Publications

protease and lipase activities of fungal and bacterial strains derived from an artisanal raw ewe's milk cheese.	we previously identified the microbiota present during cheese ripening and observed high protease and lipase activity in divle cave cheese. to determine the contribution of individual isolates to enzyme activities, we investigated a range of species representing this microbiota for their proteolytic and lipolytic ability. in total, 17 fungal, 5 yeast and 18 bacterial strains, previously isolated from divle cave cheese, were assessed. qualitative protease and lipase activities were performed on s ...	2016	27541978
generation and characterization of indoor fungal aerosols for inhalation studies.	in the indoor environment, people are exposed to several fungal species. evident dampness is associated with increased respiratory symptoms. to examine the immune responses associated with fungal exposure, mice are often exposed to a single species grown on an agar medium. the aim of this study was to develop an inhalation exposure system to be able to examine responses in mice exposed to mixed fungal species aerosolized from fungus-infested building materials. indoor airborne fungi were sampled ...	2016	26921421
penicillium salamii, a new species occurring during seasoning of dry-cured meat.	fungi have an important role in the production of dry-cured meat products, especially during the seasoning period. in general, both industrially and handmade salami are quickly colonized by a composite mycobiota during seasoning, often with a strong predominance of penicillium species. these species are involved in the improvement of the characteristics and taste, and in the prevention of the growth of pathogenic, toxigenic or spoilage fungi. during the survey of fungal species occurring on the ...	2015	25462928
an evaluation of the proteolytic and lipolytic potential of penicillium spp. isolated from traditional greek sausages in submerged fermentation.	a number of novel penicillium strains belonging to penicillium nalgiovense, penicillium solitum, penicillium commune, penicillium olsonii, and penicillium oxalicum species, isolated from the surface of traditional greek sausages, were evaluated for their proteolytic and lipolytic potential in a solid substrate first and next in submerged fermentations, using complex media. extracellular proteolytic activity was assessed at acid, neutral, and alkaline ph, while the lipolytic activity was assessed ...	2014	24122629
two new penicillium species penicillium buchwaldii and penicillium spathulatum, producing the anticancer compound asperphenamate.	penicillium buchwaldii sp. nov. (type strain cbs 117181(t) = ibt 6005(t) = imi 30428(t) ) and penicillium spathulatum sp. nov. (cbs 117192(t) = ibt 22220(t) ) are described as new species based on a polyphasic taxonomic approach. isolates of p. buchwaldii typically have terverticillate conidiophores with echinulate thick-walled conidia and produce the extrolites asperphenamate, citreoisocoumarin, communesin a and b, asperentin and 5'-hydroxy-asperentin. penicillium spathulatum is unique in ha ...	2013	23173673
factors affecting vegetable growers' exposure to fungal bioaerosols and airborne dust.	we have quantified vegetable growers' exposure to fungal bioaerosol components including (1→3)-β-d-glucan (β-glucan), total fungal spores, and culturable fungal units. furthermore, we have evaluated factors that might affect vegetable growers' exposure to fungal bioaerosols and airborne dust. investigated environments included greenhouses producing cucumbers and tomatoes, open fields producing cabbage, broccoli, and celery, and packing facilities. measurements were performed at different times d ...	2012	22003240
indoor mold concentration in eastern france.	our prospective case-control study of 118 dwellings in eastern france examined fungal contamination in unhealthy dwellings (n = 32) (homes with visible mold contamination and adverse health outcomes reported by the occupants), dwellings occupied by allergic patients (with medical diagnostic and positive prick-tests for molds) (n = 27) and matched control dwellings (n = 59). unhealthy dwellings present higher airborne concentrations of aspergillus, penicillium, and cladosporium than control dwell ...	2009	19840143
temperature downshift induces antioxidant response in fungi isolated from antarctica.	although investigators have been studying the cold-shock response in a variety of organisms for the last two decades or more, comparatively little is known about the difference between antioxidant cell response to cold stress in antarctic and temperate microorganisms. the change of environmental temperature, which is one of the most common stresses, could be crucial for their use in the biotechnological industry and in ecological research. we compared the effect of short-term temperature downshi ...	2009	19089529
moulds and ochratoxin a on surfaces of artisanal and industrial dry sausages.	the use of moulds as a seasoning for sausage can have both desirable and undesirable consequences. the desirable consequences are the creation of a successful product that appeals to consumers. the undesirable consequences are due to the growth of undesirable moulds that produce highly toxic secondary metabolites referred to as mycotoxins. the aim of the paper was to investigate the presence of moulds producing ochratoxin a (ota) on the surface of sausages from northern italy. a total of 757 mou ...	2009	19028307
biochemical and molecular characterization of a quercetinase from penicillium olsonii.	quercetinase (quercetin 2,3-dioxygenase, ec 1.13.11.24) is produced by various filamentous fungi when grown on rutin as the sole carbon and energy source. from a rutin based liquid culture of penicillium olsonii, we purified a quercetinase with a specific activity of 175u mg(-1). the enzyme is a monomeric glycoprotein of approximately 55 kda, containing 0.9+/-0.1 copper atoms per protein. its substrate specificity is restricted to the flavonol family of flavonoids. it is completely inhibited by ...	2008	18206655
evaluation of phenolics and sugars as inducers of quercetinase activity in penicillium olsonii.	quercetinase is produced by various filamentous fungi when grown on rutin as sole carbon and energy source. we investigated on the effect of 10 phenolics and two sugars, structurally related to substrates and products of the rutin catabolic pathway, on the induction of a quercetinase activity in penicillium olsonii. neither the sugars (glucose and rhamnose, two constituents of rutin), nor phenolics such as protocatechuic acid, salicylic acid, 4-hydroxy-benzoic acid and phloroglucinol were induce ...	2005	16288837
multilocus dna sequence analysis shows that penicillium biourgeianum is a distinct species closely related to p. brevicompactum and p. olsonii.	penicillium brevicompactum and other isolates with the compact, complex conidiogenous apparatus typical of the species were sequenced in one ribosomal and two protein coding regions. the aligned dna sequences were analyzed by maximum parsimony and the data from different loci were tested for compatibility using the partition homogeneity test. analysis of each of the three loci revealed three clades corresponding to p. brevicompactum, p. olsonii and p. biourgeianum. using the phylogenetic species ...	2004	15209283
sterol glycosides and cerebrosides accumulate in pichia pastoris, rhynchosporium secalis and other fungi under normal conditions or under heat shock and ethanol stress.	the occurrence of glycolipids such as sterol glycosides, acylated sterol glycosides, cerebrosides and glycosyldiacylglycerols was examined in the three yeast species candida albicans, pichia pastoris and pichia anomala, as well as in the six fungal species sordaria macrospora, pyrenophora teres, ustilago maydis, acremonium chrysogenum, penicillium olsonii and rhynchosporium secalis. cerebroside was found in all organisms tested, whereas acylated sterol glycosides and glycosyldiacylglycerols were ...	2001	11378896
cloning and targeted disruption of two polygalacturonase genes in penicillium olsonii.	the filamentous fungus penicillium olsonii secretes several polygalacturonases (pgs) with molecular masses of about 47 kda. these enzymes consist of several basic and acidic isoforms, with dominant activities at pi 4.5 and pi 7.9. two polygalacturonase genes, pg1 and pg2, have been cloned. the corresponding enzymes, pg1 and pg2, consist of 370 and 380 amino acids, respectively, and show significant similarities to endo-polygalacturonases from other filamentous fungi. targeted disruption of pg1 r ...	2000	10802187
isolation, structural identification and biological activity of two metabolites produced by penicillium olsonii bainier and sartory.	from the culture broth of a fungus, two metabolites have been isolated: bis(2-ethylhexyl)phthalate (dehp) precedently isolated from streptomyces sp. and 2-(4-hydroxyphenyl)-2-oxoacetaldehyde oxime (phba) here reported as a natural compound in the (e)-s-cis configuration. the producing organism was identified as a strain of penicillium olsonii. culture growth and chemical identification are discussed in the present work.	1994	8150716