on four occasions. in the large commercial kitchen environmental contamination was found on each visit, whereas campylobacters were isolated on six of the twelve visits to the other kitchens. the hands of operatives were contaminated with campylobacters on only two of the 45 swabs taken ...19846736643
occurrence of campylobacter jejuni in country chicken in calcutta. 19846746032
systematic investigation of enrichment media for wild-type campylobacter jejuni strains.of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of campylobacter jejuni from cecal contents of chickens and chicken livers. it allowed the retrieval of 1 cfu as did solid media but permitted the screening of 50-times larger volumes. double-strength enrichment medium required 5 to 10 cfu for growth. omission of lauryl sulfate reduced the ...19846746890
epidemiological investigations on campylobacter jejuni in households with a primary infection.fifty-four rotterdam patients in which a primary infection with campylobacter jejuni had been detected (index patients) were compared with 54 control subjects with regard to the consumption and preparation of foods 7 days before onset of illness and the keeping of pet animals. significantly more index patients than controls had eaten chicken meat (47 v. 29; p = 0.0002), particularly at barbecues (14 v. 2; p = 0.0015). marginally more index patients had eaten pork (47 v. 39; p = 0.048) or inadequ ...19846501878
[sensitivity of campylobacter jejuni/coli to 11 antibiotics].the antimicrobial susceptibility of 106 strains of campylobacter jejuni/coli recovered from animals and humans was studied using broth microdilution panel. human and animal strains exhibit similar susceptibility, with the exception of the strains from pigs which are more resistant to erythromycin and clindamycin. among the hippurate negative strains (c. coli), these strains are also recognizable from those recovered from other hosts.19846611534
intestinal carriage of campylobacter jejuni and salmonella by chicken flocks at slaughter.campylobacter jejuni were isolated in large numbers from the majority of birds sampled in colonic swabs from 28 of 60 flocks at slaughter. by contrast only small numbers of birds from 11 of the same 60 flocks yielded salmonella enteritidis serotypes. three c. jejuni isolates from each flock were serotyped on the basis of their heat-stable antigens, using antisera prepared against 16 serotypes common in campylobacter diarrhea in man. the majority (72 of 83) of the chicken isolates could be seroty ...19846383579
experimental gastroenteritis in newly-hatched chicks infected with campylobacter jejuni.the susceptibility of chicks to enteritis caused by campylobacter jejuni was studied. three-day-old chicks did not develop enteritis after oral infection but chicks infected within 12 h of hatching developed gastroenteritis. the incubation period correlated with the inoculum size. initially, infected chicks developed blood- and mucus-containing stools, although watery diarrhoea often occurred late in the course of the disease. recurrences of the enteric manifestations were common but only two ou ...19846492120
selected enrichment broths for recovery of campylobacter jejuni from foods.we attempted to shorten the required time for enrichment broth culture for the isolation of campylobacter jejuni. enrichment broths described by doyle and roman and park and stankiewicz and one developed during this study were compared for ability to isolate c. jejuni from raw chicken carcasses. our medium was a modification of that of doyle and roman with the addition of filter-sterilized fbp (0.2% ferrous sulfate, 0.025% sodium metabisulfite, 0.05% sodium pyruvate), 0.1% sodium lauryl sulfate, ...19846476833
efficacy of media and methods for detecting and enumerating campylobacter jejuni in refrigerated chicken meat.a study was undertaken to compare several enrichment and direct isolation media for their suitability to detect and enumerate five strains of campylobacter jejuni in refrigerated (5 degrees c) chicken meat. the influence of co2 on survival at 5 degrees c was also investigated. selective enrichment media evaluated included preston broth (pb), selective semisolid brucella medium (ssbm), campylobacter enrichment broth (ceb), vtp brucella-fbp broth (vtp), rosef and kapperud campylobacter enrichment ...19854083888
laboratory infection of chicken eggs with campylobacter jejuni by using temperature or pressure differentials.fertile chicken eggs were infected in our laboratory with campylobacter jejuni suspensions by using temperature or pressure differential methods of inoculation. after 2 days of incubation, over 90% of the eggs carried c. jejuni when iron was present in the inoculum. this percentage declined rapidly until by day 8, less than 10% of the eggs were detectably infected. however, up to 11% of hatched, healthy chicks carried c. jejuni in their intestinal tracts. the isolated organisms were of the same ...19854015086
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild animals.by using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam.a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ...19854031520
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...19854044793
[isolation of campylobacter jejuni and campylobacter coli from intestinal contents and feces of chickens]. 19853925041
[occurrence and transmission of campylobacter jejuni/coli in young poultry fattening production. 1]. 19853910389
serotyping of and hippurate hydrolysis by campylobacter jejuni isolates from human patients, poultry and pigs in the netherlands.three hundred strains of campylobacter jejuni, isolated from human patients, poultry and pigs were serotyped according to the penner-lauwers system and furthermore tested for hippurate hydrolysis. serotyping showed a close relationship between human and chicken strains, whereas there was little relationship between human and pig strains. a similar conclusion was drawn from the results of the hippurate hydrolysis test: 86% of human and 94% of poultry strains were positive, whereas 91% of pig stra ...19854004203
transmission of campylobacter jejuni by the housefly (musca domestica).houseflies (musca domestica) were infected with campylobacter jejuni after being confined for 5 days in a horsfall isolator containing 25-day-old chickens known to be fecal excretors of the organism. contaminated flies, when subsequently transferred to a second unit, transmitted c. jejuni to specific-pathogen-free chickens. allowing a sample of 32 houseflies to ingest c. jejuni in a liquid suspension resulted in recovery rates of 20% from the feet and ventral surface of the body and 70% from the ...19854026732
role of litter in the transmission of campylobacter jejuni.autoclaved or non-autoclaved used broiler litter that was experimentally contaminated with campylobacter jejuni was capable of infecting specific-pathogen-free chicks maintained in modified horsfall isolators. artificially infected chicks became fecal shedders of c. jejuni, resulting in contamination of both autoclaved and non-autoclaved used broiler litter. fecal shedding of c. jejuni by litter-reared, artificially infected chicks persisted for at least 63 days after chicks were transferred to ...19854026733
[bacteriological and serological evidence for the presence of campylobacter jejuni in poultry, animals and man (1982-1983)]. 19854070879
serotyping of campylobacter jejuni from an outbreak of enteritis implicating chicken.an outbreak of campylobacter enteritis involving 7 of 17 people over a period of 5 days followed a dinner at a restaurant. a chicken casserole dish was implicated with a food-specific attack rate of 58%. campylobacter jejuni penner serotype 18/21/29, resistant to metronidazole, was isolated from 3 of 4 symptomatic patients and from three raw fresh chicken samples closely associated with the implicated chicken. numbers of c. jejuni in the chicken ranged from 5.3 x 10(1) to 7.5 x 10(2) colony form ...19854056451
[electron microscope observation of campylobacter jejuni]. 19853837358
[isolation and identification of campylobacter jejuni from chicken flocks]. 19853837359
the effect of carbon dioxide packaging on detection of campylobacter jejuni from chicken carcasses.commercially-processed broilers were held at 4 c in carbon dioxide(co2)-flushed bags or in natural atmosphere for nine days prior to determining the most probable number of campylobacter jejuni on individual carcasses. fifty broilers were evaluated. the co2-enriched atmosphere packaging of broilers had no detectable effect on the c. jejuni populations.19853923461
isolation of campylobacter jejuni from market chickens: effect of temperature and time of incubation.during a 4-month period, starting in june 1983, campylobacter jejuni was isolated at 42% from a total of 115 samples of market chickens. the effects of the temperature and duration of incubation on the isolation of c. jejuni were evaluated. the samples were massaged in polyethylene plastic bags, filtered through cheesecloth, centrifuged, and the sediment was plated on a selective medium. isolation of c. jejuni was increased by incubating at 42 degrees c during 48 h as opposed to 35 degrees c for ...19854096141
epidemiologic study of campylobacteriosis in iowa cattle and the possible role of unpasteurized milk as a vehicle of infection.bile samples were collected from 477 iowa dairy cows and were cultured for thermophilic campylobacters. the prevalence of thermophilic campylobacters in the bile was 15.5%. campylobacter jejuni and c coli from dairy cattle, chickens, pigs, sheep, and human beings were serotyped to develop host-species profiles. human and cattle serologic profiles were the most similar, and human and chicken profiles shared several similarities. epidemiologic data from 168 human cases of campylobacteriosis indica ...19863954200
a survey of campylobacter and other bacterial contaminants of pre-market chicken and retail poultry and meats, king county, washington.as part of a larger study to determine the flow of campylobacter and salmonella from food animals to humans during 1982-83, 1,936 swabs were collected for bacteriologic study from pre-market chickens, retail poultry, and other retail meats as well as from equipment and work surfaces used to process such foods. of the 297 samples collected in a poultry processing plant, 56.6 per cent were positive for campylobacter jejuni/coli (cjc), as were 23.1 per cent of the 862 retail chicken, and 17.2 per c ...19863953916
the role of poultry and meats in the etiology of campylobacter jejuni/coli enteritis.to determine the role of meats as possible sources of infection leading to campylobacter jejuni/coli (cjc) enteritis, 218 cases and 526 controls were selected from the king county group health cooperative (ghc) population from april 1982 through september 1983. all subjects were interviewed regarding food consumption one week prior to case onset. consumption of chicken and cornish game hen were both associated with more than a doubling of the risk of cjc enteritis: for chicken (relative risk = 2 ...19863953917
natural campylobacter colonization in chickens raised under different environmental conditions.a cross-sectional study of 447 laying hens (age range 0-65 weeks) and a longitudinal study of 164 similar birds showed that campylobacter jejuni was not present in the faeces of newly hatched chicks, but that colonization arose after 5-9 weeks. a survey of 250 broilers obtained from four breeders showed that all were negative for c. jejuni before and after slaughter at the age of 5 weeks. once c. jejuni had appeared in a flock, it rapidly spread to virtually all birds, but at the age of 42 weeks ...19863734424
the viability of campylobacter jejuni on refrigerated chicken drumsticks.radappertized chicken drumsticks were experimentally contaminated with suspensions of campylobacter jejuni in two trials. qualitative analysis on drumsticks with an initial level of contamination of 4.8 x 10(3) cfu/cm2 showed that viability was retained for at least 10 days of storage at either 9 degrees or -12 degrees c. in a second quantitative trial, the level of contamination declined from 9.9 x 10(2) cfu/cm2 to 4.5 x 10(1) cfu/cm2 after 7 days at -20 degrees c. thereafter, c. jejuni persist ...19863798737
campylobacter jejuni contamination of eggs.contamination of commercial table eggs with a fecal suspension containing 4.4 x 10(6) cfu/g campylobacter jejuni resulted in shell penetration in 3/70 eggs and recovery of the organism from homogenized egg contents in 1/70 eggs. viability of c. jejuni on the shell surface was retained for only 16 hours, attributed to desiccation of the fecal suspension. a field survey of three commercial laying farms and their associated egg-packing plants showed that hens demonstrated to be fecal shedders of c. ...19863798738
isolation of campylobacter jejuni from intestinal contents of chickens in mexico city. 19863738283
campylobacter colitis in ranch mink in ontario.outbreaks of colitis, where campylobacter jejuni and campylobacter coli were the only pathogens isolated occurred in weanling mink (mustella vision) on two commercial mink ranches in ontario. lesions were restricted to the proximal colon and were characterized by multiple 1 mm focal or 1 mm linear erosions/ulcers in the region 2 cm distal to the ileal-colonic junction. histological changes included thickening of the colonic mucosa, inflammatory cell infiltrate in the lamina propria and submucosa ...19863742356
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in campylobacter jejuni.three human isolates of campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. increased numbers of longer, more filamentous forms were seen in gram-stained smears. three proteins, with apparent mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. at lea ...19862944843
biochemical and serological characteristics of campylobacter jejuni strains isolated from chickens. 19862953191
raw beef, pork and chicken in japan contaminated with salmonella sp., campylobacter sp., yersinia enterocolitica, and clostridium perfringens--a comparative study.one hundred and twenty samples each of raw ground beef, pork and chicken from ten local grocery stores in shimane prefecture, japan, were examined for the presence of salmonella sp. (sal), campylobacter jejuni (cj), campylobacter coli (cc), yersinia enterocolitica (ye), and clostridium perfringens (cp) from april 1984 to march 1985. a total of 205 isolates of sal (112 strains), cj (64 strains), cc (one strain), ye (7 strains) and cp (21 strains) were recovered from 17 beef (14.2%), 31 pork (25.8 ...19872887078
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
[campylobacter jejuni in poultry processed in slaughterhouses].the frequency of occurrence of campylobacter jejuni germs in dressed poultry was studied for a year. the samples--smears from the body cavities of chickens--were collected during the technological dressing of the chickens; 101 strains of campylobacter jejuni (i. e. 28.69%) were isolated from the 352 samples analyzed. the occurrence of the germs exhibited a considerable seasonal variance with peak rates in spring and summer. the use of a suitable culture medium, the technique of cultivation and t ...19873118554
survival of thermophilic campylobacters on fingertips and their elimination by washing and disinfection.a simple impression-plate technique has been used to investigate the survival of four thermophilic campylobacter strains applied to fingertips. campylobacters suspended in 0.1% peptone water and dried on the fingertips survived for different periods of time ranging from less than 1 to greater than or equal to 4 min. however, campylobacters suspended in chicken liquor or blood survived for much longer periods. the most resilient organism was campylobacter jejuni nctc 11392 which, when suspended i ...19873678391
serum agglutinin titers against somatic and flagellar antigens of campylobacter jejuni and isolation of campylobacter spp in chickens.from june 1983 to june 1984, two hundred twenty-five 3- to 30-month-old chickens (hens) on 10 different farms were examined for campylobacter spp. watering trays and fly vectors also were examined for campylobacter spp on 6 of the 10 farms. campylobacter jejuni was isolated from fecal specimens from 64 hens (28.4%), c coli was isolated from 6 hens (2.7%), and c laridis was isolated from 9 hens (4%). the isolation rate of c jejuni was 6.7% to 46.7% for 9 of the 10 farms. on 2 farms, agglutinin ti ...19873592379
decontamination of campylobacter jejuni on chicken drumsticks using chemicals and radiation.a trial was conducted to compare the efficacy of four disinfectants and radiation to reduce the level of c. jejuni contamination on poultry meat. two levels of each treatment were applied to chicken drumsticks inoculated with a known concentration of a strain of campylobacter jejuni, biotype 1, isolated from a human patient with diarrhea. radiation using a cobalt-60 source at a level of 0.5 kgy effected a 99% surface reduction in c. jejuni. with a mean initial surface contamination level of 1.1 ...19873576968
serogroups of thermophilic campylobacters from humans and from non-human sources, israel 1982-1985.the distribution of serogroups of thermophilic campylobacters isolated in israel from human patients (2421 isolates), chicken (942), turkeys (158), cattle (398), wild birds (234) and other sources, was studied. among the human isolates, 74 rog-serogroups were identified. the six most commonly isolated of these (1, 18; 11; 12; 8,23; 4 and 5,39) were found frequently in chickens. only four common serogroups in man were also common in cattle, three in turkeys and two in wild birds. two common serog ...19873678392
prevalence of campylobacter jejuni in eggs and poultry meat in new york state.the presence of campylobacter jejuni was tested for but not isolated from any of 276 eggs sampled from 23 egg farms in new york state. the presence of c. jejuni was evaluated in broilers, kosher broilers, spent layers, peking ducks, and turkeys. four of five poultry dressing plants tested showed positive growth of c. jejuni on the 25-carcass samples at various stages of processing. twenty to 100% of live birds sampled contained c. jejuni on the skin but 90 to 100% were contaminated after scaldin ...19873447135
[experimental animal studies with campylobacter jejuni and campylobacter coli in chickens]. 19873566434
efficacy of some methods and media for detecting and enumerating campylobacter jejuni in frozen chicken meat.four of five strains of campylobacter jejuni survived in chicken meat stored at -18 degrees c for 12 months. direct plating of samples was superior to the most probable number technique for enumerating c. jejuni. enrichment culture using the doyle & roman enrichment method resulted in the highest rates of detection. packaging under an atmosphere of co2 did not substantially influence survival.19873597202
campylobacter enteritis at a university: transmission from eating chicken and from cats.campylobacter jejuni is the most common enteric pathogen isolated from university and college students in the united states. during the fall and winter quarters of the 1983-1984 academic year, the authors conducted a case-control study at the university of georgia, athens, georgia, to identify risk factors for c. jejuni enteritis. students with diarrhea whose cultures yielded c. jejuni were compared with controls matched by age, sex, and residence. a total of 45 case-control pairs were interview ...19873618583
enterotoxigenicity of chicken isolates of campylobacter jejuni in ligated ileal loops of rats.ligated ileal loops in rats of the charles-foster strain, weighing 200-250 g and aged 7-8 months, provided a sensitive and reproducible means of testing the enterotoxigenicity of campylobacter jejuni. all of 16 chicken isolates caused fluid accumulation comparable to that produced by toxigenic vibrio cholerae o1, strain 569b. however, 11 of the isolates required one to three consecutive passages through the rat gut before doing so. the amount of fluid produced increased after each passage. of th ...19883385766
colonization characteristics of campylobacter jejuni in chick ceca.we report our findings on several parameters influencing cecal colonization of chickens by campylobacter jejuni. thirty-five colony-forming units (cfu) of a composite culture of c. jejuni colonized the ceca of one-half of the newly hatched chicks challenged by oral gavage. a challenge dose of 3500 cfu/chick consistently colonized the ceca of all chicks challenged. challenge doses of approximately 10(5) cfu of c. jejuni per chick resulted in consistent cecal colonization, regardless of whether th ...19883401176
paediatric campylobacter diarrhoea from household exposure to live chickens in lima, peru.although campylobacter jejuni is a frequent enteropathogen in cases of paediatric diarrhoea in developing countries, its route of transmission is not well understood. an age-matched, case-control study of children with c. jejuni diarrhoea was therefore carried out in lima, peru, from january 1983 to april 1986 to identify the risk factors and vehicles of transmissions. as cases, 104 children less than 3 years of age were selected and compared with controls of the same age with non-gastrointestin ...19883262442
the extent of surface contamination of retailed chickens with campylobacter jejuni serogroups.eighty-two chickens purchased at 11 retailers (supplied by 12 wholesalers) in the south of england were cultured for campylobacter jejuni by a method involving total immersion. the organism was isolated from 22 (48%) of 46 fresh birds, 12 of 12 uneviscerated (new york dressed) birds, but only 1 of 24 frozen birds. viable counts of up to 1.5 x 10(6)/chicken were obtained from fresh birds and 2.4 x 10(7)/chicken from uneviscerated birds. surface swabbing of breasts, thighs, wings and vents of fres ...19883338503
chemotactic behavior of campylobacter jejuni.the chemotactic behavior of campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. l-fucose was the only carbohydrate and l-aspartate, l-cysteine, l-glutamate, and l-serine were the only amino acids producing a chemotactic (positive) response. several salts of organic acids, including pyruvate, succinate, fumarate, citrate, malate, and alpha-ketoglutarate, were also chemoattractants, as were ...19883372020
incidence of campylobacter jejuni in processed egg products.egg samples were obtained from two commercial egg-processing facilities to determine if campylobacter jejuni could be isolated from the raw product or from further-processed egg products intended for human consumption. no c. jejuni were detected in any of the egg or egg-product samples or from water samples collected from the overflow of the egg washer. campylobacter jejuni was recovered from all control samples, which had been inoculated at the plants. these findings suggest that properly proce ...19883194336
the growth of potential food poisoning organisms on chicken and pork muscle surfaces.muscle surfaces of pork were inoculated with a mixture of yersinia enterocolitica and staphylococcus aureus, and chicken muscle with campylobacter jejuni or a mixture of salmonella typhimurium and staph. aureus. the surface growth at 20 degrees c was followed microscopically. organisms grew as discrete colonies bound together by a glycocalyx which differed between bacterial species. on prolonged incubation colonies spread peripherally and tended to coalesce, while still retaining their colony st ...19883072327
serovars and biovars of campylobacter strains isolated from humans and slaughterhouse animals in northern germany.a total of 318 campylobacter strains from sporadic cases of human enteritis (109 strains) and healthy slaughterhouse animals in northern germany (209 strains) were bio- and serotyped according to the lior typing schemes. three hundred strains were typable (94.3%) and 38 serovars were identified. among human strains 28 serovars were identified with 30% of them belonging to serovar 4. strains from pigs were associated with 25 serovars, the most frequent being serovar 20 (21.2%). fourteen serovars ...19883372398
intestinal colonization of campylobacter jejuni in chickens. 19883386104
[survey for the contamination of campylobacter jejuni and campylobacter coli among raw meat and the environment of poultry processing plants]. 19883137280
[epidemiological aspects of enteritis due to campylobacter jejuni].from september 1986 through july 1987, all fecal specimens obtained from infants and children who visited the pediatric clinic of the shizuoka general hospital with complaints of abdominal pain or diarrhea were examined for thermophilic campylobacters. bacteriological and epidemiological studies were performed on household contacts. the mothers of the patients were compared with the mothers of age-matched control subjects. bacteriological examination of animals in 49 primary schools in shizuoka ...19892501423
serotyping studies of campylobacter from naturally colonized chickens.campylobacter jejuni/coli strains from 164 chickens were serotyped by the methods previously described by penner et al. and lior et al. the chickens were sampled during breeding from hatching to the age of 42 weeks. the birds were housed, in two separate groups, under different environmental conditions, (for comparison of the effect of hygienic precautions on the transmission of the bacteria during breeding). in the group where the hygienic conditions could be controlled to a greater extent, the ...19892703018
[campylobacter infection and campylobacter excretion in turkeys].five turkey poults at an age of 27 days were infected with campylobacter jejuni strain. additional five poults were infected by contact. turkeys infected by contact and artificially were necropsied 1 and 3 months later, respectively. the histological examination of the liver revealed degenerative changes in contact birds and a proliferation of the connective tissue and bile-ducts in two of the oral infected poults. campylobacter spp. were excreted daily during the first two weeks and afterwards ...19892590155
better growth of campylobacter jejuni using simple fortner's principle & candle extinction jar.c. jejuni isolated from patients of childhood diarrhoea and chicken intestines were examined for better growth in both liquid and solid media under three different environments of candle extinction jar with or without an escherichia coli inoculated plate and three such plates in a sealed jar. better growth by one log in liquid media was obtained at 24 and 48 h with bacterial counts of about ca. 10(7) and ca. 10(9) respectively, and larger colony size at 24 h on solid medium by inclusion of a sin ...19892644169
campylobacter fetus subsp. jejuni in poultry reared under different management systems in nigeria.cloacal swabs from 487 live birds in 36 flocks and 70 poultry carcasses were cultured for campylobacter fetus subsp. jejuni. it was isolated from 12.3% of the birds in 19 flocks. chickens, turkeys, and guinea fowl differed from one another in isolation rates of the organism. management system affected its occurrence, and only 7.1% of eviscerated carcasses yielded it. it was concluded that bird species, management system, and immersing slaughtered poultry in boiling water before dressing affect r ...19892695052
virulence of campylobacter jejuni for chicken embryos.the pathogenicity of campylobacter jejuni was examined in chicken embryos. in this system, mortality data and histopathological findings induced by organisms and by bacterium-free filtered broth were identical. the absence in chicken embryo tissues both of organisms and of an inflammatory infiltrate suggests a toxin etiology.19892754005
inhibition of campylobacter jejuni in vitro by broiler chicken caecal contents. 19892800258
[the pathogenicity of campylobacter jejuni as a monoinfection and as a mixed infection with escherichia coli 078:k80 in broilers].two week old broilers (n = 61) with a monoinfection with campylobacter jejuni (0.5 ml of suspension containing 10(5) cfu/ml per os) showed reduced increase in weight during week 3 after infection compared to the control group. an other group of chickens (n = 31) was additionally infected with a suspension of escherichia (e.) coli o78:k80 via drinking water from day 4 to 6 after the primary infection. this mixed infection provoked clinical signs of a disease and reduced increase in weight during ...19902241888
fecal contamination of shanty town toddlers in households with non-corralled poultry, lima, peru.we used direct observer techniques to measure the frequency with which toddler-aged children were contaminated by poultry feces in homes in a peri-urban shanty town in lima, peru. the mean number of fowl was 5.4 (sd 3.1), with 10.0 (sd 10.7) poultry defecations per 12 hours. toddlers' hand contact with poultry feces occurred a mean of 2.9 (sd 3.0) times/12 hours. a mean of 3.9 (sd 4.6) feces-to-mouth episodes per household/12 hours occurred both by direct hand-to-mouth contamination and indirect ...19902297055
production and characterisation of campylobacter jejuni enterotoxin in a synthetic medium and its assay in rat ileal loops.a synthetic medium for production of campylobacter jejuni enterotoxin was developed for the purposes of its purification by modifying syncase medium, replacing sucrose with glucose, and supplementing with 0.025% sodium pyruvate, 0.25% sodium metabisulphite, 0.001% ferric chloride and 0.1% l-cysteine, adjusted to ph 6.7. culture filtrates of a human diarrhoeal and a chicken isolate, grown in this medium caused fluid accumulation ranging between 0.50-0.70 ml/cm of rat ileal loop. the kinetics of t ...19902323551
a further study of the epidemiology of campylobacter jejuni infections.epidemiological data on notifications of campylobacter jejuni infections is given for the christchurch health district area. the number of notifications rose steadily to a peak of 883 cases in 1987 (rate 261 per 100,000 population). christchurch shows consistently higher rates than the rest of new zealand. peak incidence is in the 0-4 age group; there is a high incidence amongst males and in rural areas; peak incidences in spring/summer; occupational risks have not been identified; chicken consu ...19902342690
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