Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| microbiomic analysis of intra-abdominal infections by using denaturing high-performance liquid chromatography: a prospective observational study. | intra-abdominal infections represent a subgroup of septic syndromes with high death rates and the need for prompt and appropriate antimicrobial therapy. conventional culture-based microbial identification has notable shortcomings in the diagnostics of polymicrobial infections. modern culture-independent molecular methods may represent a new diagnostic approach. the current study aimed to compare the results obtained from the denaturing high-performance liquid chromatography wave(®) system as a c ... | 2017 | 28375806 |
| rapid analysis of bile acids in different biological matrices using lc-esi-ms/ms for the investigation of bile acid transformation by mammalian gut bacteria. | bile acids are important signaling molecules that regulate cholesterol, glucose, and energy homoeostasis and have thus been implicated in the development of metabolic disorders. their bioavailability is strongly modulated by the gut microbiota, which contributes to generation of complex individual-specific bile acid profiles. hence, it is important to have accurate methods at hand for precise measurement of these important metabolites. here, a rapid and sensitive liquid chromatography-tandem mas ... | 2017 | 27822648 |
| microbial community of healthy thai vegetarians and non-vegetarians, their core gut microbiota, and pathogen risk. | pyrosequencing analysis of intestinal microflora from healthy thai vegetarians and non-vegetarians exhibited 893 otus covering 189 species. the strong species indicators of vegetarians and non-vegetarians were prevotella copri and bacteroides vulgatus as well as bacteria close to escherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belongi ... | 2016 | 27381339 |
| structural and biochemical insights into 7β-hydroxysteroid dehydrogenase stereoselectivity. | hydroxysteroid dehydrogenases are of great interest as biocatalysts for transformations involving steroid substrates. they feature a high degree of stereo- and regio-selectivity, acting on a defined atom with a specific configuration of the steroid nucleus. the crystal structure of 7β-hydroxysteroid dehydrogenase from collinsella aerofaciens reveals a loop gating active-site accessibility, the bases of the specificity for nadp(+) , and the general architecture of the steroid binding site. compar ... | 2016 | 27006087 |
| oral versus intravenous iron replacement therapy distinctly alters the gut microbiota and metabolome in patients with ibd. | iron deficiency is a common complication in patients with ibd and oral iron therapy is suggested to exacerbate ibd symptoms. we performed an open-labelled clinical trial to compare the effects of per oral (po) versus intravenous (iv) iron replacement therapy (irt). | 2017 | 26848182 |
| antibiotic resistance genes and human bacterial pathogens: co-occurrence, removal, and enrichment in municipal sewage sludge digesters. | understanding which/how antibiotic resistance genes (args) contribute to increased acquisition of resistance by pathogens in aquatic environments are challenges of profound significance. we explored the co-occurrence and removal versus enrichment of args and human bacterial pathogens (hbps) in municipal sewage sludge digesters. we combined metagenomic detection of a wide spectrum of 323 args and 83 hbps with a correlation-based statistical approach and charted a network of their co-occurrence re ... | 2016 | 26773390 |
| dietary isomers of sialyllactose increase ganglioside sialic acid concentrations in the corpus callosum and cerebellum and modulate the colonic microbiota of formula-fed piglets. | sialyllactose is a key human milk oligosaccharide and consists of sialic acid (sa) bound to a lactose molecule. breastfed infants have increased accumulation of ganglioside-bound sa compared with formula-fed infants. | 2016 | 26701794 |
| membrane filter method to study the effects of lactobacillus acidophilus and bifidobacterium longum on fecal microbiota. | a large number of commensal bacteria inhabit the intestinal tract, and interbacterial communication among gut microbiota is thought to occur. in order to analyze symbiotic relationships between probiotic strains and the gut microbiota, a ring with a membrane filter fitted to the bottom was used for in vitro investigations. test strains comprising probiotic nitto strains (lactobacillus acidophilus nt and bifidobacterium longum nt) and type strains (l. acidophilus jcm1132(t) and b. longum jcm1217( ... | 2015 | 26486646 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents - special references to bacteria isolated between april 2011 and march 2012]. | bacteria isolated from surgical infections during the period from april 2011 to march 2012 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 785 strains including 31 strains of candida spp. were isolated from 204 (78.8%) of 259 patients with surgical infections. five hundred and twenty three strains were isolated from primary infections, and 231 strains were isolated from surgical site infection. from primary infections, anaerobic gram-ne ... | 2014 | 25796741 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2010 and march 2011]. | bacteria isolated from surgical infections during the period from april 2010 to march 2011 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 631 strains including 25 strains of candida spp. were isolated from 170 (81.7%) of 208 patients with surgical infections. four hundred and twenty two strains were isolated from primary infections, and 184 strains were isolated from surgical site infection. from primary infections, anaerobic gram-nega ... | 2014 | 25549405 |
| dynamics and diversity of the 'atopobium cluster' in the human faecal microbiota, and phenotypic characterization of 'atopobium cluster' isolates. | this study monitored the dynamics and diversity of the human faecal 'atopobium cluster' over a 3-month period using a polyphasic approach. fresh faecal samples were collected fortnightly from 13 healthy donors (six males and seven females) aged between 26 and 61 years. fish was used to enumerate total (eub338mix) and 'atopobium cluster' (ato291) bacteria, with counts ranging between 1.12×10(11) and 9.95×10(11), and 1.03×10(9) and 1.16×10(11) cells (g dry weight faeces)(-1), respectively. the 'at ... | 2015 | 25533445 |
| the influence of whole grain products and red meat on intestinal microbiota composition in normal weight adults: a randomized crossover intervention trial. | intestinal microbiota is related to obesity and serum lipid levels, both risk factors for chronic diseases constituting a challenge for public health. we investigated how a diet rich in whole grain (wg) products and red meat (rm) influences microbiota. during a 10-week crossover intervention study, 20 healthy adults consumed two isocaloric diets, one rich in wg products and one high in rm. repeatedly data on microbiota were assessed by 16s rrna based denaturing gradient gel electrophoresis (dgge ... | 2014 | 25299601 |
| metabolic niche of a prominent sulfate-reducing human gut bacterium. | sulfate-reducing bacteria (srb) colonize the guts of ∼50% of humans. we used genome-wide transposon mutagenesis and insertion-site sequencing, rna-seq, plus mass spectrometry to characterize genetic and environmental factors that impact the niche of desulfovibrio piger, the most common srb in a surveyed cohort of healthy us adults. gnotobiotic mice were colonized with an assemblage of sequenced human gut bacterial species with or without d. piger and fed diets with different levels and types of ... | 2013 | 23898195 |
| multi-enzymatic one-pot reduction of dehydrocholic acid to 12-keto-ursodeoxycholic acid with whole-cell biocatalysts. | ursodeoxycholic acid (udca) is a bile acid of industrial interest as it is used as an agent for the treatment of primary sclerosing cholangitis and the medicamentous, non-surgical dissolution of gallstones. currently, it is prepared industrially from cholic acid following a seven-step chemical procedure with an overall yield of <30%. in this study, we investigated the key enzymatic steps in the chemo-enzymatic preparation of udca-the two-step reduction of dehydrocholic acid (dhca) to 12-keto-urs ... | 2013 | 22806613 |
| novel whole-cell biocatalysts with recombinant hydroxysteroid dehydrogenases for the asymmetric reduction of dehydrocholic acid. | ursodeoxycholic acid is an important pharmaceutical so far chemically synthesized from cholic acid. various biocatalytic alternatives have already been discussed with hydroxysteroid dehydrogenases (hsdh) playing a crucial role. several whole-cell biocatalysts based on a 7α-hsdh-knockout strain of escherichia coli overexpressing a recently identified 7β-hsdh from collinsella aerofaciens and a nad(p)-bispecific formate dehydrogenase mutant from mycobacterium vaccae for internal cofactor regenerati ... | 2012 | 22581067 |
| functional metagenomics reveals novel salt tolerance loci from the human gut microbiome. | metagenomics is a powerful tool that allows for the culture-independent analysis of complex microbial communities. one of the most complex and dense microbial ecosystems known is that of the human distal colon, with cell densities reaching up to 10(12) per gram of faeces. with the majority of species as yet uncultured, there are an enormous number of novel genes awaiting discovery. in the current study, we conducted a functional screen of a metagenomic library of the human gut microbiota for pot ... | 2012 | 22534607 |
| dysbiosis of the faecal microbiota in patients with crohn's disease and their unaffected relatives. | a general dysbiosis of the intestinal microbiota has been established in patients with crohn's disease (cd), but a systematic characterisation of this dysbiosis is lacking. therefore the composition of the predominant faecal microbiota of patients with cd was studied in comparison with the predominant composition in unaffected controls. whether dysbiosis is present in relatives of patients cd was also examined. | 2011 | 21209126 |
| identification, cloning, heterologous expression, and characterization of a nadph-dependent 7β-hydroxysteroid dehydrogenase from collinsella aerofaciens. | a gene encoding an nadph-dependent 7β-hydroxysteroid dehydrogenase (7β-hsdh) from collinsella aerofaciens dsm 3979 (atcc 25986, formerly eubacterium aerofaciens) was identified and cloned in this study. sequence comparison of the translated amino acid sequence suggests that the enzyme belongs to the short-chain dehydrogenase superfamily. this enzyme was expressed in escherichia coli with a yield of 330 mg (5,828 u) per liter of culture. the enzyme catalyzes both the oxidation of ursodeoxycholic ... | 2010 | 21181147 |
| clostridium perfringens bacteriophages φcp39o and φcp26f: genomic organization and proteomic analysis of the virions. | poultry intestinal material, sewage and poultry processing drainage water were screened for virulent clostridium perfringens bacteriophages. viruses isolated from broiler chicken offal washes (o) and poultry feces (f), designated φcp39o and φcp26f, respectively, produced clear plaques on host strains. both bacteriophages had isometric heads of 57 nm in diameter with 100-nm non-contractile tails characteristic of members of the family siphoviridae in the order caudovirales. the double-strand dna ... | 2010 | 20963614 |
| association of symptoms with gastrointestinal microbiota in irritable bowel syndrome. | to investigate the correlations between self-reported symptoms of irritable bowel syndrome (ibs) and the gastrointestinal (gi) microbiota composition. | 2010 | 20857523 |
| dominant and diet-responsive groups of bacteria within the human colonic microbiota. | the populations of dominant species within the human colonic microbiota can potentially be modified by dietary intake with consequences for health. here we examined the influence of precisely controlled diets in 14 overweight men. volunteers were provided successively with a control diet, diets high in resistant starch (rs) or non-starch polysaccharides (nsps) and a reduced carbohydrate weight loss (wl) diet, over 10 weeks. analysis of 16s rrna sequences in stool samples of six volunteers detect ... | 2011 | 20686513 |
| in vitro inhibition activity of nisin a, nisin z, pediocin pa-1 and antibiotics against common intestinal bacteria. | to evaluate the sensitivity of 21 common intestinal bacteria to six antibiotics and three broad-spectrum bacteriocins (nisins z and a and pediocin pa-1). | 2007 | 17718835 |
| anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor. | the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ... | 2003 | 16887710 |
| preparation of methyl ursodeoxycholate in an interface bioreactor: use of a mixed solvent system to increase the solubilities of substrate and product. | a mixed solvent system was used as the reaction solvent of an interface bioreactor for the first time. the solvent, dodecane/2-ethylhexyl acetate (4:6), exhibited excellent efficiency in the preparation of methyl ursodeoxycholate with eubacterium aerofaciens jcm 7790. | 2001 | 16233065 |
| preparation of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate in an anaerobic interface bioreactor. | an interface bioreactor, which is a device for the microbial transformation of water-insoluble substrates, was applied to an anaerobic bioconversion for the first time. methyl 7-ketolithocholate [me-7klca] was reduced with the human intestinal bacterium eubacterium aerofaciens jcm 7790 in a convenient anaerobic interface bioreactor using a nutrient agar plate placed in a gaspak pouch. the resulting methyl ursodeoxycholate [me-udca] is a precursor of ursodeoxycholic acid, which is used as a chole ... | 2001 | 16232975 |
| synthesis of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate with eubacterium aerofaciens jcm 7790 grown on two kinds of carbon and hydride sources, glucose and mannitol. | eubacterium aerofaciens jcm 7790, which produces 7beta-hydroxysteroid dehydrogenase [7beta-hsdh], efficiently catalyzed the reduction of methyl 7-ketolithocholate [me-7klca] to afford methyl ursodeoxycholate [me-udca] in an anaerobic interface bioreactor. for the high accumulation of me-udca, the best supplement of carbon and hydride source to the abcm medium (glucose content, 0.3 wt.%) was screened because glucose strongly repressed 7beta-hsdh production. the supplementation of cellobiose, mann ... | 2001 | 16232971 |
| metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by pseudomonas aeruginosa and other human endogenous bacteria. | pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-af), 4-aminobiphenyl (4-ab), and benzidine (bz). the cell-free extracts of pseudomonas aeruginosa, escherichia coli, enterobacter aerogenes, proteus mirabilis, proteus vulgaris, staphylococcus epidermidis, staphylococcus saprophyticus, klebsiella pneumoniae, and intestinal anaerobes, ... | 2006 | 16203120 |
| design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples. | an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ... | 2002 | 12167534 |
| mononuclear cell response to enterobacteria and gram-positive cell walls of normal intestinal microbiota in early rheumatoid arthritis and other inflammatory arthritides. | to study whether enterobacteria and gram-positive bacterial cell walls (bcw) derivedfrom normal intestinal microbiota are involved in the etiopathogenesis of early rheumatoid arthritis (ra). | 2002 | 12051398 |
| adhesive property of bifidobacterium lactis lkm512 and predominant bacteria of intestinal microflora to human intestinal mucin. | the adhesive property to the intestinal mucin of bifidobacterium lactis lkm512, b. longum, b. breve, b. bifidum, b. adolescentis, b. infantis, bacteroides vulgatus, bacteroides distasonis, eubacterium aerofaciens, clostridium perfringens, escherichia coli, and lactobacillus acidophilus were examined. adhesive rate of lkm512 to the mucin was significantly (p < 0.05, 0.01, or 0.001) stronger than the other strains from 2 to 100 time. though the adhesive property of many strains was almost same to ... | 2002 | 11821930 |
| enzyme degradation and proinflammatory activity in arthritogenic and nonarthritogenic eubacterium aerofaciens cell walls. | two almost-identical strains of eubacterium aerofaciens isolated from the normal human gut flora were used. the cell wall (cw) of one strain with a peptidoglycan (pg) type a4alpha induces chronic arthritis in the rat after a single intraperitoneal injection, whereas cw of the other with pg type a4beta induces only a transient acute arthritis. the cw of the arthritogenic e. aerofaciens was a twofold-more-potent stimulator of the proinflammatory cytokines tumor necrosis factor alpha (tnf-alpha) an ... | 2001 | 11705898 |
| characterisation of eubacterium cell wall: peptidoglycan structure determines arthritogenicity. | to elucidate factors involved in the arthritogenicity of bacterial cell walls. | 2001 | 11171690 |
| emendation of genus collinsella and proposal of collinsella stercoris sp. nov. and collinsella intestinalis sp. nov. | collinsella aerofaciens-like strains isolated from human faeces were characterized by biochemical tests, cell wall murein analysis and 16s rdna analysis. the results indicated that these strains are phylogenetically a member of the family coriobacteriaceae and close to the genus collinsella. their phenotypic characters resembled those of collinsella aerofaciens. determination of dna-dna relatedness showed that these strains could be divided into two groups (groups 1 and 2). collinsella aerofacie ... | 2000 | 11034485 |
| what determines arthritogenicity of bacterial cell wall? a study on eubacterium cell wall-induced arthritis. | to study what determines the arthritogenicity of the bacterial cell wall (cw) using eubacterium cw-induced arthritis in the rat. | 2000 | 10788535 |
| rapid identification and quantification of collinsella aerofaciens using pcr. | the number and incidence of collinsella aerofaciens in the human intestine are the highest among gram-positive non-spore-forming bacilli. identification of this species is very difficult and requires considerable time. a pcr-based identification system using c. aerofaciens-specific primers is described. using this pcr method, we identified 181 c. aerofaciens-like species isolated from human feces. these 181 strains were identified using the traditional method in past studies. results of both met ... | 2000 | 10650200 |
| phylogenetic evidence for the transfer of eubacterium lentum to the genus eggerthella as eggerthella lenta gen. nov., comb. nov. | eubacterium lentum has unique phenotypic characters within the genus eubacterium. the 16s rrna sequence of eubacterium lentum was determined and its phylogenetic position was defined. this micro-organism is a member of the genus eubacterium but it is not closely related to eubacterium limosum, the type species of the genus eubacterium, and is nearer to collinsella aerofaciens and coriobacterium glomerans. a pcr-based identification system using species-specific primers designed on the basis of d ... | 1999 | 10555354 |
| human cytokine responses induced by gram-positive cell walls of normal intestinal microbiota. | the normal microbiota plays an important role in the health of the host, but little is known of how the human immune system recognizes and responds to gram-positive indigenous bacteria. we have investigated cytokine responses of peripheral blood mononuclear cells (pbmc) to gram-positive cell walls (cw) derived from four common intestinal indigenous bacteria, eubacterium aerofaciens (eu.a. ), eubacterium limosum (eu.l.), lactobacillus casei (l.c.), and lactobacillus fermentum (l.f.). our results ... | 1999 | 10540188 |
| tissue distribution and persistence of arthritogenic and non-arthritogenic eubacterium cell walls. | to study the tissue distribution and persistence of arthritogenic and non-arthritogenic eubacterium cell walls (cws), using arthritogenic eubacterium aerofaciens and non-arthritogenic eubacterium limosum. | 1999 | 10410260 |
| phylogenetic and phenotypic evidence for the transfer of eubacterium aerofaciens to the genus collinsella as collinsella aerofaciens gen. nov., comb. nov. | three strains of eubacterium aerofacien, jcm 10188t, jcm 7790 and jcm 7791, and 178 freshly isolated strains of the eubacterium aerofaciens group from human faeces were characterized by biochemical tests, cell wall peptidoglycan type and 16s rrna analysis. the eubacterium aerofaciens group was divided into four groups by fermentation patterns of sucrose and cellobiose, and were further divided into 16 sub-groups by fermentation patterns of aesculin, salicin and amygdalin. all of the strains of t ... | 1999 | 10319476 |
| subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation. | this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ... | 1998 | 9573815 |
| associations of endodontic symptoms and signs with particular combinations of specific bacteria. | significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ... | 1996 | 9206427 |
| bacteriologic findings in tonsillitis and pericoronitis. | bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ... | 1996 | 8816129 |
| [studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis]. | the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ... | 1993 | 8315808 |
| origin of d-alanine present in urine of mutant mice lacking d-amino-acid oxidase activity. | urine of mutant ddy/dao- mice lacking d-amino-acid oxidase contained 13 times more d-alanine than that of normal ddy/dao+ mice. because d-alanine is a component of bacterial cell walls, the possibility that the urinary d-alanine came from intestinal bacteria was examined. in ddy/dao- mice that were made germ free at birth and reared in a germ-free environment, the quantity of urinary d-alanine was found to be at a low level comparable to that of the normal mice. when these germ-free mice were ma ... | 1993 | 7901999 |
| intestinal floras of populations that have a high risk of colon cancer. | the fecal floras of polyp patients, japanese-hawaiians, north american caucasians, rural native japanese, and rural native africans were compared. the polyp patients and japanese-hawaiians were considered to be groups at high risk of colon cancer, and the rural native japanese and rural native africans were considered to be groups at low risk. the north american caucasians were found to have a flora composition intermediate between these two groups. fifteen bacterial taxa from the human fecal fl ... | 1995 | 7574628 |
| the presence of peptidoglycan-polysaccharide complexes in the bowel wall and the cellular responses to these complexes in crohn's disease. | interestingly, using a monoclonal antibody, peptidoglycan-polysaccharide complexes (ppc) were detected intracellularly in the mucosa and submucosa of the bowel wall of crohn's disease (cd) patients. ppc are the main constituents of the gram-positive bacterial cell wall. these ppc were however detected in the normal bowel wall also. therefore, in this study the hypothesis that an enhanced immune responsiveness to bacterial antigens plays a pivotal role in the induction or the chronicity of cd was ... | 1994 | 7515336 |
| characterization of the cecal bacteria of normal pigs. | one hundred ninety-two isolates from cecal contents of three normal weaned pigs were obtained by means of anaerobic roll tube methods and were characterized. seventy-eight percent of the isolates were gram-negative. the most numerous species isolated from each of the pigs was bacteroides ruminicola. this species accounted for 35% of the isolates that were characterized, and selenomonas ruminantium accounted for 21% of the isolates. other gram-negative bacteria isolated from all three pigs were b ... | 1981 | 7235711 |
| characterization of nadp-dependent 7 beta-hydroxysteroid dehydrogenases from peptostreptococcus productus and eubacterium aerofaciens. | peptostreptococcus productus strain b-52 (a human fecal isolate) and eubacterium aerofaciens atcc 25986 were found to contain nadp-dependent 7 beta-hydroxysteriod dehydrogenase activity. the enzyme was synthesized constitutively by both organisms, and the enzyme yields were suppressed by the addition of 0.5 mm 7 beta-hydroxy bile acid to the growth medium. purification of the enzyme by chromatography resulted in preparations with 3.5 (p. productus b-52, on sephadex g-200) and 1.8 (e. aerofaciens ... | 1982 | 6954878 |
| metabolism of mercapturic acid-pathway metabolites of 2-chloro-n-isopropylacetanilide (propachlor) by gastrointestinal bacteria. | 1. mercapturic acid pathway metabolites of propachlor, labelled with 14c, were incubated with pig caecal contents, small and large intestinal contents, and pure cultures of gastrointestinal bacteria. 2. the glutathione, cysteine, n-acetylcysteine, and the s-oxide of the n-acetylcysteine conjugates of propachlor (2-chloro-n-isopropylacetanilide) were each metabolized by mixed pig caecal micro-organisms to 2-mercapto-n-isopropylacetanilide. 3. the extent of formation of 2-mercapto-n-isopropylaceta ... | 1983 | 6880239 |
| formation of ursodeoxycholic acid from chenodeoxycholic acid by a 7 beta-hydroxysteroid dehydrogenase-elaborating eubacterium aerofaciens strain cocultured with 7 alpha-hydroxysteroid dehydrogenase-elaborating organisms. | a gram-positive, anaerobic, chain-forming, rod-shaped anaerobe (isolate g20-7) was isolated from normal human feces. this organism was identified by cellular morphology as well as fermentative and biochemical data as eubacterium aerofaciens. when isolate g20-7 was grown in the presence of bacteroides fragilis or escherichia coli (or another 7 alpha-hydroxysteroid dehydrogenase producer) and chenodeoxycholic acid, ursodeoxycholic acid produced. time course curves revealed that 3 alpha-hydroxy-7-k ... | 1982 | 6758698 |
| effect of bile acid oxazoline derivatives on microorganisms participating in 7 alpha-hydroxyl epimerization of primary bile acids. | we tested bile acid oxazoline derivatives of chenodeoxycholic (cdc-ox), 7-ketolithocholic (7-klc-ox), ursodeoxycholic (udc-ox), and deoxycholic (dc-ox) as inhibitors of the 7-epimerization of the primary bile acids cholic acid (ca) and cdc in cultures of four species of bacteria and the human fecal flora. the organisms tested elaborate a 7 alpha- and/or 7 beta-hydroxysteroid dehydrogenase (hsdh); they were escherichia coli (7 alpha-hsdh), bacteroides fragilis (7 alpha-hsdh), clostridium absonum ... | 1983 | 6366102 |
| predominant gram-positive bacteria in human feces: numbers, variety, and persistence. | the predominant gram-positive bacteria in 47 fecal specimens from 10 healthy men were studied by microscopic and cultural counts, by the characterization and tentative identification of isolates, and by the use of fluorescein isothiocyanate (fitc)-conjugated globulins prepared using some of the isolates. gram-positive bacteria averaged 10(10.5+/-0.4(sd)/g (wet weight) of feces with significant variation from host to host. characterization of 865 isolates, all strict anaerobes and carbohydrate fe ... | 1974 | 4595760 |
| subacute bacterial endocarditis caused by eubacterium aerofaciens: report of a case. | 1973 | 4571278 | |
| comparison of the fecal microflora in rural japanese and urban canadians. | the fecal microflora of nine rural healthy japanese and eight urban healthy canadians was examined. the two populations ate typical japanese and western diets, respectively. the numbers of eubacteria (p less than 0.01), bifidobacteria (p less than 0.05), bacilli (p less than 0.01), lactobacilli and veillonellae and the frequency of occurrence of bifidobacteria were higher in the japanese than in the canadians. higher numbers of bacteroides and lecithinase-negative clostridia were found in the ca ... | 1986 | 3747865 |
| cell wall fragments from major residents of the human intestinal flora induce chronic arthritis in rats. | to investigate the involvement of human intestinal flora in joint inflammation, cell wall fragments of 9 anaerobic gram positive bacteria of the human fecal flora were prepared and tested for arthropathic properties in the rat. a single intraperitoneal injection of cell wall fragments from eubacterium aerofaciens or bifidobacterium species induced persistent chronic arthritis, in contrast to those from eubacterium rectale, clostridium species and lactobacillus leichmanii. the results show that c ... | 1989 | 2585403 |
| effect of rice fiber on human fecal microflora. | the effects of brown rice, containing fourfold as much dietary fiber as polished rice, on the human fecal microflora were determined. significantly increased numbers of bifidobacterium adolescentis and enterococcus faecalis were observed during the brown rice intake, whereas the total counts and the numbers of bacteroides, eubacterium aerofaciens, and escherichia coli during the intake were lower than those before and after the intake. lower numbers of clostridia and low incidences of clostridiu ... | 1989 | 2547140 |
| experimental immune mediated arthritis in rhesus monkeys. a model for human rheumatoid arthritis? | the induction of experimental arthritis in rhesus monkeys was studied by intradermal immunization of bovine type ii collagen and antigens derived from mycobacterium tuberculosis, streptococcus pyogenes, and eubacterium aerofaciens. the tested bacterial antigens proved to be not arthrogenic. bovine type ii collagen induced clinical arthritis in 50% of the rhesus monkeys. type ii collagen induced arthritis in rhesus monkeys proved to be a potential model to study clinical, serological, histologica ... | 1990 | 2353150 |
| intestinal flora of patients with rheumatoid arthritis: induction of chronic arthritis in rats by cell wall fragments from isolated eubacterium aerofaciens strains. | the composition of the obligate anaerobic intestinal flora of patients with rheumatoid arthritis (ra) differed from that of healthy subjects (hs). total numbers of aerobes as well as anaerobic coccoid rods were found elevated when compared with hs. eubacterium species were found in all stool samples of both groups; bifidobacterium species were present in seven (ra) and eight (hs) out of 10 subjects. from the flora of two ra patients and two hs eubacterium species were isolated and identified. ce ... | 1990 | 2257450 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| histology of joint inflammation induced in rats by cell wall fragments of the anaerobic intestinal bacterium eubacterium aerofaciens. | to study the arthropathic properties of human intestinal bacteria, cell wall fragments (cwf) of the anaerobic bowel bacterium eubacterium aerofaciens were injected intraperitoneally (i.p.) in arthritis-susceptible lewis rats. rat paw joints were subsequently studied for histopathological changes. a persisting synovitis accompanied by marginal erosions of cartilage and bone and a marked periosteal apposition of new bone tissue were the main features of the polyarthritis induced. these results are ... | 1991 | 1784889 |
| arthritis by autoreactive t cell lines obtained from rats after injection of intestinal bacterial cell wall fragments. | t cell lines (b13, b19) were isolated from the lymph nodes of lewis rats 12 days after an arthritogenic injection of cell wall fragments of eubacterium aerofaciens (ecw), a major resident of the human intestinal flora. these cell wall fragments consist of peptidoglycan polysaccharide complexes (ppc). the cell lines that bear the helper phenotype were arthritogenic in knee or ankle joints upon intravenous injection into irradiated lewis recipients. b13 was, however, not arthritogenic in irradiate ... | 1992 | 1733514 |
| influence of decontamination on induction of arthritis in lewis rats by cell wall fragments of eubacterium aerofaciens. arthropathic properties of indigenous anaerobic bacteria. | although the cause (or causes) of rheumatoid arthritis is unknown, many workers have suggested that microorganisms play a part. the intestinal flora in particular has been related to the development of joint inflammation. it has been shown previously that cell wall fragments of several anaerobic gram positive intestinal bacteria of human origin are arthritogenic after a single intraperitoneal injection in lewis rats. the part played by indigenous microflora in this model has now been studied by ... | 1992 | 1586251 |
| immunohistology of joint inflammation induced in rats by cell wall fragments of eubacterium aerofaciens. | after a single intraperitoneal injection of cell wall fragments of eubacterium aerofaciens, a main resident from the human intestinal flora, an acute arthritis develops within 2 days which is followed by a chronic arthritis that lasts at least 90 days. in an earlier report the histological appearance of the joint inflammation during this period has been described. in this study we investigated in more detail the cell types that are involved in the development of arthritis by using cell-type-spec ... | 1992 | 1519039 |
| the endodontic microflora revisited. | the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ... | 1992 | 1479923 |
| fecal bacteria in south african rural blacks and other population groups. | quantitative studies were performed on fecal flora of three population groups consuming different diets. twenty rural black south africans and 22 japanese, representing groups at low risk for carcinoma of the colon, were compared with 41 north americans from a high-risk population. specimens taken immediately after defecation were mixed and processed under anaerobic conditions. after the initial incubation, roll tubes were shipped to the united states for final identification. bacterioides and b ... | 1979 | 447500 |
| urease-producing species of intestinal anaerobes and their activities. | urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ... | 1979 | 36839 |