Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| ribosomal protein l18 is an essential factor that promote rice stripe virus accumulation in small brown planthopper. | rice stripe virus (rsv) transmitted by the vector, small brown planthopper (sbph), can cause a severe rice disease. the nucleocapsid (n) protein is the major component of rsv ribonucleoprotein particles (rnps), and it plays important roles in viral persistent-propagative transmission by sbph. to gain further insights into the vector components enabling rsv transmission, a gal4-based yeast two-hybrid system was utilized to find unknown vector factors that interact with the n protein. thirteen dif ... | 2018 | 29374519 |
| maize iranian mosaic virus shows a descending transcript accumulation order in plant and insect hosts. | maize iranian mosaic virus (mimv) is a distinct member of the genus nucleorhabdovirus. in this study, expression of all mimv genes in maize for four weeks after inoculation and in inoculative planthoppers was examined using a quantitative rt-pcr (rt-qpcr) assay. accumulation of mimv p, gene 3, m, g and l transcripts relative to n transcripts was measured and normalized to 18s rrna in maize plants and to the ribosomal protein s13 gene (rps13) in planthoppers using the comparative ct method. in pl ... | 2017 | 29275458 |
| identification of virus-derived sirnas and their targets in rbsdv-infected rice by deep sequencing. | rna interference (rnai) is a conserved mechanism against viruses in plants and animals. it is thought to inactivate the viral genome by producing virus-derived small interfering rnas (vsirnas). rice black-streaked dwarf virus (rbsdv) is transmitted to plants by the small brown planthopper (laodelphax striatellus), and seriously threatens production of rice in east asia, particularly oryza sativa japonica subspecies. through deep sequencing, genome-wide comparisons of rbsdv-derived vsirnas were m ... | 2017 | 29215744 |
| deltamethrin is metabolized by cyp6fu1, a cytochrome p450 associated with pyrethroid resistance in laodelphax striatellus. | cytochrome p450s are known to play a major role in metabolizing a wide range compounds. cyp6fu1 has been over-expressed in a deltamethrin-resistant strain of laodelphax striatellus. this study was conducted to express cyp6fu1 in sf9 cell as a recombinant protein, to confirm its ability in metabolizing deltamethrin, chlorpyrifos, imidacloprid and traditional p450 probing substrates. | 2017 | 29194952 |
| buprofezin is metabolized by cyp353d1v2, a cytochrome p450 associated with imidacloprid resistance in laodelphax striatellus. | cyp353d1v2 is a cytochrome p450 related to imidacloprid resistance in laodelphax striatellus. this work was conducted to examine the ability of cyp353d1v2 to metabolize other insecticides. carbon monoxide difference spectra analysis indicates that cyp353d1v2 was successfully expressed in insect cell sf9. the catalytic activity of cyp353d1v2 relating to degrading buprofezin, chlorpyrifos, and deltamethrin was tested by measuring substrate depletion and analyzing the formation of metabolites. the ... | 2017 | 29186030 |
| cry64ba and cry64ca, two etx/mtx2-type bacillus thuringiensis insecticidal proteins active against hemipteran pests. | genetically modified crops that express insecticidal bacillus thuringiensis (bt) proteins have become a primary approach for control of lepidopteran (moth) and coleopteran (beetle) pests that feed by chewing the plants. however, the sap-sucking insects (hemiptera) are not particularly susceptible to bt toxins. in this study, we describe two cry toxins (cry64ba and cry64ca) from bt strain 1012 that showed toxicity against two important hemipteran rice pests, laodelphax striatellus and sogatella f ... | 2018 | 29150505 |
| investigation of alimentary canal ultrastructure following knockdown of the dicer-2 gene in planthoppers reveals the potential pathogenicity of southern rice black streaked dwarf virus to its insect vector. | an increasing number of studies are suggesting that plant viruses, including southern rice black-streaked dwarf virus (srbsdv), can adversely affect biological characteristics of insect vectors by unknown mechanisms. to study the adverse effect of srbsdv at cellular level on the insect vector, we promoted viral infection by the disruption of the small interfering rna (sirna) pathway. the transmission electron microscopy was utilized to describe the ultrastructural changes that occurred in insect ... | 2017 | 29141205 |
| genome sequence of the small brown planthopper, laodelphax striatellus. | laodelphax striatellus fallén (hemiptera: delphacidae) is one of the most destructive rice pests. l. striatellus is different from 2 other rice planthoppers with a released genome sequence, sogatella furcifera and nilaparvata lugens, in many biological characteristics, such as host range, dispersal capacity, and vectoring plant viruses. deciphering the genome of l. striatellus will further the understanding of the genetic basis of the biological differences among the 3 rice planthoppers. | 2017 | 29136191 |
| resistance monitoring and cross-resistance role of cyp6cw1 between buprofezin and pymetrozine in field populations of laodelphax striatellus (fallén). | monitoring resistance and investigating insecticide resistance mechanisms are necessary for controlling the small brown planthopper, laodelphax striatellus. the susceptibility to four common insecticides of l. striatellus collected from jiangsu, anhui, zhejiang and jilin provinces of china in 2015 was monitored. the results showed that all field populations remained susceptible to chlorpyrifos and thiamethoxam with resistance ratios (rrs) of 2.3- to 9.5 and 1.6- to 3.3, respectively, while the i ... | 2017 | 29116195 |
| combined transcriptomic/proteomic analysis of salivary gland and secreted saliva in three planthopper species. | the planthoppers are piercing-sucking pests that continuously inject saliva into host plants using specialized stylets. however, knowledge on the constituent and function of planthopper saliva proteins was still limited. in this study, the transcriptomic and proteomic approach were adopted to characterize the composition of salivary glands and their secreted saliva in three planthoppers, respectively. gene repertoires of salivary glands in brown planthopper (nilaparvata lugens, bph), white-backe ... | 2018 | 29109049 |
| comparative proteomic analysis of susceptible and resistant rice plants during early infestation by small brown planthopper. | the small brown planthopper (laodelphax striatellus fallén, homoptera, delphacidae-sbph) is one of the major destructive pests of rice (oryza sativa l.). understanding on how rice responds to sbph infestation will contribute to developing strategies for sbph control. however, the response of rice plant to sbph is poorly understood. in this study, two contrasting rice genotypes, pf9279-4 (sbph-resistant) and 02428 (sbph-susceptible), were used for comparative analysis of protein profiles in the l ... | 2017 | 29089949 |
| a simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs. | rice black-streaked dwarf virus (rbsdv), an important rice virus, is transmitted by vector small brown planthopper (sbph) in a persistent manner, but not transovarial transmission. in order to obtain viruliferous sbph nymphs for relevant research, a simple and reliable method was developed, through allowing sbph adults laying eggs on rbsdv-infected rice plants. the results showed the hatching nymphs on diseased plants could early acquire virus, and the virus was detected in 2nd-instar nymphs fro ... | 2018 | 29050971 |
| hormone signaling regulates nymphal diapause in laodelphax striatellus (hemiptera: delphacidae). | diapause is a physiological adaptation that allows an organism to survive adverse environmental conditions. diapause occurs at a specific developmental stage in each species. there are few reports regarding the molecular regulatory mechanism of nymphal diapause in laodelphax striatellus, which is an important graminaceous crop pest. our previous studies identified the conditions for nymphal diapause in this species. here, we combined rna sequencing transcriptomics and quantitative proteomic anal ... | 2017 | 29042683 |
| resistance to cycloxaprid in laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits. | cycloxaprid is a new oxabridged cis-configuration neonicotinoid insecticide, the resistance development potential and underlying resistance mechanism of which were investigated in the small brown planthopper, laodelphax striatellus (fallén), an important agricultural pest of rice. | 2017 | 28991400 |
| resistance irrelevant cyp417a2v2 was found degrading insecticide in laodelphax striatellus. | cytochrome p450 monooxygenases (cyps) usually overexpressed in resistant strain were found involved in oxidative detoxification of insecticides. in this study, an investigation was conducted to confirm if resistance irrelevant cyps which were not overexpressed in resistant strain before, were capable of degrading insecticides. three resistance irrelevant cyps viz. cyp417a2v2, cyp425a1v2, and cyp4dj1 from cyp4 family of laodelphax striatellus were randomly selected for experiments. cyp417a2v2 and ... | 2017 | 28770044 |
| the c-jun n-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication. | no evidence has shown whether insect-borne viruses manipulate the c-jun n-terminal kinase (jnk) signaling pathway of vector insects. using a system comprising the plant virus rice stripe virus (rsv) and its vector insect, the small brown planthopper, we have studied the response of the vector insect's jnk pathway to plant virus infection. we found that rsv increased the level of tumor necrosis factor-α and decreased the level of g protein pathway suppressor 2 (gps2) in the insect vector. the vir ... | 2017 | 28716183 |
| imidacloprid is hydroxylated by laodelphax striatellus cyp6ay3v2. | laodelphax striatellus (fallén) is one of the most destructive pests of rice, and has developed high resistance to imidacloprid. our previous work indicated a strong association between imidacloprid resistance and the overexpression of a cytochrome p450 gene cyp6ay3v2 in a l. striatellus imidacloprid resistant strain (imid-r). in this study, a transgenic drosophila melanogaster line that overexpressed the l. striatellus cyp6ay3v2 gene was established and was found to confer increased levels of i ... | 2017 | 28654199 |
| jinggangmycin-suppressed reproduction in the small brown planthopper (sbph), laodelphax striatellus (fallen), is mediated by glucose dehydrogenase (gdh). | the small brown planthopper (sbph), laodelphax striatellus (fallen), is a serious pest insect of rice, wheat, and maize in china. sbph not only sucks plant sap but also transmits plant disease viruses, causing serious damage. these viruses include rice striped virus disease (rsv disease), black streaked dwarf, and maize rough disease virus. sbph outbreaks are related to the overuse of pesticides in china. some pesticides, such as triazophos, stimulate the reproduction of sbph, but an antibiotic ... | 2017 | 28595925 |
| does resistance to buprofezin improve heat and cold tolerance of laodelphax striatellus (hemiptera: delphacidae)? | there is ample evidence that insecticide resistance causes fitness costs and benefits in pests, while the impact of insecticide resistance on thermotolerance of pests is mostly unclear. the laodelphax striatellus (fallén), is an important rice insect pest, which has developed resistance to buprofezin in china. here, we investigated differences in heat tolerance and cold tolerance among l. striatellus lines with variable buprofezin resistance. the lethal time for 50% of the individuals to die (lt ... | 2017 | 28595288 |
| quantitative analysis of rice stripe virus in a transovarial transmission cycle during the development and reproduction of its vector, laodelphax striatellus. | the amount of rice stripe virus (rsv) maintained through transovarial transmission was analyzed during the development and reproduction of its vector, laodelphax striatellus. reverse transcription quantitative pcr analysis was used to quantify rna expressed from the rsv coat protein (cp) gene as an estimate of rsv content in nymphs and adults of l. striatellus at various developmental stages. the 18s ribosome rna gene of l. striatellus was chosen as the reference for calculating rsv cp expressio ... | 2017 | 28589385 |
| bacterial microbiota in small brown planthopper populations with different rice viruses. | the small brown planthopper (sbph) is an important virus vector, transmitting rice stripe virus (rsv), and rice black-streaked dwarf virus (rbsdv). insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by sbph. here, we investigated endosymbiont communities in non-viruliferous, rsv-infected, and rbsdv-infected sbph populations using illumina 16s rrna gene miseq sequencing. in total, 281,803 effective sequences ... | 2017 | 28471518 |
| development of a simplified rt-pcr without rna isolation for rapid detection of rna viruses in a single small brown planthopper (laodelphax striatellus fallén). | the small brown planthopper (sbph) is an important pest of cereal crops and acts as a transmission vector for multiple rna viruses. rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. reverse transcription polymerase chain reaction (rt-pcr) is a rapid, sensitive and reliable method for virus detection. the traditional rt-pcr contains a rna isolation step and is widely used for virus detection in insect. however, using the traditional rt-pcr for ... | 2017 | 28468626 |
| variable inhibition of zika virus replication by different wolbachia strains in mosquito cell cultures. | mosquito-borne arboviruses are a major source of human disease. one strategy to reduce arbovirus disease is to reduce the mosquito's ability to transmit virus. mosquito infection with the bacterial endosymbiont wolbachia pipientis wmel is a novel strategy to reduce aedes mosquito competency for flavivirus infection. however, experiments investigating cyclic environmental temperatures have shown a reduction in maternal transmission of wmel, potentially weakening the integration of this strain int ... | 2017 | 28446677 |
| wolbachia-induced loss of male fertility is likely related to branch chain amino acid biosynthesis and ilve in laodelphax striatellus. | wolbachia are endosymbionts that infect many species of arthropods and nematodes. wolbachia-induced cytoplasmic incompatibility (ci) is the most common phenotype in affected hosts, involving embryonic lethality in crosses between wolbachia-infected males and uninfected females. the molecular mechanisms underlying this phenomenon are currently unclear. here we examine the molecular correlates of the wolbachia infection in laodelphax striatellus (fallén), an important rice pest, where embryonic le ... | 2017 | 28412513 |
| identification and characterization of laodelphax striatellus (insecta: hemiptera: delphacidae) neutral sphingomyelinase. | the neutral sphingomyelinase (nsmase) 1 homologue gene lssmase was cloned from laodelphax striatellus, a direct sap-sucker and virus vector of gramineous plants, and expressed via a bac to bac baculovirus expression system. the lssmase-enhanced green fluorescent protein fusion protein was located in the endoplasmic reticulum in a similar manner to mammalian nsmase 1. the biochemical properties of lssmase were determined in detail. the optimal ph and temperature for recombinant lssmase were 8 and ... | 2017 | 28374513 |
| multiple atp-binding cassette transporters are involved in insecticide resistance in the small brown planthopper, laodelphax striatellus. | atp-binding cassette (abc) transporters are membrane-bound proteins involved in the movement of various substrates, including drugs and insecticides, across the lipid membrane. demonstration of the role of human abc transporters in multidrug resistance has led to speculation that they might be an important mechanism controlling the fate of insecticides in insects. however, the role of abc transporters in insects remains largely unknown. the small brown planthopper, laodelphax striatellus fallén, ... | 2017 | 28299835 |
| imidacloprid is degraded by cyp353d1v2, a cytochrome p450 overexpressed in a resistant strain of laodelphax striatellus. | cytochrome p450s are associated with the metabolising of a wide range of compounds, including insecticides. cyp353d1v2 has been found to be overexpressed in an imidacloprid-resistant strain of laodelphax striatellus. thus, this study was conducted to express cyp353d1v2 in sf9 cells as a recombinant protein, to assess its ability to metabolise imidacloprid. | 2017 | 28296046 |
| comparative analysis of the transcriptional responses to low and high temperatures in three rice planthopper species. | the brown planthopper (nilaparvata lugens, bph), white-backed planthopper (sogatella furcifera, wbph) and small brown planthopper (laodelphax striatellus, sbph) are important rice pests in asia. these three species differ in thermal tolerance and exhibit quite different migration and overwintering strategies. to understand the underlying mechanisms, we sequenced and compared the transcriptome of the three species under different temperature treatments. we found that metabolism-, exoskeleton- and ... | 2017 | 28214356 |
| adaptability of small brown planthopper to four rice cultivars using life table and population projection method. | in this study, we evaluated the adaptability of the small brown planthopper (sbph), laodelphax striatellus (hemiptera: delphacidae) to four rice cultivars including shengdao13 (sd13), shengdao14 (sd14), shengdao15 (sd15), and zixiangnuo (zxn) using the age-stage, two-sex life table with a simplified method for recording egg production (i.e., every five days vs. daily). the intrinsic rate of increase (r) of the sbph was the highest (0.1067 d-1) on cultivar sd15, which was similar to the rate on s ... | 2017 | 28205522 |
| evolution of rice stripe virus. | rice stripe virus (rsv) is an insect-borne tenuivirus of economical significance. it is endemic to the rice-growing regions of east asia and exhibits more genetic diversity in yunnan province of china. to gain more insights into the molecular epidemiology and evolution of rsv, recombination analyses were conducted and potential events were detected in each of the four rna segments of rsv. bayesian coalescent method was then applied to the time-stamped coding sequences of the cp gene. the nucleot ... | 2017 | 28189616 |
| effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant laodelphax striatella. | the resistance of the small brown planthopper (sbph), laodelphax striatella, to insecticides has been widely found in china, and has posed serious problems to efforts to control the pest. to determine the costs and benefits of resistance, the life tables of chlorpyrifos-resistant and -susceptible strains were constructed at 24 and 30°c. the results showed the resistant sbph (yn-cpf) had lower fitness at 24°c, but slightly higher fitness at 30°c compared to the susceptible sbph. transcriptomic an ... | 2017 | 28187833 |
| molecular characterization and expression pattern of three gaba receptor-like subunits in the small brown planthopper laodelphax striatellus (hemiptera: delphacidae). | ionotropic γ-aminobutyric acid (gaba)-gated chloride channel receptors mediate rapid inhibitory neurotransmission in vertebrates and invertebrates. gaba receptors are well known to be the molecular targets of synthetic insecticides or parasiticides. three gaba receptor-like subunits, lslcch3, lsgrd and ls8916, of the small brown planthopper, laodelphax striatellus (fallén), a major insect pest of crop systems in east asia, had been identified and characterized in this study. all three genes were ... | 2017 | 28187828 |
| rice stripe virus infection alters mrna levels of sphingolipid-metabolizing enzymes and sphingolipids content in laodelphax striatellus. | sphingolipids and their metabolites have been implicated in viral infection and replication in mammal cells but how their metabolizing enzymes in the host are regulated by viruses remains largely unknown. here we report the identification of 12 sphingolipid genes and their regulation by rice stripe virus in the small brown planthopper (laodelphax striatellus fallén), a serious pest of rice throughout eastern asia. according to protein sequence similarity, we identified 12 sphingolipid enzyme gen ... | 2017 | 28130458 |
| transcriptome analysis reveals the response of iron homeostasis to early feeding by small brown planthopper in rice. | it has been documented that planthopper attacks change iron (fe) content of rice plants. to investigate whether planthopper attacks change rice fe homeostasis at the molecular level, the response of rice fe homeostasis to early feeding by small brown planthopper (sbph) was examined by transcriptome profiling. results showed that the concentration of fe and nicotianamine decreased in resistant rice genotype and increased in susceptible rice genotype after attack by sbph. transcriptome profiling s ... | 2017 | 28112511 |
| cross-resistance to three phenylpyrazole insecticides and a2'n mutation detection of gaba receptor subunit in fipronil-resistant laodelphax striatellus (hemiptera: delphacidae). | laodelphax striatellus (fallén) is an important pest of crops in east asia. over the past decade, phenylpyrazole insecticides, which target the insect gaba receptor, have increasingly been used as alternatives against rice planthoppers. | 2017 | 27943653 |
| genome sizes of nine insect species determined by flow cytometry and k-mer analysis. | the flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five hemipterans, and two hymenopterans. among which, the coleopteran lissorhoptrus oryzophilus (kuschel) had the largest genome of 981 mb. the average genome size was 504 mb, suggesting that insects have a moderate-size genome. compared with the insects in other orders, hymenopterans had small genomes, which were averagely about ~200 mb. we found that the genome ... | 2016 | 27932995 |
| resistance monitoring and cross-resistance patterns of three rice planthoppers, nilaparvata lugens, sogatella furcifera and laodelphax striatellus to dinotefuran in china. | three rice planthoppers, brown planthopper, nilaparvata lugens, white-backed planthopper, sogatella furcifera and small brown planthopper, laodelphax striatellus, are important pests of cultivated rice in tropical and temperate asia. they have caused severe economic loss and developed resistance to insecticides from most chemical classes. dinotefuran is the third neonicotinoid which possesses a broad spectrum and systemic insecticidal activity. we determined the susceptibility of dinotefuran to ... | 2016 | 27914544 |
| rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| first report of cheiloneurus exitiosus (perkins, 1906) and helegonatopus dimorphus (hoffer, 1954) (hymenoptera: encyrtidae) from japan, with remarks on their abundance in rice paddies. | encyrtid secondary parasitoids of delphacidae have not been recorded in japan. however, they may play an important role in the rice ecosystem because they can reduce the number of dryinidae, the natural enemies of rice planthoppers. | 2016 | 27660532 |
| multiple cis-acting elements involved in up-regulation of a cytochrome p450 gene conferring resistance to deltamethrin in smal brown planthopper, laodelphax striatellus (fallén). | as well as arising from single point mutations in binding sites or detoxifying enzymes, it is likely that insecticide resistance mechanisms are frequently controlled by multiple genetic factors, resulting in resistance being inherited as a quantitative trait. however, empirical evidence for this is still rare. here we analyse the causes of up-regulation of cyp6fu1, a monoxygenase implicated in resistance to deltamethrin in the rice pest laodelphax striatellus. the 5'-flanking region of this gene ... | 2016 | 27590347 |
| expression pattern and pharmacological characterisation of two novel alternative splice variants of the glutamate-gated chloride channel in the small brown planthopper laodelphax striatellus. | glutamate-gated chloride channels (glucl) mediate fast inhibitory neurotransmission in invertebrate nervous systems. although only one glucl gene was presented in insects, it showed diverse alternative splicing that was speculated could affect channel function and pharmacology. | 2017 | 27302648 |
| artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus. | rice stripe virus (rsv), the causative agent of rice stripe disease, is transmitted by laodelphax striatellus in a persistent-propagative manner. efficient virus acquisition is primary for studies of virus transmission and virus-insect vector interactions. however, under greenhouse conditions, less than 30% of the l. striatellus population, on average, become viruliferous during feeding on rsv-infected plants. here, we explored a method for efficient rsv acquisition by feeding the insects with a ... | 2016 | 27283882 |
| differential proteomics profiling of the ova between healthy and rice stripe virus-infected female insects of laodelphax striatellus. | rice stripe virus-infected females of the small brown planthopper (sbph, laodelphax striatellus) usually lay fewer eggs with a longer hatch period, low hatchability, malformation and retarded or defective development compared with healthy females. to explore the molecular mechanism of those phenomena, we analyzed the differential proteomics profiling of the ova between viruliferous and healthy female insects using an isobaric tag for relative and absolute quantitation (itraq) approach. we obtain ... | 2016 | 27277140 |
| [resistance risk, cross-resistance and biochemical resistance mechanism of laodelphax striatellus to buprofezin]. | in order to investigate the resistance development law and biochemical resistance mechanism of laodelphax striatellus to buprofezin, spraying rice seedlings was used to continuously screen resistant strains of l. striatellus and dipping rice seedlings was applied to determine the toxicity and cross-resistance of l. striatellus to insecticides. after 32-generation screening with buprofezin, l. striatellus developed 168.49 folds resistance and its reality heritability (h2) was 0.11. if the killing ... | 2016 | 27228617 |
| cloning, expression, purification, and insecticidal activity of a novel cry1na3 toxin from bacillus thuringiensis brc-zyr2. | bacillus thuringiensis produces a variety of insecticidal crystal proteins (icps). genome sequencing is a promising strategy for detecting and identifying b. thuringiensis icps, which are of great interest to the biocontrol field. in this study, a novel icp gene was cloned from b. thuringiensis brc-zyr2 based on genomic data from 454 gs-flx titanium sequencing and an analysis of the results using the b. thuringiensis toxin_scanner (http://bcam.hzaubmb.net/bttoxin_scanner/index.php). cry1na3 desi ... | 2016 | 27170729 |
| seasonal changes in the percentage of rice stripe virus viruliferous laodelphax striatellus (hemiptera: delphacidae) in paddy fields in japan. | rice stripe disease, which is caused by rice stripe virus (rsv), is one of the most serious viral diseases of rice. rsv is transmitted in a persistent manner bylaodelphax striatellus(fallén). the incidence of the disease can be estimated from the density of viruliferous vectors. understanding seasonal changes of the percentage of viruliferousl. striatelluscan facilitate forecasting and controlling the disease. in paddies, the percentage of viruliferous insects fluctuated in phase with the rate o ... | 2016 | 27099363 |
| mechanisms of resistance to insecticides targeting rdl gaba receptors in planthoppers. | lindane and cyclodienes, such as dieldrin and α-endosulfan, represent the first generation of noncompetitive antagonists (ncas) against the insect rdl gaba receptor. it has been reported that a2's and a2'g mutations in the membrane-spanning region m2 of the rdl gaba receptor confer resistance to lindane and cyclodienes. fipronil is a second-generation nca, but a2's and a2'g mutations provide a low level of cross-resistance to fipronil. moreover, a novel a2'n mutation that confers fipronil resist ... | 2017 | 27000517 |
| roles of the laodelphax striatellus down syndrome cell adhesion molecule in rice stripe virus infection of its insect vector. | the arthropod down syndrome cell adhesion molecule (dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. to date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. in the present study, we cloned and characterized the cdna of laodelphax striatellus dscam (lsdscam) and investigated the function of lsdscam in rice stripe vir ... | 2016 | 26991800 |
| genetic and biochemical mechanisms of rice resistance to planthopper. | this article presents a comprehensive review on the genetic and biochemical mechanisms governing rice-planthopper interactions, aiming to contribute substantial planthopper control and facilitate breeding for resistance to planthoppers in rice. the rice planthopper is the most destructive pest of rice and a substantial threat to rice production. the brown planthopper (bph), white-backed planthopper (wbph) and small brown planthopper (sbph) are three species of delphacid planthoppers and importan ... | 2016 | 26979747 |
| viruliferous rate of small brown planthopper is a good indicator of rice stripe disease epidemics. | rice stripe virus (rsv), its vector insect (small brown planthopper, sbph) and climatic conditions in jiangsu, china were monitored between 2002 and 2012 to determine key biotic and abiotic factors driving epidemics of the disease. average disease severity, disease incidence and viruliferous rate of sbph peaked in 2004 and then gradually decreased. disease severity of rsv was positively correlated with viruliferous rate of the vector but not with the population density of the insect, suggesting ... | 2016 | 26898155 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| geographic variation of diapause and sensitive stages of photoperiodic response in laodelphax striatellus fallén (hemiptera: delphacidae). | large numbers of the small brown planthopper laodelphax striatellus (fallén) (hemiptera: delphacidae) occur in temperate regions, causing severe losses in rice, wheat, and other economically important crops. the planthoppers enter diapause in the third- or fourth-instar nymph stage, induced by short photoperiods and low temperatures. to investigate the geographic variation in l. striatellus diapause, we compared the incidence of nymphal diapause under various constant temperature (20 and 27 °c) ... | 2016 | 26839318 |
| knockdown of nadph-cytochrome p450 reductase results in reduced resistance to buprofezin in the small brown planthopper, laodelphax striatellus (fallén). | nadph-cytochrome p450 reductase (cpr) plays an important role in cytochrome p450 function, and cpr knockdown in several insects leads to increased susceptibility to insecticides. however, a putative cpr gene has not yet been fully characterized in the small brown planthopper laodelphax striatellus, a notorious agricultural pest in rice that causes serious damage by transmitting rice stripe and rice black-streaked dwarf viruses. the objective of this study was to clone the cdna and to knock down ... | 2016 | 26821654 |
| organ-specific transcriptome response of the small brown planthopper toward rice stripe virus. | rice stripe virus (rsv) causes rice stripe disease and is transmitted by the small brown planthopper (laodelphax striatellus, sbph) in a persistent, circulative, and propagative manner. the alimentary canal and salivary gland of sbph play important roles in viral replication and transmission. however, little is known about the underlying molecular functions of these two organs in the interaction between rsv and sbph. in this study, organ-specific transcriptomes of the alimentary canal and saliva ... | 2016 | 26678499 |
| omics-based comparative transcriptional profiling of two contrasting rice genotypes during early infestation by small brown planthopper. | the small brown planthopper (sbph) is one of the destructive pests of rice. although different biochemical pathways that are involved in rice responding to planthopper infestation have been documented, it is unclear which individual metabolic pathways are responsive to planthopper infestation. in this study, an omics-based comparative transcriptional profiling of two contrasting rice genotypes, an sbph-resistant and an sbph-susceptible rice line, was assessed for rice individual metabolic pathwa ... | 2015 | 26633389 |
| different pathogenicities of rice stripe virus from the insect vector and from viruliferous plants. | persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. however, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. in this study, we used rice stripe virus (rsv), its insect vector laodelphax striatellus and host plant (oryza sativa) to explore how persistent viruses acquire pathogenicity from insect vectors. rsv acquired phytopathogenicity in both the alim ... | 2016 | 26585422 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| identification and profiling of conserved and novel micrornas in laodelphax striatellus in response to rice black-streaked dwarf virus (rbsdv) infection. | micrornas (mirnas) are small non-coding endogenous rna molecules that play important roles in various biological processes. this study examined microrna profiles of laodelphax striatellus using the small rna libraries derived from virus free (vf) and rice black-streaked dwarf virus (rbsdv) infected (rb) insects. a total of 59 mature mirnas (46 mirna families) were identified as conserved insect mirnas in both vf and rb libraries. among these conserved mirnas, 24 were derived from the two arms of ... | 2014 | 26484150 |
| infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems. | himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ... | 2015 | 26470259 |
| differential interactions of 5-(4-piperidyl)-3-isoxazolol analogues with insect γ-aminobutyric acid receptors leading to functional selectivity. | γ-aminobutyric acid (gaba) receptors (gabars) mediate fast inhibitory synaptic transmission and are also targets for drugs and insecticides. to better understand the molecular interactions of ligands with the orthosteric sites of gabars, we examined 4-aryl/arylalkyl-5-(4-piperidyl)-3-isoxazolol, 4-aryl-5-(4-piperidyl)-3-isothiazolol, and 5-aryl-4-(4-piperidyl)-1-hydroxypyrazole for their antagonism with regard to three insect gabars. the 3-isoxazolol was preferable to the 3-isothiazolol and 1-hy ... | 2015 | 26453818 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| the wolbachia wo bacteriophage proteome in the aedes albopictus c/wstr1 cell line: evidence for lytic activity? | wolbachia pipientis (rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. modification of host population structure has potential applications for control of pest species, particularly if wolbachia can be maintained, manipulated, and genetically engineered in vitro. although wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with dist ... | 2015 | 26427709 |
| constitutive overexpression of cytochrome p450 associated with imidacloprid resistance in laodelphax striatellus (fallén). | imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. resistance to imidacloprid has been reported in a field population of laodelphax striatellus. the present work was conducted to study the molecular mechanisms of imidacloprid resistance. | 2016 | 26395964 |
| susceptibility of sogatella furcifera and laodelphax striatellus (hemiptera: delphacidae) to six insecticides in china. | the whitebacked planthopper, sogatella furcifera (horváth), and small brown planthopper, laodelphax striatellus (fallén), both are important crop pests throughout china, especially in rice. application of chemical insecticides is the major control practice. consequently, insecticide resistance has become an urgent issue. in this study, resistance levels to six conventional insecticides were evaluated for these two species collected from major occurring areas of china. additionally, imidacloprid- ... | 2014 | 26309282 |
| rice stripe virus counters reduced fecundity in its insect vector by modifying insect physiology, primary endosymbionts and feeding behavior. | virus-vector relationships can be complex and diverse as a result of long-term coevolution. understanding these interactions is crucial for disease and vector management. rice stripe virus (rsv) is known to be transovarially transmitted within its vector, laodelphax striatellus, and causes serious rice stripe disease. in rsv-infected l. striatellus, we found contrasting changes in vector fecundity, physiology, primary endosymbionts (i.e. yeast-like symbionts, yls) and feeding behavior that can i ... | 2015 | 26211618 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein. | numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ... | 2015 | 26091699 |
| [stress effects of imidacloprid on rsv in rice plants]. | the rice stripe disease is a viral disease transmitted by small brown planthopper, laodelphax striatellus, which outbroke a few years ago in the yangtze river basin, especially jiangsu region, china. to study the effects of imidacloprid stress on rice stripe virus (rsv) in rice plants, the rice seedlings were treated with imidacloprid 1, 2, 3 and 4 times (b1, b2, b3 and b4), respectively, after artificial inoculation by l. striatellus for 48 h, and the expression levels of relative genes includi ... | 2014 | 25876413 |
| knockdown of the ionotropic γ-aminobutyric acid receptor (gabar) rdl gene decreases fipronil susceptibility of the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | insect γ-aminobutyric acid receptors (gabars) are important molecular targets of cyclodiene and phenylpyrazole insecticides. previously gabars encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. in this study, we cloned the open reading frame cdna sequence of rdl gene from fipronil-susceptible and fipronil-resistant strains of laodelphax striatellus (lsrdl). sequence analysis confirmed the pre ... | 2015 | 25808850 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| take-off time of the first generation of the overwintering small brown planthopper, laodelphax striatellus in the temperate zone in east asia. | overseas migration of the small brown planthopper, laodelphax striatellus (fallén), occurs during the winter wheat harvest season in east asia. knowing the take-off time of emigrating l. striatellus is crucial for predicting such migrations with a simulation technique because winds, carriers of migratory insects, change continuously. several methods were used in china and japan from late may to early june 2012 and again in 2013 to identify the precise timing of take-off. these methods included: ... | 2015 | 25780936 |
| cytochrome p450 cyp4de1 and cyp6cw3v2 contribute to ethiprole resistance in laodelphax striatellus (fallén). | laodelphax striatellus fallén (hemiptera: delphacidae), a destructive pest of rice, has developed high resistance to multiple insecticides, threatening the success of pest management programmes. the present study investigated ethiprole resistance mechanisms in a field population that is highly resistant to ethiprole. that population was used to establish a laboratory population that was subjected to further selection to produce a resistant strain. target genes were cloned and compared between th ... | 2015 | 25693611 |
| study of the long-distance migration of small brown planthoppers laodelphax striatellus in china using next-generation sequencing. | the small brown planthopper (laodelphax striatellus) is a widespread insect pest of rice in east asia. previous studies have shown the long-distance migrations undertaken by l. striatellus, but have not provided molecular evidence to support this. | 2016 | 25684265 |
| rice stripe tenuivirus nonstructural protein 3 hijacks the 26s proteasome of the small brown planthopper via direct interaction with regulatory particle non-atpase subunit 3. | the ubiquitin/26s proteasome system plays a vital role in regulating host defenses against pathogens. previous studies have highlighted different roles for the ubiquitin/26s proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. in this study, we determined that the 26s proteasome is present in the small brown planthopper (sbph) (laodelphax striatellus) and has components similar to those in plants and ... | 2015 | 25653432 |
| evidence for high dispersal ability and mito-nuclear discordance in the small brown planthopper, laodelphax striatellus. | understanding dispersal ability in pest species is critical for both theoretical aspects of evolutionary and population biology and from a practical standpoint, such as implementing effective forecasting systems. the small brown planthopper (sbph), laodelphax striatellus (fallén), is an economically important pest, but few data exist on its dispersal ability. here, we used mitochondrial and nuclear markers to elucidate the population genetic structure of sbph and of the parasitic bacterium wolba ... | 2015 | 25622966 |
| rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos. | plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ... | 2015 | 25601039 |
| pichia anomala, a new species of yeast-like endosymbionts and its variation in small brown planthopper (laodelphax striatellus). | yeast-like symbionts (yls) are endosymbionts that promote the growth of delphacid planthoppers (hemiptera: delphacidae), some of which are pests on cultivated rice. identification and characterization of yls growth can be helpful for pest control, because it has been demonstrated that there is a variety of yls in rice planthopper and they affected the planthopper's growth and virulence to plant hosts. so, elucidation of the species of yls in planthopper is crucial for exploiting a new way to con ... | 2015 | 25499750 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| effects of two pesticides, tzp and jgm, on reproduction of three planthopper species, nilaparvata lugens stål, sogatella furcifera horvath, and laodelphax striatella fallén. | three planthopper species, the brown planthopper (bph), nilaparvata lugens stål, the white-backed planthopper (wbph), sogatella furcifera horvath, and the small brown planthopper (sbph), laodelphax striatella fallén, often co-occur in rice grown regions of china. the present investigation examined effects of two pesticides, triazophos (tzp) and jinggangmycin (jgm) (a fungicide), on reproduction of bph, wbph, and sbph. the results showed that tzp stimulated the fecundity of the three planthopper ... | 2014 | 25307466 |
| transcriptome analysis of the small brown planthopper, laodelphax striatellus carrying rice stripe virus. | rice stripe virus (rsv), the type member of the genus tenuivirus, transmits by the feeding behavior of small brown planthopper (sbph), laodelphax striatellus. to investigate the interactions between the virus and vector insect, total rna was extracted from rsv-viruliferous sbph (rvls) and non-viruliferous sbph (nvls) adults to construct expressed sequence tag databases for comparative transcriptome analysis. over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of ... | 0 | 25288960 |
| diapause induction and termination in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | the small brown planthopper, laodelphax striatellus (fallén) enters the photoperiodic induction of diapause as 3rd or 4th instar nymphs. the photoperiodic response curves in this planthopper showed a typical long-day response type with a critical daylength of approximately 11 h at 25 °c, 12 h at 22 and 20 °c and 12.5 h at 18 °c, and diapause induction was almost abrogated at 28 °c. the third stage was the most sensitive stage to photoperiod. the photoperiodic response curve at 20 °c showed a gra ... | 2014 | 25188306 |
| proteomic profiling of a robust wolbachia infection in an aedes albopictus mosquito cell line. | wolbachia pipientis, a widespread vertically transmitted intracellular bacterium, provides a tool for insect control through manipulation of host-microbe interactions. we report proteomic characterization of wstr, a wolbachia strain associated with a strong cytoplasmic incompatibility phenotype in its native host, laodelphax striatellus. in the aedes albopictus c/wstr1 mosquito cell line, wstr maintains a robust, persistent infection. ms/ms analyses of gel bands revealed a protein 'footprint' do ... | 2014 | 25155417 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| identification and characterization of micrornas in small brown planthopper (laodephax striatellus) by next-generation sequencing. | micrornas (mirnas) are endogenous non-coding small rnas that regulate gene expression at the post-transcriptional level and are thought to play critical roles in many metabolic activities in eukaryotes. the small brown planthopper (laodephax striatellus fallén), one of the most destructive agricultural pests, causes great damage to crops including rice, wheat, and maize. however, information about the genome of l. striatellus is limited. in this study, a small rna library was constructed from a ... | 2014 | 25057821 |
| bio-effects of near-zero magnetic fields on the growth, development and reproduction of small brown planthopper, laodelphax striatellus and brown planthopper, nilaparvata lugens. | magnetic fields markedly affect the growth and development of many species of organisms potentially due to cryptochrome and endogenous presence of magnetic materials. sensitivity to magnetic fields can also be involved in geomagnetic orientation by some long-distance migratory insects. in this study, near-zero magnetic fields (nzmf) in relation to normal geomagnetic fields (gmf) were setup using the hypomagnetic field space system (hmfs) to investigate the effects of magnetic fields on the growt ... | 2014 | 24995837 |
| molecular cloning and characterization of the putative halloween gene phantom from the small brown planthopper laodelphax striatellus. | ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in insect postembryonic development and reproduction. several cytochrome p450 mono-oxygenases (cyps), encoded by the halloween genes, have been documented to be involved in ecdysteroidogenesis in representative insects in diptera, lepidoptera and orthoptera. here the putative halloween gene phantom (phm, cyp306a1) from a hemipteran insect species, the small brown planthopper laodelphax striatellus, was cloned. lsphm shows five insect ... | 2015 | 24954278 |
| depletion of host cell riboflavin reduces wolbachia levels in cultured mosquito cells. | wolbachia is an obligate intracellular alphaproteobacterium that occurs in arthropod and nematode hosts. wolbachia presumably provides a fitness benefit to its hosts, but the basis for its retention and spread in host populations remains unclear. wolbachia genomes retain biosynthetic pathways for some vitamins, and the possibility that these vitamins benefit host cells provides a potential means of selecting for wolbachia-infected cell lines. to explore whether riboflavin produced by wolbachia i ... | 2014 | 24789726 |
| a halloween gene shadow is a potential target for rna-interference-based pest management in the small brown planthopper laodelphax striatellus. | laodelphax striatellus is an economically important rice pest in china. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. the cytochrome p450 monooxygenase shadow (sad) plays a critical role in ecdysteroidogenesis. here, tests were conducted to establish whether lssad was a potential target gene for rna-interference-based management of l. striatellus. | 2015 | 24648012 |
| transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector. | most plant viruses are transmitted by hemipteroid insects. some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. rice stripe virus (rsv), a tenuivirus, transmitted mainly by the small brown planthopper (laodelphax striatellus), is also spread to the offspring through the eggs. here, we used the rsv-planthopper system as a model to investigate the mechanism of transovarial transmission and demonst ... | 2014 | 24603905 |
| differential mechanisms of action of the novel γ-aminobutyric acid receptor antagonist ectoparasiticides fluralaner (a1443) and fipronil. | fluralaner (a1443) is an isoxazoline ectoparasiticide that is a novel antagonist of γ-aminobutyric acid (gaba) receptors (gabars), with a potency comparable to that of fipronil, a phenylpyrazole ectoparasiticide. to clarify the biological effectiveness of fluralaner against fipronil-resistant pests, differences in the actions of fluralaner and fipronil on gabars that possess resistance to dieldrin (rdl)-type mutations were evaluated. | 2015 | 24591229 |
| nonstructural protein ns4 of rice stripe virus plays a critical role in viral spread in the body of vector insects. | rice stripe virus (rsv), a tenuivirus, is transmitted by small brown planthopper (sbph) in a persistent-propagative manner. in this study, sequential infection of rsv in the internal organs of sbph after ingestion of virus indicated that rsv initially infected the midgut epithelium, and then progressed to the visceral muscle tissues, through which rsv spread to the entire alimentary canal. finally, rsv spread into the salivary glands and reproductive system. during viral infection, the nonstruct ... | 2014 | 24523924 |
| small brown planthopper resistance loci in wild rice (oryza officinalis). | host-plant resistance is the most practical and economical approach to control the rice planthoppers. however, up to date, few rice germplasm accessions that are resistant to the all three kinds of planthoppers (1) brown planthopper (bph; nilaparvata lugens stål), (2) the small brown planthopper (sbph; laodelphax striatellus fallen), and (3) the whitebacked planthopper (wbph, sogatella furcifera horvath) have been identified; consequently, the genetic basis for host-plant broad spectrum resistan ... | 2014 | 24504629 |
| rna interference depletion of the halloween gene disembodied implies its potential application for management of planthopper sogatella furcifera and laodelphax striatellus. | sogatella furcifera and laodelphax striatellus are economically important rice pests in china by acting as vectors of several rice viruses, sucking the phloem sap and blocking the phloem vessels. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. a cytochrome p450 monooxygenase cyp302a1 (22-hydroxylase), encoded by the halloween gene disembodied (dib), plays a critical role in ecdysteroidogenesis. the objective of this study is to test whether dib genes are pot ... | 2014 | 24489765 |
| comparative transcriptome analysis of two rice varieties in response to rice stripe virus and small brown planthoppers during early interaction. | rice stripe, a virus disease, transmitted by a small brown planthopper (sbph), has greatly reduced production of japonica rice in east asia, especially in china. although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance. by de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to sbph and rsv infection in rice. respectively 648 and 937 degs were detect ... | 2013 | 24358146 |
| feeding-induced interactions between nilaparvata lugens and laodelphax striatellus (hemiptera: delphacidae): effects on feeding behavior and honeydew excretion. | using electrical penetration graph, salivary flange, and honeydew measurement, this study investigated the effects of feeding-induced intra- and interspecific interactions on feeding behavior and honeydew excretion of brown planthopper (nilaparvata lugens) compared with small brown planthopper (laodelphax striatellus). results showed that many measures of feeding behavior were affected by feeding-induced intra- and interspecific interactions on two different rice varieties. there were significan ... | 2013 | 24331608 |
| overexpression of multiple detoxification genes in deltamethrin resistant laodelphax striatellus (hemiptera: delphacidae) in china. | the small brown planthopper (sbph), laodelphax striatellus (fallén), is one of the major rice pests in asia and has developed resistance to multiple classes of insecticides. understanding resistance mechanisms is essential to the management of this pest. biochemical and molecular assays were performed in this study to systematically characterize deltamethrin resistance mechanisms with laboratory-selected resistant and susceptible strains of sbph. | 2013 | 24324548 |
| unusual characteristics of dicistrovirus-derived small rnas in the small brown planthopper, laodelphax striatellus. | in this study, sequences of small rna (srna) libraries derived from the insect vector laodelphax striatellus were assembled into contigs and used as queries for database searches. a large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi p virus (hipv). interestingly, hipv-derived srnas had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. hipv srnas had a strong bias towa ... | 2014 | 24323637 |