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spotted fever group rickettsiae associated with ixodid ticks in wild environment in southern italy.ixodidae ticks are vectors and reservoirs of several species of rickettsiae, and tick-borne rickettsioses are reported worldwide. this study was aimed to verify the distribution of spotted fever group rickettsiae associated with ticks in a wild environment, the national park of gargano, where there is proximity between wild and domestic animals, and which is within an endemic area for rickettsiosis. ticks were collected from animals or vegetation, morphologically identified and tested by a pcr t ...201729047217
tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern portugal.ticks are vectors of many human and animal pathogens. the aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern portugal. a total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). overall, the analysis of dna extracts prepared from ticks collected from hosts of 11 different species in the distric ...201728958791
molecular detection, infection rate and vectors of theileria lestoquardi in goats from west azerbaijan province, iran.this study was aimed to determine the infection rate and vectors of theileria lestoquardi in goats from west azerbaijan province, iran. a total of 400 blood samples were collected from 40 randomly selected flocks in the study area from june to september, 2014. out of 400 blood samples examined using microscopic examination, a number of 14 goats (3.50%) were positive for theileria spp., whereas 25 goats (6.25%) yielded a specific t. lestoquardi ssu-rrna fragment (235 bp). the prevalence of theile ...201728785390
one particular anaplasma phagocytophilum ecotype infects cattle in the camargue, france.anaplasma phagocytophilum is a zoonotic tick-borne pathogen responsible for granulocytic anaplasmosis, a mild to a severe febrile disease that affects man and several animal species, including cows and horses. in europe, i. ricinus is the only proven vector for this pathogen, but studies suggest that other tick genera and species could be involved in its transmission. our objective was to assess the presence and genetic diversity of a. phagocytophilum in domestic animals and different tick speci ...201728764743
combination of rt-pcr and proteomics for the identification of crimean-congo hemorrhagic fever virus in ticks.crimean-congo hemorrhagic fever (cchf) is an emerging tick-borne zoonotic disease caused by the cchf virus (cchfv). in this study, an experimental approach combining rt-pcr and proteomics was used for the identification and characterization of cchfv in 106 ticks from 7 species that were collected from small ruminants in greece. the methodological approach included an initial screening for cchfv by rt-pcr followed by proteomics analysis of positive and control negative tick samples. this novel ap ...201728736753
diversity and distribution of ticks from domestic ruminants in lebanon.ticks (acari: ixodidae) are ectoparasites infesting livestock in every geographic area in the world and they are vectors of several viral, bacterial, and protozoan pathogens to animals and humans worldwide. a deep knowledge of the geographical distribution of these arthropods would have a key role in the control of tick-borne diseases. few data are available about tick presence in domestic ruminants in lebanon. the study aimed at providing an analysis of tick presence and distribution in lebanon ...201728675252
partial characterization of tick-borne encephalitis virus isolates from ticks of southern ukraine.tick-borne encephalitis (tbe) is the most common tick-borne viral infection in eurasia; thousands of human cases are annually reported from several european countries. several tick species are vectors of the tick-borne encephalitis virus (tbev), while tbe appears to be spreading from the eurasian continent westward to europe. fifteen study sites were chosen from five territories of southern ukraine, including odessa, mykolaiv, kherson oblast, the autonomous republic of crimea, and sevastopol. ti ...201728654319
rickettsia spp. in dermacentor marginatus ticks: analysis of the host-vector-pathogen interactions in a northern mediterranean area.in this work, we have analyzed the key features of rickettsia spp. maintenance in dermacentor marginatus ticks in four regions of the northern mediterranean area, where extensive investigations are currently lacking. for this purpose, 380 adult d. marginatus ticks were collected from vegetation and wild ungulates during a 3-year study. a host-vector-pathogen interaction approach was applied to investigate the key components of the disease systems and evaluate their respective weight in the inten ...201728500468
a retrospective study of the characterization of rickettsia species in ticks collected from humans.rickettsiae (family rickettsiaceae, order rickettsiales) are obligate intracellular bacteria transmitted by arthropod vectors. several rickettsia species causing vector-borne rickettsioses belong to the spotted fever group (sfg). traditionally, rickettsia conorii has been considered as the main etiologic agent of mediterranean spotted fever. however, the molecular characterization of rickettsiae allowed identifying other species involved in spotted fever in the mediterranean region. in this stud ...201728457821
crimean-congo hemorrhagic fever virus in ticks in turkey: a broad range tick surveillance study.in turkey, crimean-congo hemorrhagic fever (cchf) outbreaks started in northern regions in 2002. human cases still continue to increase and the disease spreads in many other provinces of turkey. the ecological role of the vector tick species occurring in turkey is not certain exactly. therefore, we planned a broad range tick study in three different ecological and geographical areas extending from the west black sea regions down to the central anatolia. the aim of this study was to determine and ...201728433738
mesocarnivores and macroparasites: altitude and land use predict the ticks occurring on red foxes (vulpes vulpes).the red fox vulpes vulpes is the most common mesocarnivore in europe and with a wide geographical distribution and a high density in most terrestrial habitats of the continent. it is fast urbanising species, which can harbor high numbers of different tick species, depending on the region. here we present the results of a large-scale study, trying to disentangle the intricate relationship between environmental factors and the species composition of ectoparasites in red foxes. the samples were col ...201728381228
molecular characterization of babesia and theileria species in ticks collected in the outskirt of monte romano, lazio region, central italy.in 2012-2013, an investigation was carried out in the viterbo province, lazio region, on ticks and tick-borne apicomplexan protozoa of the babesia and theileria genera. this followed the reporting of high density of ticks by soldiers operating in a military shooting range, and the signaling by owners and local veterinary authorities of several cases of babesiosis among cattle.201728361802
molecular evidence of rickettsia spp. in ixodid ticks and rodents in suburban, natural and rural habitats in slovakia.natural foci of tick-borne spotted fever group (sfg) rickettsiae of public health concern have been found in slovakia, but the role of rodents in their circulation is unclear. ticks (ixodes ricinus, ixodes trianguliceps, dermacentor marginatus, dermacentor reticulatus, haemaphysalis concinna and haemaphysalis inermis) and tissues of rodents (apodemus flavicollis, apodemus sylvaticus, myodes glareolus, microtus arvalis, microtus subterraneus and micromys minutus) were examined for the presence of ...201728340608
a comparative test of ixodid tick identification by a network of european researchers.this study reports the results of a comparative test of identification of ticks occurring in western europe and northern africa. a total of 14 laboratories were voluntarily enrolled in the test. each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: dermacentor marginatus, d. reticulatus, haemaphysalis punctata, hyalomma lusitanicum, hy. marginatum, ixodes ricinus, i. hexagonus, rhipicephalus annulatus, r. bursa, r. rossicus, and/or r. sanguineus s.l. tic ...201728320640
pyrosequencing based assessment of bacterial diversity in turkish rhipicephalus annulatus and dermacentor marginatus ticks (acari: ixodidae).ticks continue to be a threat to human and animal health in turkey, as they are considered important vectors of human and animal diseases. the objectives of this investigation are to characterize the microbial communities of two tick species, rhipicephalus annulatus and dermacenter marginatus, analyze patterns of co-occurrence among microbial taxa, identify and compare pathogens contributing human diseases, and determine whether avirulent symbionts could exclude human pathogens from tick communi ...201728111714
detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy.tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ...201728110915
tick-borne diseases in turkey: a review based on one health perspective.the importance of tick-borne diseases is increasing all over the world, including turkey. global warming, environmental and ecological changes and the existence of suitable habitats increase the impact of ticks and result in frequent emergence or re-emergence of tick-borne diseases (tbds) with zoonotic characteristics. in turkey, almost 19 tbds have been reported in animals and men, involving four protozoa (babesiosis, theileriosis, cytauxzoonosis, hepatozoonosis), one filarial nematode (acantho ...201627977689
pcr screening of tick-borne agents in sensitive conservation areas, southeast portugal.the southeast region of portugal, particularly the guadiana valley, is currently the reintroduction territory of lynx pardinus (iberian lynx), one of the most endangered felids in the world that is only found in the iberian peninsula. over the last century, populations have declined, placing l. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. among the aspects taken into account in the establishment of new populations is the sanitary status of the ...201727894847
first report of rickettsia raoultii and r. slovaca in melophagus ovinus, the sheep ked.melophagus ovinus (diptera: hippoboscidae), a hematophagous ectoparasite, is mainly found in europe, northwestern africa, and asia. this wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. herein, we investigated the presence of various rickettsia species in m. ovinus.201627884170
ticks (acari: ixodidae) infesting cattle and some other domestic and wild hosts on the french mediterranean island of corsica.corsica is a mountainous french island in the north-western mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. despite a favourable context for ticks and tick-borne diseases (tbds), the tick fauna of corsica has not systematically been investigated.201627842608
introduction and persistence of tularemia in bulgaria.outbreaks of the zoonotic disease tularemia occurred in north-east bulgaria in the 1960s. then came 30 years of epidemiological silence until new outbreaks occurred in west bulgaria in the 1990s. to investigate how bacterial strains of francisella tularensis causing tularemia in wildlife and humans in the 1960s and the 1990s were related, we explored their genetic diversity.201627790972
assessment of domestic goats as models for experimental and natural infection with the north american isolate of rickettsia slovaca.rickettsia slovaca is a tick-borne human pathogen that is associated with scalp eschars and neck lymphadenopathy known as tick-borne lymphadenopathy (tibola) or dermacentor-borne necrosis erythema and lymphadenopathy (debonel). originally, r. slovaca was described in eastern europe, but since recognition of its pathogenicity, human cases have been reported throughout europe. european vertebrate reservoirs of r. slovaca remain unknown, but feral swine and domestic goats have been found infected o ...201627741322
bacterial pathogens and endosymbionts in ticks.ticks collected from goats in northern greece were tested for the presence of tick-borne bacteria. among adult ticks, 37 (57.8%) were rhipicephalus bursa, 11 (17.2%) dermacentor marginatus, 10 (15.6%) ixodes ricinus, 3 (4.7%) rhipicephalus sanguineus sensu lato and 2 (3.1%) haemaphysalis parva; one (1.6%) rhipicephalus spp. tick was nymph. rickettsia monacensis, rickettsia massilae, anaplasma phagocytophilum and anaplasma platys were detected in i. ricinus and rh. bursa ticks. a variety of coxie ...201727686386
species diversity and spatial distribution of ixodid ticks on small ruminants in greece.a cross sectional field study on the distribution of ixodidae ticks was carried out over two consecutive tick seasons (2012 and 2013) in small ruminants (309 farms) located in mainland and five islands of greece. a total of 2108 ticks (1199 females, 908 males, and 1 nymph) were collected from sheep and goats. two species of rhipicephalus, two of ixodes, five of hyalomma, three of haemaphysalis, and one of dermacentor were detected. the species with the widest distribution were rhipicephalus sang ...201627655133
tick species, tick-borne pathogens and symbionts in an insular environment off the coast of western france.insular environments provide ideal natural conditions to study disease ecology, especially emerging diseases, due to clear differentiation between local and long-distance transmission. such environments are of particular interest regarding tick-borne pathogens (tbp), since animal exchange with the mainland (along with any ticks they carry) is limited, and because such locations could lie on migratory routes for birds carrying ticks. therefore both tick species and tbp may display different preva ...201627622976
seasonal dynamics of ixodid ticks on wild rabbits oryctolagus cuniculus (leporidae) from central spain.rabbit hemorrhagic disease and myxomatosis caused a decline in the rabbit population in the second half of the last century. despite some recovery, the risk of vector-borne disease is present and thus the importance of controlling vector populations. in the current study, we describe the ixodid tick fauna in wild rabbit in a natural reserve in ciudad real (central spain) during the course of two 3-year periods (2007-2009 and 2012-2014). of all the ticks collected on average 72.5 % were larvae, 2 ...201627411939
anaplasma marginale and theileria annulata in questing ticks from portugal.ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...201627394441
sarcoptes scabiei infestation does not alter the stability of ectoparasite communities.the host represents a heterogeneous ecosystem where multiple parasite species co-occur and interact with each other for space and resources. although these interactions may rule the features of an infracommunity and may shape the infracommunity response to external perturbations, the resilience of ectoparasite communities to new infestations remains poorly explored.201627370780
exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast italy.many vector-borne pathogens including viruses, bacteria, protozoa and nematodes occur in northeast italy, representing a potential threat to animal and human populations. little information is available on the circulation of the above vector-borne pathogens in dogs. this work aims to (i) assess exposure to and circulation of pathogens transmitted to dogs in northeast italy by ticks, sandflies, and mosquitoes, and (ii) drive blood donor screening at the newly established canine blood bank of the ...201627357128
spotted fever group rickettsiae in ticks of migratory birds in romania.birds are important hosts and dispersers of parasitic arthropods and vector-borne zoonotic pathogens. particularly migratory species may carry these parasites over long distances in short time periods. migratory hotspots present ideal conditions to get a snapshot of parasite and pathogen diversity of birds migrating between continents. the aim of this study was to investigate the presence and diversity of rickettsia spp. in ticks collected from birds at a migratory hot-spot in the danube delta, ...201627207258
molecular detection of spotted fever group rickettsiae in ticks removed from humans in turkey.a total of 229 attached hard ticks were collected from humans in yozgat province (turkey) in 2009. all ticks were individually examined for the presence of spotted fever group (sfg) rickettsiae using the polymerase chain reaction (pcr) targeting rickettsial citrate synthase (glta) and outer membrane protein (ompa) genes. rickettsial dna was detected in 24 (10.5%) tick samples belonging to dermacentor marginatus (sulzer) (9 males, 3 females), hyalomma marginatum koch (8 males, 2 females) and haem ...201627131413
tick-borne infections in human and animal population worldwide.the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...201527047089
perspectives on modelling the distribution of ticks for large areas: so far so good?this paper aims to illustrate the steps needed to produce reliable correlative modelling for arthropod vectors, when process-driven models are unavailable. we use ticks as examples because of the (re)emerging interest in the pathogens they transmit. we argue that many scientific publications on the topic focus on: (i) the use of explanatory variables that do not adequately describe tick habitats; (ii) the automatic removal of variables causing internal (statistical) problems in the models withou ...201627030357
the ecological niche of dermacentor marginatus in germany.the ixodid tick dermacentor marginatus (sulzer, 1776) is endemic throughout southern europe in the range of 33-51 (°) n latitude. in germany, however, d. marginatus was exclusively reported in the rhine valley and adjacent areas. its northern distribution limit near giessen is located at the coordinates 8.32 (°) e/50.65 (°) n. particularly with regard to the causative agents of rickettsioses, tularemia, and q fever, the observed locations as well as the potential distribution of the vector d. ma ...201626993325
novel spotted fever group rickettsiae in haemaphysalis qinghaiensis ticks from gansu, northwest china.rickettsia spp. are obligate intracellular bacteria and well known as transmitted by arthropods. these pathogens have a broad geographic distribution and a high degree of biological and clinical diversity. this study was conducted to determine the prevalence and molecular characterization of rickettsia spp. in ticks collected from gansu, where borrelia burgdorferi sensu lato and anaplasma phagocytophilum were previously reported in ticks and ruminants.201626968160
presence of host-seeking ixodes ricinus and their infection with borrelia burgdorferi sensu lato in the northern apennines, italy.host-seeking ticks were collected in the northern apennines, italy, by dragging at 35 sites, at altitudes ranging from 680 and 1670 m above sea level (asl), from april to november, in 2010 and 2011. ixodes ricinus (4431 larvae, 597 nymphs and 12 adults) and haemaphysalis punctata (11,209 larvae, 313 nymphs, and 25 adults) were the most abundant species, followed by haemaphysalis sulcata (20 larvae, five nymphs, and 13 adults), dermacentor marginatus (42 larvae and two adults) and ixodes hexagonu ...201626964552
evaluation of different nested pcrs for detection of anaplasma phagocytophilum in ruminants and ticks.anaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. currently, numerous pcr assays have been developed and used for the detection of a. phagocytophilum in various specimens. however, their performance varies. the aim of this study was to evaluate the performance of five nested pcr assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods ...201626911835
a global genomic characterization of nairoviruses identifies nine discrete genogroups with distinctive structural characteristics and host-vector associations.nairoviruses are primarily tick-borne bunyaviruses, some of which are known to cause mild-to-severe febrile illness in humans or livestock. we describe the genome sequences of 11 poorly characterized nairoviruses that have ecological associations with either birds (farallon, punta salinas, sapphire ii, zirqa, avalon, clo mor, taggert, and abu hammad viruses), rodents (qalyub and bandia viruses), or camels (dera ghazi khan virus). global phylogenetic analyses of proteins encoded in the l, m, and ...201626903607
candidatus coxiella massiliensis infection.bacteria genetically related to coxiella burnetii have been found in ticks. using molecular techniques, we detected coxiella-like bacteria, here named candidatus coxiella massiliensis, in skin biopsy samples and ticks removed from patients with an eschar. this organism may be a common agent of scalp eschar and neck lymphadenopathy after tick bite.201626811945
detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria.in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria.201626791781
characterization of spotted fever group rickettsiae in ticks from a city park of rome, italy.ticks are vectors and important reservoirs for microbial agents that cause disease in humans and animals. among these pathogens, the members of rickettsia species play an important role in public health.201526783214
isolation of a rickettsia slovaca-like agent from dermacentor variabilis ticks in vero cell culture.rickettsia slovaca is transmitted by dermacentor marginatus ticks, and is the causative agent of tick-borne lymphadenopathy and dermacentor-borne necrosis erythema lymphadenopathy throughout europe. it has not been found in new world ticks, nor have tick-borne lymphadenopathy or dermacentor-borne necrosis erythema lymphadenopathy been reported in humans in the americas. here we describe the isolation of a r. slovaca-like agent from d. variabilis nymphs from a colony of ticks derived from field c ...201626771652
molecular investigation of francisella-like endosymbiont in ticks and francisella tularensis in ixodid ticks and mosquitoes in turkey.this study was carried out to investigate the molecular prevalence of francisella-like endosymbionts (fles) and francisella tularensis in ticks (acari: ixodidae) and mosquitoes in turkey. genomic dna pools were constructed from a total of 1477 adult hard ticks of rhipicephalus (rh.) annulatus, rh. turanicus, rh. sanguineus, rh. bursa, haemaphysalis (hae.) parva, hae. sulcata, hyalomma marginatum marginatum, h. anatolicum anatolicum, h. anatolicum excavatum, h. detritum detritum, h. dromedarii, d ...201626741324
african swine fever virus transmission cycles in central europe: evaluation of wild boar-soft tick contacts through detection of antibodies against ornithodoros erraticus saliva antigen.african swine fever (asf) is one of the most complex viral diseases affecting both domestic and wild pigs. it is caused by asf virus (asfv), the only dna virus which can be efficiently transmitted by an arthropod vector, soft ticks of the genus ornithodoros. these ticks can be part of asfv-transmission cycles, and in europe, o. erraticus was shown to be responsible for long-term maintenance of asfv in spain and portugal. in 2014, the disease has been reintroduced into the european union, affecti ...201626728767
long term study of ixodid ticks feeding on red deer (cervus elaphus) in a meso-mediterranean climate.red deer (cervus elaphus l.) are very valuable in trophy-hunting but also contribute to the preservation of natural areas. they are affected by many parasites and pathogens, including hard ticks that are not only important parasites themselves but can also act as vectors and/or reservoirs of pathogens. tick phenology is complex insofar as population dynamics depend on environmental conditions, vegetation, host availability and their own intrinsic characteristic. ticks were collected monthly from ...201626715543
morphology, epidemiology, and phylogeny of babesia: an overview.babesiosis is a tick-borne hemoprotozoan disease of domestic and wild animals. the disease is caused by various species of babesia and some species of babesia have also zoonotic significance. the parasite in vertebrate hosts' remains in erythrocytes and the morphology of babesia spp. is not uniform in all vertebrate hosts. with the advancement of science, particularly the use of molecular techniques made it easy to study the evolution of parasites and thereby reclassifying babesia spp. as per th ...026629451
vectors of crimean congo hemorrhagic fever virus in iran.ticks are important vectors and reservoirs of crimean congo hemorrhagic fever (cchf) virus. human beings may be infected whenever the normal life cycle of the infected ticks on non-human vertebrate hosts is interrupted by the undesirable presence of humans in the cycle. a total of 26 species of argasid and ixodid ticks have been recorded in iran; including nine hyalomma, two rhipicephalus, two dermacentor, five haemaphysalis, two boophilus, one ixodes and two argas as well as three ornithodoros ...201526623426
geographical distribution of dermacentor marginatus and dermacentor reticulatus in europe.the goal of this paper is to present up-to-date maps depicting the geographical distribution of dermacentor species in europe based on georeferenced sampling sites. therefore, a dataset was compiled, resulting in 1286 d. marginatus (sulzer, 1776) and 1209 d. reticulatus (fabricius, 1794) locations. special emphasis is given to the region of the european alps depicting a presumable climate barrier of the mountains and to overlaps in the distribution of both species as well as on the situation in ...201626552893
divergent viruses discovered in arthropods and vertebrates revise the evolutionary history of the flaviviridae and related viruses.viruses of the family flaviviridae are important pathogens of humans and other animals and are currently classified into four genera. to better understand their diversity, evolutionary history, and genomic flexibility, we used transcriptome sequencing (rna-seq) to search for the viruses related to the flaviviridae in a range of potential invertebrate and vertebrate hosts. accordingly, we recovered the full genomes of five segmented jingmenviruses and 12 distant relatives of the known flavivirida ...201526491167
a preliminary investigation on ticks (acari: ixodidae) infesting birds in kızılırmak delta, turkey.ticks are mandatory blood-feeding ectoparasites of mammals, birds, reptiles, and even amphibians. turkey has a rich bird fauna and is located on the main migration route for many birds. however, information on ticks infesting birds is very limited. in the present study, we aimed to determine ticks infesting birds in kızılırmak delta, turkey. in 2014 autumn bird migration season, a total of 7,452 birds belonging to 79 species, 52 genera, 35 families, and 14 orders were examined for tick infestati ...201626487249
long-term ecological study of host-seeking adults of hyalomma lusitanicum (acari: ixodidae) in a meso-mediterranean climate.from january 2007 to december 2014, three representative meso-mediterranean bioclimatic environment types were sampled monthly using blanket-dragging techniques to determine the tick abundance rate. hyalomma lusitanicum koch, 1844 was the most prevalent species (96.58%) followed by dermacentor marginatus sulzer, 1776; rhipicephalus pusillus gil collado, 1936; and rhipicephalus bursa canestrini and fanzago, 1878. h. lusitanicum adults begin questing activity around march, numbers rising quickly r ...201626477051
midgut proteome of an argasid tick, ornithodoros erraticus: a comparison between unfed and engorged females.the argasid tick ornithodoros erraticus is the vector of african swine fever virus and of several borrelia species that cause human relapsing fever in the iberian peninsula. the tick midgut is part of the ectoparasite-host interface and expresses proteins that are vital for the survival of the tick. midgut proteins are therefore potential targets for drug and/or vaccine design aimed at the development of new strategies for tick control. thus, the aim of this work was the characterization of the ...201526459090
emerging tick-borne infections in mainland china: an increasing public health threat.since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ...201526453241
molecular detection of anaplasma phagocytophilum and borrelia burgdorferi sensu lato genospecies in red foxes (vulpes vulpes) from romania.red foxes (vulpes vulpes) are one of the most widespread wild carnivores in the world, being recognized to harbor and transmit a wide range of vector-borne diseases. anaplasma phagocytophilum and borrelia burgdorferi sensu lato are zoonotic tick-borne pathogens causing emerging diseases. wild animals play an essential role in the transmission of diseases and pathogens maintenance in nature. epidemiological studies regarding the prevalence of tick-borne pathogens in red foxes are of public health ...201526449360
molecular survey of tick-borne pathogens in ixodid ticks collected from hunted wild animals in tuscany, italy.to determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals.201526433656
isolation and maintenance of rickettsia raoultii in a rhipicephalus sanguineus tick cell line.rickettsia raoultii, a member of the spotted fever group rickettsiae, has been implicated in cases of debonel/tibola/senlat, and has been detected in dermacentor spp. and rhipicephalus pumilio ticks by pcr. r. raoultii has been isolated in mammalian and tick cell lines. this study aimed to isolate r. raoultii from spanish dermacentor marginatus in tick cell lines. a single adult d. marginatus collected from vegetation in la rioja (northen spain) in october 2012 was surface-sterilised, triturated ...201626428859
abundance of questing ticks and molecular evidence for pathogens in ticks in three parks of emilia-romagna region of northern italy.infectious and parasitic diseases transmitted by ticks, such as lyme diseases, granulocytic anaplasmosis and piroplasmosis, have been frequently reported in europe, with increasing attention to them as an emerging zoonotic problem. the presented study was performed to assess the distribution and the density of questing ticks in three regional parks of emilia-romagna region of northern italy, and to seek molecular evidence of potential human pathogens in tick populations.201526403115
environmental monitoring and surveillance of rodents and vectors for francisella tularensis following outbreaks of human tularemia in georgia.tularemia is a re-emerging bacterial zoonosis, broadly distributed across the northern hemisphere. in georgia, there is a history of human tularemia outbreaks dating back to the 1940s. in response to outbreaks, health officials initiated long-term field surveillance and environmental monitoring. the objective of our study was to obtain information from 57 years of field surveys to identify species that play a role in the occurrence francisella tularensis subsp. holarctica in the environment in g ...201526394283
ticks (acari: ixodida) parasitizing humans in corum and yozgat provinces, turkey.in order to identify ticks infesting humans in corum and yozgat provinces in turkey, a total of 2110 ticks representing 14 species were collected on humans, between june and september 2009. of those, 1551 (687♂, 450♀, 407 nymphs, 7 larvae) were collected from corum and 559 (330♂, 180♀, 49 nymphs) were collected from yozgat. the majority of ticks (n = 1121, 53.1 %) was hyalomma marginatum. other common ticks infesting humans were dermacentor marginatus (n = 209, 9.9 %) and rhipicephalus turanicus ...201526385209
host-feeding behaviour of dermacentor reticulatus and dermacentor marginatus in mono-specific and inter-specific infestations.given the sympatric occurrence in some regions of europe and the great epidemiological significance of d. reticulatus and d. marginatus species, we investigated the behaviour of these ticks during inter-specific and mono-specific host infestations.201526381387
a broad-range survey of ticks from livestock in northern xinjiang: changes in tick distribution and the isolation of borrelia burgdorferi sensu stricto.borreliosis is highly prevalent in xinjiang uygur autonomous region, china. however, little is known about the presence of borrelia pathogens in tick species in this region, in addition borrelia pathogens have not been isolated from domestic animals.201526337627
climate suitability for european ticks: assessing species distribution models against null models and projection under ar5 climate.there is increasing evidence that the geographic distribution of tick species is changing. whilst correlative species distribution models (sdms) have been used to predict areas that are potentially suitable for ticks, models have often been assessed without due consideration for spatial patterns in the data that may inflate the influence of predictor variables on species distributions. this study used null models to rigorously evaluate the role of climate and the potential for climate change to ...201526310856
detection of lyme disease and q fever agents in wild rodents in central italy.the maintenance of tick-borne disease agents in the environment strictly depends on the relationship between tick vectors and their hosts, which act as reservoirs for these pathogens. a pilot study aimed to investigate wild rodents as reservoirs for zoonotic tick-borne pathogens (borrelia burgdorferi sensu lato (s.l.), coxiella burnetii, francisella tularensis, and anaplasma phagocytophilum) was carried out in an area of gran sasso e monti della laga national park (abruzzi region, central italy) ...201526134933
laboratory development of dermacentor marginatus ticks (acari: ixodidae) at two temperatures.the influence of two temperatures on the development of dermacentor marginatus evolutive cycle was studied. tests performed under controlled laboratory conditions at 21 °c, 80 % rh and 27 °c, rh 80 %, on ten fully engorged female ticks collected from naturally infested goats, in cluj county, romania. hatched larvae were fed on white mice and the nymphs and adults on guinea pigs. the following parameters were evaluated: egg incubation; pre-feeding, feeding and pre-moulting for larvae and nymphs; ...201526122968
species diversity and distribution of ticks (acari: ixodidae) in zabol county, eastern iran.ticks are important vectors of human and animal pathogens. they are considered as main vectors for transmission of many viral, bacterial, rickettsial and parasitical pathogens. the aim of the present study was to find out species diversity of ticks, which infested the domestic ruminants in zabol county, eastern iran in 2012.201426114136
[distribution of tick species spread in kütahya vicinity].this study was performed to determine the tick species infested to the cattle and small ruminants in kütahya province and their seasonal activity and prevalence.201526081885
identification of tick species and disseminate pathogen using hemolymph by maldi-tof ms.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. the challenges of the present study were to assess maldi-tof ms profiling for the both identification of tick species and rickettsia spp. in infected ticks using hemolymph as protein mixture.201526051210
eco-epidemiology of borrelia miyamotoi and lyme borreliosis spirochetes in a popular hunting and recreational forest area in hungary.borrelia miyamotoi, the newly discovered human pathogenic relapsing fever spirochete, and borrelia burgdorferi sensu lato are maintained in natural rodent populations. the aim of this study was to investigate the natural cycle of b. miyamotoi and b. burgdorferi s.l. in a forest habitat with intensive hunting, forestry work and recreational activity in southern hungary.201526048373
isolation and propagation of a spiroplasma sp. from slovakian ixodes ricinus ticks in ixodes spp. cell lines.ixodes spp. ticks are known to occasionally harbour spiroplasmas - helical mycoplasmas in the class mollicutes; a previous study in slovakia reported an overall prevalence of spiroplasma ixodetis of 3% in ixodes ricinus. in the present study, extracts of unfed adult i. ricinus ticks collected from vegetation in south-western slovakia were added to a panel of cell lines derived from i. ricinus and ixodes scapularis embryos. the cultures were monitored by preparation and examination of giemsa-stai ...201526003954
the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii.q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ...201525978383
theileria annae (syn. babesia microti-like) infection in dogs in nw spain detected using direct and indirect diagnostic techniques: clinical report of 75 cases.in north-western spain, piroplamosis caused by theileria annae is now recognized as a serious problem because veterinarians, despite being aware of the clinical signs of piroplasmosis, lack the necessary information on its epidemiology or specific diagnostic tools for its management. this, along with the fact that t. annae infection is also refractory to current piroplamosis treatments, prompted this study designed to assess the clinical presentation and diagnosis of this largely unknown parasit ...201525890106
prevalence of 'candidatus rickettsia vini' in ixodes arboricola ticks in the north of spain, 2011-2013.the prevalence of rickettsia spp. in ixodes arboricola ticks collected from birds in two locations in the north of spain from 2011 to 2013 was studied.201525889739
preliminary investigation on tick fauna in the neighborhood of tarquinia, lazio, italy.this study represents a preliminary inquiry on tick fauna composition carried out in maremma, central italy, where very few data are available.201525857387
a survey of ticks (acari: ixodida) infesting some wild animals from sivas, turkey.in order to determine the species composition of infesting ticks, between 2011 and 2012 a total of 1118 wild animals were captured from various regions of zara, sivas province, turkey. a total of 138 ticks were obtained from the 58 host animals. ticks were identified as dermacentor marginatus (sulzer), haemaphysalis erinacei taurica pospelova-shtrom, haemaphysalis parva (neumann), haemaphysalis punctata canestrini and fanzago, haemaphysalis sulcata canestrini and fanzago, hyalomma marginatum koc ...201525784071
new insights into flavivirus evolution, taxonomy and biogeographic history, extended by analysis of canonical and alternative coding sequences.to generate the most diverse phylogenetic dataset for the flaviviruses to date, we determined the genomic sequences and phylogenetic relationships of 14 flaviviruses, of which 10 are primarily associated with culex spp. mosquitoes. we analyze these data, in conjunction with a comprehensive collection of flavivirus genomes, to characterize flavivirus evolutionary and biogeographic history in unprecedented detail and breadth. based on the presumed introduction of yellow fever virus into the americ ...201525719412
detection of dermacentor marginatus and a possible rickettsia slovaca case in the united kingdom - the risk of the visiting traveller. 201625702622
putative novel lineage of west nile virus in uranotaenia unguiculata mosquito, hungary.west nile virus (wnv) is an increasing public health concern in europe with numerous human cases. a total of 23,029 female mosquitoes were tested for a variety of mosquito-borne flaviviruses and orthobunyaviruses supposedly endemic in southern transdanubia, hungary, in the frames of a large-scale surveillance between 2011 and 2013. wnv nucleic acid was detected in a single pool containing uranotaenia unguiculata mosquitoes. sequence- and phylogenetic analyses for two different regions (ns5 and e ...201425674630
detection of rickettsia spp in ticks by maldi-tof ms.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been shown to be an effective tool for the rapid identification of arthropods, including tick vectors of human diseases.201525659152
unprecedented genomic diversity of rna viruses in arthropods reveals the ancestry of negative-sense rna viruses.although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. through rna sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense rna viruses, a number of which are also present as endogenous genomic copies. with this greatly enriched diversity we revealed that arthropods contain ...201525633976
novel candidatus rickettsia species detected in nostril tick from human, gabon, 2014.we report the identification of a nymphal nostril tick (amblyomma sp.) from a national park visitor in gabon and subsequent molecular detection and characterization of tickborne bacteria. our findings provide evidence of a potentially new rickettsia sp. circulating in africa and indicate that tick bites may pose a risk to persons visiting parks in the region.201525625886
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
occurrence of ixodiphagus hookeri (hymenoptera: encyrtidae) in ixodes ricinus (acari: ixodidae) in southern italy.natural enemies of ticks include the parasitoid wasp ixodiphagus hookeri (hymenoptera: encyrtidae). the aim of this preliminary study was to investigate the occurrence of i. hookeri dna in a community of ticks (ixodes ricinus, dermacentor marginatus, hyalomma marginatum, haemaphysalis inermis and rhipicephalus turanicus). from may 2010 to march 2012, ticks were collected monthly by dragging and flagging, identified, and 481 adults and 305 nymphs screened molecularly for infection with i. hookeri ...201525614120
molecular characterization of a defensin gene from a hard tick, dermacentor silvarum.ticks are distributed worldwide and considered as vectors of many human diseases. tick defensins, a family of antimicrobial peptides, form the first line of defense against pathogens.201525588982
whole genome phylogenetic investigation of a west nile virus strain isolated from a tick sampled from livestock in north eastern kenya.west nile virus (wnv) has a wide geographical distribution and has been associated to cause neurological disease in humans and horses. mosquitoes are the traditional vectors for wnv; however, the virus has also been isolated from tick species in north africa and europe which could be a means of introduction and spread of the virus over long distances through migratory birds. although wnv has been isolated in mosquitoes in kenya, paucity of genetic and pathogenicity data exists. we previously rep ...201425430727
putative new west nile virus lineage in uranotaenia unguiculata mosquitoes, austria, 2013.west nile virus (wnv) is becoming more widespread and markedly effecting public health. we sequenced the complete polyprotein gene of a divergent wnv strain newly detected in a pool of uranotaenia unguiculata mosquitoes in austria. phylogenetic analyses suggest that the new strain constitutes a ninth wnv lineage or a sublineage of wnv lineage 4.025418009
bunyavirus-vector interactions.the bunyaviridae family is comprised of more than 350 viruses, of which many within the hantavirus, orthobunyavirus, nairovirus, tospovirus, and phlebovirus genera are significant human or agricultural pathogens. the viruses within the orthobunyavirus, nairovirus, and phlebovirus genera are transmitted by hematophagous arthropods, such as mosquitoes, midges, flies, and ticks, and their associated arthropods not only serve as vectors but also as virus reservoirs in many cases. this review present ...201425402172
phytotherapeutic activity of euphorbia cyparissias extracts on ixodidae (acari) female ticks.given its numerous biologically active components, euphorbiaceae has been found to be a large plant family and polyvalent with quite interesting therapeutic activity that can be studied.201425392580
the first german map of georeferenced ixodid tick locations.georeferenced locations of ixodid ticks are required to depict the observed distribution of species. further, they are used as input data for species distribution models also known as niche models. the latter were applied to describe current and future (projected) tick distributions. beside model assumptions and selected climate parameters, the number of georeferenced tick locations available as a digital dataset is of fundamental importance for the reliability of such models. for germany, howev ...201425301245
'candidatus rickettsia asemboensis' and wolbachia spp. in ctenocephalides felis and pulex irritans fleas removed from dogs in ecuador.flea-borne infections are distributed worldwide. up to date there are no reports about microorganisms associated to fleas in ecuador.201425266919
crimean-congo hemorrhagic fever virus clades v and vi (europe 1 and 2) in ticks in kosovo, 2012.despite being a small country, kosovo represents one of the few foci of crimean-congo hemorrhagic fever (cchf) in europe. the distribution of kosovar tick vectors and the evolution of cchf virus in ticks are both as yet unknown. a better description of the extent and the genetic diversity of cchfv in ticks from endemic settings is essential, in order to be controlled. we investigated the 2012 distribution of kosovar ticks alongside the prevalence and the phylogeography of tick-derived cchfv. hya ...201425255381
prevalence and phylogenetic analysis of tick-borne encephalitis virus (tbev) in field-collected ticks (ixodes ricinus) in southern switzerland.tick-borne encephalitis is the most common tick-borne viral infection in europe with 3,000 human cases reported each year. in western europe, the castor bean tick, ixodes ricinus, is the principal vector of the tick-borne encephalitis virus (tbev). tbev appears to be spreading geographically and was recently detected for the first time in canton valais in the southern part of switzerland. the purpose of the present study was to survey the i. ricinus tick populations of canton valais for tbev.201425245773
survey on cattle ticks in nur, north of iran.to survey the prevalence of cattle ticks in nur county and prepare a list of tick fauna in this district.201425182439
isolation and characterization of babesia pecorum sp. nov. from farmed red deer (cervus elaphus).the diversity of babesia species infecting cervids in parts of central and southern spain was analyzed by collecting blood from farmed red deer (cervus elaphus). babesia sp. was isolated in vitro from two red deer herds in cádiz and ciudad real. the number of babesia sp. carriers differed between the two herds: 36/77 in cádiz and 1/35 in ciudad real. hyalomma lusitanicum was the most prevalent tick species identified on the cádiz farm vegetation and on sampled animals, and is therefore a candida ...201425155988
range expansion of ixodes ricinus to higher altitude, and co-infestation of small rodents with dermacentor marginatus in the northern apennines, italy.immature ticks (ixodes ricinus and dermacentor marginatus) were collected from small rodents (apodemus spp. and myodes glareolus), in the northern apennines, italy, at an altitude up to 1650 m above sea level (a.s.l.), from 2009 through 2012. while d. marginatus had been found at the same location in studies carried out in 1994, i. ricinus was very rare or absent. prevalence (95% confidence interval) of infestation by i. ricinus larvae on apodemus spp. was 54.4% (47.5, 61.2), and it was greater ...201425139531
high-throughput screening of tick-borne pathogens in europe.due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout europe. therefore, extended surveillance tools are desirable. to accurately screen tick-borne pathogens (tbps), a large scale epidemiological study was conducted on 7050 ixodes ricinus nymphs collected from france, denmark, and the netherlands using a powerful new high-throughput approach. this advanced methodology permitted the simultaneous detect ...201425120960
identification of tick-borne pathogens in ticks feeding on humans in turkey.the importance of tick-borne diseases is increasing all over the world, including turkey. the tick-borne disease outbreaks reported in recent years and the abundance of tick species and the existence of suitable habitats increase the importance of studies related to the epidemiology of ticks and tick-borne pathogens in turkey. the aim of this study was to investigate the presence of and to determine the infection rates of some tick-borne pathogens, including babesia spp., borrelia burgdorferi se ...201425101999
tick-borne lymphadenopathy, an emerging disease.tick-borne lymphadenopathy (tibola) is a spotted fever group disease characterized by an eschar and pronounced enlarged lymph nodes after a scalp tick bite. the goal of this synopsis is to review the tibola literature published until may 2013: forty-one articles (reporting 537 cases) were included. there was a predominance of cases in females and young people. spain, france, and hungary reported the majority of cases, and they were mainly reported in the colder seasons. the involved tick bite wa ...201425090977
the role of cats in the eco-epidemiology of spotted fever group diseases.mediterranean spotted fever (msf), whose etiological agent is r. conorii, is one of the oldest described vector-borne infectious diseases. although it is endemic in the mediterranean area, clinical cases have also been reported in other regions. r. massiliae-bar29 is related to msf cases. this strain is distributed worldwide. r. conorii and r. massiliae-bar29 are transmitted by ticks. dogs are considered the sentinel of r. conorii infection. cats could also be involved in their transmission. ric ...201425084969
abstracts of the 1st conference on neglected vectors and vector-borne diseases (eurnegvec): with management committee and working group meetings of the cost action td1303, april 8-11, 2014, cluj-napoca, romania. 201425078468
phylogenetic analysis of west nile virus genome, iran. 025061976
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