Publications

gene flow inferred from seed dispersal and pollinator behaviour compared to dna analysis of restriction site variation in a patchy population of lotus corniculatus l.	gene flow was investigated in a natural population of lotus corniculatus l. (fabaceae) using a combination of pollen and seed dispersal studies and a recombinant dna technique. the population is spatially heterogeneous and grows with empetrum nigrum. l. corniculatus is pollinated by the pollen-collecting bumblebee bombus lapidarius l. most pollinator flights occurred within patches, as bees usually visit nearest-neighbour plants, show no marked directionality, and forage mostly within patches. g ...	1992	28312883
why do honeybees reject certain flowers?	honeybees often approach flowers of lotus corniculatus and then fly away without attempting to extract nectar. these rejected flowers contained 41% less nectar than my random sample. the accepted flowers contained 24% more nectar than my random sample. the differences among these three flower-groups were due to differences in the percent of empty flowers in each group rather than the differences in the absolute amount of nectar. honeybees increased their foraging efficiency by accepting less emp ...	1986	28311617
effects of elevated co2 on flowering phenology and nectar production of nectar plants important for butterflies of calcareous grasslands.	effects of elevated co2 on flowering phenology and nectar production were investigated in trifolium pratense, lotus corniculatus, scabiosa columbaria, centaurea jacea and betonica officinalis, which are all important nectar plants for butterflies. in glasshouse experiments, juvenile plants were exposed to ambient (350 μl l(-1)) and elevated (660 μl l(-1)) co2 concentrations for 60-80 days. elevated co2 significantly enhanced the development of flower buds in c. jacea. b. officinalis flowered ear ...	1998	28307818
replacing alfalfa silage with tannin-containing birdsfoot trefoil silage in total mixed rations for lactating dairy cows.	two lactation trials were conducted comparing the feeding value of silages made from birdsfoot trefoil (bft, lotus corniculatus l.) that had been selected for low (bftl), medium (bftm), and high (bfth) levels of condensed tannins (ct) to an alfalfa silage (as) when fed as the principal forage in total mixed rations. diets also included corn silage, high-moisture shelled corn, soybean meal, soy hulls, and supplemental fat. in trial 1, 32 lactating holstein cows were blocked by days in milk, assig ...	2017	28259401
mesorhizobium olivaresii sp. nov. isolated from lotus corniculatus nodules.	in this study four mesorhizobium strains isolated from lotus corniculatus nodules in granada (spain) were characterized. their 16s rrna gene sequences were closely related to those of m. albiziae lmg 23507(t) and m. chacoense pr5(t) showing 99.4 and 99.2% similarity values, respectively. the analysis of concatenated rpob, reca, atpd and glnii genes showed they formed a cluster with internal similarities higher than 97%. the closest species also were m. albiziae lmg 23507(t) and m. chacoense pr5( ...	2016	27712916
chronic exposure to a neonicotinoid pesticide alters the interactions between bumblebees and wild plants.	insect pollinators are essential for both the production of a large proportion of world crops and the health of natural ecosystems. as important pollinators, bumblebees must learn to forage on flowers to feed both themselves and provision their colonies.increased use of pesticides has caused concern over sublethal effects on bees, such as impacts on reproduction or learning ability. however, little is known about how sublethal exposure to field-realistic levels of pesticide might affect the abil ...	2016	27512241
effect of exchanging onobrychis viciifolia and lotus corniculatus for medicago sativa on ruminal fermentation and nitrogen turnover in dairy cows.	the objective of the study was to determine the effect of feeding sainfoin (sf; onobrychis viciifolia) and birdsfoot trefoil (bt; lotus corniculatus), 2 temperate climate forage legumes that contain condensed tannins (ct), on ruminal fermentation and n turnover in dairy cows. six ruminally cannulated multiparous dairy cows (milk yield=40kg/d; 36 d in milk) were used in a replicated 3×3 latin square design. all animals were fed basal diets containing 20% pelleted sf (223g of ct/kg of dry matter), ...	2016	26995129
plants increase arsenic in solution but decrease the non-specifically bound fraction in the rhizosphere of an alkaline, naturally rich soil.	we aimed at determining the major physical-chemical processes that drive arsenic (as) dynamic in the rhizosphere of four species (holcus lanatus, dittrichia viscosa, lotus corniculatus, plantago lanceolata) tested for phytostabilization. experiments were performed with an alkaline soil naturally rich in as. composition of the soil solution of planted and unplanted pots was monitored every 15 days for 90 days, with a focus on the evolution of as concentrations in solution and in the non-specifica ...	2016	26707185
therapeutic potential of temperate forage legumes: a review.	the discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. temperate forage legumes are fabaceae species that include worldwide-important crops. these plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. we have examined here medicago sativa (alfalfa), trif ...	2016	26507574
soil-to-plant transfer of native selenium for wild vegetation cover at selected locations of the czech republic.	total selenium (se) contents were determined in aboveground biomass of wild plant species growing in two uncultivated meadows at two different locations. the soils in these locations had pseudototal (aqua regia soluble) se in concentration ranges of between 0.2 and 0.3 mg kg(-1) at the first location, and between 0.7 and 1.4 mg kg(-1) at the second location. the plant species represented 29 plant families where the most numerous ones were poaceae, rosaceae, fabaceae , and asteraceae. the seleniu ...	2015	25975239
carcass parameters and meat quality in meat-goat kids finished on chicory, birdsfoot trefoil, or red clover pastures.	this study was conducted during the 2009 and 2010 grazing seasons to assess carcass parameters and chevon (goat meat) quality when meat-goat kids (n=72) were finished on pastures of red clover (trifolium pratense l.; rcl), birdsfoot trefoil (lotus corniculatus l.; bft), or chicory (cichorium intybus l.; chic). final live weight (p<0.05) and carcass weight (p=0.10) were greater when goats were finished on rcl compared to chic with bft being intermediate. ribeye area, backfat thickness, body wall ...	2015	25817803
genome sequence of the lotus spp. microsymbiont mesorhizobium loti strain r7a.	mesorhizobium loti strain r7a was isolated in 1993 in lammermoor, otago, new zealand from a lotus corniculatus root nodule and is a reisolate of the inoculant strain icmp3153 (nzp2238) used at the site. r7a is an aerobic, gram-negative, non-spore-forming rod. the symbiotic genes in the strain are carried on a 502-kb integrative and conjugative element known as the symbiosis island or icemlsym(r7a). m. loti is the microsymbiont of the model legume lotus japonicus and strain r7a has been used exte ...	2014	25780499
genome sequence of the lotus corniculatus microsymbiont mesorhizobium loti strain r88b.	mesorhizobium loti strain r88b was isolated in 1993 in the rocklands range in otago, new zealand from a lotus corniculatus root nodule. r88b is an aerobic, gram-negative, non-spore-forming rod. this report reveals the genome of m. loti strain r88b contains a single scaffold of size 7,195,110 bp which encodes 6,950 protein-coding genes and 66 rna-only encoding genes. this genome does not harbor any plasmids but contains the integrative and conjugative element icemlsym(r7a), also known as the r7a ...	2014	25780496
growth and survival of mesorhizobium loti inside acanthamoeba enhanced its ability to develop more nodules on lotus corniculatus.	the importance of protozoa as environmental reservoirs of pathogens is well recognized, while their impact on survival and symbiotic properties of rhizobia has not been explored. the possible survival of free-living rhizobia inside amoebae could influence bacterial abundance in the rhizosphere of legume plants and the nodulation competitiveness of microsymbionts. two well-characterized strains of mesorhizobium: mesorhizobium loti nzp2213 and mesorhizobium huakuii symbiovar loti maff303099 were a ...	2015	25779926
revision of the taxonomic status of type strains of mesorhizobium loti and reclassification of strain usda 3471t as the type strain of mesorhizobiumerdmanii sp. nov. and atcc 33669t as the type strain of mesorhizobiumjarvisii sp. nov.	the species mesorhizobim loti was isolated from nodules of lotus corniculatus and its type strain deposited in several collections. some of these type strains, such as those deposited in the usda and atcc collections before 1990, are not coincident with the original strain, nzp 2213t, deposited in the nzp culture collection. the analysis of the 16s rrna gene showed that strains usda 3471t and atcc 33669t formed independent branches from that occupied by mesorhizobium loti nzp 2213t and related t ...	2015	25736411
interrelations between herbage yield, α-tocopherol, β-carotene, lutein, protein, and fiber in non-leguminous forbs, forage legumes, and a grass-clover mixture as affected by harvest date.	pastures with diverse botanical composition may enhance animal-derived product quality. a recent study demonstrated high vitamin concentrations and yields in some forb species. the objectives of the present study were to investigate interrelations between herbage yields, vitamin concentrations, protein and fiber contents and analyze the effect of harvest date. we hypothesized that interrelations would be similar across investigated forage species. four nonleguminous forbs: salad burnet (sanguiso ...	2015	25573460
genome sequence of ensifer arboris strain lmg 14919(t); a microsymbiont of the legume prosopis chilensis growing in kosti, sudan.	ensifer arboris lmg 14919(t) is an aerobic, motile, gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of several species of legume trees. lmg 14919(t) was isolated in 1987 from a nodule recovered from the roots of the tree prosopis chilensis growing in kosti, sudan. lmg 14919(t) is highly effective at fixing nitrogen with p. chilensis (chilean mesquite) and acacia senegal (gum arabic tree or gum acacia). lmg 14919(t) does not nodulate the tree ...	2014	25197433
determination of nutrient contents and in vitro gas production values of some legume forages grown in the harran plain saline soils.	the aim of this study was to determine the nutritive value of some legume species in salt-affected soils of south-east anatolian region using chemical composition and in vitro gas production kinetics. in this study, lotus corniculatus, trifolium alexandrinum, medicago sativa were sown and tested in four different locations. a 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (ec)<4 ds/m; low salt: 4 ds/m>ec<8 ds/m, medium saline: 8 ds/m>ec<16 ds/m ...	2014	25050020
in vitro screening of forty medicinal plant extracts from the united states northern great plains for anthelmintic activity against haemonchus contortus.	an egg hatch assay (eha) and a larval migration assay (lma) involving haemonchus contortus was used to evaluate the anthelmintic activity of methanol extracts from 40 plants that are native or naturalized within the u.s.a. northern great plains. only one of these 40 plants (i.e. lotus corniculatus) had been previously evaluated for activity against any gastrointestinal nematode. the various extracts were initially screened at 50mg/ml diluted either in 0.5% dimethyl sulphoxide (dmso) or 3-(n-morp ...	2014	24548703
phyllobacterium loti sp. nov. isolated from nodules of lotus corniculatus.	strain s658(t) was isolated from a lotus corniculatus nodule in a soil sample obtained in uruguay. phylogenetic analysis of the 16s rrna gene and atpd gene showed that this strain clustered within the genus phyllobacterium. the closest related species was, in both cases, phyllobacterium trifolii petp02(t) with 99.8 % sequence similarity in the 16s rrna gene and 96.1 % in the atpd gene. the 16s rrna gene contains an insert at the beginning of the sequence that has no similarities with other inser ...	2014	24271211
fatty acids, α-tocopherol, β-carotene, and lutein contents in forage legumes, forbs, and a grass-clover mixture.	fresh forages are an important natural source of vitamins and fatty acids in ruminant diets, and their concentrations in forage species are important for the quality of animal-derived foods such as dairy and meat products. the aims of this study were to obtain novel information on vitamins and fatty acids (fa) in a variety of forage legumes and non-legume forb species compared to a grass-clover mixture and to explore implications for animal-derived products. seven dicotyledons [four forbs (salad ...	2013	24262011
transformation of forage legumes using agrobacterium tumefaciens.	galls were induced in six species of forage legumes following inoculation with wild-type strains of a. tumefaciens. the plant species was more influential than the bacterial strain in determining the type of tumour produced. inoculation of medicago sativa resulted in small, disorganised tumours. the three trifolium species, t. repens, t. hybridum and t. pratense, formed galls which tended to produce roots and both onobrychis viciifolia and lotus corniculatus produced teratomatous galls. the shoo ...	1986	24247771
phenylpropanoid defence responses in transgenic lotus corniculatus 1. glutathione elicitation of isoflavan phytoalexins in transformed root cultures.	when agrobacterium rhizogenes transformed root cultures of lotus corniculatus were treated with glutathione, isoflavan phytoalexins accumulated in both tissue and culture medium. this accumulation of phytoalexins was preceded by a transient increase in the activity of phenylalanine ammonia lyase (pal). elicitation of pal occurred throughout the growth curve of lotus 'hairy roots' and in different sectors of transformed root material.	1991	24221394
variation in a single protoplast- and seed-derived population of lotus corniculatus l.	lotus comiculatus l. is a widely cultivated, outbreeding, leguminous forage crop. seventy-one plants, most of which were tetraploid, were regenerated from calli derived from a single protoplast. their morphological and agronomic traits were evaluated and compared with those of the seed-produced population. the variances of most of the traits in the protoplast-derived (protoclonal) population were smaller than those of the seed-produced population. mean values of all the traits of the protoclonal ...	1990	24221102
genetic modification of condensed tannin biosynthesis in lotus corniculatus. 1. heterologous antisense dihydroflavonol reductase down-regulates tannin accumulation in "hairy root" cultures.	an antisense dihydroflavonol reductase (dfr) gene-construct made using the cdna for dfr from antirrhinum majus was introduced into the genome of a series of clonal genotypes of lotus corniculatus via agrobacterium rhizogenes. after initial screening, 17 antisense and 11 control transformation events were analysed and tannin levels found to be reduced in antisense root cultures. the effect of this antisense construct, (pmaj2), which consisted of the 5' half of the dfr cdna sequence, was compared ...	1994	24190536
the trehalose utilization gene thua ortholog in mesorhizobium loti does not influence competitiveness for nodulation on lotus spp.	competitiveness for nodulation is a desirable trait in rhizobia strains used as inoculant. in sinorhizobium meliloti 1021 mutation in either of the trehalose utilization genes thua or thub influences its competitiveness for root colonization and nodule occupancy depending on the interacting host. we have therefore investigated whether mutation in the thua ortholog in mesorhizobium loti maff303099 also leads to a similar competitive phenotype on its hosts. the results show that m. loti thua mutan ...	2014	24142427
does the invasive lupinus polyphyllus increase pollinator visitation to a native herb through effects on pollinator population sizes?	invasive plants may compete with native species for abiotic factors as light, space and nutrients, and have also been shown to affect native pollination interactions. studies have mainly focused on how invasive plants affect pollinator behaviour, i.e. attraction of pollinators to or away from native flowers. however, when an invasive plant provides resources utilized by native pollinators this could increase pollinator population sizes and thereby pollination success in natives. effects mediated ...	2014	24061551
two years research on efficiency of two intercrops, birdsfoot trefoil and summer savory, to reduce damage caused by onion thrips(thrips tabaci lindeman, thysanoptera, thripidae) on leek.	in 2009 and 2011, a field experiment was carried out at the laboratory field at the biotechnical faculty in ljubljana, slovenia, with the aim to investigate suitability of two intercrops, birdsfoot trefoil (lotus corniculatus l) and summer savory (satureja hortensis l.), for reducing damage caused by onion thrips (thrips tabaci lindeman) on leek (allium porrum l.). four leek cultivars--'columbus', 'forrest', 'lancelot' and 'lincoln'--were used in the research (bejo zaden b.v., netherlands). in b ...	2012	23885428
antioxidant and photosystem ii responses contribute to explain the drought-heat contrasting tolerance of two forage legumes.	identification of metabolic targets of environmental stress factors is critical to improve the stress tolerance of plants. studying the biochemical and physiological responses of plants with different capacities to deal with stress is a valid approach to reach this objective. lotus corniculatus (lotus) and trifolium pratense (clover) are legumes with contrasting summer stress tolerances. in stress conditions, which are defined as drought, heat or a combination of both, we found that differential ...	2013	23792824
in situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration.	dairy cattle often make poor use of protein when offered diets comprising high proportions of alfalfa (medicago sativa l.) hay or silage because nonprotein n formed during forage conservation and ruminal fermentation exceeds requirements for rumen microbial protein synthesis; however, condensed tannins (ct) may reduce proteolysis in the silo and in the rumen, thereby potentially improving the efficiency of crude protein (cp) use in ruminant diets. two harvests, yielding 12 hays and 12 silages ma ...	2013	23477815
conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis.	rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but the mechanisms by which they act are yet to be determined. as a first step to investigate the function of exopolysaccharide (eps) in the formation of determinate nodules, we isolated mesorhizobium loti mutants affected in various steps of eps biosynthesis and characterized their symbiotic phenotypes on two lotus spp. the wild-type m. loti r7a produced both high molecular weight eps and l ...	2013	23134480
increased plant carbon translocation linked to overyielding in grassland species mixtures.	plant species richness and productivity often show a positive relationship, but the underlying mechanisms are not fully understood, especially at the plant species level. we examined how growing plants in species mixture influences intraspecific rates of short-term carbon (c-) translocation, and determined whether such short-term responses are reflected in biomass yields. we grew monocultures and mixtures of six common c3 grassland plant species in outdoor mesocosms, applied a (13)c-co(2) pulse ...	2012	23049893
suitability of live yeast addition to alleviate the adverse effects due to the restriction of the time of access to feed in sheep fed only pasture.	the effect of yeast addition on intake and digestive utilization of pasture was studied in ovines under restricted time of access to forage. eighteen wethers housed in metabolic cages and fed fresh forage (predominantly lotus corniculatus) were randomly assigned to three treatments: forage available all day (ad); forage available only 6 h/day (r) and forage available only 6 h/day plus live saccharomyces cerevisiae yeast (ry). feed intake and digestibility, feeding behaviour, kinetics of passage, ...	2013	23020124
polymorphic infection and organogenesis patterns induced by a rhizobium leguminosarum isolate from lotus root nodules are determined by the host genotype.	to sample the natural variation in genes controlling compatibility in the legume-rhizobium symbiosis, we isolated rhizobia from nodules of endemic lotus species from 21 sites across europe. the majority of isolates were identified as mesorhizobium- or bradyrhizobium-related and formed nitrogen-fixing root nodules on lotus corniculatus and l. pendunculatus, respectively, thus confirming previously defined cross-inoculation groups. rhizobium leguminosarum (rl) strain norway, isolated from l. corni ...	2012	22950721
improvement of drought and salt tolerance in arabidopsis and lotus corniculatus by overexpression of a novel dreb transcription factor from populus euphratica.	a novel dreb (dehydration-responsive element binding) gene, designated pedreb2a, was isolated from the desert-grown tree, populus euphratica oliv. pedreb2a is classified into the a-5 group of dreb subfamily based on multiple sequence alignment and phylogenetic characterization. using semi-quantitative rt-pcr, we found that the pedreb2a was greatly induced by drought, nacl, low temperature, 1-naphthaleneacetic acid (naa), 6-benzyl aminopurine (6-ba) and gibberellic acid (ga3) treatments in p. eup ...	2012	22771912
promex - a mass spectral reference database for plant proteomics.	the promex database is one of the main collection of annotated tryptic peptides in plant proteomics. the main objective of the promex database is to provide experimental ms/ms-based information for cell type-specific or sub-cellular proteomes in arabidopsis thaliana, medicago truncatula, chlamydomonas reinhardtii, lotus japonicus, lotus corniculatus, phaseolus vulgaris, lycopersicon esculentum, solanum tuberosum, nicotiana tabacum, glycine max, zea mays, bradyrhizobium japonicum, and sinorhizobi ...	2012	22685450
an integrated approach to demonstrating the anr pathway of proanthocyanidin biosynthesis in plants.	proanthocyanidins (pas) are oligomers or polymers of plant flavan-3-ols and are important to plant adaptation in extreme environmental conditions. the characterization of anthocyanidin reductase (anr) and leucoanthocyanidin reductase (lar) has demonstrated the different biogenesis of four stereo-configurations of flavan-3-ols. it is important to understand whether anr and the anr pathway widely occur in the plant kingdom. here, we report an integrated approach to demonstrate the anr pathway in p ...	2012	22678031
phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes.	lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ...	2011	22092879
condensed tannins act against cattle nematodes.	the use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (gin) in ruminants. direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat gin. these anthelmintic properties are mainly associated with condensed tannins. in the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine gin. effects of onobrychis viciifolia, lotus pedunculatus and lotus cornic ...	2011	21726942
isolation and characterization of two strains of fusarium oxysporum causing potato dry rot in solanum tuberosum in colombia.	background: fusarium oxysporum has worldwide distribution and causes severe vascular wilt or root rot in many plants. strains are classified into formae speciales based on their high degree of host specificity, of which multilocus sequence typing provides a fairly good estimate. aims: the main aim of this study was to identify the causal agent of an infected potato tuber in colombia. methodology and results: two f. oxysporum isolates were recovered from a potato tuber showing symptoms of dry rot ...	2011	21635960
genetic diversity of root nodule bacteria nodulating lotus corniculatus and anthyllis vulneraria in sweden.	very little is known about the genetic diversity and phylogeny of rhizobia nodulating lotus species in northern temperate regions. we have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from lotus corniculatus and anthyllis vulneraria from different geographic sites and habitats in sweden by restriction fragment length polymorphism (rflp) of the internal transcribed spacer between their 16s rrna and 23s rrna (igs) region. a high diversity consisting of ...	2011	21497473
lotus corniculatus regulates the inflammation induced by bradykinin in a murine model of pleurisy.	this study evaluated the anti-inflammatory efficacy of the crude extract (ce), the fractions derived from hexane (hex), ethyl acetate (acoet), n-butanol (buoh), and aqueous (aq) and isolated compounds (oleanolic acid or kaempferitrin) obtained from the aerial parts of lotus corniculatus var. sa~o gabriel in mice with bradykinin-induced pleurisy. swiss mice were used for the in vivo experiments. inflammatory parameters [leukocytes; exudate concentrations; myeloperoxidase and adenosine-deaminase a ...	2011	21355560
fox-superroots of lotus corniculatus, overexpressing arabidopsis full-length cdna, show stable variations in morphological traits.	using the full-length cdna overexpressor (fox) gene-hunting system, we have generated 130 arabidopsis fox-superroot lines in bird's-foot trefoil (lotus corniculatus) for the systematic functional analysis of genes expressed in roots and for the selection of induced mutants with interesting root growth characteristics. we used the arabidopsis-fox agrobacterium library (constructed by ligating pbig2113sf) for the agrobacterium-mediated transformation of superroots (sr) and the subsequent selection ...	2010	21106274
metabolomics unravel contrasting effects of biodiversity on the performance of individual plant species.	in spite of evidence for positive diversity-productivity relationships increasing plant diversity has highly variable effects on the performance of individual plant species, but the mechanisms behind these differential responses are far from being understood. to gain deeper insights into the physiological responses of individual plant species to increasing plant diversity we performed systematic untargeted metabolite profiling on a number of herbs derived from a grassland biodiversity experiment ...	2010	20830202
two bee-pollinated plant species show higher seed production when grown in gardens compared to arable farmland.	insect pollinator abundance, in particular that of bees, has been shown to be high where there is a super-abundance of floral resources; for example in association with mass-flowering crops and also in gardens where flowering plants are often densely planted. since land management affects pollinator numbers, it is also likely to affect the resultant pollination of plants growing in these habitats. we hypothesised that the seed or fruit set of two plant species, typically pollinated by bumblebees ...	2010	20668704
hydrocarbon degradation and plant colonization by selected bacterial strains isolated from italian ryegrass and birdsfoot trefoil.	to assess the degradation potential and plant colonization capacity of four alkane-degrading strains (itsi10, itri15, itrh76 and btrh79) in combination with birdsfoot trefoil and italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere.	2010	20522148
the effects of condensed tannins extracted from different plant species on egg hatching and larval development of teladorsagia circumcincta (nematoda: trichostrongylidae).	the effects of condensed tannins (cts) extracted from five species of plants on egg hatching and larval development of teladorsagia circumcincta (stadelmann, 1894) (syn. ostertagia circumcincta) were evaluated using in vitro bioassays. the extracts of cts were obtained from lotus pedunculatus (lp), lotus corniculatus (lc), dorycnium pentaphyllum (dp), dorycnium rectum (dr) and rumex obtusifolius (ro). the results of egg hatching assay showed that about 53%, 68%, 51%, 60% and 46% of the eggs hatc ...	2010	20450000
lotus tenuis tolerates combined salinity and waterlogging: maintaining o2 transport to roots and expression of an nhx1-like gene contribute to regulation of na+ transport.	salinity and waterlogging interact to reduce growth for most crop and pasture species. the combination of these stresses often cause a large increase in the rate of na(+) and cl(-) transport to shoots; however, the mechanisms responsible for this are largely unknown. to identify mechanisms contributing to the adverse interaction between salinity and waterlogging, we compared two lotus species with contrasting tolerances when grown under saline (200 mm nacl) and o(2)-deficient (stagnant) treatmen ...	2010	20444189
characterization of strains unlike mesorhizobium loti that nodulate lotus spp. in saline soils of granada, spain.	lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. the aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with lotus spp. a total of 180 isolates able to nodulate lotus corniculatus and lotus tenuis from two locations in granada, spain, were characterized. molecular identificatio ...	2010	20435777
a field study of the effects of elevated co(2) on plant biomass and community structure in a calcareous grassland.	the effects of elevated co(2) on plant biomass and community structure have been studied for four seasons in a calcareous grassland in northwest switzerland. this highly diverse, semi-natural plant community is dominated by the perennial grass bromus erectus and is mown twice a year to maintain species composition. plots of 1.3 m(2) were exposed to ambient or elevated co(2) concentrations (n = 8) using a novel co(2) exposure technique, screen-aided co(2) control (sacc) starting in march 1994. in ...	1999	20135159
454 pyrosequencing based transcriptome analysis of zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides.	an essential driving component in the co-evolution of plants and insects is the ability to produce and handle bioactive compounds. plants produce bioactive natural products for defense, but some insects detoxify and/or sequester the compounds, opening up for new niches with fewer competitors. to study the molecular mechanism behind the co-adaption in plant-insect interactions, we have investigated the interactions between lotus corniculatus and zygaena filipendulae. they both contain cyanogenic ...	2009	19954531
agrobacterium rhizogenes-mediated transformation of superroot-derived lotus corniculatus plants: a valuable tool for functional genomics.	transgenic approaches provide a powerful tool for gene function investigations in plants. however, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. superroot of lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. recently, a technique to obtain transgenic l. corniculatus plants from superroot-derived leaves through a. tu ...	2009	19555486
ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range.	eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ...	2009	19477097
efficacy of multiple applications of oxamyl and phenamiphos for control of pratylenchus penetrans in birdsfoot trefoil.	preplant soil applications of granular phenamiphos effectively reduced pratytenchus penetrans in soil during the seeding year and 1 year after, and in the roots of birdsfoot trefoil 2 years after seeding. forage yields were increased in the season following application of phenamiphos, but stands of plants/m(2) were not greater (p = 0.05) than those in the checks 1 and 2 years after treatment. additional spring applications of phenamiphos 1 and 2 years after seeding further reduced numbers of nem ...	1976	19308245
technical note: effects of forage protein-binding polyphenols on chemistry of dairy excreta.	forage chemistry can affect intake, digestion, milk production, and manure excretion. although information is available on the effects of forage protein-binding polyphenols on small ruminant production and manure excretion, little information is available for dairy cattle. the objective of this study was to compare fecal and urinary n excretion of diets formulated with alfalfa (medicago sativa l.) silage versus condensed tannin-containing birdsfoot trefoil (lotus corniculatus) or o-quinone-conta ...	2009	19307659
functional analysis of three ahl autoinducer synthase genes in mesorhizobium loti reveals the important role of quorum sensing in symbiotic nodulation.	one of the most important signal transduction pathways in bacteria, quorum sensing, is involved in many regulatory circuits in rhizobia, especially in the control of communication between rhizobia and their plant hosts. in this study, we identified 3 autoinducer synthase genes - mrli1, mrli2, and mrli3 - in mesorhizobium loti nzp 2213. we found that mrli1 and mrli2 could synthesize distinct n-acyl homoserine lactone (ahl) autoinducers in rich medium cultures, and the expression of mrli1 was show ...	2009	19295655
additional hosts for the ring nematode, criconemella xenoplax.	some common legumes and weeds indigenous to peach orchards in south carolina were tested in greenhouse experiments to determine their host suitability for criconemella xenoplax. legumes that were hosts for the nematode were dwarf english trefoil (lotus corniculatus var. arvensis), big trefoil (l. uliginosis), birdsfoot trefoil (l. corniculatus), narrowleaf birdsfoot trefoil (l. tenuis), ball clover (trifolium nigrescens), rose clover (t. hirtum), subterranean clover (t. subterraneum), striate le ...	1990	19287692
performance and dietary preferences of white-tailed deer grazing chicory, birdsfoot trefoil or alfalfa in north central alberta.	little information exists on the performance of deer on alternative forage species in northern temperate environments during summer and fall, the period of inherent maximum growth in deer. in performance and choice experiments, we compared live weight gain (g/kg(0.75)/day), absolute [kg/ha dry matter (dm)] and relative (% dm) herbage utilization, relative preference index (rpi) as well as plant community visitation of white-tailed deer grazing alfalfa (medicago sativa), birdsfoot trefoil (lotus ...	2009	19138349
mesorhizobium gobiense sp. nov. and mesorhizobium tarimense sp. nov., isolated from wild legumes growing in desert soils of xinjiang, china.	twenty-four mesorhizobium strains were isolated from desert soils in the xinjiang region of china and were characterized by a polyphasic approach. these strains grouped into three clusters in igs-rflp, sds-page analysis of whole-cell proteins and box-pcr analysis, corresponding to genomic species v, vi and vii as found in a previous study. the results were supported by sequencing analyses of rrs, igs, atpd and reca genes. genospecies vii was most related to mesorhizobium septentrionale, while ge ...	2008	18984702
short-term nutritional treatments grazing legumes or feeding concentrates increase prolificacy in corriedale ewes.	we tested whether short periods of increased nutrition will improve ovulation rate and prolificacy, irrespective of the method used to synchronise the cycles of the ewes. in experiment 1, we used 138 corriedale ewes to evaluate two factors: synchronization treatment (sponges versus a single injection of prostaglandin) and type of pasture (native versus improved with lotus corniculatus). ewes were mated at the end of the grazing period and prolificacy was evaluated at lambing. grazing lotus corni ...	2009	18639397
transgenic superroots of lotus corniculatus can be regenerated from superroot-derived leaves following agrobacterium-mediated transformation.	super-growing roots (superroots; sr), which have been established in the legume species lotus corniculatus, are a fast-growing root culture that allows continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely growth regulator-free culture conditions. these features are unique for non-hairy root cultures, and they are now stably expressed since the culture was isolated more than 10 years ago (1997). attempts to achieve direct and stable transformation o ...	2008	18471930
forage systems for cow-calf production in the appalachian region.	small cow-calf operations are common in the appalachian region. tall fescue [lolium arundinaceum (schreb.) s. j. darbyshire] is the dominant forage in these systems for direct grazing as well as for stockpiling. the present study was conducted from 2001 to 2005. a total of 108 angus and angus crossbred cows were allotted randomly to 6 forage systems and then to 3 replicates within each system. in brief, system 1 had a stocking rate of 0.91 ha/cow in a middleburg 3-paddock (a, b, and c) system. s ...	2008	18407993
alteration of o-specific polysaccharide structure of symbiotically defective mesorhizobium loti mutant 2213.1 derived from strain nzp2213.	mesorhizobium loti mutant 2213.1 derived from the wild-type strain nzp2213 by tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant lotus corniculatus (turska-szewczuk et al., 2007 microbiol res, in press). the inability of lipopolysaccharide (lps) isolated from the mutant 2213.1 strain or de-o-acetylated lps of the parental cells to inactivate phage a1 particles implicated alterations in the lps structure. the o-specific polysaccharide of the mutant was studied by chemi ...	2008	18217107
a high-throughput method for the quantification of proanthocyanidins in forage crops and its application in assessing variation in condensed tannin content in breeding programmes for lotus corniculatus and lotus uliginosus.	lotus corniculatus and lotus uliginosus are agronomically important forage crops used in ruminant livestock production. the condensed tannin (ct) content, dry matter (dm) production, and persistence of these species are key characteristics of interest for future exploitation of these crops. here we present field data on 19 varieties of l. corniculatus, 2 varieties of l. uliginosus and, additionally, a glasshouse experiment using 6 varieties of l. corniculatus and 2 varieties of l. uliginosus. cu ...	2008	18193833
palatability in sheep and in vitro nutritional value of dried and ensiled sainfoin (onobrychis viciifolia) birdsfoot trefoil (lotus corniculatus), and chicory (cichorium intybus).	three temperate forages, sainfoin, birdsfoot trefoil, and chicory, characterized by elevated contents of plant secondary compounds, were compared to a ryegrass-clover mixture (control) in dried (experiment 1) and ensiled form (experiment 2) in their palatability and nutritional value. palatability was measured in adult wethers (n = 6) allowed to choose between the familiar control forage and one of the three test plants. palatability index was calculated from differences in intake of control and ...	2007	18069619
chemotaxis of rhizobium spp. to a glycoprotein produced by birdsfoot trefoil roots.	rhizobium spp. show chemotaxis to plant root exudates. a glycoprotein has been isolated from the root exudates of birdsfoot trefoil, lotus corniculatus, which, at micromolar concentrations, attracts six strains of rhizobia. this glycoprotein has been given the trivial name trefoil chemotactin and contains approximately twice as much protein as carbohydrate. gel filtration of trefoil chemotactin on a bio-gel a-1.5m column gave a molecular weight of approximately 60,000. trefoil chemotactin repres ...	1977	17776952
no-choice preference of cerotoma trifurcata (coleoptera: chrysomelidae) to potential host plants of bean pod mottle virus (comoviridae) in iowa.	to better understand the naturally occurring host range of bean pod mottle virus (family comoviridae, genus comovirus, bpmv) and its principal vector cerotoma trifurcata (förster) (coleoptera: chrysomelidae), 18 field-collected perennial plant species were tested for the presence of bpmv. by using no-choice assays, we determined the preference of these plants by bean leaf beetle, by measuring their level of herbivory relative to soybean, glycine max (l.). new food hosts for adult bean leaf beetl ...	2007	17598542
individual administration of three tanniferous forage plants to lambs artificially infected with haemonchus contortus and cooperia curticei.	we investigated direct anthelmintic effects associated with the feeding of fresh tanniferous forages against established populations of haemonchus contortus and cooperia curticei in lambs. twenty-four parasite naive lambs were inoculated with a single dose of infective larvae of these two parasites 27 days prior to the start of the feeding experiment. lambs were individually fed with either chicory (cichorium intybus), birdsfoot trefoil (lotus corniculatus), sainfoin (onobrychis viciifolia) or a ...	2007	17336459
the incomplete substitution of lipopolysaccharide with o-chain prevents the establishment of effective symbiosis between mesorhizobium loti nzp2213.1 and lotus corniculatus.	mesorhizobium loti nzp2213.1 mutant obtained after random tn5 mutagenesis of m. loti nzp2213 was inefficient in nitrogen fixation on lotus corniculatus. the transposon insertion was located within an orf with a sequence similarity to a putative glycosyl transferase from caulobacter crescentus. the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the mutant produced lps of the same o-chain length but only half of the entire smooth lps, compared to that of the pa ...	2009	17321732
biomass allocation is an important determinant of the tannin concentration in growing plants.	condensed tannins (cts) in the diet affect consumers in a concentration-dependent manner. because of their importance in plant defence against herbivores and pathogens as well as their potential application against gastrointestinal parasites of ruminants in agronomy, an understanding of the seasonal dynamics of ct concentrations during plant growth is essential.	2007	17210606
identification and subcellular localization of the soybean copper p1b-atpase gmhma8 transporter.	we have identified a copper p(1b)-atpase transporter in soybean (glycine max), named as gmhma8, homologue to cyanobacterial pacs and arabidopsis thaliana athma8 (paa2) transporters. a novel specific polyclonal anti-gmhma8 antibody raised against a synthetic peptide reacted with a protein of an apparent mass of around 180-200 kda in chloroplast and thylakoid membrane preparations isolated from soybean cell suspensions. immunoblot analysis with this antibody also showed a band with similar apparen ...	2007	17169574
ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in lotus corniculatus.	proanthocyanidins (pas) are plant secondary metabolites and are composed primarily of catechin and epicatechin units in higher plant species. due to the ability of pas to bind reversibly with plant proteins to improve digestion and reduce bloat, engineering this pathway in leaves is a major goal for forage breeders. here, we report the cloning and expression analysis of anthocyanidin reductase (anr) and leucoanthocyanidin 4-reductase (lar), two genes encoding enzymes committed to epicatechin and ...	2007	17098849
toxicity of phenolic compounds extracted from bark residues of different ages.	in quebec, canada, industrial bark wastelands cover several hundred hectares of land. bark residue that has piled up for decades tends to remain free of vegetation for years. to assess the revegetation potential of such sites, we sought to determine those factors responsible for poor plant growth. phenolic compounds from fresh to 20-year-old bark residues were extracted with four solvents and quantified by high-performance liquid chromatography (hplc). we simulated solutions (mixtures of standar ...	2006	17089184
high sensitivity to auxin is a common feature of hairy root.	the responses to auxin of lycopersicon esculentum roots transformed by (t(l)+t(r))-dna of the ri plasmid of agropine-type agrobacterium rhizogenes strain 15834 and catharanthus trichophyllus roots transformed by the (t(l)+t(r))-dna, and by t(l)- or t(r)- dna alone of the same bacterial strain were compared to that of their normal counterparts. the transmembrane electrical potential difference of root protoplasts was measured as a function of the concentration of exogenous naphthalene acetic acid ...	1990	16667748
hairy roots are more sensitive to auxin than normal roots.	responses to auxin of lotus corniculatus root tips or protoplasts transformed by agrobacterium rhizogenes strains 15834 and 8196 were compared to those of their normal counterparts. three different types of experiments were performed, involving long-term, medium-term, or short-term responses to a synthetic auxin, 1-naphthaleneacetic acid. root tip elongation, proton excretion by root tips, and transmembrane electrical potential difference of root protoplasts were measured as a function of exogen ...	1988	16593928
expression of a complete soybean leghemoglobin gene in root nodules of transgenic lotus corniculatus.	the complete soybean leghemoglobin lbc(3) gene was transferred into the legume lotus corniculatus using an agrobacterium rhizogenes vector system. organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with rhizobium loti. the primary transcript was processed in the same way as in soybean nodules and the resulting mrna was translated into lbc(3) protein. quantitative determination of the lbc(3) protein in nodules of tran ...	1987	16593870
the effects of birdsfoot trefoil (lotus corniculatus) and chicory (cichorium intybus) when compared with perennial ryegrass (lolium perenne) on ovine gastrointestinal parasite development, survival and migration.	two experiments were conducted to investigate the effects of birdsfoot trefoil and chicory on parasitic nematode development, survival and migration when compared with perennial ryegrass. in experiment one, sheep faeces, containing 10,385 cooperia curticei eggs were added to 25 cm diameter pots containing birdsfoot trefoil, chicory or ryegrass, and the pots maintained under optimal conditions for nematode parasite development. replicate pots of each forage type were destructively sampled on day ...	2006	16495015
5' analysis of the soybean leghaemoglobin lbc(3) gene: regulatory elements required for promoter activity and organ specificity.	the soybean leghaemoglobin lbc(3) gene promoter was analysed in transgenic lotus corniculatus plants. hybrid-promoter constructions and 5' deletions were studied using chimeric genes composed of the various promoters, the chloramphenicol acetyltransferase (cat) coding sequence and the lbc(3) 3' flanking region. a 5' bal31 deletion series mapped a strong positive regulatory element between -1100 and -950. a weaker element located between -230 and -170 defined the minimum 5' region required for de ...	1987	16453808
effects of condensed tannins on endoglucanase activity and filter paper digestion by fibrobacter succinogenes s85.	the effect of condensed tannins from birdsfoot trefoil (lotus corniculatus l.) on the cellulolytic rumen bacterium fibrobacter succinogenes s85 was examined. condensed tannins inhibited endoglucanase activity in the extracellular culture fluid, at concentrations as low as 25 mug ml. in contrast, cell-associated endoglucanase activity increased in concentrations of condensed tannins between 100 and 300 mug ml. inhibition of endoglucanase activity in both the extracellular and the cell-associated ...	1993	16348990
n-hexanoyl-l-homoserine lactone, a mediator of bacterial quorum-sensing regulation, exhibits plant-dependent stability and may be inactivated by germinating lotus corniculatus seedlings.	the half-life of n-hexanoyl-l-homoserine lactone (c6-hsl) was determined under various ph and temperature conditions, and in several plant environments. c6-hsl was sensitive to alkaline ph, a process that was also temperature-dependent. in addition, c6-hsl disappeared from plant environments, i.e. axenic monocot and dicot plants cultivated under gnotobiotic, hydroponic conditions, albeit with variable kinetics. the disappearance was rapid at the root system of legume plants such as clover or lot ...	2004	16329888
extra thin alginate film: an efficient technique for protoplast culture.	this paper reports an efficient protoplast culture technique, the "extra thin alginate film" technique. the development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. the efficiency of this technique was evaluated with two model plant systems, viz., nicotiana tabacum and lotus corniculatus, and a comparison was made with the "thin alginate layer" technique, another efficient protoplast cul ...	2005	16244810
herbaceous vegetation productivity, persistence, and metals uptake on a biosolids-amended mine soil.	the selection of plant species is critical for the successful establishment and long-term maintenance of vegetation on reclaimed surface mined soils. a study was conducted to assess the capability of 16 forage grass and legume species in monocultures and mixes to establish and thrive on a reclaimed appalachian surface mine amended with biosolids. the 0.15-ha coarse-textured, rocky, non-acid forming mined site was prepared for planting by grading to a 2% slope and amending sandstone overburden ma ...	2012	16151233
characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose digestion.	to identify simple screening tools for selecting condensed tannin (ct)-containing forages as candidate sources for further study, ct were isolated from nine legumes, and their molecular weights (mw), chromophore production, capacity to precipitate bovine serum albumin (bsa) and fraction 1 protein (rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. sources were as follows: leaves of sericea lespedeza (lespedeza cuneata dum.-cours.), crown vetch (coronilla vari ...	2005	16132212
suspected photosensitisation in lambs grazing birdsfoot trefoil (lotus corniculatus).	suspected photosensitisation occurred in three groups of lambs grazing birdsfoot trefoil (lotus corniculatus c.v. grasslands goldie). in one group, sucking lambs aged about 10 weeks, grazing birdsfoot trefoil, developed skin lesions while lambs of a similar age and from the same flock grazing lucerne (medicago sativa) or a mixed sward of both species showed no signs of photosensitisation. affected lambs had lesions on their backs and ears. in a few animals the tips of the ears were shortened by ...	1995	16031826
de novo alanine synthesis by bacteroids of mesorhizobium loti is not required for nitrogen transfer in the determinate nodules of lotus corniculatus.	deletion of both alanine dehydrogenase genes (alda) in mesorhizobium loti resulted in the loss of alda enzyme activity from cultured bacteria and bacteroids but had no effect on the symbiotic performance of lotus corniculatus plants. thus, neither indeterminate pea nodules nor determinate l. corniculatus nodules export alanine as the sole nitrogen secretion product.	2005	16030244
a comparison of two strategies to modify the hydroxylation of condensed tannin polymers in lotus corniculatus l.	a full-length sense antirrhinum majus dihydroflavonol reductase (dfr) sequence was introduced into birdsfoot trefoil (lotus corniculatus l.) in experiments aimed at modifying condensed tannin content and polymer hydroxylation in a predictable manner. analysis of transgenic plants indicated lines that showed enhanced tannin content in leaf and stem tissues. in contrast to previous data from root cultures, levels of propelargonidin units were not markedly elevated in lines with enhanced tannin con ...	2005	15896367
symbiotic phenotypes and translocated effector proteins of the mesorhizobium loti strain r7a virb/d4 type iv secretion system.	the symbiosis island of mesorhizobium loti strain r7a contains genes with strong similarity to the structural vir genes (virb1-11; vird4) of agrobacterium tumefaciens that encode the type iv secretion system (t4ss) required for t-dna transfer to plants. in contrast, m. loti strain maff303099 lacks these genes but contains genes not present in strain r7a that encode a type iii secretion system (t3ss). here we show by hybridization analysis that most m. loti strains contain the virb/d4 t4ss and no ...	2004	15469524
lotus hairy roots expressing inducible arginine decarboxylase activity.	biotechnological uses of plant cell-tissue culture usually rely on constitutive transgene expression. however, such expression of transgenes may not always be desirable. in those cases, the use of an inducible promoter could be an alternative approach. to test this hypothesis, we developed two binary vectors harboring a stress-inducible promoter from arabidopsis thaliana, driving the beta-glucuronidase reporter gene and the oat arginine decarboxylase. transgenic hairy roots of lotus corniculatus ...	2004	15195973
the effect of dietary forage on the development and survival of helminth parasites in ovine faeces.	studies in new zealand and the uk have shown that lambs grazing birdsfoot trefoil (lotus corniculatus) or chicory (cichorium intybus) have reduced parasite intensities compared to lambs grazing ryegrass swards. however, data in the literature on the influence of forages on helminth parasites is equivocal and the underlying mechanisms by which different forage diets may affect these parasites have not been fully determined. the aim of the experiments reported here was to investigate the hypothesi ...	2003	14651879
effect of flavan-3-ols on in vitro egg hatching, larval development and viability of infective larvae of trichostrongylus colubriformis.	the effects of flavan-3-ols (the monomer units of condensed tannins (ct)) and their galloyl derivatives on the viability of eggs, the development of first stage (l1) larvae, and the viability of the infective larvae of trichostrongylus colubriformis were investigated under in vitro conditions. each of the flavan-3-ol gallates showed some inhibition of egg hatching at 100 microg/ml, and 100% inhibition at 1000 microg/ml, with epigallocatechin gallate being the most effective in the egg hatch (eh) ...	2003	14636684
compost and calcium surface treatment effects on subsoil chemistry in acidic minespoil columns.	surface incorporation of a liming agent in combination with compost or biosolids is a proven way to revegetate acidic minespoils, but little is known about the effect of the surface amendments on subsoil chemistry. we conducted a greenhouse column experiment to investigate how different surface amendments affected plant growth and subsoil chemistry in highly acidic minespoil material. columns were filled with shale minespoil material (ph approximately 2.5), amended with caco3, caso4 x 2h2o (gyps ...	2016	12809279
measuring gene flow from two birdsfoot trefoil (lotus corniculatus) field trials using transgenes as tracer markers.	genetic engineering is becoming a useful tool in the improvement of plants but concern has been expressed about the potential environmental risks of releasing genetically modified (gm) organisms into the environment. attention has focused on pollen dispersal as a major issue in the risk assessment of transgenic crop plants. in this study, pollen-mediated dispersal of transgenes via cross-fertilization was examined. plants of lotus corniculatus l. transformed with either the escherichia coli aspa ...	2003	12755896
the effect of birdsfoot trefoil (lotus corniculatus) and chicory (cichorium intybus) on parasite intensities and performance of lambs naturally infected with helminth parasites.	conventionally, farmers rely upon the routine use of anthelmintics to control helminth parasites and their use has proved highly cost-effective. however, several factors, including the emergence of helminths resistant to pharmaceutical anthelmintics, are forcing farmers to seek alternative approaches to parasite control. studies in new zealand have shown that some alternative forages may reduce parasitic infestation in sheep. in the current study, it was found that under uk environmental conditi ...	2003	12581592
lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep.	condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. however, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. the aim of this study was to investigate the specific effects of condensed tannins from lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (lolium perenne ...	2002	12489781
oxygen-induced membrane depolarizations in legume root nodules (possible evidence for an osmoelectrical mechanism controlling nodule gas permeability).	various stresses trigger rapid and reversible decreases in the o2 permeability (po) of legume root nodules. several possible mechanisms have been proposed, but no supporting data have previously been presented that meet the requirements for both rapidity and reversibility. stomatal regulation of gas permeability in leaves involves electrically driven fluxes of inorganic osmoticants, so we investigated the possibility of a somewhat similar mechanism in nodules. we used microelectrodes to monitor ...	1995	12228468
nitrate effects on nodule oxygen permeability and leghemoglobin (nodule oximetry and computer modeling).	two current hypotheses to explain nitrate inhibition of nodule function both involve decreased o2 supply for respiration in support of n2 fixation. this decrease could result from either (a) decreased o2 permeability (po) of the nodule cortex, or (b) conversion of leghemoglobin (lb) to an inactive, nitrosyl form. these hypotheses were tested using alfalfa (medicago sativa l. cv weevlchek) and birdsfoot trefoil (lotus corniculatus l. cv fergus) plants grown in growth pouches under controlled cond ...	1995	12228439
performance of steers grazing rhizomatous and nonrhizomatous birdsfoot trefoil in pure stands and in tall fescue mixtures.	this study investigated the performance of steers grazing rhizomatous birdsfoot trefoil (lotus corniculatus l.) (rbft) compared to nonrhizomatous birdsfoot trefoil (bft) in pure stands or when interseeded with endophyte-free tall fescue (festuca arundinacea schreb.; tf). five forage treatments of rbft, bft, tf, rbft+tf, and bft+tf (four replicate paddocks per treatment) were continuously stocked in spring and fall of 1998 and spring of 1999. grazing for individual treatments was terminated when ...	2002	12162667
the effect of diet fed to lambs on subsequent development of trichostrongylus colubriformis larvae in vitro and on pasture.	contrasting herbage diets were fed to lambs to evaluate their effect on subsequent development of trichostrongylus colubriformis larvae in faeces and on pasture. the diets had either no condensed tannin (ct), lucerne (medicago sativa cv. otaio), white clover (trifolium repens cv. tahora), or had moderate to high concentrations of ct, sulla (hedysarum coronarium cv. grassland aokau), lotus corniculatus (cv. grasslands goldie), l. pedunculatus (cv. grassland maku), dorycnium pentophyllum, and dory ...	2002	11983303
effect of condensed tannins on egg hatching and larval development of trichostrongylus colubriformis in vitro.	the effects of condensed tannins extracted from seven forages on the viability of the eggs and first stage (l1) larvae of the sheep nematode trichostrongylus colubriformis were evaluated in in vitro assays. the extracts of condensed tannins were obtained from lotus pedunculatus (lp), lotus corniculatus (lc), sulla (hedysarum coronarium), sainfoin (onobrychus viciifolia), dorycnium pentaphylum (dp), dorycnium rectum (dr) and dock (rumex obtusifolius). extracts containing 200 to 500 microg/ml redu ...	2002	11837588
the root-nodule symbiosis between sarothamnus scoparius l. and its microsymbionts.	when nitrogen fixing root nodules are formed, sarothamnus scoparius (broom) is inoculated with its microsymbionts. nodules studied under light and electron microscopy exhibited typical indeterminate nodule histology with apical, persistent meristem, age gradient of nodule tissues, and open vascular bundles, and also with some particular features such as: the presence of mitotic activity in the infected meristematic cells, lack of infection threads, distribution of bacteria by process of host cel ...	2001	11816984