Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| effect of a lect2 on the immune response of peritoneal lecukocytes against vibrio anguillarum in roughskin sculpin. | leukocyte cell-derived chemotaxin 2 (lect2) is a multi-functional protein that is mainly synthesized by the liver. however, its role in roughskin scalping is less known. here, we cloned a leukocyte cell-derived chemotaxin 2 (tflect2) genes in the liver of roughskin scalping, trachidermus fasciatus, and studied its possible role involved in the immune response against vibrio anguillarum (v. anguillarum) of peritoneal lecukocytes under in vivo conditions. the cdna sequence of tflect2 is 566 bp in ... | 2018 | 29331349 |
| recombinant hsp33 and ompc protein can serve as promising divalent vaccine with protection against vibrio anguillarum and edwardsiella tarda in flounder (paralichthys olivaceus). | vibrio anguillarum and edwardsiella tarda are severe aquaculture pathogens shared similar epidemiological characteristics and susceptible to flounder (paralichthys olivaceus). in our previous studies, recombinant(r) protein heat shock protein 33 (rhsp33) from v. anguillarum and outer membrane protein c (rompc) from e. tarda were proved to have protection against v. anguillarum and e. tarda, respectively. in this paper, the cross protection of rhsp33 against e. tarda and rompc against v. anguilla ... | 2018 | 29309834 |
| role of a macrophage receptor with collagenous structure (marco) in regulating monocyte/macrophage functions in ayu, plecoglossus altivelis. | macrophage receptor with collagenous structure (marco) plays essential roles in phagocytic cell-mediated innate immune responses. however, studies regarding marco, especially its functions, are limited in teleost species. in this study, we identified a marco molecule (pamarco) from ayu (plecoglossus altivelis). pamarco shared conserved functional domains with its mammalian counterparts. sequence analysis showed that pamarco was most closely related to its rainbow trout (oncorhynchus mykiss) coun ... | 2018 | 29305330 |
| endocytosis in adult eel intestine: immunological detection of phagocytic cells in the surface epithelium. | this study was undertaken to isolate groups of eel (anguilla japonica) intestinal cells undergoing active endocytosis in vivo and to localize these cells within the intestinal epithelium. a monoclonal antibody (8f1) of the immunoglobulin m class containing kappa light chains was raised against such endocytic cells. ninetythree percent of the 8f1-positive cells absorbed fluorescent isothiocyanate-conjugated dextran from the intestinal lumen in vivo during a 30-min incubation, and they retained th ... | 1993 | 29300546 |
| molecular characterization and expression analysis of large yellow croaker (larimichthys crocea) interleukin-12a, 16 and 34 after poly i:c and vibrio anguillarum challenge. | interleukin-12, 16 and 34 are important pro-inflammatory cytokines, some of the most important components of the innate immunity system. herein, we identified interleukin-12a (lcil12a), 16 (lcil16) and 34 (lcil34) in large yellow croaker (larimichthys crocea), and determined their expression profile in unchallenged and challenged tissues. the coding sequence (cds) of lcil12a comprised 600 bp long encoding a protein of 199 amino acids (aa), the cds of lcil16 was 2454 bp encoding a protein of 817 ... | 2017 | 29292198 |
| identification of an interferon-stimulated gene, isg15, involved in host immune defense against viral infections in gilthead seabream (sparus aurata l.). | interferons (ifns) play a key role in the innate immunity of vertebrates against viral infections by inducing hundreds of ifn-stimulated genes (isgs), such as isg15. isg15 is an ubiquitin-like protein, which can conjugate cellular and viral proteins in a process called isgylation, although it can also act as a cytokine-like protein. gilthead seabream (sparus aurata l.) is an important asymptomatic carrier of viral haemorrhagic septicaemia virus (vhsv) and nodavirus, representing a threat to othe ... | 2018 | 29277364 |
| amino acids as modulators of the european seabass, dicentrarchus labrax, innate immune response: an in vitro approach. | teleost innate immune system is a most developed and powerful system in which fish highly rely throughout their lives. conditions in aquaculture farms are particularly prone to disease, thus, health and welfare ensuring strategies are an urgent call to which nutrition is gradually becoming a most regarded achievement tool. this study intended to evaluate different amino acids' effect on immune-related mechanisms as well as their potential as enhancers of european seabass, dicentrarchus labrax, l ... | 2017 | 29269876 |
| wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution. | fish skin mucosal surfaces (sms) are quite similar in composition and function to some mammalian ms and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. we aimed to test this hypothesis by searching for metagenomic and genomic evidences in the sms-microbiome of a model fish species (anguilla anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the ... | 2017 | 29268781 |
| the inducible microrna-203 in fish represses the inflammatory responses to gram-negative bacteria by targeting il-1 receptor-associated kinase 4. | innate immune responses are the first defense against pathogenic invaders. activation and termination of these immune responses are regulated by several mechanisms. micrornas (mirnas), a group of small non-coding rnas, have been implicated in the regulation of a spectrum of both physiological and pathological conditions, including immune responses. although immune regulatory mirnas networks in higher vertebrates have been well described, regulation of these responses in fish species is poorly und ... | 2017 | 29242191 |
| peptidoglycan recognition protein of solen grandis (sgpgrp-s1) mediates immune recognition and bacteria clearance. | peptidoglycan recognition proteins (pgrps) are indispensable molecules in innate immunity due to their prominent function in sensing and eliminating invading microorganisms. in the present study, a short type pgrp from razor clam solen grandis (sgpgrp-s1) was recombinantly expressed and purified to investigate its potential function in innate immunity. as a pattern recognition receptor, recombinant sgpgrp-s1 (rsgpgrp-s1) specifically bind lys-type and dap-type peptidoglycan in vitro, but not lip ... | 2018 | 29208495 |
| expression profiles of the p38 mapk signaling pathway from chinese shrimp fenneropenaeus chinensis in response to viral and bacterial infections. | mitogen-activated protein kinase (mapk) signaling pathway plays an important role in cellular response to inflammatory cytokines, environmental stress and pathogenic infection, however, compared with mammals and insects, the potential function of p38 mapk from chinese shrimp (fenneropenaeus chinensis) in response to viruses and bacterial infection is limited. in the study, the immune responses of four genes mkk3, mkk4, p38 and atf-2 from f. chinensis were investigated in defending against white ... | 2018 | 29155327 |
| identification and expression of complement component c8α, c8β and c8γ gene in half-smooth tongue sole (cynoglossus semilaevis) and c8α recombinant protein antibacterial activity analysis. | complement component c8, which mediates membrane attack complex formation and bacterial lysis, plays important roles in the complement system. the cdna sequences of the c8α, c8β and c8γ genes were cloned from half-smooth tongue sole (cynoglossus semilaevis). full-length cdna of csc8α (c8α of c. semilaevis), csc8β and csc8γ was 1990, 2219 and 886 bp, respectively, which contained open reading frames of 1797, 1749 and 666 bp, encoding 598, 582 and 221 amino acids, respectively. the deduced protein ... | 2018 | 29146450 |
| sialic acid-binding lectins (sabls) from solen grandis function as prrs ensuring immune recognition and bacterial clearance. | sialic acid-binding lectins (sabls) are ubiquitous ancient molecules with binding properties to n-acetyl or n-glycolyl carbohydrates, and play crucial roles in both adaptive and innate immune responses. in present study, recombinant protein and antibodies of two sabls from mollusk solen grandis (sgsabl-1 and sgsabl-2) were prepared to investigate their functions in innate immunity. the recombinant protein of sgsabl-1 (rsgsabl-1) could bind lps, pgn and β-glucan in vitro, while rsgsabl-2 could on ... | 2018 | 29146448 |
| isocitrate dehydrogenase mutation in vibrio anguillarum results in virulence attenuation and immunoprotection in rainbow trout (oncorhynchus mykiss). | vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. mutations in central metabolism (glycolysis and the tca cycle) of intracellular pathogens often result in attenuated virulence due to depletion of required metabolic intermediates; however, it was not known whether mutations in central metabolism would affect virulence in an extracellular pathogen such as v. anguillarum. | 2017 | 29137620 |
| immune response of interferon-γ-inducible lysosomal thiol reductase (gilt) from chinese sturgeon (acipenser sinensis) to microbial invasion and its antioxdative activity in lipopolysaccharides-treated mammalian dentritic cells. | interferon-γ-inducible lysosomal thiol reductase (gilt) plays an important role in the major histocompatibility complex-restricted antigen processing of endocytosed proteins via catalyzing the disulfide bond reduction in the endocytic pathway. here, the cdna of chinese sturgeon (acipenser sinensis) gilt (csgilt) was cloned. it contained an open reading frame of 762 nucleotides encoding a protein of 254 amino acids with an estimated molecular weight of 28.1 kda. the characteristic structural feat ... | 2018 | 29133251 |
| molecular characterization of a cc motif chemokine 19-like gene in ayu (plecoglossus altivelis) and its role in leukocyte trafficking. | the cc motif chemokine 19 (ccl19) functions in acute inflammation by recruiting lymphocytes and other cells. however, ccl19 has only been investigated in few fish species. in this study, we characterized a ccl19-like molecule (paccl19l) in ayu (plecoglossus altivelis), a teleost fish. sequence analysis revealed that paccl19l was most closely related to atlantic salmon (salmon salar) ccl19l1, which belonged to the fish ccl19a.1 subcluster. paccl19l was constitutively expressed in the tested ayu t ... | 2018 | 29128493 |
| potential outer membrane protein candidates for vaccine development against the pathogen vibrio anguillarum: a reverse vaccinology based identification. | reverse vaccinology is a widely used approach that has facilitated the rapid identification of vaccine candidates suitable in vaccine development for pathogens. vibrio anguillarum is a major pathogen responsible for vibriosis in fish and shellfish leading to huge economic losses to the aquaculture industry. although commercial vaccines are available for fish against this bacterium they have their own limitations. in this study, we used the reverse vaccinology strategy to screen and identify v. a ... | 2017 | 29119233 |
| salinity-based toxicity of cuo nanoparticles, cuo-bulk and cu ion to vibrio anguillarum. | bacteria are used in ecotoxicology for their important role in marine ecosystems and their quick, reproducible responses. here we applied a recently proposed method to assess the ecotoxicity of nanomaterials on the ubiquitous marine bacterium vibrio anguillarum, as representative of brackish and marine ecosystems. the test allows the determination of 6-h ec50 in a wide range of salinity, by assessing the reduction of bacteria actively replicating and forming colonies. the toxicity of copper oxid ... | 2017 | 29118743 |
| poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of european sea bass yolk-sac larvae. | the reliable production of marine fish larvae is one of the major bottlenecks in aquaculture due to high mortalities mainly caused by infectious diseases. to evaluate if the compound poly-β-hydroxybutyrate (phb) might be a suitable immunoprophylactic measure in fish larviculture, its capacity to improve immunity and performance in european sea bass (dicentrarchus labrax) yolk-sac larvae was explored. phb was applied from mouth opening onwards to stimulate the developing larval immune system at t ... | 2017 | 29118332 |
| two novel calreticulin-related molecules with microbial binding and phagocytosis enhancing capacity in the half-smooth tongue sole, cynoglossus semilaevis. | calreticulin (crt) is highly conserved chaperone located in the endoplasmic reticulum. it plays important roles in innate immunity. although various immune-related functions of crt have been reported in vertebrates and invertebrates, information on the potential functions of teleost crt is very limited. in the present study, we characterized two calreticulin-related molecules from tongue sole (cynoglossus semilaevis), calreticulin-like1 and calreticulin-like2 (cscrtl1 and cscrtl2). cscrtl1and cs ... | 2018 | 29104090 |
| isolation and characterization of bacteria colonizing acartia tonsa copepod eggs and displaying antagonist effects against vibrio anguillarum, vibrio alginolyticus and other pathogenic strains. | copepods represent a major source of food for many aquatic species of commercial interest for aquaculture such as mysis shrimp and early stages of fishes. for the purpose of this study, the culturable mesophilic bacterial flora colonizing acartia tonsa copepod eggs was isolated and identified. a total of 175 isolates were characterized based on their morphological and biochemical traits. the majority of these isolates (70%) were gram-negative bacteria. matrix-assisted laser desorption/ionization ... | 2017 | 29085344 |
| characterization of a double wap domain-containing protein from the red swamp crayfish procambarus clarkii. | crustaceans express multiple whey acidic protein (wap) domain containing proteins which are components of host immunity. in the present study, a new double wap domain containing protein was identified from red swamp crayfish procambarus clarkii, designated pc-dwd. the orf is 387 bp, encoding 128 amino acids consisting of signal peptide of 18 residues, and two tandem wap domains of 38 and 44 residues. multiple alignment indicates the presence of conserved motifs in both wap domains, and phylogene ... | 2017 | 29054827 |
| zinc finger protein 365 is a new maternal lps-binding protein that defends zebrafish embryos against gram-negative bacterial infections. | zinc finger protein 365 (znf365) is widespread in animals, but its function and mechanism remains poorly defined. here we clearly demonstrate that zebrafish znf365 is a newly identified lps-binding protein capable of interacting with the gram-negative bacteria escherichia coli, vibrio anguillarum, and aeromonas hydrophila, and functions as an antibacterial effector molecule capable of directly killing the bacteria. we also reveal that n-terminal residues 30-55 consisting of the znf_c2h2 domain a ... | 2017 | 29042450 |
| immunological responses and disease resistance of rainbow trout (oncorhynchus mykiss) juveniles following dietary administration of stinging nettle (urtica dioica). | the present study investigated the effects of dietary supplementation of stinging nettle (urtica dioica) on growth performance, skin mucus, immune response and disease resistance of rainbow trout (oncorhynchus mykiss) fed with diets supplemented with u. dioica at 0, 1, 2 and 3%. after 8 weeks of feeding, the addition of u. dioica at 3% level resulted in improved weight gain, specific growth rate and feed conversion ratio significantly when compared to the other groups (p < 0.05). hematological r ... | 2017 | 29017944 |
| activation of toll pathway is different between kuruma shrimp and drosophila. | the toll pathway is essential for inducing an immune response to defend against bacterial invasion in vertebrates and invertebrates. although toll receptors and the transcription factor dorsal were identified in different shrimp, relatively little is known about how the toll pathway is activated or the function of the pathway in shrimp antibacterial immunity. in this study, three tolls (toll1-3) and the dorsal were identified in marsupenaeus japonicus. the toll pathway can be activated by gram-p ... | 2017 | 28979261 |
| transcriptome mining of immune-related genes in the muricid snail concholepas concholepas. | the population of the chilean endemic marine gastropod concholepas concholepas locally called "loco" has dramatically decreased in the past 50 years as a result of intense activity of local fisheries and high environmental variability observed along the chilean coast, including episodes of hypoxia, changes in sea surface temperature, ocean acidification and diseases. in this study, we set out to explore the molecular basis of c. concholepas to cope with biotic stressors such as exposure to the p ... | 2017 | 28962882 |
| the tlr13-myd88-nf-κb signalling pathway of cyclina sinensis plays vital roles in innate immune responses. | toll-like receptors, the best known pattern recognition receptors, play important roles in recognizing non-self molecules and binding pathogen-associated molecular patterns in the innate immune system. in the present research, the cdna and protein characterization of the tlr signalling pathway genes including irak4, trak6 and ikkα (named csirak4, cstraf6 and csikkα, respectively) with the typical motifs from cyclina sinensis showed significant similarity with their homologues from other shellfis ... | 2017 | 28958897 |
| stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in vibrio anguillarum against cold stress. | the capability of cold-adaptation is a prerequisite of microorganisms that survive in an environment with frequent fluctuations in temperature. as a global causative agent of vibriosis in marine fish farming, vibrio anguillarum can efficiently grow and proliferate under cold-stress conditions, which is 15°c lower than the optimal growth temperatures (25-30°c). our data showed that v. anguillarum was able to synthesize ectoine de novo and that ectoine was essential for its growth under cold stres ... | 2017 | 28942848 |
| expression and functional characterization of interferon regulatory factors 4, 8, and 9 in large yellow croaker (larimichthys crocea). | interferon regulatory factor (irf)-4, 8, and 9 are essential in host defense against pathogens. here, the full-length coding sequence (cds), protein structure, and immune response of irf4/8/9 (lc irf4/8/9) were characterized in large yellow croaker (larimichthys crocea). the open reading frame of lcirf4, lcirf8 and lcirf9 encoded putative proteins of 463,422 and 406 amino acids, respectively. these irfs share high sequence homology with other vertebrate irfs and were constitutively expressed in ... | 2018 | 28928075 |
| comparative study of three c1q domain containing proteins from pacific oyster crassostrea gigas. | c1q domain containing proteins (c1qdcs) are a family of proteins containing a globular head c1q domain (ghc1q) in c-terminus, which serve as pattern recognition receptors (prrs) and mediate a series of immune responses. in the present study, three c1qdc proteins from pacific oyster crassostrea gigas (cgc1qdc-2, cgc1qdc-3, cgc1qdc-4) were characterized and comparatively investigated to understand their roles in the immune response. all the three recombinant cgc1qdc proteins (rcgc1qdcs) could bind ... | 2018 | 28923592 |
| pfma, a novel quorum-quenching n-acylhomoserine lactone acylase from pseudoalteromonas flavipulchra. | many bacteria, such as proteobacteria, cyanobacteria and bacteroidetes, use n-acylhomoserine lactones (ahls) as quorum-sensing (qs) signal molecules for communication. enzymatic degradation of ahls, such as ahl acylase and ahl lactonase, can degrade ahls (quorum quenching, qq) to attenuate or disarm the virulence of pathogens. qq is confirmed to be common in marine bacterial communities. many genes encoding ahl acylases are found in marine bacteria and metagenomic collections, but only a few of ... | 2017 | 28920855 |
| two novel lrr and ig domain-containing proteins from oyster crassostrea gigas function as pattern recognition receptors and induce expression of cytokines. | leucine-rich repeat (lrr) domain and immunoglobulin (ig) domain are both competent immune recognition modules, and the immunological roles of lrr and ig domain containing- proteins (lrrigs) are speculated to be multifunctional and worth investigating. in the present study, two novel lrrigs, cglrrig-1 and cglrrig-2, were identified and characterized from oyster crassostrea gigas. both of them contained an n-terminal lrr region, an ig domain, a transmembrane region, and a c-terminal cytoplasmic ta ... | 2017 | 28889011 |
| modulatory in vitro effect of stress hormones on the cytokine response of rainbow trout and gilthead sea bream head kidney stimulated with vibrio anguillarum bacterin. | in fish, the stress response and their consequences in the immune system have been widely described. recently, a differential cytokine regulation between rainbow trout (oncorhynchus mykiss) and gilthead sea bream (sparus aurata) was reported after treatment with stress hormones together with their receptor antagonists. nevertheless, there is no evidence of whether antagonists for stress hormone receptors may influence the interaction between hormones and cytokines after bacterial administration. ... | 2017 | 28882798 |
| molecular characterization, expression and functional analysis of two kazal-type serine protease inhibitors from venerupis philippinarum. | kazal-type serine protease inhibitors (kspis) act as negative regulators in immune signaling pathway by controlling the extent of serine protease (sp) activities. in this study, the full-length cdna of two kspis (designed as vpkspi-1 and vpkspi-2) were identified from venerupis philippinarum by rapid amplification of cdna ends (race) approaches. the open reading frame (orf) of vpkspi-1 and vpkspi-2 was of 552 bp and 402 bp, encoding a polypeptide of 183 and 133 amino acids, respectively. the tra ... | 2017 | 28882790 |
| ocean acidification and pathogen exposure modulate the immune response of the edible mussel mytilus chilensis. | ocean acidification (oa) is one of the main consequences of increasing atmospheric carbon dioxide (co2), impacting key biological processes of marine organisms such as development, growth and immune response. however, there are scarce studies on the influence of oa on marine invertebrates' ability to cope with pathogens. this study evaluated the single and combined effects of oa and bacterial infection on the transcription expression of genes related to antioxidant system, antimicrobial peptides ... | 2017 | 28870859 |
| molecular characterization and expression analysis of the large yellow croaker (larimichthys crocea) chemokine receptors cxcr2, cxcr3, and cxcr4 after bacterial and poly i:c challenge. | the large yellow croaker (larimichthys crocea) has a well-developed innate immune system. we studied a component of this system, chemokine receptor cxcr family. in this study, we report the full-length open reading frames, as well as the identification and characterization of the chemokine receptor genes cxcr2 (lyccxcr2), cxcr3 (lyccxcr3), and cxcr4 (lyccxcr4) of large yellow croaker. we report that lyccxcr3 and lyccxcr4 are evolving neutrally according to paml analyses. quantitative real-time p ... | 2017 | 28870858 |
| evaluation of antibody response to an adjuvanted hapten-protein vaccine as a potential inhibitor of sexual maturation for farmed atlantic salmon. | an experimental contraceptive vaccine was evaluated in atlantic salmon (salmo salar). a peptide derived from the beta subunit of luteinizing hormone (lh) was conjugated to two different carrier proteins, bovine serum albumin (bsa) and keyhole limpet hemocyanin (klh), and formulated with one of four immunostimulants in a water-in-oil emulsion. specific antibody responses to the peptide and each carrier protein were evaluated. while the antibody response to klh was stronger than the response to bs ... | 2017 | 28866277 |
| the versatile functions of lrr-only proteins in mollusk chlamys farreri. | leucine-rich repeat (lrr)-only proteins are involved in the innate immune responses as they mediate protein-ligand interactions. in the present study, three novel lrr-only proteins, cflrrop-4, cflrrop-5 and cflrrop-6, were identified and characterized from zhikong scallop chlamys farreri. they all contained lrr motifs with consensus signature sequences of lxxlxlxxnxl or lxxlxlxxcxxl. all the mrna transcripts of three cflrrops were high abundant in hepatopancreas, gills and gonads, and their mrna ... | 2017 | 28807724 |
| primary culture of zhikong scallop chlamys farreri hemocytes as an in vitro model for studying host-pathogen interactions. | primary cultured cells can be a useful tool in studies on physiology, virology, and toxicology. hemocytes play an important role in animal rapid response to pathogen invasion. in this study, an appropriate medium for primary culture of hemocytes of the bivalve chlamys farreri was developed by adding 5% fetal bovine serum and 1% c. farreri serum to leibovitz l-15 medium. these primary cultured hemocytes were maintained for more than 40 d in vitro and were classified into 3 types: (1) granulocytes ... | 2017 | 28792420 |
| iron acquisition strategies of vibrio anguillarum. | the hemorrhagic septicemic disease vibriosis caused by vibrio anguillarum shows noticeable similarities to invasive septicemia in humans, and in this case, the v. anguillarum-host system has the potential to serve as a model for understanding native eukaryotic host-pathogen interactions. iron acquisition, as a fierce battle occurring between pathogenic v. anguillarum and the fish host, is a pivotal step for virulence. in this article, advances in defining the roles of iron uptake pathways in gro ... | 2017 | 28791260 |
| interplay between the gut microbiota and immune responses of ayu (plecoglossus altivelis) during vibrio anguillarum infection. | gut microbiota plays fundamental roles in protection against pathogen invasion. however, the mechanism and extent of responses of gut microbiota to pathogenic infection are poorly understood. this study investigated the gut bacterial communities and immune responses of ayu (plecoglossus altivelis) upon exposure to vibrio anguillarum. the succession of v. anguillarum infection was evidenced by increased expression of immune genes and bacterial loads in ayu tissues, which in turn altered the compo ... | 2017 | 28756287 |
| molecular characterization and functional analysis of the hepcidin gene from roughskin sculpin (trachidermus fasciatus). | hepcidin is a kind of cysteine-rich antimicrobial peptide that plays a vital role in host innate immune activity and iron regulation. here, we report the molecular characterization and functional analysis of a novel hamp1 hepcidin isoforms tf-hep from roughskin sculpin, trachidermus fasciatus. a cdna fragment of 988 bp with an orf of 273 bp was obtained. the coding sequence encodes for a signal peptide of 24 amino acids coupled with a prodomain of 40 amino acids and a mature peptide of 26 amino ... | 2017 | 28743631 |
| novel mannose binding natterin-like protein in the skin mucus of atlantic cod (gadus morhua). | this study presents the first report of purification of natterin-like protein (nlp) in a non-venomous fish. the peptide identities of purified cod nlp were confirmed through lc-msms and matched to a cod expressed sequence tag (est). a partial cod nlp nucleotide sequence was amplified and sequenced based on this est. multiple sequence alignment of cod nlp showed considerable homology with other teleost nlps and the presence of an n-terminal jacalin-like lectin domain coupled with a c-terminal tox ... | 2017 | 28743623 |
| identification and expression analysis of fetuin b (fetub) in turbot (scophthalmus maximus l.) mucosal barriers following bacterial challenge. | fetuin b (fetub), a recently described cysteine proteinase inhibitor, has numerous conserved n-glycosylation sites, species-specific o-glycosylation sites, and two cystatin (cy) domains. fetub is likely to play regulatory roles in acute inflammation, female infertility, fish organogenesis and tumor suppression. in the present study, transcript of turbot fetub gene was captured, its protein structure and expression patterns in different tissues with emphasis on mucosal barriers following differen ... | 2017 | 28732765 |
| identification, characterization and expression analysis of tlr5 in the mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | tlrs (toll-like receptors) are very important pathogen pattern recognition receptors, which control the host immune responses against pathogens through recognition of molecular patterns specific to microorganisms. in this regard, investigation of the turbot tlrs could help to understand the immune responses for pathogen recognition. here, transcripts of two tlr5 (tlr5a and tlr5b) were captured, and their protein structures were also predicted. meanwhile, we characterized their expression pattern ... | 2017 | 28705722 |
| zebrafish (danio rerio) as a model to visualize infection dynamics of vibrio anguillarum following intraperitoneal injection and bath exposure. | vaccine development is important for sustainable fish farming and novel vaccines need to be efficacy tested before release to the market. challenge of fish with the pathogen towards which the vaccine has been produced can be conducted either by external exposure though bathing or cohabitation, or by bypassing the mucosa through injection. the latter approach is often preferred since it is easier to control than the former. however, injection is not a very natural route of infection, and the bypa ... | 2017 | 28663130 |
| crystal structures of the isochorismatase domains from vibrio anguillarum. | antibiotic resistance is becoming a global threat and overuse of antibiotics in aquaculture disease control worsens the situation. to reduce the risk of drug resistance developed in aquaculture, safer biocontrol programs are needed. antivirulence therapy, with less chance for developing drug resistance, is a promising approach. to facilitate antivirulence inhibitor design against vibrio anguillarum, a serious aquaculture pathogen, we present crystal structures for isochorismatase domains of angb ... | 2017 | 28647364 |
| ecotoxicological method with marine bacteria vibrio anguillarum to evaluate the acute toxicity of environmental contaminants. | bacteria are an important component of the ecosystem, and microbial community alterations can have a significant effect on biogeochemical cycling and food webs. toxicity testing based on microorganisms are widely used because they are relatively quick, reproducible, cheap, and are not associated with ethical issues. here, we describe an ecotoxicological method to evaluate the biological response of the marine bacterium vibrio anguillarum. this method assesses the acute toxicity of chemical compo ... | 2017 | 28605381 |
| disease resistance and response against vibrio anguillarum intestinal infection in european seabass (dicentrarchus labrax) fed low fish meal and fish oil diets. | the aim of this study was to assess the effects of low levels of dietary fish meal (fm) and fish oil (fo) on disease resistance and gut associated lymphoid tissue (galt) response after an experimental intestinal infection with v. anguillarum in european sea bass (dicentrarchus labrax) for that purpose, sea bass juveniles were fed one of four diets containing combined levels of fo and fm as follows: 20%fm/6%fo, 20%fm/3%fo, 5%fm/6%fo and 5%fm/3%fo during 153 days. at the end of the feeding trial, ... | 2017 | 28602741 |
| scavenger receptor c promotes bacterial clearance in kuruma shrimp marsupenaeus japonicus by enhancing hemocyte phagocytosis and amp expression. | scavenger receptors (srs) comprise a large family of structurally diverse glycoproteins located on the cell membrane and function as pattern-recognition receptors (prrs) participating in innate immunity in different species. class c scavenger receptor (src) has been only identified in invertebrates and its biological functions still need to be researched. in this study, we characterized the anti-bacterial function of a src from kuruma shrimp marsupenaeus japonicus (mjsrc). the mrna level of mjsr ... | 2017 | 28602682 |
| properties of probiotics kocuria sm1 and rhodococcus sm2 isolated from fish guts. | this study characterized probiotics kocuria sm1 and rhodococcus sm2, which were recovered from the intestinal microbiota of rainbow trout (oncorhynchus mykiss, walbaum). the cultures were gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. cell multiplication of sm1 and sm2 was observed at 4-37 °c (45 °c for sm1), in 0-20% (w/v) nacl and at ph 2-11. the viability was not affected when exposed to pepsin at ph 2.0 and 3.0, and pancreatin at ph 8.0. neither isolates wer ... | 2017 | 28585158 |
| studies on the antibody response and side effects after intramuscular and intraperitoneal injection of atlantic lumpfish (cyclopterus lumpus l.) with different oil-based vaccines. | atlantic lumpfish (cyclopterus lumpus l.) is used as a biological delousing agent for sea lice (lepeophtheirus salmonis k.) infestations in norwegian aquaculture. here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. both vaccines contained bacterial antigens from atypical aeromonas salmonicida a-layer types v and vi, vibrio anguillarum serotype o1 and moritella viscosa sp., but one vaccine cont ... | 2017 | 28548686 |
| mirna-8159 is involved in tlr signaling pathway regulation after pathogen infection by direct targeting tlr13 in miiuy croaker. | toll-like receptors (tlrs) play a crucial role in the recognition of immune reactions against invading pathogens. the molecular regulation mechanisms of tlr expression in aquatic organisms remain unclear. micrornas (mirnas) are small non-coding rnas that are critical adjustors of immune signaling pathway at the post-transcriptional level and play critical roles in intricate networks of host-pathogen interactions and innate immunity. the critical role of tlrs in host defense for discerning certai ... | 2017 | 28546024 |
| stumbling across the same phage: comparative genomics of widespread temperate phages infecting the fish pathogen vibrio anguillarum. | nineteen vibrio anguillarum-specific temperate bacteriophages isolated across europe and chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. the phages were classified as siphoviridae with genome sizes between 46,006 and 54,201 bp. all 19 phages showed high genetic similarity, and 13 phages were genetically identical. apart from sporadically distributed single nucleotide polymorphisms (snps), genetic diversi ... | 2017 | 28531104 |
| in vitro effects of italian lavandula multifida l. leaf extracts on gilthead seabream (sparus aurata) leucocytes and saf-1 cells. | lavandula multifida is very appreciated by pharmaceutical and cosmetic industries. in italy is only found in calabria and sicily and, at present, urge its valorization due to its high extinction and genetic erosion risks. possible applications of l. multifida extracts as immunostimulant in fish aquaculture were assayed by using gilthead seabream (sparus aurata) as a marine fish model, due to its importance in fish aquaculture. the in vitro effects of both aqueous and ethanolic leaf extracts obta ... | 2017 | 28522420 |
| the involvement of cathepsin f gene (ctsf) in turbot (scophthalmus maximus l.) mucosal immunity. | cathepsin f (ctsf) is a recently described papain-like cysteine protease and unique among cathepsins due to an elongated n-terminal pro-region, which contains a cystatin domain. ctsf likely plays a regulatory role in processing the invariant chain which is associated with the major histocompatibility complex (mhc) class ii. in this regard, we identified the ctsf gene of turbot as well as its protein structure, phylogenetic relationships, and expression patterns in mucosal tissues following vibri ... | 2017 | 28501446 |
| identification of immunogenic proteins and evaluation of four recombinant proteins as potential vaccine antigens from vibrio anguillarum in flounder (paralichthys olivaceus). | vibrio anguillarum is a severe bacterial pathogen that can infect a wide range of fish species. identification of immunogenic proteins and development of vaccine are essential for disease prevention. in this study, immunogenic proteins were screened and identified from v. anguillarum, and then protective efficacy of the immunogenic proteins was evaluated. immunogenic proteins in v. anguillarum whole cell were detected by western blotting (wb) using immunized flounder (paralichthys olivaceus) ser ... | 2017 | 28483195 |
| defense systems in developing artemia franciscana nauplii and their modulation by probiotic bacteria offer protection against a vibrio anguillarum challenge. | the alterations of immune responses of artemia franciscana nauplii as a function of culture time and after a challenge with the pathogen vibrio anguillarum were studied. the effect of the administration of the probiotic bacteria bacillus subtilis, lactobacillus plantarum and lactococcus lactis either alone or in combination with the pathogen was evaluated. the activity of the antioxidant enzymes superoxide dismutase (sod), glutathione reductase (gred), glutathione transferase (gst) and phenoloxi ... | 2017 | 28478257 |
| functional diets modulate the acute phase protein response in oncorhynchus mykiss subjected to chronic stress and challenged with vibrio anguillarum. | the acute phase response to pathogens alters the production of proinflammatory cytokines that, in turn, activate the synthesis of acute phase proteins. these proteins neutralize, prevent, and indicate tissue damage, thereby influencing the specific immune response and allowing the organism to regain homeostasis. functional diets based in pre- and probiotics are used in aquaculture to improve fish health and resistance to diseases, but there is an information gap on the mechanisms involved in the ... | 2017 | 28476670 |
| the polymorphism of chicken-type lysozyme gene in japanese flounder (paralichthys olivaceus) and its association with resistance/susceptibility to listonella anguillarum. | lysozyme is a crucially spread hydrolase in organisms that can defend against bacterial infection in innate immunity. in this study, we successfully sequenced the coding region of chicken-type lysozyme gene (polysc) in paralichthys olivaceus and identified nine single nucleotide polymorphisms (snps). we then amplified the 2500 bp promoter region of lysozyme and identified the eight sites of polymorphisms. all snps were genotyped between susceptible and resistance groups after listonella anguilla ... | 2017 | 28476668 |
| crude oil impairs immune function and increases susceptibility to pathogenic bacteria in southern flounder. | exposure to crude oil or its individual constituents can have detrimental impacts on fish species, including impairment of the immune response. increased observations of skin lesions in northern gulf of mexico fish during the 2010 deepwater horizon oil spill indicated the possibility of oil-induced immunocompromisation resulting in bacterial or viral infection. this study used a full factorial design of oil exposure and bacterial challenge to examine how oil exposure impairs southern flounder (p ... | 2017 | 28464028 |
| dextran production by lactobacillus sakei mn1 coincides with reduced autoagglutination, biofilm formation and epithelial cell adhesion. | in this work we have investigated two dextran-producing lactic acid bacteria, lactobacillus sakei mn1 and leuconostoc mesenteroides rtf10, isolated from fermented meat products. these bacteria synthesise dextran when sucrose, but not glucose, is present in the growth medium. the influence of dextran on bacterial aggregation, adhesion and biofilm formation was investigated in cultures challenged with sucrose or glucose. for lb. sakei mn1, the synthesis of the dextran drastically impaired the thre ... | 2017 | 28457443 |
| molecular characterization and antibacterial activity of a phage-type lysozyme from the manila clam, ruditapes philippinarum. | a phage-type lysozyme, designed as rpplyz, was cloned and characterized from the clam ruditapes philippinarum. the full-length cdna of rpplyz was of 699 bp with an open reading frame (orf) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kda and an isoelectric point of 9.05. multiple alignments and phylogenetic analysis strongly suggested that rpplyz was a new member of the phage-type lysozyme family. the mrna transcript of rpplyz was found to be const ... | 2017 | 28365385 |
| protective efficacy of six immunogenic recombinant proteins of vibrio anguillarum and evaluation them as vaccine candidate for flounder (paralichthys olivaceus). | vibrio anguillarum is a severe bacterium that causes terminal haemorrhagic septicaemia in freshwater and marine fish. virulence-associated proteins play an important role in bacterial pathogenicity and could be applied for immunoprophylaxis. in this study, six antigenic proteins from v. anguillarum were selected and the immune protective efficacy of their recombinant proteins was investigated. vira, cher, flac, ompk, ompr and hsp33 were recombinantly produced and the reactions of recombinant pro ... | 2017 | 28351709 |
| molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam venerupis philippinarum. | lysozymes play an important role in the innate immune responses with which mollusks respond to bacterial invasion through its lytic activity. in the present study, two c-type lysozymes (designed as vpclyz-1 and vpclyz-2, respectively) were identified and characterized from the manila clam venerupis philippinarum. the full-length cdna of vpclyz-1 and vpclyz-2 was of 629 and 736 bp, encoding a polypeptide of 156 and153 amino acid residues, respectively. the deduced amino acid sequences of vpclyzs ... | 2017 | 28344168 |
| identification and characterization of a novel calreticulin involved in the immune response of the zhikong scallop, chlamys farreri. | calreticulin (crt) is a multifunctional calcium-binding chaperone shared among vertebrates and invertebrates. in this study, a novel crt (cfcrt) was identified in the zhikong scallop chlamys farreri by rapid amplification of cdna ends. the full-length cdna was composed of 1345 bp, which included a 1158 bp open reading frame, a 25 bp 5'-untranslated region (utr) and a 162 bp 3'-utr. the predicted molecular mass of cfcrt was 44.8 kda. cfcrt contained three highly conserved domains (n-, p- and c-do ... | 2017 | 28323215 |
| genome-guided transcriptome analysis of miiuy croaker provides insights into pattern recognition receptors and cytokines in response to vibrio anguillarum. | miiuy croaker (miichthys miiuy), as an economically important marine fish is affected by numerous bacterial diseases. infection by bacterial pathogen vibrio anguillarum causes high mortality and great economic loss in aquaculture. to understand the immune response of the miiuy croaker to v. anguillarum infection, we analyzed the transcriptomic profile of v. anguillarum-challenged m. miiuy, and two cdna libraries, namely, the normal library (ctrl) and v. anguillarum challenged library (van) were ... | 2017 | 28315364 |
| comparative genome analyses of vibrio anguillarum strains reveal a link with pathogenicity traits. | vibrio anguillarum is a marine bacterium that can cause vibriosis in many fish and shellfish species, leading to high mortalities and economic losses in aquaculture. although putative virulence factors have been identified, the mechanism of pathogenesis of v. anguillarum is not fully understood. here, we analyzed whole-genome sequences of a collection of v. anguillarum strains and compared them to virulence of the strains as determined in larval challenge assays. previously identified virulence ... | 2017 | 28293680 |
| antagonistic activity and mode of action of phenazine-1-carboxylic acid, produced by marine bacterium pseudomonas aeruginosa pa31x, against vibrio anguillarum in vitro and in a zebrafish in vivo model. | phenazine and its derivatives are very important secondary metabolites produced from pseudomonas spp. and have exhibited broad-spectrum antifungal and antibacterial activities. however, till date, there are few reports about marine derived pseudomonas and its production of phenazine metabolites. in this study, we isolated a marine pseudomonas aeruginosa strain pa31x which produced natural product inhibiting the growth of vibrio anguillarum c312, one of the most serious bacterial pathogens in mar ... | 2017 | 28289406 |
| characterization and expression analysis of chitinase genes (chit1, chit2 and chit3) in turbot (scophthalmus maximus l.) following bacterial challenge. | chitinases are hydrolytic enzymes which have been employed to breakdown chitin coats of pathogenic microorganisms, thereby weaken the defense system of several pathogens and insects. in this regard, we identified the chitinase genes of turbot and characterized their expression patterns in mucosal tissues following vibrio anguillarum and streptococcus iniae challenge. in present study, transcripts of three chitinase genes (chit1, chit2 and chit3) were captured, as well as their protein structures ... | 2017 | 28286313 |
| establishment and characterization of a cell line from the head kidney of golden pompano trachinotus ovatus and its application in toxicology and virus susceptibility. | a cell line derived from the head kidney of golden pompano trachinotus ovatus (tohk) was established and characterized in this study. the tohk cells grew most rapidly at 28° c and the optimum foetal bovine serum concentration in l-15 medium was 10%. the tohk cells have a diploid chromosome number of 2n = 54. the transfection efficiency of tohk cells was 7·5% at the 15th passage and 72% at the 40th passage. the transfection efficiency in tohk cells was high, so these cells are suitable for foreig ... | 2017 | 28271507 |
| transcriptome analysis of ruditapes philippinarum hepatopancreas provides insights into immune signaling pathways under vibrio anguillarum infection. | the manila clam, ruditapes philippinarum, is one of the most economically important aquatic clams that are harvested on a large scale by the mariculture industry in china. however, increasing reports of bacterial pathogenic diseases have had a negative effect on the aquaculture industry of r. philippinarum. in the present study, the two transcriptome libraries of untreated (termed h) and challenged vibrio anguillarum (termed hv) hepatopancreas were constructed and sequenced from manila clam usin ... | 2017 | 28267631 |
| microrna-3570 modulates the nf-κb pathway in teleost fish by targeting myd88. | the inflammatory response, a protective process to clear detrimental stimuli, constitutes the defense against infectious pathogens. however, excessive inflammation disrupts immune homeostasis, which may induce autoimmune and inflammatory diseases. in this study, we report that microrna (mir)-3570 plays a negative role in the bacteria-induced inflammatory response of miiuy croaker. upregulation of mir-3570 by vibrio anguillarum and lps inhibits lps-induced inflammatory cytokine production, thus a ... | 2017 | 28250156 |
| characterization and expression analysis of serpinb3, the first clade b serine protease inhibitor in pacific white shrimp, litopenaeus vannamei. | currently, about nine serpin clades (a-i) were preferentially observed in higher animals and clustered on the basis of function. of these, eight clades contain extracellular proteins, while clade b contains predominantly intracellular proteins. in the present study, the first clade b serpin (named lvserpinb3) was identified from the pacific white shrimp litopenaeus vannamei. lvserpinb3 encoded a 412-amino acid protein with a 19-amino acid signal peptide and a serpin domain. moreover, a transmemb ... | 2017 | 28238878 |
| amoebic gill disease outbreak in marine fish cultured in korea. | in 2015, 6.7-60% mortality was observed in black seabream ( acanthopagrus schlegelii), rock bream ( oplegnathus fasciatus), and gray mullet ( mugil cephalus) farmed in the southern coast of korea. on examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. some rock bream and gray mullet were coinfected with bacteria ( pseudomonas anguilliseptica, vibrio tapetis, or vibrio anguillarum). histologic examination revealed extensive hyperplastic epith ... | 2017 | 28205457 |
| histamine and mast cell activator compound 48/80 are safe but inefficient systemic adjuvants for gilthead seabream vaccination. | histamine has a key role in the regulation of inflammatory and innate immune responses in vertebrates. gilthead seabream (sparus aurata l.), a marine hermaphrodite teleost of great commercial value, was the first fish species shown to possess histamine-containing mast cells (mcs) at mucosal tissues. mcs are highly abundant in the peritoneal exudate of gilthead seabream and compound 48/80 (co 48/80), often used to promote mc activation and histamine release, is able to promote histamine release f ... | 2017 | 28193449 |
| comparative assessment of vibrio virulence in marine fish larvae. | vibrionaceae infections are a major obstacle for marine larviculture; however, little is known about virulence differences of vibrio strains. the virulence of vibrio strains, mostly isolated from vibriosis outbreaks in farmed fish, was tested in larval challenge trials with cod (gadus morhua), turbot (scophthalmus maximus) and halibut (hippoglossus hippoglossus) using a multiwell dish assays with single-egg/larvae cultures. the strains differed significantly in virulence as some caused a high mo ... | 2017 | 28160295 |
| rainbow trout (oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens. | commensal bacteria co-exist on the mucosal surfaces of all vertebrates. the host's mucosal immune system must tolerate commensals while fighting pathogens. one of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pigr). rainbow trout pigr is known to transport igt and igm across epithelia. however, other biological functions for trout pigr or t ... | 2017 | 28150752 |
| molecular identification and functional analysis of klf2 in plecoglossus altivelis (ayu): it's regulatory role in monocyte/macrophage activation. | monocytes/macrophages (mo/mф) play an important role in the response to infection in plecoglossus altivelis (ayu). however, the role of transcription factors in the function of ayu mo/mф is poorly understood. here, we cloned the cdna sequence of the kruppel-like factor 2 (paklf2) gene from ayu. phylogenetic analysis indicated that paklf2 was closest to that of atlantic salmon (salmo salar). real time quantitative pcr (rt-qpcr) revealed that the paklf2 mrna level was highest in the peripheral blo ... | 2017 | 28130078 |
| two short peptidoglycan recognition proteins from crassostrea gigas with similar structure exhibited different pamp binding activity. | peptidoglycan recognition protein (pgrp) is an essential molecule in innate immunity for both invertebrates and vertebrates, owing to its prominent ability in specifically recognizing bacterial peptidoglycan (pgn) and eliminating the invading bacteria. in the present study, the full length cdna of two pgrp genes, cgpgrps2 and cgpgrps4, were cloned from oyster crassostrea gigas. their amino acid sequences both contained one signal peptide, one typical pgrp/amidase domain with conserved catalytic ... | 2017 | 28042081 |
| biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen vibrio anguillarum. | fish pathogen vibrio anguillarum, a mesophile bacterium, is usually found in estuarine and marine coastal ecosystems worldwide that pose a constant stress to local organism by its fluctuation in salinity as well as notable temperature change. though v. anguillarum is able to proliferate while maintain its pathogenicity under low temperature (5-18°c), so far, coldadaption molecular mechanism of the bacteria is unknown. in this study, v. anguillarum was found possessing a putative glycine betaine ... | 2017 | 28035596 |
| leucine-rich repeats containing protein functions in the antibacterial immune reaction in stomach of kuruma shrimp marsupenaeus japonicus. | leucine rich repeat (lrr) motif exists in many immune receptors of animals and plants. most lrr containing (lrrc) proteins are involved in protein-ligand and protein-protein interaction, but the exact functions of most lrrc proteins were not well-studied. in this study, an lrrc protein was identified from kuruma shrimp marsupenaeus japonicus, and named as mjlrrc1. mjlrrc1 was consistently expressed in different tissues of normal shrimp with higher expression in gills and stomach. at the transcri ... | 2017 | 28027987 |
| development of a colloidal gold immunochromatographic strip for rapid detection of streptococcus agalactiae in tilapia. | a colloidal gold immunochromatographic strip was developed for rapid detection of streptococcus agalactiae (s. agalactiae) infection in tilapia. the monoclonal antibodies (mab) 4c12 and 3a9 were used to target s. agalactiae as colloidal gold-mab conjugate and captured antibody, respectively. the colloidal gold immunochromatographic strip was assembled via routine procedures. optimal ph and minimum antibody levels in the reaction system for gold colloidal-mab 4c12 conjugation were ph 7.4 and 18μg ... | 2017 | 27992801 |
| characterization of the traf3ip1 gene in yesso scallop (patinopecten yessoensis) and its expression in response to bacterial challenge. | tumor necrosis factor receptor-associated factor 3 (traf3) is an important adaptor that transmits upstream activation signals to induce innate immune responses. traf3 interacting protein 1 (traf3ip1) interacts specifically with traf3, but its function in innate immunity remains unclear, especially in marine invertebrates. in this study, to better understand the functions of trafs in innate immune responses, we identified and characterized the first bivalve traf3ip1 gene, pytraf3ip1, from yesso s ... | 2017 | 27990012 |
| changes in hemolymph characteristics of ark shell scapharaca broughtonii dealt with vibrio anguillarum challenge in vivo and various of anticoagulants in vitro. | the ark shell scapharca broughtonii is a commercially important shellfish in china. alserver's solution (as), modified alserver's solution (mas) and heparin sodium solution (hss) are common anticoagulants used for shellfish blood. to observe the immune response mediated by its hemocytes, we challenged in vivo s. broughtonii hemolymph with vibrio anguillarum and dealt with the following three anticoagulants in vitro: alserver's solution (as), modified alserver's solution (mas) and heparin sodium ... | 2017 | 27845210 |
| interaction of a 22 kda peptidyl prolyl cis/trans isomerase with the heat shock protein dnak in vibrio anguillarum. | peptidyl prolyl cis/trans isomerases (ppiases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds preceding prolines. we investigated the protein-protein interaction between a 22 kda ppiase (vafkbp22, an fk506-binding protein) and the molecular chaperone dnak derived from vibrio anguillarum o1 (vadnak) using gst pull-down assays and a bacterial two-hybrid system for in vivo and in vitro studies, respectively. furthermore, we analyzed the three-dimensional structure of the prot ... | 2017 | 27840395 |
| identification and functional characterizations of serpin8, a potential prophenoloxidase-activating protease inhibitor in pacific white shrimp, litopenaeus vannamei. | serpins have been characterized from varieties of organisms by their inhibitory roles on serine or cysteine proteases. however, research for the functional study of serpins in crustacean is relatively small. to fully clarify the immune characterizations of serpin, a novel serpin (named lvserpin8) encoding 414 amino acids with a 19-amino acid signal peptide and a serpin domain was identified from the pacific white shrimp litopenaeus vannamei. sequence analysis indicated that the genomic lvserpin8 ... | 2017 | 27836723 |
| the expression signatures of neuronal nitric oxide synthase (nos1) in turbot (scophthalmus maximus l.) mucosal surfaces against bacterial challenge. | the mucosal surfaces constitute the first immune barrier of host defense and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes. it has been long hypothesized that observed difference of disease resistance among different fish strains and species are strongly correlated to the activities of the immune actors in mucosal surfaces. particularly, neuronal nos (nnos or nos1) is a constitutively expressed gene that catalyzes the oxidati ... | 2016 | 27825948 |
| draft genome sequence of vibrio (listonella) anguillarum atcc 14181. | we report the draft genome sequence of vibrio anguillarum atcc 14181, a gram-negative, hemolytic, o2 serotype marine bacterium that causes mortality in mariculture species. the availability of this genome sequence will add to our knowledge of diversity and virulence mechanisms of vibrio anguillarum as well as other pathogenic vibrio spp. | 2016 | 27795288 |
| protective yeasts control v. anguillarum pathogenicity and modulate the innate immune response of challenged zebrafish (danio rerio) larvae. | we investigated mechanisms involved in the protection of zebrafish (danio rerio) larvae by two probiotic candidate yeasts, debaryomyces hansenii 97 (dh97) and yarrowia lypolitica 242 (yl242), against a vibrio anguillarum challenge. we determined the effect of different yeast concentrations (10(4)-10(7) cfu/ml) to: (i) protect larvae from the challenge, (ii) reduce the in vivo pathogen concentration and (iii) modulate the innate immune response of the host. to evaluate the role of zebrafish micro ... | 2016 | 27790411 |
| β-arrestin 1's interaction with tc45 attenuates stat signaling by dephosphorylating stat to inhibit antimicrobial peptide expression. | impaired phosphatase activity leads to the persistent activation of signal transducers and activators of transcription (stat). in mammals, stat family members are often phosphorylated or dephosphorylated by the same enzymes. to date, only one stat similar to mammalian stat5a/b has been found in crustaceans and there have been few studies in stat signal regulation in crustaceans. here, we report that β-arrestin1 interacts with tc45 (45-kda form of t cell protein tyrosine phosphatase) in the nucle ... | 2016 | 27782165 |
| molecular cloning, characterization and functional analysis of a heat shock protein 70 gene in cyclina sinensis. | heat shock protein 70 (hsp70) is an important member of the heat shock protein superfamily and is involved in protecting organisms against various stressors. in the present study, we used race to clone a full-length cyclina sinensis hsp70 cdna termed cshsp70. the full length of the cshsp70 cdna was 2308 bp, with a 5' untranslated region (utr) of 42 bp, a 3' utr of 268 bp, and an open reading frame (orf) of 1998 bp encoding a polypeptide of 655 amino acids with an estimated molecular mass of 72.7 ... | 2016 | 27725261 |
| molecular characterization, expression analysis, and bactericidal activity of the derivative peptides of tfpi-1 and tfpi-2 in half-smooth tongue sole, cynoglossus semilaevis. | tissue factor pathway inhibitors (tfpis) are kunitz-type serine protease inhibitors that reversibly regulate the blood coagulation induced by tissue factor. tfpi family contain two members, tfpi-1 and tfpi-2. recent studies have shown tfpi-1 and tfpi-2 also play important roles in innate immunity, however, the potential function of teleost tfpi are very limited. in this study, we characterized two tfpi (cstfpi-1 and cstfpi-2) molecules from half-smooth tongue sole (cynoglossus semilaevis), exami ... | 2016 | 27717901 |
| immunological function and antibacterial activity of two ferritin proteins from the freshwater pearl mussel hyriopsis schlegelii. | ferritin is a conserved iron-binding protein involved in host defense and cellular iron metabolism in most organisms. we investigated the expression profiles of two ferritin genes (designated hsfer-1 and hsfer-2) in the hemocytes, gonad, and hepatopancreas of hyriopsis schlegelii, when challenged with bacteria and metal ions. hsfer gene transcription increased 1.8-7.7- and 1.9-6.1-fold in these tissues after stimulation with staphylococcus aureus and vibrio anguillarum, respectively. in addition ... | 2016 | 27706646 |
| polymorphism in a serine protease inhibitor gene and its association with the resistance of bay scallop (argopecten irradians) to listonella anguillarum challenge. | serine protease inhibitors (spis) play a crucial role in regulation of both host and bacterial serine protease. they are classified into several protein families, where kazal-type inhibitors are one of families with multi-domain. in the present study, the polymorphism of aispi from bay scallop argopecten irradians was found to be associated with disease resistance of bay scallop against listonella anguillarum. nine single nucleotide polymorphisms (snps) were identified in the exon region of aisp ... | 2016 | 27697559 |
| production of the bioactive compounds violacein and indolmycin is conditional in a maea mutant of pseudoalteromonas luteoviolacea s4054 lacking the malic enzyme. | it has previously been reported that some strains of the marine bacterium pseudoalteromonas luteoviolacea produce the purple bioactive pigment violacein as well as the antibiotic compound indolmycin, hitherto only found in streptomyces. the purpose of the present study was to determine the relative role of each of these two compounds as antibacterial compounds in p. luteoviolacea s4054. using tn10 transposon mutagenesis, a mutant strain that was significantly reduced in violacein production in m ... | 2016 | 27695447 |
| the inhibition and resistance mechanisms of actinonin, isolated from marine streptomyces sp. nhf165, against vibrio anguillarum. | vibrio sp. is the most serious pathogen in marine aquaculture, and the development of anti-vibrio agents is urgently needed. however, it is extreme lack of high-throughput screening (hts) model for searching anti-vibrio compounds. here, we established a protein-based hts screening model to identify agents targeting peptide deformylase (pdf) of vibrio anguillarum. to find potential anti-vibrio compounds, crude extracts derived from marine actinomycetes were applied for screening with this model. ... | 2016 | 27679625 |
| identification and characterization of two croquemort homologues in penaeid shrimp litopenaeus vannamei. | croquemort, the homologue of human cd36, is a member of class b scavenger receptors, which is involved in bacteria phagocytosis and cytokins release. however, there is still less information about croquemort in crustaceans. here, a croquemort from pacific white shrimp litopenaeus vannamei (lvcroquemort) and its truncated form (lvcroquemort-s1) cdna sequences were identified, characterized and their role in bacteria clearance was investigated. the deduced protein of lvcroquemort is 533 amino acid ... | 2017 | 27670083 |
| mir-122 involved in the regulation of toll-like receptor signaling pathway after vibrio anguillarum infection by targeting tlr14 in miiuy croaker. | innate and acquired immune responses provide critical line in defense against pathogens. micrornas (mirnas) play crucial roles in regulate gene expression in inflammation and innate immunity. however, the main role of mirnas are in a large part unclear in aquatic organisms. in this study, we found that mir-122 displayed dramatically declined expression profiles in vibrio anguillarum challenged miiuy croaker. meanwhile, we have explored tlr14 as a novel target gene of mir-122 involved in miiuy cr ... | 2016 | 27637732 |
| identification of a new calreticulin homolog from yesso scallop (patinopecten yessoensis) and its role in innate immunity. | calreticulin (crt) is a multifunctional and highly conserved ca(2+)-binding protein shared among vertebrates and invertebrates. in this study, we cloned and characterized a crt gene, pycrt, from yesso scallop, patinopecten yessoensis. the full-length cdna of pycrt was 1830 bp, including a 1242 bp open reading frame (orf), a 29 bp 5'-untranslated region and a 559 bp 3'-untranslated region. pycrt was consisted of three distinct structural and functional domains (n-, p- and c-domains), a signal pep ... | 2016 | 27633681 |