Publications

molecular evolution and phylogenetic analysis of biocontrol genes acquired from scot polymorphism of mycoparasitic trichoderma koningii inhibiting phytopathogen rhizoctonia solani kuhn.	the biocontrol agent trichoderma (t. harzianum, t. viride, t. virens, t. hamantum, t. koningii, t. pseudokoningii and trichoderma species) inhibited variably (15.32 - 88.12%) the in vitro growth of rhizoctonia solani causing root rot in cotton. the t. koningii mtcc 796 evidenced highest (88.12%) growth inhibition of test pathogen followed by t. viride nbaii tv23 (85.34%). scanning electron microscopic study confirmed mycoparasitism for mtcc 796 and tv23 which were capable of completely overgrowi ...	2016	27720889
high-level expression and characterization of a novel serine protease in pichia pastoris by multi-copy integration.	a novel serine protease from trichoderma koningii (sptk) was synthesized and expressed in pichia pastoris. the recombinant sptk was completely inhibited by phenyl methyl sulfonyl fluoride (pmsf), suggesting that sptk belonged to the subgroup of serine proteases. the optimum ph and temperature for the recombinant sptk reaction were 6.0 and 55°c, respectively. sptk performed a tolerance to most organic solvents and metal ions, and the addition of triton x-100 exhibited an activation of sptk up to ...	2016	27542745
fungal biodeterioration, aflatoxin contamination, and nutrient value of "suya spices".	this work aimed to analyze the nutrient values, examine the biodeteriorating fungi biota, and analyze the mycotoxin contents of "suya spices." fungi with highest percentage occurrence on all the samples are aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus ochraceus, fusarium sp., rhizopus stolonifer, yeast, and trichoderma koningii. nutrient composition of the samples is significantly different statistically (p < 0.05) with high protein (9.53% to 13.17%), fiber (9.27 t ...	2016	27092289
chemical characterization of fatty acids, alkanes, n-diols and alkyl esters produced by a mixed culture of trichoderma koningii and penicillium janthinellum grown aerobically on undecanoic acid, potatoe dextrose and their mixture.	little is known about the mixed fungal synthesis of high-value aliphatics derived from the metabolism of simple and complex carbon substrates. trichoderma koningii and penicillium janthinellum were fed with undecanoic acid (uda), potatoe dextrose broth (pdb), and their mixture. pyrolysis field ionization mass spectrometry (py-fims) together with (1)h and (13)c nuclear magnetic resonance (nmr) characterized chcl3 soluble aliphatics in the fungal cell culture. data from nmr and py-fims analysis we ...	2016	26852878
hypocrol a, a new tyrosol derivative from a sponge-derived strain of the fungus hypocrea koningii.	in continuation of our search for new antibacterial and antioxidant metabolites from sponge-derived fungi, one new tyrosol derivative, hypocrol a (1), together with four known congeners, trichodenol b (2), 4-hydroxyphenethyl acetate (3), 4-hydroxyphenethyl tetradecanoate (4) and 1-oleyltyrosol (5), was isolated from the strain hypocrea koningii pf04. their planar structures were unequivocally elucidated by spectroscopic methods and comparison with the literature data. all the compounds displayed ...	2016	26828743
trichoderma species occurring on wood with decay symptoms in mountain forests in central europe: genetic and enzymatic characterization.	the aim of this study was to explore the species diversity of trichoderma obtained from samples of wood collected in the forests of the gorce mountains (location a), karkonosze mountains (location b) and tatra mountains (location c) in central europe and to examine the cellulolytic and xylanolytic activity of these species as an expression of their probable role in wood decay processes. the present study has led to the identification of the following species and species complex: trichoderma atro ...	2016	26586561
biodegradation of polycyclic aromatic hydrocarbons by trichoderma species: a mini review.	fungi belonging to trichoderma genus are ascomycetes found in soils worldwide. trichoderma has been studied in relation to diverse biotechnological applications and are known as successful colonizers of their common habitats. members of this genus have been well described as effective biocontrol organisms through the production of secondary metabolites with potential applications as new antibiotics. even though members of trichoderma are commonly used for the commercial production of lytic enzym ...	2015	26498812
new furan and cyclopentenone derivatives from the sponge-associated fungus hypocrea koningii pf04.	two new furan derivatives, hypofurans a and b (1 and 2), and three new cyclopentenone derivatives, hypocrenones a-c (3-5), along with seven known compounds (6-12), were isolated from a marine fungus hypocrea koningii pf04 associated with the sponge phakellia fusca. among them, compounds 10 and 11 were obtained for the first time as natural products. the planar structures of compounds 1-5 were elucidated by analysis of their spectroscopic data. meanwhile, the absolute configuration of 1 was deter ...	2015	26343687
metabolism of nc11 fatty acid fed to trichoderma koningii and penicillium janthinellum ii: production of intracellular and extracellular lipids.	little is known about the fungal metabolism of nc10 and nc11 fatty acids and their conversion into lipids. a mixed batch culture of soil fungi, t. koningii and p. janthinellum, was grown on undecanoic acid (uda), a mixture of uda and potato dextrose broth (uda+pdb), and pdb alone to examine their metabolic conversion during growth. we quantified seven intracellular and extracellular lipid classes using iatroscan thin-layer chromatography with flame ionization detection (tlc-fid). gas chromatogra ...	2014	25310811
metabolism of n-c10:0 and n-c11:0 fatty acids by trichoderma koningii, penicillium janthinellum and their mixed culture: i. biomass and co2 production, and allocation of intracellular lipids.	the capacity of two soil fungi, trichoderma koningii and penicillium janthinellum, to oxidize n-c10:0 and n-c11:0 fatty acids to co2 and store intracellular lipids during growth is unknown. this article reports for the first time the metabolism of decanoic acid (da, c10:0), undecanoic acid (uda, n-c11:0), a mixture of the acids (uda+da) and a mixture of uda+ potato dextrose broth (pdb) by t. koningii and p. janthinellum and their mixed culture. a control pdb complex substrate was used as a subst ...	2014	25310810
trichoderma songyi sp. nov., a new species associated with the pine mushroom (tricholoma matsutake).	a new species, trichoderma songyi, was found to be associated with the pine mushroom (tricholoma matsutake) in korea. this species was isolated from three different substrates: tricholoma matsutake basidiomata, as well as roots of pinus densiflora and soil in the fairy ring. based on its molecular and phenotypic characteristics, we demonstrate that trichoderma songyi is unique and distinguishable from closely related species. we performed phylogenetic analyses based on two molecular markers, the ...	2014	25052534
cellulose degradation and cellulase activity of five cellulolytic fungi.	biodegradation of pure cellulose powder, bagasse and wheatstraw by five cellulolytic fungi,aspergillus niger, chaetomium globosum, scopulariopsis brevicaulis, trichoderma koningii andtrichothecium roseum, was studied in solid culture conditions. minimum degradation was with pure cellulose. bagasse and wheatstraw were the most suitable for growth and activity of cellulolytic fungi. all fungi contained cellulase activity.	1990	24429892
bioconversion of straw into improved fodder: mycoprotein production and cellulolytic acivity of rice straw decomposing fungi.	sixty two out of the sixty four species of fungal isolates tested could produce both exo-β1,4-gluconase (c1) and endo-β1,4-gluconase (cx) on pure cellulose and rice straw as carbon source in czapek's medium. fifty-eight and fifteen species were able to grow at 25℃ and at 45℃, respectively. eleven species could grow at both 25℃ and 45℃ while, four species appeared only at 45℃. the most cellulolytic species at 25℃ was trichoderma koningii producing 1.164 c1 (mg glucose/1 ml culture filtrate/1 hr) ...	2005	24049480
bioconversion of straw into improved fodder: preliminary treatment of rice straw using mechanical, chemical and/or gamma irradiation.	crude protein (cp) content of mechanically ground rice straw into small particles by an electric grinder and reducing value (rv) and soluble protein (sp) in the culture filtrate were lower than that of the chopped straw into 5~6 cm lengths when both ground and chopped straws were fermented with aspergillus ochraceus, a. terreus or trichoderma koningii, at steady conditions. the reduction rate of rv, sp and cp was 22.2, 2.4, 7.3%; 9.1, 4.9, 8.5% or 0.0, 0.0, 3.6% for the three fungi, respectively ...	2006	24039464
cellulase production and saccharification of rice straw by the mutant strain hypocrea koningii rsc1.	the production of cellulase using solid-state fermentation of rice straw by the mutant strain hypocrea koningii rsc1 was studied. optimization of culture conditions, such as the nitrogen source, ph, and temperature, resulted in a maximum filter paper cellulase activity of 44.15 u g(-1) substrate, a carboxymethylcellulase activity of 324.6 u g(-1) substrate, and a β-glucosidase activity of 7.45 u g(-1) substrate. saccharification of untreated, 1% h(2)so(4)-treated, and 2.5% naoh-treated rice stra ...	2014	23775722
rna interference with carbon catabolite repression in trichoderma koningii for enhancing cellulase production.	the cellulase and xylanase genes of filamentous trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (crei). our objective was to find the role of crei in a cellulase-hyperproducing mutant of trichoderma koningii, and address whether enzyme production can be further improved by silencing the cre1 gene. cre1 partially silenced strains were constructed to improve enzyme production in t. koningii yc01, a cellulase-hyperproducing mutant. si ...	2013	23769310
establishment of an efficient rna silencing system in trichoderma koningii using dsred as a reporter.	we aimed to establish an efficient rna interference (rnai) system in the industrially important filamentous fungus trichoderma koningii using the dsred protein as a reporter of the silencing process. to accomplish this, a dsred expression cassette was transformed into t. koningii, and a recombinant strain that stably expressed dsred was obtained. next, a vector-directing expression of a dsred hairpin rna was constructed and transformed into the t. koningii recipient strain. approximately 79 % of ...	2013	23588496
effect of fungi and light on seed germination of three opuntia species from semiarid lands of central mexico.	fungal attack under light reduces mechanical resistance of the testa of opuntia seeds, making it easier for the embryo to emerge. however, the effect of fungi on opuntia seed germination in darkness is unknown. we evaluated the combined effects of light and inoculation with phoma medicaginis, trichoderma harzianum, trichoderma koningii, and penicillium chrysogenum on germination of o. streptacantha, o. leucotricha, and o. robusta seeds, from central mexico. we also evaluated the combined effects ...	2013	23526153
isolation, identification and antimicrobial activity of propolis-associated fungi.	propolis is a natural product widely known for its medicinal properties. in this work, fungi present on propolis samples were isolated, identified and tested for the production of antimicrobial metabolites. twenty-two fungal isolates were obtained, some of which were identified as alternaria alternata, aspergillus flavus, bipolaris hawaiiensis, fusarium merismoides, lasiodiplodia theobromae, penicillium citrinum, penicillium crustosum, penicillium janthinellum, penicillium purpurogenum, pestalot ...	2013	23439233
l-glutaminase production by trichoderma koningii under solid-state fermentation.	solid state fermentation was conducted for the production of l-glutaminase by trichoderma koningii oud.aggr. using different agro-industrial byproducts inlcuding wheat bran, groundnut residues, rice hulls, soya bean meal, corn steep, sesamum oil cake, cotton seed residues and lentil industrial residues as solid substrates. wheat bran was the best substrate for induction of l-glutaminase (12.1 u/mg protein) by t. koningii. the maximum productivity (23.2 u/mg protein) and yield (45.0 u/gds) of l-g ...	2009	23100777
improving cellulase production in trichoderma koningii through rna interference on ace1 gene expression.	ribonucleic acid interference (rnai) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. in this study, the function of the repressor of cellulase expression i (acei) was verified in trichoderma koningii (t. koningii) yc01 through rnai, and ace1- silenced strains with improved cellulase productivity were obtained. an expression cassette that transcrib ...	2012	22713991
construction of transgenic trichoderma koningi with chit42 of metarhizium anisopliae and analysis of its activity against the asian corn borer.	the chit42 gene was cloned from metarhizium anisopliae cy1 and was inserted into trichoderma koningii t30 genome by protoplast transformation. sixteen transgenic isolates were identified by polymerase chain reaction analysis. the chit42 gene was 1275 bp in length and its coded protein was approximately 42 kda in size. semi-quantitative rt-pcr analysis and the measurement of the chitinase activity under induced conditions were conducted. mortality of the asian corn borer (ostrinia furnacalis) was ...	2012	22560024
Evaluation of cellulases produced from four fungi cultured on furfural residues and microcrystalline cellulose.	Four fungal strains-Trichoderma viride, Aspergillus niger, Trichoderma koningii, and Trichoderma reesei-were selected for cellulase production using furfural residues and microcrystalline cellulose (MCC) as the substrates. The filter paper activity (FPA) of the supernatant from each fungus was measured, and the performance of the enzymes from different fungal strains was compared. Moreover, the individual activities of the three components of the cellulase system, i.e., ß-glucosidase, endoglucan ...	2011	22116409
[antagonism of trichoderma spp. to fungi caused root rot of sophora tonkinensis].	to study the antagonism of trichoderma spp. to fungi s9(fusarium solani)which caused root rot of sophora tonkinensis and discuss the further develop prospects of microbial biological control in soil-borne diseases on chinese herbal medicines.	2011	21809533
cunninghamella echinulata a new biosorbent of metal ions from polluted water in egypt.	thirty-two fungal species were isolated from a polluted watercourse near the talkha fertilizer plant, mansoura province, egypt. aspergillus niger, a. flavus, cunninghamella echinulata and trichoderma koningii were isolated frequently. on the basis of its frequency, cunninghamella echinulata was chosen for biosorption studies. free and immobilized biomass of c. echinulata sequestered ions in this decreasing sequence is: pb >cu >zn. the effects of biomass concentration, ph and time of contact were ...	2004	21148940
are fungi important for breaking seed dormancy in desert species? experimental evidence in opuntia streptacantha (cactaceae).	seeds of opuntia spp. have physiological dormancy; they need a period of after-ripening to break dormancy, and the embryos have low growth potential. we evaluated the combined effects of seed age and presence of fungi on the testa on germination of opuntia streptacantha, an abundant species in the chihuahuan desert (mexico), assuming that older seeds have broken seed dormancy and fungi can reduce mechanical resistance to germination. in a preliminary experiment, we found no germination of 9-year ...	2011	21143736
further evidence from the effect of fungi on breaking opuntia seed dormancy.	recently, we found that fungi are involved in breaking seed dormancy of opuntia streptacantha, and that the effect of fungi on seeds is species-specific. however, the effect of fungi on seed germination from other opuntia spp has not been evaluated. thus, we evaluated the effect of four fungal species (penicillium chrysogenum, phoma sp., trichoderma harzianum, trichoderma koningii) on the germination of opuntia leucotricha, an abundant species in the chihuahuan desert, mexico. we found that seed ...	2010	20861689
a method of purification, identification and characterization of β-glucosidase from trichoderma koningii as3.2774.	in this study, we used native gradient-polyacrylamide gel electrophoresis and electroelution (nggee) to purify enzymatic proteins from trichoderma koningii as3.2774. with this method, we purified eight enzymatic proteins and classified them to the cellulase system by comparing secretions of t. koningii in inductive medium and in repressive medium. it resulted in 24-fold β-glucosidase (bg) purification with a recovery rate of 5.5%, and a specific activity of 994.6iumg(-1) protein. the final yield ...	2010	20691221
trichodermaketones a-d and 7-o-methylkoninginin d from the marine fungus trichoderma koningii.	five new polyketide derivatives, 7-o-methylkoninginin d (1) and trichodermaketones a-d (2-5), together with four known compounds, koninginins a, d, e, and f, were isolated from the marine-derived fungus trichoderma koningii. trichodermaketones a (2) and b (3) are unprecedented polyketides with a bistetrafuran-containing tricyclic skeleton. the chemical structures and absolute configurations of compounds 1-5 were elucidated by comparing with literature data and extensive spectroscopic methods, in ...	2010	20384316
[study on conformation transitions of trichokonin vi, a peptaibol-like antimicrobial peptide, in different solvents by circular dichroism spectroscopy].	trichokonin vi, a peptaibol-like antimicrobial peptides isolated from the cultured substrates of trichoderma koningii smf2, has 20 amino acid residues. the conformational flexibility of trichokonin vi in organic solvents with different polarities, aqueous solvents and membrane mimic solvents was studied by circular dichroism spectroscopy. trichokonin vi takes on a typical alpha-helical structure in different organic solvents, but helicity decreases in aqueous solvent. the helical content increas ...	2010	20384145
[analysis of differential protein expression of remi mutant of trichoderma koningii].	on purpose of expounding the phenotype change of trichoderma mutant which produces cellulase, protein profiles of t. koningii cicc and its mutant tk-2r-1 were detected by 2-de. eight unique spots were found and four of them were identified and their functions by ms-tof-tof were connected with growth and metabolization of trichoderma spp. the research provides a new theoretical basis and methods for the construction of trichoderma mutant which is used to produce efficient cellulase.	2009	19306692
[cloning and functional analysis of two regulators involved in regulation of cellulase formation in trichoderma koningii].	acei and xyr1 are two regulators that potentially involve in regulation of cellulases and xylanases formation in trichoderma reesei, they compete for a binding site in the xyn1 (xylanase1-encoding) gene promoter. to further investigate the mechanism for the transcriptional regulation of cellulases, dna-binding domains of both acei and xyr1 in t. koningii were expressed from e. coli. they both showed bindings to the cbh1 promoter fragment (-304 bp to -18 bp) by electrophoresis mobility shift assa ...	2008	19149196
synthesis and in vitro evaluation of the antifungal activities of dihydropyrimidinones.	copper (ii) chloride in the absence of any solvent, efficiently catalyses the synthesis of dihydropyrimidinones (80-96% yields) by the biginelli reaction. six compounds were selected and examined their antifungal activities against the radial growth of three fungal species viz., trichoderma hammatum, trichoderma koningii and aspergillus niger.	2008	18977139
soil chemical and physical properties associated with suppression of take-all of wheat by trichoderma koningii.	abstract trichoderma koningii, originally isolated from a take-all-suppressive soil in western australia, has been shown to protect wheat against take-all disease and increase grain yield in field trials in australia, china, and the united states. however, within a region, the level of protection provided by t. koningii can dramatically vary between field sites. we evaluated suppression of take-all by this fungus in eight silt loams from the pacific northwest of the united states and the influen ...	1997	18945008
synergism of endoenzyme and exoenzyme on hydrolysis of soluble cellulose derivatives.	a kinetic model that represents the reaction of hydrolysis of water-soluble cellulose derivatives by a mixed endo- and exoenzyme system is proposed with the following assumptions: at an early stage of the reaction, endoenzymes split the substrate molecule in order to supply the newly formed nonreducing ends to exoenzymes until the molecular weight of the substrates reaches a low value; after that point, the reaction kinetics obeys only the rate equation of the reaction of the exoenzymes in which ...	1986	18555406
the trichoderma koningii aggregate species.	the morphological concept of trichoderma koningii is found to include several species that differ from each other in details of phenotype (including conidium morphology, growth rate) and biogeography. phylogenetic analysis utilizing partial sequences of the translation-elongation factor 1 alpha (tef1), as well as fragments of actin and calmodulin genes, indicate that phenotypic characters typical of t. koningii evolved independently in three well-separated main lineages. combined molecular and p ...	2006	18490990
[filamentous fungi preservation in distilled water.].	some methods for keeping the fungal culture collection have been used. however, the choice of either one on the basis that must ensure the cultural genetic stability and its phenotipic characteristics. in this work the preservation method in distilled water recognized in the literature as a single, economic and certain method that guarantie the survival of fungus cultures for long periods was used. 26 strains of generus and species: aspergillus niger, aspergillus candidus, fusarium sp., fusarium ...	1998	18473541
evaluation of the effects of chemical versus biological control on botrytis cinerea agent of gray mould disease of strawberry.	this study investigates on effects of four fungicide and six isolate from trichoderma and gliocladium on botrytis cinerea agent grey mold of strawberry under library and greenhouse condition. the effect of four fungicides i.e. benomyl, dichlofluanid, captan and triadimenol on b. cinerea was studied in the laboratory condition by method mixed poison to culture medium. it was shown that the fungicide including benomyl, triadimenol, dichlofluanid and captan were able to inhibit mycelial growth of b ...	2007	18396812
characterization of a thermostable and acidic-tolerable beta-glucanase from aerobic fungi trichoderma koningii zju-t.	an extreme thermostable and acidic tolerable beta-glucanase was isolated and characterized from aerobic fungi trichoderma koningii zju-t. the optimal reaction temperature and ph for the beta-glucanase were 100 degrees c and ph 2.0, respectively. the beta-glucanase showed increased stability at higher temperatures and lower ph values when compared to other beta-glucanases. the optimum conditions for the beta-glucanase stability were found to be ph 4.0 and 80 degrees c. even subjected to 100 degre ...	2007	18034703
efficient biodegradation of cyanide and ferrocyanide by na-alginate beads immobilized with fungal cells of trichoderma koningii.	cyanide or metal cyanide contaminations have become serious environmental and food-health problems. a fungal mutant of trichoderma koningii, tka8, constructed by restriction enzyme-mediated integration, has been verified to have a high cyanide degradation ability in our previous study. in this study, the mutant cells were entrapped in sodium-alginate (na-alginate) immobilization beads to degrade cyanide and ferrocyanide in a liquid mineral medium. the results showed that the fungus in immobiliza ...	2007	18026223
koninginins, phospholipase a2 inhibitors from endophytic fungus trichoderma koningii.	many isolated compounds from endophytic fungus have been useful to human beings, mainly those with medicinal applications and particularly those that can be used in inflammatory processes. trichoderma fungi produce substances known as koninginins that have great structural similarity to compounds like flavonoids and vitamin e, which are able to inhibit the phospholipase a(2) (pla(2)). in this work, koninginins a, e and f (kona, kone and konf, respectivamente) isolated from trichoderma koningii h ...	2008	17983638
diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates.	the diversity of fungi, bacteria, yeast, actinomycetes and protozoa were analysed in the gut and casts of eudrilus eugeniae, lampito mauritii, eisenia fetida and perionyx excavatus, both qualitatively and quantitatively as influenced by different feed substrates like clay loam soil, cowdung and pressmud. while actinomycetes (streptomyces albus, s. somaliensis, nocardia asteroides, n. caviae and saccharomonosporia) were not digested by any of these species of worms, protozoa (amoeba proteus, a. t ...	2007	17717992
solid-state fermentation for trichokonins production from trichoderma koningii smf2 and preparative purification of trichokonin vi by a simple protocol.	trichokonins are peptaibols produced by trichoderma koningii smf2. the main isoforms are trichokonin vi, trichokonin vii and trichokonin viii. the solid-state fermentation (ssf) was applied for the production of trichokonin vi. the fermentation factors, which included inoculum size, incubation temperature, initial moisture content and initial ph, were investigated and optimized by response surface methodology. the maximum trichokonin vi production (4.07mg/g dry substrate) was achieved by employi ...	2007	17669535
[mycoparasites effect on reproductive ability of sclerotinia sclerotiorum sclerotia.].	the ability to parasitise sclerotinia sclerotiorum and the effect on apothecia production was evaluated for the following antagonists: trichoderma harzianum; trichoderma koningii; gliocladium roseum and chaetomium globosum. plastic trays were filled with of steam-sterilized soil. each one of them was infested with sclerotia of s. sclerotiorum and the culture of the antagonists. the trays were kept in a greenhouse and after 30, 60 and 90 days, evaluations were made. the rates of carpogenic germin ...	1998	17655415
operating characteristics of solid-state fermentation bioreactor with air pressure pulsation.	the development of solid state fermentation (ssf) technology is very important to the production of cellulase and ultimately to the utilization of natural cellulose. however, inadequate dissipation of heat generated by biological activities has prevented solid state fermentation from large-scale applications. the paper deals with the development of a novel ssf bioreactor with air pressure pulsation. by developing a measurement and control system under virtual instrument (vi) concept, performance ...	2007	17476813
optimization of cellulase complex formulation for peashrub biomass hydrolysis.	to improve efficiency and reduce cost, solid state simultaneous saccharification and fermentation of peashrub woody biomass was investigated under anaerobic conditions at 50 degrees c, with a cellulase-inoculant mixture consisting of trichoderma koningii cellulase, aspergillus niger cellulase, and lactobacillus. experimental formulations were prepared according to uniform prescription design principles. by crude protein, crude fiber models constructed using multivariate regression in spss and so ...	2007	17347818
[fungus microbiota in air conditioners in intensive care units in teresina, piauí].	with the aim of identifying the fungus microbiota in air conditioners in intensive care units (icus) within public and private hospitals in teresina, piauí, solid material was collected from ten different icus. thirty-three species of moniliaceae and dematiaceae were isolated, which was the first report of these in piauí. high frequencies of aspergillus niger van tieghem (60%), aspergillus fumigatus fres (50%), trichoderma koningii oudem (50%) and aspergillus flavus link: fr. (40%) were recorded ...	2006	17308702
tolerance of antarctic soil fungi to hydrocarbons.	little is known about the effects of hydrocarbons and fuel oil on antarctic filamentous fungi in the terrestrial antarctic environment. growth of fungi and bacteria from soils around rothera research station (adelaide island, antarctic peninsula) was assessed in the presence of ten separate aromatic and aliphatic hydrocarbons [marine gas oil (mgo), dodecane, hexadecane, benzoic acid, p-hydroxybenzoic acid, toluene, phenol, biphenyl, naphthalene and m- and p-xylenes with ethylbenzene]. aromatic h ...	2007	17157897
degradation of cyanide by trichoderma mutants constructed by restriction enzyme mediated integration (remi).	remi technique was used to construct mutants with improved cyanide-degradation ability from biocontrol fungus trichoderma koningii strain t30. the plasmid pv2 transformation was confirmed by pcr and southern blot analysis. out of 21 transformants, 15 single-copied transformants (71.4%) were found. to compare enzyme activities of rhodanese and cyanide hydratase, t. atroviride t23, t. harzianum t21 and their transformants constructed by remi previously were also included. transformants tkb6 (0.173 ...	2007	17112721
mutagenesis and mechanistic study of a glycoside hydrolase family 54 alpha-l-arabinofuranosidase from trichoderma koningii.	a gh (glycoside hydrolase) family 54 alpha-l-arabinofuranosidase from trichoderma koningii g-39 (termed abf) was successfully expressed in pichia pastoris and purified to near homogeneity by cation-exchange chromatography. to determine the amino acid residues essential for the catalytic activity of abf, extensive mutagenesis of 24 conserved glutamate and aspartate residues was performed. among the mutants, d221n, e223q and d299n were found to decrease catalytic activity significantly. the kcat v ...	2007	17002602
rapid and selective isolation of beta-xylosidase through an activity-based chemical approach.	beta-xylosidase is a key enzyme in the xylanolytic system with a great potential in many biotechnological applications, especially in the food as well as the pulp and paper industries. we have developed a chemical approach for the rapid screening and isolation of beta-xylosidase. activity probe lcl-6x targeting beta-xylosidase was utilized in this study. it carries a beta-xylopyranosyl recognition head, a latent trapping device consisting of a 2-fluoromethylphenoxyl group, and a biotin reporter ...	2006	16892248
beneficial effect of saprobe and arbuscular mycorrhizal fungi on growth of eucalyptus globulus co-cultured with glycine max in soil contaminated with heavy metals.	the effects of saprobe and arbuscular mycorrhizal (am) fungi on growth, chlorophyll and n, p and k content of eucalyptus globulus labill. growing in soil contaminated by heavy metals in the presence or absence of glycine max were investigated. glomus mosseae and glomus deserticola increased dry weight, shoot length, total n, p and k concentration and the quantity of chlorophyll in e. globulus shoots. the protection of eucalyptus by am fungi against the action of the heavy metals was more evident ...	2007	16837125
broad-spectrum antimicrobial activity and high stability of trichokonins from trichoderma koningii smf2 against plant pathogens.	antimicrobial metabolites produced by trichoderma koningii smf2 exhibited antimicrobial activity against a range of gram-positive bacterial and fungal phytopathogens. purification of these metabolites was achieved using combinations of gel filtration and high-performance liquid chromatography. identified by liquid chromatography electrospray ionization tandem mass spectrometry, the active metabolites proved to be three known peptaibols: trichokonin vi, vii and viii. the trichokonins were stable ...	2006	16790027
reidentification of cellulolytic enzyme-producing trichoderma strains w-10 and g-39.	strain w-10, originally identified as trichoderma koningii, and its supposed mutant g-39, published for production and gene expression of cellulase and xylanase, demonstrated morphological characteristics distinct from those of t. koningii, respectively. to clarify the identification derived from morphological characteristics, several methods were used, including electrophoretic karyotyping, internal transcribed spacer (its) analysis of rdna, and polymerase chain reaction (pcr) fingerprinting us ...	2006	16788725
the effect of self-designed bifunctional rgd-containing fusion protein on the behavior of human keratinocytes and dermal fibroblasts.	in this study, self-designed bifunctional rgd-containing fusion protein (bfp) was grafted on the petri dish to evaluate its cytotoxicity and attachment efficiency on primary cultured keratinocytes and dermal fibroblasts. two lengths of the grgds sequences were separately fused to the n-terminus and c-terminus of the trichoderma koningii cellobiohydrolase i gene cellulose-binding domain, to serve as linking molecule between the cell and the substrate. the grafting procedure was no more labor-inte ...	2006	16649172
biochemical characterization of a beta-1,3-glucanase from trichoderma koningii induced by cell wall of rhizoctonia solani.	trichoderma species are readily isolated from brazilian cerrado soil by conventional methods and some of them were characterized as trichoderma koningii. the effect of carbon source on the production of beta-1,3-glucanases in the culture filtrates of a specific trichoderma koningii strain (all 13) was investigated. enzyme activity was detected in all carbon sources tested and only one band of beta-1,3-glucanase was detected in non-denaturing page. this enzyme was purified by sephacryl s-200 gel ...	2006	16450064
microbial transformation of silybin by trichoderma koningii.	microbial transformation of silybin a (1) and silybin b (2), the major hepatoprotective flavonolignan diastereomers from the fruits of silybum marianum, with the culture broth of trichoderma koningii gave two pairs of glucosylated derivatives. their structures were identified as silybin a 3-o-beta-d-glucopyranoside (3), silybin a 7-o-beta-d-glucopyranoside (4), silybin b 3-o-beta-d-glucopyranoside (5) and silybin b 7-o-beta-d-glucopyranoside (6) by spectroscopic methods.	2006	16309908
process kinetics of inoculation composting of municipal solid waste.	a method was used to improve the composting efficiency by seeding with inoculum a (a blend of bacillus azotofixams, bacillus megaterium and bacillus mucilaginosus), inoculum b (a blend of effective cellulolytic strains, i.e. trichoderma koningii, streptomyces cellulosae, and white-rot fungi), and inoculum c (a mixture of inoculum a and inoculum b). there were four runs: the control run (not inoculated), run a, run b and run c. during the runs, parameters such as temperature, o2, co2 and h2s emis ...	2005	15961224
mycodiversity on a dead stem of the giant hogweed--heracleum mantegazzianum sommer et levier.	during our investigations on microfungi in the ruhr area we were able to find the remarkable mycodiversity of different genera and species on dead stems of heracleum mantegazzianum, a neophyt for the european flora. altemaria alternata (fr.fr.) keissl., camarosporium spec., cladosporium herbarum (pers.) link, colletotrichum dematium (pers. ex fr.) gove, dactylella armandii yadv., dasyscyphus mollissimus (lasch.) dennis, dendryphion comosum wallr., endophragmia hyalosperma (corda) morgan-jones & ...	2004	15756828
laboratory study of inocula production for composting processes.	six ligno-cellulolytic fungi were tested regarding to examine their capability to grow on agricultural wastes and produce inocula for composting. two residues were used: pepper plant wastes and almond shell residues. results showed the latter as the most adequate substrate for growth of fungi tested. on the contrary, trichoderma koningii, as well as hlc1 and hlc3, both fungi isolated from almond shell wastes, were able to persist in pepper plant wastes. modifications of aeration and ph significa ...	2005	15607193
optimization of solid fermentation of cellulase from trichoderma koningii.	to exploit peashrub resources in ordos as fodders, it is very crucial to realize industrial production of cheap cellulase of high activity by optimizing culture technology, especially culture substrate. in this study, a new prescription experiment based on uniform design ideal was invented and successfully applied in the solid fermentation of trichoderma koningii f244, which was performed with two different temperature degrees. the activities of fpa, cotton lyase, cmcase and beta-glucosidase wer ...	2004	15559819
hydrolysis of fibrous cotton and reprecipitated cellulose by cellulolytic enzymes from soil micro-organisms.	1. the action of cell-free filtrates from trichoderma koningii was examined on undegraded cellulose in the form of cotton fibres, on degraded cellulose in the form of cellulose powder reprecipitated from phosphoric acid and on the soluble cellulose derivative cm-cellulose. 2. the cell-free filtrates compare favourably with intact cellulolytic micro-organisms in producing complete solubilization of undegraded as well as of degraded types of cellulose. enzymic solubilization of cotton fibres gives ...	1965	14335814
indications for the tracking of elevated nitrogen levels through the fungal route in a soil food web.	the objective of the present study was to determine the effects of elevated n in dead organic matter on the growth of fungi and to establish the consequences for the development of microbivores. therefore, three fungal species were cultured on scots pine litter differing in n content. the growth of the soil fungal species trichoderma koningii, penicillium glabrum and cladosporium cladosporioides was directly influenced by the n content (ranging from 1.25 to 2.19% n) of the substrate. for all thr ...	2003	12927496
genetic and metabolic diversity of trichoderma: a case study on south-east asian isolates.	we have used isolates of trichoderma spp. collected in south-east asia, including taiwan and western indonesia, to assess the genetic and metabolic diversity of endemic species of trichoderma. ninety-six strains were isolated in total, and identified at the species level by analysis of morphological and biochemical characters (biolog system), and by sequence analysis of their internal transcribed spacer regions 1 and 2 (its1 and 2) of the rdna cluster, using ex-type strains and taxonomically est ...	2003	12684020
[selection of beta-glucanase-producing trichoderma köningii t199 and its fermentation conditions].	a mutant strain t199 producing about 8 times as much beta-glucanase as its parent strain trichoderma köningii t3 was obtained by treatment with ultraviolet light and n-methyl-n-nitro-n-nitrosoguanidine. fermentation was conducted in 250 ml flask, each containing 30 ml of medium consisted of 5% corn cob powder, 3% wheat bran and 1.4% nitrogen source no. 10 ((nh4)2so4 10%, peptone 20%, yeast extract 15%). the optima culture conditions were as below: initial ph 5.0, 30 degrees c, shaking speed 280 ...	2001	12552835
determination of the stereochemistry of (-)-koninginin a by an x-ray analysis of its synthetic sample.	the absolute configuration of (-)-koninginin a [10-hexyl-11, 12-dioxatricyclo[7.2. 1.0<1 ,6>]dodecane-2,5-diol (1)], the antibiotic metabolite of trichoderma koningii and t. harzianum, was determined as 1s, 2r, 5s, 6s, 9s, 10s by an x-ray crystallographic analysis of its synthetic sample coupled with the established stereochemical outcome of sharpless asymmetric dihydroxylation used as the key reaction to prepare intermediate 4.	2002	12353648
soil saprotrophic microfungi associated with roots of calamagrostis epigeios on an abandoned deposit of toxic waste from smelter factory processing pyrite raw materials.	saprotrophic microfungi associated with roots of calamagrostis epigeios growing in industrial waste contaminated with high concentrations of heavy metals (manganese, iron, zinc) were isolated on three different media. the isolates were obtained from three plots colonized by plant cenoses, representing three different succession series of plant cover: initial, intermediate, and advanced. a total of 60 species of saprotrophic microfungi were found in the roots during three samplings. in addition, ...	2001	12032621
combined use of lc/ms and a biological test for rapid identification of marine mycotoxins produced by trichoderma koningii.	trichoderma koningii oudemans, a strain isolated from a shellfish farming area, was selected for its high frequency in samples and its ability to produce metabolites when cultured in natural seawater. combined use of lc/ms and a biological test on blowfly larvae allowed the characterization of four compounds after purification in only two steps (vlc and hplc). esi/ms, a powerful tool for rapid identification and sequence determination of peptides, confirmed that these compounds were peptide, alp ...	2002	11777568
[effect of enhanced uv-b radiation on chemical composition, fungal colonization and decomposition of spring wheat plant].	under uv-b radiation, the cho (soluble carbohydrate) concentration in leaves decreased significantly, while the concentrations of hcel (holocellulose) and protein in leaves and that of hcel in stems increased significantly. hcel concentration in roots did not change significantly. under uv-b radiation, the colonization rates of penicillium ochro-chloron and aspergillus niger on leaves and stems increased significantly during growing phase, those of trichoderma koningii and aureobasidium pullulan ...	2001	11757366
trichoderma koningii as a biomineralizing fungous agent of calcium oxalate crystals in typical argiudolls of the los padres lake natural reserve (buenos aires, argentina).	the aim of the present study, performed on typical argiudolls in a natural reserve with little or no anthropic impact, was to characterize the fungous biomineralizing process of calcium oxalate crystals in organic horizons of the soil. the chosen sites possessed different plant cover, identified as acacia woods and grassy meadows with particular micro environmental conditions that have differing effects in the process of biomineralization. the contribution of the plant material in the soil is a ...	2001	11572450
effective extraction and purification of beta-xylosidase from trichoderma koningii fermentation culture by aqueous two-phase partitioning.	effective extraction of protein from bulk medium is an important technique in bioresearch. in the present study, we describe an extracellular beta-xylosidase from the fermentation supernatant of trichoderma koningii g-39 that was successfully extracted and purified simultaneously in a single step by using an aqueous two-phase partitioning method. this two-phase system was prepared by dissolving suitable amount of poly(ethylene glycol) (peg) and sodium dihydrogenphosphate (nah(2)po(4)) in aqueous ...	2001	11166812
physiological aspects of fungi isolated from root nodules of faba bean (vicia faba l.).	the present study was made to isolate and assess some physiological characteristics of root nodule-colonizing fungi. during this study, 17 fungal species were isolated from root nodule samples taken from faba bean plants (vicia faba l.) collected from different sites at assiut area (egypt). the growth of faba bean plants in pots was significantly promoted by soil inoculation with most fungi. growth was checked in pots with inocula of cladosporium cladosporioides, fusarium moniliforme, f: oxyspor ...	2000	10772156
effective induction, purification and characterization of trichoderma koningii g-39 beta-xylosidase with high transferase activity.	a beta-xylosidase was induced and purified from the culture filtrate of trichoderma koningii g-39, grown in a medium containing 1% oat spelts xylan and 0.1% xylose. the presence of xylose unequivocally enhanced the induction of beta-xylosidase. the purified enzyme, which exhibited a significant alpha-arabinosidase activity, was obtained with high yield simply via ethanol precipitation and a single anion-exchange chromatography and was characterized as a monomeric glycoprotein with an estimated m ...	2000	10744957
mechanistic study of beta-xylosidase from trichoderma koningii g-39.	the catalytic mechanism of the beta-xylosidase purified from the culture filtrate of trichoderma koningii g-39 was investigated. by nmr spectroscopy, the stereochemistry of the enzyme catalyzing the hydrolysis of 2,4-dinitrophenyl and p-nitrophenyl-beta-d-xylosides was found unequivocally to involve retention of the anomeric configuration. based on the k(cat) values of a series of arylxylosides with leaving group pk(a)s in the range of 4-10, an extended bronsted plot was constructed with a slope ...	2000	10731699
biocontrol of rhizoctonia solani and pythium ultimum on capsicum by trichoderma koningii in potting medium.	two isolates of trichoderma koningii were evaluated for efficacy in control of damping-off diseases in seedlings of capsicum annuum grown in pasteurized potting medium in a glasshouse. a selected isolate of binucleate rhizoctonia and two fungicides were also included as standards for control of rhizoctonia solani and pythium ultimum var. sporangiiferum. both isolates of t. koningii reduced seedling death caused by r. solani in one of two experiments, and by p. u. sporangii-ferum in two of three ...	1999	10522381
the abfb gene encoding the major alpha-l-arabinofuranosidase of aspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted strain.	using a dna fragment containing the aspergillus niger abfb gene as a probe, the homologous aspergillus nidulans gene, designated abfb, has been cloned from a genomic library containing size-selected hindiii fragments. the nucleotide sequence of the a. nidulans abfb gene shows strong homology with the a. niger abfb, trichoderma reesei abf-1 and trichoderma koningii alpha-l-arabinofuranosidase/beta-xylosidase genes. regulation of abfb expression has been investigated in cultures induced with l-ara ...	1999	10217508
total synthesis and membrane modifying properties of the lipopeptaibol trikoningin kb ii and its analogues with acyl chains of different length at the n- and c-termini.	trikoningin kb ii, a ten-amino acid residue lipopeptaibol blocked at the n-terminus by the n-octanoyl group and at the c-terminus by the 1,2-amino alcohol l-leucinol, and extracted from the fungus trichoderma koningii, exhibits membrane-modifying properties. we have synthesized by solution-phase methods trikoningin kb ii and several analogues with acyl chains of different length at the n- and c-termini. permeability measurements showed that an appropriate length of the linear acyl chain is a mor ...	1999	10100125
bioaccumulation of mycotoxins by shellfish: contamination of mussels by metabolites of a trichoderma koningii strain isolated in the marine environment.	to determine whether toxic metabolites produced by fungi could cause shellfish toxicities, mussels were contaminated in laboratory conditions by sterile filtrates of a liquid culture of a strain of the fungus trichoderma koningii previously isolated from a shellfish, the cockle (cerastoderma edule). mussels were kept in aerated natural seawater and fed with a culture of the microalga isochrysis galbana, to which a filtrate of liquid fungal culture was added. mussels were exposed to contamination ...	1999	9920482
fungal microflora biodiversity as a function of pollution in oued sebou (morocco).	an inventory of the fungal microflora present in sediments collected at 11 sites along oued sebou has been established. the influence of some climatic parameters (temperature, rainfall) and of pollution on the composition of the fungal communities has been questioned. emericella nidulans, eurotium amstelodami, neosartotya fisheri var. glabrum (ascomycetes), dreschslera biseptata (dematiaceae), penicillium citrinum (mucedinaceae) and phoma sp. (sphaeropsidale) can be considered as strains sensiti ...	1997	9253167
studies on metabolites of mycoparasitic fungi. v. ion-spray ionization mass spectrometric analysis of trichokonin-ii, a peptaibol mixture obtained from the culture broth of trichoderma koningii.	the sequence of a peptide, trichokonin-ii (tk-ii), obtained from the culture broth of trichoderma koningii oudemans, was examined by ion-spray ionization mass spectrometry (isi-ms), including the collision-induced dissociation (cid) technique. tk-ii was concluded to be a mixture of three peptaibols, tk-iia, tk-iib, and tk-iic.	1996	8882456
sensitivity of fungi to nikkomycin z.	nikkomycin z inhibited colony radial extension (kr) and hyphal density in geotrichum candidum, trichoderma koningii, mucor plumbeus, and fusarium oxysporum, although the level of inhibition varied with the fungal species and was influenced by the concentration of nutrients in the culture medium. while g. candidum and m. plumbeus were sensitive to the inhibitory effects of nikkomycin z, t. koningii and f. oxysporum were relatively tolerant of concentrations of the antibiotic up to 100 mumol. nikk ...	1996	8634944
on an unstable antifungal metabolite from trichoderma koningii. isolation and structure elucidation of a new cyclopentenone derivative (3-dimethylamino-5-hydroxy-5-vinyl-2-cyclopenten-1-one).		1996	8621363
studies on metabolites of mycoparasitic fungi. iv. minor peptaibols of trichoderma koningii.	three minor peptaibols, trichokonins (tks)-ia, ib, and ix, were obtained from the culture broth of trichoderma koningii oudemans. primary structures of these peptaibols were elucidated by ion-spray ionization mass spectrometry (isi-ms) including the collision-induced dissociation (cid) technique together with two-dimensional nuclear overhauser enhancement spectroscopy (noesy).	1995	8536339
cloning of two isozymes of trichoderma koningii glyceraldehyde-3-phosphate dehydrogenase with different sensitivity to koningic acid.	koningic acid inhibits glyceraldehyde-3-phosphate dehydrogenase (gapdh) by binding to the sh group in the active center. the fungus trichoderma koningii, the producer of koningic acid, contains two gapdh isozymes (gapdhs i and ii). gapdh i is inhibited 50% by 1.1.10(-3) m koningic acid, while gapdh ii is inhibited 50% at 6.8 x 10(-6) m. cdnas of the two isozymes were cloned from t. koningii and their nucleotide sequences were determined. the sequence of coding region and codon usage in both clon ...	1993	8439569
antifungal metabolites from trichoderma harzianum.	a detailed examination of the metabolites produced in liquid cultures by a strain of trichoderma harzianum, isolated from wheat roots, has resulted in the identification of a further five metabolites. two of these, cyclonerodiol [5] and the octaketide keto diol 6, have previously been isolated from a strain of trichoderma koningii. the structures of the three new octaketide-derived compounds 7, 8, and 10 have been deduced from spectroscopic and chemical studies. all newly isolated compounds show ...	1993	8277317
respiratory symptoms, immunological responses, and aeroallergen concentrations at a sawmill.	after identification of a case of extrinsic allergic alveolitis due to exposure to wood dust at a sawmill, all employees at the sawmill where he worked were studied with an occupational, environmental, and symptom questionnaire, spirometry, skin prick tests, and serum specific igg measurements. ninety five of current and 14 of 17 ex-sawmill workers were studied. as a basis for comparison, a group of 58 workers from a nearby light engineering factory were also studied. few women (6) were employed ...	1994	8130844
extrinsic allergic alveolitis and asthma in a sawmill worker: case report and review of the literature.	a 34 year old sawmill maintenance engineer developed a dry cough that was associated with widespread wheezes and crackles in his lungs. his symptoms worsened, with work related lethargy, fever, and breathlessness, and the loss of a stone in weight. at that time, while still at work, he had a neutrophil leucocytosis and increased concentration of gamma globulins. when seen subsequently some two months after stopping work, his chest radiograph and lung function tests were normal, but the cells rec ...	1994	8130843
molecular cloning and sequence analysis of the cellobiohydrolase i gene from trichoderma koningii g-39.	the cellobiohydrolase i gene, cbh1, has been cloned from an enhanced cellulase-producing strain, trichoderma koningii g-39. sequence comparisons show that t. koningii cbh1 is identical to that of t. reesei with the exception of 6 bp--two causing silent substitutions in the coding region, three differing in one of the introns, and one in 5'-noncoding region. thus, it should encode an identical cbhi to that of t. reesei despite the differences in morphological characters of the two species. analys ...	1994	7764306
studies on metabolites of mycoparasitic fungi. ii. metabolites of trichoderma koningii.	four peptaibols, named trichokonins (tks) v, vi, vii, and viii, were isolated from the culture broth of trichoderma koningii oudemans. primary structures of these peptaibols were elucidated by electrospray ionization mass spectrometry (esi-ms), fab-ms, and collision-induced dissociation (cid) techniques along with nuclear overhauser enhancement spectroscopy (noesy).	1995	7728928
trichokonin vi, a new ca2+ channel agonist in bullfrog cardiac myocytes.	the effects of trichokonin vi (= gliodeliquescin a), a peptaibol isolated from the culture broth of trichoderma koningii oudemans, on l-type ca2+ channel currents in single bullfrog atrial cells were investigated. our results showed that trichokonin vi is a new potent agonist of l-type ca2+ channels in cardiac membranes.	1994	7698190
koninginin c: a biologically active natural product from trichoderma koningii.	koninginin c, a congener of koninginins a and b, was isolated from trichoderma koningii fermented on a shredded wheat medium. the compound inhibited the growth of etiolated wheat coleoptiles by 100% at 10(-3) m. it was a fine, white crystalline substance with a molecular formula of c16h28o4 and a melting point of 70-72 degrees c.	1995	7612999
the action on cellulose and its derivatives of a purified 1,4-beta-glucanase from trichoderma koningii.	the specific properties have been examined of the 1,4-beta-glucanase component of trichoderma koningii that participates in an early and effective stage of random breakdown of native cellulose to short fibres. the enzyme was purified and freed from associated components of the cellulase complex (particularly beta-glucosidase) that interfere with, and complicate interpretation of, the action of such enzymes. purification increased the specific activity 25-fold over culture filtrates; the enzyme h ...	1981	7200359
[detection of transglycosylation reactions under the influence of trichoderma koningii and trichoderma viride cellulase].	the transglycosylation reactions catalyzed by the low molecular weight endo-beta-1,4-glucanase from the cellulase complex of trichoderma koningii as well as unseparated complexes from tr. koningii and tr. viride were investigated. the products obtained from the mixture of cellodextrine (donor, 0.5%) and p-nitrophenyl-beta-d-glucopyranoside (acceptor, 0.4%) with the aid of the above enzymes were analyzed. transglycosylation products were separated by sephadex g-15 gel-filtration. in all the three ...	1981	7197022
[purification and properties of low molecular weight endoglucanase of the cellulase complex from trichoderma koningii].	a homogenous low molecular weight 1,4-beta-glucan glucanohydrolase (endoglucanase) has been isolated from a crude commercial preparation of cellokoningine p10x of t. koningii origin. the molecular weight of the enzyme as determined by polyacrylamide gel electrophoresis is 13 000. the endoglucanase was obtained as a lyophylized preparation free of the cellobiase activity. it was shown that cellobiose or methylcellobioside activate the effect of the low molecular weight endoglucanase (measured by ...	1980	7189672
[isolation and comparative properties of serine proteinases of the microscopic fungi trichoderma lignorum and trichoderma koningii].	using affinity chromatography on bacitracin-sepharose combined with ion-exchange chromatography on aminosilochrome and isoelectrofocusing, individual serine proteases have been isolated for the first time from surface cultures of t. lignorum and t. koningii. the proteinases have pi values at 6.8 and 6.7 and ph optima of 10.5. both proteinases are stable within the ph range of 4-11 and have molecular weight of 21 000. the amino acid composition of t. lignorum enzyme is lys3his4arg9asx23thr17ser23 ...	1981	7032610
cellulolytic enzymes of trichoderma koningii.		1980	6776043
affinity chromatography of proteolytic enzymes on silica-based biospecific sorbents.	new biospecific sorbents for affinity chromatography of proteolytic enzymes were prepared by the attachment of the cyclopeptide antibiotics bacitracin, bacilliquin or gramicidin s to aminosilochrom via a reaction with p-benzoquinone. the content of the cyclopeptide ligands within the sorbents varied from 2 to 46 mumol/g. the sorbents prepared by this reaction were successfully applied in the purification of the carboxylic proteinases produced by fungi, russula decolorans (a basidiomycete) and tr ...	1981	6271864
solubilization of native and derived forms of cellulose by cell-free microbial enzymes.	1. cell-free enzymes from myrothecium verrucaria and trichoderma koningii hydrolyse native undegraded cellulose, as found in cotton fibres, in a random manner to short insoluble fibres and to minor amounts of soluble products. 2. enzyme preparations from m. verrucaria fail to attack the short fibres whereas preparations from t. koningii solubilize them completely to sugars at an optimum ph4.2-4.6. 3. the mode of hydrolysis of cotton cellulose by preparations from t. koningii involves from the ea ...	1966	5968532
cellulolytic enzyme system of trichoderma koningii. separation of components attacking native cotton.	1. cell-free culture filtrates from trichoderma koningii were concentrated by precipitation with ammonium sulphate between the limits of 20% and 80% saturation. 2. removal of a low-molecular-weight carboxymethylcellulase (cm-cellulase) component by chromatography on sephadex g-75 had no effect on the ability of the enzyme complex to solubilize cotton. 3. further chromatography on deae-sephadex separated a component (c(1)) from the c(x) (cm-cellulase) and beta-glucosidase activities. separately t ...	1968	5692804
the formation of short fibres from native cellulose by components of trichoderma koningii cellulase.	cellulolytic enzyme components of culture filtrates of trichoderma koningii were fractionated on ionic and non-ionic forms of sephadex and on cellulose powder (whatman) and examined for their ability to hydrolyse soluble carboxymethyl-cellulose, and to saccharify, solubilize and form short fibres from native undegraded cellulose of the type found in cotton. deae-sephadex provided two cm-cellulase components and a c(1) component; the c(1) component acted weakly and solely on cotton, forming solub ...	1970	5460790