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fverga1, encoding a della protein, negatively regulates runner production in fragaria vesca.fverga1 was highly expressed in tender tissues such as young leaves and stem apices and was localized in the nucleus. rnai silencing of fverga1 in non-runnering woodland strawberry produced many runners. fverga1 is thus a key gene controlling strawberry runner formation. the propagation of strawberry is mainly based on runners, while the genes controlling runner production have not been well characterized. exogenous applications of optimum concentration gibberellins (gas) promote runner formatio ...201729288326
integrated karyotyping of woodland strawberry (fragaria vesca) with oligopaint fish probes.chromosome identification is critical for many aspects of cytogenetic research. however, for fragaria vesca, definite identification of individual chromosomes is almost impossible because of their small size and high similarity. here, we demonstrate that bulked oligonucleotide (oligo) probes can be used as chromosome-specific dna markers for chromosome identification in f. vesca. oligos specific to entire pseudochromosomes in the draft genome of f. vesca were identified and synthesized as librar ...201729262412
combination of lc-ms based metabolomics and antioxidant activity for evaluation of bioactive compounds in fragaria vesca leaves from italy.wild strawberry (fragaria vesca) is recognized as a traditional food of campania region (italy). the vegetative parts have a pharmaceutical interest, being used as decoctions for hypertension treatment due their detoxifying, diuretic, stimulant and dermatological protective properties; leaves, that after collection of fruits are generally thrown away, could be an interesting byproduct, source of bioactive compounds for cosmetic and pharmaceutical industry. in the present work, the metabolite pro ...201829253779
single-molecule sequencing and optical mapping yields an improved genome of woodland strawberry (fragaria vesca) with chromosome-scale contiguity.although draft genomes are available for most agronomically important plant species, the majority are incomplete, highly fragmented, and often riddled with assembly and scaffolding errors. these assembly issues hinder advances in tool development for functional genomics and systems biology.201729253147
identification and validation of fap1d7, a putative marker associated with the biosynthesis of methyl butanoate in cultivated strawberry (fragaria x ananassa).breeding strawberry (fragaria x ananassa) with enhanced fruit flavour is one of the top breeding goals of many strawberry-producing countries. although several genes involved in the biosynthetic pathways of key aroma compounds have been identified, the development and application of molecular markers associated with fruit flavour remain limited. this study aims to identify molecular markers closely linked to genes controlling strawberry aroma. a purpose-built subtracted diversity array (sda) kno ...201729234071
the arabidopsis elp3/elo3 and elp4/elo1 genes enhance disease resistance in fragaria vesca l.plant immune response is associated with a large-scale transcriptional reprogramming, which is regulated by numerous transcription regulators such as the elongator complex. elongator is a multitasking protein complex involved in diverse cellular processes, including histone modification, dna methylation, and trna modification. in recent years, elongator is emerging as a key regulator of plant immune responses. however, characterization of elongator's function in plant immunity has been conducted ...201729191170
xths from fragaria vesca: genomic structure and transcriptomic analysis in ripening fruit and other tissues.fragaria vesca or 'woodland strawberry' has emerged as an attractive model for the study of ripening of non-climacteric fruit. it has several advantages, such as its small genome and its diploidy. the recent availability of the complete sequence of its genome opens the possibility for further analysis and its use as a reference species. fruit softening is a physiological event and involves many biochemical changes that take place at the final stages of fruit development; among them, the remodeli ...201729115918
genetic analysis of the wild strawberry (fragaria vesca) volatile composition.the volatile composition of wild strawberry (fragaria vesca) fruit differs from that of the cultivated strawberry, having more intense and fruity aromas. over the last few years, the diploid f. vesca has been recognized as a model species for genetic studies of cultivated strawberry (f. x ananassa), and here a previously developed f. vesca/f. bucharica near isogenic line collection (nil) was used to explore genetic variability of fruit quality traits. analysis of fruit volatiles by gc-ms in our ...201729100102
gene expression atlas of fruit ripening and transcriptome assembly from rna-seq data in octoploid strawberry (fragaria × ananassa).rna-seq has been used to perform global expression analysis of the achene and the receptacle at four stages of fruit ripening, and of the roots and leaves of strawberry (fragaria × ananassa). about 967 million reads and 191 gb of sequence were produced, using illumina sequencing. mapping the reads in the related genome of the wild diploid fragaria vesca revealed differences between the achene and receptacle development program, and reinforced the role played by ethylene in the ripening receptacl ...201729062051
fragaria vesca constans controls photoperiodic flowering and vegetative development.according to the external coincidence model, photoperiodic flowering occurs when constans (co) mrna expression coincides with light in the afternoon of long days (lds), leading to the activation of flowering locus t (ft). co has evolved in brassicaceae from other group ia co-like (col) proteins which do not control photoperiodic flowering in arabidopsis. cols in other species have evolved different functions as floral activators or even as repressors. to understand photoperiodic development in t ...201729048562
genome re-annotation of the wild strawberry fragaria vesca using extensive illumina- and smrt-based rna-seq datasets.the genome of the wild diploid strawberry species fragaria vesca, an ideal model system of cultivated strawberry (fragaria × ananassa, octoploid) and other rosaceae family crops, was first published in 2011 and followed by a new assembly (fvb). however, the annotation for fvb mainly relied on ab initio predictions and included only predicted coding sequences, therefore an improved annotation is highly desirable. here, a new annotation version named v2.0.a2 was created for the fvb genome by a pip ...201729036429
soft mechanical stimulation induces a defense response against botrytis cinerea in strawberry.genes associated with plant mechanical stimulation were found in strawberry genome. a soft mechanical stimulation (sms) induces molecular and biochemical changes in strawberry plants, conferring protection against botrytis cinerea. plants have the capacity to induce a defense response after exposure to abiotic stresses acquiring resistance towards pathogens. it was reported that when leaves of arabidopsis thaliana were wounded or treated with a soft mechanical stimulation (sms), they could resis ...201829032427
genome-wide characterization, evolution, and expression analysis of the leucine-rich repeat receptor-like protein kinase (lrr-rlk) gene family in rosaceae genomes.leucine-rich repeat receptor-like protein kinase (lrr-rlk) is the largest gene family of receptor-like protein kinases (rlks) and actively participates in regulating the growth, development, signal transduction, immunity, and stress responses of plants. however, the patterns of lrr-rlk gene family evolution in the five main rosaceae species for which genome sequences are available have not yet been reported. in this study, we performed a comprehensive analysis of lrr-rlk genes for five rosaceae ...201729017442
plant uptake of per- and polyfluoroalkyl substances at a contaminated fire training facility to evaluate the phytoremediation potential of various plant species.fire training facilities and other areas suffer from serious per- and polyfluoroalkyl substance (pfas) contamination in soil, surface water, and groundwater due to regular practices with pfas-containing aqueous firefighting foams (afffs). therefore, the uptake of 26 pfass in plants and the contamination of soil and groundwater has been investigated at a fire training site at stockholm arlanda airport, stockholm (sweden) in 2016. elevated ∑26pfas levels were detected in soil and groundwater rangi ...201728972370
patterns of low temperature induced accumulation of dehydrins in rosaceae crops-evidence for post-translational modification in apple.important crop plants of rosaceae family are often damaged during winter due to the lack of acclimation and cold hardiness. one of the cellular responses of plants to cold stress is the accumulation of dehydrin proteins. we studied the expression of dehydrins in several rosaceae species during low temperature treatment in vitro. microshoots of pyrus communis, malus×domestica, fragaria vesca, fragaria×ananassa, prunus cerasus and prunus avium cultivars were grown in low temperature conditions. ge ...201728886453
a specific gibberellin 20-oxidase dictates the flowering-runnering decision in diploid strawberry.asexual and sexual reproduction occur jointly in many angiosperms. stolons (elongated stems) are used for asexual reproduction in the crop species potato (solanum tuberosum) and strawberry (fragaria spp), where they produce tubers and clonal plants, respectively. in strawberry, stolon production is essential for vegetative propagation at the expense of fruit yield, but the underlying molecular mechanisms are unknown. here, we show that the stolon deficiency trait of the runnerless (r) natural mu ...201728874507
white-fruited strawberry genotypes are not per se hypoallergenic.the strawberry fruit fra a 1-proteins are homologues of the major birch pollen allergen bet v 1 and have essential biological functions in pigment formation during fruit ripening. patients affected by allergy against birch pollen tolerated fruits of a naturally occurring white-fruited f.×ananassa genotype, which showed reduced levels of fra a 1 proteins along with enzymes of the anthocyanin pigment pathway. we evaluated the cross-reactive allergenic potential of a number of naturally occurring w ...201728873746
population genetic analysis of a global collection of fragaria vesca using microsatellite markers.the woodland strawberry, fragaria vesca, holds great promise as a model organism. it not only represents the important rosaceae family that includes economically important species such as apples, pears, peaches and roses, but it also complements the well-known model organism arabidopsis thaliana in key areas such as perennial life cycle and the development of fleshy fruit. analysis of wild populations of a. thaliana has shed light on several important developmental pathways controlling, for exam ...201728854285
altered regulation of terminal flower 1 causes the unique vernalisation response in an arctic woodland strawberry accession.vernalisation requirement is an agriculturally important trait that postpones the development of cold-sensitive floral organs until the spring. the family rosaceae includes many agriculturally important fruit and berry crops that suffer from crop losses caused by frost injury to overwintering flower buds. recently, a vernalisation-requiring accession of the rosaceae model woodland strawberry (fragaria vesca) has been identified in northern norway. understanding the molecular basis of the vernali ...201728815698
identification and analysis of mitogen-activated protein kinase (mapk) cascades in fragaria vesca.mitogen-activated protein kinase (mapk) cascades are highly conserved signaling modules in eukaryotes, including yeasts, plants and animals. mapk cascades are responsible for protein phosphorylation during signal transduction events, and typically consist of three protein kinases: mapk, mapk kinase, and mapk kinase kinase. in this current study, we identified a total of 12 fvmapk, 7 fvmapkk, 73 fvmapkkk, and one fvmapkkkk genes in the recently published fragaria vesca genome sequence. this work ...201728805715
antidepressent effect of two new benzyl derivatives from wild strawberry fragaria vesca var. nubicola lindl. ex hook.f.two new benzyl derivatives were isolated from ethyl acetate fraction of wild strawberry, fragaria vesca var. nubicola lindl. ex hook.f. the structures of these compounds were elucidated to be 5-(4-hydroxy-3-methoxyphenethyl)-7-methoxy-2h-chromen-3-ol (1) and 5-(4-hydroxy-3-methoxyphenethyl)-4,7-dimethoxy-2h-chromen-3-ol (2) based on spectroscopic data through ir, uv, 1h-nmr, 13c-nmr along with two dimensional (2d) techniques hmbc, hmqc, and cosy. both compounds 1 and 2 were studied in tail suspe ...201728790915
improvement in fruit quality by overexpressing mir399a in woodland strawberry.fruit quality is an important trait in strawberry and is determined by many factors. the soluble solid content in strawberry fruits is positively related to the phosphorus content. microrna399 (mir399) is involved in the regulation of phosphate (pi) homeostasis. however, the effect of mir399 on strawberry quality remains unknown. in this study, mir399a-overexpressing transgenic woodland strawberries (fragaria vesca) were obtained via an agrobacterium-mediated transformation. the phosphorus (p) c ...201728783952
evaluation of a mdmyb10/gfp43 fusion gene for its suitability to act as reporter gene in promoter studies in fragaria vesca l. 'rügen'.a malus domestica mdmyb10 transcription factor gene was previously used as visible marker for successful plant transformation. we combined the mdmyb10 transcription factor gene with a gfp gene to test its viability as a non-destructive, visual, double reporter system for functional promoter studies in transgenic strawberry plants. the gfp gene was fused to mdmyb10 to provide evidence for promoter activity in red colored cells of transformed plant tissue and to exclude artefacts resulting from st ...201728781398
an efp browser for visualizing strawberry fruit and flower transcriptomes.wild strawberry fragaria vesca is emerging as an important model system for the cultivated strawberry due to its diploid genome and availability of extensive transcriptome data and a range of molecular genetic tools. being able to better utilize these tools, especially the transcriptome data, will greatly facilitate research progress in strawberry and other rosaceae fruit crops. the electronic fluorescent pictograph (efp) software is a useful and popular tool to display transcriptome data visual ...201728674614
genome-wide analysis suggests the relaxed purifying selection affect the evolution of wox genes in pyrus bretschneideri, prunus persica, prunus mume, and fragaria vesca.wuschel-related homeobox (wox) family is one of the largest group of transcription factors (tfs) specifically found in plant kingdom. wox tfs play an important role in plant development processes and evolutionary novelties. although the roles of woxs in arabidopsis and rice have been well-studied, however, little are known about the relationships among the main clades in the molecular evolution of these genes in rosaceae. here, we carried out a genome-wide analysis and identified 14, 10, 10, and ...201728663757
genetic dissection of adventitious shoot regeneration in roses by employing genome-wide association studies.we analysed the capacity to regenerate adventitious shoots in 96 rose genotypes and found 88 snp markers associated with qtls, some of which are derived from candidate genes for shoot regeneration. in an association panel of 96 rose genotypes previously analysed for petal colour, we conducted a genome-wide association study on the capacity of leaf petioles for direct shoot regeneration. shoot regeneration rate and shoot ratio (number of shoots/total number of explants) were used as phenotypic de ...201728647832
genome-wide analysis suggests high level of microsynteny and purifying selection affect the evolution of ein3/eil family in rosaceae.the ethylene-insensitive3/ethylene-insensitive3-like (ein3/eil) proteins are a type of nuclear-localized protein with dna-binding activity in plants. although the ein3/eil gene family has been studied in several plant species, little is known about comprehensive study of the ein3/eil gene family in rosaceae. in this study, ten, five, four, and five ein3/eil genes were identified in the genomes of pear (pyrus bretschneideri), mei (prunus mume), peach (prunus persica) and strawberry (fragaria vesc ...201728584725
additive qtls on three chromosomes control flowering time in woodland strawberry (fragaria vesca l.).flowering time is an important trait that affects survival, reproduction and yield in both wild and cultivated plants. therefore, many studies have focused on the identification of flowering time quantitative trait locus (qtls) in different crops, and molecular control of this trait has been extensively investigated in model species. here we report the mapping of qtls for flowering time and vegetative traits in a large woodland strawberry mapping population that was phenotyped both under field c ...201728580150
direct and pollinator-mediated effects of herbivory on strawberry and the potential for improved resistance.the global decline in pollinators has partly been blamed on pesticides, leading some to propose pesticide-free farming as an option to improve pollination. however, herbivores are likely to be more prevalent in pesticide-free environments, requiring knowledge of their effects on pollinators, and alternative crop protection strategies to mitigate any potential pollination reduction. strawberry leaf beetles (slb) galerucella spp. are important strawberry pests in northern europe and russia. given ...028572811
bioinformatics identification and transcript profile analysis of the mitogen-activated protein kinase gene family in the diploid woodland strawberry fragaria vesca.mitogen-activated protein kinases (mapks) play essential roles in mediating biotic and abiotic stress responses in plants. however, the mapk gene family in strawberry has not been systematically characterized. here, we performed a genome-wide survey and identified 12 mapk genes in the fragaria vesca genome. protein domain analysis indicated that all fvmapks have typical protein kinase domains. sequence alignments and phylogenetic analysis classified the fvmapk genes into four different groups. c ...201728562633
enhancement of nutritional and bioactive compounds by in vitro culture of wild fragaria vesca l. vegetative parts.in vitro culture emerges as a sustainable way to produce bioactives for further applicability in the food industry. herein, vegetative parts of fragaria vesca l. (wild strawberry) obtained by in vitro culture were analyzed regarding nutritional and phytochemical compounds, as well as antioxidant activity. these samples proved to have higher content of protein, polyunsaturated fatty acids, soluble sugars, organic acids (including ascorbic acid) and tocopherols (mainly α-tocopherol) than wild grow ...028554629
genome-wide identification of bzip family genes involved in drought and heat stresses in strawberry (fragaria vesca).basic leucine zipper (bzip) genes are known to play a crucial role in response to various processes in plant as well as abiotic or biotic stress challenges. we have performed an identification and characterization of 50 bzip genes across the woodland strawberry (fragaria vesca) genome, which were divided into 10 clades according to the phylogenetic relationship of the strawberry bzip proteins with those in arabidopsis and rice. five categories of intron patterns were observed within basic and hi ...028487861
a comparative study on the radioprotective potential of the polyphenolic glycoconjugates from medicinal plants of rosaceae and asteraceae families versus their aglycones.radioprotective potential of the polyphenolic glycoconjugates, isolated from flowers of sanguisorba officinalis l. (so) and erigeron canadensis l. (ec), and from leaves of fragaria vesca l. (fv) and rubus plicatus whe. et n. e. (rp) as well as their aglycones (soa, eca, fva and rpa, respectively), against γ-radiation-induced lipid peroxidation in human plasma and dna damage in lymphocytes, were investigated in vitro. these properties were assessed by measuring the concentration of thiobarbituric ...201728475935
bioinformatic and expression analyses on carotenoid dioxygenase genes in fruit development and abiotic stress responses in fragaria vesca.carotenoid dioxygenases, including 9-cis-epoxycarotenoid dioxygenases (nceds) and carotenoid cleavage dioxygenases (ccds), can selectively cleave carotenoids into various apocarotenoid products that play important roles in fleshy fruit development and abiotic stress response. in this study, we identified 12 carotenoid dioxygenase genes in diploid strawberry fragaria vesca, and explored their evolution with orthologous genes from nine other species. phylogenetic analyses suggested that the nced a ...201728444444
transcriptomics, targeted metabolomics and gene expression of blackberry leaves and fruits indicate flavonoid metabolic flux from leaf to red fruit.blackberries (rubus spp.) are among the high added value food products relevant for human health due to the increasing evidence of the beneficial effects of polyphenols, which are very abundant in these fruits. interestingly, these compounds also play a role on plant physiology, being especially relevant their role in plant defense against biotic and abiotic stress. hence, we hypothesize that since blackberry fruits have high amounts of flavonols and anthocyanins, leaves would also have high amo ...028428793
early metabolic and transcriptional variations in fruit of natural white-fruited fragaria vesca genotypes.strawberry fruits (fragaria vesca) are valued for their sweet fruity flavor, juicy texture, and characteristic red color caused by anthocyanin pigments. to gain a deeper insight into the regulation of anthocyanin biosynthesis, we performed comparative metabolite profiling and transcriptome analyses of one red-fruited and two natural white-fruited strawberry varieties in two tissues and three ripening stages. developing fruit of the three genotypes showed a distinctive pattern of polyphenol accum ...201728327625
leaf dynamics and profitability in wild strawberries.leaf dynamics and carbon gain were evaluated for two species of wild strawberry, fragaria virginiana and f. vesca. five populations on sites representing a gradient of successional regrowth near ithaca, n.y., u.s.a., were studied for two or three years each. a computer-based model of plant growth and co2 exchange combined field studies of leaf biomass dynamics with previously-determined gas exchange rates to estimate carbon balances of leaves and whole plants in different environments.leaves wer ...198628311374
differential costs of sexual and vegetative reproduction in wild strawberry populations.the co2 costs of producing sexual and vegetative reproductive propagules were calculated for two species of wild strawberry, fragaria virginiana and f. vesca. five populations on sites representing a gradient of successional regrowth near ithaca, new york, usa, were studied for two or three years each. field studies of phenology, biomass, demography, and environment and laboratory studies of co2 exchange were integrated using a computerbased model of co2 dynamics to estimate costs of propagules. ...198528310869
effects of light and temperature on leaf anatomy and photosynthesis in fragaria vesca.fragaria vesca, the woodland strawberry, was grown under a series of controlled environments including variations in light intensity, average temperatures, and temperature amplitude around a constant mean. observations on co2 exchange capacities, leaf anatomy, and cell ultrastructure were made for each treatment to determine relationships between these variables. with increasing light intensity, leaf thickness, leaf density, and mesophyll cell surface area and volume per leaf surface area increa ...197728309501
effect of arbuscular mycorrhiza on inter- and intraspecific competition of two grassland species.we were interested in the role of arbuscular mycorrhiza (am) in the competition between plants of different sizes. a pot experiment of factorial design was established, in which am root colonization and competition were used as treatments. five-week-old prunella vulgaris seedlings were chosen as target plants (i.e. plants whose response to competition was studied) and the following (13 replicates of each) were used as neighbours: (1) a large, 10-week-old p. vulgaris, (2) two p. vulgaris seedling ...199628307736
endophytic fungal diversity of fragaria vesca, a crop wild relative of strawberry, along environmental gradients within a small geographical area.fungal endophytes are highly diverse ubiquitous asymptomatic microorganisms, some of which appear to be symbiotic. depending on abiotic conditions and genotype of the plant, the diversity of endophytes may confer fitness benefits to plant communities.201728168102
partial preferential chromosome pairing is genotype dependent in tetraploid rose.it has long been recognised that polyploid species do not always neatly fall into the categories of auto- or allopolyploid, leading to the term 'segmental allopolyploid' to describe everything in between. the meiotic behaviour of such intermediate species is not fully understood, nor is there consensus as to how to model their inheritance patterns. in this study we used a tetraploid cut rose (rosa hybrida) population, genotyped using the 68k wagrhsnp array, to construct an ultra-high-density lin ...201728142191
identification and transcript analysis of the tcp transcription factors in the diploid woodland strawberry fragaria vesca.plant-specific teosinte branched 1, cycloidea, and proliferating cell factors (tcp) transcription factors play versatile functions in multiple processes of plant growth and development. however, no systematic study has been performed in strawberry. in this study, 19 fvtcp genes were identified in the diploid woodland strawberry (fragaria vesca) accession heilongjiang-3. phylogenetic analysis suggested that the fvtcp genes were classified into two main classes, with the second class further divid ...201628066489
global identification of alternative splicing via comparative analysis of smrt- and illumina-based rna-seq in strawberry.alternative splicing (as) is a key post-transcriptional regulatory mechanism, yet little information is known about its roles in fruit crops. here, as was globally analyzed in the wild strawberry fragaria vesca genome with rna-seq data derived from different stages of fruit development. the as landscape was characterized and compared between the single-molecule, real-time (smrt) and illumina rna-seq platform. while smrt has a lower sequencing depth, it identifies more genes undergoing as (57.67% ...201727997733
characterization and functional analysis of calmodulin and calmodulin-like genes in fragaria vesca.calcium is a universal messenger that is involved in the modulation of diverse developmental and adaptive processes in response to various stimuli. calmodulin (cam) and calmodulin-like (cml) proteins are major calcium sensors in all eukaryotes, and they have been extensively investigated for many years in plants and animals. however, little is known about cams and cmls in woodland strawberry (fragaria vesca). in this study, we performed a genome-wide analysis of the strawberry genome and identif ...201627990153
pathway analysis and omics data visualization using pathway genome databases: fragariacyc, a case study.the species-specific plant pathway genome databases (pgdbs) based on the biocyc platform provide a conceptual model of the cellular metabolic network of an organism. such frameworks allow analysis of the genome-scale expression data to understand changes in the overall metabolisms of an organism (or organs, tissues, and cells) in response to various extrinsic (e.g. developmental and differentiation) and/or extrinsic signals (e.g. pathogens and abiotic stresses) from the surrounding environment. ...201727987175
stable epigenetic variants selected from an induced hypomethylated fragaria vesca population.epigenetic inheritance was transmitted through selection over five generations of extreme early, but not late flowering time phenotypic lines in fragaria vesca. epigenetic variation was initially artificially induced using the dna demethylation reagent 5-azacytidine (5-azac). it is the first report to explore epigenetic variant selection and phenotypic trait inheritance in strawberry. transmission frequency of these traits was determined across generations. the early flowering (ef4) and late sto ...201627965682
high-density snp-based genetic maps for the parents of an outcrossed and a selfed tetraploid garden rose cross, inferred from admixed progeny using the 68k rose snp array.dense genetic maps create a base for qtl analysis of important traits and future implementation of marker-assisted breeding. in tetraploid rose, the existing linkage maps include <300 markers to cover 28 linkage groups (4 homologous sets of 7 chromosomes). here we used the 68k wagrhsnp axiom single-nucleotide polymorphism (snp) array for rose, in combination with snp dosage calling at the tetraploid level, to genotype offspring from the garden rose cultivar 'red new dawn'. the offspring proved t ...201627818777
quantitative trait variation is revealed in a novel hypomethylated population of woodland strawberry (fragaria vesca).phenotypic variation is determined by a combination of genotype, environment and their interactions. the realization that allelic diversity can be both genetic and epigenetic allows the environmental component to be further separated. partitioning phenotypic variation observed among inbred lines with an altered epigenome can allow the epigenetic component controlling quantitative traits to be estimated. to assess the contribution of epialleles on phenotypic variation and determine the fidelity w ...201627809774
wild fragaria vesca l. fruits: a rich source of bioactive phytochemicals.wild fragaria vesca l. fruits were studied regarding nutritional and phytochemical compounds, and also antioxidant, antibacterial and biofilm formation inhibition activities. the fruits are good sources of carbohydrates (e.g., sucrose), soluble dietary fiber and polyunsaturated fatty acids, mainly linoleic and linolenic acids, as well as other components such as citric and succinic acids, and vitamins b9 and e (mainly γ-tocopherol). significant amounts of soluble sugars, citric acid and some amo ...201627775146
polyphenolic glycoconjugates from medical plants of rosaceae/asteraceae family protect human lymphocytes against γ-radiation-induced damage.radioprotective effects of the water-soluble polyphenolic glycoconjugates, isolated from flowers of sanguisorba officinalis l.(so) and erigeron canadensis l.(ec), and from leaves of fragaria vesca l. (fv) and rubus plicatus whe. et n. e. (rp), against γ-radiation-induced toxicity in human peripheral blood lymphocytes were investigated. cell treatment with glycoconjugates (1, 5 and 25μg/ml) prior exposure to 10/15gy radiation resulted in concentration-dependent reduction of dna damage including o ...201727769934
complete nucleotide sequence of strawberry vein banding virus chinese isolate and infectivity of its full-length dna clone.strawberry vein banding virus (svbv) is a double-stranded dna plant virus, which has been found in north america, australia, brazil, japan, europe and several provinces of china. infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins. it is one of the most economically important diseases in asiatic, european and north american strawberry-growing areas.201627716385
genome-wide identification and expression analysis of mrlk family genes associated with strawberry (fragaria vesca) fruit ripening and abiotic stress responses.malectin-like domain-containing receptor-like kinases (mrlk) constitute a large and divergent family of proteins in plants; however, little is known about the role of mrlks in fruit growth and development. in this study, we characterized mrlk family genes in diploid strawberry, fragaria vesca. based on an analysis of malectin-like domain and a search in the strawberry genome and ncbi database, we identified 62 fvmrlks in the strawberry genome, and classified these genes into six subfamilies with ...201727685863
identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected lactobacillus strains.this study aimed to identify lactic acid bacteria (lab) in byproducts of fruit (malpighia glabra l., mangifera indica l., annona muricata l., and fragaria vesca l.) pulp processing. fifty strains of lab were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequence (16s rrna) analysis. species belonging to lactobacillus genus were the predominant lab in all fruit pulp processing byproducts. the average congruency betwe ...201627625647
calcium/calmodulin alleviates substrate inhibition in a strawberry udp-glucosyltransferase involved in fruit anthocyanin biosynthesis.udp-glucosyltransferase (ugt) is a key enzyme for anthocyanin biosynthesis, which by catalyzing glycosylation of anthocyanidins increases their solubility and accumulation in plants. previously we showed that pre-harvest spray of cacl2 enhanced anthocyanin accumulation in strawberry fruit by stimulating the expression of anthocyanin structural genes including a fruit specific fvugt1.201627609111
genomic identification, phylogeny, and expression analysis of mlo genes involved in susceptibility to powdery mildew in fragaria vesca.the mlo (powdery mildew locus o) gene family is important in resistance to powdery mildew (pm). in this study, all of the members of the mlo family were identified and analyzed in the strawberry (fragaria vesca) genome. the strawberry contains at least 20 members of the mlo family, and the protein sequence contained between 171 and 1485 amino acids, with 0-34 introns. chromosomal localization showed that the mlos were unevenly distributed on each of the chromosomes, except for chromosome 4. the ...201627525914
reprogramming of strawberry (fragaria vesca) root transcriptome in response to phytophthora cactorum.crown rot (phytophthora cactorum) causes significant economic losses in strawberry production. the best control strategy would be to use resistant cultivars, but polygenically inherited resistance makes the breeding of the garden strawberry (fragaria × ananassa) challenging. the diploid wild strawberry fragaria vesca hawaii 4 genotype was shown previously to have resistance against crown rot. to explore the resistance mechanisms, we inoculated the roots of hawaii 4 with p. cactorum in a novel in ...201627518577
expression of resistance gene analogs in woodland strawberry (fragaria vesca) during infection with phytophthora cactorum.important losses in strawberry production are often caused by the oomycete phytophthora cactorum, the causal agent of crown rot. however, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. to begin to rectify this situation, a pcr-based approach (nbs profiling) was used to isolate strawberry resistance gene analogs (rgas) with altered expression in response to p. cactorum during a time course (2, 4, 6, 24, 48, 96 and 192 h pos ...201627447867
transcriptome analysis of woodland strawberry (fragaria vesca) response to the infection by strawberry vein banding virus (svbv).woodland strawberry (fragaria vesca) infected with strawberry vein banding virus (svbv) exhibits chlorotic symptoms along the leaf veins. however, little is known about the molecular mechanism of strawberry disease caused by svbv.201627411713
genome-wide identification and characterization of r2r3myb family in rosaceae.transcription factors r2r3myb family have been associated with the control of secondary metabolites, development of structures, cold tolerance and response to biotic and abiotic stress, among others. in recent years, genomes of rosaceae botanical family are available. although this information has been used to study the karyotype evolution of these species from an ancestral genome, there are no studies that treat the evolution and diversity of gene families present in these species or in the bot ...201627408811
genome-scale dna variant analysis and functional validation of a snp underlying yellow fruit color in wild strawberry.fragaria vesca is a species of diploid strawberry being developed as a model for the octoploid garden strawberry. this work sequenced and compared the genomes of three f. vesca accessions: 'hawaii 4', 'rügen', and 'yellow wonder'. genome-scale analyses of shared and distinct snps among these three accessions have revealed that 'rügen' and 'yellow wonder' are more similar to each other than they are to 'hawaii 4'. though all three accessions are inbred seven generations, each accession still poss ...201627377763
detection of strawberry necrotic shock virus using conventional and taqman(®) quantitative rt-pcr.graft-indexing of an advanced selection from the university of florida strawberry breeding program produced virus-like symptoms on fragaria vesca. however; rt-pcr testing of the material did not detect the presence of any of 16 strawberry virus species or members of virus groups for which strawberries are routinely indexed. large scale sequencing of the material revealed the presence of an isolate of strawberry necrotic shock virus. the nucleotide sequence of this isolate from florida shows a si ...201627283883
the genome of black raspberry (rubus occidentalis).black raspberry (rubus occidentalis) is an important specialty fruit crop in the us pacific northwest that can hybridize with the globally commercialized red raspberry (r. idaeus). here we report a 243 mb draft genome of black raspberry that will serve as a useful reference for the rosaceae and rubus fruit crops (raspberry, blackberry, and their hybrids). the black raspberry genome is largely collinear to the diploid woodland strawberry (fragaria vesca) with a conserved karyotype and few notable ...201627228578
genome-wide identification and comparative analysis of the cation proton antiporters family in pear and four other rosaceae species.the monovalent cation proton antiporters (cpas) play essential roles in plant nutrition, development, and signal transduction by regulating ion and ph homeostasis of the cell. the cpas of plants include the na(+)/h(+) exchanger, k(+) efflux antiporter, and cation/h(+) exchanger families. however, currently, little is known about the cpa genes in rosaceae species. in this study, 220 cpa genes were identified from five rosaceae species (pyrus bretschneideri, malus domestica, prunus persica, fragar ...201627193473
characterization and comparison of the cpk gene family in the apple (malus × domestica) and other rosaceae species and its response to alternaria alternata infection.as one of the ca2+ sensors, calcium-dependent protein kinase (cpk) plays vital roles in immune and stress signaling, growth and development, and hormone responses, etc. recently, the whole genome of apple (malus × domestica), pear (pyrus communis), peach (prunus persica), plum (prunus mume) and strawberry (fragaria vesca) in rosaceae family has been fully sequenced. however, little is known about the cpk gene family in these rosaceae species. in this study, 123 cpk genes were identified from fiv ...201627186637
genome-wide analysis of the expression of wrky family genes in different developmental stages of wild strawberry (fragaria vesca) fruit.wrky proteins play important regulatory roles in plant developmental processes such as senescence, trichome initiation and embryo morphogenesis. in strawberry, only fawrky1 (fragaria × ananassa) has been characterized, leaving numerous wrky genes to be identified and their function characterized. the publication of the draft genome sequence of the strawberry genome allowed us to conduct a genome-wide search for wrky proteins in fragaria vesca, and to compare the identified proteins with their ho ...201627138272
the wrky transcription factors in the diploid woodland strawberry fragaria vesca: identification and expression analysis under biotic and abiotic stresses.wrky proteins comprise a large family of transcription factors that play important roles in response to biotic and abiotic stresses and in plant growth and development. to date, little is known about the wrky gene family in strawberry. in this study, we identified 62 wrky genes (fvwrkys) in the wild diploid woodland strawberry (fragaria vesca, 2n = 2x = 14) accession heilongjiang-3. according to the phylogenetic analysis and structural features, these identified strawberry fvwrky genes were clas ...201627105420
homomorphic zw chromosomes in a wild strawberry show distinctive recombination heterogeneity but a small sex-determining region.recombination in ancient, heteromorphic sex chromosomes is typically suppressed at the sex-determining region (sdr) and proportionally elevated in the pseudoautosomal region (par). however, little is known about recombination dynamics of young, homomorphic plant sex chromosomes. we examine male and female function in crosses and unrelated samples of the dioecious octoploid strawberry fragaria chiloensis in order to map the small and recently evolved sdr controlling both traits and to examine rec ...201627102236
flavobacterium tyrosinilyticum sp. nov., isolated from the rhizosphere of wild strawberry.a gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterial strain, designated as thg-dn8.8t, was isolated from the rhizosphere of a wild strawberry plant, located on gyeryong mountain, daejeon, republic of korea. according to the 16s rrna gene sequence comparisons, the isolate was identified as a member of the genus flavobacterium and is most closely related to flavobacterium banpakuense 15f3t (97.7 % sequence similarity), flavobacterium ginsenosidimutans thg-01t (97.3 %), f ...201627098975
identification and characterization of histone lysine methylation modifiers in fragaria vesca.the diploid woodland strawberry (fragaria vesca) is an important model for fruit crops because of several unique characteristics including the small genome size, an ethylene-independent fruit ripening process, and fruit flesh derived from receptacle tissues rather than the ovary wall which is more typical of fruiting plants. histone methylation is an important factor in gene regulation in higher plants but little is known about its roles in fruit development. we have identified 45 set methyltran ...201627049067
fragariacyc: a metabolic pathway database for woodland strawberry fragaria vesca.fragariacyc is a strawberry-specific cellular metabolic network based on the annotated genome sequence of fragaria vesca l. ssp. vesca, accession hawaii 4. it was built on the pathway-tools platform using metacyc as the reference. the experimental evidences from published literature were used for supporting/editing existing entities and for the addition of new pathways, enzymes, reactions, compounds, and small molecules in the database. to date, fragariacyc comprises 66 super-pathways, 488 uniqu ...201626973684
characterization of dna methyltransferase and demethylase genes in fragaria vesca.dna methylation is an epigenetic modification essential for gene regulations in plants, but understanding on how it is involved in fruit development, especially in non-climacteric fleshy fruit, is limited. the diploid woodland strawberry (fragaria vesca) is an important model for non-climacteric fruit crops. in this study, we identified dna methyltransferase genes and demethylase genes in fragaria vesca and other angiosperm species. in accordance with previous studies, our phylogenetic analyses ...201626956009
analysis of block of cell proliferation 1 (bop1) activity in strawberry and arabidopsis.block of cell proliferation (bop) proteins are conserved among eukaryotes, and studies in mammals and yeast have described their role in ribosome biogenesis and cell cycle regulation. a bop1 orthologue was identified in plants, and loss-of-function analyses in tobacco cells confirmed similar activities. this report characterizes a role for bop1 activity in planta. two transgenic plant species were used: the diploid strawberry (fragaria vesca) and arabidopsis thaliana. fvbop1 silencing showed cha ...201626940494
polyphenolic-polysaccharide conjugates from plants of rosaceae/asteraceae family as potential radioprotectors.polyphenolic-polysaccharide macromolecular, water-soluble glycoconjugates, isolated from the selected medicinal plants of rosaceae/asteraceae family: from leaves of fragaria vesca l., rubus plicatus whe. et n. e., and from flowering parts of sanguisorba officinalis l., and erigeron canadensis l., were investigated for their ability to protect proteins and lipids of human plasma against γ-radiation-induced oxidative damage. treatment of plasma with plant conjugates (6, 30, 150 μg/ml) prior exposu ...201626848834
divergence of the bzip gene family in strawberry, peach, and apple suggests multiple modes of gene evolution after duplication.the basic leucine zipper (bzip) transcription factors are the most diverse members of dimerizing transcription factors. in the present study, 50, 116, and 47 bzip genes were identified in malus domestica (apple), prunus persica (peach), and fragaria vesca (strawberry), respectively. species-specific duplication was the main contributor to the large number of bzips observed in apple. after wgd in apple genome, orthologous bzip genes corresponding to strawberry on duplicated regions in apple genom ...201526770968
diversity arrays technology (dart) marker platforms for diversity analysis and linkage mapping in a complex crop, the octoploid cultivated strawberry (fragaria × ananassa).cultivated strawberry (fragaria × ananassa) is a genetically complex allo-octoploid crop with 28 pairs of chromosomes (2n = 8x = 56) for which a genome sequence is not yet available. the diploid fragaria vesca is considered the donor species of one of the octoploid sub-genomes and its available genome sequence can be used as a reference for genomic studies. a wide number of strawberry cultivars are stored in ex situ germplasm collections world-wide but a number of previous studies have addressed ...201526675207
transcript quantification by rna-seq reveals differentially expressed genes in the red and yellow fruits of fragaria vesca.fragaria vesca (2n = 2x = 14), the woodland strawberry, is a perennial herbaceous plant with a small sequenced genome (240 mb). it is commonly used as a genetic model plant for the fragaria genus and the rosaceae family. fruit skin color is one of the most important traits for both the commercial and esthetic value of strawberry. anthocyanins are the most prominent pigments in strawberry that bring red, pink, white, and yellow hues to the fruits in which they accumulate. in this study, we conduc ...201526636322
the accumulation of elements in plants growing spontaneously on small heaps left by the historical zn-pb ore mining.the study evaluated the levels of nine metals, namely ca, cd, fe, k, mg, mn, pb, tl, and zn, in soils and tissues of ten plant species growing spontaneously on heaps left by historical mining for zn-pb ores. the concentrations of cd, pb, tl, and zn in heap soils were much higher than in control soils. plants growing on heaps accumulated excessive amounts of these elements in tissues, on average 1.3-52 mg cd kg(-1), 9.4-254 mg pb kg(-1), 0.06-23 mg tl kg(-1) and 134-1479 mg zn kg(-1) in compariso ...201626635220
a highly specific microrna-mediated mechanism silences ltr retrotransposons of strawberry.small rnas are involved in a plethora of functions in plant genomes. in general, transcriptional gene silencing is mediated by 24-nucleotide sirnas and is required for maintaining transposable elements in a silenced state. however, micrornas are not commonly associated with transposon silencing. in this study, we performed small rna transcriptome and degradome analyses of the rosaceae model plant fragaria vesca (the woodland strawberry) at the genome-wide level, and identified mirna families and ...201626611654
genetic dissection of the (poly)phenol profile of diploid strawberry (fragaria vesca) fruits using a nil collection.over the last few years, diploid strawberry (fragaria vesca) has been recognized as a model species for applied research of cultivated strawberry (fragaria × ananassa) that is one of the most economically important crops. berries, particularly strawberries, are known for their high antioxidant capacity due to a high concentration of (poly) phenolic compounds. studies have already characterized the phenolic composition of fruits from sets of cultivated strawberries but the quantification of pheno ...201626566833
the content of secondary metabolites and antioxidant activity of wild strawberry fruit (fragaria vesca l.).chemical analyses carried out in 2011-2013 aimed at evaluating the contents of flavonoids, free phenolic acids, tannins, anthocyanins, and antioxidant activity (%) by means of dpph radical neutralization ability in fresh and air-dried fruits of three wild strawberry cultivars. examinations revealed differences in contents of biologically active substances determined in raw versus dried material depending on the cultivar. mean concentrations of flavonoids and tannins were highest in raw fruits of ...201526539306
the effect of fragaria vesca extract on smear layer removal: a scanning electron microscopic evaluation.successful endodontic treatment depends on elimination of the microorganisms through chemomechanical debridement. the aim of this in vitro study was to evaluate the effectiveness of fragaria vesca (wild strawberry) extract (fve) on the removal of smear layer (sl).201526526069
comparative analysis of polygalacturonase in the fruit of strawberry cultivars.the role of polygalacturonase (pg) in the development, ripening, and softening of fruit from two strawberry cultivars with different flesh firmness and softening characteristics was compared. changes in pg activity and gene expression during development, ripening and softening were measured. the pg genes from each cultivar were cloned and analyzed, and were classified with other pg genes using phylogenetic analysis. in toyonoka fruit, pg activity increased gradually, reaching a peak during the p ...201526505428
molecular genetics and genomics of the rosoideae: state of the art and future perspectives.the rosoideae is a subfamily of the rosaceae that contains a number of species of economic importance, including the soft fruit species strawberry (fragaria ×ananassa), red (rubus idaeus) and black (rubus occidentalis) raspberries, blackberries (rubus spp.) and one of the most economically important cut flower genera, the roses (rosa spp.). molecular genetics and genomics resources for the rosoideae have developed rapidly over the past two decades, beginning with the development and application ...201426504527
live substrate positively affects root growth and stolon direction in the woodland strawberry, fragaria vesca.studies of clonal plant foraging generally focus on growth responses to patch quality once rooted. here we explore the possibility of true plant foraging; the ability to detect and respond to patch resource status prior to rooting. two greenhouse experiments were conducted to investigate the morphological changes that occur when individual daughter ramets of fragaria vesca (woodland strawberry) were exposed to air above live (non-sterilized) or dead (sterilized) substrates. contact between daugh ...201526483826
multilocus sex determination revealed in two populations of gynodioecious wild strawberry, fragaria vesca subsp. bracteata.gynodioecy, the coexistence of females and hermaphrodites, occurs in 20% of angiosperm families and often enables transitions between hermaphroditism and dioecy. clarifying mechanisms of sex determination in gynodioecious species can thus illuminate sexual system evolution. genetic determination of gynodioecy, however, can be complex and is not fully characterized in any wild species. we used targeted sequence capture to genetically map a novel nuclear contributor to male sterility in a self-pol ...201526483011
global identification and analysis of long non-coding rnas in diploid strawberry fragaria vesca during flower and fruit development.long non-coding rnas (lncrnas) are a new class of regulatory molecules with roles in diverse biological processes. while much effort has been invested in the analysis of lncrnas from established plant models arabidopsis, maize, and rice, almost nothing is known about lncrnas from fruit crops, including those in the rosaceae family.201526481460
functional characterization and substrate promiscuity of ugt71 glycosyltransferases from strawberry (fragaria × ananassa).glycosylation determines the complexity and diversity of plant natural products. to characterize fruit ripening-related udp-dependent glycosyltransferases (ugts) functionally in strawberry, we mined the publicly available fragaria vesca genome sequence and found 199 putative ugt genes. candidate ugts whose expression levels were strongly up-regulated during fruit ripening were cloned from f.×ananassa and six were successfully expressed in escherichia coli and biochemically characterized. ugt75t1 ...201526454881
identification, isolation, and expression analysis of heat shock transcription factors in the diploid woodland strawberry fragaria vesca.heat shock transcription factors (hsfs) are known to play dominant roles in plant responses to heat, as well as other abiotic or biotic stress stimuli. while the strawberry is an economically important fruit plant, little is known about the hsf family in the strawberry. to explore the functions of strawberry hsfs in abiotic and biotic stress responses, this study identified 17 hsf genes (fvhsfs) in a wild diploid woodland strawberry (fragaria vesca, 2n = 2x = 14) and isolated 14 of these genes. ...201526442049
synteny analysis in rosids with a walnut physical map reveals slow genome evolution in long-lived woody perennials.mutations often accompany dna replication. since there may be fewer cell cycles per year in the germlines of long-lived than short-lived angiosperms, the genomes of long-lived angiosperms may be diverging more slowly than those of short-lived angiosperms. here we test this hypothesis.201526383694
unfoldome variation upon plant-pathogen interactions: strawberry infection by colletotrichum acutatum.intrinsically disordered proteins (idps) are proteins that lack secondary and/or tertiary structure under physiological conditions. these proteins are very abundant in eukaryotic proteomes and play crucial roles in all molecular mechanisms underlying the response to environmental challenges. in plants, different idps involved in stress response have been identified and characterized. nevertheless, a comprehensive evaluation of protein disorder in plant proteomes under abiotic or biotic stresses ...201526245354
acylphloroglucinol biosynthesis in strawberry fruit.phenolics have health-promoting properties and are a major group of metabolites in fruit crops. through reverse genetic analysis of the functions of four ripening-related genes in the octoploid strawberry (fragaria × ananassa), we discovered four acylphloroglucinol (apg)-glucosides as native fragaria spp. fruit metabolites whose levels were differently regulated in the transgenic fruits. the biosynthesis of the apg aglycones was investigated by examination of the enzymatic properties of three re ...201526169681
novel and recently evolved microrna clusters regulate expansive f-box gene networks through phased small interfering rnas in wild diploid strawberry.the wild strawberry (fragaria vesca) has recently emerged as an excellent model for cultivated strawberry (fragaria × ananassa) as well as other rosaceae fruit crops due to its short seed-to-fruit cycle, diploidy, and sequenced genome. deep sequencing and parallel analysis of rna ends were used to identify f. vesca micrornas (mirnas) and their target genes, respectively. thirty-eight novel and 31 known mirnas were identified. many known mirnas targeted not only conserved mrna targets but also de ...201526143249
high resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of rosa.rosaceae is a family containing many economically important fruit and ornamental species. although fluorescence in situ hybridization (fish)-based physical mapping of plant genomes is a valuable tool for map-based cloning, comparative genomics and evolutionary studies, no studies using high resolution physical mapping have been performed in this family. previously we proved that physical mapping of single-copy genes as small as 1.1 kb is possible on mitotic metaphase chromosomes of rosa wichuran ...201526134672
convergent evolution at the gametophytic self-incompatibility system in malus and prunus.s-rnase-based gametophytic self-incompatibility (gsi) has evolved once before the split of the asteridae and rosidae. this conclusion is based on the phylogenetic history of the s-rnase that determines pistil specificity. in rosaceae, molecular characterizations of prunus species, and species from the tribe pyreae (i.e., malus, pyrus, sorbus) revealed different numbers of genes determining s-pollen specificity. in prunus only one pistil and pollen gene determine gsi, while in pyreae there is one ...201525993016
using rna-seq to assemble a rose transcriptome with more than 13,000 full-length expressed genes and to develop the wagrhsnp 68k axiom snp array for rose (rosa l.).in order to develop a versatile and large snp array for rose, we set out to mine ests from diverse sets of rose germplasm. for this rna-seq libraries containing about 700 million reads were generated from tetraploid cut and garden roses using illumina paired-end sequencing, and from diploid rosa multiflora using 454 sequencing. separate de novo assemblies were performed in order to identify single nucleotide polymorphisms (snps) within and between rose varieties. snps among tetraploid roses were ...201525954285
development and preliminary evaluation of a 90 k axiom® snp array for the allo-octoploid cultivated strawberry fragaria × ananassa.a high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry fragaria × ananassa. short-read sequences from one diploid and 19 octoploid accessions were aligned to the diploid fragaria vesca 'hawaii 4' reference genome to identify single nucleotide polymorphisms (snps) and indels for incorporation into a 90 k affymetrix® axiom® array. we report the development and preliminary evaluation of this array.201525886969
a near-isogenic line (nil) collection in diploid strawberry and its use in the genetic analysis of morphologic, phenotypic and nutritional characters.first near-isogenic line collection in diploid strawberry, a tool for morphologic, phenotypic and nutritional qtl analysis. diploid strawberry (fragaria vesca), with a small genome, has a high degree of synteny with the octoploid cultivated strawberry (f. × ananassa), so can be used as a simplified model for genetic analysis of the octoploid species. agronomically interesting traits are usually inherited quantitatively and they need to be studied in large segregating progenies well characterized ...201525841354
sublethal effects of imidacloprid on interactions in a tritrophic system of non-target species.imidacloprid is one of the most used insecticides worldwide, but is highly toxic to non-target arthropods. effects of sublethal imidacloprid intoxication can potentially propagate in food webs, yet little is known about the impact on non-target populations and communities. we investigated short-term sublethal toxicity of imidacloprid in a tritrophic model system of wild strawberry fragaria vesca, wood cricket nemobius sylvestris and nursery web spider pisaura mirabilis. strawberries were treated ...201525840341
the arabidopsis npr1 gene confers broad-spectrum disease resistance in strawberry.although strawberry is an economically important fruit crop worldwide, production of strawberry is limited by its susceptibility to a wide range of pathogens and the lack of major commercial cultivars with high levels of resistance to multiple pathogens. the objective of this study is to ectopically express the arabidopsis thaliana npr1 gene (atnpr1) in the diploid strawberry fragaria vesca l. and to test transgenic plants for disease resistance. atnpr1 is a key positive regulator of the long-la ...201525812515
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