Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| analysis of the effect of cyclophosphamide and methotrexate on chrysomya megacephala (diptera: calliphoridae). | forensic entomotoxicology investigates the effects of chemical substances in the development of scavenger insects and the reflection on estimating the minimum postmortem interval (pmi). to evaluate the impact of the chemotherapeutic drugs cyclophosphamide (cf) and methotrexate (mtx) on the postembryonic development of chrysomya megacephala, controlled experiments were performed by rearing the larvae on spiked minced beef with different concentrations of these drugs. the results indicated that cf ... | 2018 | 29351362 |
| ultrastructure of male genitalia of blow flies (diptera: calliphoridae) of forensic importance. | male genitalia of blow flies (diptera: calliphoridae) are distinctive in their morphological features and are often used for species identification. the aim of this work was to investigate the male genitalia of blow flies of medical and forensic importance from thailand at the ultrastructural level, using scanning electron microscopy (sem). flies in two subfamilies were examined: chrysomyinae [chrysomya bezziana villeneuve, chrysomya chani kurahashi, chrysomya nigripes aubertin, chrysomya pingui ... | 2017 | 29248415 |
| can escherichia coli fly? the role of flies as transmitters of e. coli to food in an urban slum in bangladesh. | to investigate the transmission of faecal bacteria by flies to food under natural settings. | 2018 | 29121443 |
| longevity, fertility and fecundity of adult blow flies (diptera: calliphoridae) held at varying densities: implications for use in bioconversion of waste. | large numbers of flies are needed to produce the quantity of larvae required for insect bioconversion of waste. however, this 'mass-rearing' may negatively affect adult survival and reproductive output. this study assessed the suitability for mass-rearing of four blow fly species, chrysomya chloropyga, chrysomya chloropyga (wiedemann), chrysomya megacephala (f.), chrysomya putoria (wiedemann) and lucilia sericata (meigen). flies were kept at densities of 20, 50, 100, 250, 500, and 1,000 flies pe ... | 2017 | 29040631 |
| breeding of chrysomyia megacephala in closed septic tanks. | 1943 | 29012118 | |
| quantifying pteridines in the heads of blow flies (diptera: calliphoridae): application for forensic entomology. | in forensic cases involving entomological evidence, establishing the postcolonization interval (post-ci) is a critical component of the investigation. traditional methods of estimating the post-ci rely on estimating the age of immature blow flies (diptera: calliphoridae) collected from remains. however, in cases of delayed discovery (e.g., when remains are located indoors), these insects may have completed their development and be present in the environment as adults. adult fly collections are o ... | 2017 | 28942377 |
| insect succession on pig carcasses using different exposure time - a preliminary study in guangzhou, china. | the exposure time of carcasses in insect succession studies are generally not consistent and more than 40% studies start in the morning. how such an arrangement affects the succession of insects is worth evaluating. in this study, six piglet carcasses (15-17 kg) were exposed at 6 time points during the day: 11:00, 15:00, 19:00, 23:00, 03:00 and 07:00 on july 20th-july 21st 2006 in guangzhou, china to investigate the potential effects of the time of day of carcass exposure on body decomposition a ... | 2017 | 28843096 |
| field evaluation of a semi-automatic funnel trap targeted the medically important non-biting flies. | bait-trapping is a useful approach for monitoring fly population dynamics, and it is an effective tool for physical control of pest species. the aim of this study was to test a newly developed semi-automatic funnel fly trap with some modifications of the former prototype fly trap to study medically important fly population density. the efficacy of the semi-automatic funnel trap was assessed by field sampling during july 2013-june 2014 using 1-day tainted beef offal as bait. the modified semi-aut ... | 2017 | 28743450 |
| forensic entomotoxicology revisited-towards professional standardisation of study designs. | forensic entomotoxicology is the use of insects as evidence of whether a toxicant is present in an environment such as a corpse, river or landscape. the earliest overtly forensic study was published in 1977, and since then, at least 63 papers have been published, most of them focused on the detection of toxicants in insects or on effects of toxicants on diverse insect indicator taxa. a comprehensive review of the published literature revealed various inconsistencies between studies that could be ... | 2017 | 28567525 |
| variation in the time of colonization of broiler carcasses by carrion flies in nakhonsawan province, thailand. | carrion flies are the primary insects colonizing vertebrate carrion; however, limited information is available on the variation in the time of colonization (toc) as related to time of placement (top) and time of death (tod), particularly in thailand. three seasonal sets of nine broiler carcasses (euthanized and placed in field within 0.5 h after death) were placed in mesh enclosures within a disturbed deciduous dipterocarp forest at nakhonsawan province, upper-central thailand, for 3 d to determ ... | 2017 | 28444209 |
| laboratory evaluation of brevibacillus laterosporus strains as biocidal agents against chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) larvae. | the biocidal activity of three strains of brevibacillus laterosporus upon the post-embryonic developmental stages of chrysomya megacephala was evaluated. bioassays were performed to verify lethal and sub-lethal effects including ultra-structural changes in the midgut. among the strains assayed, shi3 presented the highest larval mortality rates, achieving 70% at a concentration of 1×10(8) spores/g of diet. transmission electron microscopy revealed intestinal alterations caused by all strains test ... | 2017 | 28442400 |
| dna-based identification of forensically important blow flies (diptera: calliphoridae) from india. | correct species identification is the first and the most important criteria in entomological evidence-based postmortem interval (pmi) estimation. although morphological keys are available for species identification of adult blow flies, keys for immature stages are either lacking or are incomplete. in this study, cytochrome oxidase subunit 1 (coi) reference data were developed from nine species (belonging to three subfamilies, namely, calliphorinae, luciliinae, and chrysomyinae) of blow flies fro ... | 2017 | 28419283 |
| nocturnal oviposition behavior of blowflies (diptera: calliphoridae) in the southern hemisphere (south africa and australia) and its forensic implications. | published research has offered contradictory evidence of the occurrence of nocturnal oviposition by carrion-breeding blowflies, a behavior that can affect the interpretation of forensic estimates of a minimum post mortem interval (minpmi) by up to 12 hours, depending on latitude and season. the majority of published studies are from the northern hemisphere. field experiments were conducted in south africa and australia that extend observations to species of the southern hemisphere. various verte ... | 2017 | 28409386 |
| diversity of sarcosaprophagous calyptratae (diptera) on sandy beaches exposed to increasing levels of urbanization in brazil. | sandy beaches are among the most impacted ecosystems worldwide, and the effects of urbanization on the biodiversity of these habitats are largely unknown, particularly in brazil. we investigated the composition and structure of assemblages of sarcosaprophagous insects (diptera: calliphoridae, sarcophagidae, and muscidae) on six sandy beaches exposed to differential levels of human impact in pernambuco state, brazil. in total, 20,672 adults of 40 species were collected, of which 70% were callipho ... | 2017 | 28369231 |
| sterilization of blow fly eggs, chrysomya megacephala and lucilia cuprina, (diptera: calliphoridae) for maggot debridement therapy application. | maggot debridement therapy (mdt) is an application of sterile laboratory-reared blow fly larvae to remove necrotic tissue and disinfect wounds for medical conditions. for effective application, the blow fly larvae used in the wound treatment are required to be in aseptic condition. here, we report the results of a detailed assessment of bacteria and fungi isolated from the eggs of two blow fly species, chrysomya megacephala (f.) and lucilia cuprina (wiedemann) before and after sterilization by d ... | 2017 | 28361272 |
| wohlfahrtiimonas chitiniclastica: current insights into an emerging human pathogen. | since the first description of wohlfahrtiimonas chitiniclastica in 2008, a number of well described case reports demonstrating its pathogenic role in humans have been published. infections may be closely linked to flies, such as wohlfahrtia magnifica, lucilia sericata, chrysomya megacephala or musca domestica. these insects are potent vectors for the distribution of w. chitiniclastica causing local or systemic infections originating from wounds infested with fly larvae. however, other potential ... | 2017 | 28162132 |
| volume of larvae is the most important single predictor of mass temperatures in the forensically important calliphorid, chrysomya megacephala (diptera: calliphoridae). | calliphorid species form larval aggregations that are capable of generating heat above ambient temperature. we wanted to determine the relationship between volume, number of larvae, and different combinations of instars on larval mass heat generation. we compared different numbers of chrysomya megacephala (f.) larvae (40, 100, 250, 600, and 2,000), and different combinations of instars (∼50/50 first and second instars, 100% second instars, ∼50/50 second and third instars, and 100% third instars) ... | 2017 | 28082629 |
| chemotaxonomic profile and intraspecific variation in the blow fly of forensic interest chrysomya megacephala (diptera: calliphoridae). | necrophagous insects such as blow flies (diptera: calliphoridae) are considered crucial in forensic entomology. identification at species level and determination of larval stage are the basis for estimation of postmortem interval (pmi). insect evidence can also be used in the determination of crime scenes, since body displacement is common. the aim of this study was to determine the chemotaxonomic profile and intraspecific variability of the forensically important blow fly chrysomya megacephala ... | 2017 | 28082627 |
| species-level identification of the blowfly chrysomya megacephala and other diptera in china by dna barcoding. | the blowfly chrysomya megacephala, or oriental latrine fly, is the most common human-associated fly of the oriental and australasian regions. chrysomya megacephala is of particular interest for its use in forensic entomology and because it is a disease vector. the larvae are economically important as feed for livestock and in traditional chinese medicine. identification of adults is straightforward, but larvae and fragments of adults are difficult to identify. we collected c. megacephala, its al ... | 2017 | 28044459 |
| isolation of the male-specific transformer exon as a method for immature specimen sex identification in chrysomya megacephala (diptera: calliphoridae). | being able to efficiently differentiate between male and female individuals in the immature forms of insects allows for investigations into sexually dimorphic patterns of growth rates and gene expression. for species lacking sex-specific morphological characteristics during these periods, alternative methods must be devised. commonly, isolation of sex determination genes reveals sex-specific band patterns and allows for markers that can be used in insect control. for blow flies, a family that in ... | 2017 | 28031348 |
| comparative fly species composition on indoor and outdoor forensic cases in malaysia. | forensic entomology refers to the science of collection and analysis of insect evidence in order to determine the minimum time period since death. this study aimed to investigate the occurrence of forensically important flies on 34 human remains referred to universiti kebangsaan malaysia medical centre over a period of three years. entomological specimens were collected at the death scenes and/or during autopsies. live specimens were reared into adults while preserved specimens were processed fo ... | 2017 | 27997861 |
| first report of oriental latrine flies causing vaginal myiasis in human. | the present paper reports a case of human vaginal myiasis in a 22 year old woman. this is the first report of this fly species to cause vaginal myiasis in human till now. the infested maggots were removed from the vagina and cultured in laboratory. entomological studies on the emerged flies showed that the infested larvae were oriental latrine fly chrysomya megacephala fabricius. clinical presentation and treatment strategies are discussed also. | 2016 | 27876924 |
| a preliminary study of insect succession in al-ahsaa oasis, in the eastern region of the kingdom of saudi arabia. | rabbit carcass decomposition was examined in four seasons in al-ahsaa oasis. decomposition rate was significantly faster in summer compared with other seasons. fourteen insect species from four orders and seven families were recorded: calliphoridae, muscidae, sarcophagidae, formicidae, histeridae, dermestidae, and tenebrionidae. most of the fly maggots collected were calliphoridae with only one species from both muscidae and sarcophagidae. maggots of calliphora vicina (r.-d.) and chrysomya albic ... | 2017 | 27861898 |
| development of the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae), at five constant temperatures. | chrysomya megacephala (fabricius) is a forensically important fly that is found throughout the tropics and subtropics. we calculated the accumulated development time and transition points for each life stage from eclosion to adult emergence at five constant temperatures: 15, 20, 25, 30, and 35 °c. for each transition, the 10th, 50th, and 90th percentiles were calculated with a logistic linear model. the mean transition times and % survivorship were determined directly from the raw laboratory dat ... | 2017 | 27816915 |
| evaluating the effects of different vegetation types on necrophagous fly communities (diptera: calliphoridae; sarcophagidae): implications for conservation. | the present study was conducted in five different phytogeographic zones of the brazilian state of maranhão, three of which (the amazon forest, cerrado, and palm groves) are more heterogeneous, whereas the other two (marshlands and mangroves) are more homogeneous. in each zone, nine sites were visited for the collection of necrophagous flies using bait traps in 2010, 2011, and 2012. the calliphorid and sarcophagid communities observed at each site were compared in terms of species richness, compo ... | 2016 | 27798664 |
| developmental times of chrysomya megacephala (fabricius) (diptera: calliphoridae) at constant temperatures and applications in forensic entomology. | the characteristic life stages of infesting blowflies (calliphoridae) such as chrysomya megacephala (fabricius) are powerful evidence for estimating the death time of a corpse, but an established reference of developmental times for local blowfly species is required. we determined the developmental rates of c. megacephala from southwest china at seven constant temperatures (16-34°c). isomegalen and isomorphen diagrams were constructed based on the larval length and time for each developmental ev ... | 2016 | 27581209 |
| dataset of traumatic myiasis observed for three dominant screw worm species in north west pakistan with first report of wohlfahrtia magnifica (schiner). | regional surveys were carried out in different parts of north west pakistan among domestic animals (n=57,921) including pets and livestock identifying cases of traumatic myiasis (n=1037). a total of four surveys focused general livestock population during eid ul adha (eid surveys; incidence=1.21%) while another four surveys (miscellaneous surveys; incidence=7.34%) targeted animal population brought to veterinary hospitals and dispensaries. timeframe spanned four years from 2012 to 2015. maggots ... | 2016 | 27570812 |
| volume of larvae is the most important single predictor of mass temperatures in the forensically important calliphorid, chrysomya megacephala (diptera: calliphoridae). | calliphorid species form larval aggregations that are capable of generating heat above ambient temperature. we wanted to determine the relationship between volume, number of larvae, and different combinations of instars on larval mass heat generation. we compared different numbers of chrysomya megacephala (f.) larvae (40, 100, 250, 600, and 2,000), and different combinations of instars (∼50/50 first and second instars, 100% second instars, ∼50/50 second and third instars, and 100% third instars) ... | 2016 | 27550079 |
| the classic episode of biological invasion: cochliomyia macellaria (fabricius, 1775) versus chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) - evaluation of the biotic potential. | the classic episode of biological invasion: cochliomyia macellaria (fabricius, 1775) versus chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) - evaluation of the biotic potential. the biotic of the native fly cochliomyia macellaria and the exotic chrysomya megacephala was compared, through the reproductive capacity and longevity. the experiment took place in a climatized chamber, with four repetitions of 15 grouped couples of each species. the posture, egg mass weight, and death o ... | 2016 | 27508990 |
| the forensically important blow fly, chrysomya megacephala (diptera: calliphoridae), is more likely to walk than fly to carrion at low light levels. | one factor that influences estimates of time since death using entomological evidence is whether or not blow flies nocturnally oviposit. field studies focusing on egg laying have found it occurs on an inconsistent basis. a key but poorly understood factor in nocturnal oviposition is a blow fly's ability to locate carrion under low light levels. it has been speculated that blow flies are more likely to walk than fly to carrion during the night, but this has not been empirically tested. we directl ... | 2016 | 27343754 |
| seasonal, locality, and habitat variation in assemblages of carrion-associated diptera in gauteng province, south africa. | seasonal, spatial, and habitat responses of carrion-associated diptera assemblages can provide valuable information about the presence or absence of species and their relative abundance, and thereby enhance understanding of their responses to environmental variables and how this may have an impact on forensic investigations. three different nature reserves (localities) within the municipality of tshwane, south africa, were selected to determine whether species assemblages of carrion-feeding flie ... | 2016 | 27330098 |
| chrysomya megacephala (fabricius) (diptera: calliphoridae) oviposition behavior in previous oviposition situation. | blowflies use their sense of smell to locate oviposition substrates and their vision to direct and assist in landing decision. blowflies' ovipositions are usually aggregated. chrysomya megacephala (fabricius) females, for example, prefer to lay eggs on substrates containing fresh eggs of the same species. however, it is possible that females would be capable of evaluating the substrate, reducing the size of egg cluster in sites with a high amount of eggs or larvae, or finding another site for la ... | 2016 | 27295050 |
| effect of lunar phases, tides, and wind speed on the abundance of diptera calliphoridae in a mangrove swamp. | abiotic factors, such as lunar phases and tides, have a significant effect on insect development. reproduction and immature development are usually interlinked to these abiotic factors. the tide is at its highest levels at full moon or new moon, hindering the feeding of the immature or causing their drowning. the oviposition by adult females is also compromised on these days because much of the available food is submerged. another important abiotic factor is the wind, which displaces odoriferous ... | 2014 | 27193403 |
| larval distribution and behavior of chrysomya rufifacies (macquart) (diptera: calliphoridae) relative to other species on florida black bear (carnivora: ursidae) decomposing carcasses. | larval interactions of dipteran species, blow flies in particular, were observed and documented daily over time and location on five black bear carcasses in gainesville, fl, usa, from june 2002 - september 2004. cochliomyia macellaria (fabricius) or chrysomya megacephala (fabricius) larvae were collected first, after which chrysomya rufifacies (macquart) oviposited on the carcasses in multiple locations (i.e., neck, anus, and exposed flesh) not inhabited already by the other blow fly larvae. wit ... | 2014 | 27193400 |
| the effect of flunitrazepam (rohypnol(®) ) on the development of chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) and its implications for forensic entomology. | this study investigated the potential effects of flunitrazepam (known as "date rape drug") on the developmental cycle of chrysomya megacephala, an important forensic species, and their possible implications for the calculation of the pmi. a 1050 c. megacephala eggs were divided into five groups with seven replications each. the eggs were placed on artificial diet prepared with four drug concentrations of flunitrazepam (4, 8, 16, and 32 ng/g), besides the control group (prepared with water). were ... | 2016 | 27143233 |
| assessment of the abundance and diversity of calliphoridae and sarcophagidae (diptera) in sites with different degrees of human impact in the iberá wetlands (argentina). | the aim of the present study was to explore the diversity and describe the spatial distribution of calliphoridae and sarcophagidae along an urbanization gradient in the iberá wetlands. the flies were collected at 18 sampling points, three per site. in total, 3,318 flies were collected (3,077 blow flies and 241 flesh flies), distributed in 13 genera and 33 species. calliphoridae was the most abundant, comprised 92.74% of all adult flies. chrysomya albiceps (wiedemann), cochliomyia macellaria (f.) ... | 2016 | 27106933 |
| assemblage of necrophagous diptera in atlantic insular environments and response to different levels of human presence. | islands act as natural laboratories for ecological studies to explain bioinvasion processes and, in this scenario, necrophagous diptera have never been used as model organisms. this study aimed to (i) describe assemblages of necrophagous diptera (calliphoridae and sarcophagidae) in two insular environments of different origins and distances from mainland, (ii) investigate the effect of anthropogenic impact on the assemblage of carrion flies, (iii) to quantify the establishment of invasive specie ... | 2016 | 27040531 |
| pandora bullata (entomophthoromycota: entomophthorales) affecting calliphorid flies in central brazil. | fungi are where one finds them, and if one seeks fungal pathogens affecting flies, then a garbage dump may be an ideal place to find both persistent, abundant fly populations and their fungal pathogens. an obvious fungal epizootic affecting the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae), was observed over several days in mid-february 2015 at the local garbage dump adjacent to the city of cavalcante, northern goiás. this site harbored large populations of both c. megacep ... | 2016 | 26968351 |
| large-scale mitogenomics enables insights into schizophora (diptera) radiation and population diversity. | true flies are insects of the order diptera and encompass one of the most diverse groups of animals on earth. within dipterans, schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. based on the nov ... | 2016 | 26912394 |
| intraguild predation influences oviposition behavior of blow flies (diptera: calliphoridae). | the objective of the present study was to determine whether blow flies (diptera: calliphoridae) are able to identify larvae of an intraguild predator species in the substrate and avoid laying eggs there. blow flies oviposited in traps with different treatments: substrate only and substrate with larvae of chrysomya albiceps (wiedemann, 1819), chrysomya megacephala (fabricius, 1794), or chrysomya putoria (wiedemann, 1830). ch. megacephala, ch. putoria, and lucilia eximia (wiedemann, 1819) avoided ... | 2016 | 26888288 |
| antibacterial activity of excretions-secretions from chrysomya megacephala against escherichia coli. | the blowfly, chrysomya megacephala, is distributed worldwide. previous studies found maggot excretions-secretions from other blowfly species inhibited pro-inflammatory response and antimicrobial activity. | 2016 | 26817232 |
| patterns of oviposition and development of chrysomya megacephala (fabricius) (diptera: calliphoridae) and chrysomya rufifacies (macquart) (diptera: calliphoridae) on burned rabbit carcasses. | considering that crimes against animals such as illegal killing and cruelty have been alarmingly increasing and since burning is one of the common ways for disposing cadavers, ability to estimate minimum postmortem interval (pmi) using entomological data merits consideration. chrysomya megacephala and chrysomya rufifacies are common necrophagous species recovered from cadavers in many countries including malaysia. specific studies focusing on the oviposition and developmental patterns of both sp ... | 2016 | 26779962 |
| multiple species of scuttle flies (diptera: phoridae) as contaminants in forensic entomology laboratory insect colony. | in forensic entomology, larval rearing usually includes the presence of biological contaminants including scuttle flies (diptera: phoridae). scuttle flies are recognized as forensically important insects and have been reported causing nuisance and contamination in laboratory environments. this paper reports for the first time the finding of multiple scuttle fly species affecting colonies of third instar larvae of the oriental latrine blowfly, chrysomya megacephala (fabricius) (diptera: calliphor ... | 2015 | 26695221 |
| forensic entomology of high-rise buildings in malaysia: three case reports. | the distributions of flies are not only confined to ground level but can also be at higher altitudes. here, we report three forensic cases involving dipterans in high-rise buildings in kuala lumpur, malaysia. case 1 involved a corpse of adult female found at the top floor of a fifteen-story apartment. case 2 dealt with a body of a 75-year-old female discovered in a bedroom on the eleventh floor of an eighteen-story building, while case 3 was a 52-year-old male found in his fifth floor shop house ... | 2015 | 26691258 |
| edge effects on the blowfly fauna (diptera, calliphoridae) of the tijuca national park, rio de janeiro, brazil. | in this contribution we examine the diversity, abundance and species richness of calliphoridae in the tijuca national park, rio de janeiro, rj. specimens were collected monthly between september 2009 and august 2010, using sardines as bait, in three points: a, on the forest edge (22°56'846"s 43°17'496"w), b, 700 m from it (22°57'073"s 43°17'832"w) and c, 1,200 m into the forest (22°57'321"s 43°18'031"w), evaluating the indicator species at each point and the anthropogenic influences and abiotic ... | 2015 | 26675918 |
| decomposition of concealed and exposed porcine remains in the north carolina piedmont. | we examined the decomposition and subsequent insect colonization of small pig carrion (sus scrofa (l.)) placed in concealed and open environments during spring, summer, and fall in raleigh, north carolina, as a model for juvenile human remains. remains were concealed in simulated attics in three manners, ranging from minimal to well-concealed. concealment had a significant effect on the insect community colonizing the remains across all three seasons; the beetles necrobia rufipes (degeer) (cleri ... | 2016 | 26590193 |
| edge effects on the blowfly fauna (diptera, calliphoridae) of the tijuca national park, rio de janeiro, brazil. | abstractin this contribution we examine the diversity, abundance and species richness of calliphoridae in the tijuca national park, rio de janeiro, rj. specimens were collected monthly between september 2009 and august 2010, using sardines as bait, in three points: a, on the forest edge (22°56'846"s 43°17'496"w), b, 700m from it (22°57'073"s 43°17'832"w) and c, 1,200m into the forest (22°57'321"s 43°18'031"w), evaluating the indicator species at each point and the anthropogenic influences and ab ... | 2015 | 26560666 |
| effect of bait decomposition on the attractiveness to species of diptera of veterinary and forensic importance in a rainforest fragment in brazil. | insects associated with carrion can have parasitological importance as vectors of several pathogens and causal agents of myiasis to men and to domestic and wild animals. we tested the attractiveness of animal baits (chicken liver) at different stages of decomposition to necrophagous species of diptera (calliphoridae, fanniidae, muscidae, phoridae and sarcophagidae) in a rainforest fragment in brazil. five types of bait were used: fresh and decomposed at room temperature (26 °c) for 24, 48, 72 an ... | 2016 | 26547564 |
| reference gene stability of a synanthropic fly, chrysomya megacephala. | stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qpcr). a synanthropic fly, chrysomya megacephala, is a well known medical vector and forensic indicator. unfortunately, previous studies did not look at the stability of reference genes used in c. megacephala. | 2015 | 26515169 |
| unusual presence of ornidia robusta (diptera: syrphidae) causing pig myiasis in argentina. | myiasis is caused by dipterous larvae from the calliphoridae, sarcophagidae, muscidae, cuterebridae, and syrphidae families. in this work, cochliomyia hominivorax, chrysomya megacephala, and ornidia robusta were identified causing vulva, ear, and leg myiasis in pigs in tucuman province, northwestern argentina. the report of the presence of c. hominivorax and c. megacephala is very important due to their role as myiasis-causing and disease vectors. the occurrence of ornidia robusta is remarkable, ... | 2015 | 26450595 |
| body size, rather than male eye allometry, explains chrysomya megacephala (diptera: calliphoridae) activity in low light. | male chrysomya megacephala (f.) blow fly compound eyes contain an unusual area of enlarged dorsal facets believed to allow for increased light capture. this region is absent in females and has been hypothesized to aid in mate tracking in low light conditions or at greater distances. many traits used in the attraction and capture of mates are allometric, growing at different rates relative to body size. previous reports concerning c. megacephala eye properties did not include measurements of body ... | 2015 | 26411786 |
| the importance of habitat in the ecology of decomposition on rabbit carcasses in malaysia: implications in forensic entomology. | the stages of decomposition and the faunal succession on rabbit carcasses in three different habitats, namely jungle, rural, and highland areas, were studied. three new zealand white rabbit (oryctolagus cuniculus) carcasses weighing ∼2 kg were sampled daily until the decomposition process was completed. representative specimens of adult flies, larvae, pupa, and mites were collected from the carcasses and processed in the laboratory. there were differences in decomposition rate and faunal success ... | 2015 | 26336275 |
| egg developmental time and survival of chrysomya megacephala and chrysomya putoria (diptera: calliphoridae) under different temperatures. | chrysomya megacephala (f.) and chrysomya putoria (wiedemann) (diptera: calliphoridae) are considered of forensic, medical, and veterinary importance in brazil because of their necrophagous and synanthropic behaviour. the development of flies can be influenced by temperature, and species from the same genus usually have different responses to external variables. the egg development of blow fly can be a useful complementary technique to estimate the minimum postmortem interval. thus, this study ai ... | 2015 | 26335461 |
| survey of the genetic diversity of forensically important chrysomya (diptera: calliphoridae) from egypt. | minimum postmortem interval estimations of a corpse using blow fly larvae in medicolegal investigations require correct identification and the application of appropriate developmental data of the identified fly species. species identification of forensically relevant blow flies could be very difficult and time consuming when specimens are damaged or in the event of morphologically indistinguishable immature stages, which are most common at crime scenes. in response to this, an alternative, accur ... | 2015 | 26334805 |
| preliminary screening of the larvicidal effect of brevibacillus laterosporus strains against the blowfly chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae). | this study evaluated whether different strains of brevibacillus laterosporus could be used to control larvae of the blowfly chrysomya megacephala, a pest that affects both human and animal health. | 2015 | 26312933 |
| laboratory colonization of the blow flies, chrysomya megacephala (diptera: calliphoridae) and chrysomya rufifacies (diptera: calliphoridae). | chrysomya megacephala (f.) and chrysomya rufifacies (macquart) were colonized so that larval growth rates could be compared. colonies were also established to provide insight into the protein needs of adult c. rufifacies and developmental rates of the ensuing larvae. the c. megacephala and c. rufifacies laboratory colonies were reared for five and six generations, respectively, at 28°c. c. megacephala developmental mean rate from egg to adult was 20.4 ± 0.38 d. first-instar larvae emerge in 1.4 ... | 2014 | 26309267 |
| human myiasis in rural south africa is under-reported. | myiasis is the infestation of live tissue of humans and other vertebrates by larvae of flies. worldwide, myiasis of humans is seldom reported, although the trend is gradually changing in some countries. reports of human myiasis in africa are few. several cases of myiasis were recently seen at the mthatha hospital complex, mthatha, eastern cape province, south africa (sa). | 2015 | 26242532 |
| effect of juvenoids pyriproxyfen and diofenolan on embryogenesis and postembryonic development of blow fly chrysomya megacephala (diptera: calliphoridae) following egg treatment. | the blow fly chrysomya megacephala is a serious medico-veterinary pest causing myiasis in humans and animals apart from transmitting various disease-causing pathogens. being an economically important pest, it warrants successful control in the early stages without affecting the host, environment, and non-target organisms. juvenoids being safe, species and stage selective, biodegradable, and harmless to beneficial non-target organisms. treatment of freshly laid eggs of c. megacephala with juvenoi ... | 2015 | 26003811 |
| use of maggot therapy for treating a diabetic foot ulcer colonized by multidrug resistant bacteria in brazil. | this study reports the efficacy of maggot therapy in the treatment of diabetic foot ulcer infected with multidrug resistant microorganisms. a 74 year old female patient with diabetes for over 30 years, was treated with maggot therapy using larvae of chrysomya megacephala. the microbiological samples were collected to evaluate aetiology of the infection. the therapy done for 43 days resulted in a reduction of necrosis and the ulcer's retraction of 0.7 cm [2] in area. analysis of the bacteriologic ... | 2015 | 25963495 |
| reading mammal diversity from flies: the persistence period of amplifiable mammal mtdna in blowfly guts (chrysomya megacephala) and a new dna mini-barcode target. | most tropical mammal species are threatened or data-deficient. data collection is impeded by the traditional monitoring approaches which can be laborious, expensive and struggle to detect cryptic diversity. monitoring approaches using mammal dna derived from invertebrates are emerging as cost- and time-effective alternatives. as a step towards development of blowfly-derived dna as an effective method for mammal monitoring in the biodiversity hotspot of peninsular malaysia, our objectives were (i ... | 2015 | 25898278 |
| detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of chrysomya (diptera, calliphoridae) in brazil. | antibiotic-resistant bacteria in hospitals and communities increasingly threaten public health in brazil and the rest of the world. there is an urgent need for additional antimicrobial drugs. calliphorid blowfly larvae are a rich source of antimicrobial factors but the potential of neotropical species has been neglected. this preliminary study evaluates the antimicrobial activity of the native excretions/secretions of larvae of three species of brazilian calliphorids, chrysomya megacephala, chry ... | 2015 | 25817237 |
| in vitro repellent effect of tea tree (melaleuca alternifolia) and andiroba (carapa guianensis) oils on haemotobia irritans and chrysomya megacephala flies. | this study aimed to evaluate the repellent effect of tea tree (melaleuca alternifolia) and andiroba (carapa guianensis) essential oils on two species of flies (haemotobia irritans and chrysomya megacephala). for the in vitro studies, free-living adult flies were captured and reared in the laboratory. to verify the repellency effect, an apparatus was constructed where h. irritans and c. megacephala were exposed to andiroba and tea tree oils (5.0%), as well as to a known repellent (citronella, 5.0 ... | 2015 | 25801266 |
| effects of preservatives and killing methods on morphological features of a forensic fly, chrysomya megacephala (fabricius, 1794) larva. | preservation of larvae retrieved from cadavers is important in ensuring the quality and integrity of entomological specimens used for the estimation of post-mortem interval (pmi). the process of killing and preserving larvae could distort the larvae leading to inaccurate estimation of pmi. in this study, the effects of killing chrysomya megacephala larvae with hot water at different temperatures and subsequent maintenance in various preservatives were determined. larvae not killed by hot water b ... | 2014 | 25776605 |
| the developmental transcriptome of the synanthropic fly chrysomya megacephala and insights into olfactory proteins. | chrysomya megacephala (fabricius) is a prevalent and synanthropic blowfly which has two sides, for being a pathogenic vector, an efficient pollinator, a promising resource of proteins, lipids, chitosan, biofuel et al., and an important forensic indicator. moreover olfactory proteins are crucial component to function in related processes. however, the genomic platform of c. megacephala remains relatively unavailable. developmental transcriptomes of eggs, larvae from 1st instar to before pupa stag ... | 2015 | 25612629 |
| the effect of dietary fat levels on the size and development of chrysomya megacephala (diptera: calliphoridae). | variation in the type of tissue that larvae feed on can produce marked differences in developmental rate and body size, which can compromise predictions of minimum postmortem interval. a series of experiments were conducted to investigate the effect of fat content in the diet on larval growth in chrysomya megacephala (fabricius) (diptera: calliphoridae), an important forensic blowfly species in china. bionomical parameters such as body size, development time, mortality, and sex ratio were observ ... | 2014 | 25550356 |
| [morphological characteristics and gene expression of chrysomya megacephala eggs in different developmental stages]. | to research the morphological characteristics and differential gene expression of chrysomya megacephala eggs in different developmental stages. | 2014 | 25518595 |
| blow fly maggots (diptera: calliphoridae)from a human corpse in a vehicle. | correct species identification and development data of insects associated with a cadaver can help estimate the time of colonization which could be used to infer a minimal post-mortem interval (minpmi) for forensic investigations. human remains are found in a variety of locations ranging from open fields to inside automobiles. we report the investigation of blow fly larvae collected from a decomposing body located in the trunk of a car. there were two blow fly (diptera: calliphoridae) species: ac ... | 2014 | 25417501 |
| [the community succession of sarcosaphagous insects on pig carcasses in summer indoor and outdoor environment in shenzhen area]. | to explore the growing development and community succession of main sarcosaphagous insects on pig carcasses in summer indoor and outdoor environment in shenzhen area and to estimate the postmortem interval (pmi). | 2014 | 25272869 |
| the blow fly, chrysomya megacephala, and the house fly, musca domestica, as mechanical vectors of pathogenic bacteria in northeast thailand. | the oriental latrine fly, chrysomya megacephala (fabricius) (diptera: calliphoridae) and the house fly, musca domestica l., (diptera: muscidae) are synanthropic flies which are adapted to live in close association with human habitations, thereby making them likely mechanical vectors of several pathogens to humans. there were two main aims of this study. the first aim was to determine the prevalence of these two fly species from five types of human habitations including: fresh-food markets, garba ... | 2014 | 25134903 |
| an update of the blow flies (diptera: calliphoridae) of the galápagos islands, and first record of chrysomya rufifacies (macquart) from mainland ecuador. | seven species of calliphoridae are reported from the galápagos islands, ecuador: lucilia pionia (walker), l. setosa (james), l. deceptor (curran), l. eximia (wiedemann), cochliomyia macellaria (fabricius), chrysomya albiceps (wiedemann), and chrysomya megacephala (fabricius). lucilia eximia is newly recorded from the islands. lucilia sp. near pionia is recorded from the island of española. the distribution and collection records of these species are discussed and listed, and a key to their ident ... | 2013 | 25113695 |
| rates of development of immatures of three species of chrysomya (diptera: calliphoridae) reared in different types of animal tissues: implications for estimating the postmortem interval. | blowflies have major medical and sanitary importance because they can be vectors of viruses, bacteria, and helminths and are also causative agents of myiasis. also, these flies, especially those belonging to the genus chrysomya, are among the first insects to arrive at carcasses and are therefore valuable in providing data for the estimation of the minimum postmortem interval (pmimin). the pmimin can be calculated by assessing the weight, length, or development stage of blowfly larvae. lack of i ... | 2014 | 25082014 |
| population genetic structure of an invasive forensically important insect. | the forensic utility of an insect can depend in part on its population structure. although some native north american species have been characterized in this fashion, information is lacking for species that were introduced from elsewhere and that might have lower genetic diversity and less geographic differentiation. we surveyed chrysomya megacephala, an asian fly present in the continental usa since the 1980s. amplified fragment length polymorphism profiles were generated from adult insects col ... | 2014 | 24934687 |
| an evaluation of sampling methods used to produce insect growth models for postmortem interval estimation. | many authors produced carrion insect development data for predicting the age of an insect from a corpse. under some circumstances, this age value is a minimum postmortem interval. there are no standard protocols for such experiments, and the literature includes a variety of sampling methods. to our knowledge, there has been no investigation of how the choice of sampling method can be expected to influence the performance of the resulting predictive model. we calculated 95 % inverse prediction co ... | 2015 | 24929639 |
| a fresh liver agar substrate for rearing small numbers of forensically important blow flies (diptera: calliphoridae). | forensically important calliphorids can be reared on a mixture of beef liver and agar. small pieces of meat, especially fresh or frozen beef liver, will desiccate in 2-6 h, but this simple-to-make feeding substrate remains moist for at least 12 h at 25 and 30 degrees c without desiccation, even in small (5 g) amounts. we determined the survivorship of small numbers of chrysomya megacephala (f.) (first-instar larvae to adult eclosion) raised on 5 g of liver agar and fresh beef liver. we found tha ... | 2014 | 24897867 |
| isolation of toxocara eggs from flies in northeast thailand. | flies are natural carriers of pathogens and play a considerable role in the mechanical transmission of many pathogens, such as viruses, fungi, bacteria, and parasites, in various regions of the world. | 2014 | 24851561 |
| antibiotic resistance patterns of enterococcus spp. isolated from musca domestica and chrysomya megacephala in ubon ratchathani. | the housefly musca domestica and the blowfly chrysomya megacephala are found worldwide and are medically significant as mechanical vectors of various pathogens from unsanitary locations to food, resulting in diseases in humans. | 2014 | 24851557 |
| first record of the blow fly chrysomya megacephala (diptera: calliphoridae) on a southern atlantic island: implications for disease transmission in a protected environment. | 2014 | 24820579 | |
| influence of paraquat on chrysomya megacephala (fabricius) (diptera: calliphoridae) infesting minced-beef substrates in kelantan, malaysia. | this study investigated the influence of paraquat, a prevalent poison used by suicides, on initial oviposition and development of chrysomya megacephala (fabricius) using minced-beef substrates. paraquat in lethal dose for human (40 mg/kg), two times the lethal dose (80 mg/kg) and five times the lethal dose (200 mg/kg) were mixed thoroughly with respective minced-beef substrates (1 kg each) that were decomposed in a shaded habitat fully protected from rain. results of four replications of the abo ... | 2014 | 24745083 |
| effects of ketamine on the development of forensically important blowfly chrysomya megacephala (f.) (diptera: calliphoridae) and its forensic relevance. | this study investigated effects of ketamine on the development of chrysomya megacephala (diptera: calliphoridae) at three different temperatures. larvae of the c. megacephala were exposed to different concentrations of drugs and temperatures. the larval lengths, weights, and developmental durations of each stage were observed. this study demonstrated that ketamine, low temperature, and their synergistic action significantly suppressed the development of c. megacephala (p < 0.001). the time that ... | 2014 | 24673587 |
| developmental rates of immatures of three chrysomya species (diptera: calliphoridae) under the effect of methylphenidate hydrochloride, phenobarbital, and methylphenidate hydrochloride associated with phenobarbital. | entomotoxicology is focused on obtaining data on necrophagous entomofauna, for criminal investigations purposes. this study aimed to evaluate the effect of different concentrations of methylphenidate hydrochloride, phenobarbital, and their association on the developmental rate, larval and pupal survivorship, and the interval of emergence of adults of chrysomya albiceps (wiedemann), chrysomya megacephala (fabricius), and chrysomya putoria (wiedemann) (diptera: calliphoridae). considering the ther ... | 2014 | 24633905 |
| species composition of carrion blow flies in northern thailand: altitude appraisal. | distribution and occurrence of blow flies of forensic importance was performed during 2007 and 2008 in chiang mai and lampang provinces, northern thailand. surveys were conducted in forested areas for 30 minutes using a sweep net to collected flies attracted to a bait. a total of 2,115 blow flies belonging to six genera and 14 species were collected; chrysomya megacephala (fabricius) (44.7%), c. pinguis (walker) (15.1%), c. chani kurahashi (9.3%), c. thanomthini kurahashi & tumrasvin (0.3%); ach ... | 2017 | 24626423 |
| an atpase inhibitory peptide with antibacterial and ion current effects. | an 84-residue bactericidal peptide, psk, was purified from a chrysomya megacephala fly larvae preparation. its amino acid sequence is similar to that of a previously reported larval peptide of the drosophila genus (sk84) noticed for its anticancer and antimicrobial properties. the psk sequence is also homologous to mitochondrial atpase inhibitors from insects to humans (35-65% sequence identity), indicating an intracellular protein target and possible mechanism for psk. it contains a cluster of ... | 2014 | 24613831 |
| multi-drug resistant gram-negative enteric bacteria isolated from flies at chengdu airport, china. | we collected flies from chengdu shuangliu international airport to examine for the presence of bacteria and to determine the sensitivity patterns of those bacteria. a total of 1,228 flies were collected from 6 sites around chengdu shuangliu international airport from april to september 2011. the predominant species was chrysomya megacephala (n=276, 22.5%). antimicrobial-resistant gram-negative enteric bacteria (n=48) were isolated from flies using macconkey agar supplemented with cephalothin (20 ... | 2013 | 24450236 |
| multiple colonization of a cadaver by insects in an indoor environment: first record of fannia trimaculata (diptera: fanniidae) and peckia (peckia) chrysostoma (sarcophagidae) as colonizers of a human corpse. | we describe here a case of multiple colonization of a male cadaver found indoors in the municipality of jaboatao dos guararapes, brazil. the body was colonized by six species of diptera: chrysomya albiceps, chrysomya megacephala, chrysomya putoria (calliphoridae), megaselia scalaris (phoridae), fannia trimaculata (fanniidae), and peckia (peckia) chrysostoma (sarcophagidae). the most abundant species were c. albiceps (65.0 % of all emerged adults) and c. megacephala (18.6 %). the case illustrates ... | 2014 | 24218014 |
| adult carrion arthropod community in a tropical rainforest of malaysia: analysis on three common forensic entomology animal models. | decomposing carrion provides a temporary microhabitat and food source for a distinct community of organisms. arthropods constitute a major part of this community and can be utilized to estimate the postmortem interval (pmi) of cadavers during criminal investigations. however, in malaysia, knowledge of carrion arthropod assemblages and their succession is superficial. therefore, a study on three types of forensic entomology animal model was conducted from 27 september 2010 to 28 october 2010 in a ... | 2013 | 24189678 |
| studies on the potential and public health importance of non-biting synanthropic flies in the mechanical transmission of human enterohelminths. | this study was aimed at examining the potential of non-biting synanthropic filth flies to acquire naturally eggs of human intestinal helminths from unsanitary sites, and its attendant public health importance. | 2013 | 24128876 |
| life history data on the fly parasitoids aleochara nigra kraatz and a. asiatica kraatz (coleoptera: staphylinidae), and their potential application in forensic entomology. | knowledge of the developmental time of the immature stages of necrophagous flies has been the main tool for estimating minimum post-mortem intervals (min pmis) in forensic entomology. many parasitic insects can alter the development of immature stages of flies and thus affect min pmi estimates. the larvae of most species of aleochara rove beetles are ectoparasitoids of the pupae of cyclorrhapha flies. among them, some species that parasitise necrophagous flies may have forensic importance. two t ... | 2013 | 24053864 |
| time of death revealed by hydrocarbons of empty puparia of chrysomya megacephala (fabricius) (diptera: calliphoridae): a field experiment. | determination of the postmortem interval (pmi) is crucial for investigating homicide. however, there are currently only limited methods available. especially, once the pmi exceeds the duration of pre-adult development of the flies with the adult emergence, its determination is very approximate. herein, we report the regular changes in hydrocarbon composition during the weathering process of the puparia in the field in chrysomya megacephala (fabricius) (diptera: calliphoridae), one of the common ... | 2013 | 24039855 |
| umbilical myiasis associated with staphylococcus aureus sepsis in a neonate. | myiasis is an infection of live mammalian tissue by the larvae of dipteran flies and commonly found in the tropics and subtropics. it usually infects domestic and wild animals, but sometimes also affects human. umbilical cord myiasis in the neonatal period associated with sepsis is a rare occurrence with few reported cases in the literature. in this article, authors report a 7-day-old neonate from a rural area presented with neonatal sepsis due to umbilical myiasis caused by fly larval form of b ... | 2012 | 24027685 |
| evaluation of an i-box wind tunnel model for assessment of behavioral responses of blow flies. | the behavioral response of flies to olfactory cues remains the focus of many investigations, and wind tunnels have sometimes been employed for assessment of this variable in the laboratory. in this study, our aim was to design, construct, and operate a new model of i-box wind tunnel with improved efficacy, highlighting the use of a new wind tunnel model to investigate the behavioral response of the medically important blow fly, chrysomya megacephala (fabricius). the i-box dual-choice wind tunnel ... | 2013 | 23979494 |
| morphology and identification of fly eggs: application in forensic entomology. | fly eggs found in corpses can be used as entomological evidence in forensic investigation. this study aims to investigate the morphology of forensically important fly eggs. eggs of chrysomya rufifacies, chrysomya megacephala, chrysomya pinguis, chrysomya nigripes, hypopygiopsis tumrasvini, lucilia cuprina, lucilia porphyrina and musca domestica were examined using 1% potassium permanganate solution for 1 min. morphometric analysis revealed that the mean length of hy. tumrasvini (1.63 mm) and c. ... | 2013 | 23959498 |
| molecular identification of malaysian chrysomya megacephala (fabricius) and chrysomya rufifacies (macquart) using life stage specific mitochondrial dna. | dna identification of blow fly species can be a very useful tool in forensic entomology. one of the potential benefits that mitochondrial dna (mtdna) has offered in the field of forensic entomology is species determination. conventional identification methods have limitations for sibling and closely related species of blow fly and stage and quality of the specimen used. this could be overcome by dna-based identification methods using mitochondrial dna which does not demand intact or undamaged sp ... | 2013 | 23959486 |
| diversity and synanthropy of calliphoridae (diptera) in the region of rio claro, sp, brazil. | dipteran blowflies (calliphoridae) are of great medical and hygienic importance as vectors of pathogens and as parasites of living and dead tissue, and their association with carrion allows their use in forensic entomology. the objective of this study was to determine the synanthropic index of adult calliphoridae (diptera) collected in rio claro, são paulo. sampling occurred between september 2009 and august 2010. traps baited with sardines, beef liver, and minced meat were assessed for five con ... | 2012 | 23950050 |
| physicochemical properties and antioxidant activity of chitosan from the blowfly chrysomya megacephala larvae. | in the present study, we extracted the chitosan from the larvae of blowfly chrysomya megacephala, a new source of insect chitosan, using chemical methods. we evaluated the physical properties of the blowfly chitosan using a variety of approaches, including preliminary color-change identification, molecular weight determination, elemental analysis (ea), fourier transform infrared spectroscopy (ftir), solid-state (13)c cross-polarization and magic-angle-spinning nuclear magnetic resonance spectros ... | 2013 | 23792633 |
| review of forensically important entomological specimens collected from human cadavers in malaysia (2005-2010). | forensic entomological specimens collected from human decedents during crime scene investigations in malaysia in the past 6 years (2005-2010) are reviewed. a total of 80 cases were recorded and 93 specimens were collected. from these specimens, 10 species of cyclorrphagic flies were identified, consisting of chrysomya rufifacies (macquart) -38 specimens (40.86%), chrysomya megacephala (fabricius) -36 specimens (38.70%), chrysomya villeneuvi (patton) -2 specimens (2.15%), chrysomya nigripes (aube ... | 2013 | 23756518 |
| analysis of the transcriptome of blowfly chrysomya megacephala (fabricius) larvae in responses to different edible oils. | chrysomya megacephala (fabricius), a prevalent necrophagous blowfly that is easily mass reared, is noted for being a mechanical vector of pathogenic microorganisms, a pollinator of numerous crops, and a resource insect in forensic investigation in the postmortem interval. in the present study, in order to comprehensively understand the physiological and biochemical functions of c. megacephala, we performed rna-sequencing and digital gene expression (dge) profiling using solexa/illumina sequencin ... | 2013 | 23690992 |
| dna typing of calliphorids collected from human corpses in malaysia. | estimation of post-mortem interval (pmi) is crucial for time of death determination. the advent of dna-based identification techniques forensic entomology saw the beginning of a proliferation of molecular studies into forensically important calliphoridae (diptera). the use of dna to characterise morphologically indistinguishable immature calliphorids was recognised as a valuable molecular tool with enormous practical utility. the local entomofauna in most cases is important for the examination o ... | 2013 | 23665717 |
| applicability of partial characterization of cytochrome oxidase i in identification of forensically important flies (diptera) from china and egypt. | precise species identification of every insect sample collected from criminal scenes play an essential role in the accurate estimation of postmortem interval. the morphological similarity poses a great challenge for forensic entomologists. dna-based method can be used as a supplemental means of morphological method. in the present study, we demonstrate the applicability of the 304-bp cytochrome oxidase i gene fragment in molecular identification of forensically important diptera. we analyzed 75 ... | 2013 | 23661266 |
| molecular characterization of the carbon dioxide receptor in the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae). | the blowfly chrysomya megacephala (fabricius) (diptera: calliphoridae) cannot only act as a mechanical vector of various pathogens, but also infest man and animals causing human health problems and economic losses in the livestock and fish industries. as in other insects, olfaction of this species plays an important role in host location and is presumably mediated via transmembrane receptor signaling pathways. here, we isolate and characterize cmeggr1 and cmeggr2, two new members of the chemosen ... | 2013 | 23604565 |
| using the developmental gene bicoid to identify species of forensically important blowflies (diptera: calliphoridae). | identifying species of insects used to estimate postmortem interval (pmi) is a major subject in forensic entomology. because forensic insect specimens are morphologically uniform and are obtained at various developmental stages, dna markers are greatly needed. to develop new autosomal dna markers to identify species, partial genomic sequences of the bicoid (bcd) genes, containing the homeobox and its flanking sequences, from 12 blowfly species (aldrichina grahami, calliphora vicina, calliphora l ... | 2013 | 23586044 |