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characterization of foot and mouth disease virus serotype sat-2 in swamp water buffaloes (bubalus bubalis) under the egyptian smallholder production system.spontaneous mutations are a common characteristic of the foot and mouth disease virus (fmdv), leading to wide antigenic variations resulting in the emergence of new topotypes and lineages of fmdv, which contributes to occasional vaccination failures. the objectives of the present study were to genetically characterize fmdv isolated from water buffaloes and study the biochemical and histopathological indicators of infected animals. fifty-four water buffaloes of both sexes and different ages suffe ...202134200281
viral infections shared between water buffaloes and small ruminants in switzerland.importation of exotic animals that may harbor infectious agents poses risks for native species with potentially severe impacts on animal health and animal production. although the asian water buffalo (bubalus bubalis) population in europe is steadily increasing, its susceptibility to viral infections and its role for interspecies transmission is largely unknown. to identify viral infections that are shared between exotic water buffaloes and native small ruminants, we collected blood samples from ...202134166139
evaluation of safety and efficacy of an inactivated marker vaccine against bovine alphaherpesvirus 1 (bohv-1) in water buffalo (bubalus bubalis).recent studies have explored the seropositivity of bovine alphaherpesvirus 1 (bohv-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. however, in europe, the glycoprotein e (ge) deleted marker vaccines against bohv-1 are commercially available only for the cattle industry. this study, for the first time, evaluated the safety and efficacy of a commercial inactivated ge-deleted marker vaccine in water buffalo. f ...202133917160
prevalence of foot and mouth disease virus in apparently healthy buffaloes brought to islamabad slaughterhouse in pakistan.this study was conducted to determine the sero-prevalence of foot and mouth disease (fmd) in buffaloes (bubalus bubalis) slaughtered at islamabad slaughterhouse, pakistan. sera and oro-pharyngeal (op) fluid were collected from 630 healthy buffaloes. these animals originated from various livestock markets from 14 districts of punjab. the age of animals ranged from 1 to 15 years. sera were analyzed using fmd virus (fmdv) nonstructural proteins (nsp) and indirect elisa. the sero-prevalence of fmd w ...201833601788
structural analysis of covid-19 spike protein in recognizing the ace2 receptor of different mammalian species and its susceptibility to viral infection.the pandemic covid-19 was caused by a novel coronavirus-2 (sars-cov-2) that infects humans through the binding of glycosylated sars-cov-2 spike 2 protein to the glycosylated ace2 receptor. the spike 2 protein recognizes the n-terminal helices of the glycosylated metalloprotease domain in the human ace2 receptor. to understand the susceptibility of animals for infection and transmission, we did sequence and structure-based molecular interaction analysis of 16 ace2 receptors from different mammali ...202133552834
whole genome re-sequencing reveals adaptation prior to the divergence of buffalo subspecies.the application of high throughput genotyping or sequencing data helps us to understand the genomic response to natural and artificial selection. in this study, we scanned the genomes of five indigenous buffalo populations belong to three recognized breeds, adapted to different geographical and agro-ecological zones in iran, to unravel the extent of genomic diversity and to localize genomic regions and genes underwent past selection. a total of 46 river buffalo whole genomes, from west and east ...202033179728
assessment of multicolor flow cytometry panels to study leukocyte subset alterations in water buffalo (bubalus bubalis) during bvdv acute infection.the identification of cross-reactive monoclonal antibodies (mabs) that recognize orthologous leukocyte differentiation molecules (ldm) in buffaloes has overcome a major impediment limiting research on the immune response to pathogens and development of vaccines. as reported, two pilot trials were conducted to accomplish two objectives: (1) demonstrate that multiparameter flow cytometry can be conducted equally well in buffalo with mabs directly and indirectly labeled with fluorochromes in resear ...202033178728
determination and ecological risk assessment of two endocrine disruptors from river buffalo, south africa.4-tert-octylphenol (4-top) and triclosan (tcs) are endocrine disruptors which have been detected in environmental matrices such as air, soil and water at ultra-low levels. exposure to endocrine disruptors may account at least in part, for the global increase in the incidence of non-communicable diseases like cancers and diabetes and may also lead to an imbalance in the aquatic ecosystem. river buffalo is an important natural resource in the eastern cape of south africa serving more than half a m ...202033155083
optimization and comparison of three-dimensional culture conditions in different media of coculture and encapsulation system for in vitro follicular development in bubalus bubalis.the establishment of an in vitro culture system for complete oocyte maturation from the early stages of ovarian follicles is still a challenge. the aim of the present study was to assess the effect of different matrix with different culture media on the developmental growth of ovarian follicles in vitro. an ovarian histoarchitectural study was carried out to identify the primordial (0.027-0.039 mm), primary (0.041-0.079 mm), small preantral (0.085-0.131 mm), large preantral (0.132-0.294 mm), sma ...202033147076
fsh stimulation with short withdrawal improves oocyte competence in italian mediterranean buffalo (bubalus bubalis).the aim of this work was to evaluate the efficiency of different fsh doses and fsh coasting times before ovum pick-up (opu) on follicular growth and oocyte competence in buffalo. experiment 1 involved two different fsh treatments: 40 mg fsh given three (fsh3) or six (fsh6) times, 2 days after dominant follicle removal were tested, with opu carried out after 40-44 h of coasting. in experiment 2, opu was carried out after fsh6 protocol followed by 28-32 h (c1), 40-44 h (c2), or 64-68 h (c3) of coa ...202033143113
integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (bubalus bubalis).in mammals, sperm need to mature in the epididymis to gain fertilization competency. however, the molecular mechanism underlying buffalo sperm maturation remains elusive. exploring sperm physiology at the posttranslational modification (ptm) level could help to develop our understanding of these mechanisms. protein phosphorylation and ubiquitination are major ptms in the regulation of many biological processes. in the present study, to our knowledge, we report the first phosphoproteome and ubiqu ...202033140506
synthesis of iron oxide nanoparticles-antiubiquitin antibodies conjugates for depletion of dead/damaged spermatozoa from buffalo (bubalus bubalis) semen.the synthesis of iron oxide nanoparticles (ionps)-antiubiquitin antibodies (abs) complex for depletion of dead/damaged spermatozoa from buffalo semen was done. the ionps synthesized were round in shape with size of 12.09 ± 0.91 nm. at the end of the two-step functionalization, that is, silanization and pegylation of bare ionps and bioconjugation of functionalized iopns, particles with the sizes of 19.15 ± 1.46, 20.72 ± 0.95, and 73.01 ± 7.56 nm, respectively, were obtained. twenty-four semen sam ...202033135803
intra-articular injection techniques of the buffalo (bubalus bubalis) hindlimb digit using anatomical and contrast arthrography-guided landmarks.this study was designed to evaluate and compare the optimal sites for intra-articular (ia) injection into the digits of buffalo by discrimination of the injection criteria. forty-eight cadaveric hind digits of adult buffalos and nine live ones were assigned for three trial investigations. in the first division, eighteen sound cadaveric limbs were used to describe the anatomical features of the hind digit. in the second division, thirty cadaveric limbs (ten for each approach) were injected with a ...202033128523
contrasting population genetics of co-endemic cattle- and buffalo- derived theileria annulata.a study was designed to improve understanding of the genetics of theileria annulata populations in sympatric cattle and asian buffalo (bubalus bubalus). the study was undertaken in the punjab province of pakistan, where the prevalence of tropical theileriosis is high. parasite materials were collected from infected animals in defined regions, where cattle and asian buffalo are kept together. six satellite dna markers and a mitochondrial cytochrome b marker were used to explore the multiplicity o ...202033113478
comparative transcriptome analysis reveals that pck1 is a potential gene affecting imf deposition in buffalo.in china, although buffaloes are abundant, beef is mainly obtained from cattle, and this preference is mainly attributed to the low intramuscular fat (imf) content of buffalo. genetic factors are an important driver that affects imf deposition.202033045988
reducing ruminal ammonia production with improvement in feed utilization efficiency and performance of murrah buffalo (bubalus bubalis) through dietary supplementation of plant-based feed additive blend.the study evaluated the potential of blends of eucalyptus oil and aqueous extract of mulethi (root of glycyrrhiza glabra) to reduce rate of ruminal ammonia production without affecting feed digestion to improve nitrogen utilization efficiency and performance of murrah buffalo (bubalus bubalis). based on preliminary independent studies with graded doses of eucalyptus oil and mulethi root aqueous extract in modulating in vitro rumen fermentation, four blends of feed additive comprising graded dose ...202033015136
innovative use and efficiency test of subcutaneous transponders for electronic identification of water buffaloes.the objective of this study was to evaluate the viability of using transponders for the electronic identification of water buffaloes and compare their efficiency when used in animals of different age groups. electronic transponders with rfid technology (2.1 × 12.2 mm) were implanted subcutaneously (d0) in the scutiform cartilage. the animals consisted of four groups: clf-i (17 calves; 2.1 ± 1.9 months), clf-ii (20 calves; 5.1 ± 3.2 months), hfr (20 heifers; 22 ± 4.7 months) and str (19 steers; 2 ...202033009587
antibiotic resistance and genotyping of meca-positive methicillin-resistant staphylococcus aureus (mrsa) from milk and nasal carriage of dairy water buffaloes (bubalus bubalis) in the philippines.mastitis is considered as an economically important disease of dairy buffaloes in asia. this study examined the mastitis milk and nasal swab samples for the detection and genotyping of methicillin-resistant staphylococcus aureus (mrsa) in water buffaloes.202033005664
parthenogenetic activation of buffalo (bubalus bubalis) oocytes: comparison of different activation reagents and different media on their developmental competence and quantitative expression of developmentally regulated genes.this study was carried out to compare the efficacy of different methods to activate buffalo a + b and c + d quality oocytes parthenogenetically and to study the in vitro developmental competence of oocytes and expression of some important genes at the different developmental stages of parthenotes. the percentage of a + b oocytes (62.16 ± 5.06%, range 53.8-71.3%) was significantly higher (p < 0.001) compared with that of c + d oocytes (37.8 ± 5.00%, range 28.6-46.1%) retrieved from slaughterhouse ...202033004105
granulosa cell-conditioned medium enhances steroidogenic competence of buffalo (bubalus bubalis) theca cells.granulosa cells (gcs) and theca cells (tcs) are the main components of follicles, and the interactions between gcs and tcs play a significant role in steroidogenesis, follicular growth, and atresia. however, the effects of gcs in the form of conditioned medium on steroidogenesis in buffalo tcs remain unclear. in the present study, the impacts of gc-conditioned medium (gccm) on androgen synthesis in buffalo tcs were examined. the results showed that gccm collected at 48 h promoted both the expres ...202033000385
novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo (bubalus bubalis).to understand mechanisms of spermatogenesis, the proteome and the phosphoproteome in prepubertal and pubertal swamp buffalo (bubalus bubalis) testes were analyzed using tandem mass tag (tmt) coupled with liquid chromatography-tandem mass spectrometry (lc-ms/ms). in prepubertal testes, 80 proteins were overexpressed, 148 proteins were underexpressed, and 139 and 142 protein sites had higher and lower phosphorylation, respectively, compared to the levels in pubertal testes. several of these protei ...202032973212
ovarian dynamics of buffalo (bubalus bubalis) synchronized with different hormonal protocols.the study evaluates the effect of three hormonal protocols on ovarian dynamics and progesterone (p4) secretion of buffalo (bubalus bubalis). twenty-nine pluriparous murrah buffaloes were used. the protocols were as follows: ovsynch (n = 10): 100 μg of gonadorelin (day 0), 500 μg of cloprostenol (day 7), and 100 μg of gonadorelin (day 9). cidr+eb (intravaginal device (cidr®) + estradiol benzoate; n = 10): cidr plus 2 mg of eb (day 0), withdrew of cidr, 500 μg of cloprostenol (day 7) and 1 mg of e ...202032949356
spermatozoa produced during winter are superior in terms of phenotypic characteristics and oviduct explants binding ability in the water buffalo (bubalus bubalis).although reduced reproductive efficiency during summer has been well documented in buffaloes, the reason for the same is yet to be understood. the present study was conducted to identify the subtle differences in sperm phenotypic characteristics (motility, membrane integrity, acrosome reaction and lipid peroxidation status), oviduct binding ability and expression of fertility-associated genes (ak 1, atp5d, catsper 1, cytochrome p450 aromatase, spp1 and pebp1) between winter and summer seasons in ...202032945545
evaluation of the immune responses against reduced doses of brucella abortus s19 (calfhood) vaccine in water buffaloes (bubalus bubalis), india.brucella abortus s19 is the most widely used vaccine for the prevention of bovine brucellosis which remains the reference vaccine to which many other vaccine/s are compared. considering the larger vaccination coverage by reduced dose of vaccine, the study aimed to compare reduced graded doses (1/10th, 1/20th and 1/100th) with standard dose of s19 vaccine (40 × 109cfu /dose) to determine the effective immunizing dose in water buffaloes.202032943264
effect of basic fibroblast growth factor (fgf2) on cumulus cell expansion, in vitro embryo production and gene expression in buffalo (bubalus bubalis).the present study was undertaken to evaluate the effect of different concentration of fgf2 viz. 5 ng (t1), 10 ng (t2), and 20 ng/ml (t3) on cumulus cell expansion, oocyte maturation, in vitro embryo production, total cell number (tcn) of the blastocyst, and expression of the fgf2 and fgfr2 transcripts in buffalo oocytes and the embryos. results showed that the effect of fgf2 on the diameter of buffalo coc was significantly higher (p < 0.05) in the t1 group than the other groups at 24h of maturat ...202032921625
comparative gene expression profiling of milk somatic cells of sahiwal cattle and murrah buffaloes.india is the world's largest milk producing country because of massive contribution made by cattle and buffaloes. in the present investigation, comprehensive comparative profiling of transcriptomic landscape of milk somatic cells of sahiwal cattle and murrah buffaloes was carried out. genes with highest transcript abundance in both species were enriched for biological processes such as lactation, immune response, cellular oxidant detoxification and response to hormones. analysis of differential ...202132877747
selection and validation of reference genes for rt-qpcr in adipose and longissimus dorsi muscle tissues of buffalo.real-time quantitative pcr (rt-qpcr) is widely used to measure and evaluate gene expression. the precision and reliability of rt-qpcr are critically dependent on the selection of suitable reference genes (rgs). in this study, an effort was made to identify the optimal rgs for rt-qpcr analysis of adipose and the longissimus dorsi muscle (lm) in buffaloes. rna sequencing data were firstly analyzed to obtain 10 candidate genes (fkbp1a, c25h16orf72, pnrc2, iqgap1, atp5pd, rpl6, ndufb4, tra2a, caprin ...202032862779
identification of genomic regions related to age at first calving and first calving interval in water buffalo using single-step gblup.in brazil, water buffaloes have been used to produce milk for mozzarella cheese production. consequently, the main selection criterion applied for the buffalo genetic improvement is the estimated mozzarella yield as a function of milk, fat and protein production. however, given the importance of reproductive traits in production systems, this study aimed to use techniques for identifying genomic regions that affect the age at first calving (afc) and first calving interval (fci) in buffalo cows a ...202032853485
a review of toxoplasmosis and neosporosis in water buffalo (bubalus bubalis).toxoplasmosis and neosporosis are diseases with worldwide distribution that are associated with reproductive problems in livestock and responsible for economic losses. this review presents an overview of the current knowledge relative to these diseases in water buffalo (bubalus bubalis). in general, buffalo are considered resistant to clinical toxoplasmosis because there are studies only reporting serological evidence of natural infection in these animals. studies have described age, poor hygien ...202032851033
effect of dickkopf-1 and colony stimulating factor-2 on the developmental competence, quality, gene expression and live birth rate of buffalo (bubalus bubalis) embryos produced by hand-made cloning.a functional canonical wnt signaling pathway exists in preimplantation embryos and inhibits embryonic development. recent studies suggest that this pathway is over-expressed in nuclear transferred (nt), compared to ivf embryos. the present study investigated the effects of dickkopf-1 (dkk1), an inhibitor of canonical wnt signaling pathway and colony stimulating factor-2 (csf2), an embryokine, on the developmental competence, quality, gene expression and live birth rate of nt buffalo embryos prod ...202032823021
semen parameters and fertility potency of a cloned water buffalo (bubalus bubalis) bull produced from a semen-derived epithelial cell.semen contains epithelial cells that can be cultured in vitro. for somatic cell nuclear transfer applications, it is essential to know whether clone(s) produced from semen-derived epithelial cells (sedecs) are healthy and reproductively competent. in this study, the semen and fertility profile of a cloned bull (c1) that was produced from a sedec were compared with its donor (d1) and with two cloned bulls (c2, c3) that were produced from commonly used skin-derived fibroblast cells (skdfcs). we ob ...202032822364
sequencing and assembly of the egyptian buffalo genome.water buffalo (bubalus bubalis) is an important source of meat and milk in countries with relatively warm weather. compared to the cattle genome, a little has been done to reveal its genome structure and genomic traits. this is due to the complications stemming from the large genome size, the complexity of the genome, and the high repetitive content. in this paper, we introduce a high-quality draft assembly of the egyptian water buffalo genome. the egyptian breed is used as a dual purpose animal ...202032813723
investigation of the role of aromatic residues in the antimicrobial peptide bucathl4b.antimicrobial peptides (amps) are an attractive alternative to traditional small molecule antibiotics as amps typically target the bacterial cell membrane. a trp-rich peptide sequence derived from water buffalo (bubalus bubalis), bucathl4b was previously identified as a broad-spectrum antimicrobial peptide.202032798369
effect of animal age, postmortem chilling rate, and aging time on meat quality attributes of water buffalo and humped cattle bulls.the study was aimed to investigate the influence of animal age, post-slaughter chilling rate, and aging time on meat quality of m. longissimus dorsi (ld) of water buffalo (bubalus bubalis) and humped cattle (bos taurus indicus) bulls. after slaughtering, one side of carcasses was subjected to rapid chilling (rc) (0 ± 2°c) and other side was hanged in controlled room temperature (25 ± 2°c) for 3 hr, then allowed to the chiller (0 ± 2°c). the meat quality traits were analyzed at 1, 7, and 14 days ...202032789990
seasonal effects on mirna and transcriptomic profile of oocytes and follicular cells in buffalo (bubalus bubalis).season clearly influences oocyte competence in buffalo (bubalus bubalis); however, changes in the oocyte molecular status in relation to season are poorly understood. this study characterizes the microrna (mirna) and transcriptomic profiles of oocytes (oos) and corresponding follicular cells (fcs) from buffalo ovaries collected in the breeding (bs) and non-breeding (nbs) seasons. in the bs, cleavage and blastocyst rates are significantly higher compared to nbs. thirteen mirnas and two mrnas show ...202032782284
remote monitoring system as a tool for calving management in mediterranean buffalo heifers (bubalus bubalis).buffalo breeding is common in southern italy. dystocia compromises dam's and newborn health and welfare. difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. herein, we aimed to evaluate a remote calving alarm system in 15 mediterranean buffalo heifers. an intravaginal probe was placed close to the external cervical os once premonitory signs of delivery were observed. no vaginal discharge nor signs of dis ...202032780888
escherichia coli membrane-derived oxygen-reducing enzyme system (oxyrase) protects bubaline spermatozoa during cryopreservation.the objective of this study was to determine the effectiveness of deoxygenation of semen extender using escherichia coli membrane-derived oxygen scavenger (oxyrase) on post-thaw quality of buffalo (bubalus bubalis) spermatozoa. sixteen semen ejaculates, four each from four bulls, were each divided into five equal fractions, diluted using tris-egg yolk extender supplemented with different concentrations of oxyrase (0, 0.3, 0.6, 0.9, and 1.2 u/ml), designated as treatments t1, t2, t3, t4, and t5, ...202032780495
molecular characterization, tissue expression and polymorphisms of buffalo ppargc1a gene.ppargc1a exerts important functions in activating many nuclear receptors and transcription factors that are related to energy balance. previous studies have shown that ppargc1a gene is associated with lactation traits of dairy cattle. however, the functional role of the buffalo ppargc1a gene is still unknown. in this work, the complete coding sequence (cds) of buffalo ppargc1a was isolated and characterized for swamp and river buffalo. the cds length of ppargc1a for both types of buffalo was the ...202032775610
theca cell-conditioned medium added to in vitro maturation enhances embryo developmental competence of buffalo (bubalus bubalis) oocytes after parthenogenic activation.theca cells (tcs) play a key role in follicular growth and atresia. tcs synthesize androgens that act as substrate for granulosa cells (gcs) aromatization to estrogens needed for oocyte maturation. however, the effects of tcs in the form of conditioned medium on in vitro maturation (ivm) and developmental competence of buffalo oocytes remain unclear. in the present study, we examined the impacts of tc-conditioned medium (tccm) on maturation efficiency and embryo development of buffalo oocytes af ...202032767798
feeding preferences and nutritional niche of wild water buffalo (bubalus arnee) in koshi tappu wildlife reserve, nepal.the nutritional characteristics of food resources play an important role in the foraging behavior of animals and can provide information valuable to their conservation and management. we examined the nutritional ecology of wild water buffalo (bubalus arnee; hereafter "buffalo") in the koshi tappu wildlife reserve of nepal during autumn using a multidimensional nutritional niche framework. we identified 54 plant species as being foraged by buffalo. we found that buffalo consumed graminoids and fo ...202032760500
theca cell-conditioned medium enhances steroidogenesis competence of buffalo (bubalus bubalis) granulosa cells.theca cells (tcs) play a crucial role in follicular development and atresia. tcs synthesize the androgens that act as substrate for granulosa cells (gcs) aromatization to estrogens needed for follicular growth. however, the effects of tcs in the form of conditioned medium on steroidogenesis in buffalo gcs remain unclear. in the present study, the impacts of tc-conditioned medium (tccm) on estrogen synthesis in buffalo gcs were examined. the results showed that tcs secreted principally testostero ...202032748525
diversity analysis at mhc class ii dqa locus in buffalo (bubalus bubalis) indicates extensive duplication and trans-species evolution.variation at mhc class ii-dqa locus in riverine and swamp buffaloes (bubu) has been explored in this study. through sequencing of buffalo dqa, 48 nucleotide variants identified from 17 individuals, reporting 42 novel alleles, including one pseudogene. individual animal displayed two to seven variants, suggesting the presence of more than two bubu-dqa loci, as an evidence of extensive duplication. dn values were found to be higher than ds values at peptide binding sites, separately for riverine a ...202032738270
evolution and domestication of the bovini species.domestication of the bovini species (taurine cattle, zebu, yak, river buffalo and swamp buffalo) since the early holocene (ca. 10 000 bce) has contributed significantly to the development of human civilization. in this study, we review recent literature on the origin and phylogeny, domestication and dispersal of the three major bos species - taurine cattle, zebu and yak - and their genetic interactions. the global dispersion of taurine and zebu cattle was accompanied by population bottlenecks, w ...202032716565
expression pattern of glut 1, 5, 8 and citrate synthase transcripts in buffalo (bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo.in vitro-produced (ivp) embryos are reported to be developmentally lesser competent than in vivo-derived (ivd) embryos and supposed to differ in the expression of genes related with glucose metabolism. so, the present study was conducted to analyse the expression pattern of glut 1, 5, 8 and citrate synthase (cs) in oocytes and embryos produced in vivo or in vitro in buffalo. ivd embryos were obtained from 18 superovulated buffaloes. ivp embryos were obtained from slaughterhouse-derived oocytes s ...202032706414
molecular diagnosis and prevalence of trypanosoma vivax (trypanosomatida: trypanosomatidae) in buffaloes and ectoparasites in the brazilian amazon region.trypanosoma vivax ziemann is a parasite that affects both wild and domestic ungulates and is transmitted mechanically via tabanids and other blood-sucking insects in the americas. a total of 621 blood samples from water buffaloes (bubalus bubalis (linnaeus) (artiodactyla: bovidae), and 184 ectoparasite samples (amblyomma cajennense (fabricius) sensu stricto and rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae), and haematopinus tuberculatus (burmeister) (phthiraptera: haematopin ...202032696974
influence of  uterine torsion in water buffaloes (bubalus bubalis) with insights into the hematological and biochemical prognostic values regarding to manual correction.bovine uterine torsion refers to the rotation of the gravid uterus around its longitudinal axis. it is the dominant cause of dystocia in buffaloes. a total of two hundred and seventeen dystocia's egyptian buffaloes were examined, and another twenty were apparently normal at the full term (healthy pregnant group). from the dystocia's buffaloes, twenty-three percentage (n = 50) was uterine torsion after gynecology inspection at presentation (ut group) and 5 days after the manual correction (utc gr ...202032677006
activation of wnt/β-catenin signaling pathway enhances the derivation of buffalo (bubalus bubalis) embryonic stem cell-like cells.wnt/β-catenin signaling pathway plays an important role in maintaining self-renewal and pluripotency of human and mouse embryonic stem cells (escs). activation of wnt/β-catenin signaling pathway by glycogen synthase kinase-3 (gsk3) inhibitor, the wnt signaling agonist, could maintain the pluripotency of human and mouse escs in the presence of serum. however, the role of signaling pathway in the derivation of buffalo escs remains unclear. in this study, we used gsk3 inhibitors (6-bromoindirubin-3 ...202032673062
a comparative study on rumen ecology of water buffalo and cattle calves under similar feeding regime.in all, 12 male water buffalo (bubalus bubalis) calves and holstein (bos taurus) calves of similar age (10 ± 5 days) were selected to explore the mechanism underlying the differences in growth performance and feed conversion ratio between the two species. the experiment contains 33 days of sucking period and 40 days of post-weaning period. both calves were fed the same amounts of milk in sucking period, and starter and oat grass were supplied ad libitum both before and after the weaning period. ...202032657053
early prediction of corpus luteum functionality using an imaging software.the present study aimed to assess the applicability of luteal blood flow data acquired through the use of color doppler ultrasonography and a post-processing analysis tool (imagej) for predicting pregnancy in buffaloes (bubalus bubalis). the experiment was carried out on 59 multiparous italian mediterranean buffaloes that underwent synchronization of estrus and fixed-time artificial insemination (tai). corpus luteum features (size: cls and blood flow: bfa) were taken from day 5 to 10 after tai a ...202032626727
passive immunity transfer in water buffaloes (bubalus bubalis).this study aimed to evaluate passive immunity transfer in healthy buffalo calves. colostrum samples from heifers (without previous calving) and primiparous and pluriparous dams and blood samples from their offspring were obtained at calving, before colostrum intake, and at 24, 48, and 72 h after calving for determination of serum activities of gammaglutamyltransferase and alkaline phosphatase and serum concentrations of total protein (tp), immunoglobulin a (iga) and igg, and lactoferrin. the res ...202032626726
effect of synergism between carboxylated poly-l-lysine and glycerol on freezability of nili-ravi buffalo (bubalus bubalis) semen.carboxylated poly-l-lysine (cpll), an ampholytic polymer, has remarkable cryoprotective properties. it was hypothesized that cpll will reduce/replace glycerol in extender and improve the freezability of buffalo semen. the objective was to evaluate various combinations of cpll and glycerol in extenders for any synergism toward cryosurvivability of nili-ravi buffalo sperm. semen collected from four nili-ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based exte ...202032609000
identification of sarcocystis spp. in one-humped camels (camelus dromedarius) from riyadh and dammam, saudi arabia, via histological and phylogenetic approaches.sarcocystis(s.) spp. are intracellular protozoan parasites that infect birds and animals, resulting in substantial commercial losses. sarcocystis spp. have an indirect life cycle; canines and felines are known to act as final hosts, and numerous domestic and wild animals act as intermediate hosts. the presence of sarcocysts in camel meat may diminish its commercial quality. there is limited knowledge regarding the taxonomy and diagnosis of sarcocystis spp. that infect camels in saudi arabia. in ...202032605261
caffeic acid improves microscopic sperm parameters and antioxidant status of buffalo (bubalus bubalis) bull semen following freeze-thawing process.this study investigates the effects of cryopreservation and supplementation of buffalo's semen with caffeic acid. it studies the effects of different caffeic acid concentrations on cryopreservation capacity of the buffalo and evaluates their influence on various sperm parameters like motility, viability, progressive motility, sperm plasma membrane integrity, and antioxidant status. twenty-four semen samples were collected with an artificial vaginal from three adult water buffalos. the semen samp ...202032590017
first record of natural infection with anaplasma marginale in sucking lice infesting the water buffalo (bubalus bubalis) in mexico.the water buffalo (bubalus bubalis) is an asian species of bovine which was introduced in mexico in 1992, as an alternative for milk and meat production. however, little is known about its associated ectoparasites, in particular about their lice. as a part of a project to identify the diversity and prevalence of anaplasma in cattle and water buffaloes in the state of veracruz, mexico, we visited a ranch in the downtown area in which 42 buffaloes were visually inspected for the presence of ectopa ...202032588174
high trypanosoma vivax infection rates in water buffalo and cattle in the brazilian lower amazon.highly sensitive and accurate molecular diagnostic methods have not yet been employed for livestock trypanosomosis in the brazilian lower amazon although the first reports of trypanosoma vivax and trypanosoma evansi in brazil were in water buffalo (bubalus bubalis) in this region. the present study assessed trypanosomosis in buffalo and cattle raised in communal and seasonally flooding pastures in the state of pará using the fluorescent fragment length barcoding (fflb) method. t. evansi was not ...202032565339
additional characterization of the adult worm mammomonogamus laryngeus (railliet, 1899) and the tissue lesions caused by the infection in buffaloes.mammomonogamus spp. are parasites with curious characteristics, such as the "y" shape that results from male and female maintaining the permanent copulation position and the controversial presence or absence of spicules. these nematodes are hematophagous and cause damage to the upper respiratory tracts of cattle, sheep, goats, deer, wild yaks, and orangutans. human infection is rare and most cases until now have been in the caribbean islands or in brazil, and mainly in farmworkers but recently t ...202032544763
extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes.extracellular vesicles (evs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. the aim of the present report was to provide the first isolation, quantification and protein characterization of evs from buffalo (bubalus bubalis) sera infected with theileria spp.202032528536
identification of pathogenic leptospira species in the urogenital tract of water buffaloes (bubalus bubalis) from the amazon river delta region, brazil.in the current context of deforestation and fire in the amazon, buffaloes could be a cost-effective and sustainable alternative for cattle production in the region, as they can convert low-quality foods and be raised in floodplain areas. however, little is known about the reproductive diseases that affect these animals; thus, the purpose of this study was to perform the molecular characterization of leptospira spp. in the urogenital tract of water buffaloes (bubalus bubalis) raised in the amazon ...202032478114
rumen fluke, fischoederius elongatus (trematoda: gastrothylacidae): preliminary investigation of suitable conditions for egg hatching.various temperatures may have different effects on the distribution of paramphistomes that cause amphistomosis in cattle, including fischoederius elongatus. therefore, this study aimed to investigate the effects of different temperature treatments on f. elongatus hatching, with specific identification using morphological, histological, and phylogenetic analysis. all specimens were collected from two buffalo (bubalus bubalis) rumens in a slaughterhouse in pathum thani province, thailand. f. elong ...202032470871
ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (bubalus bubalis) testis.protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. although several ubiquitinated proteins have been found in buffalo (bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. in the present study, we first identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive ...202032469046
evaluation and mining the applicable methods of roughage digestibility determination for buffalo (bubalus bubalis).buffalo is an amazing ruminant with high tolerance to low-quality roughage. due to the special breeding environment, there are few reports on the digestibility of roughage in buffalo because it is difficult to quantify ingestion and egestion. to find more applicable method to determine the digestibility of low-quality roughage in buffalo, this study was conducted to compare total feces collection (tfc) method with the following three indirect techniques: cr2o3 (chromic oxide), aia (acid-insolubl ...202032458351
effects of feeding and maturation system on qualitative characteristics of buffalo meat (bubalus bubalis).we aimed to evaluate the effects of post dry ageing (pda) period on meat colour and rheological characteristics in 16 buffalo bulls fed two different diets: with (frs) or without (ctl) rye grass. animals were randomly divided into two feeding groups and slaughtered at 540 ± 4.7 and 533 ± 7.0 kg of live body weight, respectively, for the ctl and frs group. after five days post-mortem ageing (t0), semitendinosus muscle (st) and longissimus muscle (ld) underwent a prolonged maturation process in a ...202032455847
linkage disequilibrium-based inference of genome homology and chromosomal rearrangements between species.the aim of this study was to analyze the genomic homology between cattle (bos taurus) and buffaloes (bubalus bubalis) and to propose a rearrangement of the buffalo genome through linkage disequilibrium analyses of buffalo snp markers referenced in the cattle genome assembly and also compare it to the buffalo genome assembly. a panel of bovine snps (single nucleotide polymorphisms) was used for hierarchical, non-hierarchical and admixture cluster analyses. thus, the linkage disequilibrium informa ...202032434754
generation of transgenic cloned buffalo embryos harboring the egfp gene in the y chromosome using crispr/cas9-mediated targeted integration.sex control technology is of great significance in the production of domestic animals, especially for rapidly breeding water buffalo (bubalus bubalis), which served as a research model in the present study. we have confirmed that a fluorescence protein integrated into the y chromosome is fit for sexing pre-implantation embryos in the mouse. firstly, we optimized the efficiency of targeted integration of exogenous gene encoding enhanced green fluorescent protein (egfp) and mcherry in neuro-2a cel ...202032426378
cryptosporidium and giardia in feral water buffalo (bubalus bubalis) in the south east arnhem land indigenous protected area, australia.global investigations have implicated water buffalo (bubalus bubalis) as a potential source of zoonotic cryptosporidium and giardia parasites which may pose a threat to human health. in australia, buffalo are a feral pest that have colonised the floodplains, wetlands and woodlands of indigenous owned and managed arnhem land, in tropical monsoonal northern australia. indigenous people from the remote community ngukurr have raised concerns about the potential threat to their health from shared use ...202032424553
sequencing of lipoprotein lipase gene in the mediterranean river buffalo identified novel variants affecting gene expression.lipoprotein lipase (lpl) is a key enzyme for lipid metabolism, playing a fundamental role in the composition of fat in adipose tissue and milk. the lpl gene has been seldom investigated in dairy ruminants and barely studied in river buffalo (bubalus bubalis). the aim of this work was to explore the genetic diversity of lpl and its promoter and to identify functional mutations, using a combined approach based on sequencing, dual-color electrophoretic mobility shift assay, and quantitative pcr. th ...202032418698
the addition of resveratrol in tris citric acid extender ameliorates post-thaw quality parameters, antioxidant enzymes levels, and fertilizing capability of buffalo (bubalus bubalis) bull spermatozoa.resveratrol is a natural grape-derived polyphenol with potent antioxidant properties that protect spermatozoa against lipid peroxidation (lpo) by eradicating free radicals. the objectives of this study were to 1) appraise the effects of resveratrol in extender on post-thaw quality parameters, antioxidant enzymes, adenosine triphosphate (atp), dna fragmentation, lpo and 2) fertilizing capability of buffalo bull spermatozoa. semen was collected from four fertility proven bulls with artificial vagi ...202032388038
genomic identification, evolution, and expression analysis of collagen genes family in water buffalo during lactation.collagens, as extracellular matrix proteins, support cells for structural integrity and contribute to support mammary basic structure and development. this study aims to perform the genomic identification, evolution, and expression analyses of the collagen gene family in water buffalo (bubalus bubalis) during lactation. a total of 128 buffalo collagen protein sequences were deduced from the 45 collagen genes identified in silico from buffalo genome, which classified into six groups based on thei ...202032384775
analysis of meiotic segregation by triple-color fish on both total and motile sperm fractions in a t(1p;18) river buffalo bull.chromosomal aberrations are relatively frequent pathologies in both humans and animals. among them, translocations present a specific meiotic segregation pattern able to give a higher percentage of unbalanced gametes that can induce fertility problems. in this study, the meiotic segregation patterns of 1p, 1q and 18 bubalus bubalis chromosomes were analyzed in both total sperm fraction and motile sperm fraction of a t(1p;18) carrier and a control bulls by triple-color fish analysis with a pool o ...202032365118
the domesticated buffalo - an emerging model for experimental and therapeutic use of extraembryonic tissues.large animals play important roles as model animals for biomedical sciences and translational research. the water buffalo (bubalus bubalis) is an economically important, multipurpose livestock species. important assisted reproduction techniques, such as in vitro fertilization, cryo-conservation of sperm and embryos, embryo transfer, somatic cell nuclear transfer, genetic engineering, and genome editing have been successfully applied to buffaloes. recently, detailed whole genome data and transcri ...202032320839
multivariate analysis of body morphometric traits in conjunction with performance of reproduction and milk traits in crossbred progeny of murrah × jafarabadi buffalo (bubalus bubalis) in north-eastern brazil.we studied the relationship between body morphometric traits, and their underlying association with milk production (mp), lactation length (ll), first calving interval (fci) and subsequent calving interval (ci) of crossbred progeny of murrah × jafarabadi buffalo aiming to assist in selection programs. we carried out principal component analysis (pca) of the body morphometric traits, which include breast width (bw), thigh width (thw), hip width (hw), rump width (rw), rump length (rl), body depth ...202032315344
abatement of enteric methane production from lactating murrah buffaloes (bubalus bubalis) with improving production performance and immune status through dietary supplementation of composite feed additive.ruminant livestock production processes are the major sources of methane production in agriculture sector triggering global environmental pollution. above 90% of world buffalo population present in asian countries, india ranks first and contributes significantly to the environmental pollution by enteric methane emissions. in this study, we examined the effect of dietary composite feed additive supplementation on ruminal methane production, nutrient utilization, milk production and immune status ...202032314293
high prevalence of subclinical paratuberculosis in buffaloes (bubalus bubalis) in maranhão, brazil.paratuberculosis is an infectious, chronic, and incurable disease that affects ruminants, causing enteritis and chronic granulomatous lymphadenitis, characterized by malabsorption syndrome, its agent is the mycobacterium avium subsp. paratuberculosis (map). thus, the objective of this work was to identify and characterize map in buffalo herds slaughtered in baixada maranhense region. samples of intestines, mesenteric lymph nodes, and ileocecal valves were collected from 115 buffaloes slaughtered ...202032291741
efficient transovarial transmission of babesia spp. in rhipicephalus microplus ticks fed on water buffalo (bubalus bubalis).water buffaloes can be infected by tick-borne pathogens (tbps) in endemic areas where cattle and buffalo coexist. among tbps affecting buffaloes is the apicomplexan hemoparasites babesia bovis and b. bigemina, transmitted by rhipicephalus microplus ticks. however, little empirical evidence exists on whether buffalo can support tbps' infection and transmission. a cohort study was designed to measure the infestation levels of r. microplus in buffaloes as well as the ability of buffalo-fed ticks to ...202032290468
mitochondrial sequence-based evolutionary analysis of riverine-swamp hybrid buffaloes of india indicates novel maternal differentiation and domestication patterns.in this study, mitochondrial d-loop sequence data on riverine, swamp and hybrid buffaloes from india have been generated and compared with other reported indian riverine, chinese and bangladeshi swamp buffalo populations. sequence analysis revealed the presence of 132 haplotypes, with a haplotype diversity of 0.9611 ± 0.0045 and a nucleotide diversity of 0.04801 ± 0.00126. for the first time, the existence of riverine-swamp hybrids among the indian chilika buffalo population has been recorded, h ...202032281135
correction to: genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in iranian river buffalo, bubalus bubalis.following publication of the original article [1], the authors flagged that the article had published with the author 'ali jalil sarghale' erroneously omitted from the author list.202032268878
bovine papillomavirus type 2 infection associated with papillomatosis of the amniotic membrane in water buffaloes (bubalus bubalis).multiple papillomatous nodules were observed scattered over the amniotic membrane in six water buffaloes that had recently aborted. grossly, some of the nodules had multiple villous projections while others appeared as single prominent conical or cylindrical horns. histology revealed folded hyperplastic and hyperkeratotic epithelium supported by a narrow fibro-vascular stalk. using pcr, sequences of the bovine deltapapillomavirus type 2 (bpv-2) e5 gene were amplified from the amniotic papillomas ...202032260380
recombinant vaccine against botulism in buffaloes: evaluation of the humoral immune response over 12 months.botulism is a neuroparalytic intoxication, usually fatal, caused by the botulinum toxins (bonts). vaccination is the best-known strategy to prevent this disease in ruminants. serotypes c and d and their variants cd and dc are the main types responsible for botulism in bovine and buffaloes in brazil and cattle in japan and europe. brazil has a herd of approximately 1.39 million buffaloes and is the largest producer in the western world. this study aimed to assess the humoral immune response of bu ...202032247696
seroprevalence and clinical outcomes of neospora caninum, toxoplasma gondii and besnoitia besnoiti infections in water buffaloes (bubalus bubalis).one hundred twenty-four water buffaloes (bubalus bubalis) originating from 9 farms located in southern italy were tested to investigate simultaneously, for the first time, the seroprevalence of the protozoa neospora caninum, toxoplasma gondii and besnoitia besnoiti by elisa tests and to evaluate the clinical findings potentially associated to the presence of these aborting parasitic pathogens. twenty-five of 124 buffaloes (20.2%) were positive for n. caninum, while 17/124 (13.7%) for t. gondii. ...202032235734
identification, molecular characteristics, and tissue differential expression of dgat2 full-cds cdna sequence in binglangjiang buffalo (bubalus bubalis).it has been found that diacylglycerol acyltransferase-2 (dgat2) plays a crucial role in the synthesis of triglycerides (tgs) in some mammals, but its role in buffalo lactation is unclear. in the present study, the dgat2 full-cds cdna sequence of binglangjiang buffalo was isolated, and the physicochemical characteristics and structure of its encoding protein were characterized. furthermore, the differential expressions of this gene in 10 tissues of lactating and non-lactating buffalo were analyze ...202032232120
milk somatic cell count and its relationship with milk yield and quality traits in italian water buffaloes.in southern italy, buffalo (bubalus bubalis) milk is mostly intended for the manufacture of mozzarella di bufala campana protected denomination of origin (pdo) cheese. despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare. milk somatic cell count (scc) is used worldwide as an indicator of udder health in individual milk an ...202032229124
modulation of immunity and inflammatory gene expression in buffalo (bubalus bubalis) with some digestive disorders.so far, there has been scarce information about the status of immunoglobulins (ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. the purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. for this reason, 50 native breed water buffaloes were studied. forty of these buffaloes showed various symptoms of digestive disorders and were allocate ...201732214977
evaluation of the middle ear in water buffalos (bubalus bubalis) by gross anatomy and cone beam computed tomography.the purpose of this study was to provide a description of gross middle ear morphology in water buffalos, augmented with additional data on the osseous structures of middle ear derived from cone-beam computed tomography (cbct).202032207849
poly i:c stimulation in-vitro as a marker for an antiviral response in different cell types generated from buffalo (bubalus bubalis).the innate immune system is activated upon virus invasion of a host cell by recognizing viral component, such as dsrna through specific receptors, resulting in the production of type- i ifns, which confer an antiviral state within the invaded as well as surrounding cells. in the present study, fibroblast, monocyte and macrophage cells derived from water buffalo (bubalus bubalis) were exposed to a synthetic dsrna analogue, poly i:c to mimic viral invasion in each cell type. recognition of poly i: ...202032200171
characterization of drug resistance-associated tevat1 gene of trypanosoma evansi from philippine water buffaloes (bubalus bubalis)this study detected and characterized the tevat1 gene of trypanosoma evansi isolates from philippine water buffaloes (bubalus bubalis). a total of 68 blood samples from philippine water buffaloes were subjected to dna extraction and pcr assay was performed using rotat 1.2 gene to detect t. evansi. those samples positive for t. evansi subsequently underwent another pcr assay to detect the presence of tevat1 gene. trypanosoma evansi was detected in 26.47% (18/68) blood samples in which distributed ...201932191985
in-depth proteome analysis of more than 12,500 proteins in buffalo mammary epithelial cell line identifies protein signatures for active proliferation and lactation.the mature mammary gland is made up of a network of ducts that terminates in alveoli. the innermost layer of alveoli is surrounded by the differentiated mammary epithelial cells (mecs), which are responsible for milk synthesis and secretion during lactation. however, the mecs are in a state of active proliferation during pregnancy, when they give rise to network like structures in the mammary gland. buffalo (bubalus bubalis) constitute a major source of milk for human consumption, and the mecs a ...202032179766
first description of giardia duodenalis in buffalo calves (bubalus bubalis) in southwest region of são paulo state, brazil.we performed molecular characterization of giardia duodenalis in buffalo calves from the southwest region of são paulo state, brazil. a total of 183 fecal samples of murrah breed buffaloes up to six months of age were collected. we examined these samples by the polymerase chain reaction (pcr) targeting the small-subunit ribosomal rna gene and positive samples were characterized using additional pcr assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phospha ...201932095632
comparative modeling and mutual docking of structurally uncharacterized heat shock protein 70 and heat shock factor-1 proteins in water buffalo.in this study, a wide range of in silico investigation of bubalus bubalis (bb) heat shock protein 70 (hsp70) and heat shock factor-1 (hsf1) has been performed, ranging from sequence evaluation among species to homology modeling along with their docking studies to decipher the interacting residues of both molecules.201932095057
genomic analyses reveal distinct genetic architectures and selective pressures in buffaloes.the domestic buffalo (bubalus bubalis) is an essential farm animal in tropical and subtropical regions, whose genomic diversity is yet to be fully discovered.202032083286
glutathione-s-transferase: an important diagnostic antigen of liver amphistome gigantocotyle explanatum, infecting the indian water buffalo.the foodborne trematodiases pose a significant health problem to the animals as well as the human population living in close proximities with the livestock and are still considered as the neglected tropical diseases by the world health organisation. the digenetic trematode, gigantocotyle explanatum infecting the liver of indian water buffalo, bubalus bubalis, has been identified as one of the most common helminth parasite responsible for the disease, amphistomosis, in livestock. despite huge aba ...202032081660
phylogenetic and evolutionary analysis of hobi-like pestivirus: insights into origin and dispersal.the hobi-like pestivirus (hobipev), currently classified as pestivirus h species, is a pathogen associated with a broad spectrum of clinical manifestations in ruminants, particularly in cattle. since hobipev complete genome sequencing data is scarce, in the present study we described five nearly complete new brazilian hobipev genomes and further perform a more complete genetic and evolutionary characterization with all additional genome sequences available in the genbank database. entropy and se ...202032073749
toxocara vitulorum infection in a yak (bos mutus grunniens) calf from tyrol (austria).the domestic yak bos mutus grunniens is an important livestock animal in parts of asia, especially of the himalayan region, where people rely on it for meat, wool, milk and labour. in its countries of origin, the yak is commonly infected with the ascarid toxocara vitulorum. this parasite mainly infects cattle (bos taurus) and domestic buffalo (bubalus bubalus) and is most commonly found in sub-tropical regions, but has been occasionally reported in more temperate climates, including several cent ...202032057397
genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in iranian river buffalo, bubalus bubalis.consecutive homozygous fragments of a genome inherited by offspring from a common ancestor are known as runs of homozygosity (roh). roh can be used to calculate genomic inbreeding and to identify genomic regions that are potentially under historical selection pressure. the dataset of our study consisted of 254 azeri (az) and 115 khuzestani (khz) river buffalo genotyped for ~ 65,000 snps for the following two purposes: 1) to estimate and compare inbreeding calculated using roh (froh), excess of h ...202032041535
detection of polymorphism within leptin gene in egyptian river buffalo and predict its effects on different molecular levels.leptin (lep) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. the present investigation studied the polymorphism in lep gene which is related to fertility in 81 female egyptian river buffalo.202032037476
comparison of ruminal digestibility of origanum onites l. leaves in dairy buffalo and cows.this experiment evaluated the ruminal digestibility of turkish oregano leaves in dairy buffalo and cows. ruminally cannulated, multiparous brown swiss cows (n = 3) and water buffalo (bubalus bubalis; n = 3) were used in the experiment. the ad libitum basal diet was balanced to nrc requirements for a dry, nonpregnant multiparous dairy cow (680 kg) and consuming 12.8 kg of dm/day. air-dried, ground, weighed oregano leaves were inserted in the rumen of all animals before the morning feeding within ...202032030523
investigating genetic variability in hsp70 gene-5'-fragment and its association with thermotolerance in murrah buffalo (bubalus bubalis) under sub-tropical climate of india.the present study was undertaken to investigate genetic variability in a fragment comprising 5'utr along with partial coding sequence of hsp70 gene and its association with thermotolerance traits in murrah buffalo at icar-research complex for eastern region, patna (india). the allelic variants were identified from genomic dna samples using sscp technique. the pcr products were sequenced and analyzed. data on different thermotolerance traits recorded in three seasons were analyzed by least square ...202032020511
embryo transfer in buffalo (bubalus bubalis).the use of assisted reproductive technologies, such as superovulation and in vivo embryo production and in vitro embryo production (ivep), has increased rapidly in recent years and is now applied worldwide for genetic improvement in beef and dairy buffaloes. although in vivo embryo production has been shown to be feasible in buffalo, low efficiency and limited commercial application has been documented. these results could be associated with low antral follicle populations, high levels of follic ...202031996292
heat stress modulates differential response in skin fibroblast cells of native cattle (bos indicus) and riverine buffaloes (bubalus bubalis).heat stress in hot climates is a major cause that negatively affects dairy animals, leading to substantial economic loss. the present study was aimed to analyze the effect of heat stress on cellular and molecular levels in dermal fibroblast of cattle and buffaloes. primary fibroblast culture was established using ear pinna tissue samples of cattle (bos indicus) and riverine buffaloes (bubalus bubalis). the cells were exposed to thermal stress at 42°c for 1 h and subsequently allowed to recover a ...202031994693
asian water buffalo: domestication, history and genetics.the domestic asian water buffalo (bubalus bubalis) is found on all five continents, with a global population of some 202 million. the livelihoods of more people depend on this species than on any other domestic animal. the two distinct types (river and swamp) descended from different wild asian water buffalo (bubalus arnee) populations that diverged some 900 kyr bp and then evolved in separate geographical regions. after domestication in the western region of the indian subcontinent (ca. 6300 ye ...202031967365
effects of treatment with a microrna mimic or inhibitor on the developmental competence, quality, epigenetic status and gene expression of buffalo (bubalus bubalis) somatic cell nuclear transfer embryos.expression levels of 13 micrornas (mirnas) were compared between buffalo blastocysts produced by somatic cell nuclear transfer through hand-made cloning and ivf to improve cloning efficiency. expression of mir-22, mir-145, mir-374a and mir-30c was higher, whereas that of mir-29b, mir-101, mir-302b, mir-34a, mir-21 and mir-25 was lower, in nuclear transferred (nt) than ivf embryos; the expression of mir-200b, mir-26a and mir-128 was similar between the two groups. based on these, mir-145, which i ...202031959280
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