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biochemical and phylogenetic characterization of the dutpase from the archaeal virus sirv.the derived amino acid sequence from a 474-base pair open reading frame in the genome of the sulfolobus islandicus rod-shaped virus sirv shows striking similarity to bacterial dctp deaminases and to dutpases from eukaryotes, bacteria, poxviridae, and retroviridae. the putative gene was expressed in escherichia coli, and dutpase activity of the recombinant enzyme was demonstrated by hydrolysis of dutp to dump. deamination of dctp by the enzyme was not detected. phylogenetic analysis based on amin ...19989497317
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