Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| dsrna targeting calmodulin reveal a potential target for pest management of nilaparvata lugens. | calmodulin (cam) is an essential protein in cellular activity and play important roles in many processes in insect development. rnai have been hypothesized to be promising methods for pest control. however, the sequence and function of cam in nilaparvata lugens is unknown. futhermore, the dsrna target to gene with higher similarity sequence in pest control is still unavailable. | 2018 | 29381254 |
| ribosomal protein l18 is an essential factor that promote rice stripe virus accumulation in small brown planthopper. | rice stripe virus (rsv) transmitted by the vector, small brown planthopper (sbph), can cause a severe rice disease. the nucleocapsid (n) protein is the major component of rsv ribonucleoprotein particles (rnps), and it plays important roles in viral persistent-propagative transmission by sbph. to gain further insights into the vector components enabling rsv transmission, a gal4-based yeast two-hybrid system was utilized to find unknown vector factors that interact with the n protein. thirteen dif ... | 2018 | 29374519 |
| corrigendum: map-based cloning and characterization of bph29, a b3 domain-containing recessive gene conferring brown planthopper resistance in rice. | 2018 | 29373714 | |
| characterization of nlhox3, an essential gene for embryonic development in nilaparvata lugens. | hox genes encode transcriptional regulatory proteins that control axial patterning in all bilaterians. the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae), is a destructive insect pest of rice plants in asian countries. during analysis of the n. lugens transcriptome, we identified a hox3-like gene (nlhox3) that was highly and specifically expressed in the embryonic stage. we performed functional analysis on the gene to identify its roles in embryonic development and its pote ... | 2018 | 29369417 |
| effects of seed mixture sowing with transgenic bt rice and its parental line on the population dynamics of target stemborers and leafrollers, and non-target planthoppers. | the widespread planting of insect-resistant crops has caused a dramatic shift in agricultural landscapes, thus raising concerns about the potential impacts on both target and non-target pests. in this study, we examined the potential effects of intra-specific seed mixture sowing with transgenic bt rice (bt) and its parental non-transgenic line (nt) [100% bt rice (bt100 ), 5% nt+95% bt (nt05 bt95 ), 10% nt+90% bt (nt10 bt90 ), 20% nt+80% bt (nt20 bt80 ), 40% nt+60% bt (nt40 bt60 ), and 100% nt ri ... | 2018 | 29368405 |
| bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice. | the brown planthopper (bph) and white-backed planthopper (wbph) are the most destructive insect pests of rice, and they pose serious threats to rice production throughout asia. thus, there are urgent needs to identify resistance-conferring genes and to breed planthopper-resistant rice varieties. here we report the map-based cloning and functional analysis of bph6, a gene that confers resistance to planthoppers in rice. bph6 encodes a previously uncharacterized protein that localizes to exocysts ... | 2018 | 29358653 |
| a neutral ceramidase, nlncdase, is involved in the stress responses of brown planthopper, nilaparvata lugens (stål). | ceramidases (cdases) are vital enzymes involved in the biosynthesis of sphingolipids, which are essential components of eukaryotic membranes. the function of these enzymes in insects, however, is poorly understood. we identified a neutral ceramidase (nlncdase) from the brown planthopper, nilaparvata lugens, one of the most destructive hemipteran pests of rice. the c12-ceramide was the most preferred substrate for the nlncdase enzyme. the activity of the nlncdase enzyme was highest in the neutral ... | 2018 | 29348442 |
| neofunctionalization of duplicated p450 genes drives the evolution of insecticide resistance in the brown planthopper. | gene duplication is a major source of genetic variation that has been shown to underpin the evolution of a wide range of adaptive traits [1, 2]. for example, duplication or amplification of genes encoding detoxification enzymes has been shown to play an important role in the evolution of insecticide resistance [3-5]. in this context, gene duplication performs an adaptive function as a result of its effects on gene dosage and not as a source of functional novelty [3, 6-8]. here, we show that dupl ... | 2018 | 29337073 |
| effects of seed mixture sowing with resistant and susceptible rice on population dynamics of target planthoppers and non-target stemborers and leaffolders. | the widespread planting of insect-resistant crops has caused a dramatic shift in agricultural landscapes, thus raising concerns about the potential impact on both target and non-target pests worldwide. in this study, we examined the potential effects of six seed-mixture ratios of insect-resistant dominance (100%-r100, 95%-s05r95, 90%-s10r90, 80%-s20r80, 60%-s40r60, and 0%-s100) on target and non-target pests in a 2-year field trial in southern china. | 2018 | 29330915 |
| silicon amendment to rice plants contributes to reduced feeding in a phloem-sucking insect through modulation of callose deposition. | silicon (si) uptake by poaceae plants has beneficial effects on herbivore defense. increased plant physical barrier and altered herbivorous feeding behaviors are documented to reduce herbivorous arthropod feeding and contribute to enhanced plant defense. here, we show that si amendment to rice (oryza sativa) plants contributes to reduced feeding in a phloem feeder, the brown planthopper (nilaparvata lugens, bph), through modulation of callose deposition. we associated the temporal dynamics of bp ... | 2018 | 29321899 |
| marker assisted pyramiding of bph6 and bph9 into elite restorer line 93-11 and development of functional marker for bph9. | the brown planthopper (bph) has become the most destructive and a serious threat to the rice production in asia. breeding the resistant varieties with improved host resistance is the most effective and ecosystem-friendly strategy of bph biological management. as host resistance was always broken down by the presence of the upgrading bph biotype, the more resistant varieties with novel resistance genes or pyramiding known identified bph resistance genes would be needed urgently for higher resista ... | 2017 | 29282566 |
| transferrin family genes in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae) in response to three insecticides. | transferrins are involved in iron metabolism, immunity, xenobiotics tolerance, and development in eukaryotic organisms including insects. however, little is known about the relationship between transferrins and insecticide toxicology and resistance. three transferrin family genes, nltsf1, nltsf2, and nltsf3, of the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae)a major insect pest of rice field in asia, had been identified and characterized in this study. quantitative polym ... | 2017 | 29272461 |
| role of broad-complex (br) and krüppel homolog 1 (kr-h1) in the ovary development of nilaparvata lugens. | ovarian development plays an important role in the life history of insects and is crucial for control of the insect population. the metamorphosis of an insect is precisely regulated by the interaction of the juvenile hormone and ecdysone. to understand the role of nlbr and nlkr-h1 in ovary development, we used rna interference (rnai) to down-regulate the expression of broad-complex (br) and krüppel homolog 1 (kr-h1), two important down-stream transcription factors of juvenile hormone and ecdyson ... | 2017 | 29270133 |
| functional study of cytochrome p450 enzymes from the brown planthopper (nilaparvata lugens stål) to analyze its adaptation to bph-resistant rice. | plant-insect interactions constitute a complex of system, whereby plants synthesize toxic compounds as the main defense strategy to combat herbivore assault, and insects deploy detoxification systems to cope with toxic plant compounds. cytochrom p450s are among the main detoxification enzymes employed by insects to combat the chemical defenses of host plants. in this study, we used nilaparvata lugens (bph) to constitute an ideal system for studying plant-insect interactions. by feeding bphs with ... | 2017 | 29249980 |
| crispr/cas9-mediated knockout of two eye pigmentation genes in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | the brown planthopper nilaparvata lugens is one of the most destructive insect pests in asia, demonstrating high fertility and causing huge crop losses by sucking sap of rice as well as transmitting viruses. however, functional genomic studies on n. lugens are seriously constrained by lack of genetic tools. here, we employed two eye pigmentation genes to generate germ-line mutations in n. lugens using the crispr/cas9 (clustered regularly interspaced palindromic repeats/crispr-associated) system. ... | 2017 | 29241845 |
| an adenylyl cyclase like-9 gene (nlac9) influences growth and fecundity in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae). | the camp/pka intracellular signaling pathway is launched by adenylyl cyclase (ac) conversion of adenosine triphosphate (atp) to 3', 5'-cyclic amp (camp) and camp-dependent activation of pka. although this pathway is very well known in insect physiology, there is little to no information on it in some very small pest insects, such as the brown planthopper (bph), nilaparvata lugens stål. bph is a destructive pest responsible for tremendous crop losses in rice cropping systems. we are investigating ... | 2017 | 29236776 |
| characterization of a nilaparvata lugens (stål) brummer gene and analysis of its role in lipid metabolism. | the brummer (bmm) genes encode the lipid storage droplet-associated triacylglycerols (tag) lipases, which belong to the brummer/nutrin subfamily. these enzymes hydrolyze the ester bonds in tag in lipid metabolism and act in insect energy homeostasis. exposure to some agricultural chemicals leads to increased fecundity, which necessarily involves lipid metabolism, in some planthopper species. however, the biological roles of bmm in planthopper lipid storage and mobilization have not been investig ... | 2017 | 29230863 |
| identification of virus-derived sirnas and their targets in rbsdv-infected rice by deep sequencing. | rna interference (rnai) is a conserved mechanism against viruses in plants and animals. it is thought to inactivate the viral genome by producing virus-derived small interfering rnas (vsirnas). rice black-streaked dwarf virus (rbsdv) is transmitted to plants by the small brown planthopper (laodelphax striatellus), and seriously threatens production of rice in east asia, particularly oryza sativa japonica subspecies. through deep sequencing, genome-wide comparisons of rbsdv-derived vsirnas were m ... | 2017 | 29215744 |
| design, synthesis and bioactivities of novel isoxazole-containing pyrazole oxime derivatives. | in this study, in order to find novel biologically active pyrazole oxime derivatives, twenty-eight new pyrazole oxime compounds containing a substituted isoxazole ring were synthesized and evaluated for their acaricidaland insecticidal activities. bioassays exhibited that some target compounds indicated good acaricidal and insecticidal activities against tetranychus cinnabarinus, aphis medicaginis, mythimna separata, and nilaparvata lugens. especially, compounds 9c, 9h, 9u, and 9v showed 100.00% ... | 2017 | 29186906 |
| identification of a sugar gustatory receptor and its effect on fecundity of the brown planthopper nilaparvata lugens. | in insects, the gustatory system plays a crucial role in multiple physiological behaviors, including feeding, toxin avoidance, courtship, mating and oviposition. gustatory stimuli from the environment are recognized by gustatory receptors. to date, little is known about the function of gustatory receptors in agricultural pest insects. in this study, we cloned a sugar gustatory receptor gene, nlgr11, from the brown planthopper (bph), nilaparvata lugens (stål), a serious pest of rice in asia; we t ... | 2017 | 29178612 |
| cry64ba and cry64ca, two etx/mtx2-type bacillus thuringiensis insecticidal proteins active against hemipteran pests. | genetically modified crops that express insecticidal bacillus thuringiensis (bt) proteins have become a primary approach for control of lepidopteran (moth) and coleopteran (beetle) pests that feed by chewing the plants. however, the sap-sucking insects (hemiptera) are not particularly susceptible to bt toxins. in this study, we describe two cry toxins (cry64ba and cry64ca) from bt strain 1012 that showed toxicity against two important hemipteran rice pests, laodelphax striatellus and sogatella f ... | 2018 | 29150505 |
| investigation of alimentary canal ultrastructure following knockdown of the dicer-2 gene in planthoppers reveals the potential pathogenicity of southern rice black streaked dwarf virus to its insect vector. | an increasing number of studies are suggesting that plant viruses, including southern rice black-streaked dwarf virus (srbsdv), can adversely affect biological characteristics of insect vectors by unknown mechanisms. to study the adverse effect of srbsdv at cellular level on the insect vector, we promoted viral infection by the disruption of the small interfering rna (sirna) pathway. the transmission electron microscopy was utilized to describe the ultrastructural changes that occurred in insect ... | 2017 | 29141205 |
| genome sequence of the small brown planthopper, laodelphax striatellus. | laodelphax striatellus fallén (hemiptera: delphacidae) is one of the most destructive rice pests. l. striatellus is different from 2 other rice planthoppers with a released genome sequence, sogatella furcifera and nilaparvata lugens, in many biological characteristics, such as host range, dispersal capacity, and vectoring plant viruses. deciphering the genome of l. striatellus will further the understanding of the genetic basis of the biological differences among the 3 rice planthoppers. | 2017 | 29136191 |
| a mucin-like protein of planthopper is required for feeding and induces immunity response in plants. | the brown planthopper, nilaparvata lugens, is a pest that threatens rice (oryza sativa) production worldwide. while feeding on rice plants, planthoppers secrete saliva, which plays crucial roles in nutrient ingestion and modulating plant defense responses, although the specific functions of salivary proteins remain largely unknown. we identified an n. lugens-secreted mucin-like protein (nlmlp) by transcriptome and proteome analyses and characterized its function, both in brown planthopper and in ... | 2018 | 29133370 |
| resistance monitoring and cross-resistance role of cyp6cw1 between buprofezin and pymetrozine in field populations of laodelphax striatellus (fallén). | monitoring resistance and investigating insecticide resistance mechanisms are necessary for controlling the small brown planthopper, laodelphax striatellus. the susceptibility to four common insecticides of l. striatellus collected from jiangsu, anhui, zhejiang and jilin provinces of china in 2015 was monitored. the results showed that all field populations remained susceptible to chlorpyrifos and thiamethoxam with resistance ratios (rrs) of 2.3- to 9.5 and 1.6- to 3.3, respectively, while the i ... | 2017 | 29116195 |
| silencing pyruvate kinase (nlpyk) leads to reduced fecundity in brown planthoppers, nilaparvata lugens (stål) (hemiptera: delphacidae). | pyruvate kinase (pyk) operates in the glycolytic pathway, responsible for regulating the balance between glycolysis and gluconeogenesis. the previous work indicates pyk acts in development of drosophila embryos and in embryonic muscle growth, from which it may be inferred that pyk acts in insect fecundity. more to the point, as a central enzyme in the glycolytic pathway, pyk acts in many energy-spending functions in most organisms. on the background findings that triazophos (tzp) stimulates fecu ... | 2017 | 29114912 |
| overexpression of osrrk1 changes leaf morphology and defense to insect in rice. | it has been reported that the receptor-like cytoplasmic kinases (rlcks) regulate many biological processes in plants, but only a few members have been functionally characterized. here, we isolated a rice gene encoding atrrk1 homology protein kinase, osrrk1, which belongs to the rlck vi subfamily. osrrk1 transcript accumulated in many tissues at low to moderate levels and at high levels in leaves. overexpression of osrrk1 (oe-osrrk1) caused adaxial rolling and erect morphology of rice leaves. in ... | 2017 | 29114253 |
| combined transcriptomic/proteomic analysis of salivary gland and secreted saliva in three planthopper species. | the planthoppers are piercing-sucking pests that continuously inject saliva into host plants using specialized stylets. however, knowledge on the constituent and function of planthopper saliva proteins was still limited. in this study, the transcriptomic and proteomic approach were adopted to characterize the composition of salivary glands and their secreted saliva in three planthoppers, respectively. gene repertoires of salivary glands in brown planthopper (nilaparvata lugens, bph), white-backe ... | 2018 | 29109049 |
| characterization of heat shock protein 70 transcript from nilaparvata lugens (stål): its response to temperature and insecticide stresses. | the brown planthopper, nilaparvata lugens, possesses a strong adaptability to extreme temperature and insecticide stresses. heat shock proteins (hsps) are highly conserved molecular chaperones and play a pivotal role in response to various environmental stresses in insects. however, little is known about the response of hsps to stresses in n. lugens. in the present study, an inducible hsp70 (nlhsp70) was isolated from this insect and transcriptional expression patterns of nlhsp70 under temperatu ... | 2017 | 29107232 |
| influence of the rdl a301s mutation in the brown planthopper nilaparvata lugens on the activity of phenylpyrazole insecticides. | we discovered the a301s mutation in the rdl gaba-gated chloride channel of fiprole resistant rice brown planthopper, nilaparvata lugens populations by dna sequencing and snp calling via rnaseq. ethiprole selection of two field n. lugens populations resulted in strong resistance to both ethiprole and fipronil and resulted in fixation of the a301s mutation, as well as the emergence of another mutation, q359e in one of the selected strains. to analyse the roles of these mutations in resistance to p ... | 2017 | 29107231 |
| the coiled-coil and nucleotide binding domains of brown planthopper resistance14 function in signaling and resistance against planthopper in rice. | brown planthopper resistance14 (bph14), the first planthopper resistance gene isolated via map-based cloning in rice (oryza sativa), encodes a coiled-coil, nucleotide binding site, leucine-rich repeat (cc-nb-lrr) protein. several planthopper and aphid resistance genes encoding proteins with similar structures have recently been identified. here, we analyzed the functions of the domains of bph14 to identify molecular mechanisms underpinning bph14-mediated planthopper resistance. the cc or nb doma ... | 2017 | 29093216 |
| comparative proteomic analysis of susceptible and resistant rice plants during early infestation by small brown planthopper. | the small brown planthopper (laodelphax striatellus fallén, homoptera, delphacidae-sbph) is one of the major destructive pests of rice (oryza sativa l.). understanding on how rice responds to sbph infestation will contribute to developing strategies for sbph control. however, the response of rice plant to sbph is poorly understood. in this study, two contrasting rice genotypes, pf9279-4 (sbph-resistant) and 02428 (sbph-susceptible), were used for comparative analysis of protein profiles in the l ... | 2017 | 29089949 |
| molecular characterization, spatial-temporal expression and magnetic response patterns of iron-sulfur cluster assembly1 (isca1) in the rice planthopper, nilaparvata lugens. | the mechanisms of magnetoreception have been proposed as the magnetite-based, the chemical radical-pair and biocompass model, in which magnetite particles, the cryptochrome (cry) or iron-sulfur cluster assembly 1 (isca1) may be involved. however, little is known about the association among the molecules. here we investigated the molecular characterization and the mrna expression of isca1 in different developmental stages, tissues and magnetic fields in the migratory brown planthopper (bph), nila ... | 2017 | 29063672 |
| regional population differences of the brown planthopper (nilaparvata lugens stål) in cambodia using genotyping-by-sequencing. | the brown planthopper nilaparvata lugens stål (bph) can be found year-round in tropical region and causes severe damage to rice. although there has been documented bph damage to rice crops in the past decade in cambodia, the extent of this epidemic is poorly understood. here, we examined the time variation of bph population in the abundance of morphotypes in 13 main rice-producing provinces (86 sites) by aspirator method and in the takeo province (five sites) by yellow sticky trap method. at lea ... | 2017 | 29061206 |
| characterization and functional analysis of a carboxylesterase gene associated with chlorpyrifos resistance in nilaparvata lugens (stål). | the widespread and extensive application of insecticides have promoted the development of resistance in the brown planthopper nilaparvata lugens (stål), one of the most important rice pests in asia. to better understand the underlying molecular mechanisms of metabolic resistance to insecticides, a chlorpyrifos-resistant (cr) strain of n. lugens was selected and its possible resistance mechanism was investigated. synergistic tests using carboxylesterases (cares) inhibitor triphenyl phosphate (tpp ... | 2017 | 29054582 |
| a simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs. | rice black-streaked dwarf virus (rbsdv), an important rice virus, is transmitted by vector small brown planthopper (sbph) in a persistent manner, but not transovarial transmission. in order to obtain viruliferous sbph nymphs for relevant research, a simple and reliable method was developed, through allowing sbph adults laying eggs on rbsdv-infected rice plants. the results showed the hatching nymphs on diseased plants could early acquire virus, and the virus was detected in 2nd-instar nymphs fro ... | 2018 | 29050971 |
| study on the effect of wing bud chitin metabolism and its developmental network genes in the brown planthopper, nilaparvata lugens, by knockdown of tre gene. | the brown planthopper, nilaparvata lugens is one of the most serious pests of rice, and there is so far no effective way to manage this pest. however, rna interference not only can be used to study gene function, but also provide potential opportunities for novel pest management. the development of wing plays a key role in insect physiological activities and mainly involves chitin. hence, the regulating role of trehalase (tre) genes on wing bud formation has been studied by rnai. in this paper, ... | 2017 | 29033849 |
| insulin-like peptides and dna/trna methyltransferases are involved in the nutritional regulation of female reproduction in nilaparvata lugens (stål). | insulin-like peptides (ilps) sense and transduce nutritional information and are linked to female reproduction in many insect species. our previous studies have shown that "target of rapamycin" (tor) pathway functions through juvenile hormone (jh) to regulate amino acids-mediated vitellogenesis in the brown planthopper, nilaparvata lugens, one of the most destructive rice pests in asia. recent reports have demonstrated that dna methyltransferases (dnmts) are also involved in female reproduction ... | 2018 | 28993285 |
| resistance to cycloxaprid in laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits. | cycloxaprid is a new oxabridged cis-configuration neonicotinoid insecticide, the resistance development potential and underlying resistance mechanism of which were investigated in the small brown planthopper, laodelphax striatellus (fallén), an important agricultural pest of rice. | 2017 | 28991400 |
| expression and rna interference of ribosomal protein l5 gene in nilaparvata lugens (hemiptera: delphacidae). | the ribosomal proteins play important roles in the growth and development of organisms. this study aimed to explore the function of nlrpl5 (genbank kx379234), a ribosomal protein l5 gene, in the brown planthopper nilaparvata lugens. the open reading frame of nlrpl5 was cloned from n. lugens based on a previous transcriptome analysis. the results revealed that the open reading frame of nlrpl5 is of 900 bp, encoding 299 amino acid residues. the reverse transcription quantitative pcr results sugges ... | 2017 | 28973571 |
| tra-2 mediates cross-talk between sex determination and wing polyphenism in female nilaparvata lugens. | sexual dimorphism and wing polyphenism are important and evolutionarily conserved features of many insect species. in this article, we found a cross-talk linking sexual differentiation with wing polyphenism in the brown planthopper (bph) nilaparvata lugens (order: hemiptera). knockdown of the sex determination gene transformer-2 in n. lugens (nltra-2) in nymph caused females to develop into infertile pseudomales containing undeveloped ovaries. whereas males treated with dsnltra-2 exhibited norma ... | 2017 | 28951528 |
| phf7, a novel male gene influences female fecundity and population growth in nilaparvata lugens stål (hemiptera: delphacidae). | phf7 exhibits male-specific expression in early germ cells, germline stem cells and spermatogonia in insects, and its expression promotes spermatogenesis in germ cells when they are present in a male soma. however, the influence of male-specific phf7 on female reproductive biology via mating remains unclear. thus, we investigated the potential impacts of male phf7, existed in seminal fluid of nilaparvata lugens (nlphf7), on fecundity and population growth via mating. our results revealed that su ... | 2017 | 28912601 |
| insecticide susceptibilities of the two rice planthoppers nilaparvata lugens and sogatella furcifera in east asia, the red river delta, and the mekong delta. | the two rice planthoppers, nilaparvata lugens and sogatella furcifera, have different life cycles in the regions of east asia, the red river delta, and the mekong delta. the susceptibilities of these species to a range of insecticides have not previously been compared among the three regions over multiple years. here, we describe the differences and similarities in insecticide susceptibilities of the two species among the three regions in 2006-2011. | 2018 | 28898522 |
| silencing of juvenile hormone epoxide hydrolase gene (nljheh) enhances short wing formation in a macropterous strain of the brown planthopper, nilaparvata lugens. | the rice brown planthopper, nilaparvata lugens, is an important migratory pest in many rice planting areas of asia. the typical wing dimorphism of n. lugens gives them flexibility to adapt to different environmental cues. as an important hormone in the insect's endocrine regulation, juvenile hormone (jh) has previously been shown to participate in the wing morph determination of n. lugens. in this paper, we investigated the possible wing morph determination roles of two jh metabolic enzymes, jh ... | 2017 | 28867330 |
| identification and fine mapping of a new gene, bph31 conferring resistance to brown planthopper biotype 4 of india to improve rice, oryza sativa l. | rice (oryza sativa l.) is the staple food for more than 3.5 billion people, mainly in asia. brown planthopper (bph) is one of the most destructive insect pests of rice that limits rice production. host-plant resistance is one of the most efficient ways to overcome bph damage to the rice crop. | 2017 | 28861736 |
| imidacloprid-susceptible nilaparvata lugens individuals exceeded resistant individuals in a mixture population with density pressure. | fitness costs associated with insecticide resistance in pest insects have mainly been studied under optimal laboratory conditions. however, resistant insects face more stressors than just insecticides in the field, and how the resistant population reacts to these stressors is of practical importance for the control of pest insects such as the brown planthopper nilaparvata lugens. the aim of the present study was to explore the impact of population density on the competitiveness of resistant and ... | 2018 | 28834145 |
| mesoionic pyrido[1,2-a]pyrimidinone insecticides: from discovery to triflumezopyrim and dicloromezotiaz. | one of the greatest global challenges is to feed the ever-increasing world population. the agrochemical tools growers currently utilize are also under continuous pressure, due to a number of factors that contribute to the loss of existing products. mesoionic pyrido[1,2-a]pyrimidinones are an unusual yet very intriguing class of compounds. known for several decades, this class of compounds had not been systemically studied until we started our insecticide discovery program. this account provides ... | 2017 | 28825462 |
| identification and analysis of brown planthopper-responsive micrornas in resistant and susceptible rice plants. | the brown planthopper (bph) is the most devastating insect pest of rice. the rice gene bph15 confers resistance to bph. micrornas (mirnas) regulate a spectrum of development and defense response processes in plants. in this study, we analyzed six mirna profiles of a bph15 introgression line (p15) and a susceptible recipient line (pc) at three time points (0 h, 6 h and 48 h) after bph attack, and identified 464 known mirnas and 183 potential novel mirnas. before the bph feeding, we identified 23 ... | 2017 | 28821824 |
| molecular structure of a prostaglandin d synthase requiring glutathione from the brown planthopper, nilaparvata lugens. | prostaglandins are involved in many physiological processes, and prostaglandin synthases facilitate the detoxification of xenobiotics as well as endogenous compounds, such as through glutathione conjugation. specifically, prostaglandin d synthase (pgds) catalyzes the isomerization of pgh2 to pgd2. here we report the identification and structural analysis of pgds from the brown planthopper rice pest nilaparvata lugens (nlpgds), which belongs to the sigma-class glutathione transferases. the struct ... | 2017 | 28803983 |
| development of 25 near-isogenic lines (nils) with ten bph resistance genes in rice (oryza sativa l.): production, resistance spectrum, and molecular analysis. | a first set of 25 nils carrying ten bph resistance genes and their pyramids was developed in the background of indica variety ir24 for insect resistance breeding in rice. brown planthopper (nilaparvata lugens stal.) is one of the most destructive insect pests in rice. development of near-isogenic lines (nils) is an important strategy for genetic analysis of brown planthopper (bph) resistance (r) genes and their deployment against diverse bph populations. a set of 25 nils with 9 single r genes an ... | 2017 | 28795219 |
| co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and rna silencing suppressor unilaterally. | synergism between southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) not only aggravates disease symptoms but also enhances their vector acquisition efficiencies by increasing both viruses' titers in co-infected rice plants, which may exacerbate the epidemic of both viruses and cause significant damage to rice production. the molecular mechanism of viral synergism of these two viruses remains unexplored. | 2017 | 28789694 |
| two distinctive β subunits are separately involved in two binding sites of imidacloprid with different affinities in locusta migratoria manilensis. | due to great diversity of nicotinic acetylcholine receptor (nachr) subtypes in insects, one β subunit may be contained in numerous nachr subtypes. in the locust locusta migratoria, a model insect species with agricultural importance, the third β subunits (locβ3) was identified in this study, which reveals at least three β subunits in this insect species. imidacloprid was found to bind nachrs in l. migratoria central nervous system at two sites with different affinities, with kd values of 0.16 an ... | 2017 | 28755692 |
| elevenin regulates the body color through a g protein-coupled receptor nla42 in the brown planthopper nilaparvata lugens. | the neuropeptide elevenin and similar neuropeptide precursors are common in some invertebrates but their physiological function in most species has not been explored. the brown planthopper, nilaparvata lugens (stål) has an elevenin-like peptide and a g protein-coupled receptor (gpcr) nla42 that is homologous to the elevenin receptor of the annelid platynereis dumerilii. rna interference (rnai)-mediated knockdown of either nl-elevenin or the nla42 gene resulted in cuticle melanization. ion transp ... | 2017 | 28743555 |
| effects of transgenic rice infected with srbsdv on bt expression and the ecological fitness of non-vector brown planthopper nilaparvata lugens. | the susceptibility of rice lines, t1c-19, t2a-1, and mh63 to srbsdv infection are similar and the contents of cry protein in t2a-1 and t1c-19 do not change significantly. the survival rates of bph nymphs feeding on srbsdv-infected t1c-19, bt t2a-1, or mh63 rice plants were not significantly different. the developmental stages of female bph fed on t1c-19 plants infected with srbsdv were significantly shorter than those fed on uninfected rice, while the males showed no significant difference. the ... | 2017 | 28740253 |
| the c-jun n-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication. | no evidence has shown whether insect-borne viruses manipulate the c-jun n-terminal kinase (jnk) signaling pathway of vector insects. using a system comprising the plant virus rice stripe virus (rsv) and its vector insect, the small brown planthopper, we have studied the response of the vector insect's jnk pathway to plant virus infection. we found that rsv increased the level of tumor necrosis factor-α and decreased the level of g protein pathway suppressor 2 (gps2) in the insect vector. the vir ... | 2017 | 28716183 |
| higher fertilizer inputs increase fitness traits of brown planthopper in rice. | rice (oryza sativa l.) is the primary staple food source for more than half of the world's population. in many developing countries, increased use of fertilizers is a response to increase demand for rice. in this study, we investigated the effects of three principal fertilizer components (nitrogen, phosphorus and potassium) on the development of potted rice plants and their effects on fitness traits of the brown planthopper (bph) [nilaparvata lugens (stål) (homoptera: delphacidae)], which is a m ... | 2017 | 28680158 |
| silencing osslr1 enhances the resistance of rice to the brown planthopper nilaparvata lugens. | della proteins, negative regulators of the gibberellin (ga) pathway, play important roles in plant growth, development and pathogen resistance by regulating multiple phytohormone signals. yet, whether and how they regulate plant herbivore resistance remain unknown. we found that the expression of the rice della gene osslr1 was down-regulated by an infestation of female adults of the brown planthopper (bph) nilaparvata lugens. on one hand, osslr1 positively regulated bph-induced levels of two mit ... | 2017 | 28666057 |
| mechanisms of callose deposition in rice regulated by exogenous abscisic acid and its involvement in rice resistance to nilaparvata lugens stål (hemiptera: delphacidae). | callose is a plant cell wall polysaccharide controlled by β-1,3-glucanase and synthase. abscisic acid (aba) is an important plant hormone. exogenous aba promotes rice resistance to pests. whether exogenous aba could reduce the decline in rice yield after brown planthopper (nilaparvata lugens stål; bph) feeding is an important question, however, the mechanisms behind rice resistance induced by aba remain obscure. | 2017 | 28664567 |
| karmoisin and cardinal ortholog genes participate in the ommochrome synthesis of nilaparvata lugens (hemiptera: delphacidae). | ommochrome is the major source for eye coloration of all insect species so far examined. phenoxazinone synthetase (phs) has always been regarded as the terminal step enzyme for ommochrome formation, which is encoded by cardinal or karmoisin genes. our previous study indicated that the karmoisin ortholog gene (nl-karmoisin) product in the brown planthopper (bph) was a monocarboxylate transporter, while not a phs. here, based on full-length complementary dna, the cardinal ortholog gene in bph (nl- ... | 2017 | 28657684 |
| silicon amendment is involved in the induction of plant defense responses to a phloem feeder. | plant resistance to herbivores is a key component in integrated pest management. in most cases, silicon (si) amendment to plants enhances resistance to herbivorous insects. the increase of plant physical barrier and altered insect behaviors are proposed as mechanisms for the enhanced resistance in si-amended plants, but our understanding of the induced mechanisms involved in si-enhanced plant resistance to phloem-feeding insects remains unclear. here, we show that si amendment to rice (oryza sat ... | 2017 | 28652621 |
| interaction of ferulic acid with glutathione s-transferase and carboxylesterase genes in the brown planthopper, nilaparvata lugens. | plant phenolics are crucial defense phytochemicals against herbivores and glutathione s-transferase (gst) and carboxylesterase (care) in herbivorous insects are well-known detoxification enzymes for such xenobiotics. to understand relationship between a plant phenolic and herbivore gst or care genes, we evaluated the relationship between a rice phenolic ferulic acid and resistance to brown planthopper (bph, nilaparvata lugens), and investigated the interaction of ferulic acid with gst or care ge ... | 2017 | 28647840 |
| development of elite bph-resistant wide-spectrum restorer lines for three and two line hybrid rice. | hybrid rice has contributed significantly to the world food security. breeding of elite high-yield, strong-resistant broad-spectrum restorer line is an important strategy for hybrid rice in commercial breeding programs. here, we developed three elite brown planthopper (bph)-resistant wide-spectrum restorer lines by pyramiding big-panicle gene gn8.1, bph-resistant genes bph6 and bph9, fertility restorer genes rf3, rf4, rf5, and rf6 through molecular marker assisted selection. resistance analysis ... | 2017 | 28638401 |
| knockdown of tor causing ovarian diapause in a genetically stable brachypterous strain of nilaparvata lugens. | brown planthopper (bph), nilaparvata lugens (stål) (hemiptera: delphacidae), is one of the most damaging pests of rice crops. bph is a migratory insect with a delayed ovarian development in migrants classified as reproductive diapause. the molecular mechanism of reproductive diapause remains unclear, although we suspect it might be regulated by one or more nutrient signaling pathways. the target of rapamycin (tor) pathway regulates cell growth in response to nutritional information, which raised ... | 2017 | 28635136 |
| pharmacological characterisation and functional roles for egg-laying of a β-adrenergic-like octopamine receptor in the brown planthopper nilaparvata lugens. | octopamine, the invertebrate counterpart of adrenaline and noradrenaline, controls and modulates many physiological and behavioral processes in protostomes. it mediates its effects by binding to specific receptors belonging to the superfamily of g-protein coupled receptors. we report the cloning of a cdna from the brown planthopper (nloa2b2) sharing high similarity with members of the oa2b2 receptor class. activation of nloa2b2 by octopamine increased the production of camp in a dose-dependent m ... | 2017 | 28629966 |
| forkhead box transcription factor l2 activates fcp3c to regulate insect chorion formation. | most animals are oviparous. however, the genes regulating egg shell formation remain not very clear. in this study, we found that nilaparvata lugens forkhead box transcription factor l2 (nlfoxl2) directly activated follicle cell protein 3c (nlfcp3c) to regulate chorion formation. nlfoxl2 and nlfcp3c had a similar expression pattern, both highly expressed in the follicular cells of female adults. knockdown of nlfoxl2 or nlfcp3c also resulted in the same phenotypes: obesity and female infertility. ... | 2017 | 28615473 |
| jinggangmycin-suppressed reproduction in the small brown planthopper (sbph), laodelphax striatellus (fallen), is mediated by glucose dehydrogenase (gdh). | the small brown planthopper (sbph), laodelphax striatellus (fallen), is a serious pest insect of rice, wheat, and maize in china. sbph not only sucks plant sap but also transmits plant disease viruses, causing serious damage. these viruses include rice striped virus disease (rsv disease), black streaked dwarf, and maize rough disease virus. sbph outbreaks are related to the overuse of pesticides in china. some pesticides, such as triazophos, stimulate the reproduction of sbph, but an antibiotic ... | 2017 | 28595925 |
| quantitative analysis of rice stripe virus in a transovarial transmission cycle during the development and reproduction of its vector, laodelphax striatellus. | the amount of rice stripe virus (rsv) maintained through transovarial transmission was analyzed during the development and reproduction of its vector, laodelphax striatellus. reverse transcription quantitative pcr analysis was used to quantify rna expressed from the rsv coat protein (cp) gene as an estimate of rsv content in nymphs and adults of l. striatellus at various developmental stages. the 18s ribosome rna gene of l. striatellus was chosen as the reference for calculating rsv cp expressio ... | 2017 | 28589385 |
| design, synthesis, and biological activities of novel pyrazole oxime compounds containing a substituted pyridyl moiety. | in this paper, in order to find novel biologically active pyrazole oximes, a series of pyrazole oxime compounds bearing a substituted pyridyl unit were prepared. bioassays showed that some target compounds were found to have good acaricidal activity against tetranychus cinnabarinus at a concentration of 500 μg/ml, compound 9q especially displayed potent acaricidal activity against t. cinnabarinus when the concentration was reduced to 100 μg/ml. interestingly, most target compounds possessed exce ... | 2017 | 28587083 |
| cycle affects imidacloprid efficiency by mediating cytochrome p450 expression in the brown planthopper nilaparvata lugens. | circadian clocks influence most behaviours and physiological activities in animals, including daily fluctuations in metabolism. however, how the clock gene cycle influences insects' responses to pesticides has rarely been reported. here, we provide evidence that cycle affects imidacloprid efficacy by mediating the expression of cytochrome p450 genes in the brown planthopper (bph) nilaparvata lugens, a serious insect pest of rice. survival bioassays showed that the susceptibility of bph adults to ... | 2017 | 28543984 |
| genomics of interaction between the brown planthopper and rice. | rice (oryza sativa l.) and the brown planthopper (nilaparvata lugens (stål)) form a model system for dissection of the mechanism of interaction between insect pest and crop. in this review, we focus on the genomics of bph-rice interaction. on the side of rice, a number of bph-resistance genes have been identified genetically. thirteen of these genes have been cloned which shed a light on the molecular basis of the interaction. on the aspect of bph, a lot of salivary proteins have been identified ... | 2017 | 28521948 |
| transgenic bt rice lines producing cry1ac, cry2aa or cry1ca have no detrimental effects on brown planthopper and pond wolf spider. | transgenic rice expressing cry genes from the bacterium bacillus thuringiensis (bt rice) is highly resistant to lepidopteran pests. the brown planthopper (bph, nilaparvata lugens) is the main non-target sap-sucking insect pest of bt transgenic rice. the pond wolf spider (pws, pardosa pseudoannulata) is one of the most dominant predators of bph in rice fields. consequently, the safety evaluation of bt rice on bph and pws should be conducted before commercialization. in the current study, two expe ... | 2017 | 28512299 |
| bacterial microbiota in small brown planthopper populations with different rice viruses. | the small brown planthopper (sbph) is an important virus vector, transmitting rice stripe virus (rsv), and rice black-streaked dwarf virus (rbsdv). insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by sbph. here, we investigated endosymbiont communities in non-viruliferous, rsv-infected, and rbsdv-infected sbph populations using illumina 16s rrna gene miseq sequencing. in total, 281,803 effective sequences ... | 2017 | 28471518 |
| development of a simplified rt-pcr without rna isolation for rapid detection of rna viruses in a single small brown planthopper (laodelphax striatellus fallén). | the small brown planthopper (sbph) is an important pest of cereal crops and acts as a transmission vector for multiple rna viruses. rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. reverse transcription polymerase chain reaction (rt-pcr) is a rapid, sensitive and reliable method for virus detection. the traditional rt-pcr contains a rna isolation step and is widely used for virus detection in insect. however, using the traditional rt-pcr for ... | 2017 | 28468626 |
| identification of snps involved in regulating a novel alternative transcript of p450 cyp6er1 in the brown planthopper. | cytochrome p450-mediated metabolic resistance is one of the major mechanisms involved in insecticide resistance. although the up-regulation of cytochrome p450 plays a vital role in insecticide metabolism, the molecular basis for the transcriptional regulation of cytochrome p450 remains largely unknown. the p450 gene cyp6er1, has been reported to confer imidacloprid resistance to the brown planthopper, nilaparvata lugens. here, we identified a novel alternative transcript of cyp6er1 (transcript a ... | 2017 | 28459131 |
| silicon amendment to rice plants impairs sucking behaviors and population growth in the phloem feeder nilaparvata lugens (hemiptera: delphacidae). | the brown planthopper (bph), nilaparvata lugens (stål), is a migratory and destructive sucking insect pest of rice. silicon (si) amendment to plants can confer enhanced resistance to herbivores and is emerging as a novel approach for pest management. in the present study, we tested the effects of si addition at 0.16 (low) and 0.32 (high) g si/kg soil on sucking behaviors and population growth in bph. si amendment increased si content in rice stems and extended non-probing event and phloem punctu ... | 2017 | 28439066 |
| point mutations in acetylcholinesterase 1 associated with chlorpyrifos resistance in the brown planthopper, nilaparvata lugens stål. | insecticide resistance frequently results from target-site insensitivity, such as point mutations in acetylcholinesterases (aches) for resistance to organophosphates and carbamates. from a field-originated population of nilaparvata lugens, a major rice pest, a resistant population (r9) was obtained by nine-generation continuous selection with chlorpyrifos. from the same field population, a relatively susceptible population (s9) was also constructed through rearing without any insecticides. compa ... | 2017 | 28407384 |
| brown planthopper nilaparvata lugens was concentrated at the rear of the typhoon soudelor in eastern china in august 2015. | sometimes, extreme weather is vital for the population survival of migratory insects by causing sudden population collapse or outbreak. several studies have shown that rice planthopper migration was significantly influenced by typhoons in eastern asia. most typhoons occur in the summer, especially in august. in august, brown planthopper nilaparvata lugens (stål) migrates northward or southward depending on wind direction, and thus typhoons can potentially influence its migration process and popu ... | 2017 | 28371321 |
| use of banker plant system for sustainable management of the most important insect pest in rice fields in china. | to meet the world's food demand, there is a growing need for sustainable pest management practices. this study describes the results from complementary laboratory and field studies of a "banker plant system" for sustainable management of the rice brown planthopper (bph) (nilaparvata lugens stål) - the economically most important rice pest in asian rice growing areas. the banker plant system consisted of planting a grass species, leersia sayanuka, adjacent to rice fields. l. sayanuka is the host ... | 2017 | 28367978 |
| glycogen phosphorylase and glycogen synthase: gene cloning and expression analysis reveal their role in trehalose metabolism in the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae). | rna interference has been used to study insects' gene function and regulation. glycogen synthase (gs) and glycogen phosphorylase (gp) are two key enzymes in carbohydrates' conversion in insects. glycogen content and gp and gs gene expression in several tissues and developmental stages of the brown planthopper nilaparvata lugens stål (hemiptera: delphacidae) were analyzed in the present study, using quantitative reverse-transcription polymerase chain reaction to determine their response to double ... | 2017 | 28365765 |
| suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, nilaparvata lugens. | trehalase (tre) is a key enzyme in trehalose degradation and has important functions in insect growth and chitin synthesis. though validamycin has the potential for pest control by suppressing tre activities, it is not known whether validamycin acts on both trehalose and chitin metabolism. tre1 and tre2 activities and glucose and glycogen contents decreased significantly after the injection of different doses of validamycin solution compared with the control group, while the trehalose content in ... | 2017 | 28364808 |
| the regulation of lipid metabolism by a hypothetical p-loop ntpase and its impact on fecundity of the brown planthopper. | insect fecundity can be regulated by multiple genes in several important signaling pathways which form an extremely complicated regulatory network. however, there are still many genes that have significant impact on insect fecundity but their action mode are still unknown. | 2017 | 28315769 |
| multiple atp-binding cassette transporters are involved in insecticide resistance in the small brown planthopper, laodelphax striatellus. | atp-binding cassette (abc) transporters are membrane-bound proteins involved in the movement of various substrates, including drugs and insecticides, across the lipid membrane. demonstration of the role of human abc transporters in multidrug resistance has led to speculation that they might be an important mechanism controlling the fate of insecticides in insects. however, the role of abc transporters in insects remains largely unknown. the small brown planthopper, laodelphax striatellus fallén, ... | 2017 | 28299835 |
| dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants. | brown planthopper (nilaparvata lugens stål, bph) causes huge economic losses in rice-growing regions and new strategies for combating bph are required. to understand how bphs respond towards bph-resistant plants, we systematically analyzed the metabolic differences between bphs feeding on the resistant and susceptible plants using nmr and gc-fid/ms. we also measured the expression of some related genes involving glycolysis and biosyntheses of trehalose, amino acids, chitin and fatty acids using ... | 2017 | 28278368 |
| distinct roles of met and interacting proteins on the expressions of takeout family genes in brown planthopper. | the takeout family genes encode relatively small proteins that are related to olfaction and are regulated by juvenile hormone (jh). the takeout genes modulate various physiological processes, such as behavioral plasticity in the migratory locust locusta migraloria and feeding and courtship behaviors in drosophila. therefore, to understand the regulatory mechanism of these physiological processes, it is important to study the expressions of the takeout genes that are regulated by jh signaling. we ... | 2017 | 28270774 |
| identification of two transcription factors activating the expression of osxip in rice defence response. | xylanase inhibitors have been confirmed to be involved in plant defence. osxip is a xip-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown. | 2017 | 28270131 |
| knockdown of a putative argininosuccinate lyase gene reduces arginine content and impairs nymphal development in nilaparvata lugens. | nilaparvata lugens is a typical phloem feeder. rice phloem is high in simple sugars and very low in essential amino acids. nilaparvata lugens harbors an ascomycete entomomyces delphacidicola that hypothetically biosynthesizes several amino acids to meet the nutrition requirement of the planthopper. among these amino acids, here, we focused on arginine biosynthesis. a complete cdna of an e. delphacidicola gene, arginine-succinate lyase, edarg4, the last step in arginine biosynthesis, was obtained ... | 2017 | 28251694 |
| gene expression and plant hormone levels in two contrasting rice genotypes responding to brown planthopper infestation. | the brown planthopper (bph; nilaparvata lugens stål) is a destructive piercing-sucking insect pest of rice. the plant hormones salicylic acid (sa) and jasmonic acid (ja) play important roles in plant-pest interactions. many isolated rice genes that modulate bph resistance are involved in the metabolism or signaling pathways of sa, ja and ethylene. 'rathu heenati' (rh) is a rice cultivar with a high-level, broad-spectrum resistance to all bph biotypes. here, rh was used as the research material, ... | 2017 | 28245796 |
| ras-like family small gtpases genes in nilaparvata lugens: identification, phylogenetic analysis, gene expression and function in nymphal development. | twenty-nine cdnas encoding ras-like family small gtpases (rsgs) were cloned and sequenced from nilaparvata lugens. twenty-eight proteins are described here: 3 from rho, 2 from ras, 9 from arf and 14 from rabs. these rsgs from n.lugens have five conserved g-loop motifs and displayed a higher degree of sequence conservation with orthologues from insects. rt-qpcr analysis revealed nlrsgs expressed at all life stages and the highest expression was observed in hemolymph, gut or wing for most of nlrsg ... | 2017 | 28241066 |
| comparative analysis of the transcriptional responses to low and high temperatures in three rice planthopper species. | the brown planthopper (nilaparvata lugens, bph), white-backed planthopper (sogatella furcifera, wbph) and small brown planthopper (laodelphax striatellus, sbph) are important rice pests in asia. these three species differ in thermal tolerance and exhibit quite different migration and overwintering strategies. to understand the underlying mechanisms, we sequenced and compared the transcriptome of the three species under different temperature treatments. we found that metabolism-, exoskeleton- and ... | 2017 | 28214356 |
| video monitoring of brown planthopper predation in rice shows flaws of sentinel methods. | immobilized preys are routinely used in agro-ecological exposure studies to quantify predation of pests under field conditions, but this method has not been validated. our purpose was to determine the validity of using immobilized adults of the major rice pest nilaparvata lugens, brown plant hopper (bph), as sentinels. we used direct observation by video recording to determine the causal agents of removal of field exposed bph sentinels with two experiments: 1) we recorded removal events of dead, ... | 2017 | 28211500 |
| the tiered-evaluation of the effects of transgenic cry1c rice on cyrtorhinus lividipennis, a main predator of nilaparvata lugens. | t1c-19, a newly developed transgenic cry1c rice line, expresses cry1c under the control of the maize ubiquitin promoter, and is highly resistant to lepidopteran pests of rice. cyrtorhinus lividipennis is the major predator of the eggs and young nymphs of nilaparvata lugens, which is the main non-target sap-sucking insect pest of bt rice. c. lividipennis may be exposed to cry1c protein, thus biosafety evaluations of transgenic cry1c rice on c. lividipennis should be conducted before the commercia ... | 2017 | 28205641 |
| adaptability of small brown planthopper to four rice cultivars using life table and population projection method. | in this study, we evaluated the adaptability of the small brown planthopper (sbph), laodelphax striatellus (hemiptera: delphacidae) to four rice cultivars including shengdao13 (sd13), shengdao14 (sd14), shengdao15 (sd15), and zixiangnuo (zxn) using the age-stage, two-sex life table with a simplified method for recording egg production (i.e., every five days vs. daily). the intrinsic rate of increase (r) of the sbph was the highest (0.1067 d-1) on cultivar sd15, which was similar to the rate on s ... | 2017 | 28205522 |
| reference genes for quantitative real-time pcr analysis in symbiont entomomyces delphacidicola of nilaparvata lugens (stål). | nilaparvata lugens (stål) (hemiptera: delphacidae) is a major rice pest that harbors an endosymbiont ascomycete fungus, entomomyces delphacidicola str. nlu (also known as yeast-like symbiont, yls). driving by demand of novel population management tactics (e.g. rnai), the importance of yls has been studied and revealed, which greatly boosts the interest of molecular level studies related to yls. the current study focuses on reference genes for rt-qpcr studies related to yls. eight previously unre ... | 2017 | 28198810 |
| evolution of rice stripe virus. | rice stripe virus (rsv) is an insect-borne tenuivirus of economical significance. it is endemic to the rice-growing regions of east asia and exhibits more genetic diversity in yunnan province of china. to gain more insights into the molecular epidemiology and evolution of rsv, recombination analyses were conducted and potential events were detected in each of the four rna segments of rsv. bayesian coalescent method was then applied to the time-stamped coding sequences of the cp gene. the nucleot ... | 2017 | 28189616 |
| effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant laodelphax striatella. | the resistance of the small brown planthopper (sbph), laodelphax striatella, to insecticides has been widely found in china, and has posed serious problems to efforts to control the pest. to determine the costs and benefits of resistance, the life tables of chlorpyrifos-resistant and -susceptible strains were constructed at 24 and 30°c. the results showed the resistant sbph (yn-cpf) had lower fitness at 24°c, but slightly higher fitness at 30°c compared to the susceptible sbph. transcriptomic an ... | 2017 | 28187833 |
| molecular characterization and expression pattern of three gaba receptor-like subunits in the small brown planthopper laodelphax striatellus (hemiptera: delphacidae). | ionotropic γ-aminobutyric acid (gaba)-gated chloride channel receptors mediate rapid inhibitory neurotransmission in vertebrates and invertebrates. gaba receptors are well known to be the molecular targets of synthetic insecticides or parasiticides. three gaba receptor-like subunits, lslcch3, lsgrd and ls8916, of the small brown planthopper, laodelphax striatellus (fallén), a major insect pest of crop systems in east asia, had been identified and characterized in this study. all three genes were ... | 2017 | 28187828 |
| metabolic resistance in nilaparvata lugens to etofenprox, a non-ester pyrethroid insecticide. | etofenprox, a non-ester pyrethroid insecticide, will be registered to control rice pests such as the brown planthopper (bph, nilaparvata lugens stål) in mainland china. insecticide resistance is always a problem to the effective control of n. lugens by chemical insecticides. an etofenprox resistance selection of n. lugens was performed in order to understand the related mechanisms. through successive selection by etofenprox for 16 generations in the laboratory, an etofenprox-resistant strain (g1 ... | 2017 | 28187826 |
| diversity-oriented synthesis of natural-product-like libraries containing a 3-methylbenzofuran moiety for the discovery of new chemical elicitors. | natural products are a major source of biological molecules. the 3-methylfuran scaffold is found in a variety of plant secondary metabolite chemical elicitors that confer host-plant resistance against insect pests. herein, the diversity-oriented synthesis of a natural-product-like library is reported, in which the 3-methylfuran core is fused in an angular attachment to six common natural product scaffolds-coumarin, chalcone, flavone, flavonol, isoflavone and isoquinolinone. the structural divers ... | 2017 | 28168155 |
| interactions between nitrogen and silicon in rice and their effects on resistance toward the brown planthopper nilaparvata lugens. | nitrogen (n) and silicon (si) are two important nutritional elements required for plant growth, and both impact host plant resistance toward insect herbivores. the interaction between the two elements may therefore play a significant role in determining host plant resistance. we investigated this interaction in rice (oryza sativa l.) and its effect on resistance to the herbivore brown planthopper nilaparvata lugens (bph). our results indicate that high-level (5.76 mm) n fertilization reduced si ... | 2017 | 28167952 |
| variation among conventional cultivars could be used as a criterion for environmental safety assessment of bt rice on nontarget arthropods. | the current difficulty facing risk evaluations of bacillus thuringiensis (bt) crops on nontarget arthropods (ntas) is the lack of criteria for determining what represents unacceptable risk. in this study, we investigated the biological parameters in the laboratory and field population abundance of nilaparvata lugens (hemiptera: delphacidae) on two bt rice lines and the non-bt parent, together with 14 other conventional rice cultivars. significant difference were found in nymphal duration and fec ... | 2017 | 28167821 |