Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
seasonal distribution and faunistic of ticks in the alashtar county (lorestan province), iran.ticks are non-permanent obligate parasites that have considerable medical-veterinary and zoonosis importance. in this regard a study designed to investigate the distribution and fauna of ticks in the alashtar county in iran from april and march 2014.201729187953
groel typing and phylogeny of anaplasma species in ticks from domestic and wild vertebrates.anaplasma species are globally distributed tick-borne bacteria causing a range of clinical conditions in domestic animals, wildlife, and human. nevertheless, data on presence and distribution of anaplasma strains in ticks are still lacking, especially in the mediterranean region. this study reports the molecular identification, genetic characterization and phylogeny of anaplasma strains of both veterinary and zoonotic importance in ticks collected from domestic and wild hosts sampled in a typica ...201829089249
tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern portugal.ticks are vectors of many human and animal pathogens. the aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern portugal. a total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). overall, the analysis of dna extracts prepared from ticks collected from hosts of 11 different species in the distric ...201728958791
molecular detection of crimean-congo hemorrhagic fever virus in ticks, greece, 2012-2014.crimean-congo hemorrhagic fever virus (cchfv) is transmitted to humans mainly through the bite of infected ticks. in greece, only one clinical case has been observed, in 2008, but the seroprevalence in humans is relatively high (4.2%). to have a first insight into the circulation of cchfv in greece, 2000 ticks collected from livestock during 2012-2014 were tested. cchfv was detected in 36 of the 1290 (2.8%) tick pools (1-5 ticks per pool). two genetic lineages were identified: europe 1 and europ ...201728920177
the effect of excluding ungulates on the abundance of ixodid ticks on wild rabbit (oryctolagus cuniculus).tick abundance depends heavily on host density, so reducing access to the host should reduce tick populations in a determined area. in this study we compared the parasitic index (pi = average number of ticks per rabbit) of two wild rabbit populations separated 16 years ago by a fence keeping ungulates from moving freely. two areas were selected (closed and open) wherein 50 wild rabbits per area were sampled for ticks. the pi in the open area (pi = 989.62) was significantly higher than in the clo ...201728840404
crimean-congo hemorrhagic fever virus in ticks collected from livestock in albania.albania is a balkan country endemic for crimean-congo hemorrhagic fever (cchf). it was shown previously that cchf virus (cchfv) sequences from albanian patients cluster into europe 1 clade. aim of the present study was to test for cchfv ticks collected in several regions of albania, and to determine the genetic lineage(s) of the cchfv strains in relation with their geographic distribution. a total of 726 ticks (366 hyalomma marginatum, 349 rhipicephalus bursa and 11 rhipicephalus sanguineus) col ...201728827176
babesia, theileria, and hepatozoon species in ticks infesting animal hosts in romania.babesia spp., theileria spp., and hepatozoon spp. are tick-transmitted apicomplexan parasites that cause several important diseases in animals. to increase current knowledge about the diversity of tick-transmitted pathogens in romania, we investigated the occurrence of babesia spp., theileria spp., and hepatozoon spp. in a wide range of tick species infesting animal hosts. we collected 852 ticks from 10 different animal species from 20 counties in romania. the assessment was based on detection o ...201728676905
diversity and distribution of ticks from domestic ruminants in lebanon.ticks (acari: ixodidae) are ectoparasites infesting livestock in every geographic area in the world and they are vectors of several viral, bacterial, and protozoan pathogens to animals and humans worldwide. a deep knowledge of the geographical distribution of these arthropods would have a key role in the control of tick-borne diseases. few data are available about tick presence in domestic ruminants in lebanon. the study aimed at providing an analysis of tick presence and distribution in lebanon ...201728675252
partial characterization of tick-borne encephalitis virus isolates from ticks of southern ukraine.tick-borne encephalitis (tbe) is the most common tick-borne viral infection in eurasia; thousands of human cases are annually reported from several european countries. several tick species are vectors of the tick-borne encephalitis virus (tbev), while tbe appears to be spreading from the eurasian continent westward to europe. fifteen study sites were chosen from five territories of southern ukraine, including odessa, mykolaiv, kherson oblast, the autonomous republic of crimea, and sevastopol. ti ...201728654319
molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france.corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ...201728645313
crimean-congo hemorrhagic fever virus in ticks in turkey: a broad range tick surveillance study.in turkey, crimean-congo hemorrhagic fever (cchf) outbreaks started in northern regions in 2002. human cases still continue to increase and the disease spreads in many other provinces of turkey. the ecological role of the vector tick species occurring in turkey is not certain exactly. therefore, we planned a broad range tick study in three different ecological and geographical areas extending from the west black sea regions down to the central anatolia. the aim of this study was to determine and ...201728433738
tick-host conflict: immunoglobulin e antibodies to tick proteins in patients with anaphylaxis to tick bite.tick-borne infectious diseases and allergies are a growing problem worldwide. tick bite allergy has been associated with the direct effect of immunoglobulin e (ige) response to tick salivary antigens, or secondary to the induction of allergy to red meat consumption through ige antibodies against the carbohydrate α-gal (gal α 1-3gal β 1-(3)4glcnac-r). however, despite the growing burden of this pathology, the proteins associated with anaphylaxis to tick bite have not been characterized. to addres ...201728423486
molecular characterization of babesia and theileria species in ticks collected in the outskirt of monte romano, lazio region, central italy.in 2012-2013, an investigation was carried out in the viterbo province, lazio region, on ticks and tick-borne apicomplexan protozoa of the babesia and theileria genera. this followed the reporting of high density of ticks by soldiers operating in a military shooting range, and the signaling by owners and local veterinary authorities of several cases of babesiosis among cattle.201728361802
molecular (ticks) and serological (humans) study of crimean-congo hemorrhagic fever virus in the iberian peninsula, 2013-2015.crimean-congo hemorrhagic fever (cchf) is a viral disease, mainly transmitted through tick bite, of great importance in public health. in spain, crimean-congo hemorrhagic fever virus (cchfv) was detected for the first time in 2010 in hyalomma lusitanicum ticks collected from deer in cáceres. the aim of this study was to investigate the presence of cchfv in ticks from cáceres, and from other spanish areas, and to evaluate the presence of antibodies against the virus in individuals exposed to tick ...201728291670
genetic characterization of rhipicephalus sanguineus (sensu lato) ticks from dogs in portugal.the taxonomic status of the brown dog tick rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as r. sanguineus (sensu lato) until its taxonomic status is resolved. recent genetic studies revealed the existence of more than one lineage of r. sanguineus (s.l.) in temperate countries. in this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas locate ...201728285602
molecular evidence of tick-borne hemoprotozoan-parasites (theileria ovis and babesia ovis) and bacteria in ticks and blood from small ruminants in northern algeria.using qpcr, standard pcr and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from souk ahras, algeria. borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. anaplasma ovis was screened in (38/73, 52%) rhipicephalus bursa and (5/22, 22.7%) r. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. coxiella burnetii tested positive in r. bursa (4/73, 5.5%) a ...201728131376
molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey.blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ...201728082649
establishment of an artificial tick feeding system to study theileria lestoquardi infection.the establishment of good experimental models for theileria sp. infection is important for theileriosis research. routinely, infection of ticks is accomplished by feeding on parasite-infected animals (sheep, cows and horses), which raises practical and ethical problems, driving the search for alternative methods of tick infection. artificial tick feeding systems are based mainly on rearing ticks on host-derived or hand-made artificial membranes. we developed a modified feeding assay for infectin ...201628036364
pcr screening of tick-borne agents in sensitive conservation areas, southeast portugal.the southeast region of portugal, particularly the guadiana valley, is currently the reintroduction territory of lynx pardinus (iberian lynx), one of the most endangered felids in the world that is only found in the iberian peninsula. over the last century, populations have declined, placing l. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. among the aspects taken into account in the establishment of new populations is the sanitary status of the ...201727894847
ticks (acari: ixodidae) infesting cattle and some other domestic and wild hosts on the french mediterranean island of corsica.corsica is a mountainous french island in the north-western mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. despite a favourable context for ticks and tick-borne diseases (tbds), the tick fauna of corsica has not systematically been investigated.201627842608
bacterial pathogens and endosymbionts in ticks.ticks collected from goats in northern greece were tested for the presence of tick-borne bacteria. among adult ticks, 37 (57.8%) were rhipicephalus bursa, 11 (17.2%) dermacentor marginatus, 10 (15.6%) ixodes ricinus, 3 (4.7%) rhipicephalus sanguineus sensu lato and 2 (3.1%) haemaphysalis parva; one (1.6%) rhipicephalus spp. tick was nymph. rickettsia monacensis, rickettsia massilae, anaplasma phagocytophilum and anaplasma platys were detected in i. ricinus and rh. bursa ticks. a variety of coxie ...201727686386
natural anaplasmataceae infection in rhipicephalus bursa ticks collected from sheep in the french basque country.rhipicephalus bursa is one of 79 species of the genus rhipicephalus in the family of ixodidae. in this study, we investigated anaplasmataceae bacteria associated with r. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as r. bursa, all females were partially engorged. we found that 50% of the ticks were positive in the initial anaplasmataceae qpcr screening. bacterial species ...201727666778
prevalence of ixodid ticks on cattle and sheep northeast of iran.a survey was carried out to investigate the prevalence of hard tick species (acari: ixodidae) on cattle and sheep north of iran. the aim of study was to determine the prevalence of hard ticks on cattle and sheep in the mountainous areas of golestan province and their geographical distribution. a total of 26 ticks were collected from 22 infested cattle and 26 ticks were collected from 12 infested sheep during activating seasons of ticks in 2013-2014. the species collected from cattle and sheep we ...201627605782
molecular and parasitological survey of ovine piroplasmosis, including the first report of theileria annulata (apicomplexa: theileridae) in sheep and goats from turkey.blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in adana, gaziantep, and adiyaman provinces in southern turkey. fully engorged female ticks were selected and maintained in an incubator until they oviposited. the tick carcasses and their egg masses were examined. piroplasms compatible with babesia spp. and theileria spp. were observed in both symptomatic and asymptomatic small ruminants. genomic dna ...201627559124
involvement of three esterase genes from panonychus citri (mcgregor) in fenpropathrin resistance.the citrus red mite, panonychus citri (mcgregor), is a major citrus pest with a worldwide distribution and an extensive record of pesticide resistance. however, the underlying molecular mechanism associated with fenpropathrin resistance in this species have not yet been reported. in this study, synergist triphenyl phosphate (tpp) dramatically increased the toxicity of fenpropathrin, suggesting involvement of carboxylesterases (cares) in the metabolic detoxification of this insecticide. the subse ...201627548163
molecular evidence of bartonella species in ixodid ticks and domestic animals in palestine.ticks play an important role in disease transmission as vectors for human and animal pathogens, including the gram-negative pathogen bartonella. here, we evaluated the presence of bartonella in ixodid ticks and domestic animals from palestine. we tested 633 partly engorged ticks and 139 blood samples from domestic animals (dogs, sheep and camels) for bartonella using its-pcr. bartonella dna was detected in 3.9% of the tested ticks. none of the ticks collected from sheep and goats were positive f ...201627540374
temporal and spatial distribution and species diversity of hard ticks (acari: ixodidae) in the eastern region of caspian sea.ticks are important parasites because of their voracious blood-feeding activity, and being as vectors for various agents of diseases in both human and livestock. this study was conducted in a monthly schedule from october 2014 to december 2015, at 45 study sites in three regions bordering the caspian sea, according to the topography including hillside, plain and coastal areas in collecting ticks from the body of sheep. alfa and beta biodiversity indices were calculated and compared between the t ...201627519473
the glutathione-s-transferase, cytochrome p450 and carboxyl/cholinesterase gene superfamilies in predatory mite metaseiulus occidentalis.pesticide-resistant populations of the predatory mite metaseiulus (= typhlodromus or galendromus) occidentalis (arthropoda: chelicerata: acari: phytoseiidae) have been used in the biological control of pest mites such as phytophagous tetranychus urticae. however, the pesticide resistance mechanisms in m. occidentalis remain largely unknown. in other arthropods, members of the glutathione-s-transferase (gst), cytochrome p450 (cyp) and carboxyl/cholinesterase (cce) gene superfamilies are involved ...201627467523
sarcoptes scabiei infestation does not alter the stability of ectoparasite communities.the host represents a heterogeneous ecosystem where multiple parasite species co-occur and interact with each other for space and resources. although these interactions may rule the features of an infracommunity and may shape the infracommunity response to external perturbations, the resilience of ectoparasite communities to new infestations remains poorly explored.201627370780
rickettsia felis, an emerging flea-borne rickettsiosis.rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ...201627340613
crimean-congo hemorrhagic fever in the one-humped camel (camelus dromedarius) in east and northeast of iran.this comprehensive study was conducted on multi-purpose one-humped camel (camelus dromedarius) sera and ticks to assess the epidemiological aspects of the crimean-congo hemorrhagic fever virus (cchfv) in northeast iran.201627308275
first detection of candidatus rickettsia barbariae in the flea vermipsylla alakurt from north-western china.vermipsylla is a genus of the family vermipsyllidae within the order siphonaptera of fleas. vermipsylla alakurt is mainly distributed in alpine pastoral areas of kazakhstan, mongolia, china and nepal, and infests sheep, yaks and horses, causing irritation, poor condition, anaemia and even death. however, to date, no rickettsial agents have been reported in v. alakurt.201627267467
microscopic and molecular detection of theileria (babesia) equi infection in equids of kurdistan province, iran.equine piroplasmosis (ep) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of ep is due to the persistent carrier state. carrier animals to babesia (theileria) equi (laveran 1901) and b. caballi (nuttall, 1910) infestation could be identified by extremely sensitive pcr-based method. the purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from kurdistan province, iran ...027095973
quantitative analysis of babesia ovis infection in sheep and ticks.a quantitative pcr, based on the gene encoding babesia ovis surface protein d (bospd) was developed and applied to investigate the presence of babesia ovis (b. ovis) in its principal vector, the tick rhipicephalus bursa (r. bursa), and in the ovine host. quantification of b. ovis in experimentally-infected lambs showed a sharp increase in parasitemia 10-11 days in blood-inoculated and adult tick-infested lambs, and 24 days in a larvae-infested lamb. a gradual decrease of parasitemia was observed ...201627084469
detection of theileria luwenshuni in sheep from great britain.theileria spp. are tick-borne protozoan parasites of the phylum apicomplexa, order piroplasmida that infect a wide range of wild and domestic animals. in great britain, theileria spp. have been reported from livestock associated with transmission by the tick haemaphysalis punctata. however, these reports have not been associated with disease. this study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north kent.201627075661
tick-borne infections in human and animal population worldwide.the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...201527047089
molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine.ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ...201626969489
sero-epidemiological survey of crimean-congo hemorrhagic fever virus in tunisia.crimean-congo hemorrhagic fever (cchf) is a tick-borne disease associated with a high case fatality rate and transmitted mainly by hyalomma marginatum. the geographical distribution of h. marginatum covers most of the western mediterranean basin. we aimed to investigate whether cchf virus (cchfv) is circulating in tunisia. samples from unexplained acute febrile patients (n = 181) and a high risk group of humans, mainly slaughter workers (n = 38), were collected in the summer of 2014 and analyzed ...201626956221
ap92-like crimean-congo hemorrhagic fever virus in hyalomma aegyptium ticks, algeria. 026812469
candidatus coxiella massiliensis infection.bacteria genetically related to coxiella burnetii have been found in ticks. using molecular techniques, we detected coxiella-like bacteria, here named candidatus coxiella massiliensis, in skin biopsy samples and ticks removed from patients with an eschar. this organism may be a common agent of scalp eschar and neck lymphadenopathy after tick bite.201626811945
detection and phylogenetic characterization of theileria spp. and anaplasma marginale in rhipicephalus bursa in portugal.ticks are obligatory blood-sucking arthropod (acari:ixodida) ectoparasites of domestic and wild animals as well as humans. the incidence of tick-borne diseases is rising worldwide, challenging our approach toward diagnosis, treatment and control options. rhipicephalus bursa canestrini and fanzago, 1877, a two-host tick widely distributed in the palearctic mediterranean region, is considered a multi-host tick that can be commonly found on sheep, goats and cattle, and occasionally on horses, dogs, ...201626797395
detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria.in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria.201626791781
molecular detection and identification of spotted fever group rickettsiae in ticks collected from the west bank, palestinian territories.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group (sfg) rickettsiae. although spotted fever is prevalent in the middle east, no reports for the presence of tick-borne pathogens are available or any studies on the epidemiology of this disease in the west bank. we aimed to identify the circulating hard tick vectors and genetically characterize sfg rickettsia species in ixodid ticks from the west bank-palestinian territories.201626771654
functional analysis of esterase tce2 gene from tetranychus cinnabarinus (boisduval) involved in acaricide resistance.the carmine spider mite, tetranychus cinnabarinus is an important pest of crops and vegetables worldwide, and it has the ability to develop resistance against acaricides rapidly. our previous study identified an esterase gene (designated tce2) over-expressed in resistant mites. to investigate this gene's function in resistance, the expression levels of tce2 in susceptible, abamectin-, fenpropathrin-, and cyflumetofen-resistant strains were knocked down (65.02%, 63.14%, 57.82%, and 63.99%, respec ...201626725309
long term study of ixodid ticks feeding on red deer (cervus elaphus) in a meso-mediterranean climate.red deer (cervus elaphus l.) are very valuable in trophy-hunting but also contribute to the preservation of natural areas. they are affected by many parasites and pathogens, including hard ticks that are not only important parasites themselves but can also act as vectors and/or reservoirs of pathogens. tick phenology is complex insofar as population dynamics depend on environmental conditions, vegetation, host availability and their own intrinsic characteristic. ticks were collected monthly from ...201626715543
determination of prevalence and risk factors of infection with babesia ovis in small ruminants from west azerbaijan province, iran by polymerase chain reaction.small ruminants' babesiosis caused by babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. this study was carried out to to estimate the infection rate of b. ovis in sheep and goats by pcr. we have analysed risk factors that might influence infection of sheep and goats with b. ovis.201526623436
vectors of crimean congo hemorrhagic fever virus in iran.ticks are important vectors and reservoirs of crimean congo hemorrhagic fever (cchf) virus. human beings may be infected whenever the normal life cycle of the infected ticks on non-human vertebrate hosts is interrupted by the undesirable presence of humans in the cycle. a total of 26 species of argasid and ixodid ticks have been recorded in iran; including nine hyalomma, two rhipicephalus, two dermacentor, five haemaphysalis, two boophilus, one ixodes and two argas as well as three ornithodoros ...201526623426
evaluation of prognostic values of tissue plasminogen activator and plasminogen activator inhibitor-1 in crimean-congo hemorrhagic fever patients.crimean-congo hemorrhagic fever (cchf) is a widespread disease in turkey, and was responsible for many deaths in endemic regions during the last decade. the pathogenesis of the disease is not fully understood yet.201526587219
long-term ecological study of host-seeking adults of hyalomma lusitanicum (acari: ixodidae) in a meso-mediterranean climate.from january 2007 to december 2014, three representative meso-mediterranean bioclimatic environment types were sampled monthly using blanket-dragging techniques to determine the tick abundance rate. hyalomma lusitanicum koch, 1844 was the most prevalent species (96.58%) followed by dermacentor marginatus sulzer, 1776; rhipicephalus pusillus gil collado, 1936; and rhipicephalus bursa canestrini and fanzago, 1878. h. lusitanicum adults begin questing activity around march, numbers rising quickly r ...201626477051
flying ticks: anciently evolved associations that constitute a risk of infectious disease spread.ticks are important vectors of emerging zoonotic diseases affecting human and animal health worldwide. ticks are often found on wild birds, which have been long recognized as a potential risk factor for dissemination of ticks and tick-borne pathogens (tbp), thus raising societal concerns and prompting research into their biology and ecology. to fully understand the role of birds in disseminating some ticks species and tbp, it is important to consider the evolutionary relationships between birds, ...201526467109
coxiella-like endosymbiont associated to the "anatolian brown tick" rhipicephalus bursa in southern italy.several different ticks have been reported to harbor microbes related to coxiella burnetii, the agent of the q fever. rhipicephalus bursa is an important vector of tick-borne diseases in livestock in mediterranean area; it is also abundant in ovi-caprine farms with c. burnetii infection, in southern italy. 60 females of rh. bursa (15 pools) and 40 their eggs (2 pools) were screened for c. burnetii by a conventional pcr targeting the insertion sequence is1111 and by loop mediated isothermal ampli ...201626428855
development of a pan-babesia fret-qpcr and a survey of livestock from five caribbean islands.babesia spp. are tick-borne protozoan hemoparasites and the second most common blood-borne parasites of mammals, in particular domestic animals. we used the clustal multiple alignment program and 18s rrna gene sequences of 22 babesia species from genbank to develop a pcr that could detect a wide variety of babesia spp. in a single reaction. the pan-babesia fret-qpcr we developed reliably detected b. gibsoni, b. canis, b. vogeli, b. microti, b. bovis, and b. divergens under controlled conditions ...201526423577
molecular detection of babesia ovis in sheep and ticks using the gene encoding b. ovis surface protein d (bospd).the gene encoding babesia ovis surface protein d (bospd) was cloned from b. ovis cdna library. this gene encodes a polypeptide chain of 155 amino acids, including a predicted 22 amino acid signal peptide. sequence analysis of the bospd suggested that it is a surface protein with no known domains. blast analysis followed by multiple alignments showed four orthologs from other apicomplexan species and suggested that bospd is specific for b. ovis. bospd-based pcr was then developed to specifically ...201526391822
climate suitability for european ticks: assessing species distribution models against null models and projection under ar5 climate.there is increasing evidence that the geographic distribution of tick species is changing. whilst correlative species distribution models (sdms) have been used to predict areas that are potentially suitable for ticks, models have often been assessed without due consideration for spatial patterns in the data that may inflate the influence of predictor variables on species distributions. this study used null models to rigorously evaluate the role of climate and the potential for climate change to ...201526310856
external morphological anomalies in ixodid ticks from thrace, turkey.of 18,667 ticks examined, 33 specimens from species identified as haemaphysalis parva, hyalomma marginatum, hy. scupense, rhipicephalus bursa and rh. turanicus were found to have external morphological anomalies. anomalous ha. parva, hy. scupence and rh. turanicus were reported in this study for the first time. general anomalies manifested as asymmetry and deformations of the idiosoma, whereas local anomalies occurred in legs, exoskeleton, spiracular, adanal, subanal and accessory plates, mouthp ...201526264155
transmission of babesia ovis by different rhipicephalus bursa developmental stages and infected blood injection.in this report, the transmission efficacy of babesia ovis, the principal causative agent of ovine babesiosis, was studied by infestation of lambs with different rhipicephalus bursa stages or by injection of infected blood. infected blood injection induced acute babesiosis in splenectomized lambs, while only mild clinical signs were observed in intact animals. both splenectomized and intact lambs developed high antibody titer, detectable for at least 180 days post infection. infestation of splene ...201626253782
anaplasma infection in ticks, livestock and human in ghaemshahr, mazandaran province, iran.anaplasmosis is an important issue for animal breeders in terms of economic losses as well as a health concern to human. ticks are considered as the main vector of this disease. lack of documented information about anaplasma species in iran was the scope of this study to determine the population of ticks and the presence of anaplasma in ticks, domestic ruminants and also human beings in northern iran.201426114134
[distribution of tick species spread in kütahya vicinity].this study was performed to determine the tick species infested to the cattle and small ruminants in kütahya province and their seasonal activity and prevalence.201526081885
identification of tick species and disseminate pathogen using hemolymph by maldi-tof ms.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. the challenges of the present study were to assess maldi-tof ms profiling for the both identification of tick species and rickettsia spp. in infected ticks using hemolymph as protein mixture.201526051210
the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii.q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ...201525978383
preliminary investigation on tick fauna in the neighborhood of tarquinia, lazio, italy.this study represents a preliminary inquiry on tick fauna composition carried out in maremma, central italy, where very few data are available.201525857387
hard ticks (ixodidae) and crimean-congo hemorrhagic fever virus in south west of iran.ticks are vectors of some important arthropod-borne diseases in both fields of veterinary and medicine, such as lyme, tularemia, rocky mountain spotted fever, and some types of encephalitis as well as crimean congo hemorrhagic fever (cchf). iran is known as one of the main foci of cchf in west of asia. this study was conducted in darrehshahr county because of the development of animal husbandry in this area to detect the fauna and viral infection of the hard ticks of livestock. a cross-sectional ...201525796025
ectoparasite load in the crested porcupine hystrix cristata linnaeus, 1758 in central italy.the crested porcupine hystrix cristata is a large body-sized rodent, occurring in europe only in the italian peninsula, where it may have been introduced in early medieval times. its parasite fauna is currently poorly known and limited to few anecdotal observations. we have analyzed the ectoparasite load of 165 crested porcupines from tuscany and latium (central italy). both captured and road-killed individuals were checked for fleas and ticks. overall, only 39 porcupines were infested by four s ...201525773184
circulation of crimean-congo hemorrhagic fever virus in the former yugoslav republic of macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay.there are only few assays available for the detection of crimean-congo hemorrhagic fever virus (cchfv)-specific antibodies in animals, and data about diagnostic sensitivity and specificity are incompletely documented for most of these tests. this is unfortunate since cchfv antibodies in animals can be used as indicator for virus circulation in a geographic area and therewith potential risk of human exposure. this paper therefore reports on a novel elisa for the detection of cchfv-specific antibo ...201525742017
prevalence of ixodid ticks on cattle, sheep and goats in ilam county, ilam province, iran.this survey was performed to find out the infestation rate of ixodidae ticks in domestic ruminants in ilam county during 21 march 2009 to 23 august 2009. sampling was performed in 25 villages and 15 animal farm from different areas of this county. a total of 1,316 ticks were collected from 416 cattle, 208 sheep and 147 goats. the overall prevalence of ticks was recorded: 43, 23.5, and 49/6 % in cattle, sheep, and goats respectively. the number of ticks that collected from cattle, sheep, and goat ...201325698857
molecular epizootiology and diagnosis of porcine babesiosis in sardinia, italy.the recent characterization of the 18s ribosomal rna (rrna) of a pathogenic babesia species in a domestic sow paved the way for establishing diagnostic and epidemiological tools for porcine babesiosis. here, we developed the first specific babesia sp. suis pcr, and we applied this test to a panel of samples collected from animals living in a typical mediterranean environment (sardinia, italy), including domestic pigs, wild boars, and ticks. in domestic pigs, pcr coupled with sequencing revealed ...201425325315
molecular identification of theileria and babesia in ticks collected from sheep and goats in the black sea region of turkey.a molecular survey was undertaken in the black sea region of turkey to determine the presence of theileria and babesia species of medical and veterinary importance. the ticks were removed from sheep and goats, pooled according to species and locations, and analyzed by pcr-based reverse line blot (rlb) and sequencing. a total of 2241 ixodid ticks belonging to 5 genus and 12 species were collected and divided into 310 pools. infection rates were calculated as the maximum likelihood estimation (mle ...201525260692
crimean-congo hemorrhagic fever virus clades v and vi (europe 1 and 2) in ticks in kosovo, 2012.despite being a small country, kosovo represents one of the few foci of crimean-congo hemorrhagic fever (cchf) in europe. the distribution of kosovar tick vectors and the evolution of cchf virus in ticks are both as yet unknown. a better description of the extent and the genetic diversity of cchfv in ticks from endemic settings is essential, in order to be controlled. we investigated the 2012 distribution of kosovar ticks alongside the prevalence and the phylogeography of tick-derived cchfv. hya ...201425255381
a new multiple-locus variable-number tandem repeat analysis reveals different clusters for anaplasma phagocytophilum circulating in domestic and wild ruminants.anaplasma phagocytophilum is a tick-borne intragranulocytic alpha-proteobacterium. it is the causative agent of tick-borne fever in ruminants, and of human granulocytic anaplasmosis in humans, two diseases which are becoming increasingly recognized in europe and the usa. however, while several molecular typing tools have been developed over the last years, few of them are appropriate for in-depth exploration of the epidemiological cycle of this bacterium. therefore we have developed a multiple-l ...201425228371
distribution of ticks (acari: ixodidae) infesting domestic ruminants in mountainous areas of golestan province, iran.to determine the prevalence of ticks on cattle in the mountainous areas of golestan province and their geographical distribution.025183090
survey on cattle ticks in nur, north of iran.to survey the prevalence of cattle ticks in nur county and prepare a list of tick fauna in this district.201425182439
identification of tick-borne pathogens in ticks feeding on humans in turkey.the importance of tick-borne diseases is increasing all over the world, including turkey. the tick-borne disease outbreaks reported in recent years and the abundance of tick species and the existence of suitable habitats increase the importance of studies related to the epidemiology of ticks and tick-borne pathogens in turkey. the aim of this study was to investigate the presence of and to determine the infection rates of some tick-borne pathogens, including babesia spp., borrelia burgdorferi se ...201425101999
role of migratory birds in spreading crimean-congo hemorrhagic fever, turkey.we investigated migratory birds' role in spreading crimean-congo hemorrhagic fever virus (cchfv) through attached ticks. we detected cchfv rna in ticks on migratory birds in turkey. two isolates showed similarity with cchfv genotype 4, suggesting a role for ticks in cchfv epidemics in turkey and spread of cchfv by birds.025062428
prevalence of crimean-congo hemorrhagic fever virus antibodies in greek residents in the area where the ap92 strain was isolated.crimean-congo hemorrhagic fever (cchf) is a viral disease with fatality rate up to 30%. up to date, only one cchf case has been reported in greece, while a distinct virus strain (ap92) had been isolated in 1975 from ticks in imathia prefecture, northern greece. the aim of the present study was to estimate the cchf virus (cchfv) seroprevalence among humans residing in the prefecture of imathia, and the neighboring prefecture of pella, and to investigate the risk factors associated with the seropo ...025031510
ticks infesting humans in italy and associated pathogens.ticks may transmit a large variety of pathogens, which cause illnesses in animals and humans, commonly referred to as to tick-borne diseases (tbds). the incidence of human tbds in italy is underestimated because of poor surveillance and the scant amount of studies available.201425023709
spotted fever rickettsia species in hyalomma and ixodes ticks infesting migratory birds in the european mediterranean area.a few billion birds migrate annually between their breeding grounds in europe and their wintering grounds in africa. many bird species are tick-infested, and as a result of their innate migratory behavior, they contribute significantly to the geographic distribution of pathogens, including spotted fever rickettsiae. the aim of the present study was to characterize, in samples from two consecutive years, the potential role of migrant birds captured in europe as disseminators of rickettsia-infecte ...201425011617
molecular evidence for trans-stadial and transovarial transmission of babesia occultans in hyalomma marginatum and rhipicephalus turanicus in turkey.a total of 1064 adult ticks identified as hyalomma marginatum (n=965), rhipicephalus turanicus (n=86), haemaphysalis inermis (n=9) and rhipicephalus bursa (n=4) along with 20 egg masses derived from h. marginatum (n=18) and r. bursa were screened for babesia occultans. the ticks were combined into 328 pools and infection rates were calculated as the maximum likelihood estimation (mle) with 95% confidence intervals (ci). of these pools, 23 (7%) were positive for b. occultans, and the overall mle ...201425002306
a 24-48 h fed amblyomma americanum tick saliva immuno-proteome.multiple tick saliva proteins, the majority of which are unknown, confer tick resistance in repeatedly infested animals. the objective of this study was to identify the 24-48 h fed amblyomma americanum tick saliva immuno-proteome. the 24-48 h tick-feeding phase is critical to tick parasitism as it precedes important events in tick biology, blood meal feeding and disease agent transmission. fed male, 24 and 96 h fed female phage display cdna expression libraries were biopanned using rabbit antibo ...201424962723
a novel ap92-like crimean-congo hemorrhagic fever virus strain, greece.ticks were collected from various regions of northern greece and tested for the presence of crimean-congo hemorrhagic fever virus (cchfv) rna. human and animal sera were collected in the regions where cchfv-positive ticks were detected, and they were tested for the presence of igg antibodies against the virus. a cchfv strain was detected in rhipicephalus bursa ticks collected from sheep in kastoria regional unit, differing by 9.7% at the nucleotide level from the ap92 strain, which was isolated ...201424953797
pcr-based detection of babesia ovis in rhipicephalus bursa and small ruminants.this study aimed to assess the prevalence of babesia ovis infection in adult rhipicephalus bursa and small ruminants in west azerbaijan province, iran. blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. specific b. ovis fragment was detected in 67 animals (16.7%), of which 52 animals (18.6%) were sheep and 15 animals (12.2%) goats (p < 0.05). of the 848 r. bursa collected from naturally infested small ruminants and farm dogs, babesia ovis was detected by ...201424876944
prevalence of piroplasms in small ruminants in north-west tunisia and the first genetic characterisation of babesia ovis in africa.in this study, the prevalence of piroplasms in sheep and goats was assessed with giemsa-stained blood smear examination, pcr and nested pcr-restriction fragment length polymorphism (rflp) to identify babesia and theileria species, respectively, in 338 small ruminants (172 sheep and 166 goats) from three sites in north-west tunisia during the 2011 summer season. the overall infection prevalence of piroplasms in giemsa-stained blood smears was 3.2% (11/338), with a parasitaemia ranging from 0.01 t ...201424849588
retrospective study of hemoparasites in cattle in southern italy by reverse line blot hybridization.tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. in order to assess the presence and prevalence of various pathogens in southern italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (rlb) hybridization. the study had been carried out in three regions of southern italy on 1,500 randomly selected and apparently healthy adult cattle. rlb showed that ...201424614604
molecular and serological detection of theileria equi and babesia caballi infection in horses and ixodid ticks in iran.equine piroplasmosis is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by theileria equi and babesia caballi. however, the geographical distribution of equine piroplasmosis in iran is unknown. the aim of the current study was to determine the causative agents and vector ticks of equine piroplasmosis in horses in the north khorasan province. in the year 2011, 100 horses were randomly selected from 14 villages. blood samples and ixodid ticks were collected and examin ...201424556274
assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex dna bead-based suspension array.piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. the purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the basque country (nort ...201324499621
hard tick factors implicated in pathogen transmission.ticks are the most common arthropod vector, after mosquitoes, and are capable of transmitting the greatest variety of pathogens. for both humans and animals, the worldwide emergence or re-emergence of tick-borne disease is becoming increasingly problematic. despite being such an important issue, our knowledge of pathogen transmission by ticks is incomplete. several recent studies, reviewed here, have reported that the expression of some tick factors can be modulated in response to pathogen infec ...201424498444
crimean-congo hemorrhagic fever virus, greece.seroprevalence of crimean-congo hemorrhagic fever virus (cchfv) is high in some regions of greece, but only 1 case of disease has been reported. we used 4 methods to test 118 serum samples that were positive for cchfv igg by commercial elisa and confirmed the positive results. a nonpathogenic or low-pathogenicity strain may be circulating.024447877
a survey of ixodid tick species and molecular identification of tick-borne pathogens.this study was undertaken in two different climatic areas of turkey to determine the presence of tick-borne pathogens of medical and veterinary importance. the ticks were removed from humans, pooled according to species and developmental stages, and analyzed by pcr, reverse line blot (rlb) and sequencing. of the 2333 removed ticks from 10 species, 1238 (53.06%) were obtained from the arid cold zone, and the remaining 1095 (46.93%) were obtained from the humid zone. the removed ticks were identif ...201424424312
detection of naturally infected vector ticks (acari: ixodidae) by different species of babesia and theileria agents from three different enzootic parts of iran.diagnostic study of vector ticks for different pathogens transmitted specifically have been done by iranian old scientists working on the basis of biological transmission of pathogens. in this study we decided to confirm natural infection of different collected ticks from three different provinces of iran.201324409442
control of tick infestations and pathogen prevalence in cattle and sheep farms vaccinated with the recombinant subolesin-major surface protein 1a chimeric antigen.despite the use of chemical acaricides, tick infestations continue to affect animal health and production worldwide. tick vaccines have been proposed as a cost-effective and environmentally friendly alternative for tick control. vaccination with the candidate tick protective antigen, subolesin (sub), has been shown experimentally to be effective in controlling vector infestations and pathogen infection. furthermore, escherichia coli membranes containing the chimeric antigen composed of sub fused ...201424398155
[molecular-genetic characterization of the bhanja virus (bhav) and the razdan virus (razv) (bunyaviridae, phlebovirus) isolated from the ixodes ticks rhipicephalus bursa (canestrini and fanzago, 1878) and dermacentor marginatus (sulzer, 1776) in transcaucasus].two bunyaviruses, bhanja (bhav, leiv-az1818) isolated from the ixodes ticks rhipecephalus bursa (canestrini and fanzago, 1878) in azerbaijan (1973) and razdan (razv; strain leiv-arm2741) isolated from the dermacentor marginatus (sulzer, 1776) ticks in armenia (1972), were de novo sequenced (on the illumina platform). the amino acid identity between these viruses proteins were 95.8% (rdrp, l-segment), 90.3% (gngc, m-segment), and 92.5% (n, s-segment). thus, razv was classified to bhav group. gngc ...201624354060
molecular and serological evidence for the circulation of the tick symbiont midichloria (rickettsiales: midichloriaceae) in different mammalian species.the midichloriaceae is a novel family of the order rickettsiales, that encompasses intracellular bacteria associated with hard ticks (ixodidae) and other arthropods. the most intensively investigated member of this family is midichloria mitochondrii, a symbiotic bacterium of the sheep tick ixodes ricinus, characterized by the capacity of multiplying inside the mitochondria. a recent study suggested that these bacteria might be inoculated into the human host during the tick bite. the purpose of t ...201324330701
overexpression of multiple detoxification genes in deltamethrin resistant laodelphax striatellus (hemiptera: delphacidae) in china.the small brown planthopper (sbph), laodelphax striatellus (fallén), is one of the major rice pests in asia and has developed resistance to multiple classes of insecticides. understanding resistance mechanisms is essential to the management of this pest. biochemical and molecular assays were performed in this study to systematically characterize deltamethrin resistance mechanisms with laboratory-selected resistant and susceptible strains of sbph.201324324548
detection of rickettsia in rhipicephalus sanguineus ticks and ctenocephalides felis fleas from southeastern tunisia by reverse line blot assay.ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern tunisia were screened for rickettsia infection using reverse line blot assay. evidence of spotted fever group rickettsia was obtained. we detected rickettsia felis in fleas, rickettsia massiliae bar 29 and the rickettsia conorii israeli spotted fever strain in ticks, and rickettsia conorii subsp. conorii and rickettsia spp. in both arthropods. the sensitivity of the adopted technique allowed the identification ...201324226919
seasonal dynamics of tick species in an urban park of rome.regular collections were obtained in the natural reserve of the insugherata of rome during 2011 in order to obtain the tick species composition and the respective seasonal dynamics of the area. a total of 325 ticks was collected in selected sites by means of drag sampling. among the identified species, rhipicephalus turanicus was the most abundant (72.3%), followed by ixodes ricinus (19.7%), dermacentor marginatus (6.5%), haemaphysalis punctata (1.2%), and rhipicephalus bursa (0.3%). r. turanicu ...201324140501
comparative analyses of mitochondrial and nuclear genetic markers for the molecular identification of rhipicephalus spp.the genus rhipicephalus (acari: ixodidae) comprises a large number of vectors of pathogens of substantial medical and veterinary concern; however, species identification based solely on morphological features is often challenging. in the present study, genetic distance within selected rhipicephalus species (i.e., rhipicephalus bursa, rhipicephalus guilhoni, rhipicephalus muhsamae, rhipicephalus sanguineus sensu lato and rhipicephalus turanicus), were investigated based on molecular and phylogene ...201324103336
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
comparative analysis of mitochondrial markers from four species of rhipicephalus (acari: ixodidae).mitochondrial sequences of four mitochondrial markers: 12s rrna, 16s rrna, cytochrome c oxidase subunit i (cox1) and cytochrome b (cytb) from four rhipicephalus species were analyzed to establish genetic relationships and enable molecular identification. field-collected samples from the species rhipicephalus annulatus, rhipicephalus bursa, rhipicephalus sanguineus and rhipicephalus turanicus were amplified by pcr and compared with genbank™ annotated sequences. pcr products were obtained using pr ...201324091196
prevalence of ixodid tick infestation of sheep in the arasbaran region of iran.this survey was carried out in sheep herds of arasbaran region in northwest of iran. the aims of this survey were assessment of occurrence hard ticks on sheep in this region and speciation and identification of detached ticks from sheep. in addition determination of tick distribution on sheep body surface was studied. in this study 330 sheep were examined and ticks from infested animals were collected. in parasitological laboratory speciation of ticks were done by stereomicroscope and using of t ...201224082534
a molecular study of tick-borne haemoprotozoan parasites (theileria and babesia) in small ruminants in northern tunisia.in this study, the frequency of theileria and babesia species in sheep and goats was assessed via reverse line blotting (rlb). a total of 263 apparently healthy sheep and goats, from 16 randomly selected flocks located in 9 localities situated in 3 bioclimatic zones in tunisia, were investigated for the blood protozoans. rlb hybridization with polymerase chain reaction detected only theileria ovis in sheep and goats, accounting for 22.4% (95% confidence interval [ci]: 17.6-27.1%) positive sample ...201324029713
mediterranean spotted fever in southeastern romania.although cases of mediterranean spotted fever (msf) have been reported for decades in southeastern romania, there are few published data. we retrospectively studied 339 patients, diagnosed with msf at the national institute of infectious diseases "prof. dr. matei bals" between 2000 and 2011, in order to raise awareness about msf in certain regions of romania. according to the raoult diagnostic criteria 171 (50.4%) had a score >25 points. mean age was 52.5 years. one hundred and fifty-five (90.6% ...201324024190
Displaying items 1 - 100 of 255