Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| experimentally confirmed toltrazuril resistance in a field isolate of cystoisospora suis. | constant treatment regimens with toltrazuril against cystoisospora suis infection in piglets are being applied in the intensive production systems for the last two decades, but the possibility of resistance development has not been addressed so far despite limited availability of treatment alternatives. recently, a pig producer in the netherlands who routinely used toltrazuril complained about diarrhea in suckling piglets in the absence of bacterial and viral pathogens, and oocysts of c. suis co ... | 2017 | 28662668 |
| cloning, expression and molecular characterization of a cystoisospora suis specific uncharacterized merozoite protein. | the genome of the apicomplexan parasite cystoisospora suis (syn. isospora suis) has recently been sequenced and annotated, opening the possibility for the identification of novel therapeutic targets against cystoisosporosis. it was previously proposed that a 42 kda uncharacterized merozoite protein, encoded by gene csui_005805, might be a relevant vaccine candidate due to its high immunogenic score, high expression level and species-specificity as determined in silico. | 2017 | 28173829 |
| the genome of the protozoan parasite cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates. | vaccine development targeting protozoan parasites remains challenging, partly due to the complex interactions between these eukaryotes and the host immune system. reverse vaccinology is a promising approach for direct screening of genome sequence assemblies for new vaccine candidate proteins. here, we applied this paradigm to cystoisospora suis, an apicomplexan parasite that causes enteritis and diarrhea in suckling piglets and economic losses in pig production worldwide. using next generation s ... | 2017 | 28161402 |
| occurrence, clinical involvement and zoonotic potential of endoparasites infecting swiss pigs. | in order to estimate the diversity, clinical involvement and zoonotic potential of parasites in pigs submitted for diagnosis to the pathopig project of the swiss federal food safety and veterinary office, faeces (n=125) from suckling piglets (n=39), weaners (n=60) and piglets beginning fattening (n=26) from 74 swiss farms were examined by 3 coproscopical methods (i.e. sedimentation/zinc chloride-flotation; safc and ziehl-neelsen staining). samples microscopically positive for cryptosporidium wer ... | 2016 | 27639842 |
| first report of molecular identification of cystoisospora suis in piglets with lethal diarrhea in japan. | cystoisospora suis is a pathogen that causes diarrhea in pigs and can lead to serious disease. species identification, especially by histopathological examination, is often difficult because of morphologically similar parasites such as eimeria species. in this study, we used histopathological, bacteriological, virological, and parasitological methods to identify the cause of the disease in two piglets with severe diarrhea. villous atrophy, diffuse necrosis, and flattening of mucosal epithelial c ... | 2016 | 27078667 |
| exploration of fluorescence-based real-time loop-mediated isothermal amplification (lamp) assay for detection of isospora suis oocysts. | isospora suis is an intestinal protozoan parasite in pigs. the 2-3 weeks old piglets are most often infected by i. suis because their immune system is not fully developed. the infection exhibits clinical features such as diarrhea and dehydration and seriously affects the economic interests of farmers. the traditional method of identifying i. suis relies on the detection of fecal oocysts, which depends heavily on the accumulation of experience. thus, missed detection, and false alarms often occur ... | 2016 | 26965400 |
| case-control study of pathogens involved in piglet diarrhea. | diarrhea in piglets directly affects commercial swine production. the disease results from the interaction of pathogens with the host immune system and is also affected by management procedures. several pathogenic agents such as campylobacter spp., clostridium perfringens, escherichia coli, salmonella spp., group a rotavirus (rv-a), coronaviruses (transmissible gastroenteritis virus; porcine epidemic diarrhea virus), as well as nematode and protozoan parasites, can be associated with disease cas ... | 2016 | 26754836 |
| cystoisospora suis - a model of mammalian cystoisosporosis. | cystoisospora suis is a coccidian species that typically affects suckling piglets. infections occur by oral uptake of oocysts and are characterized by non-hemorrhagic transient diarrhea, resulting in poor weight gain. apparently, primary immune responses to c. suis cannot readily be mounted by neonates, which contributes to the establishment and rapid development of the parasite, while in older pigs age-resistance prevents disease development. however, the presence of extraintestinal stages, alt ... | 2015 | 26664994 |
| field evaluation of the effectiveness of an oral toltrazuril and iron combination (baycox® iron) in maintaining weaning weight by preventing coccidiosis and anaemia in neonatal piglets. | effectiveness of an oral combination of toltrazuril and iron dextran (baycox(®) iron) to maintain weaning weight by preventing coccidiosis caused by isospora suis and iron-deficiency anaemia in neonatal piglets was investigated on three commercial pig farms with a history of coccidiosis: two in mexico and one in brazil. on day (sd) 2 of life, piglets were randomised within litter by bodyweight to treatment or control group. on sd 3 piglets allocated to the control group (cg) each received 1 ml b ... | 2015 | 26152420 |
| superinfection of sows with cystoisospora suis ante partum leads to a milder course of cystoisosporosis in suckling piglets. | cystoisospora (syn. isospora) suis is a leading cause of diarrheal disease in neonatal piglets. to address the possibility of maternal immunization against c. suis infection six non-naïve pregnant sows were superinfected with 100,000 oocysts 2 weeks ante partum and compared to non-superinfected animals. their piglets were infected with 1000 oocysts on the third day of life. clinical and parasitological parameters as well as antibody titers in colostrum/milk and blood of sows and in the blood of ... | 2014 | 24877784 |
| the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain and mortality in 4 danish pig herds. | the study evaluated the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain (adg) and mortality and described the clinical manifestations in four herds suffering from the syndrome. nnpds is a diarrhoeic syndrome affecting piglets within the first week of life, which is not caused by enterotoxigenic escherichia coli (etec), clostridium perfringens (c. perfringens) type a/c, clostridium difficile (c. difficile), rotavirus a, coronavirus, cystoisospora suis, strongyloide ... | 2014 | 24755093 |
| the prevalence and diversity of intestinal parasitic infections in humans and domestic animals in a rural cambodian village. | in cambodia, intestinal parasitic infections are prevalent in humans and particularly in children. yet, information on potentially zoonotic parasites in animal reservoir hosts is lacking. in may 2012, faecal samples from 218 humans, 94 dogs and 76 pigs were collected from 67 households in dong village, preah vihear province, cambodia. faecal samples were examined microscopically using sodium nitrate and zinc sulphate flotation methods, the baermann method, koga agar plate culture, formalin-ether ... | 2014 | 24704609 |
| immune response to cystoisospora suis in piglets: local and systemic changes in t-cell subsets and selected mrna transcripts in the small intestine. | infections of neonatal piglets with cystoisospora suis are responsible for substantial economic losses in pig production. to investigate kinetics of t-cell populations, which are possibly involved in this infection, lymphocytes from blood, spleen, mesenteric lymph nodes and the jejunal mucosa of infected and noninfected piglets were investigated by flow cytometry and immunohistochemistry at five time points during the acute phase of primary infection. additionally, mrna expression levels of patt ... | 2014 | 24702033 |
| which factors influence the outcome of experimental infection with cystoisospora suis? | for reliable predictions of clinical and parasitological outcome of experimental infections with parasites, different models must be evaluated for possible influences of infection time point, infection dose and host-specific parameters such as breed or litter size. to address these issues for cystoisospora (syn. isospora) suis, the causative agent of porcine neonatal coccidiosis, 181 piglets from 90 litters (hybrid crosses of different breeds) were included in a retrospective study to evaluate d ... | 2014 | 24643624 |
| fluorescence in situ hybridization investigation of potentially pathogenic bacteria involved in neonatal porcine diarrhea. | neonatal diarrhea is a multifactorial condition commonly present on pig farms and leads to economic losses due to increased morbidity and mortality of piglets. immature immune system and lack of fully established microbiota at birth predispose neonatal piglets to infection with enteric pathogens. the microorganisms that for decades have been associated with enteritis and diarrhea in suckling piglets are: rotavirus a, coronavirus, enterotoxigenic escherichia coli (etec), clostridium perfringens t ... | 2014 | 24628856 |
| a retrospective study on the etiological diagnoses of diarrhea in neonatal piglets in ontario, canada, between 2001 and 2010. | laboratory surveillance data from the animal health laboratory, university of guelph, on the etiological diagnoses of neonatal diarrhea in piglets were analyzed to determine the relative importance and trends of different enteric pathogens in ontario. a total of 237 cases, including live and dead 1- to 7-day-old piglets, were submitted for diagnosis of gastrointestinal illness between 2001 and 2010. the combined frequencies for cases of gastrointestinal illness involving escherichia coli, clostr ... | 2013 | 24124267 |
| microbiological, pathological and histological findings in four danish pig herds affected by a new neonatal diarrhoea syndrome. | neonatal diarrhoea is a frequent clinical condition in commercial swine herds, previously regarded to be uncomplicated to treat. however, since 2008 it seems that a new neonatal diarrhoeic syndrome unresponsive to antibiotics and common management practices has emerged. routine laboratory examinations have not detected any pathogen related to this syndrome. the primary purpose of this study was to evaluate if well-known enteric pathogens could be associated with outbreaks of neonatal diarrhoea, ... | 2013 | 24119974 |
| transfer of cystoisospora suis-specific colostral antibodies and their correlation with the course of neonatal porcine cystoisosporosis. | cystoisospora suis is the most pathogenic species of coccidia in suckling piglets, affecting them predominantly within their first three weeks of life. the clinical signs of neonatal cystoisosporosis include watery diarrhea and wasting, leading to significant economic losses for the farmer. since neonatal piglets have an immature immune system, colostral transfer of maternal factors such as immune cells or antibodies is essential for controlling infections at that age. however, the role of c. su ... | 2013 | 23932639 |
| isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia. | coccidian parasites are of major importance in animal production, public health and food safety. the most frequently used representative in basic research on this group is toxoplasma gondii. although this parasite is well investigated there is no adequate in vitro model for its sexual development available and knowledge on this important life cycle phase is therefore scarce. the use of isosporasuis, a sister taxon to t. gondii and the causative agent of piglet coccidiosis, could provide a soluti ... | 2013 | 23861983 |
| factors associated with the occurrence and level of isospora suis oocyst excretion in nursing piglets of greek farrow-to-finish herds. | piglet isosporosis is one of the most common parasitic diseases in modern pig production. to prevent clinical disease, prophylactic treatment of piglets with toltrazuril (baycox® 5%, bayer healthcare, animal health, monheim, germany) is widely practiced in the past 20 years. there are only very few reports documenting the likely effect of managerial practices, such as hygiene measures, all-in-all-out management of farrowing facilities and piglet manipulations, and/or farm-specific environment - ... | 2012 | 23174007 |
| recovery of gastrointestinal swine parasites in anaerobic biodigester systems. | solid and liquid wastes from livestock operations represent important challenges for animal production regarding their impact in the environment and public health. parasitological tests performed on 80 samples of affluent and effluent waters from three anaerobic biodigestors with flexible structure from swine farms of caldas - colombia, showed the presence of isospora suis (45%), eimeria suis (42.5%), e. espinosa (35%), strongyloides ransomi (28.8%), e. perminuta (12.5%), e. cerdonis (3.8%), and ... | 2012 | 23070435 |
| several enteropathogens are circulating in suckling and newly weaned piglets suffering from diarrhea in the province of villa clara, cuba. | intestinal contents of suckling (n = 45) and newly weaned (n = 45) piglets, suffering from diarrhea in the province of villa clara in cuba, were tested for viral, bacterial, and parasitic enteropathogens from may to june 2008. at least one enteropathogen was identified in 53.3 % of piglets and enterotoxigenic escherichia coli (etec; 25.6 %) was the major pathogen; mostly sta(+)/stb(+) or f4(+)/sta(+)/stb(+) etec were isolated. the overall occurrence of the rest of pathogens was 10 % for transmis ... | 2013 | 22843242 |
| cystoisospora spp. from dogs in china and phylogenetic analysis of its 18s and its1 gene. | cystoisospora spp. oocysts isolated from dog feces in changchun, china were morphologically similar to those of cystoisospora ohioensis and cystoisospora sp. 1-mm recently isolated from dogs in japanese. sequencing results of the 18s subunit rna gene from isolates in the present study were compared to other cystoisospora spp. and the results suggested that cystoisospora spp. from dogs in changchun was homologous to c. ohioensis and cystoisospora sp. 1-mm. phylogenetic analysis of the 18s rrna se ... | 2012 | 22726385 |
| prevalence of coccidian infection in suckling piglets in china. | to determine the prevalence of coccidian infection in suckling piglets in china, fecal samples from 779 litters of suckling piglets were collected on 80 different farms in 17 provinces from september 2009 to december 2010. these samples were examined through saturated saline flotation technique. the prevalences of coccidian infection ranged from 0 to 32.5% among different provinces and the average was 16.7% (130/779). the highest prevalence of 19.9% (69/346) was found in 8-14 day-old litters of ... | 2012 | 22694832 |
| Necrotic enteritis due to simultaneous infection with Isospora suis and clostridia in newborn piglets and its prevention by early treatment with toltrazuril. | In this study, 51 piglets originating from five different sows were included in the investigations. The animal source of all sows had a history of Clostridium perfringens type A (ß2) infection. The piglets of three sows (n?=?31) were experimentally infected with Isospora suis within the first 4 h after birth and were randomly assigned to the treatment group or the sham-dosing group. The piglets of the two remaining sows (n?=?20) served as I. suis-uninfected controls. Twelve hours post-infection, ... | 2011 | 21968954 |
| Necrotic enteritis due to simultaneous infection with Isospora suis and clostridia in newborn piglets and its prevention by early treatment with toltrazuril. | In this study, 51 piglets originating from five different sows were included in the investigations. The animal source of all sows had a history of Clostridium perfringens type A (ß2) infection. The piglets of three sows (n?=?31) were experimentally infected with Isospora suis within the first 4 h after birth and were randomly assigned to the treatment group or the sham-dosing group. The piglets of the two remaining sows (n?=?20) served as I. suis-uninfected controls. Twelve hours post-infection, ... | 2011 | 21968954 |
| Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment. | Quantification of immunohistochemical results constitutes an important tool in the analysis of cells and tissue that is not readily replaced by other techniques. For reliable quantification, it is essential to consider factors such as tissue fixation and tissue sampling. We report a study on the model of the intestine of Isospora suis-infected piglets, in which we addressed (1) whether the quantity of detectable T cells in the intestinal mucosa is the same in formalin-, HOPE®-, and cryo-conserve ... | 2011 | 21898671 |
| Immunohistochemical detection and quantification of T cells in the small intestine of Isospora suis-infected piglets-influence of fixation technique and intestinal segment. | Quantification of immunohistochemical results constitutes an important tool in the analysis of cells and tissue that is not readily replaced by other techniques. For reliable quantification, it is essential to consider factors such as tissue fixation and tissue sampling. We report a study on the model of the intestine of Isospora suis-infected piglets, in which we addressed (1) whether the quantity of detectable T cells in the intestinal mucosa is the same in formalin-, HOPE®-, and cryo-conserve ... | 2011 | 21898671 |
| influence of toltrazuril treatment on parasitological parameters and health performance of piglets in the field - an austrian experience. | porcine coccidiosis caused by isospora suis is one of the leading causes of neonatal diarrhea in suckling piglets. currently the only registered drug for metaphylaxis is toltrazuril. to evaluate the effect of treatment on piglets from 7 austrian farms without and 8 austrian farms with toltrazuril application we examined oocyst excretion (including determination of oocysts per gram of feces; opg), diarrhea (fecal score fs 1-4 with 3 and 4 being diarrhea), and general health (health score hs 1-4 w ... | 2011 | 21820246 |
| influence of toltrazuril treatment on parasitological parameters and health performance of piglets in the field - an austrian experience. | porcine coccidiosis caused by isospora suis is one of the leading causes of neonatal diarrhea in suckling piglets. currently the only registered drug for metaphylaxis is toltrazuril. to evaluate the effect of treatment on piglets from 7 austrian farms without and 8 austrian farms with toltrazuril application we examined oocyst excretion (including determination of oocysts per gram of feces; opg), diarrhea (fecal score fs 1-4 with 3 and 4 being diarrhea), and general health (health score hs 1-4 w ... | 2011 | 21820246 |
| efficacy of sulfonamides and baycox(®) against isospora suis in experimental infections of suckling piglets. | sulfonamide treatment of piglets against neonatal coccidiosis has frequently been suggested in the literature. in order to evaluate the efficacy of sulfonamides against experimental isospora suis infections in suckling piglets (oral infection with 1,500 sporulated oocysts of i. suis per piglet on the fourth day of life), two trials were conducted. in trial i, oral sulfadimidine (group sulfa-oral) was applied in doses of 100 mg/kg of body weight (bw) 1 day before infection and 75 mg/kg bw daily f ... | 2011 | 21556685 |
| efficacy of sulfonamides and baycox(®) against isospora suis in experimental infections of suckling piglets. | sulfonamide treatment of piglets against neonatal coccidiosis has frequently been suggested in the literature. in order to evaluate the efficacy of sulfonamides against experimental isospora suis infections in suckling piglets (oral infection with 1,500 sporulated oocysts of i. suis per piglet on the fourth day of life), two trials were conducted. in trial i, oral sulfadimidine (group sulfa-oral) was applied in doses of 100 mg/kg of body weight (bw) 1 day before infection and 75 mg/kg bw daily f ... | 2011 | 21556685 |
| an observational study on the prevalence and impact of isospora suis in suckling piglets in southwestern ontario, and risk factors for shedding oocysts. | an observational study was conducted to determine the prevalence of isospora suis oocysts in fecal samples from suckling piglets in ontario, and to evaluate the relationship between the presence of i. suis oocysts and diarrhea. fifty farms and 709 litters of piglets were included in the study. oocysts were detected on 70% of farms, with 187 litters infected. a litter of pigs that was positive for oocysts was significantly more likely to exhibit diarrhea than a litter that was negative [odds rati ... | 2011 | 21532828 |
| an observational study on the prevalence and impact of isospora suis in suckling piglets in southwestern ontario, and risk factors for shedding oocysts. | an observational study was conducted to determine the prevalence of isospora suis oocysts in fecal samples from suckling piglets in ontario, and to evaluate the relationship between the presence of i. suis oocysts and diarrhea. fifty farms and 709 litters of piglets were included in the study. oocysts were detected on 70% of farms, with 187 litters infected. a litter of pigs that was positive for oocysts was significantly more likely to exhibit diarrhea than a litter that was negative [odds rati ... | 2011 | 21532828 |
| detection and differentiation of coccidian oocysts by real-time pcr and melting curve analysis. | abstract rapid and reliable detection and identification of coccidian oocysts are essential for animal health and food-borne disease outbreak investigations. traditional microscopy and morphological techniques can identify large and unique oocysts, but are often subjective and require parasitological expertise. the objective of this study was to develop a real-time quantitative pcr (qpcr) assay with melting curve analysis (mca) to detect, differentiate, and identify dna from coccidian species of ... | 2011 | 21506835 |
| detection and differentiation of coccidian oocysts by real-time pcr and melting curve analysis. | abstract rapid and reliable detection and identification of coccidian oocysts are essential for animal health and food-borne disease outbreak investigations. traditional microscopy and morphological techniques can identify large and unique oocysts, but are often subjective and require parasitological expertise. the objective of this study was to develop a real-time quantitative pcr (qpcr) assay with melting curve analysis (mca) to detect, differentiate, and identify dna from coccidian species of ... | 2011 | 21506835 |
| faeces, facs, and functional assays--preparation of isospora suis oocyst antigen and representative controls for immunoassays. | summary: highly purified antigen and appropriate controls are essential for antigen-specific immunoassays. in the case of isospora suis, the causative agent of neonatal porcine coccidiosis, the only current source of antigen is oocysts isolated from faeces. the aim of this study was to develop a procedure for high-grade purification of i. suis oocysts from piglet faeces to obtain both antigen and representative controls suitable for in vitro re-stimulation of lymphocytes. this was achieved by us ... | 2010 | 20500923 |
| faeces, facs, and functional assays--preparation of isospora suis oocyst antigen and representative controls for immunoassays. | summary: highly purified antigen and appropriate controls are essential for antigen-specific immunoassays. in the case of isospora suis, the causative agent of neonatal porcine coccidiosis, the only current source of antigen is oocysts isolated from faeces. the aim of this study was to develop a procedure for high-grade purification of i. suis oocysts from piglet faeces to obtain both antigen and representative controls suitable for in vitro re-stimulation of lymphocytes. this was achieved by us ... | 2010 | 20500923 |
| effect of toltrazuril treatment in nursing piglets naturally infected with isospora suis. | isospora suis is an important parasitic infection in intensive pig production worldwide, responsible for significant economic losses. in this study the efficacy of toltrazuril treatment against isosporosis was evaluated, under field conditions and throughout the nursing period, in reducing (i) the mean time to onset of diarrhoea and oocyst excretion, (ii) the odds of diarrhoea and, (iii) the odds and level of oocyst excretion, adjusting for the heterogeneity of i. suis infection among litters an ... | 2010 | 20471754 |
| effect of toltrazuril treatment in nursing piglets naturally infected with isospora suis. | isospora suis is an important parasitic infection in intensive pig production worldwide, responsible for significant economic losses. in this study the efficacy of toltrazuril treatment against isosporosis was evaluated, under field conditions and throughout the nursing period, in reducing (i) the mean time to onset of diarrhoea and oocyst excretion, (ii) the odds of diarrhoea and, (iii) the odds and level of oocyst excretion, adjusting for the heterogeneity of i. suis infection among litters an ... | 2010 | 20471754 |
| changes in lymphocyte populations in suckling piglets during primary infections with isospora suis. | isospora suis, a common intestinal parasite of piglets, causes neonatal porcine coccidiosis, which results in reduced and uneven weaning weights and economic losses in pig production. nevertheless, there are no detailed studies available on the immune response to i. suis. the aim of this study was to carry out phenotypical characterization of lymphocytes during primary infections on day 3 after birth. infected and noninfected piglets were investigated between days 7 and 16 after birth. lymphocyt ... | 2010 | 20398223 |
| changes in lymphocyte populations in suckling piglets during primary infections with isospora suis. | isospora suis, a common intestinal parasite of piglets, causes neonatal porcine coccidiosis, which results in reduced and uneven weaning weights and economic losses in pig production. nevertheless, there are no detailed studies available on the immune response to i. suis. the aim of this study was to carry out phenotypical characterization of lymphocytes during primary infections on day 3 after birth. infected and noninfected piglets were investigated between days 7 and 16 after birth. lymphocyt ... | 2010 | 20398223 |
| age, not infection dose, determines the outcome of isospora suis infections in suckling piglets. | data from 13 trials involving 124 suckling piglets experimentally infected with isospora suis were evaluated for the effects of infection dose and age on the clinical and parasitological outcome of infection in four different models, infections with 1,000 oocysts on the 1(st) day of life (d.o.l.) (model 1; 9 piglets/3 litters), 1,000 oocysts on the 4(th) d.o.l. (model 2; 25 piglets/11 litters), 1,500 oocysts on the 4(th) d.o.l. (model 3; 40 piglets/20 litters) and 10,000 oocysts on the 4(th) d.o ... | 2009 | 19575237 |
| age, not infection dose, determines the outcome of isospora suis infections in suckling piglets. | data from 13 trials involving 124 suckling piglets experimentally infected with isospora suis were evaluated for the effects of infection dose and age on the clinical and parasitological outcome of infection in four different models, infections with 1,000 oocysts on the 1(st) day of life (d.o.l.) (model 1; 9 piglets/3 litters), 1,000 oocysts on the 4(th) d.o.l. (model 2; 25 piglets/11 litters), 1,500 oocysts on the 4(th) d.o.l. (model 3; 40 piglets/20 litters) and 10,000 oocysts on the 4(th) d.o ... | 2009 | 19575237 |
| porcine coccidiosis--investigations on the cellular immune response against isospora suis. | porcine neonatal coccidiosis is caused by the protozoan isospora suis and affects mainly piglets in the first three weeks of life. high morbidity with diarrhoea and reduced weight gain lead to economic losses, affecting pig-breeding worldwide. infection causes damage of the mucosal surface in the jejunum and ileum and transient non-haemorrhagic diarrhoea. secondary infections with other enteric pathogens may lead to increased mortality. despite its economic and veterinary importance, the immunol ... | 2009 | 19575236 |
| porcine coccidiosis--investigations on the cellular immune response against isospora suis. | porcine neonatal coccidiosis is caused by the protozoan isospora suis and affects mainly piglets in the first three weeks of life. high morbidity with diarrhoea and reduced weight gain lead to economic losses, affecting pig-breeding worldwide. infection causes damage of the mucosal surface in the jejunum and ileum and transient non-haemorrhagic diarrhoea. secondary infections with other enteric pathogens may lead to increased mortality. despite its economic and veterinary importance, the immunol ... | 2009 | 19575236 |
| toltrazuril and sulphonamide treatment against naturally isospora suis infected suckling piglets: is there an actual profit? | a study was carried out to assess the efficacy and the economic profit of prophylactic treatment against isopsora suis with toltrazuril or with a sulfamethazine/trimethoprim combination in piglets from an intensive pig farm. thirty-one litters were included in study. eight litters were treated once with toltrazuril (20 mg/kg b.w.) at 3 days of age (toltra group); 8 litters were treated with 2 ml/animal of a [corrected] sulphonamide combination (sodium sulfamethazine 250 [dosage error corrected] ... | 2009 | 19457615 |
| toltrazuril and sulphonamide treatment against naturally isospora suis infected suckling piglets: is there an actual profit? | a study was carried out to assess the efficacy and the economic profit of prophylactic treatment against isopsora suis with toltrazuril or with a sulfamethazine/trimethoprim combination in piglets from an intensive pig farm. thirty-one litters were included in study. eight litters were treated once with toltrazuril (20 mg/kg b.w.) at 3 days of age (toltra group); 8 litters were treated with 2 ml/animal of a [corrected] sulphonamide combination (sodium sulfamethazine 250 [dosage error corrected] ... | 2009 | 19457615 |
| modified flotation method with the use of percoll for the detection of isospora suis oocysts in suckling piglet faeces. | the modification of flotation method for the examination of diarrhoeic piglet faeces for the detection of isospora suis oocysts was elaborated. the method was based on removing fractions of fat from the sample of faeces by centrifugation with a 25% percoll solution. the investigations were carried out in comparison to the mcmaster method. from five variants of the percoll flotation method, the best results were obtained when 2ml of flotation liquid per 1g of faeces were used. the limit of detect ... | 2008 | 18602760 |
| modified flotation method with the use of percoll for the detection of isospora suis oocysts in suckling piglet faeces. | the modification of flotation method for the examination of diarrhoeic piglet faeces for the detection of isospora suis oocysts was elaborated. the method was based on removing fractions of fat from the sample of faeces by centrifugation with a 25% percoll solution. the investigations were carried out in comparison to the mcmaster method. from five variants of the percoll flotation method, the best results were obtained when 2ml of flotation liquid per 1g of faeces were used. the limit of detect ... | 2008 | 18602760 |
| molecular identification and prevalence of isospora sp. in pigs in western australia using a pcr-rflp assay. | little is known about the prevalence of isospora in domestic pigs in western australia. a total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia. faecal samples were screened using a pcr-rflp assay based on the its-1 rdna locus. an overall prevalence of 10.4% (30/289) was identified. isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-wea ... | 2008 | 18601925 |
| molecular identification and prevalence of isospora sp. in pigs in western australia using a pcr-rflp assay. | little is known about the prevalence of isospora in domestic pigs in western australia. a total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of western australia. faecal samples were screened using a pcr-rflp assay based on the its-1 rdna locus. an overall prevalence of 10.4% (30/289) was identified. isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-wea ... | 2008 | 18601925 |
| survival of isospora suis oocysts under controlled environmental conditions. | isospora suis is a coccidian parasite infecting piglets soon after birth. while the gross epidemiology of i. suis is well known, little knowledge exists on the ecology of the oocysts. to study the development and survival of oocysts of i. suis under controlled laboratory conditions, known numbers of oocysts ( approximately 200 in each of 4 replicates) were exposed to all combinations of 4 relative humidities (53-100% rh) and 3 temperatures (20 degrees , 25 degrees , 30 degrees c). determination ... | 2008 | 18289796 |
| survival of isospora suis oocysts under controlled environmental conditions. | isospora suis is a coccidian parasite infecting piglets soon after birth. while the gross epidemiology of i. suis is well known, little knowledge exists on the ecology of the oocysts. to study the development and survival of oocysts of i. suis under controlled laboratory conditions, known numbers of oocysts ( approximately 200 in each of 4 replicates) were exposed to all combinations of 4 relative humidities (53-100% rh) and 3 temperatures (20 degrees , 25 degrees , 30 degrees c). determination ... | 2008 | 18289796 |
| population dynamics and intra-litter transmission patterns of isospora suis in suckling piglets under on-farm conditions. | the aim of this study was to investigate the intra-litter infection dynamics of isospora suis under natural conditions, and to study any association between parasite transmission and the contamination level of the farrowing pen by applying different interventions in order to reduce the transmission of i. suis infection within the litter. the study was divided in 2 trials including in total 22 litters (254 piglets). the first trial included 4 litters (where standard procedures practiced routinely ... | 2008 | 18021464 |
| population dynamics and intra-litter transmission patterns of isospora suis in suckling piglets under on-farm conditions. | the aim of this study was to investigate the intra-litter infection dynamics of isospora suis under natural conditions, and to study any association between parasite transmission and the contamination level of the farrowing pen by applying different interventions in order to reduce the transmission of i. suis infection within the litter. the study was divided in 2 trials including in total 22 litters (254 piglets). the first trial included 4 litters (where standard procedures practiced routinely ... | 2008 | 18021464 |
| porcine isosporosis: infection dynamics, pathophysiology and immunology of experimental infections. | isospora suis, an intestinal protozoan parasite of swine, is the causative agent of neonatal coccidiosis, a disease with high morbidity in affected pig-breeding units and consequently of high economic importance. infection leads to damage of the mucosal surface in the jejunum and ileum and to non-haemorrhagic diarrhoea. as a result, weight gain of piglets is reduced and secondary infections with other enteric pathogens may lead to increased mortality. despite its economic and veterinary importan ... | 2007 | 17987356 |
| porcine isosporosis: infection dynamics, pathophysiology and immunology of experimental infections. | isospora suis, an intestinal protozoan parasite of swine, is the causative agent of neonatal coccidiosis, a disease with high morbidity in affected pig-breeding units and consequently of high economic importance. infection leads to damage of the mucosal surface in the jejunum and ileum and to non-haemorrhagic diarrhoea. as a result, weight gain of piglets is reduced and secondary infections with other enteric pathogens may lead to increased mortality. despite its economic and veterinary importan ... | 2007 | 17987356 |
| prevalence of isospora suis and eimeria spp. in suckling piglets and sows in poland. | the aim of this investigation was to determine the prevalence of coccidian infections in suckling piglets and sows in poland. the research was carried out in 14 out of 16 polish provinces in the years 2003-2005. the investigation was conducted on three types of farms: large farms (>100 sows), medium farms (25-100 sows) and small farms (<25 sows). diarrhoea of unweaned piglets was observed on all the examined farms. overall, 780 litters of suckling piglets from 104 farms and 267 mother sows were ... | 2007 | 17467906 |
| prevalence of isospora suis and eimeria spp. in suckling piglets and sows in poland. | the aim of this investigation was to determine the prevalence of coccidian infections in suckling piglets and sows in poland. the research was carried out in 14 out of 16 polish provinces in the years 2003-2005. the investigation was conducted on three types of farms: large farms (>100 sows), medium farms (25-100 sows) and small farms (<25 sows). diarrhoea of unweaned piglets was observed on all the examined farms. overall, 780 litters of suckling piglets from 104 farms and 267 mother sows were ... | 2007 | 17467906 |
| amprolium and furazolidone as preventive treatment for intestinal coccidiosis of piglets. | two coccidiostats, amprolium and furazolidone, were used as preventive treatments for intestinal coccidiosis in three-day-old piglets experimentally infected with 50,000 sporulated oocysts of isospora suis.all infected piglets, treated or not, displayed clinical signs compatible with coccidiosis. diarrhea and anorexia appeared around five days postinoculation in the non-treated and in the amprolium-treated groups; these signs were delayed to days 7 and 8 postinoculation in the furazolidone-treat ... | 1987 | 17422910 |
| amprolium and furazolidone as preventive treatment for intestinal coccidiosis of piglets. | two coccidiostats, amprolium and furazolidone, were used as preventive treatments for intestinal coccidiosis in three-day-old piglets experimentally infected with 50,000 sporulated oocysts of isospora suis.all infected piglets, treated or not, displayed clinical signs compatible with coccidiosis. diarrhea and anorexia appeared around five days postinoculation in the non-treated and in the amprolium-treated groups; these signs were delayed to days 7 and 8 postinoculation in the furazolidone-treat ... | 1987 | 17422910 |
| porcine neonatal coccidiosis: evaluation of monensin as preventive therapy. | in this study, we evaluated the efficacy of monensin as a preventive therapy for porcine neonatal coccidiosis. fifteen three-day-old piglets were given 50,000 sporulated oocysts of isospora suis and eight of them received 15 mg/kg of monensin orally every other day. seven piglets served as normal controls. fecal samples were collected and checked for oocyst shedding. at 18 days of age, piglets were euthanized and necropsied.the onset of clinical signs was delayed in the treated group, but all in ... | 1987 | 17422909 |
| porcine neonatal coccidiosis: evaluation of monensin as preventive therapy. | in this study, we evaluated the efficacy of monensin as a preventive therapy for porcine neonatal coccidiosis. fifteen three-day-old piglets were given 50,000 sporulated oocysts of isospora suis and eight of them received 15 mg/kg of monensin orally every other day. seven piglets served as normal controls. fecal samples were collected and checked for oocyst shedding. at 18 days of age, piglets were euthanized and necropsied.the onset of clinical signs was delayed in the treated group, but all in ... | 1987 | 17422909 |
| porcine neonatal coccidiosis in quebec. | intestinal coccidiosis was diagnosed in 110 diarrheic piglets originating from 66 farrowing operations. these piglets had been submitted alive for necropsy early after the beginning of diarrhea, and the diagnosis of coccidiosis was based on the demonstration of coccidial forms in their jejunum by histopathological examination. enteropathogenic strains of escherichia coli were demonstrated in only nine of the 110 piglets while the transmissible gastroenteritis virus and rotavirus were each presen ... | 1982 | 17422160 |
| porcine neonatal coccidiosis in quebec. | intestinal coccidiosis was diagnosed in 110 diarrheic piglets originating from 66 farrowing operations. these piglets had been submitted alive for necropsy early after the beginning of diarrhea, and the diagnosis of coccidiosis was based on the demonstration of coccidial forms in their jejunum by histopathological examination. enteropathogenic strains of escherichia coli were demonstrated in only nine of the 110 piglets while the transmissible gastroenteritis virus and rotavirus were each presen ... | 1982 | 17422160 |
| control of piglet coccidiosis by chemical disinfection with a cresol-based product (neopredisan 135-1). | isospora suis is a common pathogen in piggeries and one of the main causative agents of scours in suckling piglets. besides specific treatment, optimised hygiene including chemical disinfection is considered essential in the control of isosporosis. the suitability of the cresol-based product neopredisan 135-1(r) (np) to inactivate oocysts in vitro and to reduce infection pressure in commercial piggeries was evaluated. under in vitro conditions, np at a final concentration of 2 or 4% induced lysi ... | 2007 | 17364163 |
| control of piglet coccidiosis by chemical disinfection with a cresol-based product (neopredisan 135-1). | isospora suis is a common pathogen in piggeries and one of the main causative agents of scours in suckling piglets. besides specific treatment, optimised hygiene including chemical disinfection is considered essential in the control of isosporosis. the suitability of the cresol-based product neopredisan 135-1(r) (np) to inactivate oocysts in vitro and to reduce infection pressure in commercial piggeries was evaluated. under in vitro conditions, np at a final concentration of 2 or 4% induced lysi ... | 2007 | 17364163 |
| efficacy of various anticoccidials against experimental porcine neonatal isosporosis. | the efficacies of 20 mg/kg body weight (bw) of toltrazuril (toltra) 2 days post-infection (dpi), 2 mg/kg bw of diclazuril 2 and 3 dpi and 200 mg/kg bw of sulphadimidine 2, 3 and 4 dpi were compared in a model for piglet isosporosis. weight gain (first 4 weeks of life) and diarrhoea and oocyst excretion from 4 to 11 dpi were evaluated (10-12 piglets/group). additionally, animals were killed and examined for pathohistological changes of the small intestines 5, 7, 11 and 14 dpi (n = 3 per group and ... | 2007 | 17048000 |
| efficacy of various anticoccidials against experimental porcine neonatal isosporosis. | the efficacies of 20 mg/kg body weight (bw) of toltrazuril (toltra) 2 days post-infection (dpi), 2 mg/kg bw of diclazuril 2 and 3 dpi and 200 mg/kg bw of sulphadimidine 2, 3 and 4 dpi were compared in a model for piglet isosporosis. weight gain (first 4 weeks of life) and diarrhoea and oocyst excretion from 4 to 11 dpi were evaluated (10-12 piglets/group). additionally, animals were killed and examined for pathohistological changes of the small intestines 5, 7, 11 and 14 dpi (n = 3 per group and ... | 2007 | 17048000 |
| [comparison of different methods for examining the feces of suckling piglets for isospora suis]. | a highly sensitive diagnostic method is of great importance for detection of i. suis (isospora suis). in the present study ksfv (combined sedimentation-flotation method) using different flotation solutions (nacl, znso4, nacl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to fv (flotation method) using nacl-sucrose solution and to am (autofluorescence microscopy). ninety faecal samples were examined. using am, oocysts were detected in 83.3% of ... | 2006 | 17009709 |
| [comparison of different methods for examining the feces of suckling piglets for isospora suis]. | a highly sensitive diagnostic method is of great importance for detection of i. suis (isospora suis). in the present study ksfv (combined sedimentation-flotation method) using different flotation solutions (nacl, znso4, nacl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to fv (flotation method) using nacl-sucrose solution and to am (autofluorescence microscopy). ninety faecal samples were examined. using am, oocysts were detected in 83.3% of ... | 2006 | 17009709 |
| isospora suis: an experimental model for mammalian intestinal coccidiosis. | piglets experimentally infected with 10,000 oocysts of isospora suis in three identical trials (n = 50) were examined clinically and coproscopically from 5 to 11 days post-infection (d.p.i.), weighed in weekly intervals until the fourth week of life and compared to age-matched asymptomatic controls (n = 17). furthermore, 17 infected piglets were histologically examined on days 5-14 p.i. infected animals had a significantly lower weight gain than the controls and showed diarrhoea throughout, with ... | 2006 | 16323027 |
| isospora suis: an experimental model for mammalian intestinal coccidiosis. | piglets experimentally infected with 10,000 oocysts of isospora suis in three identical trials (n = 50) were examined clinically and coproscopically from 5 to 11 days post-infection (d.p.i.), weighed in weekly intervals until the fourth week of life and compared to age-matched asymptomatic controls (n = 17). furthermore, 17 infected piglets were histologically examined on days 5-14 p.i. infected animals had a significantly lower weight gain than the controls and showed diarrhoea throughout, with ... | 2006 | 16323027 |
| effects of toltrazuril on the growth of piglets in herds without clinical isosporosis. | isospora suis is a widely prevalent and economically important parasite. the antiprotozoal compound, toltrazuril, was given as a single treatment to piglets without clinical signs in 10 herds. the daily weight gain (dwg) and mortality between the treatment date and weaning was compared on each herd in 10 treated and 10 control litters. the faeces of control litters were examined for oocyst excretion. in six herds, no oocysts were detected and treatment had no effect on dwg or mortality. in four ... | 2007 | 16122955 |
| effects of toltrazuril on the growth of piglets in herds without clinical isosporosis. | isospora suis is a widely prevalent and economically important parasite. the antiprotozoal compound, toltrazuril, was given as a single treatment to piglets without clinical signs in 10 herds. the daily weight gain (dwg) and mortality between the treatment date and weaning was compared on each herd in 10 treated and 10 control litters. the faeces of control litters were examined for oocyst excretion. in six herds, no oocysts were detected and treatment had no effect on dwg or mortality. in four ... | 2007 | 16122955 |
| occurrence of isospora suis in germany, switzerland and austria. | nationwide surveys for the occurrence of isospora suis were carried out in germany, austria and switzerland including a questionnaire regarding herd size, health status and management practices and a coccidiosis sampling kit for pooled faecal samples from litters of suckling piglets. a total of 184 veterinary practices participated in the survey and returned 1745 samples (331 kits) from 324 farms in the north (n = 98), south (n = 84), centre/east (n = 42) and west (n = 10) of germany, austria (n ... | 2005 | 15752269 |
| occurrence of isospora suis in germany, switzerland and austria. | nationwide surveys for the occurrence of isospora suis were carried out in germany, austria and switzerland including a questionnaire regarding herd size, health status and management practices and a coccidiosis sampling kit for pooled faecal samples from litters of suckling piglets. a total of 184 veterinary practices participated in the survey and returned 1745 samples (331 kits) from 324 farms in the north (n = 98), south (n = 84), centre/east (n = 42) and west (n = 10) of germany, austria (n ... | 2005 | 15752269 |
| survey of intestinal parasites in pigs from intensive farms in guangdong province, people's republic of china. | the prevalence of intestinal parasites was investigated in intensive pig farms in guangdong province, china between july 2000 and july 2002. faecal samples from 3636 pigs (both sexes and five age groups) from 38 representative intensive pig farms employing different parasite control strategies were examined for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites using standard techniques. of the 3636 pigs sampled, 209 (5.7%) were infected with trichuris suis, 189 (5.2%) ... | 2004 | 15710534 |
| survey of intestinal parasites in pigs from intensive farms in guangdong province, people's republic of china. | the prevalence of intestinal parasites was investigated in intensive pig farms in guangdong province, china between july 2000 and july 2002. faecal samples from 3636 pigs (both sexes and five age groups) from 38 representative intensive pig farms employing different parasite control strategies were examined for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites using standard techniques. of the 3636 pigs sampled, 209 (5.7%) were infected with trichuris suis, 189 (5.2%) ... | 2004 | 15710534 |
| detection of isospora suis (biester and murray 1934) in piglet faeces--comparison of microscopy and pcr. | 2004 | 15107041 | |
| detection of isospora suis (biester and murray 1934) in piglet faeces--comparison of microscopy and pcr. | 2004 | 15107041 | |
| the role of isospora suis in the ethiology of diarrhoea in suckling piglets. | 2003 | 12928892 | |
| the role of isospora suis in the ethiology of diarrhoea in suckling piglets. | 2003 | 12928892 | |
| studies on the efficacy of toltrazuril, diclazuril and sulphadimidine against artificial infections with isospora suis in piglets. | 2003 | 12928891 | |
| studies on the efficacy of toltrazuril, diclazuril and sulphadimidine against artificial infections with isospora suis in piglets. | 2003 | 12928891 | |
| population biology studies on isospora suis in piglets. | 2003 | 12928890 | |
| population biology studies on isospora suis in piglets. | 2003 | 12928890 | |
| parasitological and morphological findings in porcine isosporosis after treatment with symmetrical triazintriones. | neonatal porcine isosporosis is known to cause serious economic losses in piglet farms by causing severe enteritis with dehydration, weight loss and reduced development in the affected animals, predominantly during the first weeks of life. in the present study, piglets experimentally infected with isospora suis were treated with bay vi 9143, a symmetrical triazintrione, at different days post-infection. as shown by clinical and pathological examinations, bay vi 9143 is effective against the asex ... | 2003 | 12856173 |
| parasitological and morphological findings in porcine isosporosis after treatment with symmetrical triazintriones. | neonatal porcine isosporosis is known to cause serious economic losses in piglet farms by causing severe enteritis with dehydration, weight loss and reduced development in the affected animals, predominantly during the first weeks of life. in the present study, piglets experimentally infected with isospora suis were treated with bay vi 9143, a symmetrical triazintrione, at different days post-infection. as shown by clinical and pathological examinations, bay vi 9143 is effective against the asex ... | 2003 | 12856173 |
| lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with isospora suis. | composition of glycoconjugates was examined in small intestines naturally infected with isospora suis in preweaned pigs by use of 21 biotinylated-labeled lectins with avidin-biotin-peroxidase complex method. as compared with control pig, staining of 18 lectins altered in jejunal villus brush border and goblet cells of pigs naturally infected with i. suis. these results indicate that i. suis infection alters carbohydrate residues on the jejunal intestines. | 2003 | 12679572 |
| lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with isospora suis. | composition of glycoconjugates was examined in small intestines naturally infected with isospora suis in preweaned pigs by use of 21 biotinylated-labeled lectins with avidin-biotin-peroxidase complex method. as compared with control pig, staining of 18 lectins altered in jejunal villus brush border and goblet cells of pigs naturally infected with i. suis. these results indicate that i. suis infection alters carbohydrate residues on the jejunal intestines. | 2003 | 12679572 |
| the role of isospora suis as a pathogen in conventional piglet production in germany. | in order to evaluate the prevalence of isospora suis in conventional piglet production in germany, pooled faecal samples from 327 pig litters from 18 pig production units (20-320 sows each) were examined. at least 10 litters from each farm were investigated. i. suis was present on 83% of the farms and 42.5% of the litters, the infection rate being highest in the third week of age (48.2%). i. suis was found more frequently in samples of diarrhoea than in firm faeces (49.2% compared to 22.2%). twe ... | 2002 | 12069269 |
| the role of isospora suis as a pathogen in conventional piglet production in germany. | in order to evaluate the prevalence of isospora suis in conventional piglet production in germany, pooled faecal samples from 327 pig litters from 18 pig production units (20-320 sows each) were examined. at least 10 litters from each farm were investigated. i. suis was present on 83% of the farms and 42.5% of the litters, the infection rate being highest in the third week of age (48.2%). i. suis was found more frequently in samples of diarrhoea than in firm faeces (49.2% compared to 22.2%). twe ... | 2002 | 12069269 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern germany. | faecal samples from suckling (n = 205) and weaned piglets (n = 82) with diarrhoea from 24 farms in southern germany were examined for shedding of important metazoic parasitic, viral and bacterial pathogens using culture, microscopic and electronmicroscopic methods. escherichia coli isolates were tested further for the enterotoxin genes est-ia and elt-i by colony blot hybridization. isospora suis was diagnosed in 26.9% and cryptosporidium parvum in 1.4% of the piglets investigated. the proportion ... | 2001 | 11315526 |
| prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern germany. | faecal samples from suckling (n = 205) and weaned piglets (n = 82) with diarrhoea from 24 farms in southern germany were examined for shedding of important metazoic parasitic, viral and bacterial pathogens using culture, microscopic and electronmicroscopic methods. escherichia coli isolates were tested further for the enterotoxin genes est-ia and elt-i by colony blot hybridization. isospora suis was diagnosed in 26.9% and cryptosporidium parvum in 1.4% of the piglets investigated. the proportion ... | 2001 | 11315526 |
| pcr-based differentiation of three porcine eimeria species and isospora suis. | isospora suis and eimeria are frequent coccidian parasites of pigs. the unsporulated oocysts of eimeria species and of i. suis are difficult to differentiate. therefore, a species-specific pcr was developed. pcr products were amplified from eimeria polita, eimeria porci, and eimeria scabra using primers from the conserved 18s rrna regions and were subsequently sequenced. based on variable sequence regions, primers were constructed for the differentiation of the three eimeria species and i. suis. ... | 2001 | 11163694 |
| pcr-based differentiation of three porcine eimeria species and isospora suis. | isospora suis and eimeria are frequent coccidian parasites of pigs. the unsporulated oocysts of eimeria species and of i. suis are difficult to differentiate. therefore, a species-specific pcr was developed. pcr products were amplified from eimeria polita, eimeria porci, and eimeria scabra using primers from the conserved 18s rrna regions and were subsequently sequenced. based on variable sequence regions, primers were constructed for the differentiation of the three eimeria species and i. suis. ... | 2001 | 11163694 |