Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| nosocomial outbreak of pantoea agglomerans bacteraemia associated with contaminated anticoagulant citrate dextrose solution: new name, old bug? | we describe an outbreak investigation of pantoea agglomerans bacteraemia associated with anticoagulant citrate-dextrose 46% (acd) solution prepared in-house. a healthy man presented with septic shock during plasmapheresis for granulocyte donation. the solution used for priming and blood samples were sent for culture. identification of the isolate to species level was performed by gyrb sequencing. typing was performed by pulsed-field gel electrophoresis (pfge). in total, eight cases were identifi ... | 2012 | 22245117 |
| isolation of endophytic bacteria from arboreal species of the amazon and identification by sequencing of the 16s rrna encoding gene. | endophytic bacteria from three arboreal species native to the amazon (carapa guianenses, ceiba pentandra, and swietenia macrophylla), were isolated and identified, through partial sequencing of the 16s rrna encoding gene. from these, 16 isolates were obtained, although, when compared to sequences deposited in genbank, only seven had produced identifiable fragments. bacillus, pantoea and two non-culturable samples were identified. results obtained through sequence analysis revealed low genetic di ... | 2011 | 22215973 |
| Complete Genome Sequence of the Rice Pathogen Pantoea ananatis Strain PA13. | Pantoea ananatis is the causative agent of sheath and grain rot in rice. Here, we present the complete genome sequence of P. ananatis strain PA13, originally isolated from a diseased rice grain. | 2012 | 22207741 |
| Characterization and screening of plant probiotic traits of bacteria isolated from rice seeds cultivated in Argentina. | Many seeds carry endophytes, which ensure good chances of seedling colonization. In this work, we have studied the seed-borne bacterial flora of rice varieties cultivated in the northeast of Argentina. Surface-sterilized husked seeds of the rice cultivars CT6919, El Paso 144, CAMBA, and IRGA 417 contained an average of 5×10(6) CFU/g of mesophilic and copiotrophic bacteria. Microbiological, physiological, and molecular characterization of a set of 39 fast-growing isolates from the CT6919 seeds re ... | 2011 | 22203552 |
| multiple origins of endosymbiosis within the enterobacteriaceae (gamma-proteobacteria): convergence of complex phylogenetic approaches. | abstract: background: the bacterial family enterobacteriaceae gave rise to a variety of symbiotic forms, from the loosely associated commensals, often designated as secondary (s) symbionts, to obligate mutualists, called primary (p) symbionts. determination of the evolutionary processes behind this phenomenon has long been hampered by the unreliability of phylogenetic reconstructions within this group of bacteria. the main reasons have been the absence of sufficient data, the highly derived nat ... | 2011 | 22201529 |
| Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8. | The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while ... | 2011 | 22187082 |
| Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria. | The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ... | 2011 | 22163022 |
| The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential. | Pantoea ananatis AJ13355 is a newly identified member of the Enterobacteriaceae family with promising biotechnological applications. This bacterium is able to grow at an acidic pH and is resistant to saturating concentrations of L-glutamic acid, making this organism a suitable host for the production of L-glutamate. In the current study, the complete genomic sequence of P. ananatis AJ13355 was determined. The genome was found to consist of a single circular chromosome consisting of 4,555,536 bp ... | 2011 | 22159605 |
| Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage. | Aims: Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. Methods and Results: Direct-cut and wilted guinea grass silage was prepared with and without molasses. A high acetic acid level was observed during the fermentation of direct-cut silage, and long storage inc ... | 2011 | 22150390 |
| high isoprenoid flux escherichia coli as a host for carotenoids production. | a noncarotenogenic microbe e. coli was engineered for high production of carotenoids. to increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (dxs, idi and ispdispf) in the isoprenoid pathway were replaced with a strong bacteriophage t5 promoter (p(t5)) by using the λ-red recombinase system in combination with the flp/frt site-specific recombination system for marker excision and p1 transduction for gene trait stacking. the resulting high isoprenoid flux e ... | 2012 | 22144352 |
| spm43.1 contributes to acid-resistance of non-symplasmata-forming cells in pantoea agglomerans ys19. | pantoea agglomerans ys19 is a rice endophytic bacterium characterized to form multicellular biofilm-like structures called symplasmata. phenotypic distinctions between symplasmata-forming cells and planktonic cells are crucial for understanding ys19's survival strategies. in this study, a 43.1 kda protein spm43.1 was identified to show significant resistance to the aggregation effect caused by denaturing acidic conditions. maldi-tof analysis data indicated that it is a maltose-binding protein ho ... | 2011 | 22134847 |
| pantoea stewartii subsp. stewartii produces an endoglucanase that is required for full virulence in sweet corn. | pantoea stewartii subsp. stewartii (pnss), a xylem-dwelling bacterium, is the causal agent of stewart's wilt and blight of sweet corn. the goal of this study was to characterize the only gene in the pnss genome predicted to encode an endoglucanase; this gene was designated engy. the recombinant engy protein and culture supernatants of pnss possessed both egase and xylanase activities. deletion of engy abolished egase and xylanase activity demonstrating that engy appears to be the major egase/x ... | 2011 | 22122328 |
| Comparative genomics of the type VI secretion systems of Pantoea and Erwinia species reveals the presence of putative effector islands that may be translocated by the VgrG and Hcp proteins. | ABSTRACT: | 2011 | 22115407 |
| The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation. | Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologous operons that have been partially characterized, although some annotations are solely on the basis of sequence homology. The major distinguishing features of these two EPS forms are the presence of a terminal pyr ... | 2012 | 22111898 |
| antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria). | the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ... | 2011 | 22095134 |
| Pantoea clinical isolates cannot be accurately assigned to species based on metabolic profiling. | 2011 | 22093950 | |
| dinitrogen fixation of microbe-plant associations as affected by nitrate and ammonium supply. | abstract the dinitrogen fixation activity of azospirillum sp., and pantoea agglomerans strains was determined by (15)n(2) incorporation after incubation with (15)n(2) labeled air or/and by acetylene reduction. these bacterial strains were able to fix n(2) both in pure culture and in association with wheat plants in hydroponics. nitrogenase activity of azospirillum sp., in pure culture was more rapidly inhibited by the addition of nh(4) (+) than no(3) (-). the n(2) fixation of p. agglomerans de ... | 1997 | 22087483 |
| cow teat skin, a potential source of diverse microbial populations for cheese production. | the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ... | 2012 | 22081572 |
| culturable bacterial communities on leaf sheaths and panicles of rice plants in japan. | culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ... | 2011 | 22081407 |
| E622, a miniature, virulence-associated mobile element. | Miniature inverted terminal repeat elements (MITEs) are nonautonomous mobile elements that have a significant impact on bacterial evolution. Here we characterize E622, a 611-bp virulence-associated MITE from Pseudomonas syringae, which contains no coding region but has almost perfect 168-bp inverted repeats. Using an antibiotic coupling assay, we show that E622 is transposable and can mobilize an antibiotic resistance gene contained between its borders. Its predicted parent element, designated T ... | 2012 | 22081398 |
| characterization of phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants. | this study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. results showed that bacterial treatment followed by wetland plants (phragmites cummunis) resulted 94.5% and 96.0% reduction in bod and cod values, respectively. the pcr-rflp analysis showed the presence of stenotrophomonas, enterobacter, pantoea, acinetobacter an ... | 2012 | 22047662 |
| massive production of butanediol during plant infection by phytopathogenic bacteria of the genera dickeya and pectobacterium. | plant pathogenic bacteria of the genera dickeya and pectobacterium are broad-host-range necrotrophs which cause soft-rot diseases in important crops. a metabolomic analysis, based on (13)c-nmr spectroscopy, was used to characterize the plant-bacteria interaction. metabolic profiles revealed a decline in plant sugars and amino acids during infection and the concomitant appearance of a compound identified as 2,3-butanediol. butanediol is the major metabolite found in macerated tissues of various h ... | 2011 | 22032684 |
| screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40. | the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated. | 2012 | 22017666 |
| Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora. | Stenocarpella maydis and Stenocarpella macrospora are the causal agents of ear rot in corn, which is one of the most destructive diseases in this crop worldwide. These fungi are important mycotoxin producers that cause different pathologies in farmed animals and represent an important risk for humans. In this work, 160 strains were isolated from soil of corn crops of which 10 showed antifungal activity against these phytopathogens, which, were identified as: Bacillus subtilis, Pseudomonas spp., ... | 2011 | 22016606 |
| Bacterial skin flora variation and in vitro inhibitory activity against Saprolegnia parasitica in brown and rainbow trout. | Variations in the number and diversity of bacteria from the skin of brown trout Salmo trutta L. and rainbow trout Oncorhynchus mykiss Walbaum were surveyed from different rivers and fish farms in northern Spain. In addition to determining bacterial populations in skin samples of healthy fish, bacterial populations were determined from skin lesions (of brown trout only) infected with Saprolegnia parasitica, the causal agent of saprolegniosis. Mean bacterial counts from skin lesions of brown trout ... | 2011 | 22013752 |
| The Type III Effector HsvG of the Gall-Forming Pantoea agglomerans Mediates Expression of the Host Gene HSVGT. | The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae (Pag) is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein, but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila ... | 2011 | 21995766 |
| probing the impact of ligand binding on the acyl-homoserine lactone-hindered transcription factor esar of pantoea stewartii subsp. stewartii. | the quorum-sensing regulator esar from pantoea stewartii subsp. stewartii is a luxr homologue that is inactivated by acyl-homoserine lactone (ahl). in the corn pathogen p. stewartii, production of exopolysaccharide (eps) is repressed by esar at low cell densities. however, at high cell densities when high concentrations of its cognate ahl signal are present, esar is inactivated and derepression of eps production occurs. thus, esar responds to ahl in a manner opposite to that of most luxr family ... | 2011 | 21949066 |
| Emergence of blaCTX-M-15, qnrB1 and aac(6')-Ib-cr resistance genes in Pantoea agglomerans and Enterobacter cloacae from Nigeria (sub-Saharan Africa). | 2012 | 21921107 | |
| efficient synthesis of hydroxystyrenes via biocatalytic decarboxylation/deacetylation of substituted cinnamic acids by newly isolated pantoea agglomerans strains. | background: decarboxylation of substituted cinnamic acids is a predominantly followed pathway for obtaining hydroxystyrenes-one of the most extensively explored bioactive compounds in the food and flavor industry (e.g. fema gras approved 4-vinylguaiacol). for this, mild and green strategies providing good yields with high product selectivity are needed. results: two newly isolated bacterial strains, i.e. pantoea agglomerans kjlpb4 and p. agglomerans kjpb2, are reported for mild and effective dec ... | 2011 | 21919002 |
| vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital. | an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ... | 2011 | 21917010 |
| Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis SK-1. | Pantoea ananatis SK-1 produces N-acylhomoserine lactones (AHLs) and regulates expression of some virulence factors through AHL-mediated quorum sensing. In this study, we discovered that the strain SK-02I, which has a disrupted AHL-synthetic gene, showed constitutive cell aggregation. SK-1 has the ability to aggregate, and cell aggregation inhibitory factors are expressed under control of AHL-mediated quorum sensing. One of the transposon mutants, SK-33M, constitutively aggregates without defecti ... | 2011 | 21900041 |
| Proposal to reclassify Brenneria quercina (Hildebrand & Schroth 1967) Hauben et al. 1999 into a novel genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii. | Bacterial isolates from oak in Spain and Britain, showing symptoms of bark canker and Acute Oak Decline (AOD) respectively, were examined by a polyphasic approach. Both 16S rRNA gene sequencing and multilocus sequence analysis (MLSA), based on partial sequences of gyrB, rpoB, infB and atpD, revealed that the isolates were separated into two genetic groups according to their origin. Their closest phylogenetic relative was B. quercina, the causal agent of drippy nut disease of oak, which clustered ... | 2011 | 21890733 |
| Functional characterization of lipopolysaccharide derived from symbiotic bacteria in rice as a macrophage-activating substance. | Lipopolysaccharide derived from a symbiotic bacterium in wheat (Pantoea agglomerans, LPSp) has shown multiple positive effects, such as prophylactic, antiallergic and antitumour effects, without serious side-effects. LPSp has differential biological activities in comparison to other LPS, such as those from Escherichia coli (LPSe). The only difference between LPSp and LPSe is in the O-antigen polysaccharide structure (O-PS). This led us to the hypothesis that the O-PS structure would seem to part ... | 2011 | 21873161 |
| lipopolysaccharide ip-pa1 from pantoea agglomerans prevents suppression of macrophage function in stress-induced diseases. | chronic psychological stress impairs health and induces various diseases by causing an imbalance in the immune, neuropsychiatric and endocrine systems. the primary reason for the development of stress-induced disease is suppression of macrophage function, which plays a pivotal role in innate immunity. in fact, surgical stress has been shown to exacerbate opportunistic infections by significantly suppressing macrophage function. conversely, administration of macrophage activating substances befor ... | 2011 | 21873156 |
| Gamma-radiation induced modifications in substrate specificity of glucose dehydrogenase and carbon source utilization pattern of phosphate-solubilizing Pantoea strains. | Glucose, maltose, and mannose as sole carbon sources, induced synthesis of glucose dehydrogenase (GDH) in three strains of Pantoea with specific activities from 0.14 to 0.6 U/mg proteins. Utilization of lactose indicated that the enzyme belongs to GDH type B isozyme. Of mutant clones, developed through radiation mutagenesis, P2-M2 utilized ribose with GDH specific activity of 0.57 U/mg protein, P4-M3 grown on glucose gave 1.5 U/mg protein and P4-M5 had high activities, when grown on galactose, m ... | 2011 | 21866443 |
| hessian fly-associated bacteria: transmission, essentiality, and composition. | plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ... | 2011 | 21858016 |
| pantoea rodasii sp. nov., pantoea rwandensis sp. nov. and pantoea wallisii sp. nov., three novel pantoea species isolated from eucalyptus. | several gram-negative, facultatively anaerobic bacterial isolates were obtained from eucalyptus seedlings showing symptoms of bacterial blight and dieback in colombia, rwanda and south africa. partial 16s rrna gene sequencing, together with partial gyrb sequencing, placed the isolates in the genus pantoea and indicated they constituted three novel species. multilocus sequence analysis (mlsa) based on partial sequences of gyrb, rpob, infb and atpd revealed p. dispersa, p. eucrina and p. cypripedi ... | 2011 | 21841003 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| genomics of iron acquisition in the plant pathogen erwinia amylovora: insights in the biosynthetic pathway of the siderophore desferrioxamine e. | genomics has clarified the biosynthetic pathway for desferrioxamine e critical for iron acquisition in the enterobacterial fire blight pathogen erwinia amylovora. evidence for each of the individual steps and the role of desferrioxamine e biosynthesis in pathogen virulence and cell protection from host defenses is presented. using comparative genomics, it can be concluded that desferrioxamine biosynthesis is ancestral within the genera erwinia and pantoea. | 2011 | 21814817 |
| molecular and physiological properties of bacteriophages from north america and germany affecting the fire blight pathogen erwinia amylovora. | for possible control of fire blight affecting apple and pear trees, we characterized erwinia amylovora phages from north america and germany. the genome size determined by electron microscopy (em) was confirmed by sequence data and major coat proteins were identified from gel bands by mass spectroscopy. by their morphology from em data, fea1h and fea100 were assigned to the podoviridae and fea104 and fea116 to the myoviridae. host ranges were essentially confined to e. amylovora, strains of the ... | 2011 | 21791029 |
| metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist pantoea vagans c9-1. | pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by erwinia amylovora. compared to other biocontrol agents, relatively little is currently known regarding pantoea genetics. better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy. | 2011 | 21789243 |
| anti-lipopolysaccharide factor evokes indirect killing of virulent bacteria in kuruma prawn. | aims: anti-lipopolysaccharide factor (alf) is an antimicrobial peptide (amp) and a key effector molecule of the innate immune system in crustaceans. however, little is known about the role of its indirect killing against bacteria. the possible regulatory role of this peptide (m-alf) in kuruma prawns, marsupenaeus japonicus, was investigated. | 2011 | 21753127 |
| mouse model of hypersensitivity pneumonitis after inhalation exposure to different microbial antigens associated with organic dusts. | the aim of this study was to reproduce in laboratory conditions hypersensitivity pneumonitis (hp) pathogenesis in a new animal model predictive of the human response, and to select the microbial antigen associated with organic dust that exerts the strongest pathogenic effect on the respiratory organ. to achieve this goal, mice of the strain c57bl/6j prone to fibrosis were exposed for 1 hour daily up to 28 days to the inhalation of aerosols of each of the 5 microbial components of organic dusts w ... | 2011 | 21736281 |
| exposure to bioaerosols in the selected agricultural facilities of the ukraine and poland - a review. | the aim of this work was to review the studies on bioaerosols which were carried out in the years 1972-2009 in following branches of agricultural industry in the ukraine and poland: animal farms for cows and pigs, animal feed facilities, production of biofuel from rape, herb farms and herb processing facilities. in all facilities were determined: concentration of dust and microorganisms in the air and species composition of microflora. moreover, in polish animal farms, herb farms and herb proces ... | 2011 | 21736265 |
| pantoea stewartii subsp. stewartii: lessons learned from a xylem-dwelling pathogen of sweet corn. | pantoea stewartii subsp. stewartii is a gram-negative enteric bacterium that primarily infects sweet corn. studies of this bacterium have provided useful insight into how xylem-dwelling bacteria establish themselves and incite disease in their hosts. pantoea stewartii subsp. stewartii is a remarkable bacterial system for laboratory studies because of its relative ease of propagation and genetic manipulation, and the fact that it appears to employ a minimal number of pathogenicity mechanisms. in ... | 2011 | 21726365 |
| antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket). | the aim of this study was to elucidate whether and to what extent fresh produce from germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. for this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in germany and examined bacteriologically. phenotypic resistance of enterobacter cloacae (n=172); enterobacter gergoviae (n=92); pantoea agglomerans (n=96); pseudomonas aeruginosa (n=295); pseudom ... | 2011 | 21700353 |
| bacterial community composition of biological degreasing systems and health risk assessment for workers. | biological degreasing system is a new technology based on the degradation capabilities of microorganisms to remove oil, grease, or lubricants from metal parts. no data is available about the potential biological health hazards in such system. thus, a health risk assessment linked to the bacterial populations present in this new degreasing technology is, therefore, necessary for workers. we performed both cultural and molecular approaches in several biological degreasing systems for various indus ... | 2011 | 21698403 |
| impact of derepressed ampc {beta}-lactamase act-9 on the clinical efficacy of ertapenem. | an in vivo development of pantoea agglomerans mutants (isolates pa2 to pa4) with reduced ertapenem susceptibility from that of isolate pa1 was associated with an inadequate clinical response to ertapenem therapy. all four isolates harbored the bla(act-9) ampc +¦-lactamase gene. however, a loss-of-function mutation in the ampd gene in pa2 to pa4, but not pa1, led to derepressed act-9. the reduced ertapenem susceptibility caused by derepressed act-9 was confirmed with an ampd knockout mutant of pa ... | 2011 | 21690276 |
| antibiosis activity of pantoea agglomerans biocontrol strain e325 against erwinia amylovora on apple flower stigmas. | pantoea agglomerans e325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a unique alkaline- and phosphate-sensitive antibiotic specific to erwinia amylovora. antibiosis was evaluated as a mode of antagonism on flower stigmas using two antibiosis-deficient mutants. on king's medium b, mutants e325ad1 and e325ad2 have stable smooth-butyrous or hypermucoid colony morphologies, respectively, and the parental strain e325 exhibits phenot ... | 2011 | 21679036 |
| Sharing of quorum-sensing signals and role of interspecies communities in a bacterial plant disease. | Pathogenic bacteria interact not only with the host organism but most probably also with the resident microbial flora. In the knot disease of the olive tree (Olea europaea), the causative agent is the bacterium Pseudomonas savastanoi pv. savastanoi (Psv). Two bacterial species, namely Pantoea agglomerans and Erwinia toletana, which are not pathogenic and are olive plant epiphytes and endophytes, have been found very often to be associated with the olive knot. We identified the chemical signals t ... | 2011 | 21677694 |
| conservation and diversity of seed associated endophytes in zea across boundaries of evolution, ethnography and ecology. | endophytes are non-pathogenic microbes living inside plants. we asked whether endophytic species were conserved in the agriculturally important plant genus zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from mexico to canada. kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and dna fingerprinting using terminal restriction fragment length polymorphis ... | 2011 | 21673982 |
| diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis. | microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ... | 2011 | 21660545 |
| rhizosphere competent pantoea agglomerans enhances maize (zea mays) and chickpea (cicer arietinum l.) growth, without altering the rhizosphere functional diversity. | plant growth promoting pantoea agglomerans nbrisrm (nbrisrm) was able to produce 60.4 μg/ml indole acetic acid and solubilize 77.5 μg/ml tri-calcium phosphate under in vitro conditions. addition of 2% nacl (w/v) in the media induced the iaa production and phosphate solubilization by 11% and 7%, respectively. for evaluating the plant growth promotory effect of nbrisrm inoculation a micro plot trial was conducted using maize and chickpea as host plants. the results revealed significant increase in ... | 2011 | 21638110 |
| characterization of a small cryptic plasmid from endophytic pantoea agglomerans and its use in the construction of an expression vector. | a circular cryptic plasmid named ppaga (2,734 bp) was isolated from pantoea agglomerans strain ege6 (an endophytic bacterial isolate from eucalyptus). sequence analysis revealed that the plasmid has a g+c content of 51% and contains four potential orfs, 238(a), 250(b), 131(c), and 129(d) amino acids in length without homology to known proteins. the shuttle vector plgm1 was constructed by combining the ppaga plasmid with pgfpmut3.0 (which harbors a gene encoding green fluorescent protein, gfp), a ... | 2011 | 21637551 |
| directed evolution of the nonribosomal peptide synthetase admk generates new andrimid derivatives in vivo. | many lead compounds in the search for new drugs derive from peptides and polyketides whose similar biosynthetic enzymes have been difficult to engineer for production of new derivatives. problems with generating multiple analogs in a single experiment along with lack of high-throughput methods for structure-based screening have slowed progress in this area. here, we use directed evolution and a multiplexed assay to screen a library of >14,000 members to generate three derivatives of the antibact ... | 2011 | 21609841 |
| secretion of anti-plasmodium effector proteins from a natural isolate pantoea agglomerans using pelb and hlya secretion signals. | the insect-vectored disease malaria is a major world health problem. new control strategies are needed to supplement the current use of insecticides and medications. a genetic approach can be used to inhibit development of the malaria parasite (plasmodium sp.) in the mosquito host. we hypothesized that pantoea agglomerans, a bacterial symbiont of anopheles mosquitoes, could be engineered to express and secrete anti-plasmodium effector proteins, a strategy termed paratransgenesis. to this end, pl ... | 2011 | 21602368 |
| exopolysaccharide production is influenced by sugars, n-acylhomoserine lactone, and transcriptional regulators rcsa and rcsb, but does not affect pathogenicity in the plant pathogen pantoea ananatis. | pantoea ananatis sk-1 produced eps by ahl-mediated quorum sensing on an lb agar plate containing glucose, fructose, and sucrose. rcsa and rcsb mutants did not produce eps with or without ahls and with or without sugars, but they induced necrotic symptoms in onion leaves. these results indicate that eps production does not relate to the pathogenicity of sk-1. | 2011 | 21597171 |
| population dynamics of bacteria associated with different strains of the pine wood nematode bursaphelenchus xylophilus after inoculation in maritime pine (pinus pinaster). | for a long time it was thought that bursaphelenchus xylophilus was the only agent of the pine wilt disease. recently, it was discovered that there are bacteria associated with the nematodes that contribute to the pathogenesis of this disease, mainly through the release of toxins that promote the death of the pines. among the species most commonly found, are bacteria belonging to the bacillus, pantoea, pseudomonas and xanthomonas genera. the main objective of this work was to study the effect of ... | 2011 | 21570967 |
| comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in ivory coast and brazil. | to compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one ivorian farm) and box (two brazilian farms) fermentations were carried out. all fermentations were studied through a multiphasic approach. in general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48°c. the main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mann ... | 2011 | 21569940 |
| rice seeds as sources of endophytic bacteria. | endophytic bacteria are considered to originate from the external environment. to examine the hypothesis that rice (oryza sativa, cultivar kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of the various bacterial strains isolated, the closest relatives, identified by 16s rrna gene sequencing, were: bacillus firmus, b. f ... | 2009 | 21566368 |
| stereochemistry and mechanism of a microbial phenylalanine aminomutase. | the stereochemistry of a phenylalanine aminomutase (pam) on the andrimid biosynthetic pathway in pantoea agglomerans (pa) is reported. papam is a member of the 4-methylidene-1h-imidazol-5(4h)-one (mio)-dependent family of catalysts and isomerizes (2s)-α-phenylalanine to (3s)-β-phenylalanine, which is the enantiomer of the product made by the mechanistically similar aminomutase tcpam from taxus plants. the nh(2) and pro-(3s) hydrogen groups at c(α) and c(β), respectively, of the substrate are rem ... | 2011 | 21561099 |
| a novel low-temperature-active β-glucosidase from symbiotic serratia sp. tn49 reveals four essential positions for substrate accommodation. | a 2,373-bp full-length gene (bgla49) encoding a 790-residue polypeptide (bgla49) with a calculated mass of 87.8 kda was cloned from serratia sp. tn49, a symbiotic bacterium isolated from the gut of longhorned beetle (batocera horsfieldi) larvae. the deduced amino acid sequence of bgla49 showed the highest identities of 80.1% with a conceptually translated protein from pantoea sp. at-9b (eew02556), 38.3% with the identified glycoside hydrolase (gh) family 3 β-glucosidase from clostridium stercora ... | 2011 | 21559826 |
| endophytic bacteria in the rice plant. | endophytic bacteria are defined as bacteria detected inside surface-sterilized plants or extracted from inside plants and having no visibly harmful effects on the plants. various kinds of endophytic bacteria, such as pantoea, methylobacterium, azospirillum, herbaspirillum, burkholderia and rhizobium etc., have been found inside rice plants. this minireview summarizes and discusses recent studies of endophytic bacteria residing in rice plants, focusing on flora, origin, movement, and interaction ... | 2008 | 21558696 |
| kinetics of bacterial potentiometric titrations: the effect of equilibration time on buffering capacity of pantoea agglomerans suspensions. | several recent studies have made use of continuous acid-base titration data to describe the surface chemistry of bacterial cells as a basis for accurately modelling metal adsorption to bacteria and other biomaterials of potential industrial importance. these studies do not share a common protocol; rather they titrate in different ph ranges and they use different stability criteria to define equilibration time during titration. in the present study we investigate the kinetics of bacterial titrati ... | 2011 | 21543082 |
| classification of plant associated bacteria using rif, a computationally derived dna marker. | a dna marker that distinguishes plant associated bacteria at the species level and below was derived by comparing six sequenced genomes of xanthomonas, a genus that contains many important phytopathogens. this dna marker comprises a portion of the dnaa replication initiation factor (rif). unlike the rrna genes, dnaa is a single copy gene in the vast majority of sequenced bacterial genomes, and amplification of rif requires genus-specific primers. in silico analysis revealed that rif has equal or ... | 2011 | 21533033 |
| engineering of a plasmid-free escherichia coli strain for improved in vivo biosynthesis of astaxanthin. | abstract: background: the xanthophyll astaxanthin is a high-value compound with applications in the nutraceutical, cosmetic, food, and animal feed industries. besides chemical synthesis and extraction from naturally producing organisms like haematococcus pluvialis, heterologous biosynthesis in non-carotenogenic microorganisms like escherichia coli, is a promising alternative for sustainable production of natural astaxanthin. recent achievements in the metabolic engineering of e. coli strains hav ... | 2011 | 21521516 |
| a head-to-head comparison of eneamide and epoxyamide inhibitors of glucosamine-6-phosphate synthase from the dapdiamide biosynthetic pathway. | the dapdiamides make up a family of antibiotics that have been presumed to be cleaved in the target cell to enzyme-inhibitory n-acyl-2,3-diaminopropionate (dap) warheads containing two alternative electrophilic moieties. our prior biosynthetic studies revealed that an eneamide warhead is made first and converted to an epoxyamide via a three-enzyme branch pathway. here we provide a rationale for this logic. we report that the r,r-epoxyamide warhead is a more efficient covalent inactivator of gluc ... | 2011 | 21520904 |
| oral administration of immunopotentiator from pantoea agglomerans 1 (ip-pa1) improves the survival of b16 melanoma-inoculated model mice. | to investigate the usefulness of the immunopotentiator from pantoea agglomerans 1 (ip-pa1) as a supportive drug in melanoma therapy, we analyzed the immunological effects of ip-pa1 on melanoma-inoculated model mice. oral administration of ip-pa1 increased the serum levels of tumor necrosis factor (tnf)-α at 2 h after the administration and interferon (ifn)-γ and il-12 at 12 h after the administration in naïve balb/ccrslc mice as evaluated by elisa. ip-pa1 did not affect the proliferation of mela ... | 2011 | 21512265 |
| bacterial community diversity and variation in spray water sources and the tomato fruit surface. | abstract: | 2011 | 21510867 |
| pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts. | a. shubov, p. jagannathan, p. v. chin-hong. pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts. transpl infect dis 2011. all rights reserved abstract: pantoea agglomerans is a gram-negative rod that is frequently found on the exterior of many plants, fruits, vegetables, and in soil, and it is used as a biopesticide in the agriculture industry. recent reports have implicated p. agglomerans in systemic inf ... | 2011 | 21504526 |
| changes in bacterial flora of japanese cabbage during growth and potential source of flora. | bacterial flora of cabbage were identified and enumerated during various stages of growth, and the potential sources of contamination in the field were determined. bacterial counts increased from below the level of detection (2.4 log cfu/g) on seeds to 2.5 to 5.7 log cfu/g on seedlings. after transplanting, the counts of mesophilic aerobic bacteria on leaves decreased and then increased to 5.7 log cfu/g on outer leaves, 5.0 log cfu/g on middle leaves, and 3.0 log cfu/g on inner leaves at the har ... | 2011 | 21477482 |
| unusual causes of peritonitis in a peritoneal dialysis patient: alcaligenes faecalis and pantoea agglomerans. | an 87 -year-old female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. with the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients. | 2011 | 21477370 |
| plant growth promotion traits of phosphobacteria isolated from puna, argentina. | the ability of soil microorganisms to solubilize phosphate is an important trait of plant growth-promoting bacteria leading to increased yields and smaller use of fertilizers. this study presents the isolation and characterization of phosphobacteria from puna, northwestern argentina and the ability to produce phosphate solubilization, alkaline phosphatase, siderophores, and indole acetic acid. the p-solubilizing activity was coincidental with a decrease in ph values of the tricalcium phosphate m ... | 2011 | 21442320 |
| improved resistance against botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production. | bacteria such as pantoea agglomerans (pa-af2), bacillus subtilis (bs-271), acinetobacter lwoffii (al-113), and pseudomonas fluorescens (pf-ct2), originating from the vineyard, can induce defense responses and enhance resistance of grapevine against the fungal pathogen botrytis cinerea. the perception of these bacteria by plant cells or tissues in relation to their activities remains unknown. in this study, we examined the relationships between the activity of each bacterium to induce or prime so ... | 2011 | 21425931 |
| bacteriophages limelight and limezero of pantoea agglomerans, belonging to the "phikmv-like viruses". | pantoea agglomerans is a common soil bacterium used in the biocontrol of fungi and bacteria but is also an opportunistic human pathogen. it has been described extensively in this context, but knowledge of bacteriophages infecting this species is limited. bacteriophages limezero and limelight of p. agglomerans are lytic phages, isolated from soil samples, belonging to the podoviridae and are the first pantoea phages of this family to be described. the double-stranded dna (dsdna) genomes (43,032 b ... | 2011 | 21421778 |
| spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. | the spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. the proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) and dna sequencing in order to relate them to the performance and stability of the bioelectrochemical system. the reproducibility o ... | 2011 | 21400098 |
| characterization of nuclear localization signals in the type iii effectors hsvg and hsvb of the gall-forming bacterium pantoea agglomerans. | hsvg and hsvb, two paralogous type iii effectors of the gall-forming bacteria pantoea agglomerans pv. gypsophilae and p. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. they were previously shown to be dna-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. sequence analysis of these effectors did not detect canonical nuclear localization signals (nlss), but two basic amino acid clusters designated putative ... | 2011 | 21372093 |
| cellulose-degrading bacteria associated with the invasive woodwasp sirex noctilio. | sirex noctilio is an invasive wood-feeding wasp that threatens the world's commercial and natural pine forests. successful tree colonization by this insect is contingent on the decline of host defenses and the ability to utilize the woody substrate as a source of energy. we explored its potential association with bacterial symbionts that may assist in nutrient acquisition via plant biomass deconstruction using growth assays, culture-dependent and -independent analysis of bacterial frequency of a ... | 2011 | 21368904 |
| dynamics and species diversity of communities of lactic acid bacteria and acetic acid bacteria during spontaneous cocoa bean fermentation in vessels. | to speed up research on the usefulness and selection of bacterial starter cultures for cocoa bean fermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions was developed successfully. therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20 kg scale were tried out in triplicate. the community dynamics and kinetics of these fermentations were studied through a multiphasic approach. microbiological analysis revealed a limited bacterial s ... | 2010 | 21356451 |
| optimization of medium for indole-3-acetic acid production using pantoea agglomerans strain pvm. | to optimize the medium components for the production of indole-3-acetic acid (iaa) by isolated bacterium pantoea agglomerans strain pvm. | 2011 | 21332896 |
| the effect of handwashing with water or soap on bacterial contamination of hands. | handwashing is thought to be effective for the prevention of transmission of diarrhoea pathogens. however it is not conclusive that handwashing with soap is more effective at reducing contamination with bacteria associated with diarrhoea than using water only. in this study 20 volunteers contaminated their hands deliberately by touching door handles and railings in public spaces. they were then allocated at random to (1) handwashing with water, (2) handwashing with non-antibacterial soap and (3) ... | 2011 | 21318017 |
| bacterial species associated with sound and botrytis-infected grapes from a greek vineyard. | grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ... | 2011 | 21315469 |
| characterization of acc deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of brassica napus. | one hundred cu-resistant-endophytic bacteria were isolated from cu-tolerant plants grown on cu mine wasteland, of which, eight cu-resistant and 1-aminocyclopropane-1-carboxylate (acc) deaminase-producing endophytic bacteria were obtained based on the acc deaminase activity of the bacteria and characterized with respect to metal resistance, production of acc deaminase, indole-3-acetic acid (iaa) as well as siderophores and mineral phosphate solubilization. ralstonia sp. j1-22-2, pantoea agglomera ... | 2011 | 21315404 |
| identification of pantoea ananatis gene encoding membrane pyrroloquinoline quinone (pqq)-dependent glucose dehydrogenase and pqqabcdef operon essential for pqq biosynthesis. | pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. computer analysis of the p. ananatis sc17(0) sequenced genome revealed an orf encoding a homologue (named gcd) of the mgdh (ec 1.1.99.17) apoenzyme from escherichia coli and a putative pyrroloquinoline quinone (pqq) biosynthetic operon homologous to pqqabcdef from klebsiella pneumoniae. construction of ?gcd and ?pqq mutants of p. ananatis confirmed the proposed functions of these genetic elements. the p. ana ... | 2011 | 21306430 |
| phosphate-solubilization mechanism and in vitro plant growth promotion activity mediated by pantoea eucalypti isolated from lotus tenuis rhizosphere in the salado river basin (argentina). | to isolate and characterize phosphate-solubilizing strains from a constrained environment such as the salado river basin and to assess their phosphate-solubilizing mechanisms, to further selection of the most promising strains to inoculate and improve the implantation and persistence of lotus tenuis in the most important area devoted to meat-cow production in argentina. | 2011 | 21299771 |
| [isolation, identification and characterization of acc deaminase-containing endophytic bacteria from halophyte suaeda salsa]. | we isolated and characterized 1-aminocyclopropane-1-carboxylate (acc) deaminase-containing endophytic bacteria from halophyte suaeda salsa to understand the interactions between endophytes and halophyte. | 2010 | 21268896 |
| differences in bacterial diversity of host-associated populations of phylloxera notabilis pergande (hemiptera: phylloxeridae) in pecan and water hickory. | host-associated differentiation (had) is the presence of genetically divergent, host-associated populations. it has been suggested that microbial symbionts of insect herbivores may play a role in had by allowing their insect hosts to use different plant species. the objective of this study was to document if host-associated populations of phylloxera notabilis pergande (hemiptera: phylloxeridae) in pecan and water hickory corresponded with differences in the composition of their associated bacter ... | 2011 | 21261774 |
| characterization of the rcsc sensor kinase from erwinia amylovora and other enterobacteria. | rcsc is a hybrid sensor kinase which contains a sensor domain, a histidine kinase domain, and a receiver domain. we have previously demonstrated that, although the erwinia amylovora rcsc mutant produces more amylovoran than the wild-type (wt) strain in vitro, the mutant remains nonpathogenic on both immature pear fruit and apple plants. in this study, we have comparatively characterized the erwinia rcsc and its homologs from various enterobacteria. results demonstrate that expression of the erwi ... | 2011 | 21261468 |
| enterobacterial microflora in infancy - a case study with enhanced enrichment. | to validate pmeu (portable microbe enrichment unit) method for monitoring the composition and development of infantile intestinal enterobacterial microflora. | 2011 | 21249478 |
| multiple treatment meta-analysis of products evaluated for control of fire blight in the eastern united states. | the aim of this analysis was to estimate the effect sizes and consistency of products evaluated for fire blight control in the eastern united states over the last decade. because only 3% of the 69 studies published from 2000 to 2008 explicitly presented a measure of within-study variability, a method for estimating the least significant difference (lsd) and, hence the sampling variance, for studies with at least two significant mean separations in the presented mean multiple comparisons was deve ... | 2011 | 21244224 |
| soil type affects plant colonization, activity and catabolic gene expression of inoculated bacterial strains during phytoremediation of diesel. | the combined use of plants and associated microorganisms has great potential for cleaning up soils contaminated with petroleum hydrocarbons. apart from environmental conditions the physicochemical properties of the soil are the main factors influencing the survival and activity of an inoculated strain as well as the growth of plants. this study examined the effect of different soil types (sandy, loamy sand and loam) on the survival, gene abundance and catabolic gene expression of two inoculated ... | 2010 | 21216097 |
| bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri. | to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage. | 2011 | 21204884 |
| erwinia oleae sp. nov., isolated from olive knots caused by pseudomonas savastanoi pv. savastanoi. | three endophytic bacterial isolates were obtained in italy from olive knots caused by pseudomonas savastanoi pv. savastanoi. phenotypic tests in combination with 16s rrna gene sequence analysis indicated a phylogenetic position of these isolates in the genus erwinia or pantoea, and revealed two other strains with highly similar 16s rrna gene sequences (> 99 %), cect 5262 and cect 5264, obtained in spain from olive knots. rep-pcr dna fingerprinting of the five strains from olive knots with box, e ... | 2010 | 21186287 |
| isolation and characterisation of bacterial colonies from seeds and in vitro cultures of fraxinus spp. from italian sites. | culturable bacteria were isolated from seeds, embryos and contaminated in vitro cultures of ash (fraxinus excelsior l., f. ornus l. and f. angustifolia l.) and were identified using morphological and molecular analyses. fourteen morphologically distinct isolates were recovered from seeds of fraxinus spp. 16s rdna sequencing categorised these isolates into ten separate genera. three strains isolated from contaminated in vitro cultures, pantoea agglomerans, staphylococcus succinus and aerococcus v ... | 2011 | 21143738 |
| paenibacillus polymyxa jb05-01-1 and its perspectives for food conservation and medical applications. | the aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. a novel strain, termed paenibacillus polymyxa jb05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16s rrna gene sequence. this strain was able to inhibit growth of gram-negative bacteria including escherichia coli rr1, pseudomonas fluores ... | 2010 | 21132276 |
| dynamics, diversity and function of endophytic siderophore-producing bacteria in rice. | siderophore production confers to bacteria competitive advantages to colonize plant tissues and to exclude other microorganisms from the same ecological niche. this work shows that the community of endophytic siderophore-producing bacteria (spb) associated to oryza sativa cultivated in uruguayan soils is dynamic and diverse. these bacteria were present in grains, roots, and leaves, and their density fluctuated between log(10) 3.44 and log(10) 5.52 cfu g(-1) fresh weight (fw) during the plant gro ... | 2010 | 21128071 |
| effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants. | rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ... | 2010 | 21124052 |
| a case of pantoea endophthalmitis. | a previously healthy 50-year-old man was transferred to our hospital for evaluation of acute inflammation in his right eye after ocular trauma while using a grass mower. slit lamp examination showed 1 mm-length full thickness corneal laceration without leakage, 4+ cells and inflammatory membrane in the anterior chamber, 10% hypopyon, posterior synechiae formation, and cataract change. upon orbital computerized tomography, a metallic intraocular foreign body in the lens was identified. vitrectomy ... | 2010 | 21052515 |
| the bacterial community in 'taberna' a traditional beverage of southern mexico. | to characterize the bacterial community of taberna, an alcoholic traditional beverage from the southern part of mexico produced by the fermentation of the coyol palm sap (acrocomia aculeate). | 2010 | 21039665 |
| biosynthesis of zeaxanthin in recombinant pseudomonas putida. | pseudomonas putida kt2440 strain was investigated for biosynthesis of the valuable xanthophyll zeaxanthin. a new plasmid was constructed harboring five carotenogenic genes from pantoea ananatis and three genes from escherichia coli under control of an l: -rhamnose-inducible promoter. pseudomonas putida kt2440 wild type hardly tolerated the plasmids for carotenoid production. mating experiments with e. coli s17-1 strains revealed that the carotenoid products are toxic to the pseudomonas putida ce ... | 2010 | 21038098 |