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one particular anaplasma phagocytophilum ecotype infects cattle in the camargue, france.anaplasma phagocytophilum is a zoonotic tick-borne pathogen responsible for granulocytic anaplasmosis, a mild to a severe febrile disease that affects man and several animal species, including cows and horses. in europe, i. ricinus is the only proven vector for this pathogen, but studies suggest that other tick genera and species could be involved in its transmission. our objective was to assess the presence and genetic diversity of a. phagocytophilum in domestic animals and different tick speci ...201728764743
babesia, theileria, and hepatozoon species in ticks infesting animal hosts in romania.babesia spp., theileria spp., and hepatozoon spp. are tick-transmitted apicomplexan parasites that cause several important diseases in animals. to increase current knowledge about the diversity of tick-transmitted pathogens in romania, we investigated the occurrence of babesia spp., theileria spp., and hepatozoon spp. in a wide range of tick species infesting animal hosts. we collected 852 ticks from 10 different animal species from 20 counties in romania. the assessment was based on detection o ...201728676905
a comparative test of ixodid tick identification by a network of european researchers.this study reports the results of a comparative test of identification of ticks occurring in western europe and northern africa. a total of 14 laboratories were voluntarily enrolled in the test. each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: dermacentor marginatus, d. reticulatus, haemaphysalis punctata, hyalomma lusitanicum, hy. marginatum, ixodes ricinus, i. hexagonus, rhipicephalus annulatus, r. bursa, r. rossicus, and/or r. sanguineus s.l. tic ...201728320640
borrelia diversity and co-infection with other tick borne pathogens in ticks.identifying borrelia burgdorferi as the causative agent of lyme disease in 1981 was a watershed moment in understanding the major impact that tick-borne zoonoses can have on public health worldwide, particularly in europe and the usa. the medical importance of tick-borne diseases has long since been acknowledged, yet little is known regarding the occurrence of emerging tick-borne pathogens such as borrelia spp., anaplasma phagocytophilum, rickettsia spp., bartonella spp., "candidatus neoehrlichi ...201728261565
detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy.tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ...201728110915
presence of borrelia turdi and borrelia valaisiana (spirochaetales: spirochaetaceae) in ticks removed from birds in the north of spain, 2009-2011.the genus borrelia includes species responsible for severe human diseases such as lyme disease. birds are involved in their epidemiology as dispersers of infected ticks (ixodida: ixodidae) and as reservoirs or amplifiers of the bacterium. herein, the presence of borrelia burgdorferi sensu lato (s.l.) johnson, schmid, hyde, steigerwalt & brenner in 336 ticks collected from birds in the north of spain from 2009 to 2011 was investigated. nucleic acid extracts from 174 ixodes frontalis (panzer), 108 ...201728082654
tick-borne diseases in turkey: a review based on one health perspective.the importance of tick-borne diseases is increasing all over the world, including turkey. global warming, environmental and ecological changes and the existence of suitable habitats increase the impact of ticks and result in frequent emergence or re-emergence of tick-borne diseases (tbds) with zoonotic characteristics. in turkey, almost 19 tbds have been reported in animals and men, involving four protozoa (babesiosis, theileriosis, cytauxzoonosis, hepatozoonosis), one filarial nematode (acantho ...201627977689
dna of piroplasms of ruminants and dogs in ixodid bat ticks.in this study 308 ticks (ixodes ariadnae: 26 larvae, 14 nymphs, five females; i. vespertilionis: 89 larvae, 27 nymphs, eight females; i. simplex: 80 larvae, 50 nymphs, nine females) have been collected from 200 individuals of 17 bat species in two countries, hungary and romania. after dna extraction these ticks were molecularly analysed for the presence of piroplasm dna. in hungary i. ariadnae was most frequently identified from bat species in the family vespertilionidae, whereas i. vespertilion ...201627930692
urban breeding corvids as disseminators of ticks and emerging tick-borne pathogens.crows (corvidae) are common city dwellers worldwide and are increasingly important subjects of epidemiology studies. although their importance as hosts and transmitters of a number of zoonotic parasites and pathogens is well known, there are no studies on their importance as tick hosts. after mosquitoes, ticks are the most important vectors of zoonotic pathogens, especially for those causing emerging zoonotic diseases. pathogenic bacteria, especially borrelia spp., rickettsia spp., and anaplasma ...201727922802
ticks (acari: ixodidae) infesting cattle and some other domestic and wild hosts on the french mediterranean island of corsica.corsica is a mountainous french island in the north-western mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. despite a favourable context for ticks and tick-borne diseases (tbds), the tick fauna of corsica has not systematically been investigated.201627842608
presence of borrelia turdi and borrelia valaisiana (spirochaetales: spirochaetaceae) in ticks removed from birds in the north of spain, 2009-2011.the genus borrelia includes species responsible for severe human diseases such as lyme disease. birds are involved in their epidemiology as dispersers of infected ticks (ixodida: ixodidae) and as reservoirs or amplifiers of the bacterium. herein, the presence of borrelia burgdorferi sensu lato (s.l.) johnson, schmid, hyde, steigerwalt & brenner in 336 ticks collected from birds in the north of spain from 2009 to 2011 was investigated. nucleic acid extracts from 174 ixodes frontalis (panzer), 108 ...201627694146
kampinos national park: a risk area for spotted fever group rickettsioses, central poland?ixodid ticks are important vectors of a variety of bacterial and protozoan pathogens which cause infections in humans. in this study, altogether 1041 questing ixodes ricinus (n = 305) and dermacentor reticulatus ticks (n = 736), sympatrically occurring in kampinos national park (kpn), central-east poland, were analyzed by pcr for rickettsia species. overall, the pathogen prevalence in ticks was 27.5 % for i. ricinus and 42.8 % for d. reticulatus. sequencing analysis showed that the first tick sp ...201627631765
tick species, tick-borne pathogens and symbionts in an insular environment off the coast of western france.insular environments provide ideal natural conditions to study disease ecology, especially emerging diseases, due to clear differentiation between local and long-distance transmission. such environments are of particular interest regarding tick-borne pathogens (tbp), since animal exchange with the mainland (along with any ticks they carry) is limited, and because such locations could lie on migratory routes for birds carrying ticks. therefore both tick species and tbp may display different preva ...201627622976
temporal and spatial distribution and species diversity of hard ticks (acari: ixodidae) in the eastern region of caspian sea.ticks are important parasites because of their voracious blood-feeding activity, and being as vectors for various agents of diseases in both human and livestock. this study was conducted in a monthly schedule from october 2014 to december 2015, at 45 study sites in three regions bordering the caspian sea, according to the topography including hillside, plain and coastal areas in collecting ticks from the body of sheep. alfa and beta biodiversity indices were calculated and compared between the t ...201627519473
anaplasma marginale and theileria annulata in questing ticks from portugal.ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...201627394441
ticks infesting domestic dogs in the uk: a large-scale surveillance programme.recent changes in the distribution of tick vectors and the incidence of tick-borne disease, driven variously by factors such as climate change, habitat modification, increasing host abundance and the increased movement of people and animals, highlight the importance of ongoing, active surveillance. this paper documents the results of a large-scale survey of tick abundance on dogs presented to veterinary practices in the uk, using a participatory approach that allows relatively cost- and time-eff ...201627388169
sarcoptes scabiei infestation does not alter the stability of ectoparasite communities.the host represents a heterogeneous ecosystem where multiple parasite species co-occur and interact with each other for space and resources. although these interactions may rule the features of an infracommunity and may shape the infracommunity response to external perturbations, the resilience of ectoparasite communities to new infestations remains poorly explored.201627370780
detection of theileria luwenshuni in sheep from great britain.theileria spp. are tick-borne protozoan parasites of the phylum apicomplexa, order piroplasmida that infect a wide range of wild and domestic animals. in great britain, theileria spp. have been reported from livestock associated with transmission by the tick haemaphysalis punctata. however, these reports have not been associated with disease. this study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north kent.201627075661
tick-borne infections in human and animal population worldwide.the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...201527047089
perspectives on modelling the distribution of ticks for large areas: so far so good?this paper aims to illustrate the steps needed to produce reliable correlative modelling for arthropod vectors, when process-driven models are unavailable. we use ticks as examples because of the (re)emerging interest in the pathogens they transmit. we argue that many scientific publications on the topic focus on: (i) the use of explanatory variables that do not adequately describe tick habitats; (ii) the automatic removal of variables causing internal (statistical) problems in the models withou ...201627030357
molecular identification of borrelia genus in questing hard ticks from portugal: phylogenetic characterization of two novel relapsing fever-like borrelia sp.in the last decades, several studies have reported pathogenic species of borrelia related to those that cause tick-borne relapsing fever (rf), but unexpectedly suggesting their transmission by hard ticks, known vectors of borrelia burgdorferi sensu lato (b. burgdorferi s.l.) species, rather than by soft ticks. this study was designed to update the presence of b. burgdorferi s.l. species in ticks from several districts of mainland portugal, where ixodes ricinus had been previously described. tick ...201626976475
presence of host-seeking ixodes ricinus and their infection with borrelia burgdorferi sensu lato in the northern apennines, italy.host-seeking ticks were collected in the northern apennines, italy, by dragging at 35 sites, at altitudes ranging from 680 and 1670 m above sea level (asl), from april to november, in 2010 and 2011. ixodes ricinus (4431 larvae, 597 nymphs and 12 adults) and haemaphysalis punctata (11,209 larvae, 313 nymphs, and 25 adults) were the most abundant species, followed by haemaphysalis sulcata (20 larvae, five nymphs, and 13 adults), dermacentor marginatus (42 larvae and two adults) and ixodes hexagonu ...201626964552
evaluation of different nested pcrs for detection of anaplasma phagocytophilum in ruminants and ticks.anaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. currently, numerous pcr assays have been developed and used for the detection of a. phagocytophilum in various specimens. however, their performance varies. the aim of this study was to evaluate the performance of five nested pcr assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods ...201626911835
detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria.in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria.201626791781
characterization of spotted fever group rickettsiae in ticks from a city park of rome, italy.ticks are vectors and important reservoirs for microbial agents that cause disease in humans and animals. among these pathogens, the members of rickettsia species play an important role in public health.201526783214
molecular detection and identification of spotted fever group rickettsiae in ticks collected from the west bank, palestinian territories.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group (sfg) rickettsiae. although spotted fever is prevalent in the middle east, no reports for the presence of tick-borne pathogens are available or any studies on the epidemiology of this disease in the west bank. we aimed to identify the circulating hard tick vectors and genetically characterize sfg rickettsia species in ixodid ticks from the west bank-palestinian territories.201626771654
new foci of haemaphysalis punctata and dermacentor reticulatus in the netherlands.in 2014 haemaphysalis punctata was found in several locations on the mainland of the netherlands for the first time since 1897. in the same areas dermacentor reticulatus and ixodes ricinus were found. haemaphysalis punctata and d. reticulatus were tested for presence of babesia spp. and rickettsia spp. by pcr. babesia spp. and spotted fever rickettsiae were not detected in any of the collected h. punctata, while several d. reticulatus (6%) collected from the same areas were found to be positive ...201626726807
vectors of crimean congo hemorrhagic fever virus in iran.ticks are important vectors and reservoirs of crimean congo hemorrhagic fever (cchf) virus. human beings may be infected whenever the normal life cycle of the infected ticks on non-human vertebrate hosts is interrupted by the undesirable presence of humans in the cycle. a total of 26 species of argasid and ixodid ticks have been recorded in iran; including nine hyalomma, two rhipicephalus, two dermacentor, five haemaphysalis, two boophilus, one ixodes and two argas as well as three ornithodoros ...201526623426
a preliminary investigation on ticks (acari: ixodidae) infesting birds in kızılırmak delta, turkey.ticks are mandatory blood-feeding ectoparasites of mammals, birds, reptiles, and even amphibians. turkey has a rich bird fauna and is located on the main migration route for many birds. however, information on ticks infesting birds is very limited. in the present study, we aimed to determine ticks infesting birds in kızılırmak delta, turkey. in 2014 autumn bird migration season, a total of 7,452 birds belonging to 79 species, 52 genera, 35 families, and 14 orders were examined for tick infestati ...201626487249
genetic characterization and molecular survey of babesia bovis, babesia bigemina and babesia ovata in cattle, dairy cattle and yaks in china.babesiosis is an important haemoparasitic disease, caused by the infection and subsequent intra-erythrocytic multiplication of protozoa of the genus babesia that impacts the livestock industry and animal health. the distribution, epidemiology and genetic characterization of b. bigemina, b. bovis, and b. ovata in cattle in china as well as the prevalence of these protozoan agents were assessed.201526452623
molecular survey of tick-borne pathogens in ixodid ticks collected from hunted wild animals in tuscany, italy.to determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals.201526433656
a pcr-rflp assay targeting rps8 gene for the discrimination between bovine babesia and theileria species in china.bovine babesiosis and theileriosis is an important hemoprotozoal disease in cattles and yaks in tropical and subtropical regions leading to significant economic losses. in the field, the risk of co-infection between the bovine babesia and theileria species is very high. thus, it is necessary to develop a simple, accurate, rapid and cost-effective method for large-scale epidemic investigation, in particular for the detection of co-infection in field.201526382041
a broad-range survey of ticks from livestock in northern xinjiang: changes in tick distribution and the isolation of borrelia burgdorferi sensu stricto.borreliosis is highly prevalent in xinjiang uygur autonomous region, china. however, little is known about the presence of borrelia pathogens in tick species in this region, in addition borrelia pathogens have not been isolated from domestic animals.201526337627
climate suitability for european ticks: assessing species distribution models against null models and projection under ar5 climate.there is increasing evidence that the geographic distribution of tick species is changing. whilst correlative species distribution models (sdms) have been used to predict areas that are potentially suitable for ticks, models have often been assessed without due consideration for spatial patterns in the data that may inflate the influence of predictor variables on species distributions. this study used null models to rigorously evaluate the role of climate and the potential for climate change to ...201526310856
patterns of tick infestation and their borrelia burgdorferi s.l. infection in wild birds in portugal.wild birds may act as reservoirs for zoonotic pathogens and may be mechanical carriers of pathogen infected vector ticks through long distances during migration. the aim of this study was to assess tick infestation patterns in birds in portugal and the prevalence of tick infection by borrelia burgdorferi s.l. using pcr techniques. seven tick species were collected from birds including haemaphysalis punctata, hyalomma spp., ixodes acuminatus, ixodes arboricola, ixodes frontalis, ixodes ricinus an ...201526159798
detection of lyme disease and q fever agents in wild rodents in central italy.the maintenance of tick-borne disease agents in the environment strictly depends on the relationship between tick vectors and their hosts, which act as reservoirs for these pathogens. a pilot study aimed to investigate wild rodents as reservoirs for zoonotic tick-borne pathogens (borrelia burgdorferi sensu lato (s.l.), coxiella burnetii, francisella tularensis, and anaplasma phagocytophilum) was carried out in an area of gran sasso e monti della laga national park (abruzzi region, central italy) ...201526134933
anaplasma infection in ticks, livestock and human in ghaemshahr, mazandaran province, iran.anaplasmosis is an important issue for animal breeders in terms of economic losses as well as a health concern to human. ticks are considered as the main vector of this disease. lack of documented information about anaplasma species in iran was the scope of this study to determine the population of ticks and the presence of anaplasma in ticks, domestic ruminants and also human beings in northern iran.201426114134
detection of tick-borne anaplasma bovis, anaplasma phagocytophilum and anaplasma centrale in spain.the genus anaplasma (rickettsiales: anaplasmataceae) includes species of medical and veterinary importance. the presence of anaplasma spp. in ticks from birds, as well as in haemaphysalis punctata (ixodida: ixodidae) specimens collected from cattle and vegetation in northern spain was investigated. a total of 336 ticks from birds [174 ixodes frontalis (ixodida: ixodidae), 108 h. punctata, 34 hyalomma marginatum (ixodida: ixodidae), 17 ixodes ricinus (ixodida: ixodidae) and three ixodes spp.], an ...201526111122
[distribution of tick species spread in kütahya vicinity].this study was performed to determine the tick species infested to the cattle and small ruminants in kütahya province and their seasonal activity and prevalence.201526081885
ticks on passerines from the archipelago of the azores as hosts of borreliae and rickettsiae.we examined the presence of borreliae and rickettsiae bacteria in ticks from wild passerine birds on three islands of the archipelago of the azores, the westernmost region of palearctic. a total of 266 birds belonging to eight species from seven families were examined on são miguel, santa maria and graciosa islands in 2013. ticks collected from these birds consisted of 55 ixodes frontalis (22 larvae, 32 nymphs, 1 adult female) and 16 haemaphysalis punctata nymphs. turdus merula and erithacus rub ...201526013915
the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii.q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ...201525978383
a survey of ticks (acari: ixodida) infesting some wild animals from sivas, turkey.in order to determine the species composition of infesting ticks, between 2011 and 2012 a total of 1118 wild animals were captured from various regions of zara, sivas province, turkey. a total of 138 ticks were obtained from the 58 host animals. ticks were identified as dermacentor marginatus (sulzer), haemaphysalis erinacei taurica pospelova-shtrom, haemaphysalis parva (neumann), haemaphysalis punctata canestrini and fanzago, haemaphysalis sulcata canestrini and fanzago, hyalomma marginatum koc ...201525784071
hard tick species of livestock and their bioecology in golestan province, north of iran.a survey on tick species composition was carried out in golestan province iran during year 2010-2011.the aim was to determine tick species parasitizing domestic ruminants and their seasonal population dynamics.201325629071
novel candidatus rickettsia species detected in nostril tick from human, gabon, 2014.we report the identification of a nymphal nostril tick (amblyomma sp.) from a national park visitor in gabon and subsequent molecular detection and characterization of tickborne bacteria. our findings provide evidence of a potentially new rickettsia sp. circulating in africa and indicate that tick bites may pose a risk to persons visiting parks in the region.201525625886
tick-borne pathogens and the vector potential of ticks in china.ticks, as obligate blood-sucking ectoparasites, attack a broad range of vertebrates and transmit a great diversity of pathogenic microorganisms. they are considered second only to mosquitoes as vectors of human disease, and the most important vector of pathogens of domestic and wild animals. of the 117 described species in the chinese tick fauna, 60 are known to transmit one or more diseases: 36 species isolated within china and 24 species isolated outside china. moreover, 38 of these species ca ...201525586007
[taxonomic status of the burana virus (burv) (bunyaviridae, nairovirus, tamdy group) isolated from the ticks haemaphysalis punctata canestrini et fanzago, 1877 and haem. concinna koch, 1844 (ixodidae, haemaphysalinae) in kyrgyzstan].complete genome sequence of the burana virus (burv) was determined using the next-generation sequencing approach (id genbank kf801651). the prototype strain of burv leiv-krg760 was originally isolated from the ticks haemaphysalis punctata canestrini et fanzago, 1877 (ixodidae, haemaphysalinae), collected from cows in tokmak wildlife sanctuary, eastern part of the chu valley (43 degrees 10' n, 74 degrees 40' e) near burana village, kirgizia, in april 1971. molecular genetics and phylogenetic anal ...201625549462
theileria sp. ot3 and other tick-borne pathogens in sheep and ticks in italy: molecular characterization and phylogeny.pcr reverse line blot (rlb) hybridization and sequencing were used to determine the dynamics of infection with tick-borne pathogens in one hundred apparently healthy sheep in italy. blood samples were tested once prior to the onset of the grazing season (june 2010) and once after the end of the grazing season (august 2010). ticks collected from sheep and from the vegetation were also tested by pcr/rlb. before grazing, 56% of the sheep harbored several tick-borne pathogens: anaplasma ovis was the ...201525448422
vector competence of the tick ixodes sinensis (acari: ixodidae) for rickettsia monacensis.cases of mediterranean spotted fever like rickettsioses, caused by rickettsia monacensis, have become more common in the last 10 years. in china, natural infection of r. monacensis in various tick species has been confirmed but the vector(s) of r. monacensis have not been recorded.201425406413
phytotherapeutic activity of euphorbia cyparissias extracts on ixodidae (acari) female ticks.given its numerous biologically active components, euphorbiaceae has been found to be a large plant family and polyvalent with quite interesting therapeutic activity that can be studied.201425392580
the first german map of georeferenced ixodid tick locations.georeferenced locations of ixodid ticks are required to depict the observed distribution of species. further, they are used as input data for species distribution models also known as niche models. the latter were applied to describe current and future (projected) tick distributions. beside model assumptions and selected climate parameters, the number of georeferenced tick locations available as a digital dataset is of fundamental importance for the reliability of such models. for germany, howev ...201425301245
crimean-congo hemorrhagic fever virus clades v and vi (europe 1 and 2) in ticks in kosovo, 2012.despite being a small country, kosovo represents one of the few foci of crimean-congo hemorrhagic fever (cchf) in europe. the distribution of kosovar tick vectors and the evolution of cchf virus in ticks are both as yet unknown. a better description of the extent and the genetic diversity of cchfv in ticks from endemic settings is essential, in order to be controlled. we investigated the 2012 distribution of kosovar ticks alongside the prevalence and the phylogeography of tick-derived cchfv. hya ...201425255381
prevalence and phylogenetic analysis of tick-borne encephalitis virus (tbev) in field-collected ticks (ixodes ricinus) in southern switzerland.tick-borne encephalitis is the most common tick-borne viral infection in europe with 3,000 human cases reported each year. in western europe, the castor bean tick, ixodes ricinus, is the principal vector of the tick-borne encephalitis virus (tbev). tbev appears to be spreading geographically and was recently detected for the first time in canton valais in the southern part of switzerland. the purpose of the present study was to survey the i. ricinus tick populations of canton valais for tbev.201425245773
re-emergence of bovine piroplasmosis in hungary: has the etiological role of babesia divergens been taken over by b. major and theileria buffeli?the prevalence of bovine babesiosis caused by babesia divergens has been declining during the past decades in northeastern hungary, and no cases have been observed since 2008. infections of cattle with b. major and theileria buffeli were hitherto reported in southern and western europe. in other parts of the globe, there is evidence of emergence and a growing clinical importance of t. buffeli and closely related genotypes of the t. orientalis complex.201425212410
survey on cattle ticks in nur, north of iran.to survey the prevalence of cattle ticks in nur county and prepare a list of tick fauna in this district.201425182439
population-based passive tick surveillance and detection of expanding foci of blacklegged ticks ixodes scapularis and the lyme disease agent borrelia burgdorferi in ontario, canada.we identified ticks submitted by the public from 2008 through 2012 in ontario, canada, and tested blacklegged ticks ixodes scapularis for borrelia burgdorferi and anaplasma phagocytophilum. among the 18 species of ticks identified, i. scapularis, dermacentor variabilis, ixodes cookei and amblyomma americanum represented 98.1% of the 14,369 ticks submitted. rates of blacklegged tick submission per 100,000 population were highest in ontario's eastern region; d. variabilis in central west and easte ...201425171252
identification of tick-borne pathogens in ticks feeding on humans in turkey.the importance of tick-borne diseases is increasing all over the world, including turkey. the tick-borne disease outbreaks reported in recent years and the abundance of tick species and the existence of suitable habitats increase the importance of studies related to the epidemiology of ticks and tick-borne pathogens in turkey. the aim of this study was to investigate the presence of and to determine the infection rates of some tick-borne pathogens, including babesia spp., borrelia burgdorferi se ...201425101999
ticks infesting humans in italy and associated pathogens.ticks may transmit a large variety of pathogens, which cause illnesses in animals and humans, commonly referred to as to tick-borne diseases (tbds). the incidence of human tbds in italy is underestimated because of poor surveillance and the scant amount of studies available.201425023709
[isolation of borrelia burgdorferi in ixodes from four counties, in north xinjiang].to identify ticks and determine the borrelia (b.) burgdorferi genotype from four counties of northern xinjiang.201424831623
habitat and occurrence of ixodid ticks in the liguria region, northwest italy.questing ticks were collected during monthly dragging sessions (march-august 2011) in three provinces of the liguria region, north-western italy, to evaluate the species occurrence, spatial distribution and relative abundance. a total of 1,464 specimens were collected in 94 dragging sites. ixodes ricinus was the most abundant species (81.3 % of collected ticks), followed by haemaphysalis punctata (10.9 %), dermacentor marginatus (5.5 %), ixodes frontalis (1.3 %), and rhipicephalus spp. (0.9 %). ...201424682615
gene cloning, expression and immunogenicity of the protective antigen subolesin in dermacentor silvarum.subolesin (4d8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. to date, no protective antigens have been characterized nor tested as candidate vaccines against dermacentor silvarum bites and transmission of associated pathogens. in this study, we cloned the open reading frame (orf) of d. silvarum 4d8 cdna (ds4d8), which consisted of 498 bp encoding 165 amino acid residu ...201424623890
seasonal dynamics of rhipicephalus rossicus attacking domestic dogs from the steppic region of southeastern romania.danube delta biosphere reserve is one of the most interesting regions in europe from an epidemiological point of view due to its great biodiversity, local climatic conditions and various types of habitats. moreover, there is no data regarding the ectoparasite communities of dogs from this area. in this frame, the aims of our study were to establish the tick communities parasitizing dogs and to provide new data regarding seasonal dynamics of a neglected tick species, rhipicephalus rossicus.201424612483
do the ticks of birds at an important migratory hotspot reflect the seasonal dynamics of ixodes ricinus at the migration initiation site? a case study in the danube delta.migratory birds play important roles as distributors of ticks within and between continents. in the old world, the most important migratory route of birds links asia, europe and africa. during their migration, birds use various stopover sites, where they feed and rest and where ticks may attach or detach, creating new natural foci for vector-borne diseases. danube delta is one of the most important migration hotspots and so far no studies were focused on ticks of migratory birds herein. the aim ...201424586732
assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex dna bead-based suspension array.piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. the purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the basque country (nort ...201324499621
a survey of ixodid tick species and molecular identification of tick-borne pathogens.this study was undertaken in two different climatic areas of turkey to determine the presence of tick-borne pathogens of medical and veterinary importance. the ticks were removed from humans, pooled according to species and developmental stages, and analyzed by pcr, reverse line blot (rlb) and sequencing. of the 2333 removed ticks from 10 species, 1238 (53.06%) were obtained from the arid cold zone, and the remaining 1095 (46.93%) were obtained from the humid zone. the removed ticks were identif ...201424424312
detection of naturally infected vector ticks (acari: ixodidae) by different species of babesia and theileria agents from three different enzootic parts of iran.diagnostic study of vector ticks for different pathogens transmitted specifically have been done by iranian old scientists working on the basis of biological transmission of pathogens. in this study we decided to confirm natural infection of different collected ticks from three different provinces of iran.201324409442
pcr-based detection of babesia spp. infection in collected ticks from cattle in west and north-west of iran.babesiosis is a haemoparasitic disease of domestic and wild animals caused by species of the genus babesia. babesia bigemina, b. bovis and b. divergens are known to be pathogenic in cattle. the disease is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. ixodid ticks are vectors in the transmission of babesiosis. the classic presentation is a febrile syndrome with apparent anemia and hemoglobinuria. thi ...201324409438
sensitivity to permethrin in a dermacentor reticulatus population from eastern poland in laboratory study.the action of chemical compounds on the palaearctic tick d. reticulatus (fabricius) (acari: amblyomminae) has been poorly investigated so far. therefore, the effects of application of permethrin on engorged d. reticulatus females have been assessed, and the survival rate for the different developmental stages of the tick species in its non-parasitic phase of the life cycle was determined upon application of the pyrethroid.201424405550
molecular and serological evidence for the circulation of the tick symbiont midichloria (rickettsiales: midichloriaceae) in different mammalian species.the midichloriaceae is a novel family of the order rickettsiales, that encompasses intracellular bacteria associated with hard ticks (ixodidae) and other arthropods. the most intensively investigated member of this family is midichloria mitochondrii, a symbiotic bacterium of the sheep tick ixodes ricinus, characterized by the capacity of multiplying inside the mitochondria. a recent study suggested that these bacteria might be inoculated into the human host during the tick bite. the purpose of t ...201324330701
rps8--a new informative dna marker for phylogeny of babesia and theileria parasites in china.piroplasmosis is a serious debilitating and sometimes fatal disease. phylogenetic relationships within piroplasmida are complex and remain unclear. we compared the intron-exon structure and dna sequences of the rps8 gene from babesia and theileria spp. isolates in china. similar to 18s rdna, the 40s ribosomal protein s8 gene, rps8, including both coding and non-coding regions is a useful and novel genetic marker for defining species boundaries and for inferring phylogenies because it tends to ha ...201324244571
prevalence of tick-borne encephalitis virus in ixodid ticks collected from the republic of korea during 2011-2012.in this study, we demonstrated that tbev-infected ticks have been distributed in the rok, combined with our previous results. these results suggest that tbev may exist in the rok, and h. longicornis, h. flava, and i. nipponensis may be potential vectors of tbev. in addition, these results emphasize the need for further epidemiological research of tbev.201224159517
seasonal dynamics of tick species in an urban park of rome.regular collections were obtained in the natural reserve of the insugherata of rome during 2011 in order to obtain the tick species composition and the respective seasonal dynamics of the area. a total of 325 ticks was collected in selected sites by means of drag sampling. among the identified species, rhipicephalus turanicus was the most abundant (72.3%), followed by ixodes ricinus (19.7%), dermacentor marginatus (6.5%), haemaphysalis punctata (1.2%), and rhipicephalus bursa (0.3%). r. turanicu ...201324140501
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
prevalence of ixodid tick infestation of sheep in the arasbaran region of iran.this survey was carried out in sheep herds of arasbaran region in northwest of iran. the aims of this survey were assessment of occurrence hard ticks on sheep in this region and speciation and identification of detached ticks from sheep. in addition determination of tick distribution on sheep body surface was studied. in this study 330 sheep were examined and ticks from infested animals were collected. in parasitological laboratory speciation of ticks were done by stereomicroscope and using of t ...201224082534
detection and genetic characterization of tick-borne encephalitis virus (tbev) derived from ticks removed from red foxes (vulpes vulpes) and isolated from spleen samples of red deer (cervus elaphus) in croatia.tick-borne encephalitis (tbe) is a growing public health concern in central and northern european countries. even though tbe is a notifiable disease in croatia, there is a significant lack of information in regard to vector tick identification, distribution as well as tbe virus prevalence in ticks or animals. the aim of our study was to identify and to investigate the viral prevalence of tbe virus in ticks removed from red fox (vulpes vulpes) carcasses hunted in endemic areas in northern croatia ...201424035586
mediterranean spotted fever in southeastern romania.although cases of mediterranean spotted fever (msf) have been reported for decades in southeastern romania, there are few published data. we retrospectively studied 339 patients, diagnosed with msf at the national institute of infectious diseases "prof. dr. matei bals" between 2000 and 2011, in order to raise awareness about msf in certain regions of romania. according to the raoult diagnostic criteria 171 (50.4%) had a score >25 points. mean age was 52.5 years. one hundred and fifty-five (90.6% ...201324024190
spotted fever group rickettsiae in dermacentor reticulatus and haemaphysalis punctata ticks in the uk.spotted fever group (sfg) rickettsiae have recently been identified for the first time in uk ticks. this included the findings of rickettsia helvetica in ixodes ricinus and rickettsia raoultii in dermacentor reticulatus. this paper further investigates the occurrence of sfg rickettsiae in additional geographically distinct populations of d. reticulatus, and for the first time, investigates the occurrence of sfg rickettsiae in uk populations of haemaphysalis punctata ticks.201323870197
spatial disaggregation of tick occurrence and ecology at a local scale as a preliminary step for spatial surveillance of tick-borne diseases: general framework and health implications in belgium.the incidence of tick-borne diseases is increasing in europe. sub national information on tick distribution, ecology and vector status is often lacking. however, precise location of infection risk can lead to better targeted prevention measures, surveillance and control.201323800283
spotted fever group rickettsiae in questing ticks, central spain. 023763913
complete mitochondrial genome sequence data provides genetic evidence that the brown dog tick rhipicephalus sanguineus (acari: ixodidae) represents a species complex.ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, and cause a variety of human and animal diseases worldwide. in the present study, we sequenced the complete mitochondrial (mt) genome of rhipicephalus sanguineus from china (rsc) and compared with that of r. sanguineus from usa (rsu). nucleotide sequence difference in the full mt genome was 11.23% between rsc and rsu. for the 13 protein-coding genes, compari ...201323630448
ticks of poland. review of contemporary issues and latest research.the paper presents current knowledge of ticks occurring in poland, their medical importance, and a review of recent studies implemented in the polish research centres on ticks and their significance in the epidemiology of transmissible diseases. in the polish fauna there are 19 species of ticks (ixodida) recognized as existing permanently in our country: argas reflexus, argas polonicus, carios vespertilionis, ixodes trianguliceps, ixodes arboricola, ixodes crenulatus, ixodes hexagonus, ixodes li ...201223444797
hard ticks (acari, ixodidae) of croatia.the present paper is based on original and literature data. in croatia the first studies on the occurrence of ixodid species were made about 80 years ago. the number of tick species recorded in croatia considerably increased during the 1950s, 60s, 70s and 80s of the past century. a total of 21 species of hard tick belonging to 5 genera have been recorded in croatia. ixodes is the best represented genus, with seven species recorded. haemaphysalis is represented by six species, followed by rhipice ...201223372407
characterization of the bhanja serogroup viruses (bunyaviridae): a novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses.bhanja virus (bhav) and its antigenically close relatives forecariah virus (forv), kismayo virus (kisv), and palma virus (palv) are thought to be members of the family bunyaviridae, but they have not been assigned to a genus or species. despite their broad geographical distribution and reports that bhav causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemi ...201323325688
characterization of the uukuniemi virus group (phlebovirus: bunyaviridae): evidence for seven distinct species.evolutionary insights into the phleboviruses are limited because of an imprecise classification scheme based on partial nucleotide sequences and scattered antigenic relationships. in this report, the serologic and phylogenetic relationships of the uukuniemi group viruses and their relationships with other recently characterized tick-borne phleboviruses are described using full-length genome sequences. we propose that the viruses currently included in the uukuniemi virus group be assigned to five ...201323283959
abiotic and biotic factors associated with tick population dynamics on a mammalian host: ixodes hexagonus infesting otters, lutra lutra.the eurasian otter, lutra lutra, hosts several parasites with zoonotic potential. as this semiaquatic mammal has large ranges across terrestrial, freshwater and marine habitats, it has the capacity for wide dispersion of pathogens. despite this, parasites of otters have received relatively little attention. here, we examine their ectoparasite load and assess whether this is influenced by abiotic or biotic variables. climatic phenomena such as the north atlantic oscillation (nao) affect weather c ...201223071736
association of environmental traits with the geographic ranges of ticks (acari: ixodidae) of medical and veterinary importance in the western palearctic. a digital data set.we compiled information on the distribution of ticks in the western palearctic (11°w, 45°e; 29°n, 71°n), published during 1970-2010. the literature search was filtered by the tick's species name and an unambiguous reference to the point of capture. records from some curated collections were included. we focused on tick species of importance to human and animal health, in particular: ixodes ricinus, dermacentor marginatus, d. reticulatus, haemaphysalis punctata, h. sulcata, hyalomma marginatum, h ...201222843316
analysis of pathogen co-occurrence in host-seeking adult hard ticks from serbia.past studies in serbia have reported concurrent infections of ixodes ricinus ticks with borrelia burgdorferi sensu lato genospecies, anaplasma phagocytophilum and francisella tularensis. as a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in serbia. five tick species were identified (dermacentor marginatus, d. reticulatus, haemaphysalis punctata, h. concinna and i. ricinus) and analyzed for the presence of seven pathogen ...201322773070
role of birds in dispersal of etiologic agents of tick-borne zoonoses, spain, 2009.we amplified gene sequences from anaplasma phagocytophilum, borrelia garinii, b. valaisiana, b. turdi, rickettsia monacensis, r. helvetica, r. sibirica sibirica, and rickettsia spp. (including candidatus rickettsia vini) in ticks removed from birds in spain. the findings support the role of passerine birds as possible dispersers of these tick-borne pathogens.201222709801
diversity and seasonal patterns of ticks parasitizing wild birds in western portugal.the diversity and abundance of questing ticks and ticks parasitizing birds was assessed during 1 year in two recreational forests in western portugal, a suburban forest and an enclosed game area. the aim of this study was to assess the distribution and seasonality of tick species and to understand the role of bird species as hosts for ticks. ixodes ricinus was the most abundant questing tick collected in the enclosed game area, whereas in the suburban forest, only three ticks were collected by b ...201222669280
ixodes ricinus is the dominant questing tick in forest habitats in romania: the results from a countrywide dragging campaign.in 2010 and 2011, questing ticks were collected from 188 forested locations in all the 41 counties of romania using the dragging method. the total of 13,771 ticks collected belonged to eleven species: ixodes ricinus (86.9 %), dermacentor marginatus (9.5 %), haemaphysalis punctata (2.6 %), h. concinna (0.6 %), h. sulcata (0.3 %), h. parva (0.1 %), hyalomma marginatum (0.02 %), d. reticulatus (0.02 %), i. crenulatus (0.007 %), i. hexagonus (0.007 %) and i. laguri (0.007 %). ixodes ricinus was pres ...201222547023
synopsis of the hard ticks (acari: ixodidae) of romania with update on host associations and geographical distribution.the current paper is a synoptic review of the distribution and host associations of the 25 species of hard tick fauna (family ixodidae) in romania. in addition to a full literature survey, original data is presented, based on eight years of occasional or targeted sample collection. the literature data on geographical distribution was transposed digitally to the decimal degree coordinate system. for each species, an updated distribution map is given together with all historical data and new host ...201222544174
first molecular detection of theileria ovis in rhipicephalus sanguineus tick in iran.to determine tick infestation of domestic ruminants and their infection to ovine theileriosis in northern iran.201222182639
spotted fever group rickettsiae in ticks collected from wild animals in israel.we report molecular evidence for the presence of spotted fever group rickettsiae (sfgr) in ticks collected from roe deer, addax, red foxes, and wild boars in israel. rickettsia aeschlimannii was detected in hyalomma marginatum and hyalomma detritum while rickettsia massiliae was present in rhipicephalus turanicus ticks. furthermore, a novel uncultured sfgr was detected in haemaphysalis adleri and haemaphysalis parva ticks from golden jackals. the pathogenicity of the novel sfgr for humans is unk ...201122049050
blood parasites of sheep in the netherlands. i. anaplasma mesaeterum sp.n. (rickettsiales, anaplasmataceae).summary on two occasions an anaplasm was isolated from sheep on the dutch island of ameland. the organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. treatment with oxytetracycline was effective. its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. this anaplasm differs from anaplasma ovis in that less than 30% ...197922040472
blood parasites of sheep in the netherlands. ii. babesia motasi (sporozoa, babesiidae).summary a large babesia species occurs in sheep on the north sea islands of the netherlands. the tick haemaphysalis punctata is a vector. its pathogenicity appears to be low. it is morphologically similar to a turkish strain, considered to be b. motasi, which is also transmitted by haemaphysalis ticks. it differs from the turkish parasite serologically as well as in cross-immunity tests and in not being effective to goats. there may be a group of morphologically similar parasites with ser ...198022039853
First transcriptome of the testis-vas deferens-male accessory gland and proteome of the spermatophore from Dermacentor variabilis (Acari: Ixodidae).Ticks are important vectors of numerous human diseases and animal diseases. Feeding stimulates spermatogenesis, mating and insemination of male factors that trigger female reproduction. The physiology of male reproduction and its regulation of female development are essentially a black box. Several transcriptomes have catalogued expression of tick genes in the salivary glands, synganglion and midgut but no comprehensive investigation has addressed male reproduction and mating. Consequently, a ne ...201121949745
prevalence of ectoparasitic arthropods on wild animals and cattle in the las merindades area (burgos, spain).this paper reports the prevalence of ectoparasitic arthropods in sampled groups of wild (n = 128; 16 species) and domestic (n = 69; 3 species) animals in the las merindades area of the province of burgos, spain. the study revealed that wild animals were more infested and with a wider variety of ectoparasites than domestic animals. the parasitic prevalence was 67% for wild animals and 48% for livestock. in this way, 39% of animals were infected by ticks. ixodes ricinus and ixodes hexagonus were t ...201121894267
spotted fever group rickettsiae in ticks in cyprus.in two surveys conducted from march 1999 to march 2001 and from january 2004 to december 2006, a total of 3,950 ticks (belonging to ten different species) were collected from seven domestic and wild animals (goat, sheep, cattle, dog, fox, hare, and mouflon) from different localities throughout cyprus. in order to establish their infection rate with spotted fever rickettsiae (sfg), ticks were pooled and tested by polymerase chain reaction targeting glta and ompa genes, followed by sequencing anal ...201121833539
evaluation of surrogate animal models of melioidosis.burkholderia pseudomallei is the gram-negative bacterial pathogen responsible for the disease melioidosis. b. pseudomallei establishes disease in susceptible individuals through multiple routes of infection, all of which may proceed to a septicemic disease associated with a high mortality rate. b. pseudomallei opportunistically infects humans and a wide range of animals directly from the environment, and modeling of experimental melioidosis has been conducted in numerous biologically relevant mo ...201021772830
coinfections of rickettsia slovaca and rickettsia helvetica with borrelia lusitaniae in ticks collected in a safari park, portugal.borrelia and rickettsia bacteria are the most important tick-borne agents causing disease in portugal. identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. a total of 677 questing ticks including dermacentor marginatus, rhipicephalus sanguineus, ixodes ricinus, hyalomma lusitanicum, h. marginatum, and haemaphysalis punctata we ...201021771525
the hard-tick fauna of mainland portugal (acari: ixodidae): an update on geographical distribution and known associations with hosts and pathogens.this work is an updated revision of the available information on portuguese ixodid tick species. it includes data on tick biology, ecology, taxonomy and host/pathogen-associations. the current list of portuguese ixodid ticks comprises twenty species: dermacentor marginatus (sulzer, 1776), dermacentor reticulatus (fabricius, 1794), haemaphysalis hispanica gil collado, 1938, haemaphysalis inermis birula, 1895, haemaphysalis punctata canestrini & fanzago, 1878, hyalomma lusitanicum koch, 1844, hyal ...201121452063
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