Publications

rice grassy stunt virus p5 interacts with two protein components of the plant-specific cbl-cipk ca(+2) signaling network of rice.	rice grassy stunt virus (rgsv) is a tenuivirus posing a threat to rice production in many south, southeast, and east asian countries. to date, no host factor interacting with rgsv has been reported. in this study, we screened a rice cdna library with the gal4-based yeast two-hybrid system using rgsv p5 as the bait. one of the candidate host factors interacting with rgsv p5 was found to be cbl-interacting protein kinase 25 (oscipk25), a member of the plant-specific cbl-cipk ca(2+) signaling netwo ...	2017	28213698
inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of rice stripe virus.	two tenuiviruses rice stripe virus (rsv) and rice grassy stunt virus (rgsv) were found to co-infect rice with the same reovirus rice ragged stunt virus (rrsv). during the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-rna leaders from the rrsv. a total of 245 and 102 rrsv-rgsv and rrsv-rsv chimeric mrna clones, respectively, were sequenced. an analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped lead ...	2016	27393974
nac transcription factor family genes are differentially expressed in rice during infections with rice dwarf virus, rice black-streaked dwarf virus, rice grassy stunt virus, rice ragged stunt virus, and rice transitory yellowing virus.	expression levels of the nac gene family were studied in rice infected with rice dwarf virus (rdv), rice black-streaked dwarf virus (rbsdv), rice grassy stunt virus (rgsv), rice ragged stunt virus (rrsv), and rice transitory yellowing virus (rtyv). microarray analysis showed that 75 (68%) osnac genes were differentially regulated during infection with rdv, rbsdv, rgsv, and rrsv compared with the control. the number of osnac genes up-regulated was highest during rgsv infection, while the lowest n ...	2015	26442000
rice responses and resistance to planthopper-borne viruses at transcriptomic and proteomic levels.	rice (oryza sativa) is one of the most important cereal crops in the world, especially in asian areas. rice virus diseases are considered as the most serious threat to rice yields. most rice viruses are transmitted by hemipteran insects such as planthoppers and leafhoppers. in asia five rice viruses are transmitted mainly by three planthopper species in a persistent manner: rice stripe virus, rice black-streaked dwarf virus, rice ragged stunt virus, rice grassy stunt virus, and southern rice bla ...	2016	26363817
rice grassy stunt virus nonstructural protein p5 serves as a viral suppressor of rna silencing and interacts with nonstructural protein p3.	rice grassy stunt virus (rgsv), a member of the genus tenuivirus, causes serious rice disease in southeast asian countries. in this study, a green fluorescent protein (gfp)-based transient expression assay was conducted to show that p5, encoded on rna5 in the viral sense, is a viral suppressor of rna silencing (vsr). protein-protein interactions (ppis) between p5 and all rgsv proteins except pc1 and pc2 were investigated using gal4-based yeast two-hybrid (y2h) experiments. the results demonstrat ...	2015	26296721
p2 of rice grassy stunt virus (rgsv) and p6 and p9 of rice ragged stunt virus (rrsv) isolates from vietnam exert suppressor activity on the rna silencing pathway.	in vietnam, the two main viruses that cause disease in rice are the rice grassy stunt virus (rgsv) and the rice ragged stunt virus (rrsv). outbreaks of these two viruses have dramatically decreased rice production in vietnam. because natural resistance genes are unknown, an rnai strategy may be an alternative method to develop resistance to rgsv and rrsv. however, this strategy will be efficient only if putative silencing suppressors encoded by the two viruses are neutralized. to identify these ...	2015	26215087
functional comparison of rna silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus.	rice grassy stunt virus (rgsv) is a member of the genus tenuivirus, which includes rice stripe virus (rsv), as the type species. a viral suppressor of rna silencing (vsr) of rgsv has not been identified, whereas the p3 protein of rsv (rsvp3) encoded by the viral-sense (v) strand of rna3 has been reported to act as a vsr. in this study, we examined the vsr function of the p5 protein of rgsv (rgsvp5), encoded by vrna5. expecting it to correspond to the vrna3 of rsv, we compared the vsr function of ...	2015	25836276
infection route of rice grassy stunt virus, a tenuivirus, in the body of its brown planthopper vector, nilaparvata lugens (hemiptera: delphacidae) after ingestion of virus.	rice grassy stunt virus (rgsv), a tenuivirus, is transmitted by the brown planthopper (bph), nilaparvata lugens (hemiptera, delphacidae), in a persistent-propagative manner. in this study, immunofluorescence microscopy was used to investigate the infection route of rgsv in the internal organs of bph after acquiring the virus by feeding on rgsv-infected rice plants. the sequential infection study revealed that rgsv initially infected the midgut epithelium, then crossed the basal lamina into the m ...	2014	24768845
development of continuous cell culture of brown planthopper to trace the early infection process of oryzaviruses in insect vector cells.	rice ragged stunt virus (rrsv), an oryzavirus in the family reoviridae, is transmitted by the brown planthopper, nilaparvata lugens, in a persistent-propagative manner. here, we established a continuous cell line of brown planthopper to investigate the mechanism underlying the formation of the viroplasm, the putative site for viral replication and assembly, during infection of rrsv in its insect vector cells. within 24 h of viral infection of cultured cells, the viroplasm had formed and containe ...	2014	24478421
relationship between gene responses and symptoms induced by rice grassy stunt virus.	rice grassy stunt virus (rgsv) is a serious threat to rice production in southeast asia. rgsv is a member of the genus tenuivirus, and it induces leaf yellowing, stunting, and excess tillering on rice plants. here we examined gene responses of rice to rgsv infection to gain insight into the gene responses which might be associated with the disease symptoms. the results indicated that (1) many genes related to cell wall synthesis and chlorophyll synthesis were predominantly suppressed by rgsv inf ...	2013	24151491
strong resistance against rice grassy stunt virus is induced in transgenic rice plants expressing double-stranded rna of the viral genes for nucleocapsid or movement proteins as targets for rna interference.	rice grassy stunt virus (rgsv), a member of the genus tenuivirus, causes significant economic losses in rice production in south, southeast, and east asian countries. growing resistant varieties is the most efficient method to control rgsv; however, suitable resistance genes have not yet been found in natural rice resources. one of the most promising methods to confer resistance against rgsv is the use of rna interference (rnai). it is important to target viral genes that play important roles in ...	2013	23190115
molecular diversity of rice grassy stunt virus in vietnam.	rice grassy stunt virus (rgsv, tenuivirus) recently emerged on rice in vietnam, causing high yield losses during 2006-2009. the genetic diversity of rgsv is poorly documented. in this study, the two genes encoded by each ambisense segment rna3 and rna5 of rgsv isolates from six provinces of south vietnam were sequenced. p3 and pc3 (rna3) have unknown function, p5 (rna5) encodes the putative silencing suppressor, and pc5 (rna5) encodes the nucleocapsid protein (n). the sequences of 17 vietnamese ...	2013	23132204
the nonstructural protein pc6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus.	the nonstructural protein pc6 encoded by rice grassy stunt virus is thought to correspond functionally to the nonstructural protein pc4 of rice stripe virus, which can support viral cell-to-cell movement. in a trans-complementation experiment with a movement-defective tomato mosaic virus, pc6 and pc4 facilitated intercellular transport of the virus. transient expression of pc6, fused with green fluorescent protein, in epidermal cells was predominantly observed close to the cell wall as well as i ...	2011	21327784
molecular detection of nine rice viruses by a reverse-transcription loop-mediated isothermal amplification assay.	a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay was established for the detection of nine viruses from infected rice plants, including rice black-streaked dwarf virus (rbsdv), rice dwarf virus (rdv), rice gall dwarf virus (rgdv), rice ragged stunt virus (rrsv), rice transitory yellowing virus (rtyv), rice stripe virus (rsv), rice grassy stunt virus (rgsv), rice tungro spherical virus (rtsv), and rice tungro bacilliform virus (rtbv). virus-specific primer sets were ...	2010	20837064
detection of rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers.	the genome of rice grassy stunt virus (rgsv) consists of 6 ambisense rna segments, among which rnas 1, 2, 5 and 6 are equivalent to rnas 1, 2, 3 and 4, respectively, of rice stripe virus, the type species of the genus tenuivirus. the rgsv 36-kda nucleocapsid protein (n) is encoded on the complementary strand of rna 5. here, we studied accumulation of three nonstructural proteins, a 23-kda p2 protein encoded on vrna 2 (virus genomic strand), a 22-kda p5 protein encoded on vrna 5, and a 21-kda p6 ...	2002	12491098
nucleotide sequence of a dianthovirus rna1-like rna found in grassy stunt-diseased rice plants.	a dianthovirus rna1-like rna (dr1l rna, 4486 nucleotides in length) was found in grassy stunt-diseased rice plants together with rice grassy stunt virus (rgsv). dr1l rna has characteristics common to dianthovirus rna1 such as (1) presence of a ggauuuuuag potential shifty-heptanucleotide at the end of the 5'-proximal orf, which encodes a 35-kda protein, followed by a 77-nucleotide sequence capable of forming a stem-loop structure for an efficient--1 frameshift to express the downstream region in ...	2001	11315634
comparison of nucleotide sequences between northern and southern philippine isolates of rice grassy stunt virus indicates occurrence of natural genetic reassortment.	rice grassy stunt virus is a member of the genus tenuivirus, is persistently transmitted by a brown planthopper, and has occurred in rice plants in south, southeast, and east asia [corrected]. we determined the complete nucleotide (nt) sequences of rnas 1 (9760 nt), 2 (4069 nt), 3 (3127 nt), 4 (2909 nt), 5 (2704 nt), and 6 (2590 nt) of a southern philippine isolate from south cotabato and compared them with those of a northern philippine isolate from laguna (toriyama et al., 1997, 1998). the num ...	2000	10612657
the complete nucleotide sequence of the rice grassy stunt virus genome and genomic comparisons with viruses of the genus tenuivirus.	rice grassy stunt virus (rgsv, irri isolate) has six genomic rna segments. the nucleotide (nt) sequences of rnas 1-4 were determined. the cumulative length of the rgsv genome, including rnas 5 and 6, was 25142 nt. all six rna segments had an ambisense coding strategy and almost identical terminal sequences over 17 nt. the virus complementary (vc) sequence of the largest segment, rna1, had an open reading frame encoding a protein of mr 339133 (the 339.1k protein), while the virus sense (v) sequen ...	1998	9714257
the proteins encoded by rice grassy stunt virus rna5 and rna6 are only distantly related to the corresponding proteins of other members of the genus tenuivirus.	the genome of rice grassy stunt virus (rgsv) consists of six rna segments. the nucleotide (nt) sequences of the two smallest segments, rnas 5 and 6, were determined and found to comprise 2704 and 2584 nt, respectively. the 5'- and 3'-terminal sequences of both rnas were identical over a length of 21 nt and could potentially form a panhandle-like structure due to intramolecular complementarity. each rna segment contained a virus (v) sense open reading frame (orf) in the 5'-proximate region, and a ...	1997	9292026