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comparison of the tracheal systems of anopheles sinensis and aedes togoi larvae using synchrotron x-ray microscopic computed tomography (respiratory system of mosquito larvae using sr-μct).mosquito-borne diseases, such as malaria, dengue fever, and zika virus, are serious global health issues. vector control may be an important strategy in reducing the mortality caused by these diseases. the respiratory system of mosquito larvae in the water has to inhale atmospheric oxygen as aquatic organisms. in this study, the three-dimensional (3d) structures of the dorsal longitudinal trunks (dlts) of the tracheal systems of anopheles sinensis and aedes togoi were compared using synchrotron ...201728497875
new distribution records of mosquitoes in washington state.routine west nile virus (wnv) surveillance in 2016 by the washington state department of health resulted in 1 new state distribution record for aedes hendersoni, bringing the total number of mosquito species reported from washington to 52. we also report new county records for aedes japonicus japonicus, aedes togoi, and culex salinarius .201728388317
three-dimensional structures of the tracheal systems of anopheles sinensis and aedes togoi pupae.mosquitoes act as a vector for the transmission of disease. the world health organization has recommended strict control of mosquito larvae because of their "few, fixed, and findable" features. the respiratory system of mosquito larvae and pupae in the water has a weak point. as aquatic organisms, mosquito larvae and pupae inhale atmosphere oxygen. however, the mosquito pupae have a non-feeding stage, unlike the larvae. therefore, detailed study on the tracheal system of mosquito pupae is helpfu ...201728287649
floating of the lobes of mosquito (aedes togoi) larva for respiration.mosquito (aedes togoi) larva has to float its siphon on the water surface to breathe air. to elucidate the floating mechanism of the siphon, morphological structures, especially the flap-like lobes and spiracle of the siphon, were observed. wettability and dynamic behavior of the lobes on the water surface were also experimentally examined. the lobes formed a hollow cone shape under water and expanded on the water surface. the spiracle was located at the base of the cone. the lobes exhibited hyd ...201728216635
vertical transmission of zika virus in aedes aegypti mosquitoes.previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ...201627573623
antifilarial and antibiotic activities of methanolic extracts of melaleuca cajuputi flowers.we evaluated the activity of methanolic extracts of melaleuca cajuputi flowers against the filarial worm brugia pahangi and its bacterial endosymbiont wolbachia. anti-wolbachia activity was measured in worms and in aedes albopictus aa23 cells by pcr, electron microscopy, and other biological assays. in particular, microfilarial release, worm motility, and viability were determined. m. cajuputi flower extracts were found to significantly reduce wolbachia endosymbionts in aa23 cells, wolbachia sur ...201627417081
peculiar liquid-feeding and pathogen transmission behavior of aedes togoi and comparison with anopheles sinensis.female mosquitoes transmit various diseases as vectors during liquid-feeding. identifying the determinants of vector efficiency is a major scientific challenge in establishing strategies against these diseases. infection rate and transmission efficiency are interconnected with the mosquito-induced liquid-feeding flow as main indexes of vector efficiency. however, the relationship between liquid-feeding characteristics and pathogen remains poorly understood. the liquid-feeding behavior of aedes t ...201626839008
comparison of repellency effect of mosquito repellents for deet, citronella, and fennel oil.to confirm that korean food and drug administration (kfda) guidelines are applicable to test the efficacy of mosquito repellents, these guidelines were used to test the efficacy and complete protection times (cpts) of three representative mosquito repellents: n,n-diethyl-3-methylbenzamide (deet), citronella, and fennel oil. the repellency of citronella oil decreased over time, from 97.9% at 0 h to 71.4% at 1 h and 57.7% at 2 h, as did the repellency of fennel oil, from 88.6% at 0 h to 61.2% at 1 ...201526527362
midgut ultrastructure of fourth instar ochlerotatus togoi (diptera: culicidae).the ultrastructure of the midgut of fourth instar ochlerotatus togoi was investigated by light, scanning and transmission electron microscopy. this study was performed to provide information to help devise future control efforts aimed at the larval stages of this vector of filariasis. the fourth instar midgut was approximately 2 mm in length and consisted of three morphologically distinct cell types: epithelial, regenerative, and endocrine cells. there was a monolayer of epithelial cells on the ...201426466416
comparison of the functional features of the pump organs of anopheles sinensis and aedes togoi.mosquitoes act as vectors for severe tropical diseases. mosquito-borne diseases are affected by various factors such as environmental conditions, host body susceptibility, and mosquito feeding behavior. among these factors, feeding behavior is affected by the feeding pump system located inside the mosquito head and also depends on the species of mosquito. therefore, the 3d morphological structures of the feeding pumps of aedes togoi and anopheles sinensis were comparatively investigated using sy ...201526464043
draft genome of brugia pahangi: high similarity between b. pahangi and b. malayi.efforts to completely eradicate lymphatic filariasis from human population may be challenged by the emergence of brugia pahangi as another zoonotic lymphatic filarial nematode. in this report, a genomic study was conducted to understand this species at molecular level.201526350613
phylogeography of the coastal mosquito aedes togoi across climatic zones: testing an anthropogenic dispersal hypothesis.the coastal mosquito aedes togoi occurs more or less continuously from subarctic to subtropic zones along the coasts of the japanese islands and the east asian mainland. it occurs also in tropical southeast asia and the north american pacific coast, and the populations there are thought to have been introduced from japan by ship. to test this hypothesis, the genetic divergence among geographic populations of a. togoi was studied using one mitochondrial and three nuclear gene sequences. we detect ...201526107619
chikungunya virus-vector interactions.chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya fever, a severe, debilitating disease that often produces chronic arthralgia. since 2004, chikv has emerged in africa, indian ocean islands, asia, europe, and the americas, causing millions of human infections. central to understanding chikv emergence is knowledge of the natural ecology of transmission and vector infection dynamics. this review presents current understanding of chikv infection dynamics in mosquito v ...201425421891
susceptibility of five species members of the korean hyrcanus group to brugia malayi, and hybridization between b. malayi-susceptible and -refractory anopheles sinensis strains.five species members of the korean hyrcanus group: anopheles pullus, anopheles sinensis, anopheles kleini, anopheles belenrae, and anopheles lesteri were tested for susceptibility to brugia malayi. they were allowed to feed artificially on blood containing b. malayi microfilariae and dissected 14 days after feeding. the susceptibility rates were 60%, 65%, 90%, 100% and 100% in an. pullus, an. sinensis, an. kleini, an. belenrae, and an. lesteri, respectively. as determined by levels of susceptibi ...201424974643
detection of heparin in the salivary gland and midgut of aedes togoi.mosquitoes secrete saliva that contains biological substances, including anticoagulants that counteract a host's hemostatic response and prevent blood clotting during blood feeding. this study aimed to detect heparin, an anticoagulant in aedes togoi using an immunohistochemical detection method, in the salivary canal, salivary gland, and midgut of male and female mosquitoes. comparisons showed that female mosquitoes contained higher concentrations of heparin than male mosquitoes. on average, the ...201424850962
rapid detection and identification of wuchereria bancrofti, brugia malayi, b. pahangi, and dirofilaria immitis in mosquito vectors and blood samples by high resolution melting real-time pcr.a simple, rapid, and high-throughput method for detection and identification of wuchereria bancrofti, brugia malayi, brugia pahangi, and dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time pcr combined with high-resolution melting (hrm) analysis. amplicons of the 4 filarial species were generated from 5s rrna and spliced leader sequences by the real-time pcr and their melting temperatures were determined by the hrm method. melting of amplicons from w. bancro ...201324516268
surveillance and vector control of lymphatic filariasis in the republic of korea.until the early 2000s, lymphatic filariasis would commonly break out in the coastal areas in korea. through steady efforts combining investigation and treatment, filariasis was officially declared eradicated in 2008. this study surveyed the density of vector species of filariasis in past endemic areas, and inspected filariasis dna from collected mosquitoes for protection against the reemergence of filariasis.024159506
larvicidal activity of the active constituent isolated from tabebuia avellanedae bark and structurally related derivatives against three mosquito species.mosquito larvicidal activities of active constituent isolated from tabebuia avellanedae bark and its structurally related derivatives were examined against the fourth instar larvae of aedes aegypti, culex pipiens pallens, and ochlerotatus togoi. the insecticidal constituent of t. avellanedae bark was isolated by chromatographic techniques and identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthalenedione. on the basis of the 50% lethal concentration (lc50) values against c. pipiens pallens ...201324144401
salivary glands proteins expression of anopheles dirus a fed on plasmodium vivax- and plasmodium falciparum-infected human blood.mosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. this study aims to investigate the salivary glands proteins expression of 50 adult female an. dirus a mosquitoes, a main vector of malaria in thailand, each group with an age of 5 days which were artificial membrane fed on sugar, normal blood, blood infected with p. vivax, and blood infected with p. falciparum. then mosquito salivary gland proteins were analyze ...201323956841
development of a facile system for mass production of brugia malayi in a small-space laboratory.brugia malayi is one of the important lymphatic filarial nematodes that cause elephantiasis and disability in humans in the asian region. mass production at any stage of this nematode in both small laboratory animal hosts and mosquito vectors is still necessary in order to continue various research aspects. this study elucidated on the use of nonblood feeding or the autogenous ochlerotatus togoi (thailand strain) and male mongolian jird (meriones unguiculatus) system. this has brought about a lo ...201323820603
aedes aegypti (l.) survival after exposure to ivermectin.ivermectin has been shown in in vitro studies to have insecticidal properties against aedes aegypti adults. this study aimed to assess these properties in vivo. aedes aegypti survival was not affected by acquiring a blood meal from humans both 5 hours and 24 hours after ingestion of a typical dose of ivermectin.023691626
peritrophic matrix formation and brugia malayi microfilaria invasion of the midgut of a susceptible vector, ochlerotatus togoi (diptera: culicidae).the mosquito midgut is the first site that vector-borne pathogens contact during their multiplication, differentiation, or migration from blood meal to other tissues before transmission. after blood feeding, the mosquitoes synthesize a chitinous structure called peritrophic matrix (pm) that envelops the blood meal and separates the food bolus from the midgut epithelium. in this study, a systematic investigation of the pm formation and the interaction of brugia malayi within the midgut of a susce ...201323529338
susceptibility of eight species members in the anopheles hyrcanus group to nocturnally subperiodic brugia malayi.filariasis, caused by brugia malayi, is a public health problem in thailand. currently, at least two locations in southern thailand are reported to be active endemic areas. two and four mansonia species are primary and secondary vectors, respectively, of the nocturnally subperiodic race, whereas, coquillettidia crassipes is a vector of the diurnally subperiodic race. although several anopheles species have been incriminated extensively as natural and/or suspected vectors of b. malayi, little is ...201323289957
larval development of aedes aegypti and aedes albopictus in peri-urban brackish water and its implications for transmission of arboviral diseases.aedes aegypti (linnaeus) and aedes albopictus skuse mosquitoes transmit serious human arboviral diseases including yellow fever, dengue and chikungunya in many tropical and sub-tropical countries. females of the two species have adapted to undergo preimaginal development in natural or artificial collections of freshwater near human habitations and feed on human blood. while there is an effective vaccine against yellow fever, the control of dengue and chikungunya is mainly dependent on reducing f ...201122132243
vector mosquitoes of filariasis in japan. 022028600
brugian filariasis in japan. 022028598
lymphatic filariasis and its control in japan -the background of success-. 022028596
seasonal prevalence of mosquitoes, including vectors of brugian filariasis, in southern islands of the republic of korea.a survey of mosquitoes, including the vector status of brugia malayi filariasis and their relative larval density, was conducted from 2002 to 2005 at several southern remote islands of jeollanam-do (province), gyeongsangnam-do, and jeju-do, korea, where filariasis was previously endemic. overall, a total of 9 species belonging to 7 genera were collected. ochlerotatus togoi (formerly known as aedes togoi), anopheles (hyrcanus) group, and culex pipiens were the predominant species captured at all ...201121461270
the 6th meeting of the global alliance to eliminate lymphatic filariasis: a half-time review of lymphatic filariasis elimination and its integration with the control of other neglected tropical diseases.abstract: the 6th meeting of the global alliance to eliminate lymphatic filariasis (gaelf6) was held 1-3 june, 2010 in seoul, korea, with 150 participants from 38 countries. the year 2010 marks the midpoint between the first gaelf meeting, in 2000, and the world health organization (who) 2020 goal of global elimination of lymphatic filariasis (lf) as a public health problem. the theme of the meeting, "half-time in lf elimination: teaming up with neglected tropical diseases (ntds)," reflected sig ...201020961435
larvicidal activity of asarum heterotropoides root constituents against insecticide-susceptible and -resistant culex pipiens pallens and aedes aegypti and ochlerotatus togoi.we investigated the toxicity of (-)-asarinin, α-asarone, methyleugenol, pellitorine, and pentadecane identified in asarum heterotropoides root to third instar larvae from insecticide-susceptible culex pipiens pallens (ks-cp strain), aedes aegypti, and ochlerotatus togoi as well as field-collected c. p. pallens (dj-cp colony), identified by polymerase chain reaction. results were compared with those of two conventional mosquito larvicides: fenthion and temephos. pellitorine (lc50, 2.08, 2.33, and ...201020806890
molecular phylogenetics of aedes japonicus, a disease vector that recently invaded western europe, north america, and the hawaiian islands.we used two mitochondrial loci (nicotinamide adenine dinucleotide dehydrogenase subunit 4 and cytochrome oxidase ii) and a nuclear locus (28s-d2 spacer) for a total of 1337 bp to evaluate the relationships among the four subspecies of aedes (finlaya) japonicus theobald. ae. j. japonicus was recently introduced into the united states and has been expanding rapidly. we also included in our analysis a morphologically very closely related species, aedes (finlaya) koreicus edwards, as well as three m ...201020695267
the epidemiology and treatment of infection due to brugia malayi.the author reviews the distribution, epidemiology, and treatment of filarial infection due to brugia malayi, with special reference to malaya. b. malayi infection in man is confined to the far east between longitudes 75 degrees e and 140 degrees e and is essentially rural. the chief vectors are mansonia spp., anopheles hyrcanus group, a. barbirostris group, and aëdes togoi. the epidemiological picture is complicated by the fact that b. malayi and other closely related species have now been found ...196220604131
mosquito transcriptome profiles and filarial worm susceptibility in armigeres subalbatus.armigeres subalbatus is a natural vector of the filarial worm brugia pahangi, but it kills brugia malayi microfilariae by melanotic encapsulation. because b. malayi and b. pahangi are morphologically and biologically similar, comparing ar. subalbatus-b. pahangi susceptibility and ar. subalbatus-b. malayi refractoriness could provide significant insight into recognition mechanisms required to mount an effective anti-filarial worm immune response in the mosquito, as well as provide considerable de ...201020421927
a case of hypersensitivity to mosquito bite associated with epstein-barr viral infection and natural killer cell lymphocytosis.hypersensitivity to mosquito bites (hmb) is a disorder characterized by a necrotic skin reaction and generalized symptoms subsequent to mosquito bites. it has been suggested that hmb is associated with chronic epstein-barr virus (ebv) infection and natural killer cell leukemia/lymphoma. we describe here a korean child who had hmb associated with chronic ebv infection and natural killer cell lymphocytosis. a 5-yr-old boy was suffered from necrotic skin lesions on the right ear lobe. type a eb vir ...201020119592
successful control of lymphatic filariasis in the republic of korea.a successful experience of lymphatic filariasis control in the republic of korea is briefly reviewed. filariasis in the republic of korea was exclusively caused by infection with brugia malayi. over the past several decades from the 1950s to 2006, many investigators exerted their efforts to detection, treatment, and follow-up of filariasis patients in endemic areas, and to control filariasis. mass, combined with selective, treatments with diethylcarbamazine to microfilaria positive persons had b ...200919967079
larvicidal activity of compounds isolated from asarum heterotropoides against culex pipiens pallens, aedes aegypti, and ochlerotatus togoi (diptera: culicidae).the toxicity of several compounds isolated from asarum heterotropoides root steam distillate to third-instar larvae of culex pipiens pallens coquillett, aedes aegypti (l.), and ochlerotatus togoi theobald was examined using a direct contact mortality bioassay. safrole was the most toxic constituent to cx. p. pallens and ae. aegypti larvae, whereas terpinolene was most toxic to oc. togoi. however, lc50 values of these three mosquito larvae to both essential oils as well as the remainder of the 26 ...200919960690
fifty years of the korean society for parasitology.in 1959, the korean society for parasitology was founded by clinical scientists, specialists of public health, and 5 core parasitologists with experience in american science and medicine. the society this year celebrates its 50th anniversary. due to public health importance at the time of foundation, medical parasitology was the main stream for next 3 decades. domestic problems of niche parasitic diseases, unlisted in 6 tropical diseases of major importance, had been studied by own efforts. to c ...200919885338
ecological study of avian malaria vectors on the island of minami-daito, japan.the seasonal prevalence and spatial distribution of mosquitoes were examined as part of an avian malaria study on the oceanic island of minami-daito island, japan. because dry ice was not available in this study, yeast-generated co2 was used to attract biting mosquitoes. adult mosquitoes were collected biweekly using battery-operated traps enhanced with yeast-generated co2 and a gravid trap from march 2006 to february 2007. the co2-baited traps were distributed in 4 different habitats: sugar can ...200919852217
overwintering of anopheles lindesayi japonicus larvae in the republic of korea.mosquito larval surveillance for environmental monitoring and pest-control purposes was conducted monthly at dredged soil-dumping areas during the construction of a new harbor in yongcheon bay, approximately 5 km se of jinhae, on the sw side of namsan (mt. nam) and across the bay from su-do (su island) in gyeongsangnam province, republic of korea (rok) from november 2007 through april 2008. during this study, mosquitoes collected as overwintering larvae were aedes togoi in brackish rock pools al ...200919432066
quantitative trait loci determining autogeny and body size in the asian tiger mosquito (aedes albopictus).the majority of mosquito species require a blood meal to stimulate vitellogenesis and subsequent oviposition (anautogeny), but some autogenous individuals complete their first ovarian cycle without a blood meal. autogeny may be facultative or obligatory. in this study, we selected for an autogenous strain in the asian tiger mosquito aedes albopictus and examined an f(1) intercross population for quantitative trait loci (qtl) determining the autogeny trait as well as wing length as a proxy for bo ...200818461085
larvicidal activity of kaempferia galanga rhizome phenylpropanoids towards three mosquito species.this study was aimed at assessing the toxicity of ethyl cinnamate and ethyl p-methoxycinnamate (emc) identified in kaempferia galangal l. (zingiberaceae) rhizome and another 12 known compounds to third-instar larvae from laboratory-reared culex pipiens pallens forskal, aedes aegypti l. and ochlerotatus togoi theobald and field-collected c. pipiens pallens (jinhae colony). results were compared with those for fenthion and temephos.200818324612
ecology of invasive mosquitoes: effects on resident species and on human health.investigations of biological invasions focus on patterns and processes that are related to introduction, establishment, spread and impacts of introduced species. this review focuses on the ecological interactions operating during invasions by the most prominent group of insect vectors of disease, mosquitoes. first, we review characteristics of non-native mosquito species that have established viable populations, and those invasive species that have spread widely and had major impacts, testing wh ...200517637849
mosquitoes do senesce: departure from the paradigm of constant mortality.although variation in mortality is considered by virtually all vector-borne disease specialists to be one of the most important determinants of an arthropod's capacity to transmit pathogens, the operational assumption often is that insect vector mortality is independent of age. acceptance of the non-senescence assumption leads to the erroneous conclusion that mosquito age is unimportant, results in misleading predictions regarding disease reductions after vector control, and represses study of o ...200717255238
mosquito larvicidal activity of active constituent derived from chamaecyparis obtusa leaves against 3 mosquito species.mosqutio larvicidal activity of chamaecyparis obtusa leaf-derived materials against the 4th-stage larvae of aedes aegypti (l.), ochlerotatus togoi (theobald), and culex pipiens pallens (coquillett) was examined in the laboratory. a crude methanol extract of c. obtusa leaves was found to be active (percent mortality rough) against the 3 species larvae; the hexane fraction of the methanol extract showed a strong larvicidal activity (100% mortality) at 100 ppm. the bioactive component in the c. obt ...200516506565
hypersensitivity to forcipomyia taiwana (biting midge): clinical analysis and identification of major for t 1, for t 2 and for t 3 allergens.forcipomyia taiwana is a tiny, blood-sucking midge that cause intense pruritus and swelling in sensitive individuals. it is distributed island-wide in rural taiwan and southern china. objective: this study aimed to study the allergic immune responses and identify f. taiwana allergens.200516266384
distribution of ochlerotatus togoi along the pacific coast of washington.before this study, the mosquito ochlerotatus (finlaya) togoi (theobald) had been reported from only 2 locations within the continental united states, both of which were documented in washington state. this study used active and passive surveillance to determine the current distribution of oc. togoi along the pacific coast of washington. results of the study show that small, but stable, populations of oc. togoi exist in the northern san juan island region of puget sound. geological formations in ...200415264615
mosquito appetite for blood is stimulated by plasmodium chabaudi infections in themselves and their vertebrate hosts.arthropod vectors of disease may encounter more than one infected host during the course of their lifetime. the consequences of super-infection to parasite development are rarely investigated, but may have substantial epidemiological and evolutionary consequences.200415151700
repellency of aromatic turmeric curcuma aromatica under laboratory and field conditions.three curcuma species, curcuma aeruginosa (pink and blue ginger), cu. aromatica (aromatic turmeric), and cu. xanthorrhiza (giant curcuma), were selected for investigation of mosquito repellent activity. in a laboratory study, a 95% ethanol extract of each plant was tested for repellent activity of aedes togoi on human volunteers. only cu. aromatica extract showed repellency against ae. togoi with ed50 and ed95 values of 0.061 and 1.55 mg/cm2, respectively. it also provided biting protection for ...200314714673
behaviour of filariae: morphological and anatomical signatures of their life style within the arthropod and vertebrate hosts.this paper attempts to pinpoint the most original morphological anatomical features of the biology of filariae per se and those which are or could be important for triggering regulatory processes in the arthropod vector and uncontrolled pathogenic processes in the vertebrate hosts. the following stages are considered: the motile egg or newly-hatched larva, the microfilaria, in the lymphatic or blood vessels of its vertebrate host; the larva, its migrations and its intrasyncitial development in t ...200314675490
emodin isolated from cassia obtusifolia (leguminosae) seed shows larvicidal activity against three mosquito species.mosquito larvicidal activity of cassia obtusifolia (leguminosae) seed-derived materials against the fourth-instar larvae of aedes aegypti, aedes togoi, and culex pipiens pallens was examined. the chloroform fraction of c. obtusifolia extract showed a strong larvicidal activity of 100% mortality at 25 mg/l. the biologically active component of c. obtusifolia seeds was characterized as emodin by spectroscopic analyses. the lc(50) values of emodin were 1.4, 1.9, and 2.2 mg/l against c. pipiens pall ...200314664519
laboratory transmission of semliki forest virus by aedes togoi theo. 196014427965
aedes (finlaya) togoi theobold, a useful laboratory vector in studies of filariasis. 196314101934
a review of the role of mosquitoes in the transmission of malayan and bancroftian filariasis in japan.malayan filariasis is found in japan only on the small island of hachijo-koshima and is transmitted there by aëdes togoi and probably by culex pipiens pallens.bancroftian filariasis is widely distributed in the three main islands, and is of particularly high endemicity in the south. of the ten mosquito species proved experimentally susceptible to wuchereria bancrofti, only aëdes togoi and culex p. pallens seem to be responsible for transmission of the disease. the former species is of importance ...196213940117
on the feeding habits of aedes togoi and some other species of mosquitoes. 195313090790
comparative studies on the biology and filarial susceptibility of selected blood-feeding and autogenous aedes togoi sub-colonies.blood-feeding and autogenous sub-colonies were selected from a laboratory, stock colony of aedes togoi, which was originally collected from koh nom sao, chanthaburi province, southeast thailand. comparative biology and filarial susceptibility between the two sub-colonies (blood-feeding: f11, f13; autogeny: f38, f40) were investigated to evaluate their viability and vectorial capacity. the results of comparison on biology revealed intraspecific differences, i.e., the average egg deposition/gravid ...200312937758
[the epidemiological studies on the filariasis in korea: i. filariasis in cheju-do(quelpart island)]a night blood survey was carried out among inhabitants aged over 1 year from the fifteen villages throughout cheju-do (quelpart island). blood films from 2,139 persons were examined and 183(8.6 %) showed microfilariae, the incidences varying according to geographical sources are from 0.8 to 19.5 per cent. all the microfilariae found in this survey were of the nocturnal periodic brugia malayi. the microfilarial density was 1.9 per cent of blood. the age and sex distributions of microfilaria rate ...196512913587
[studies on filariasis in korea: on the morphology and development of larvae of brugia malayi in aedes togoi]since senoo and lincicome (1951) first have brought up for attention to the existence of malayan filariasis in korea, several reports on the epidemiological investigations of the disease had already been made by many workers. however it is little known what kind of mosquitoes are involved as the major vectors in main endemic areas. in cheju-do, known as one of main endemic areas in korea, aedes togoi is most likely suspected as an important vector because of their abundant collections and vigoro ...196812913548
[a study on aedes togoi as vector of filariasis in che ju lsland]a study on the aedes togoi to find out important biological and ecological factors as an efficient vector of filariasis, was carried out on che-ju island for two years from 1968 though 1969. results obtained are as followings: 1)geographical distribution of the mosquito breeding habitat was found to be extensive covering circular areas of about three km distance along the shore line. the main breeding sites were rockpools and smaller holes on rocks retaining tide and/or rain water with high larv ...196912913528
the effects of common salt on aedes togoi and filarial infection.the effects of salt on the development of aedes togoi and the infecton of brugia pahangi were studied. salt concentrations over 7.2% completely got rid of pupae and adults. the salt concentrations 0% to 3.6% reduced pupal development from 86.6% to 72.8% and adult from 70.3% to 53.3%. b. pahangi infestations and infection rates were also reduced in the mosquitoes reared at salt concentrations of 1.8% and 3.6%. trends of retardation in the filarial development were observed when the salt concentra ...198212902695
cross-reactivity between sera from dogs experimentally infected with dirofilaria immitis and crude extract of toxocara canis.this study was performed to investigate whethere there is cross-reactivity between dirofilaria immitis and three intestinal nematodes of dogs. in elisa, d. immitis-infected dog sera obtained at the 4th molting stage (9-11 weeks) and microfilaremic stage (25-30 weeks) were shown to be highly reactive with crude extract of t. canis. in immunoblotting, some antigenic fractions, 44, 57, 88, 100 kda of crude extract of t. canis, were found to be positive reaction with sera of dogs infected with d. im ...200212509105
characterization of human ige and mouse igg1 responses to allergens in three mosquito species by immunoblotting and elisa.ige-mediated allergic reactions caused by mosquito bites are a common problem all over the world. this study was undertaken to determine ige levels in subjects, to elucidate human ige and mouse igg1 binding patterns and to investigate the cross-reactivity of salivary gland antigens with three mosquitoes.200111752877
field evaluation of an insect growth regulator, pyriproxyfen, against aedes togoi larvae in brackish water in south korea.the granular formulation of 0.5% pyriproxyfen was evaluated for inhibition of emergence of aedes togoi in brackish water of rock pools near a coastal area in pusan, south korea. complete adult emergence inhibition in 4th-stage larval and pupal isolations was affected from 5-40 days at 0.05 mg/l after treatment. most inhibition rates were over 80% throughout the test period at all test concentrations of pyriproxyfen except 61.0% and 67.5% of inhibition rates at 0.01 mg/l at 52 days and 62 days af ...200111469183
autogenous aedes togoi sub-colony (chanthaburi, thailand strain), an efficient laboratory vector in study of filariasis.comparative filarial susceptibility and biology between stock colony and selectively autogenous aedes. togoi sub-colony were carried out to determine the laboratorial vector-capacity and viability of autogenous sub-colony. the results of susceptibility revealed that the selectively autogenous ae. togoi sub-colony yielded higher susceptibility than the stock colony, ie dirofilaria immitis: susceptibility rates=80.00% [exp1(f8)] and 76.19% [exp2(f17)] (autogenous sub-colony), 53.33% (exp1) and 71. ...200011127320
predation efficacy of the fish muddy loach, misgurnus mizolepis, against aedes and culex mosquitoes in laboratory and small rice plots.assessments of the biological control potential of muddy loaches, misgurnus mizolepis, were conducted against aedes togoi, culex pipiens pallens, and culex inatomii larvae both in the laboratory and in small rice plots in pusan, korea, from june to september 1997. medium-sized fish consumed almost all of 500 3rd-stage larvae of cx. pipiens pallens offered daily in the laboratory. predation by the fish at release rates of 1, 2, 4, 8, and 16 fish per plot resulted in 1,004, 1,197, 1,198, 1,200, an ...200011081657
genetics of mosquito vector competence.mosquito-borne diseases are responsible for significant human morbidity and mortality throughout the world. efforts to control mosquito-borne diseases have been impeded, in part, by the development of drug-resistant parasites, insecticide-resistant mosquitoes, and environmental concerns over the application of insecticides. therefore, there is a need to develop novel disease control strategies that can complement or replace existing control methods. one such strategy is to generate pathogen-resi ...200010704476
reduction in susceptibility to brugia malayi of f2 progeny of aedes togoi treated with ethyl methanesulfonate.the susceptibility to brugia malayi infection was tested in f2 female progeny derived from male and female aedes togoi treated with ethyl methanesulfonate (ems). three-day-old males and females were treated with 0.025, 0.050, and 0.075, 0.10, 0.15, or 0.20% ems by allowing them to feed for 5 days on sugar cubes containing ems and then mated at random. percentage of susceptibility and mean number of infective larvae (l3) in f2 females were analyzed over a 2-wk period. reductions in susceptibility ...199910612608
variation in acid hydrolase enzyme titers in different developmental stages of aedes togoi.genetic mechanisms of filarial nematode susceptibility were studied in aedes togoi. acid hydrolases may play an important role in this process, including humoral or cell-mediated defenses. levels of acid phosphatase, alpha-glucosidase, beta-glucuronidase, and n-acetyl-beta-glucosaminidase were determined for 1st- and 4th-instar larvae, male and female pupae, and 1- and 7-day-old adults using fluorometric and colorimetric assays. acid phosphatase activity was highest in 1-day-old adults, moderate ...199810084130
sulphate conjugation of serotonin and n-acetylserotonin in the mosquito, aedes togoi.the sulphate conjugation of serotonin and n-acetylserotonin (nas) was studied in the mosquito, aedes togoi, using a high-pressure liquid chromatography-radioisotopic procedure. this involved the transfer of the sulphate group from 3'-phosphoadenosine-5'-phosphosulphate (pap35s) to serotonin or nas by phenolsulphotransferase (pst). nas, the acetylated product, is less polar than serotonin but it is a better substrate of pst. its removal would conceivably be facilitated by sulphate conjugation whi ...19938508189
[studies on the susceptibility of anopheles anthropophagus to experimental infection with wuchereria bancrofti].this paper reports the susceptibility of anopheles anthropophagus (xu et feng, 1975) to experimental infection with wuchereria bancrofti (cobbold, 1877), comparing with that of an. sinensis wiedemann, 1828, culex pipiens quinque fasciatus say, 1823, and aedes togoi (theobald, 1907). of 188 an. anthropophagus, 202 an. sinensis, 280 cx. pipiens quinque fasciatus and 129 ae. togoi infected by the blood of a bancroftian microfilaremia with mff density of 190 mff/20cmm, the infective rates were 35.64 ...19938174212
acetyl coa generation and n-acetylation of serotonin (5ht) in the mosquito, aedes togoi.acetyl coa synthetase (acs; ec 6.2.1.1) was studied in the mosquito, aedes togoi, by a novel assay which coupled the acetyl-coa generated to p-aminosalicylic acid (asa). the n-acetylated product was determined by an hplc-fluorimetric procedure. high acs activity was observed in the newly-pupated pupae of both sexes and in the adult male mosquito whose activity was five times that of the female. acetyl coa-dependent n-acetyltransferase (nat; ec 2.3.1.5) activity toward serotonin (5ht) was also st ...19947911372
[effects of nutrient and salinity in egg and larval development of aedes togoi].the study made an observation on periodicity of oviposition, and the effects of nutrient and salinity in egg and larval development of aedes togoi, and the results are summarized as follows: the 53.9% mosquitoes of one feeding laid eggs once, 26.9% laid twice and 19.2% laid three times. autogenous rate of aedes togoi reared in three different nutrient groups in larval sage was 6.9% in 0.8 mg/larva, 22.5% in 1.6 mg/larva and 44.4% in 2.4 mg/larva. the oviposition rate according to different salin ...19957735790
[seasonal prevalence and behaviour of aedes togoi].ecological studies of aedes togoi, the vector of malayan filariasis, were carried out at tolsando, yosu and sokcho area in 1991. the adult population of aedes togoi was continuously appeared from the first week of april to the end of november showing the highest density in july. the larvae of aedes togoi were found at rock pools from march to december in sokcho area and the density was highest in july and august, whereas in the southern coastal area (yosu), the larvae were found throughout the y ...19957735782
geographic variation in the expression of autogeny in aedes togoi (diptera: culicidae) under different temperature and photoperiod conditions.larval developmental time, adult size, and the expression of autogeny were studied in eight populations of aedes togoi (theobald) originating from 4.8 degrees n to 49.2 degrees n under four experimental conditions of temperature and photoperiod (20 degrees c and 10:14 [l:d] h; 20, 25, 30 degrees c and 16:8 [l:d] h). larval developmental time and adult scutum length decreased as a function of increasing rearing temperature. in northern populations at 20 degrees c, larval developmental time was sh ...19957608925
isolation and identification of novel toxins from a new mosquitocidal isolate from malaysia, bacillus thuringiensis subsp. jegathesan.a new mosquitocidal bacillus thuringiensis subsp., jegathesan, has recently been isolated from malaysia. parasporal crystal inclusions were purified from this strain and bioassayed against fourth-instar larvae of culex quinquefasciatus, aedes aegypti, aedes togoi, aedes albopictus, anopheles maculatus, and mansonia uniformis. the 50% lethal concentration of crystal inclusions for each species was 0.34, 8.08, 0.34, 17.59, 3.91, and 120 ng/ml, respectively. these values show that parasporal inclus ...19957487029
genetics of white eye in the mosquito aedes togoi. 19807458469
[on the filariae of the genus aproctella (splendidofilariinae) (author's transl)].aproctella alessandroi n. sp., parasitic in various passeriformes in colombia and guadeloupe, and a. golvani n. sp., a similar species with smaller microfilariae parasitic in sparrows in guadeloupe, are described. the life cycle of a. alessandroi was studied in aedes togoi (development in 13 days in the flight muscles; first-stage larva cylindrical; third-stage larva 1 330-1 600 microns long; oesophago-intestinal junction distinct). the larval biology of aproctella is very similar to that of car ...19817259001
genetics of glucosephosphate isomerase in aedes togoi (diptera: culicidae).the genetics of glucosephosphate isomerase (e.c. 5.3.1.9) in two strains (malaysian and taiwan) of aedes togoi is reported. three electrophoretic phenotypes were presented in both sexes. the zymogram patterns were identical in both strains of a. togoi. the phenotypes were governed by a pair of codominant alleles. the allele frequency of the slow-moving band was 0.63 in the malaysian strain adn was 0,86 and 0.82 in f161 and f169 generations, respectively, of the taiwan strain. the sample studied ...19807225086
studies on the liverpool and malaysian strans of aedes (finlaya) togoi.comparative studies of vector efficiency were done with the liverpool and malaysian strains of aedes (finlaya) togoi for subperiodic brugia malayi and brugia pahangi. the malaysian strain of a. togoi was found to take in fewer microfilariae under the same experimental conditions than the liverpool strain. also, for various microfilarial densities in the host's peripheral blood, the malaysian strain had less mean infective larvae per fed mosquito than the liverpool strain. the microfilarial intak ...19807221700
melanization and encapsulation in aedes aegypti and aedes togoi in response to parasitization by a filarioid nematode (breinlia booliati).in aedes aegypti (singapore strain), s refractory host, there was a marked progressive decline of developing larvae of breinlia booliati during the incubation period. however, in aedes togoi, a susceptible host, the filarial larvae developed normally and the number of larvae remained constant throughout the incubation period. encapsulation and melanization of b. booliati larvae in ae. aegypti and ae. togoi were studied. ae. togoi occasionally mounted a defence reaction to the infection. in ae. a ...19827177704
linkage studies on an esterase locus in the mosquito aedes togoi.an electrophoretic survey of esterases in 7 wild-type and 10 mutant strains of the mosquito aedes (finlaya) togoi was undertaken using thin-layer agar gels. three esterases (designated the est-1, est-2, and est-3 loci in decreasing order of electrophoretic mobility) could be detected from fourth-instar larvae, pupae, and 2- to 5-day-old adults. homogenates of the larvae gave the most intensely stained bands in the gels, especially for est-3. the three esterases were designated carboxylesterases ...19827138496
ultrastructure and chloride cytochemistry of the hindgut epithelium in the larvae of the seawater mosquito, aëdes togoi theobald.the hindgut of aëdes togoi larvae adapted to various external salinities was studied by light and electron microscopy. the larvae's pyloric chamber and the ileum of the hindgut are surrounded by a thick muscular layer and lined with a very thin unspecialized epithelium. there is no detectable difference in the ultrastructural organization of the epithelium between seawater-adapted and freshwater-adapted larvae. the rectum and the anal canal are greatly dilated and densely tracheated and have a v ...19827125862
identification of some common infective filarial larvae in malaysia.infective larvae of wuchereria, brugia, breinlia, dirofilaria and setaria species from an experimental vector, aedes togoi, are compared. the distinctive bubble-like caudal papillae of wuchereria bancrofti are readily distinguishable from the protuberant ones of brugia spp; the 'ear-like' papillae of breinlia are distinct from the 'knob-like' ones of dirofilaria or the 'thorn-like' terminal papilla of setaria.19827069185
genetic linkage relationships between two enzyme loci, est-2 and acph, in the mosquito aedes (finlaya) togoi. 19836870775
experimental transmission of subperiodic wuchereria bancrofti to the leaf monkey (presbytis melalophos), and its periodicity.infective larvae of nocturnally subperiodic wuchereria bancrofti were obtained from laboratory-raised aedes togoi mosquitoes which had fed on an infected human volunteer from kanchanaburi, thailand, and 471 of them were inoculated subcutaneously into the inguinal regions of an immunosuppressed presbytis melalophos. microfilaremia was first detected 287 days after inoculation, and 77 days later, at 364 days of infection, the number of microfilariae determined at 2-hour intervals reached a maximum ...19826805349
genetics of octanol and alpha-glycerophosphate dehydrogenases in the mosquito aedes (finlaya) togoi.genetic studies were performed on octanol dehydrogenase (ec 1.1.1.73) and alpha-glycerophosphate dehydrogenase (ec 1.1.1.8) in the mosquito aedes (finlaya) togoi by agar gel electrophoresis. the electrophoretic survey revealed two octanol dehydrogenase loci (odh-1, odh-2) and one alpha-glycerophosphate dehydrogenase locus (alpha-gpdh) in this species. five alleles were observed at the odh-2 locus in seven laboratory strains, whereas the alpha-gpdh locus was completely monomorphic in six of seven ...19836367734
[preliminary report on the susceptibility of mosquitoes to brugia pahangi].a comparative study on the susceptibility of eight species of four genera of mosquitoes to brugia pahangi was carried out. aedes aegypti (liverpool strain), aedes togoi, armigeres subalbatus and mansonia uniformis (taiwan strains) were found with the third-stage larvae 14 days after feeding on infected rats (sprague-dawley). the remaining of aedes aegypti, aedes albopictus, aedes pseudoalbopictus, culex p. fatigans and culex annutus (taiwan strains) were found refractory to infection. to determi ...19846150813
preliminary observations on the development of larval filariae in toxorhynchites species.brugia malayi and b. pahangi microfilariae from gerbil intraperitoneal infections were inoculated into the thorax of male and female toxorhynchites amboinesis and developed into third-stage larvae as early as 11 days. in a comparative study with aedes togoi fed on microfilaremic gerbils, third-stage larvae were found at 10 days. some third-stage larvae of b. malayi inoculated into gerbils developed to advanced stages. third-stage larvae of wuchereria bancrofti were recovered in low numbers from ...19806101531
studies on filariasis. i. the migration of the infective larvae of brugia pahangi in aedes togoi and their loss from the mosquito under experimental conditions. 19665962368
the development of cardiofilaria nilesi in laboratory-bred aedes togoi. 19695373665
infectivity of brugia malayi larvae recovered from aedes togoi mosquitoes at various times after a single blood meal. 19715153594
the inheritance of autogeny in aedes (finlaya) togoi (theobald) from malaysia and some aspects of its biology. 19725082846
the effects of x-irradiation and antibiotics on the susceptibility of aedes togoi and a. aegypti to subperiodic brugia malayi and b. pahangi. 19694984760
observations on the development of a new filaria (breinlia booliati, singh and ho, 1973) of a rat rattus sabanus in the mosquito aedes togoi. 19734724313
superinfection of aedes togoi with brugia malayi. 19734707759
ultrastructure of anal papillae from a seawater mosquito larva (aedes togoi theobald). 20154696083
development of brugia pahangi in the flight muscles of aedes togoi. ultrastructural changes in the infected muscle fibers and the infecting filarial larvae. 19734688414
the migratory pathway of a filarial parasite (breinlia sergenti) in a mosquito host (aedes togoi). 20034467777
the fate and migratory patterns of the infective larvae of brugia malayi, dirofilaria immitis and breinlia sergenti in aedes togoi denied access to a host. 19744455926
studies on longevity of adult eretmapodites chrysogaster, aedes togoi and aedes (stegomyia) albopictus females (diptera: culicidae). 19694390087
adaptatio of a filarial worm, brugia patei, to a new mosquito host, aedes togoi. 19674383333
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