Publications

antimicrobial peptide gramicidin s is accumulated in granules of producer cells for storage of bacterial phosphagens.	many antimicrobial peptides are synthesized non-ribosomally in bacteria, but little is known about their subcellular route of biosynthesis, their mode of intracellular accumulation, or their role in the physiology of the producer cells. here, we present a comprehensive view on the biosynthesis of gramicidin s (gs) in aneurinibacillus migulanus, having observed a peripheral membrane localization of its synthetases. the peptide gets accumulated in nano-globules, which mature by fusion into larger ...	2017	28295017
affinity purification method for the identification of nonribosomal peptide biosynthetic enzymes using a synthetic probe for adenylation domains.	a series of inhibitors have been designed based on 5'-o-sulfamoyl adenosine (ams) that display tight binding characteristics towards the inhibition of adenylation (a) domains in nonribosomal peptide synthetases (nrpss). we recently developed an affinity probe for a domains that could be used to facilitate the specific isolation and identification of nrps modules. our synthetic probe, which is a biotinylated variant of l-phe-ams (l-phe-ams-biotin), selectively targets the a domains in nrps module ...	2016	26831701
strain-level diversity of secondary metabolism in the biocontrol species aneurinibacillus migulanus.	aneurinibacillus migulanus strains nagano and nctc 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of ascomycota, basidiomycota, and oomycetes. differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a single antibiotic cyclic peptide, gramicidin s (gs), by the two strains, is not the sole mechanism of inhibition. the availability of four sequenced genomes of aneurinibacillus prompted ...	2016	26686620
complete genome sequence of aneurinibacillus migulanus e1, a gramicidin s- and d-phenylalanyl-l-propyl diketopiperazine-deficient mutant.	we report here the complete genome sequence of the aneurinibacillus migulanus e1 mutant deficient in gramicidin s (gs) and d-phenylalanyl-l-propyl diketopiperazine (dkp) formation. the genome consists of a circular chromosome (6,301,904 bp, 43.20% g+c content) without any plasmid. the complete genome sequence enables further investigation of the biosynthetic mechanism and the biological function of gramicidin s.	2015	26679577
aneurinibacillus humi sp. nov., isolated from soil collected in ukraine.	a novel bacterium, designated u33(t), was isolated from a soil sample collected in mykhailyky, poltavs'ka oblast, ukraine. the bacterium was aerobic, gram-positive, spore-forming, and consists of motile rods. the taxonomic position of strain u33(t) was studied by a polyphasic approach, and the results clearly showed that the phenotypic and chemotaxonomic properties are consistent with those of the genus aneurinibacillus. the phylogenic analysis with 16s rrna gene sequence of strains u33(t) showe ...	2016	26542530
high-quality draft genome sequence of aneurinibacillus migulanus atcc 9999t (dsm 2895), a gramicidin s-producing bacterium isolated from garden soil.	aneurinibacillus migulanus atcc 9999(t) (dsm 2895) is a gram-positive, round-spore-forming, and gramicidin s-producing bacterium. here, we report the 6.35-mb high-quality draft genome sequence of a. migulanus atcc 9999(t), which will provide useful information for the genomic taxonomy and phylogenomics of bacillus-like bacteria.	2015	26494674
a multiple-labeling strategy for nonribosomal peptide synthetases using active-site-directed proteomic probes for adenylation domains.	genetic approaches have greatly contributed to our understanding of nonribosomal peptide biosynthetic machinery; however, proteomic investigations are limited. here, we developed a highly sensitive detection strategy for multidomain nonribosomal peptide synthetases (nrpss) by using a multiple-labeling technique with active-site-directed probes for adenylation domains. when applied to gramicidin s-producing and -nonproducing strains of aneurinibacillus migulanus (dsm 5759 and dsm 2895, respective ...	2015	26467472
draft genome sequence of aneurinibacillus migulanus nctc 7096.	aneurinibacillus migulanus has biocontrol activities against fungal, fungus-like, and bacterial plant pathogens with different levels of efficacy depending on the target pathogens. here, we report the high-quality draft genome sequence of a. migulanus nctc 7096.	2015	25838489
draft genome sequence of aneurinibacillus migulanus strain nagano.	aneurinibacillus migulanus is characterized by inhibition of growth of a range of plant-pathogenic bacteria and fungi. here, we report the high-quality draft genome sequences of a. migulanus nagano.	2015	25838487
aneurinibacillus tyrosinisolvens sp. nov., a tyrosine-dissolving bacterium isolated from organics- and methane-rich seafloor sediment.	a novel gram-positive-staining, strictly aerobic and heterotrophic bacterium, designated strain ll-002t, was isolated from organics- and methane-rich seafloor sediment at a depth of 100 m in kagoshima bay, kagoshima, japan. colonies were lustreless and translucent white in colour. the temperature, ph and salt concentration ranges for growth were 10-30 °c, ph 6.0-6.5 and 0-1 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences confirmed that strain ll-002t belongs to the genus ane ...	2015	25813364
fermentation and cost-effective 13c/15n labeling of the nonribosomal peptide gramicidin s for nuclear magnetic resonance structure analysis.	gramicidin s (gs) is a nonribosomally synthesized decapeptide from aneurinibacillus migulanus. its pronounced antibiotic activity is attributed to amphiphilic structure and enables gs interaction with bacterial membranes. despite its medical use for over 70 years, the peptide-lipid interactions of gs and its molecular mechanism of action are still not fully understood. therefore, a comprehensive structural analysis of isotope-labeled gs needs to be performed in its biologically relevant membrane ...	2015	25795666
direct surfactin-gramicidin s antagonism supports detoxification in mixed producer cultures of bacillus subtilis and aneurinibacillus migulanus.	antibiotic production as a defence mechanism is a characteristic of a wide variety of organisms. in natural evolutionary adaptation, cellular events such as sporulation, biofilm formation and resistance to antibiotics enable some micro-organisms to survive environmental and antibiotic stress conditions. the two antimicrobial cyclic peptides in this study, gramicidin s (gs) from aneurinibacillus migulanus and the lipopeptide surfactin (srf) from bacillus subtilis, have been shown to affect both m ...	2012	23103974
control of downy mildew (pseudoperonospora cubensis) of greenhouse grown cucumbers with alternative biological agents.	in organic cucumber production infection with downy mildew (pseudoperonospora cubensis) is a major problem. plant extracts from glycyrrhiza glabra l. (licorice), a plant belonging to the family fabaceae, and salvia officinalis (sage) as well as cultures of the bacterium aneurinibacillus migulanus were investigated for efficacy of disease control under commercial growing conditions. contrary to bioassays, where sage extract and the microorganism showed highest activity, in the trials of 2008 g. g ...	2010	21534461
the ability of aneurinibacillus migulanus (bacillus brevis) to produce the antibiotic gramicidin s is correlated with phenotype variation.	phenotype instability of bacterial strains can cause significant problems in biotechnological applications, since industrially useful properties may be lost. here we report such degenerative dissociation for aneurinibacillus migulanus (formerly known as bacillus brevis) an established producer of the antimicrobial peptide gramicidin s (gs). phenotypic variations within and between various strains maintained in different culture collections are demonstrated. the type strain, atcc 9999, consists o ...	2007	17720841
reclassification of brevibacillus brevis strains ncimb 13288 and dsm 6472 (=nrrl nrs-887) as aneurinibacillus danicus sp. nov. and brevibacillus limnophilus sp. nov.	comparison of the hypervariable region (269-279 bases in length) at the 5' end of the 16s rdna sequences of 29 bacterial strains that were identified previously as brevibacillus brevis showed that 13 strains clustered with aneurinibacillus species, eight strains clustered with bacillus species and eight strains clustered with brevibacillus species. based on dna-dna hybridization results, 27 strains, not including [brevibacillus brevis] ncimb 13288 and [brevibacillus brevis] dsm 6472, were reiden ...	2004	15023954
proposal for two new genera, brevibacillus gen. nov. and aneurinibacillus gen. nov.	16s rrna gene sequences of the type strains of 11 species belonging to the bacillus brevis and bacillus aneurinolyticus groups were determined. on the basis of the results of gene sequence analyses, these species were separated into two clusters. the b. brevis cluster included 10 species, namely, bacillus brevis, bacillus agri, bacillus centrosporus, bacillus choshinensis, bacillus parabrevis, bacillus reuszeri, bacillus formosus, bacillus borstelensis, bacillus laterosporus, and bacillus thermo ...	1996	8863420
characterization of bacillus brevis with descriptions of bacillus migulanus sp. nov., bacillus choshinensis sp. nov., bacillus parabrevis sp. nov., and bacillus galactophilus sp. nov.	thirty-five bacillus brevis strains obtained from culture collections, including protein-producing isolates, were taxonomically studied by using numerical analysis, dna base composition, and dna-dna hybridization. six dna relatedness groups were represented, and these groups correlated well with clusters based on the numerical analysis. the b. brevis strains were separated into b. brevis sensu stricto, four new species, and an unidentified species of the genus bacillus. bacillus migulanus sp. no ...	1993	8494737