Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| cloning and expression analysis of a toll-like receptor 21 (tlr21) gene from turbot, scophthalmus maximus. | toll-like receptor 21 (tlr21) is a non-mammalian tlr recognizing unmethylated cpg dna and considered as a functional homolog of mammalian tlr9. in the present study, a tlr21 gene was cloned from turbot, scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. the turbot (sm)tlr21 gene is an intronless gene with a length of 3527 bp and encodes a peptide of 984 amino acids. the deduced protein possesses a signal peptide sequence, a leucine-rich repeat (lrr) domain c ... | 2017 | 28359672 |
| characterization and expression analysis of chitinase genes (chit1, chit2 and chit3) in turbot (scophthalmus maximus l.) following bacterial challenge. | chitinases are hydrolytic enzymes which have been employed to breakdown chitin coats of pathogenic microorganisms, thereby weaken the defense system of several pathogens and insects. in this regard, we identified the chitinase genes of turbot and characterized their expression patterns in mucosal tissues following vibrio anguillarum and streptococcus iniae challenge. in present study, transcripts of three chitinase genes (chit1, chit2 and chit3) were captured, as well as their protein structures ... | 2017 | 28286313 |
| comparative assessment of vibrio virulence in marine fish larvae. | vibrionaceae infections are a major obstacle for marine larviculture; however, little is known about virulence differences of vibrio strains. the virulence of vibrio strains, mostly isolated from vibriosis outbreaks in farmed fish, was tested in larval challenge trials with cod (gadus morhua), turbot (scophthalmus maximus) and halibut (hippoglossus hippoglossus) using a multiwell dish assays with single-egg/larvae cultures. the strains differed significantly in virulence as some caused a high mo ... | 2017 | 28160295 |
| molecular characterization and quantification of estrogen receptors in turbot (scophthalmus maximus). | estrogens regulate various reproductive processes via estrogen receptor (er)-mediated signaling pathway in vertebrates. in this study, full-length sequences coding for erα, erβ1 and erβ2 were isolated from female turbot (scophthalmus maximus) by homology cloning and a strategy based on rapid amplification of cdna end-polymerase chain reaction (race-pcr). the nucleotide and amino acid sequences of turbot ers showed high homologies with the corresponding sequences of other fish species and signifi ... | 2017 | 28087301 |
| integrating genomic resources of flatfish (pleuronectiformes) to boost aquaculture production. | flatfish have a high market acceptance thus representing a profitable aquaculture production. the main farmed species is the turbot (scophthalmus maximus) followed by japanese flounder (paralichthys olivaceous) and tongue sole (cynoglossus semilaevis), but other species like atlantic halibut (hippoglossus hippoglossus), senegalese sole (solea senegalensis) and common sole (solea solea) also register an important production and are very promising for farming. important genomic resources are avail ... | 2017 | 28063346 |
| genomic sequencing of ranaviruses isolated from turbot (scophthalmus maximus) and atlantic cod (gadus morhua). | ranaviruses have been isolated from atlantic cod (gadus morhua) and turbot (scophthalmus maximus) in denmark. phylogenomic analyses revealed that these two ranaviruses are nearly identical and form a distinct clade at the base of the ranavirus tree branching off near other fish ranaviruses. | 2016 | 27979944 |
| the expression signatures of neuronal nitric oxide synthase (nos1) in turbot (scophthalmus maximus l.) mucosal surfaces against bacterial challenge. | the mucosal surfaces constitute the first immune barrier of host defense and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes. it has been long hypothesized that observed difference of disease resistance among different fish strains and species are strongly correlated to the activities of the immune actors in mucosal surfaces. particularly, neuronal nos (nnos or nos1) is a constitutively expressed gene that catalyzes the oxidati ... | 2016 | 27825948 |
| pathogen-dependent role of turbot (scophthalmus maximus) interferon-gamma. | interferon-gamma has been typically described as a pro-inflammatory cytokine playing an important role in the resolution of both viral and bacterial diseases. nevertheless, some anti-inflammatory functions are also attributed to this molecule. in this work we have characterized for the first time the turbot (scophthalmus maximus) interferon-gamma gene (ifng) and its expression pattern under basal conditions, after type i ifns administration, and viral and bacterial infection. the intramuscular i ... | 2016 | 27742586 |
| identification and expression analysis of toll-like receptor genes (tlr8 and tlr9) in mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | mucosal immune system is one of the most important components in the innate immunity and constitutes the front line of host defense against infection, especially for teleost, which are living in the pathogen-rich aquatic environment. the pathogen recognition receptors (prrs), which can recognize the conserved pathogen-associated molecular patterns (pamps) of bacteria, are considered as one of the most important component for pathogen recognition and immune signaling pathways activation in mucosa ... | 2016 | 27633670 |
| immunological characterization and expression of lily-type lectin in response to environmental stress in turbot (scophthalmus maximus). | lectins are a superfamily of carbohydrate-binding proteins that are widely distributed throughout living organisms. in earlier work, we identified lily-type lectin (smltl) in the skin mucus of turbot scophthalmus maximus, and we characterized the protein in the present study. results from qrt-pcr indicated that smltl was expressed highly in skin, intestine and gill tissue. changes in smltl expression occurred in these tissues in response to environmental stressors including ciliate infection, hi ... | 2016 | 27542614 |
| pharmacokinetic model of florfenicol in turbot (scophthalmus maximus): establishment of optimal dosage and administration in medicated feed. | the pharmacokinetics of florfenicol (ff) in turbot (scophthalmus maximus) was studied after single intravenous (10 mg kg(-1) ) and oral (100 mg kg(-1) ) administration. the plasma concentration-time data of florfenicol were described by an open one-compartment model. the elimination half-life (t1/2 ) was estimated to be 21.0 h, and the total body clearance, cl, was determined as 0.028 l kg h(-1) . the apparent volume distribution (vd ) was calculated to be 0.86 l kg(-1) and the mean residence ti ... | 2017 | 27502011 |
| characterization and expression analysis of a peptidoglycan recognition protein gene, smpgrp2 in mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | peptidoglycan recognition receptor proteins (pgrps), a group of pattern recognition receptors (prrs), can recognize peptidoglycan (pgn) of the bacteria cell wall and play an important role in host immune defense against pathogen infection. they are highly structurally conserved through evolution, but with different function in innate immunity between invertebrates and vertebrates. in teleost fish, several pgrps have been characterized recently. they have both amidase activity and bactericidal ac ... | 2016 | 27461422 |
| transcriptomic profiling revealed the signatures of intestinal barrier alteration and pathogen entry in turbot (scophthalmus maximus) following vibrio anguillarum challenge. | the mucosal immune system serves as the frontline barriers of host defense against pathogen infection, especially for the fishes, which are living in the pathogen rich aquatic environment. the intestine constitutes the largest surface body area in constantly contact with the external pathogens, and plays a vital role in the immune defense against inflammation and pathogen infection. previous studies have revealed that fish intestine might serves as the portal of entry for vibrio anguillarum. to ... | 2016 | 27431928 |
| immunohistochemical study of inducible nitric oxide synthase and tumour necrosis factor alpha response in turbot (scophthalmus maximus) experimentally infected with aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida represents one of the major threats in aquaculture, especially in salmonid fish and turbot farming. in order to fight bacterial infections, fish have an immune system composed by innate and specific cellular and humoral elements analogous to those present in mammals. however, innate immunity plays a primordial role against bacterial infections in teleost fish. among these non-specific mechanisms, the production of nitric oxide (no) by inducible nitric oxi ... | 2016 | 27431586 |
| involvement of acylated homoserine lactones (ahls) of aeromonas sobria in spoilage of refrigerated turbot (scophthalmus maximus l.). | one quorum sensing strain was isolated from spoiled turbot. the species was determined by 16s rrna gene analysis and classical tests, named aeromonas sobria as7. quorum-sensing (qs) signals (n-acyl homoserine lactones (ahls)) were detected by report strains and their structures were further determined by gc-ms. the activity changes of ahls on strain growth stage as well as the influence of different culture conditions on secretion activity of ahls were studied by the punch method. the result ind ... | 2016 | 27420072 |
| identification and expression analysis of tlr2 in mucosal tissues of turbot (scophthalmus maximus l.) following bacterial challenge. | the pathogen recognition receptors (prrs), which can recognize the conserved pathogen-associated molecular patterns (pamps) of the bacteria, play key roles in the mucosal surfaces for pathogen recognition and activation of immune signaling pathways. however, our understanding of the prrs and their activities in mucosal surfaces in the critical early time points during pathogen infection is still limited. towards to this end, here, we sought to identify the toll-like receptor 2 (tlr2) in turbot a ... | 2016 | 27368539 |
| turbot (scophthalmus maximus) vs. vhsv (viral hemorrhagic septicemia virus): a review. | turbot (scophthalmus maximus) is a very valuable fish species both in europe and china. the culture of this flatfish is well-established but several bacteria, viruses, and parasites can produce mortality or morbidity episodes in turbot farms. viral hemorrhagic septicemia virus (vhsv) is one of the most threatening pathogens affecting turbot, because neither vaccines nor treatments are commercially available. although the mortality in the turbot farms is relatively low, when this virus is detecte ... | 2016 | 27303308 |
| the mucosal expression signatures of g-type lysozyme in turbot (scophthalmus maximus) following bacterial challenge. | the mucosal surfaces constitute the first line of host defense against infection, and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes, while in constantly contact with a wide range of pathogens. the lysozymes are considered as key components for innate immune response to pathogen infection with their strong antibacterial activities. but their activities in mucosal immune responses were always overlooked, especially for g-type l ... | 2016 | 27189917 |
| reassortant betanodavirus infection in turbot (scophthalmus maximus). | in this study, the susceptibility of turbot juveniles to two betanodavirus strains was assessed, a rgnnv/sjnnv reassortant (ss160.03) and a sjnnv strain. the reassortant isolate exhibits a slightly modified sjnnv cp, with two amino acid substitutions in the c-terminal domain (positions 247 and 270). to analyse the role of these residues as virulence and host determinants in turbot, three recombinant strains (rss160.03247 , rss160.03270 , rss160.03247+270 ) harbouring site-specific mutations in t ... | 2016 | 27135777 |
| characterization of n-acyl homoserine lactones (ahls) producing bacteria isolated from vacuum-packaged refrigerated turbot (scophthalmus maximus) and possible influence of exogenous ahls on bacterial phenotype. | quorum sensing (qs) is a cell-to-cell communication mechanism through which microbial cells communicate and regulate their wide variety of biological activities. n-acyl homoserine lactones (ahls) are considered to be the most important qs signaling molecules produced by several gram-negative bacteria. the present study aimed to screen the ahls-producing bacteria from spoiled vacuum-packaged refrigerated turbot (scophthalmus maximus) by biosensor assays, and the profiles of ahls produced by these ... | 2016 | 27118073 |
| fast real time pcr for control of intra-species recycling in aquaculture feed, focused to the most relevant fish species farmed in europe. | recent regulations in animal feed composition prohibit intra-species recycling, the recycling of one given animal species to the same species, in order to avoid potential safety risks to human and animal health. these regulations have generated the need of their control in aquaculture by effective and specific analytical techniques. to date, most studies of species identification and detection in feedstuffs are focused on land species, but few studies are focused on species composition in fish f ... | 2016 | 26988512 |
| adjuvant effect of quillaja saponaria saponin (qss) on protective efficacy and igm generation in turbot (scophthalmus maximus) upon immersion vaccination. | the adjuvant effect of quillaja saponaria saponin (qss) on protection of turbot fry was investigated with immersion vaccination of formalin-killed vibrio anguillarum o1 and various concentrations of qss (5, 25, 45 and 65 mg/l). fish were challenged at days 7, 14 and 28 post-vaccination. significantly high relative percent of survival (rps) ((59.1 ± 13.6)%, (81.7 ± 8.2)%, (77.8 ± 9.6)%) were recorded in the fish that received bacterins immersion with qss at 45 mg/l, which is comparable to the pos ... | 2016 | 26950114 |
| development and application of monoclonal antibodies for detection and analysis of aquareoviruses. | monoclonal antibodies (mabs) play an important role in detection of aquareoviruses. three mabs against grass carp reovirus (gcrv) were prepared. isotyping revealed that all three mabs were of subclass igg2b. western blot assay showed that all three mabs reacted with gcrv 69 kda protein (the putative vp5). in addition to the 69 kda protein of gcrv, mab 4b6 also recognize a 54 kda protein. all three mabs were used for detecting aquareovirus by western blot assay and indirect immunofluorescence ass ... | 2016 | 26889962 |
| helmintofauna of turbot scophthalmus maximus (linnaeus, 1758) from the southern baltic sea including new data. | turbot scophthalmus maximus (linnaeus, 1758) is a fish belonging to the pleuronectiformes order. it is commonly observed in waters of the northern atlantic, and also in the baltic sea. as an economically significant species, it is fished on an industrial scale, and also farmed in some european countries. seventy-two turbots from the gulf of gdańsk (26th ices zone) were examined for parasite presence in the years 2010-2012. the study revealed the presence of the tapeworm bothriocephalus scorpii ( ... | 2015 | 26618593 |
| dietary β-glucan (macrogard®) enhances survival of first feeding turbot (scophthalmus maximus) larvae by altering immunity, metabolism and microbiota. | reflecting the natural biology of mass spawning fish aquaculture production of fish larvae is often hampered by high and unpredictable mortality rates. the present study aimed to enhance larval performance and immunity via the oral administration of an immunomodulator, β-glucan (macrogard(®)) in turbot (scophthalmus maximus). rotifers (brachionus plicatilis) were incubated with or without yeast β-1,3/1,6-glucan in form of macrogard(®) at a concentration of 0.5 g/l. rotifers were fed to first fee ... | 2016 | 26564474 |
| tissue localization of lymphocystis disease virus (lcdv) receptor-27.8 kda and its expression kinetics induced by the viral infection in turbot (scophthalmus maximus). | the 27.8 kda membrane protein expressed in flounder (paralichthys olivaceus) gill cells was proved to be a receptor mediating lymphocystis disease virus (lcdv) infection. in this study, sds-page and western blotting demonstrated that 27.8 kda receptor (27.8r) was shared by flounder and turbot (scophthalmus maximus). indirect immunofluorescence assay (iifa) and immunohistochemistry showed that 27.8r was widely expressed in tested tissues of healthy turbot. the indirect enzyme-linked immunosorbent ... | 2015 | 26556346 |
| whole-genome analysis of a novel fish reovirus (msrev) discloses aquareovirus genomic structure relationship with host in saline environments. | aquareoviruses are serious pathogens of aquatic animals. here, genome characterization and functional gene analysis of a novel aquareovirus, largemouth bass micropterus salmoides reovirus (msrev), was described. it comprises 11 dsrna segments (s1-s11) covering 24,024 bp, and encodes 12 putative proteins including the inclusion forming-related protein ns87 and the fusion-associated small transmembrane (fast) protein ns22. the function of ns22 was confirmed by expression in fish cells. subsequentl ... | 2015 | 26247954 |
| pseudomonas fluorescens: identification of fur-regulated proteins and evaluation of their contribution to pathogenesis. | pseudomonas fluorescens is a gram-negative bacterium and a common pathogen to a wide range of farmed fish. in a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of p. fluorescens (wild-type strain tss). in the present work, we conducted comparative proteomic analysis to examine the global protein profiles of tss and the p. fluorescens fur knockout mutant tfm. twenty-eight differentially produced proteins were identi ... | 2015 | 26119301 |
| molecular cloning and expression analysis of interferon stimulated gene 15 (isg15) in turbot, scophthalmus maximus. | the interferon stimulated gene 15 (isg15) is strongly induced in many cell types by double-stranded rna (polyinosinic: polycytidylic acid, poly i:c) and viral infection. in this study, we described the nucleotide, mrna tissue distribution and regulation of an isg15 gene from turbot, scophthalmus maximus (smisg15). smisg15 gene is 862 bp in length, composed of two exons and one intron, and encodes 158 amino acids. the deduced protein exhibits the highest homology (44.7-71.2% identity) with isg15s ... | 2015 | 26095010 |
| molecular cloning and expression studies of the adapter molecule myeloid differentiation factor 88 (myd88) in turbot (scophthalmus maximus). | myeloid differentiation factor 88 (myd88) is an adapter protein involved in the interleukin-1 receptor (il-1r) and toll-like receptor (tlr)-mediated activation of nuclear factor-kappab (nf-κb). in this study, a full length cdna of myd88 was cloned from turbot, scophthalmus maximus. it is 1619 bp in length and contains an 858-bp open reading frame that encodes a peptide of 285 amino acid residues. the putative turbot (sm)myd88 protein possesses a n-terminal death domain and a c-terminal toll/il-1 ... | 2015 | 26025195 |
| p247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection. | megalocytivirus is a dna virus with a broad host range among teleost fish. although the complete genome sequences of a number of megalocytivirus isolates have been reported, the functions of most of the genes of this virus are unknown. in this study, we selected two megalocytivirus immunogens, p247 and p523, which were expressed during host infection and, when in the form of dna vaccines (pcn247 and pcn523 respectively), elicited strong protectivity against lethal megalocytivirus challenge in a ... | 2015 | 25815484 |
| high-density genetic linkage mapping in turbot (scophthalmus maximus l.) based on snp markers and major sex- and growth-related regions detection. | this paper describes the development of a high density consensus genetic linkage map of a turbot (scophthalmus maximus l.) family composed of 149 mapping individuals using single nucleotide polymorphisms (snp) developed using the restriction-site associated dna (rad) sequencing technique with the restriction enzyme, psti. a total of 6,647 snps were assigned to 22 linkage groups, which is equal to the number of chromosome pairs in turbot. for the first time, the average marker interval reached 0. ... | 2015 | 25775256 |
| cloning and expression analysis of a toll-like receptor 22 (tlr22) gene from turbot, scophthalmus maximus. | toll-like receptor 22 (tlr22) exists exclusively in aquatic animals and recognizes double stranded rna (dsrna). in the present study, a tlr22 gene and its 5'-flanking sequence were cloned from turbot, scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. the turbot (sm)tlr22 gene spans over 5.6 kb with a structure of 4 exon-3 intron and encodes 962 amino acids. the deduced protein shows the highest sequence identity (76.7%) to japanese flounder tlr22 and posses ... | 2015 | 25770871 |
| pseudomonas fluorescens: iron-responsive proteins and their involvement in host infection. | for pathogenic bacteria, the ability to acquire iron is vital to survival in the host. in consequence, many genes involved in iron acquisition are associated with bacterial virulence. pseudomonas fluorescens is a bacterial pathogen to a variety of farmed fish. however, the global regulatory function of iron in pathogenic p. fluorescens is essentially unknown. in this study, in order to identify proteins affected by iron condition at the expression level, we performed proteomic analysis to compar ... | 2015 | 25680811 |
| a toll-like receptor 3 homologue that is up-regulated by poly i:c and dna virus in turbot scophthalmus maximus. | in this study, the gene and promoter sequences of turbot scophthalmus maximus (sm) toll-like receptor 3 (tlr3) were cloned and its mrna tissue distribution and gene expression in response to polyinosinic:polycytidylic acid (poly i:c) and turbot reddish body iridovirus (trbiv) challenges were studied in vivo. the smtlr3 gene spans over 4·4 kb with a structure of five exons-four introns and encodes a peptide of 916 amino acids. the putative protein shares the highest sequence identity of 52·8-78·5 ... | 2015 | 25631305 |
| a genetic linkage map of sole (solea solea): a tool for evolutionary and comparative analyses of exploited (flat)fishes. | linkage maps based on markers derived from genes are essential evolutionary tools for commercial marine fish to help identify genomic regions associated with complex traits and subject to selective forces at play during exploitation or selective breeding. additionally, they allow the use of genomic information from other related species for which more detailed information is available. sole (solea solea l.) is a commercially important flatfish species in the north sea, subject to overexploitatio ... | 2014 | 25541971 |
| identification, expression and immunological responses to bacterial challenge following vaccination of blt1 gene from turbot, scophthalmus maximus. | leukotriene b4 (ltb4) is well known as a chemoattractant for leucocytes, recent studies also showed its involvement in adaptive immunity. the purpose of this work is to report the cloning, characterization and gene expression of leukotriene b4 receptor (blt1) in turbot (scophthalmus maximus), as well as the immunological response to challenge following vaccination with a live attenuated vaccine vibrio anguillarum mvav6203. the full cdna sequence of turbot blt1 was cloned. the open reading frame ... | 2015 | 25541026 |
| in vitro and in vivo evaluation of lactic acid bacteria of aquatic origin as probiotics for turbot (scophthalmus maximus l.) farming. | turbot (scophthalmus maximus l.) is an important commercial marine flatfish. its production may be affected by bacterial diseases that cause severe economical losses, mainly tenacibaculosis and vibriosis, provoked by tenacibaculum maritimum and vibrio splendidus, respectively. an alternative or complementary strategy to chemotherapy and vaccination for the control of these diseases is the use of probiotics. in this work, we report the in vitro and in vivo potential of eight lactic acid bacteria ... | 2014 | 25451001 |
| an invasive and low virulent edwardsiella tarda esrb mutant promising as live attenuated vaccine in aquaculture. | edwardsiella tarda is a leading fish pathogen haunting worldwide aquaculture industry. in e. tarda, two-component system esra-esrb positively regulates type iii and vi secretion systems (t3ss and t6ss) and negatively regulates hemolysin etha, which has been demonstrated to be essential for the invasion processes in fish. in order to develop a live attenuated vaccine (lav) with high invasiveness to be practically and economically used as immersion-administered vaccine in aquaculture, here, we gen ... | 2015 | 25431010 |
| increased survival of juvenile turbot scophthalmus maximus by using bacteria associated with cultured oysters. | preventing vibriosis in juvenile cultured turbot scophthalmus maximus caused by vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. whether the use of probiotics instead of antibiotics in juvenile turbot is a safer strategy requires more study. the antibacterial potential of 148 vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and turbot) was analyzed in vitro against v. anguillarum and other pathogens by mean ... | 2014 | 25361445 |
| molecular characterization of the cathepsin b of turbot (scophthalmus maximus). | cathepsin b is an enzymatic protein belonging to the peptidase c1 family. it is involved in diverse physiological and pathological functions that include immune response. in this study, we identified and characterized a cathepsin b homolog (smcatb) from turbot (scophthalmus maximus). smcatb is composed of 330 amino acid residues and possesses typical domain architecture of cathepsin b, which contains a propeptide region and a cysteine protease domain, and the latter processes four conserved resi ... | 2015 | 25326658 |
| involvement of fish signal transducer and activator of transcription 3 (stat3) in sgiv replication and virus induced paraptosis. | signal transducer and activator of transcription 3 (stat3) is an important transcription factor which plays crucial roles in immune regulation, inflammation, cell proliferation, transformation, and other physiological processes of the organism. in this study, a novel stat3 gene from orange spotted grouper (ec-stat3) was cloned and characterized. bioinformatic analysis revealed that full-length of ec-stat3 was 3105-bp long and contained a 280-bp 5'utr, a 470-bp 3'utr, and a 2355-bp open reading f ... | 2014 | 25230133 |
| nano-gold capillary immunochromatographic assay for parvalbumin. | a novel non-instrumental bioanalysis based on colloidal-gold immunochromatography in a modified glass capillary was developed and named capillary immunochromatographic assay (cica). in this report, glass capillary was proposed as a support in immunochromatographic assay because of its excellent characteristics. goat anti-rabbit igg and parvalbumin (pv) were immobilized on the inner wall of the glass capillary as control zone and test zone, respectively. the cica was constructed, and main variabl ... | 2014 | 25168115 |
| identification and analysis of three virulence-associated tonb-dependent outer membrane receptors of pseudomonas fluorescens. | pseudomonas fluorescens is a gram-negative bacterium that can infect a wide range of farmed fish. however, very little is known about the virulence mechanism of p. fluorescens as a fish pathogen. in this study, we identified and analyzed 3 tonb-dependent outer membrane receptors (tdrs) from a pathogenic p. fluorescens strain isolated from fish. in silico analysis revealed that all 3 proteins (named tdr1 to 3) possess structural domains typical of tdrs. quantitative real time rt-pcr analysis show ... | 2014 | 25114042 |
| transcriptome profiles associated to vhsv infection or dna vaccination in turbot (scophthalmus maximus). | dna vaccines encoding the viral g glycoprotein show the most successful protection capability against fish rhabdoviruses. nowadays, the molecular mechanisms underlying the protective response remain still poorly understood. with the aim of shedding light on the protection conferred by the dna vaccines based in the g glycoprotein of viral haemorrhagic septicaemia virus (vhsv) in turbot (scophthalmus maximus) we have used a specific microarray highly enriched in antiviral sequences to carry out th ... | 2014 | 25098168 |
| molecular cloning and characterization of secretory and membrane-bound igm of turbot. | in recent years, increasing diseases especially bacterial diseases have brought a host of losses with the expansive cultivation of turbot (scophthalmus maximus). in order to do more research about the immune system of turbot for better understanding the mechanism of resisting diseases, the immunoglobulin genes related to secretory and membrane-bound igm (s-igm and m-igm) of turbot were cloned using homology sequences cloning and smart race pcr method. the heavy chain of s-igm cdna is 1900 bp in ... | 2014 | 25066925 |
| the shelf life of farmed turbot (scophthalmus maximus). | a total of 18 farmed turbot (scophthalmus maximus) were slaughtered over 4 successive weeks in november 2012 and stored in polystyrene boxes with ice until analyzed. the fish were stored between 1 and 22 d and presented to a taste panel and further analyzed for quality index method (qim), microbiological analysis by real-time quantitative pcr (qpcr), taste, ph, color by computer imaging, protein denaturation with differential scanner calorimeter (dsc), texture hardness, and shear force. results ... | 2014 | 25046980 |
| the warm temperature acclimation protein (wap65) has an important role in the inflammatory response of turbot (scophthalmus maximus). | wap65 is a molecule similar to the mammalian hemopexin that is a serum glycoprotein produced mainly by the liver with high affinity to heme. its primary role is participating in iron metabolism scavenging heme that is released into the plasma and transporting it to the liver. it has been reported an important role of hemopexin in the inflammation as an acute-phase protein and its production is up-regulated by pro-inflammatory cytokines. there are also some evidences suggesting this immune-induct ... | 2014 | 24794581 |
| sil: a streptococcus iniae bacteriocin with dual role as an antimicrobial and an immunomodulator that inhibits innate immune response and promotes s. iniae infection. | streptococcus iniae is a gram-positive bacterium and a severe pathogen to a wide range of economically important fish species. in addition, s. iniae is also a zoonotic pathogen and can cause serious infections in humans. in this study, we identified from a pathogenic s. iniae strain a putative bacteriocin, sil, and examined its biological activity. sil is composed of 101 amino acid residues and shares 35.6% overall sequence identity with the lactococcin 972 of lactococcus lactis. immunoblot anal ... | 2014 | 24781647 |
| the first characterization of two type i interferons in turbot (scophthalmus maximus) reveals their differential role, expression pattern and gene induction. | type i interferons (ifns) are considered the main cytokines directing the antiviral immune response in vertebrates. these molecules are able to induce the transcription of interferon-stimulated genes (isgs) which, using different blocking mechanisms, reduce the viral proliferation in the host. in addition, a contradictory role of these ifns in the protection against bacterial challenges using murine models has been observed, increasing the survival or having a detrimental effect depending on the ... | 2014 | 24680948 |
| assessment of trace metal concentrations in muscle tissue of certain commercially available fish species from kayseri, turkey. | regular consumption of fish has been widely recommended by health authorities. however, it is known that some species accumulate high levels of contaminants including heavy metals (e.g., hg, cd, pb, and as). in this study, cu, pb, co, ni, cr, mn, cd, and fe were determined in the muscle tissue of 11 commercially available fish species (sparus auratus, dicentrarchus labrax, mullus barbatus, belone belone, psetta maxima, epinephelus aeneus, salmothymus, soleidae, pomatomus saltatrix, engraulis enc ... | 2014 | 24633787 |
| molecular analysis of bacterial communities and detection of potential pathogens in a recirculating aquaculture system for scophthalmus maximus and solea senegalensis. | the present study combined a dgge and barcoded 16s rrna pyrosequencing approach to assess bacterial composition in the water of a recirculating aquaculture system (ras) with a shallow raceway system (srs) for turbot (scophthalmus maximus) and sole (solea senegalensis). barcoded pyrosequencing results were also used to determine the potential pathogen load in the ras studied. samples were collected from the water supply pipeline (sup), fish production tanks (pro), sedimentation filter (sed), biof ... | 2013 | 24278329 |
| molecular cloning and multifunctional characterization of grim-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (scophthalmus maximus). | grim-19 (gene associated with retinoid-interferon-induced mortality 19), a novel cell death regulatory gene, plays important roles in cell apoptosis, embryogenesis, mitochondrial respiratory chain and immune response. to date, little information is known about fish grim-19 characteristics except orange-spotted grouper (epinephelus coioides). here a new grim-19 gene is identified and characterized from turbot (scophthalmus maximus), an economic marine fish in china and europe. briefly, turbot gri ... | 2014 | 24239557 |
| incorporation and metabolism of (14)c-labelled polyunsaturated fatty acids in juvenile gilthead sea bream sparus aurata l. in vivo. | the incorporation, and the capacity for desaturation and elongation in vivo, of intraperitoneally-injected, (14)c-labelled n-3 and n-6 c18 and c20 pufas were investigated in juvenile gilthead sea bream, sparus aurata. the results indicate that juvenile gilthead sea bream have only limited ability to convert ch pufas to c20 and c22 hufas in vivo. the data are consistent with the results from nutritional studies on larvae, postlarvae and fingerlings that have shown that gilthead sea bream require ... | 1993 | 24214444 |
| incorporation and metabolism of(14)c-labelled polyunsaturated fatty acids in wild-caught juveniles of golden grey mullet,liza aurata, in vivo. | the incorporation, and the capacity for desaturation and elongation in vivo, of intraperitoneally-injected,(14)c-labelled n-3 and n-6 c18 and c20 polyunsaturated fatty acids (pufa) were investigated in juvenile golden grey mullet,liza aurata. the results indicate that juvenile mullet have only limited ability to convert c18 polyunsaturated fatty acids to c20 and c22 highly unsaturated fatty acids (hufa)in vivo. this suggests that juvenile golden grey mullet require the provision of preformed c20 ... | 1993 | 24202691 |
| cloning, molecular characterization, and expression analysis of the signal transducer and activator of transcription 3 (stat₃) gene from grass carp (ctenopharyngodon idellus). | signal transducer and activator of transcription 3 (stat₃) binds to janus kinase 2 (jak₂) to initiate the jak₂/stat₃ signal transduction pathway, which plays an important role in cancer cell proliferation, immune regulation, reproduction, lipid metabolism, and other physiological processes of the organism. in this study, the cdna sequence of the stat₃ gene from grass carp was cloned using race (rapid-amplification of cdna ends). twelve characteristics of the stat₃ gene and its encoded protein se ... | 2013 | 24055509 |
| smccl19, a cc chemokine of turbot scophthalmus maximus, induces leukocyte trafficking and promotes anti-viral and anti-bacterial defense. | chemokines are classified into several different subfamilies, of which cc chemokines constitute the largest subfamily in teleost. the prominent structural characteristic of cc chemokines is the presence of an asp-cys-cys-leu (dccl) motif. to date, cdna sequences of several cc chemokines have been identified in turbot (scophthalmus maximus), however, the activity and function of these putative chemokines remain unknown. in this study, we examined the biological effect of the turbot cc chemokine s ... | 2013 | 24012750 |
| immunohistochemical characterization of polyclonal antibodies against enteromyxum leei and enteromyxum scophthalmi (myxozoa: myxosporea), intestinal parasites of fish. | the enteric myxozoan parasites enteromyxum leei (diamant, lom et dyková) and enteromyxum scophthalmi palenzuela, redondo et álvarez-pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream (gsb), sparus aurata l., and to high mortality rates in turbot, psetta maxima (l.). the detection of initial parasite stages in histological sections is particularly difficult, but can be simplified by means of specific ant ... | 2014 | 23957839 |
| molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (stat2) from turbot, scophthalmus maximus. | signal transducer and activator of transcription 2 (stat2) is an important molecule involved in the type i interferon signalling pathway. to date, little stat2 homologue is available in fish except atlantic salmon and goldfish. in this paper, stat2 was firstly cloned and characterized from turbot, a marine flatfish with high economic value. briefly, turbot stat2 cdna is 3206 bp in length encoding a predicted protein of 793 amino acids. the phylogenetic tree shows that turbot stat2 protein shared ... | 2013 | 23933433 |
| effectiveness of probiotic phaeobacter bacteria grown in biofilters against vibrio anguillarum infections in the rearing of turbot (psetta maxima) larvae. | the rearing environment of first-feeding turbot larvae, usually with high larvae densities and organic matter concentrations, may promote the growth of opportunistic pathogenic vibrionaceae bacteria, compromising the survival of the larvae. the aim of this study was to assess the effectiveness of the biofilm-forming probiotic phaeobacter 27-4 strain grown on a ceramic biofilter (probiofilter) in preventing vibrio anguillarum infections in turbot larvae. in seawater with added microalgae and main ... | 2013 | 23917459 |
| evidence for paralichthys olivaceus ifitm1 antiviral effect by impeding viral entry into target cells. | interferon-inducible transmembrane (ifitm) protein family is novel viral restriction factors with representative transmembrane structure. these proteins also exist in fish, however, their roles in the innate immune response remain unknown. here, we report a characterization of teleost ifitm1 from flounder paralichthys olivaceus (poifitm1), which exhibits conserved structure characteristic of the ifitm family but comprises a relatively longer n-terminal region. the expression and promoter activit ... | 2013 | 23850425 |
| edwardsiella tarda ivy, a lysozyme inhibitor that blocks the lytic effect of lysozyme and facilitates host infection in a manner that is dependent on the conserved cysteine residue. | edwardsiella tarda is a gram-negative bacterial pathogen with a broad host range that includes fish and humans. in this study, we examined the activity and function of the lysozyme inhibitor ivy (named ivyet) identified in the pathogenic e. tarda strain tx01. ivyet possesses the ivy signature motif ckphdc in the form of (82)cqphnc(87) and contains several highly conserved residues, including a tryptophan (w55). for the purpose of virulence analysis, an isogenic tx01 mutant, txivy, was created. t ... | 2013 | 23817616 |
| the major fimbrial subunit protein of edwardsiella tarda: vaccine potential, adjuvant effect, and involvement in host infection. | edwardsiella tarda is a gram-negative bacterium that is reckoned one of the most severe fish pathogens. in this study, we analyzed the biological properties of the e. tarda major fimbrial subunit protein, fima. we found that mutation of fima resulted in defective biofilm growth, attenuated infectivity against host cells, and impaired ability to disseminate into and colonize host tissues following experimental infection. when used as a subunit vaccine, recombinant fima (rfima) elicited a high lev ... | 2013 | 23811351 |
| taxonomic and functional metagenomic profiling of gastrointestinal tract microbiome of the farmed adult turbot (scophthalmus maximus). | metagenomics combined with 16s rrna gene sequence analyses was applied to unveil the taxonomic composition and functional diversity of the farmed adult turbot gastrointestinal (gi) microbiome. proteobacteria and firmicutes which existed in both gi content and mucus were dominated in the turbot gi microbiome. 16s rrna gene sequence analyses also indicated that the turbot gi tract may harbor some bacteria which originated from associated seawater. functional analyses indicated that the clustering- ... | 2013 | 23802730 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| effect of feeding selectivity on the transfer of methylmercury through experimental marine food chains. | the present study established an experimental marine food chain of three levels (microalgae → zooplankton → fish) to investigate the effect of feeding selectivity on the transfer of methylmercury (mehg) through the food chain. total hg concentrations in chlorella sp., nitzschia closterium and isochrysis galbana exposed to 1 μg l(-1) mehg for three days were 27.91, 32.74 and 40.03 μg g(-1) respectively and were significantly different (p < 0.05) between species. moina mongolica and artemia sp. pr ... | 2013 | 23702096 |
| screening and analysis of poakirin1 and two related genes in response to immunological stimulants in the japanese flounder (paralichthys olivaceus). | a member of the nf-κb signaling pathway, poakirin1, was cloned from a full-length cdna library of japanese flounder (paralichthys olivaceus). the full-length cdna comprises a 5'utr of 202 bp, an open reading frame of 564 bp encoding a 187-amino-acid polypeptide and a 521-bp 3'utr with a poly (a) tail. the putative protein has a predicted molecular mass of 21 kda and an isoelectric point (pi) of 9.22. amino acid sequence alignments showed that poakirin1 was 99% identical to the scophthalmus maxim ... | 2013 | 23651673 |
| phenotypic characterization, virulence, and immunogenicity of edwardsiella tarda lse40 aroa mutant. | bacterial aro mutants are frequently used as live attenuated vaccines for domestic animals. in this study, we characterized edwardsiella tarda strain lse40 with a deletion in the aroa gene. in addition to autotrophy, the aroa mutant appeared to have delayed cell division and reductions in its swarming motility, biofilm formation, and production of translocator proteins in the type iii secretory system. the mutant exhibited high virulence attenuation in turbot fish, scophthalmus maximus (l.), whe ... | 2013 | 23504078 |
| a combined strategy involving sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot (scophthalmus maximus). | genomic resources for plant and animal species that are under exploitation primarily for human consumption are increasingly important, among other things, for understanding physiological processes and for establishing adequate genetic selection programs. current available techniques for high-throughput sequencing have been implemented in a number of species, including fish, to obtain a proper description of the transcriptome. the objective of this study was to generate a comprehensive transcript ... | 2013 | 23497389 |
| environmental isolates p1sw and v3sw as a bivalent vaccine induce effective cross-protection against edwardsiella tarda and vibrio anguillarum . | edwardsiella tarda and vibrio anguillarum are severe fish pathogens. in this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of e. tarda- and v. anguillarum-associated diseases. for this purpose, we selected and analyzed 2 seawater isolates, p1sw and v3sw, belonging to the genera pseudomonas and vibrio, respectively. when administered to turbot scophthalmus maximus via immersion and oral feeding, p1sw and v3sw at a dose of 2 × 108 colony ... | 2013 | 23482384 |
| inter-species transmission of viral hemorrhagic septicemia virus (vhsv) from turbot (scophthalmus maximus) to rainbow trout (onchorhynchus mykiss). | successful viral infection is a complex mechanism, involving many host-pathogen interactions that developed during coevolution of host and pathogen, and often result in host-species specificity. nevertheless, many viruses are able to infect several host species and sporadically cross species barriers. the viral hemorrhagic septicemia virus (vhsv), a rhabdovirus with high economic impact on the aquaculture industry, has developed an exceptionally wide host range across marine and freshwater envir ... | 2013 | 23239574 |
| quorum-sensing regulates biofilm formation in vibrio scophthalmi. | in a previous study, we demonstrated that vibrio scophthalmi, the most abundant vibrio species among the marine aerobic or facultatively anaerobic bacteria inhabiting the intestinal tract of healthy cultured turbot (scophthalmus maximus), contains at least two quorum-sensing circuits involving two types of signal molecules (a 3-hydroxy-dodecanoyl-homoserine lactone and the universal autoinducer 2 encoded by luxs). the purpose of this study was to investigate the functions regulated by these quor ... | 2012 | 23198796 |
| the effect of fish matrix on the enzyme-linked immunosorbent assay of antibiotics. | the matrix effect is considered to be a problem in the immunoassay of foodstuffs. however, information on the interference from aquatic products, as well as the mechanism involved, is very limited. in this study, using three flatfishes (scophthalmus maximus, paralichthys olivaceus and cymoglossus robustus) as samples, the effect of the fish matrix on the competitive indirect enzyme-linked immunosorbent assay (ci-elisa) of antibiotic (norfloxacin) residues was investigated. the mechanism of the o ... | 2013 | 23150496 |
| immune response of turbot (scophthalmus maximus l.) to a broad spectrum vaccine candidate, recombinant glyceraldehyde-3-phosphate dehydrogenase of edwardsiella tarda. | edwardsiella tarda is a gram-negative pathogen which causes systemic infection in turbot. the increasing frequency of edwardsiellosis in turbot farming has stressed the need to understand the immune responses of fish, for the further development of prevention and control strategies. as a broad spectrum protective antigen, a recombinant glyceraldehyde-3-phosphate dehydrogenase (gapdh) of e. tarda eib202 has been proven to present remarkable protection against e. tarda, aeromonas hydrophila, vibri ... | 2012 | 23084627 |
| characterization of a c-type lysozyme of scophthalmus maximus: expression, activity, and antibacterial effect. | lysozyme is a key component of the innate immune system and plays an important role in antibacterial infection. in this study, we analyzed the expression and activity of a chicken-type (c-type) lysozyme (named smlysc) from turbot (scophthalmus maximus). smlysc is composed of 143 residues and shares 67-90% overall sequence identities with the c-type lysozymes of a number of teleost fish. smlysc possesses a typical c-type lysozyme domain, which contains the conserved residues e50 and d67 that form ... | 2013 | 23063540 |
| characterization and gene expression analysis of the two main th17 cytokines (il-17a/f and il-22) in turbot, scophthalmus maximus. | this report describes the cloning, characterization and gene expression pattern of two th17 cytokines, interleukin (il)-17a/f and -22, in turbot scophthalmus maximus. the turbot il-17a/f cdna contains a 516 bp open reading frame encoding a deduced il-17a/f protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. turbot il-22 had a 564 bp orf coding for a 187 aa protein with a 33 aa signal peptide. the turbot il-22 protein contained a typical il-10 family signature ... | 2012 | 23000268 |
| construction and analysis of experimental dna vaccines against megalocytivirus. | iridoviruses are large double-stranded dna viruses with icosahedral capsid. the iridoviridae family contains five genera, one of which is megalocytivirus. megalocytivirus has emerged in recent years as an important pathogen to a wide range of marine and freshwater fish. in this study, we aimed at developing effective genetic vaccines against megalocytivirus affecting farmed fish in china. for this purpose, we constructed seven dna vaccines based on seven genes of rock bream iridovirus isolate 1 ... | 2012 | 22986024 |
| cloning, characterization and expression analysis of a cold shock domain family member yb-1 in turbot scophthalmus maximus. | the y-box proteins are a family of highly conserved nucleic acid binding proteins. in this report we have identified a new member, yb-1 from turbot (scophthalmus maximus) spleen cdna library. the full-length cdna sequence of turbot yb-1 was obtained and then the expression at transcriptional level was researched by qrt-pcr. in normal organs, the expression of yb-1 was higher in liver, brain, gill and heart, respectively. yb-1 had the highest expression level at gastrula stage during the early st ... | 2012 | 22982328 |
| microarray-based identification of differentially expressed genes in families of turbot (scophthalmus maximus) after infection with viral haemorrhagic septicaemia virus (vhsv). | viral haemorrhagic septicaemia virus (vhsv) is one of the major threats to the development of the aquaculture industry worldwide. the present study was aimed to identify genes differentially expressed in several turbot (scophthalmus maximus) families showing different mortality rates after vhsv. the expression analysis was conducted through genome-wide expression profiling with an oligo-microarray in the head kidney. a significant proportion of the variation in the gene expression profiles seeme ... | 2012 | 22790792 |
| an edwardsiella tarda mutant lacking udp-glucose dehydrogenase shows pleiotropic phenotypes, attenuated virulence, and potential as a vaccine candidate. | edwarsiella tarda is highly resistant to the action of cationic antimicrobial peptides (camps). however, the mechanism underlying camp resistance is not clear. the enzyme udp-glucose dehydrogenase (ugd) that converts udp-glucose into udp-glucuronic acid may be important for this resistance. in this study, a ugd gene was identified in e. tarda and its functional role was analyzed using an in-frame deletion mutant δugd and the complemented strain ugd+. the lipopolysaccharide (lps) produced by δugd ... | 2012 | 22748630 |
| virulence factors of aeromonas salmonicida subsp. salmonicida strains associated with infections in turbot psetta maxima. | virulence factors for aeromonas salmonicida subsp. salmonicida (ass) strains isolated from cultured turbot psetta maxima l. are unknown with regard to this host. the presence of virulence genes associated with different stages of ass infection in salmonids (vapa, tapa, fla, ascv, ascc, aext, sata and aspa) was analysed using a polymerase chain reaction (pcr) technique in ass strains isolated from turbot. other ass strains isolated from salmonids and environmental a. salmonicida (as) strains were ... | 2012 | 22691983 |
| high-throughput sequence analysis of turbot (scophthalmus maximus) transcriptome using 454-pyrosequencing for the discovery of antiviral immune genes. | turbot (scophthalmus maximus l.) is an important aquacultural resource both in europe and asia. however, there is little information on gene sequences available in public databases. currently, one of the main problems affecting the culture of this flatfish is mortality due to several pathogens, especially viral diseases which are not treatable. in order to identify new genes involved in immune defense, we conducted 454-pyrosequencing of the turbot transcriptome after different immune stimulation ... | 2012 | 22629298 |
| horizontal transmission of nervous necrosis virus between turbot scophthalmus maximus and atlantic cod gadus morhua using cohabitation challenge. | experimental horizontal transmission of nervous necrosis virus (nnv) originating from halibut hippoglossus hippoglossus was studied through cohabitation of intraperitoneally (i.p.) injected fish with uninfected fish for 125 d. the experimental groups consisted of i.p. injected turbot scophthalmus maximus or i.p. injected atlantic salmon salmo salar with turbot, salmon or atlantic cod gadus morhua cohabitants. the initial weights were cod 10 g, salmon 40 g and turbot 3 g. nnv was detected in brai ... | 2012 | 22585299 |
| elemental composition of four farmed fish produced in portugal. | farmed gilthead sea bream (sparus aurata), european sea bass (dicentrarchus labrax), rainbow trout (oncorhynchus mykiss) and turbot (psetta maxima) produced in portugal were analysed in order to characterize their elemental composition. atomic absorption (flame and cold vapour) and molecular absorption spectrometry techniques were used to determine all the studied elements. similar patterns of macro, trace and ultra trace elements were observed for all fish species. the main elements were potass ... | 2012 | 22574998 |
| protection and antibody response induced by intramuscular dna vaccine encoding for viral haemorrhagic septicaemia virus (vhsv) g glycoprotein in turbot (scophthalmus maximus). | turbot (scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (vhsv) strains. to evaluate the possibility of controlling the outbreaks of this infectious disease by means of dna vaccination, the gpg of a vhsv isolated from farmed turbot (vhsv(860)) was cloned into an expression plasmid containing the human cytomegalovirus (cmv) promoter (pmcv1.4-g(860)). in our experimen ... | 2012 | 22554577 |
| molecular characterization, immune responses and dna protection activity of rock bream (oplegnathus fasciatus), peroxiredoxin 6 (prx6). | in this study, we describe the molecular characterization, immune responses of rock bream, oplegnathus fasciatus peroxiredoxin 6 cdna (rbprx6) and dna protection activity of its recombinant protein. the full-length cdna sequence of rbprx6 was identified after pyrosequencing of rock bream cdna library. rbprx6 consists of 663 bp open reading frame (orf) that codes for a putative protein of 221 amino acids with predicted molecular mass of 27 kda. it showed characteristic peroxiredoxin super-family ... | 2012 | 22484606 |
| a divalent dna vaccine based on sia10 and ompu induces cross protection against streptococcus iniae and vibrio anguillarum in japanese flounder. | streptococcosis and vibriosis caused by streptococcus iniae and vibrio anguillarum respectively have affected fish culture industries around the world. previous studies have indicated that the s. iniae antigen sia10 and the v. anguillarum outer membrane protein ompu, when used as dna vaccines, induce protection in turbot (scophthalmus maximus) and asian seabass (lates calcarifer) respectively. in this study, with an effort to develop effective vaccines against s. iniae and v. anguillarum, we con ... | 2012 | 22480661 |
| an aerolysin-like enterotoxin from vibrio splendidus may be involved in intestinal tract damage and mortalities in turbot, scophthalmus maximus (l.), and cod, gadus morhua l., larvae. | vibrio splendidus is a pathogen that can cause major losses during the early stages of larval turbot rearing when live feed (rotifers or artemia) is used. as haemolytic bacteria have often been associated with larval rearing losses, we studied the role of the v. splendidus haemolysin in infection of larvae. from a bank of over 10 000 transposon mutants of v. splendidus, two different types of haemolysin-negative mutants were obtained. both had lost virulence for larval fish, and immunohistochemi ... | 2012 | 22233514 |
| cscxce1: a novel cynoglossus semilaevis cxc chemokine that functions as a chemoattractant and an immunomodulator for peripheral blood leukocytes. | chemokines are small cytokines that, based on their structural differences, are classified into four groups, one of which is called cxc chemokines. in this study, we identified a cxc chemokine, cscxce1, from half-smooth tongue sole (cynoglossus semilaevis) and analyzed its function. the deduced amino acid sequence of cscxce1 contains 115 residues and is phylogenetically distinct from known cxc chemokines. cscxce1 possesses the conserved rcxc motif in the form of rcwc but lacks the elr sequence t ... | 2012 | 22210524 |
| Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2011-30 September 2011. | This article documents the addition of 299 microsatellite marker loci and nine pairs of single-nucleotide polymorphism (SNP) EPIC primers to the Molecular Ecology Resources (MER) Database. Loci were developed for the following species: Alosa pseudoharengus, Alosa aestivalis, Aphis spiraecola, Argopecten purpuratus, Coreoleuciscus splendidus, Garra gotyla, Hippodamia convergens, Linnaea borealis, Menippe mercenaria, Menippe adina, Parus major, Pinus densiflora, Portunus trituberculatus, Procontar ... | 2012 | 22136175 |
| quantitative and qualitative evaluation of inos expression in turbot (psetta maxima) infected with enteromyxum scophthalmi. | enteromyxum scophthalmi is the causative agent of turbot enteromyxosis, an intestinal parasitisation that produces severe desquamative enteritis leading to a cachectic syndrome and eventually the death. it is well known the importance of the innate immune response against parasites in fish, with the release of antimicrobial substances such as reactive oxygen and nitrogen species, produced by the inducible nitric oxide synthase (inos). this enzyme is mainly found in phagocytes, but also in struct ... | 2012 | 22119937 |
| Molecular characterization and expression analysis of interferon regulatory factor 5 (IRF-5) in turbot, Scophthalmus maximus. | Interferon regulatory factor 5 (IRF-5) plays a role both in the antiviral and inflammatory responses. In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-5 gene from turbot, Scophthalmus maximus (SmIRF-5). The gene sequence of SmIRF-5 is 4275 bp long, composed of 9 exons and 8 introns similar to known IRF-5 genes of vertebrates, and encodes a peptide of 487 amino acids. The deduced protein sequence shares the highest identity of ~60-70% with fish IRF-5 an ... | 2012 | 22062122 |
| QTL detection for Aeromonas salmonicida resistance related traits in turbot (Scophthalmus maximus). | Interactions between fish and pathogens, that may be harmless under natural conditions, often result in serious diseases in aquaculture systems. This is especially important due to the fact that the strains used in aquaculture are derived from wild strains that may not have had enough time to adapt to new disease pressures. The turbot is one of the most promising European aquaculture species. Furunculosis, caused by the bacterium Aeromonas salmonicida, produces important losses to turbot industr ... | 2011 | 22047500 |
| Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima). | Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were ... | 2011 | 21925757 |
| Colonization of turbot tissues by virulent and avirulent Aeromonas salmonicida subsp. salmonicida strains during infection. | Preventing disease outbreaks in cultured turbot Psetta maxima L. caused by Aeromonas salmonicida subsp. salmonicida (ASS) requires a better understanding of how this pathogen colonizes its host. Distribution of 1 virulent and 2 avirulent ASS strains in turbot tissues was investigated during early and late stages of infection following an immersion challenge. To track bacteria within the turbot, the ASS strains were tagged with green fluorescent protein (GFP). Both virulent and avirulent strains ... | 2011 | 21848125 |
| an irf-3 homolog that is up-regulated by dna virus and poly i:c in turbot, scophthalmus maximus. | in this study, we described the structure, mrna tissue distribution and regulation of an irf-3 gene from turbot, scophthalmus maximus (smirf-3). the gene sequence of smirf-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known irf-3 genes of fish, and encodes a peptide of 466 amino acids. the deduced protein sequence shares the highest identity of 56.0-81.2% with fish irf-3 and possesses a dna-binding domain (dbd), an irf association domain (iad) and a serine-rich domain (srd) k ... | 2011 | 21782955 |
| molecular characterization, phylogeny, and expression pattern of c-type lysozyme in kelp grouper, epinephelus bruneus. | lysozymes are the key molecules in innate immune system and possess high bactericidal properties. in the present study, a full-length c-type lysozyme cdna has been cloned and characterized by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques from kelp grouper, epinephelus bruneus (eb-ctl). the cdna consists of 753 base pairs (bp) with a 432 bp open reading frame (orf) that encodes 144 amino acids (aa) residues and polyadenylation signal sequence aataaa. the dedu ... | 2011 | 21752343 |
| an inflammatory cc chemokine of cynoglossus semilaevis is involved in immune defense against bacterial infection. | chemokines are a family of small cytokines that regulate leukocyte migration. based on the arrangement of the first two cysteine residues, chemokines are classified into four groups called cxc(+¦), cc(+¦), c, and cx(3)c. in this study, we identified a cc chemokine, cscck1, from half-smooth tongue sole (cynoglossus semilaevis) and analyzed its biological activity. the deduced amino acid sequence of cscck1 contains 111 amino acid residues and is phylogenetically belonging to the ccl19/21/25 group ... | 2011 | 21723394 |
| role of scuticociliate proteinases in infection success in turbot, psetta maxima (l.). | scuticociliatosis caused by philasterides dicentrarchi is one of the most severe diseases of farmed turbot, psetta maxima (l.). immunized fish showed elevated levels of specific antibodies (ab), which caused the destruction of parasites through activation of complement by the alternative and classical pathways (acp and ccp). by using affinity chromatography on bacitracin-sepharose columns, we demonstrated the existence of high levels of parasite proteinases in the serum and, to a lesser extent, ... | 2011 | 21711365 |
| turbot reovirus (smrev) genome encoding a fast protein with a non-aug start site. | a virus was isolated from diseased turbot scophthalmus maximus in china. biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus aquareovirus, and was named scophthalmus maximus reovirus (smrev). to the best of our knowledge, no complete sequence of an aquareovirus from marine fish has been determined. therefore, the complete characterization and analysis of the genome of this novel aquareovirus will facilitate further und ... | 2011 | 21689389 |