Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| molecular detection of pathogens in ticks infesting cattle in nampula province, mozambique. | ticks are ectoparasites that can act as vectors of a large number of pathogens in wild and domestic animals, pets, and occasionally humans. the global threat of emerging or re-emerging tick-borne diseases supports the need for research focused in the zoonotic transmission, especially in countries like mozambique where rural populations are in close contact with domestic animals. the present study aims to: (1) identify tick species infesting cattle from monapo and nacala porto, districts of nampu ... | 2017 | 28856544 |
| identification of anaplasma ovis appendage-associated protein (aaap) for development of an indirect elisa and its application. | ovine anaplasmosis is a tick-borne disease that is caused by anaplasma ovis in sheep and goats. the pathogen is widely distributed in tropical and subtropical regions of the world. at present, diagnosis of the disease mainly depends on microscopy or nucleic acid based molecular tests, although a few serological tests have been applied for the detection of a. ovis infection. | 2017 | 28754151 |
| molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock. | anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ... | 2017 | 28678004 |
| molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france. | corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ... | 2017 | 28645313 |
| molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake victoria and lake baringo, kenya. | although diverse tick-borne pathogens (tbps) are endemic to east africa, with recognized impact on human and livestock health, their diversity and specific interactions with tick and vertebrate host species remain poorly understood in the region. in particular, the role of reptiles in tbp epidemiology remains unknown, despite having been implicated with tbps of livestock among exported tortoises and lizards. understanding tbp ecologies, and the potential role of common reptiles, is critical for ... | 2017 | 28620610 |
| validation of an improved anaplasma antibody competitive elisa for detection of anaplasma ovis antibody in domestic sheep. | an accurate and simple-to-perform new version of a competitive elisa (celisa) kit that became commercially available in 2015 for testing of cattle for antibody to anaplasma marginale was validated for detection of anaplasma ovis antibody in domestic sheep. true positives and negatives were identified using nested pcr (npcr) as the gold standard. negative bovine control sera supplied with the kit were used to calculate % inhibition (%i), designated bovine control elisa (bcelisa), and this was com ... | 2017 | 28480803 |
| prevalence of tick-borne haemoparasites in small ruminants in turkey and diagnostic sensitivity of single-pcr and rlb. | tick-borne haemoparasitic diseases (tbhds), caused by theileria, babesia, anaplasma and ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. many of these diseases threaten livestock production and some also represent a concern to human public health. the primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) ... | 2017 | 28449722 |
| molecular evidence of tick-borne hemoprotozoan-parasites (theileria ovis and babesia ovis) and bacteria in ticks and blood from small ruminants in northern algeria. | using qpcr, standard pcr and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from souk ahras, algeria. borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. anaplasma ovis was screened in (38/73, 52%) rhipicephalus bursa and (5/22, 22.7%) r. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. coxiella burnetii tested positive in r. bursa (4/73, 5.5%) a ... | 2017 | 28131376 |
| a loop-mediated isothermal amplification (lamp) assay targeting 16s rrna gene for rapid detection of anaplasma phagocytophilum infection in sheep and goats. | anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (hga) in humans and tick-borne fever in various kinds of animals. in the present study, a loop-mediated isothermal amplification (lamp) assay for rapid detection of a. phagocytophilum was developed using primers specific to 16s rrna gene of this organism. the lamp assay was performed at 65 c for 60 min and terminated at 80 c for 10 min. the optimal reaction conditions, under which no cross ... | 2017 | 28118097 |
| guidelines for the direct detection of anaplasma spp. in diagnosis and epidemiological studies. | the genus anaplasma (rickettsiales: anaplasmataceae) comprises obligate intracellular gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: anaplasma bovis, anaplasma centrale, anaplasma marginale, anaplasma phagocytophilum, anaplasma platys, and anaplasma ovis. these have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. a zoonotic role has been recognized for a. phagocytophilum, but other spe ... | 2017 | 28055579 |
| anaplasma species of veterinary importance in japan. | anaplasma species of the family anaplasmataceae, order rickettsiales are tick-borne organisms that can cause disease in animals and humans. in japan, all recognized species of anaplasma (except for anaplasma ovis) and a potentially novel anaplasma sp. closely related to anaplasma phagocytophilum have been reported. most of these detected tick-borne pathogens are believed to be lowly pathogenic in animals in japan although the zoonotic a. phagocytophilum has recently been reported to cause clinic ... | 2016 | 27956767 |
| evaluating an indirect rmpsp enzyme-linked immunosorbent assay for the detection of bovine theileria infection in china. | bovine theileriosis, a tick-borne protozoan disease caused by theileria annulata, theileria orientalis and theileria sinensis, is widespread in china and is a serious economic problem for the chinese livestock industry. in this study, recombinant major piroplasma surface proteins (mpsp) of t. annulata, t. orientalis and t. sinensis based on mpsp genes were expressed in escherichia coli bl21(de3). the immunogenicity and specificity of the three purified recombinant mpsp proteins were evaluated wi ... | 2017 | 27942962 |
| molecular detection and genetic characterization of babesia, theileria and anaplasma amongst apparently healthy sheep and goats in the central region of turkey. | babesia spp., theileria spp. and anaplasma spp. are significant tick-borne pathogens of livestock globally. in this study, we investigated the presence and distribution of babesia ovis, theileria ovis and anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the central anatolia region of turkey using species-specific pcr assays. the pcr were conducted using the primers based on the b. ovis ssu rrna (bossurrna), t. ovis ssu rrna (tossurrna) and a. ovis major surface pro ... | 2017 | 27908771 |
| natural anaplasmataceae infection in rhipicephalus bursa ticks collected from sheep in the french basque country. | rhipicephalus bursa is one of 79 species of the genus rhipicephalus in the family of ixodidae. in this study, we investigated anaplasmataceae bacteria associated with r. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as r. bursa, all females were partially engorged. we found that 50% of the ticks were positive in the initial anaplasmataceae qpcr screening. bacterial species ... | 2017 | 27666778 |
| molecular study on infection rates of anaplasma ovis and anaplasma marginale in sheep and cattle in west-azerbaijan province, iran. | this study was carried out to determine the presence and frequency of anaplasma ovis and anaplasma marginale in sheep and dairy cattle in west-azerbaijan province, iran. a total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). the extracted dna from blood cells was screened using genus-specific (anaplasma spp.) nested-polymerase chain reaction (pcr) based on 16s rrna gene primer sets. species-specific pcr was set up us ... | 2016 | 27482362 |
| molecular and phylogenetic analysis of anaplasma spp. in sheep and goats from six provinces of china. | members of the genus anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. here, we investigated the presence of anaplasma spp. in 621 sheep and 710 goats from six provinces of china. polymerase chain reaction (pcr) and dna sequencing were conducted to determine the prevalence of anaplasma (a.) phagocytophilum, a. ovis and a. bovis targeting the 16s ribosomal rna or the major surface protein 4 gene. pcr revealed anaplasma in 39.0% (24 ... | 2016 | 27456776 |
| anaplasma, ehrlichia and rickettsial pathogens in ixodid ticks infesting cattle and sheep in western oromia, ethiopia. | although ticks are widely distributed in all agro-ecological zones of ethiopia, information on tick-borne pathogens is scarce. this study was conducted to determine the presence of anaplasma spp., ehrlichia spp., and rickettsia spp. in rhipicephalus evertsi and rhipicephalus (boophilus) decoloratus collected from cattle and sheep at bako, western oromia, ethiopia, using polymerase chain reaction and sequencing. anaplasma ovis and anaplasma spp., ehrlichia ruminantium and ehrlichia spp. were dete ... | 2016 | 27411938 |
| anaplasma marginale and theileria annulata in questing ticks from portugal. | ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ... | 2016 | 27394441 |
| evaluation of oxidant/antioxidant status, trace mineral levels, and erythrocyte osmotic fragility in goats naturally infected with anaplasma ovis. | anaplasma ovis, an arthropod-borne pathogen that infects erythrocytes, is the major cause of ovine and caprine anaplasmosis. this study was performed to assess in goats infected with a. ovis the osmotic fragility of erythrocytes, antioxidant status, and serum levels of microminerals. blood samples were collected from 104 mixed breed goats in ahvaz area, southwest iran and subjected to parasitologic, hematologic, oxidant/antioxidant, and micromineral assessment. anaplasma infection was detected i ... | 2016 | 27142027 |
| analysis of risk factors and prevalence of haemoplasma infection in dogs. | mycoplasma haemocanis (mhc) and 'candidatus mycoplasma haematoparvum' (cmhp) are canine haemoplasma species that can induce anaemia in immunocompromised and/or splenectomised dogs. this study aimed to determine the prevalence and phylogeny of canine haemoplasma species in dogs from nigeria and describe any risk factors for infection. canine haemoplasma species-specific and generic haemoplasma qpcr assays were used. the species-specific qpcr assays found mhc infection in 18 of 245 dogs (7.3%), an ... | 2016 | 27084481 |
| first molecular evidence for the presence of anaplasma dna in milk from sheep and goats in china. | anaplasmosis, a disease caused by bacteria in the genus of anaplasma, imposes economic constraints on animal breeders and also threatens human health. in the present study, we investigated the presence of anaplasma spp. dna in milk collected from sheep and goats in china. a total of 120 milk samples and 414 field-sampled blood specimens from sheep and goats were analyzed by pcr and dna sequencing. anaplasma ovis was detected in 12 milk samples (three from sheep and nine from goats). the blood sp ... | 2016 | 27038248 |
| evidence of co-infection with mycobacterium bovis and tick-borne pathogens in a naturally infected sheep flock. | ticks are responsible for the transmission of pathogens of veterinary importance, including those affecting sheep. the current study was designed to investigate co-infections with tick-borne and other pathogens in a naturally infected sheep flock with poor health condition using serology and pcr. infection with anaplasma ovis was detected by serology and pcr in 56% of the animals. the presence of rickettsia spp. of the spotted fever group (sfg) was detected by pcr and sequence analysis in 31% of ... | 2016 | 26726806 |
| development of an immunochromatographic strip for the serodiagnosis of theileria infection in sheep. | theileria uilenbergi and t. luwenshuni are tick-borne protozoan parasites, transmitted by haemaphysalis qinghaiensis and h. longicornis, respectively. they are the main causative agents of theileriosis in small ruminants in china. the disease has resulted in severe economic losses and hindered the development of sheep and goat husbandry industry in the endemic regions. | 2015 | 26631226 |
| anaplasmataceae in wild ungulates and carnivores in northern spain. | wild vertebrates are essential hosts for tick-borne diseases but data on the prevalence and diversity of anaplasma spp. in wildlife are scarce. in this study, we used real-time pcr to investigate the distribution of anaplasma species in spleen samples collected from 625 wild animals (137 cervids, 227 wild boar, and 261 carnivores) in two regions in northern spain. a first generic real-time pcr assay was used to screen for the presence of anaplasma spp. followed by a second species-specific multi ... | 2016 | 26596894 |
| serological investigation for west nile virus, anaplasma ovis and leishmania infantum in greek cattle. | this study investigates the seroprevalence of greek adult cattle against west nile virus, anaplasma ovis and lehismania infantum. in total, 156 serum samples were examined, drawn from cattle between 2-4 years old. all the examined cattle originated from slaughterhouses of 4 prefectures in northern greece (thessaloniki, pella, chalkidiki, kilkis), in 2 of which (thessaloniki, pella) human cases of west nile virus had been recorded some months before. thirty out of the 156 (18.6%) samples have tes ... | 2015 | 26455373 |
| molecular survey of anaplasma species in small ruminants reveals the presence of novel strains closely related to a. phagocytophilum in tunisia. | a survey of anaplasma species in small ruminants is still lacking in north african countries. in this study, the presence of a. phagocytophilum, a. phagocytophilum-related species, and a. ovis was investigated in a total of 563 healthy small ruminants (303 goats and 260 sheep), from 25 randomly selected flocks sampled in tunisia. anaplasma spp. and a. ovis overall infection rates were 95.0% and 93.8% in sheep and 69.6% and 65.3% in goats, respectively. a. phagocytophilum was not detected in any ... | 2015 | 26394065 |
| human infection with a novel tick-borne anaplasma species in china: a surveillance study. | anaplasma phagocytophilum and anaplasma ovis cause human infections. we investigated the potential for human pathogenicity of a newly discovered anaplasma species infecting goats in china. | 2015 | 25833289 |
| molecular survey and sequence analysis of anaplasma spp. in cattle and ticks in a malaysian farm. | this study was conducted to determine the occurrence of anaplasma spp. in the blood samples of cattle, goats, deer and ticks in a malaysian farm. using polymerase chain reaction (pcr) and sequencing approach, anaplasma spp. was detected from 81(84.4%) of 96 cattle blood samples. all blood samples from 23 goats and 22 deer tested were negative. based on the analysis of the anaplasma partial 16s ribosomal rna gene, four sequence types (genotypes 1 to 4) were identified in this study. genotypes 1-3 ... | 2014 | 25776603 |
| development of a new pcr-based assay to detect anaplasmataceae and the first report of anaplasma phagocytophilum and anaplasma platys in cattle from algeria. | bovine anaplasmosis is a hemoparasitic disease considered as a major constraint to cattle production in many countries. this pathology is at least partially caused by anaplasma phagocytophilum, anaplasma marginale, anaplasma centrale, and anaplasma bovis. the global threat and emergence of these species in animals require the reliable identification of these bacteria in animal samples. in this study, we developed a new qpcr tool targeting the 23s rrna gene for the detection of anaplasmataceae ba ... | 2015 | 25748051 |
| molecular survey of anaplasma and ehrlichia of red deer and sika deer in gansu, china in 2013. | anaplasma and ehrlichia are important emerging tick-borne pathogens in both humans and animals. here, we conducted a molecular surveillance study in gansu, china to assess the prevalence of anaplasma and ehrlichia spp. in red deer and sika deer based on polymerase chain reaction (pcr) analysis and sequencing of 16s rrna or msp genes. pcr revealed that the prevalence of anaplasma ovis, anaplasma bovis and anaplasma platys of the qilian mountain samples was 32%, 9% and 9%, respectively; the preval ... | 2016 | 25660960 |
| theileria sp. ot3 and other tick-borne pathogens in sheep and ticks in italy: molecular characterization and phylogeny. | pcr reverse line blot (rlb) hybridization and sequencing were used to determine the dynamics of infection with tick-borne pathogens in one hundred apparently healthy sheep in italy. blood samples were tested once prior to the onset of the grazing season (june 2010) and once after the end of the grazing season (august 2010). ticks collected from sheep and from the vegetation were also tested by pcr/rlb. before grazing, 56% of the sheep harbored several tick-borne pathogens: anaplasma ovis was the ... | 2015 | 25448422 |
| risks of suffering tick-borne diseases in sheep translocated to a tick infested area: a laboratory approach for the investigation of an outbreak. | this study was designed to investigate an outbreak of high mortality that occurred in naïve assaf sheep introduced into a latxa sheep flock in the basque country, a region where piroplasmosis is endemic. to identify the causes of this outbreak, a panel of different methods, including traditional pathological, biopathological and parasitological analyses combined with recently developed molecular methods, was used. these novel molecular methods included a multiplex real-time pcr assay to screen f ... | 2015 | 25257849 |
| a molecular survey of anaplasma spp., rickettsia spp., ehrlichia canis and babesia microti in foxes and fleas from sicily. | fleas (insecta: siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. several species have been implicated in pathogen transmission. this study aimed to monitor red foxes and the fleas isolated from them in the palermo and ragusa provinces of sicily, italy, as these organisms are potential reservoirs and vectors of pathogens. thirteen foxes (vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (pcr) to de ... | 2013 | 24589112 |
| development of a real-time pcr assay for detection and quantification of anaplasma ovis infection. | anaplasma ovis is a tick-borne intra-erythrocytic rickettsial pathogen of small ruminants. real-time pcr possesses merits of rapidity, accuracy, reliability, automation and ease of standardization, but has not been used for detection of a. ovis, to the best of our knowledge. in this study, a real-time pcr assay was developed for detection and quantification of a. ovis. species-specific primers and taqman probe were designed based on the glta gene. no cross-reactions were observed with anaplasma ... | 2013 | 24589111 |
| coinfection of sheep with anaplasma, theileria and babesia species in the kurdistan region, iraq. | infections of small ruminants with anaplasma, theileria and babesia species are widely distributed in the old world and are of great economic impact. in iraq, data on disease occurrence in sheep caused by above-mentioned infectious agents are scarce. this study provides information on various haemoparasitic agents infecting sheep in the kurdistan region, iraq, using molecular diagnostic tools. altogether, 195 samples originating from three governorates in the kurdistan region, namely duhok, erbi ... | 2013 | 24589110 |
| can anaplasma ovis in small ruminants be neglected any longer? | anaplasma species are obligate intracellular rickettsial pathogens transmitted by ticks with an impact on human and animal health. anaplasma ovis infects sheep and goats in many regions of the world, and it can be diagnosed by different methods like giemsa staining, pcr or competitive elisa. in this study, a pcr based on the gene coding for major surface protein 4 (msp-4) was used to examine field samples collected from sheep in different countries. altogether, 1161 blood samples from turkey (n ... | 2013 | 24589109 |
| a survey of ixodid tick species and molecular identification of tick-borne pathogens. | this study was undertaken in two different climatic areas of turkey to determine the presence of tick-borne pathogens of medical and veterinary importance. the ticks were removed from humans, pooled according to species and developmental stages, and analyzed by pcr, reverse line blot (rlb) and sequencing. of the 2333 removed ticks from 10 species, 1238 (53.06%) were obtained from the arid cold zone, and the remaining 1095 (46.93%) were obtained from the humid zone. the removed ticks were identif ... | 2014 | 24424312 |
| looking for prognosticators in ovine anaplasmosis: discriminant analysis of clinical and haematological parameters in lambs belonging to differently susceptible breeds experimentally infected with anaplasma ovis. | a study was carried out to evaluate the response of different native sheep breeds to experimental infection with anaplasma ovis, the most prevalent sheep tick-borne pathogen in apulia (southern italy). thirty-four lambs belonging to a northern european breed (suffolk) and two southern italian breeds (comisana and altamurana) were infected. eleven clinical as well as haematological parameters were monitored at different temporal resolutions on the same subjects before and after the infection, res ... | 2013 | 24053615 |
| blood parasites of livestock in certain regions in saudi arabia. | blood samples from camels, sheep, goats and cattle from six regions in saudi arabia were examined for blood parasites. asir region camels were disinfected while those of the eastern, jazan, northern frontiers, riyadh and tabouk regions were infected with trypanosoma evansi (5-40%), those of riyadh and the eastern regions were infected with dipetalonema evansi (1-6%) and those of the eastern, jazan and riyadh regions were infected with eperythrozoon species (8-20%). sheep and goats of all tested ... | 0 | 23961044 |
| anaplasma infections in ticks and reservoir host from slovakia. | anaplasma phagocytophilum is a worldwide distributed bacterium with a significant medical and veterinary importance. it grows within the phagosome of infected neutrophils and is responsible for human granulocytic anaplasmosis (hga), tick-borne fever (tbf) of small ruminants and cattle, canine and equine granulocytic anaplasmosis, but infects also a great variety of wildlife species. wild ungulates and rodents are considered reservoirs of infection in natural foci. the objective of this study was ... | 2014 | 23770268 |
| survey of anaplasmataceae bacteria in sheep from senegal. | the authors studied the role of bacteria belonging to anaplasmataceae family as the causes of acute illnesses of sheep in west africa. | 2013 | 23553260 |
| hematologic and clinical aspects of experimental ovine anaplasmosis caused by anaplasma ovis in iran. | anaplasma ovis infections can cause clinical symptoms in acute phase and lead to huge economic losses in flocks. the aim of the present study was to investigate the hematological and parasitological changes in experimental anaplasmosis in sheep with iranian strain of a. ovis. | 2012 | 23323097 |
| development and validation of two pcr tests for the detection of and differentiation between anaplasma ovis and anaplasma marginale. | anaplasma ovis and anaplasma marginale are tick-transmitted bacteria that cause anaplasmosis in domestic and wild animals. recent results show that some domestic and wild animals and ticks are susceptible to both a. ovis and a. marginale, thus supporting the need to differentiate between these species in hosts and ticks diagnosed with anaplasma infection. however, although anaplasmosis is one of the most common diseases of grazing animals worldwide, rapid and effective tests are not available fo ... | 2012 | 23182548 |
| analysis of pathogen co-occurrence in host-seeking adult hard ticks from serbia. | past studies in serbia have reported concurrent infections of ixodes ricinus ticks with borrelia burgdorferi sensu lato genospecies, anaplasma phagocytophilum and francisella tularensis. as a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in serbia. five tick species were identified (dermacentor marginatus, d. reticulatus, haemaphysalis punctata, h. concinna and i. ricinus) and analyzed for the presence of seven pathogen ... | 2013 | 22773070 |
| a recently identified ovine babesia in china: serology and sero-epidemiology. | babesia sp. in xinjiang, transmitted by hyalomma, is a large babesia that is infective for small ruminants, but it has almost no pathogenicity in healthy sheep. on the basis of the sequences of the 18s rrna and internal transcribed spacer (its) genes, morphological characteristics, vector tick species and pathogenicity it was identified recently as a novel babesia species. in the present study, an enzyme-linked immunosorbent assay (elisa) was developed using soluble merozoite antigens of babesia ... | 2012 | 22579523 |
| molecular survey and genetic identification of anaplasma species in goats from central and southern china. | anaplasma species are obligate intracellular rickettsial pathogens that impact the health of humans and animals. few studies have been carried out on anaplasma infections in central and southern china. this study was conducted to determine the coinfection rates of anaplasma ovis, a. bovis, and a. phagocytophilum from 262 field blood samples of goats in these regions. the average prevalences of single infection of a. ovis, a. bovis, and a. phagocytophilum were 15.3, 16.0, and 6.1%, respectively. ... | 2012 | 22057867 |
| blood parasites of sheep in the netherlands. i. anaplasma mesaeterum sp.n. (rickettsiales, anaplasmataceae). | summary on two occasions an anaplasm was isolated from sheep on the dutch island of ameland. the organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. treatment with oxytetracycline was effective. its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. this anaplasm differs from anaplasma ovis in that less than 30% ... | 1979 | 22040472 |
| first molecular evidence of anaplasma ovis and rickettsia spp. in keds (diptera: hippoboscidae) of sheep and wild ruminants. | to evaluate the presence of rickettsial agents in hippoboscid flies with molecular methods, 81 sheep keds (melophagus ovinus) were collected from 23 sheep, 144 deer keds (lipoptena cervi) were caught in the environment, and a further 463 and 59 individuals of the latter species were obtained from fresh carcasses of 29 red deer and 17 roe deer, respectively. dna was extracted individually or in pools. anaplasma ovis was demonstrated in all examined sheep keds, and from one pool of free-living dee ... | 2011 | 21923269 |
| development of a loop-mediated isothermal amplification method for detection of theileria lestoquardi. | a loop-mediated isothermal amplification (lamp) assay was developed for the diagnosis of theileria lestoquardi infection. the primers were designed based on the clone-5 sequence of t. lestoquardi. the specificity and sensitivity of the assay were established. analysis of the specificity showed that the selected lamp primers amplified the target sequence from t. lestoquardi dna successfully, while no amplification was seen with dna from theileria annulata, theileria ovis, babesia ovis, anaplasma ... | 2011 | 21744022 |
| emergence and genetic variability of anaplasma species in small ruminants and ticks from central europe. | anaplasmoses are common tick-borne zoonotic bacterial diseases of livestock and free-living ungulates from the genus anaplasma that are recently emerging in central europe. the main aim of this study was to analyze the prevalence and genetic variability of anaplasma phagocytophilum and anaplasma ovis in small ruminants and questing ticks from six different sites in slovakia and the czech republic using the pcr of the msp4 gene followed by the sequence analysis. at two farms from southeastern slo ... | 2011 | 21684091 |
| development and evaluation of a loop-mediated isothermal amplification method for rapid detection of anaplasma ovis. | anaplasma ovis is an intraerythrocytic rickettsial pathogen of small ruminants. loop-mediated isothermal amplification (lamp) is a nucleic acid detection method in which the target dna can be efficiently amplified with high specificity and sensitivity under isothermal conditions. in this study, a lamp method was developed for the specific detection of a. ovis, using lamp primers designed on the basis of the major surface protein 4 gene. lamp was performed at 65°c for 30 min. its specificity was ... | 2011 | 21471346 |
| tick-borne bacteria in mouflons and their ectoparasites in cyprus. | the cypriot mouflon (ovis orientalis ophion), a once almost extirpated species of wild sheep, is under strict surveillance because it can be threatened by likely transmission of pathogenic bacteria, such as anaplasma spp., rickettsia spp., and coxiella burnetii, primarily from domestic ungulates. we collected 77 blood samples from cypriot mouflons and 663 of their ectoparasites (rhipicephalus turanicus, rhipicephalus sanguineus, rhipicephalus bursa, hyalomma anatolicum excavatum, hyalomma margin ... | 2011 | 21441182 |
| treatment and control of chlamydial and rickettsial infections in sheep and goats. | small ruminants are susceptible to several chlamydial and rickettsial infections. some of them, such as ehrlichia ruminantium, have a great impact on the sheep and goat industry while others, such as coxiella burnetii, are important zoonotic agents. this review focuses on measures of treatment and control for the following organisms: chlamydophila abortus (formerly chlamydia psittaci immunotype 1), coxiella burnetii, anaplasma ovis, anaplasma phagocytophilum, and ehrlichia ruminantium. | 2010 | 21215905 |
| fatal bovine anaplasmosis in a herd with new genotypes of anaplasma marginale, anaplasma ovis and concurrent haemoplasmosis. | haematological and molecular analysis of blood samples was carried out during an outbreak of bovine anaplasmosis in hungary. acute disease was observed in five animals, two of which died. anaplasma-carrier state was diagnosed in 69 (92%) of cattle. further evaluation of 24 blood samples revealed concurrent infections with mycoplasma wenyonii and 'candidatusm. haemobos' in 22 and 21 animals, respectively. in addition, two cows were identified with rickettsaemia. regarding molecular investigation ... | 2010 | 21094505 |
| characterization of pathogen-specific expression of host immune response genes in anaplasma and mycobacterium species infected ruminants. | anaplasma and mycobacterium species are among the most prevalent bacterial pathogens in european red deer (cervus elaphus) in south-central spain and are known to modify gene expression in ruminants. in this study, we used microarray hybridization and real-time rt-pcr analyses to characterize global gene expression profiles in red deer in response to anaplasma ovis and a. ovis/mycobacterium bovis/mycobacterium avium sub. paratuberculosis (map) infections, compare the expression of immune respons ... | 2010 | 20952064 |
| survey on blood-sucking lice (phthiraptera: anoplura) of ruminants and pigs with molecular detection of anaplasma and rickettsia spp. | lice may serve as biological or mechanical vectors for various infectious agents. to investigate louse infestation of ruminants and pigs, and pathogens potentially transmitted by them, anopluran lice (n=1182) were collected in hungary, and evaluated for the presence of anaplasma, rickettsia and haemotropic mycoplasma dna. on cattle the following species were found: linognathus vituli (57%), haematopinus eurysternus (38%) and solenopotes capillatus (5%). l. vituli had a lower mean individual coun ... | 2010 | 20943320 |
| application of highly sensitive saturation labeling to the analysis of differential protein expression in infected ticks from limited samples. | abstract: | 2010 | 20704695 |
| human anaplasmosis and anaplasma ovis variant. | 2010 | 20507768 | |
| characterization of anaplasma phagocytophilum and a. ovis infection in a naturally infected sheep flock with poor health condition. | anaplasma species are transmitted by ticks and cause diseases in humans and animals. these pathogens infect sheep, an economically important domestic animal worldwide. the current study was designed to characterize in 200 animals the infection with anaplasma phagocytophilum and anaplasma ovis and the genetic diversity of a. ovis strains collected from a naturally infected sheep flock with poor health condition. sheep had 98% seroprevalence to anaplasma spp. antibodies. pcr results confirmed the ... | 2010 | 20405320 |
| merozoite proteins from babesia sp. bq1 (lintan) as potential antigens for serodiagnosis by elisa. | babesia sp. bq1 (lintan) is a babesia isolated from sheep infested with haemaphysalis qinghaiensis in china, and is closely related to b. motasi based on the 18s rrna gene sequence. in the present study, an elisa was developed with merozoite antigens of babesia sp. bq1 (lintan) (bqma) purified from in vitro culture. when the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95.5%. except for babesia sp. tianzhu, there was no cross- ... | 2010 | 20109252 |
| identification of theileria uilenbergi immunodominant protein for development of an indirect elisa for diagnosis of ovine theileriosis. | theileriosis of small ruminants in the northwest of china is a protozoan disease that restricts the development of the livestock industry. the disease is caused by infection with theileria uilenbergi and theilerialuwenshuni, both of which are transmitted by ixodid heamaphysalis ticks. the development of serological tools as a means of integrated control of the disease is an urgent and important requirement. here we describe the identification and partial recombinant expression of a t.uilenbergi ... | 2010 | 19900458 |
| phylogentic analysis of anaplasma ovis strains isolated from sheep and goats using groel and mps4 genes. | evidence of anaplasma spp. in goats and sheep in cyprus has been demonstrated by previous research. herein, further research was performed for the identification of the exact anaplasma spp. resulting in the identification of anaplasma ovis strains in all samples examined. we used a bioinformatics as well as a molecular approach (study of groel and mps4 genes) in order to verify the validity of the results. all samples depicted the presence of a. ovis regardless of the host (goat or sheep). | 2009 | 19443140 |
| molecular detection and identification of ehrlichia and anaplasma species in ixodid ticks. | a polymerase chain reaction (pcr) assay followed by partial sequencing of the 16s ribosomal rna gene was performed for the presence of ehrlichia and/or anaplasma. a total of 242 ixodid ticks were collected from domestic ruminants and their shelters, as well as humans, and their individual salivary glands were dissected out for dna. from the 242 ticks analyzed, six (2.47%), comprising three hyalomma anatolicum anatolicum, one rhipicephalus bursa, and two rhipicephalus sanguineus, were positive. o ... | 2009 | 19247690 |
| small-scale expressed sequence tag analysis of theileria uilenbergi: identification of a gene family encoding potential antigenic proteins. | recently, theileria sp. (china) has been designated as t. luwenshuni[formerly theileria sp. (china 1)] and t. uilenbergi[formerly theileria sp. (china 2)]. a cdna library of t. uilenbergi merozoites was constructed and subjected to random sequencing. among the obtained sequences were three highly identical cdna clones, indicating a gene family. bioinformatic analyses indicated these genes contain signal peptides and encode potential immunogenic proteins. the presence of tandemly arranged and add ... | 2008 | 19120214 |
| molecular characterization of two different strains of haemotropic mycoplasmas from a sheep flock with fatal haemolytic anaemia and concomitant anaplasma ovis infection. | after the first outbreak of fatal mycoplasma ovis infection (eperythrozoonosis) in a sheep flock in hungary (1997), a second wave of the disease was noted in 2006, with different seasonal pattern and affected age group, as well as increased mortality (5.5%). the aim of the present study was to molecularly characterize the causative agent and to reveal underlying factors of the second wave of the disease. remarkably, among the 33 sheep examined, 17 were infected with two strains of haemotropic my ... | 2009 | 19091491 |
| prevalence and genotypes of anaplasma species and habitat suitability for ticks in a mediterranean ecosystem. | anaplasma species are tick-transmitted pathogens that impact veterinary and human health. sicily is one of the locations where these pathogens are endemic. sicily represents a typical mediterranean ecosystem to study anaplasma infection and tick habitat suitability. the aims of this study were (i) to characterize by 16s rrna and species-specific msp4 gene pcr the prevalence and genotypes of a. marginale, a. phagocytophilum, and a. ovis in the most abundant host species in sicilian provinces and ... | 2008 | 18978093 |
| a novel clinical syndrome and detection of anaplasma ovis in mongolian reindeer (rangifer tarandus). | the tsaatan (or dhuka) peoples of northern-western mongolia are one of the few remaining reindeer-herding cultural groups in the world. recently a disease condition that involves sudden death of reindeer and cases involving fever, lethargy, and pale mucous membranes has been reported. examination of blood smears collected in the 2005 field season resulted in the identification of intra-erythrocytic inclusions resembling anaplasma spp. in smears from clinically sick animals. using universal polym ... | 2008 | 18689641 |
| ticks of small ruminants in china. | the importance of ticks and tick-borne diseases of small ruminants in china is discussed. of the 109 species of ticks identified to date in china, 45 species infest small ruminants. five species have been proved to be involved, or possibly involved, in the transmission of tick-borne diseases. anaplasma ovis, babesia motasi, babesia ovis and two unidentified species of theileria, have been recorded in small ruminants in china. the diseases caused by these organisms are widespread in china, causin ... | 2007 | 17823826 |
| molecular detection of ehrlichia ruminantium infection in amblyomma variegatum ticks in the gambia. | in west africa, losses due to heartwater disease are not known because the incidence/prevalence has not been well studied or documented. to develop a diagnostic tool for molecular epidemiology, three pcr-based diagnostic assays, a nested pcs20 pcr, a nested map1 pcr and a nested reverse line blot (rlb) hybridization assay, were evaluated to determine their ability to detect infection in vector ticks, by applying them simultaneously to a. variegatum field ticks to detect ehrlichia ruminantium, th ... | 2007 | 17476576 |
| detection and quantification of anaplasma marginale dna in blood samples of cattle by real-time pcr. | a taqman-based real-time pcr assay was developed for the diagnosis of anaplasma marginale infection of cattle. the established assay was proven to be highly specific, since no cross-reactions were observed with other anaplasma species of ruminants, including the closely related anaplasma centrale, or other haemoparasites of ruminants (anaplasma bovis, anaplasma ovis, anaplasma phagocytophilum, babesia bovis, babesia bigemina, theileria annulata and theileria buffeli). the detection limit was equ ... | 2007 | 17466470 |
| first serological and molecular evidence on the endemicity of anaplasma ovis and a. marginale in hungary. | recurring and spontaneously curing spring haemoglobinuria was recently reported in a small sheep flock in a selenium deficient area of northern hungary. in blood smears of two animals showing clinical signs, anaplasma-like inclusion bodies were seen in erythrocytes. to extend the scope of the study, 156 sheep from 5 flocks and 26 cattle from 9 farms in the region were examined serologically with a competitive elisa to detect antibodies to anaplasma marginale, a. centrale and a. ovis. the seropos ... | 2007 | 17336001 |
| an epidemiological study on anaplasma infection in cattle, sheep, and goats in mashhad suburb, khorasan province, iran. | the prevalence of anaplasma infection was studied in cattle, sheep, and goats in the mashhad area from 1999 to 2002. a total of 160 cattle from 32 farms and 391 sheep and 385 goats from 77 flocks were clinically examined for the presence of anaplasma spp. in blood smears. the study revealed that 19.37% of cattle were infected with anaplasma marginale and 80.3% of sheep and 38.92% of goats were infected with anaplasma ovis. prevalence of anaplasma infection between male and female and between dif ... | 2006 | 17114758 |
| sequence analysis of the msp4 gene of anaplasma ovis strains. | anaplasma ovis (rickettsiales: anaplasmataceae) is a tick-borne pathogen of sheep, goats and wild ruminants. the genetic diversity of a. ovis strains has not been well characterized due to the lack of sequence information. in this study, we evaluated bighorn sheep (ovis canadensis) and mule deer (odocoileus hemionus) from montana for infection with a. ovis by serology and sequence analysis of the msp4 gene. antibodies to anaplasma spp. were detected in 37% and 39% of bighorn sheep and mule deer ... | 2007 | 17052866 |
| genetic characterization of anaplasma ovis strains from bighorn sheep in montana. | wildlife reservoir species and genetic diversity of anaplasma ovis (rickettsiales: anaplasmataceae) have been poorly characterized. bighorn sheep (ovis canadensis), captured in montana from december 2004 to january 2005, were tested for antibodies to anaplasma spp.; the presence of a. ovis was determined by the characterization of major surface protein msp4 sequences. anaplasma antibodies were detected in 25/180 (14%) sampled bighorn sheep and a. ovis msp4 sequences were amplified by polymerase ... | 2006 | 16870861 |
| differential expression and sequence conservation of the anaplasma marginale msp2 gene superfamily outer membrane proteins. | bacterial pathogens in the genera anaplasma and ehrlichia encode a protein superfamily, pfam01617, which includes the predominant outer membrane proteins (omps) of each species, major surface protein 2 (msp2) and msp3 of anaplasma marginale and anaplasma ovis, anaplasma phagocytophilum msp2 (p44), ehrlichia chaffeensis p28-omp, ehrlichia canis p30, and ehrlichia ruminantium map1, and has been shown to be involved in both antigenic variation within the mammalian host and differential expression b ... | 2006 | 16714578 |
| serologic and molecular characterization of anaplasma species infection in farm animals and ticks from sicily. | although anaplasma marginale was known to be endemic in italy, the diversity of anaplasma spp. from this area have not been characterized. in this study, the prevalence of anaplasma spp. antibodies in randomly selected farm animals collected on the island of sicily was determined by use of a msp5 celisa for anaplasma spp. and an immunofluorescence test specific for anaplasma phagocytophilum. genetic variation among strains of anaplasma spp. from animals and ticks was characterized using the a. m ... | 2005 | 16043300 |
| amplification of 16s rrna genes of anaplasma species in china for phylogenetic analysis. | in this study, a phylogenetic tree was inferred through comparing five 16s rrna gene sequences of four isolates of anaplasma ovis and one of anaplasma marginale in china with all nineteen 16s rrna gene sequences deposited in genbank (12 a. marginale, 3 a. ovis and 4 anaplasma centrale derived from america, uruguay, south africa, zimbabwe, australia, isreal and japan). the analysis showed that all a. ovis isolated in china were separated into an a. ovis cluster, while the a. marginale in china wa ... | 2005 | 15795086 |
| seroprevalence of anaplasmosis among cattle in switzerland in 1998 and 2003: no evidence of an emerging disease. | anaplasma marginale infection in europe has been limited to the mediterranean and eastern countries, to austria and to very sporadic cases in switzerland. there are no reports of its occurrence in the countries north of switzerland. a severe outbreak of anaplasmosis in august 2002 in a cattle farm in the canton grisons, switzerland, north of the alps, with more than 300 cattle that had to be culled, came unexpected and gave reason to hypothesize presence of an increased yet undetected prevalence ... | 2005 | 15795079 |
| anaplasmosis. vii. experimental anaplasma ovis infection in white-tailed deer (dama virginiana). | 1963 | 14035794 | |
| anaplasma ovis in the united states; experimental studies with sheep and goats. | 1956 | 13340112 | |
| identification of borrelia burgdorferi sensu lato, anaplasma and ehrlichia species, and spotted fever group rickettsiae in ticks from southeastern europe. | prevalence data for tick-borne pathogens are used to assess the risk for human health. in this study the presence and identity of borrelia burgdorferi sensu lato, ehrlichia, anaplasma, and rickettsia species in bulgarian ixodes ricinus ticks and in non- ixodes ticks from turkey and albania was determined by polymerase chain reaction (pcr) and reverse line blot hybridization. in the adult bulgarian ticks, the prevalence of borrelia burgdorferi sensu lato infection was approximately 40%, while bor ... | 2003 | 12938010 |
| infection of tick cells and bovine erythrocytes with one genotype of the intracellular ehrlichia anaplasma marginale excludes infection with other genotypes. | anaplasma marginale, a tick-borne rickettsial pathogen of cattle, is endemic in several areas of the united states. many geographic isolates of a. marginale that occur in the united states are characterized by the major surface protein 1a, which varies in sequence and molecular weight due to different numbers of tandem repeats of 28 or 29 amino acids. recent studies (g. h. palmer, f. r. rurangirwa, and t. f. mcelwain, j. clin. microbiol. 39:631-635, 2001) of an a. marginale-infected herd of catt ... | 2002 | 11986275 |
| health protocol for translocation of free-ranging elk. | when considering an elk (cervus elaphus) restoration program, wildlife managers must evaluate the positive and negative elements of translocation. we prepared this protocol to give an overview of health considerations associated with translocation of elk, with an emphasis on movement of free-ranging elk from western north america to the southeastern usa. we evaluated infectious agents and ectoparasites reported in elk from two perspectives. first, we made a qualitative estimate of the ability of ... | 2001 | 11504216 |
| cattle and small ruminant piroplasmosis in macedonia, greece. | macedonia is an endemic region of cattle as well as of sheep and goat piroplasmosis in cattle, theileria orientalis (=t. buffeli?), the agent of eurasian benign theileriosis, is the most common and widespread piroplasm species. t. annulata, the agent of tropical theileriosis, seems to be rare and limited to few foci, but causes very severe clinical cases, especially in imported pure-bred or cross-bred animals. babesia bovis and b. bigemina are present in several localities, and often coexist. ca ... | 1999 | 11071550 |
| dermacentor hunteri (acari: ixodidae): an experimental vector of anaplasma marginale and a. ovis (rickettsiales: anaplasmataceae) to calves and sheep. | the experimental vector competence of laboratory-reared dermacentor hunteri bishopp for anaplasma marginale theiler and anaplasma ovis lestoquard was evaluated by delayed transfer of male ticks from infected to susceptible holstein calves and from infected to susceptible domestic sheep, respectively. after feeding for 4 or 5 d on rickettsemic acquisition hosts, the ticks were held off the host at 26 degrees c, approximately 93% rh, and a photoperiod of 14:10 (l:d) h for 7 or 8 d, then test fed f ... | 1999 | 10337102 |
| persistence of anaplasma ovis infection and conservation of the msp-2 and msp-3 multigene families within the genus anaplasma. | goats which have recovered from acute anaplasma ovis infection remain seropositive, although infected erythrocytes cannot be detected by microscopic examination. persistence of a. ovis 17 to 21 months following experimental infection was demonstrated by pcr detection of the msp-5 gene. quantitative analysis of persistent rickettsemia over time showed that all levels were below the limit of microscopic detection and ranged from a low of 10(2) organisms/ml to peaks of 10(6) organisms/ml. two patte ... | 1998 | 9826393 |
| cd4(+) t-lymphocyte and immunoglobulin g2 responses in calves immunized with anaplasma marginale outer membranes and protected against homologous challenge. | protective immunity against the ehrlichial pathogen anaplasma marginale has been hypothesized to require induction of immunoglobulin g2 (igg2) antibody against outer membrane protein epitopes and coordinated activation of macrophages for phagocytosis and killing. in the present study, cell-mediated immune responses, including induction of igg isotype switching, were characterized in calves immunized with purified outer membranes of the florida strain of a. marginale. importantly, these calves we ... | 1998 | 9784551 |
| status of the tick-borne diseases in sheep and goats in turkey. | a serological survey using indirect fluorescent antibody test (ifat) for babesia ovis infection of sheep has been carried out in different geographical regions of turkey. the results indicated that 71.6% of 141 sheep in black sea region, 70.9% of 93 sheep in central anatolia, 80.2% of 96 sheep in aegean region and 55.7% of 122 sheep in eastern anatolia were seropositive. this means that b. ovis infection is endemic throughout turkey. in addition, occurrence of b. ovis, b. motasi, theileria hirci ... | 1997 | 9530701 |
| tick-borne diseases of sheep and goats and their related vectors in iran. | haemoparasitic diseases have long been considered as a major problem to efficient sheep and goats production in iran. theileriosis due to theileria hirci and babesiosis due to babesia ovis and b. motasi are the most pathogenic protozoa. b. crassa, anaplasma ovis and eperythrozoon ovis are usually non-pathogenic and do not cause any apparent problem. the major tick genera found on sheep and goats are hyalomma, rhipicephalus, haemaphysalis, ixodes and rarely dermacentor distributed in all part of ... | 1997 | 9530694 |
| tick-borne diseases of sheep and goats caused by babesia, theileria or anaplasma spp. | a review is given on the babesia, theileria, and anaplasma species infecting sheep and goats. b. ovis is the most important disease agent. it is transmitted by rhipicephalus bursa, r. turanicus, hyalomma anatolicum excavatum, and probably by r. evertsi evertsi b. ovis is widely spread in southern europe, the middle east, and central asia. its geographical distribution in south and east asia and in africa is widely unknown. b. motasi obviously represents several nosodemes in separate regions. it ... | 1997 | 9530692 |
| piroplasms of domestic animals in the macedonia region of greece. 3. piroplasms of small ruminants. | a study was carried out on piroplasms of small ruminants in the macedonia region of greece. during 1984-1985, 721 serum samples were collected from sheep in 49 localities and 487 from goats in 43 localities. blood smears were also prepared from 26 sheep and eight goats in 16 localities. the prevalence of positive ifa titres for sheep and goat sera, respectively, was: 24.6% and 0.6% for theileria ovis, 52.1% and 36.4% for babesia ovis, 10.5% and 4.2% for babesia motasi, 12.6% and 6.6% for babesia ... | 1996 | 8792581 |
| evaluation of anaplasma marginale major surface protein 3 (msp3) as a diagnostic test antigen. | an immunodominant surface protein, major surface protein 3 (msp3), has been proposed as an antigen suitable for use in the diagnosis of bovine anaplasmosis. we further characterized msp3 to examine its potential as a test antigen for the serological diagnosis of carrier cattle. the specificity of this antigen in detecting infected cattle as well as the conservation of msp3 between strains of anaplasma marginale was evaluated by using immunoblots of a. marginale proteins separated by one- and two ... | 1996 | 8788999 |
| susceptibility of elk (cervus elaphus) to experimental infection with anaplasma marginale and a. ovis. | anaplasma ovis was experimentally transmitted from domestic sheep to elk (cervus elaphus) and back to splenectomized sheep. no rickettsemias were detected but serum from three of seven experimentally inoculated elk developed anaplasma spp.-reactive antibody as measured by indirect immunofluorescence (iif) or by the rapid card agglutination and complement fixation assays. three elk were experimentally infected with a. marginale. the rickettsiae were detected in blood of these elk and caused disea ... | 1996 | 8627938 |
| antigen profiles of anaplasma ovis and a. mesaeterum and cross infection trials with them and a. marginale. | antigen profiles of anaplasma ovis and a. mesaeterum were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, and cross infection trials were performed by a. ovis challenge to a. mesaeterum- or a. marginale-inoculated goats and a. marginale challenge to an a. ovis-inoculated calf. antigen analysis showed unique and common proteins of a. ovis and a. mesaeterum and identified a major 38 kda protein to be a dominant immunogen bearing two epitopes common to anap ... | 1993 | 8296449 |
| a retrospective serologic survey for anaplasma spp. infection in three bighorn sheep (ovis canadensis) populations in california. | using an indirect immunofluorescence assay, we determined the prevalence of anaplasma-reactive antibody in three herds of bighorn sheep, each a different subspecies and occupying a different habitat in california (usa). antibodies to anaplasma spp. were identified in none of twenty california bighorn (ovis canadensis californiana) sampled from the mt. baxter herd, 11 of 17 peninsular bighorn (o. canadensis cremnobates) sampled in the santa rosa mountains, and all 20 desert bighorn (o. canadensis ... | 1993 | 8258852 |
| characterization of an anaplasma ovis isolate from desert bighorn sheep in southern california. | to understand the role of bighorn sheep (ovis canadensis) in the epidemiology of anaplasmosis, we recovered a field isolate from a suspected enzootic area in southern california (usa). whole blood was collected from three desert bighorn sheep (ovis canadensis nelsoni) and inoculated into a susceptible splenectomized domestic sheep, calf and a susceptible spleen-intact bighorn sheep. no infection occurred in the calf, but a detectable infection developed in both the domestic sheep and bighorn she ... | 1993 | 8258851 |
| [ticks and hemoparasitoses in livestock in senegal. v. the northern guinea area]. | the authors report the results of a study on ticks and hemoparasitoses among cattle, sheep and goats in the north-guinean zone. during a period of 15 months, ticks were systematically removed from 40 cattle, 40 sheep and 40 goats in order to assess the population dynamics and to determine more accurately the preferential sites of settlement of the following different species collected from these animals: amblyomma variegatum, boophilus geigyi, hyalomma truncatum, h. marginatum rufipes, rhipiceph ... | 1993 | 8073170 |
| [ticks and hemoparasitoses of livestock in senegal. vi. the soudano-sahelian zone]. | the authors report the results of a study on ticks and hemoparasitoses among cattle, sheep and goats in the sudano-sahelian zone. during a period of 15 months, ticks were systematically removed from 40 cattle, 40 sheep and 40 goats in order to assess the population dynamics and determine more accurately the preferential sites of settlement of the different species. the following species were collected in ruminants: hyalomma truncatum, h. marginatum rufipes, rhipicephalus evertsi evertsi, rh. gui ... | 1994 | 7991897 |
| detection of anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay. | a competitive inhibition enzyme-linked immunosorbent assay (elisa) based on a major surface protein 5 (msp5) b-cell epitope conserved among anaplasma species was used to detect goats infected with anaplasma ovis. we examined strains of a. ovis isolated from goats in kenya and demonstrated that msp5 and the target b-cell epitope, bound by monoclonal antibody anaf16c1, were conserved. sera from 149 goats in four regions of kenya and from 302 goats in six u.s. states were tested for the presence of ... | 1995 | 7538510 |
| infection of splenectomized calves with anaplasma ovis. | an anaplasma was not recovered from 2 splenectomized calves at 17 days after a ovis inoculation, but was recovered from 1 of the calves at 177 days after inoculation. in a 3rd calf exposed to a ovis, an anaplasma was recovered at 177 and 262 days after inoculation. the anaplasma recovered from these calves was determined to be a ovis on the basis of cross-infectivity trials, using anaplasma marginale. | 1981 | 7340580 |