Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
[a new cytotoxic isocoumarin from endophytic fungus aspergillus versicolor].a new isocoumarin, along with five known ones,were isolated from the fermentation products of an endophytic fungus aspergillus versicolorby using various chromatographic techniques.their structures were elucidated on the basis of extensivespectroscopic analysis, including 1d-and 2d-nmr techniques. compound 1 was evaluated for cytotoxicity against five human tumor cell lines. the results showed that 1 exhibited weak cytotoxicityagainst nb4, shsy5y and mcf7 cells with ic₅₀ values of 6.8, 4.3,8.8 μ ...201628845647
ecology and identification of environmental fungi and metabolic processes involved in the biodeterioration of brazilian soapstone historical monuments.the present study aimed to evaluate the action of organic acids produced by the fungal population associated with the biodeterioration process of the twelve prophets of aleijadinho, a set of soapstone sculptures in congonhas, state of minas gerais, brazil. for this, samples of fungi were obtained from the surface of each of the twelve outdoor stone sculptures that comprise the set of prophets. identification of the colonizing filamentous fungi was performed by classical microbiology and molecula ...201728833313
aerosolization of mycotoxins after growth of toxinogenic fungi on wallpaper.many fungi can develop on building material in indoor environments if moisture is high enough. among species that are frequently observed, some are known to be potent mycotoxin producers. this presence of toxinogenic fungi in indoor environments raises the question of the possible exposure of occupants to these toxic compounds by inhalation after aerosolization.this study investigated the mycotoxin production by penicillium brevicompactum, aspergillus versicolor and stachybotrys chartarum during ...201728646113
antifungal and antiviral cyclic peptides from the deep-sea-derived fungus simplicillium obclavatum eiodsf 020.a new linear peptide simplicilliumtide i (1) and four new cyclic peptides simplicilliumtides j-m (2-5) together with known analogues verlamelins a and b (6 and 7) were isolated from the deep-sea-derived fungal strain simplicillium obclavatum eiodsf 020. their structures were elucidated by spectroscopic analysis, and their absolute configurations were further confirmed by chemical structural modification, marfey's and mosher's methods. compounds 2, 6, and 7 showed significant antifungal activity ...201728578573
antimicrobial activity and molecular docking studies of a novel anthraquinone from a marine-derived fungus aspergillus versicolor.a novel anthraquinone, 2-(dimethoxymethyl)-1-hydroxyanthracene-9,10-dione (1), together with nine known compounds (2-10), were isolated from the fermentation of aspergillus versicolor derived from deep sea sediment. their structures were established through spectroscopic methods. compound 1 exhibited strong inhibitory activities against mrsa atcc 43300 and mrsa cgmcc 1.12409 (with mic values of 3.9 and 7.8 μg/ml respectively) and moderate activities against tested strains of vibrio (with mic val ...201728511613
free chlorine and monochloramine inactivation kinetics of aspergillus and penicillium in drinking water.fungi are near-ubiquitous in potable water distribution systems, but the disinfection kinetics of commonly identified fungi are poorly studied. in the present study, laboratory scale experiments were conducted to evaluate the inactivation kinetics of aspergillus fumigatus, aspergillus versicolor, and penicillium purpurogenum by free chlorine and monochloramine. the observed inactivation data were then fit to a delayed chick-watson model. based on the model parameter estimation, the ct values (in ...201728501787
contribution of filamentous fungi to the musty odorant 2,4,6-trichloroanisole in water supply reservoirs and associated drinking water treatment plants.in this study, the distribution of 2,4,6-trichloroanisole (2,4,6-tca) in two water supply reservoirs and four associated drinking water treatment plants (dwtps) were investigated. the 2,4,6-tca concentrations were in the range of 1.53-2.36 ng l(-1) in water supply reservoirs and 0.76-6.58 ng l(-1) at dwtps. to determine the contribution of filamentous fungi to 2,4,6-tca in a full-scale treatment process, the concentrations of 2,4,6-tca in raw water, settled water, post-filtration water, and fini ...201728499183
antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide.brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ...201728407186
discrimination of three genetically close aspergillus species by using high resolution melting analysis applied to indoor air as case study.indoor air pollution caused by fungal contamination is suspected to have a public health impact. monitoring of the composition of the indoor airborne fungal contaminants is therefore important. to avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. among these molecular methods, the polymerase chain reaction (pcr) and the real-time pcr are the most frequently used tools for indoor fungal detection. however, even if these tool ...201728376723
investigation of inflammatory and allergic responses to common mold species: results from in vitro experiments, from a mouse model of asthma, and from a group of asthmatic patients.most studies on molds focus on alternaria alternata and aspergillus fumigatus. here, we report on inflammatory and allergenic properties of more typical indoor species aspergillus versicolor, p. chrysogenum, c. cladosporioïdes, and c. sphaerospermum that were compared to a. alternata and a. fumigatus. in a mouse model, after intranasal instillation, a. alternaria, a. versicolor, and c. sphaerospermum induced the early recruitment of neutrophils and the strong expression of inflammatory markers i ...201728370571
fungal biodiversity and mycotoxigenic fungi in cooling-tower water systems in istanbul, turkey.this is the first study to assess fungal diversity and mycotoxigenic fungi in open recirculating cooling-tower (ct) water systems (biofilm and water phase). the production capability of mycotoxin from fungal isolates was also examined. the mean fungal count in 21 different water and biofilm samples was determined as 234 cfu/100 ml and 4 cfu/cm(2). a total of 32 species were identified by internal transcribed spacer (its) sequencing. the most common isolated fungi belonged to the genera aspergill ...201728362312
endophytic fungal isolate mediated biosynthesis of silver nanoparticles and their free radical scavenging activity and anti microbial studies.the present study reports that the biosynthesis of agnps using an endophytic fungus isolated from the ethnomedicinal plant centella asiatica. the endophytic fungus was identified as aspergillus versicolor ent7 based on 18s rrna gene sequencing (ncbi accession number kf493864). the agnps synthesized were characterized by uv-visible spectroscopy, fourier transform infra-red spectroscopy (ftir), transmission electron microscopy (tem), x-ray diffraction (xrd), particle size analyzer, and zeta potent ...201628330204
development of a novel compound microbial agent for degradation of kitchen waste.large quantities of kitchen waste are produced in modern society and its disposal poses serious environmental and social problems. the aim of this study was to isolate degradative strains from kitchen waste and to develop a novel and effective microbial agent. one hundred and four strains were isolated from kitchen waste and the 84 dominant strains were used to inoculate protein-, starch-, fat- and cellulose-containing media for detecting their degradability. twelve dominant strains of various s ...201728279600
anti-hsv-1, antioxidant and antifouling phenolic compounds from the deep-sea-derived fungus aspergillus versicolor scsio 41502.chemical investigation of the deep-sea-derived fungus aspergillus versicolor scsio 41502 resulted in the isolation of three new anthraquinones, aspergilols g-i (1-3), one new diphenyl ether, 4-carbglyceryl-3,3'-dihydroxy-5,5'-dimethyldiphenyl ether (4), and one new benzaldehyde derivative, 2,4-dihydroxy-6-(4-methoxy-2-oxopentyl)-3-methylbenzaldehyde (5), along with 23 known phenolic compounds (6-28). the structures of new compounds were elucidated by extensive spectroscopic analysis. the absolut ...201728129981
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens.filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear.201728100314
new compounds from a hydrothermal vent crab-associated fungus aspergillus versicolor xz-4.three new quinazoline derivatives (1-3), one new oxepin-containing natural product (4) and four new cyclopenin derivatives (5-7 and 9) have been isolated from an etoac extract of the taiwan kueishantao hydrothermal vent crab-associated fungus aspergillus versicolor xz-4. their planar structures were established by hrms, 1d and 2d nmr spectroscopic data analyses. the absolute configurations for compounds 1 and 4 were determined by chiral phase hplc analysis of their hydrolysis products. the absol ...201728074949
the pygidial gland secretion of the forest caterpillar hunter, calosoma (calosoma) sycophanta: the antimicrobial properties against human pathogens.recently, various insect secretions have been tested as possible antimicrobial agents. in beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder adephaga. the antimicrobial potential of the pygidial gland secretion isolated from the adults of calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe c ...201728070663
enzymatic activity of fungi isolated from crops.to detect and assess the activity of extracellular hydrolytic enzymes and to find differences in enzymograms between fungi isolated from wheat and rye samples and grown on czapek-dox broth and sabouraud dextrose broth enriched with cereal (wheat or rye). isolated strains were also classified in the scale of biosafety levels (bsl).201628035224
modeling growth and toxin production of toxigenic fungi signaled in cheese under different temperature and water activity regimes.the aim of this study was to investigate in vitro and model the effect of temperature (t) and water activity (aw) conditions on growth and toxin production by some toxigenic fungi signaled in cheese. aspergillus versicolor, penicillium camemberti, p. citrinum, p. crustosum, p. nalgiovense, p. nordicum, p. roqueforti, p. verrucosum were considered they were grown under different t (0-40 °c) and aw (0.78-0.99) regimes. the highest relative growth occurred around 25 °c; all the fungi were very susc ...201628029129
assessment of multi-contaminant exposure in a cancer treatment center: a 2-year monitoring of molds, mycotoxins, endotoxins, and glucans in bioaerosols.indoor air quality in health care facilities is a major public health concern, particularly for immunocompromised patients who may be exposed to microbiological contaminants such as molds, mycotoxins, endotoxins, and (1,3)-ß-d-glucans. over 2 years, bioaerosols were collected on a monthly basis in a cancer treatment center (centre f. baclesse, normandy, france), characterized from areas where there was no any particular air treatment. results showed the complexity of mycoflora in bioaerosols wit ...201728012082
versiquinazolines a-k, fumiquinazoline-type alkaloids from the gorgonian-derived fungus aspergillus versicolor lzd-14-1.eleven fumiquinazoline-type alkaloids, namely, versiquinazolines a-k (1-11), along with cottoquinazolines b-d, were isolated from the gorgonian-derived fungus aspergillus versicolor lzd-14-1. their structures were determined by extensive analyses of the spectroscopic data (1d and 2d nmr, hresims), in addition to the experimental and calculated ecd data and x-ray single-crystal diffraction analysis for the assignments of the absolute configurations. versiquinazolines a, b, and f (1, 2, and 6), be ...201627933898
development of a dot-blot assay for the detection of mould-specific ige in the belgian population.data on mould sensitization in the general population are scarce and mostly on aspergillus fumigatus, alternaria alternata and cladosporium herbarum.201727878528
respiratory symptoms and fractional exhaled nitric oxide (feno) among students in penang, malaysia in relation to signs of dampness at school and fungal dna in school dust.few health studies exist on dampness and mould in schools in the tropics. we studied associations between fraction of exhaled nitric oxide (feno), respiratory symptoms and airway infections among students and dampness and fungal dna in schools in malaysia. a total of 368 randomly selected students from 32 classrooms in 8 secondary schools in penang, malaysia, participated (58% participation rate). information on current respiratory symptoms and the home environment was collected by a standardise ...201727802882
arabidopsis thaliana as bioindicator of fungal vocs in indoor air.in this paper, we demonstrate the ability of arabidopsis thaliana to detect different mixtures of volatile organic compounds (vocs) emitted by the common indoor fungus, aspergillus versicolor, and demonstrate the potential usage of the plant as a bioindicator to monitor fungal vocs in indoor air. we evaluated the volatile production of aspergillus versicolor strains srrc 108 (nrrl 3449) and srrc 2559 (atcc 32662) grown on nutrient rich fungal medium, and grown under conditions to mimic the subst ...201627790067
species diversity of aspergillus section versicolores in clinical samples and antifungal susceptibility.aspergillus section versicolores includes species of clinical relevance and many others that have been poorly studied but are occasionally found in clinical samples. the aim of this study was to investigate, using a multilocus phylogenetic approach, the spectrum of species of the section versicolores and to determine their in vitro antifungal susceptibility. the study was based on a set of 77 clinical isolates from different usa medical centres, which had been previously identified as belonging ...201627742099
aspernolides l and m, new butyrolactones from the endophytic fungus aspergillus versicolor.during the systematic search of active compounds from endophytic fungi, two new butyrolactones, namely aspernolides l (2) and m (4), together with four known compounds: 1-o-acetylglycerol (1), butyrolactone i (3), butyrolactone vi (5), and (+) alantrypinone (6) were characterized from the etoac extract of the endophytic fungus aspergillus versicolor isolated from the roots of pulicaria crispa (asteraceae). extensive spectroscopic analysis, including 1d, 2d nmr, and hresims, was used to elucidate ...201627658145
pereskia aculeata muller (cactaceae) leaves: chemical composition and biological activities.the aims of this work were to study the chemical composition of the essential oil from the leaves of pereskia aculeata and to evaluate some biological activities of three leaf extracts. the phenolic content, antioxidant activity, and in vitro antimicrobial and antifungal activities were determined. the methanol extract showed antioxidant activity (ec50 7.09 mg/ml) and high polyphenols content (15.04 ± 0.31 mg gallic acid equivalents (gae)/g). the petroleum ether extract exhibited potent antibact ...201627598154
aspergillus fumigatus carrying tr34/l98h resistance allele causing complicated suppurative otitis media in tanzania: call for improved diagnosis of fungi in sub-saharan africa.suppurative otitis media (som) is a major public health concern worldwide and is associated with increased morbidity. cases of fungal suppurative otitis media were studied to establish the effect of fungi in otitis media.201627589956
occurrence of the toxin-producing aspergillus versicolor tiraboschi in residential buildings.in an area representative of a moderate climate zone (lubuskie province in poland), mycological tests in over 270 flats demonstrated the occurrence of 82 species of moulds. aspergillus versicolor tiraboschi was often encountered on building partitions (frequency 4: frequently). the ability to synthesize the carcinogenic sterigmatocystin (st) means that it poses a risk to humans and animals. biotoxicological tests of biomasses of a. versicolor were conducted in the microbiological and toxicologic ...201627589778
the capability of fungi isolated from moldy dwellings to produce toxins.the main objective was analysis and assessment of toxinogenic capabilities of fungi isolated from moldy surfaces in residential rooms in an urban agglomeration situated far from flooded areas in moderate climate zone.201627518891
versicones e-h and arugosin k produced by the mangrove-derived fungus aspergillus versicolor hdn11-84.four new xanthonoids, versicones e-h (1-4), and a biogenetically related new derivative arugosin k (5), were isolated from a culture extract of the mangrove-derived fungus aspergillus versicolor hdn11-84. their structures were elucidated on the basis of extensive nmr spectroscopic data analysis. versicone e (1) represents the first example of naturally occurring xanthonoids containing a 2-butenamide moiety. among them, 5 showed the best cytotoxicity against hela cell line with an ic50 value of 9 ...201727460761
synthesis of psychrophilin e.the first total synthesis of psychrophilin e, a potent antiproliferative cyclic tripeptide isolated from aspergillus versicolor zln-60, is reported herein. key features of the synthesis include the installation of an amide bond between the indole-nitrogen of tryptophan and an anthranilic acid residue, and a high yielding macrolactamization of the linear tripeptide to the desired macrocycle.201627442351
biochemical characterization and antimicrobial and antifungal activity of two endemic varieties of garlic (allium sativum l.) of the campania region, southern italy.extracts of the bulbs of the two endemic varieties "rosato" and "caposele" of allium sativum of the campania region, southern italy, were analyzed. the phenolic content, ascorbic acid, allicin content, and in vitro antimicrobial and antifungal activity were determined. ultra performance liquid chromatography with diode array detector performed polyphenol profile. the polyphenolic extracts showed antioxidant activity (ec50) lower than 120 mg. the amount of ascorbic acid and allicin in the two ext ...201627259073
pharmacokinetic/pharmacodynamic analysis of voriconazole against candida spp. and aspergillus spp. in children, adolescents and adults by monte carlo simulation.the objective of this study was to investigate the cumulative fraction of response of various voriconazole dosing regimens against six candida and six aspergillus spp. in immunocompromised children, immunocompromised adolescents, and adults. using pharmacokinetic parameters and pharmacodynamic data, 5000-subject monte carlo simulations (mcss) were conducted to evaluate the ability of simulated dosing strategies in terms of fauc/mic targets of voriconazole. according to the results of the mcss, c ...201627179818
extracellular polysaccharide with novel structure and antioxidant property produced by the deep-sea fungus aspergillus versicolor n2bc.an extracellular polysaccharide, n1, was obtained from the culture medium of the deep-sea fungus aspergillus versicolor n2bc by a combination of ethanol precipitation, ion-exchange and gel filtration chromatography. n1 was a mannoglucogalactan with molecular weight of about 20.5kda. results of chemical and spectroscopic analyses, including fourier-transform infrared, one- and two-dimensional nuclear magnetic resonance spectroscopy showed that the main chain of n1 consisted of →2)-α-d-glcp-(1→, → ...201627178933
how to diagnose mould allergy? comparison of skin prick tests with specific ige results.diagnosis of mould allergy is complicated due to the heterogeneity of the test material and the decrease in the number of commercial mould skin test solutions that are currently available.201627027397
microbial contamination level of air in animal waste utilization plants.the aim of this research was evaluation of microbial contamination of air within and in the vicinity of animal waste disposal plants.201627007517
antifungal new oxepine-containing alkaloids and xanthones from the deep-sea-derived fungus aspergillus versicolor scsio 05879.phytopathogenic fungi remain a continuous and huge threat in the agricultural fields. the agrochemical industry has made great development of the use of microbial natural products, which has been regarded as an effective strategy against phytopathogenic fungi. antifungal bioassay-directed fractionation was used to isolate two new oxepine-containing alkaloids (1 and 2), two new 4-aryl-quinolin-2-one alkaloids (3 and 4), and four new prenylated xanthones (5-8) from the deep-sea-derived fungus aspe ...201626998701
a biochar-based medium in the biofiltration system: removal efficiency, microorganism propagation, and the medium penetration modeling.biofiltration is a method of biological treatment belonging to cleaner technologies because it does not produce secondary air pollutants, but helps to integrate natural processes in microorganisms for decomposing volatile air pollutants and solving odor problems. the birch wood biochar has been chosen as a principal material for biofilter bed medium. the experiments were conducted at the temperatures of 24, 28, and 32 °c, while the concentration of acetone, xylene, and ammonium reached 300 mg/m( ...201626980677
generation and characterization of indoor fungal aerosols for inhalation studies.in the indoor environment, people are exposed to several fungal species. evident dampness is associated with increased respiratory symptoms. to examine the immune responses associated with fungal exposure, mice are often exposed to a single species grown on an agar medium. the aim of this study was to develop an inhalation exposure system to be able to examine responses in mice exposed to mixed fungal species aerosolized from fungus-infested building materials. indoor airborne fungi were sampled ...201626921421
bioactivity, proximate, mineral and volatile profiles along the flowering stages of opuntia microdasys (lehm.): defining potential applications.opuntia spp. flowers have been traditionally used for medical purposes, mostly because of their diversity in bioactive molecules with health promoting properties. the proximate, mineral and volatile compound profiles, together with the cytotoxic and antimicrobial properties were characterized in o. microdasys flowers at different maturity stages, revealing several statistically significant differences. o. microdasys stood out mainly for its high contents of dietary fiber, potassium and camphor, ...201626876160
rhinitis, ocular, throat and dermal symptoms, headache and tiredness among students in schools from johor bahru, malaysia: associations with fungal dna and mycotoxins in classroom dust.there are few studies on rhinitis and sick building syndrome (sbs) among students in tropical countries. we studied associations between levels of five fungal dna sequences, two mycotoxins (sterigmatocystin and verrucarol) and cat allergen (fel d 1) levels in schools and rhinitis and other weekly sbs symptoms in the students. fungal dna was measured by quantitative pcr and cat allergen by elisa. pupils (n = 462) from eight randomly selected schools in johor bahru, malaysia participated (96%). du ...201626829324
release and characteristics of fungal fragments in various conditions.intact spores and submicrometer size fragments are released from moldy building materials during growth and sporulation. it is unclear whether all fragments originate from fungal growth or if small pieces of building materials are also aerosolized as a result of microbial decomposition. in addition, particles may be formed through nucleation from secondary metabolites of fungi, such as microbial volatile organic compounds (mvocs). in this study, we used the elemental composition of particles to ...201626789361
antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.201726729960
exploring the antibacterial and antifungal potential of jellyfish-associated marine fungi by cultivation-dependent approaches.fungi isolated from marine invertebrates are of considerable importance as new promising sources of unique secondary metabolites with significant biomedical potential. however, the cultivable fungal community harbored in jellyfish was less investigated. in this work, we seek to recover symbiotic fungi from different tissues of jellyfish nemopilema nomurai. a total of seven morphotypes were isolated, which were assigned into four genera (aspergillus, cladosporium, purpureocillium, and tilletiopsi ...201526637162
avertoxins a-d, prenyl asteltoxin derivatives from aspergillus versicolor y10, an endophytic fungus of huperzia serrata.aspergillus versicolor y10 is an endophytic fungus isolated from huperzia serrata, which showed inhibitory activity against acetylcholinesterase. an investigation of the chemical constituents of y10 led to the isolation of four new prenylated asteltoxin derivatives, named avertoxins a-d (2-5), together with the known mycotoxin asteltoxin (1). in the present study, we report structure elucidation for 2-5 and the revised nmr assignments for asteltoxin and demonstrated that avertoxin b (3) is an ac ...201526618211
effect of water activity and titania p25 photocatalyst on inactivation of pathogenic fungi--contribution to the protection of public health.the aim of this study was to determine the antifungal activity of titanium dioxide activated by indoor light on czapek yeast agar (cya).201526615661
detection of boron removal capacities of different microorganisms in wastewater and effective removal process.in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ...201526540546
versixanthones a-f, cytotoxic xanthone-chromanone dimers from the marine-derived fungus aspergillus versicolor hdn1009.six unusual xanthone-chromanone dimers, versixanthones a-f (1-6), featuring different formal linkages of tetrahydroxanthone and 2,2-disubstituted chroman-4-one monomers, were isolated from a culture of the mangrove-derived fungus aspergillus versicolor hdn1009. the absolute configurations of 1-6, representing the central and axial chirality elements or preferred helicities, were established by a combination of x-ray diffraction analysis, chemical conversions, and tddft-ecd calculations. the inte ...201526506221
efficient biotransformation of ginsenoside rb1 to rd by isolated aspergillus versicolor, excreting β-glucosidase in the spore production phase of solid culture.ginsenosides are the major pharmacological components in ginseng. microorganisms from a ginseng field were isolated to identify transformation of ginsenosides. based on hplc and lc-ms analysis, strain lfj1403 showed strong activities to transform ginsenoside rb1 to rd as the sole product. phylogenetic analysis of 18s rdna indicated that lfj1403 belonged to aspergillus versicolor. through comparing four systems of transforming rb1 to rd, strain lfj1403 was found to secrete ginsenoside-converting ...201526373416
development of the dichlorvos-ammonia (dv-am) method for the visual detection of aflatoxigenic fungi.aflatoxins (afs) are carcinogenic and toxic secondary metabolites produced mainly by aspergillus flavus and aspergillus parasiticus. to monitor and regulate the af contamination of crops, a sensitive and precise detection method for these toxigenic fungi in environments is necessary. we herein developed a novel visual detection method, the dichlorvos-ammonia (dv-am) method, for identifying af-producing fungi using dv and am vapor on agar culture plates, in which dv inhibits the esterase in af bi ...201526300294
microbiological consequences of indoor composting.recycling of organic waste appeals to more and more people. the aim of this study was to evaluate the microbiological contamination around organic waste bins at three distances over a 12-month period. contamination near the customary trash of control households was evaluated at the beginning to ensure that there is no recruitment bias. air samples using the mas 100 impactor were carried out in 38 dwellings that do household waste composting and in 10 dwellings of controls. collection of particle ...201626299932
erratum to: development and performance assessment of a qualitative sybr® green real-time pcr assay for the detection of aspergillus versicolor in indoor air. 201526293339
aspergillus in endodontic infection near the maxillary sinus.diseases of the maxillary sinus have been associated with dental roots near the maxillary sinus that have undergone endodontic treatment.201526254203
development and performance assessment of a qualitative sybr® green real-time pcr assay for the detection of aspergillus versicolor in indoor air.currently, contamination of indoor environment by fungi and molds is considered as a public health problem. the monitoring of indoor airborne fungal contamination is a common tool to help understanding the link between fungi in houses and respiratory problems. classical analytical monitoring methods, based on cultivation and microscopic identification, depend on the growth of the fungi. consequently, they are biased by difficulties to grow some species on certain culture media and under certain ...201526184975
fungal dna in dust in swedish day care centres: associations with respiratory symptoms, fractional exhaled nitrogen oxide (feno) and c-reactive protein (crp) in serum among day care centre staff.to study associations between fungal dna in day care centres, fractional exhaled nitric oxide (feno) and inflammatory markers in day care centre staff.201626183154
the effect of anatase and rutile crystallites isolated from titania p25 photocatalyst on growth of selected mould fungi.antifungal properties of anatase and rutile crystallites isolated from commercial titania p25 photocatalyst were investigated by mycelium growth in the dark and under indoor light. investigated fungi, i.e., pseudallescheria boydii, scedosporium apiospermum, pseudallescheria ellipsoidea, scedosporium aurantiacum, aspergillus versicolor, aspergillus flavus, stachybotrys chartarum, penicillium chrysogenum, aspergillus melleus, were isolated from air and from moisture condensed on walls. anatase and ...201526177513
selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting.the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ...201426139877
indirect immunodetection of fungal fragments by field emission scanning electron microscopy.submicronic fungal fragments have been observed in in vitro aerosolization experiments. the occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. however, the role of submicronic fragments in exacerbating adverse health effects has remained unclear due to limitations associated with detection methods. in the present study, we report the development of an indirect immunodetection assay that utiliz ...201526092450
sterigmatocystin presence in typical latvian grains.ninety five samples of different latvian grains (wheat, buckwheat, barley, oats and rye) from the year 2006 and 120 samples from the year 2007 were analyzed for aspergillus ssp. mycotoxin-sterigmatocystin (stc) content. 13.7% of the analyzed 2006 year samples were positive for stc with the concentration levels ranging from <0.7 to 83μg/kg and 35% of the analyzed 2007 year samples were positive for stc with the concentration levels ranging from <1 to 47μg/kg. a previously developed sensitive lc - ...200826054287
maldi-tof mass spectrometry fingerprinting: a diagnostic tool to differentiate dematiaceous fungi stachybotrys chartarum and stachybotrys chlorohalonata.stachybotrys chartarum and stachybotrys chlorohalonata are two closely related species. unambiguous identification of these two species is a challenging task if relying solely on morphological criteria and therefore smarter and less labor-intensive approaches are needed. here we show that even such closely related species of fungi as s. chartarum and s. chlorohalonata are unequivocally discriminated by their highly reproducible maldi-tof-ms fingerprints (matrix assisted laser desorption/ionizati ...201526036596
fungi in ontario maple syrup & some factors that determine the presence of mold damage.maple syrup is a high value artisanal product produced mainly in canada and a number of states primarily in the northeast usa. mold growth (wallemia sebi) on commercial product was first reported in syrup in 1908. since then, few data have been published. we conducted a systematic examination for fungi in maple syrup from 68 producers from all of the syrup-producing areas of ontario, canada. the mean ph of the samples was ph 6.82, sugar content averaged 68.0±0.89 °brix and aw averaged 0.841±0.01 ...201526001061
identification and biological evaluation of secondary metabolites from the endolichenic fungus aspergillus versicolor.a chemical investigation of the endolichenic fungus aspergillus versicolor (125a), which was found in the lichen lobaria quercizans, resulted in the isolation of four novel diphenyl ethers, named diorcinols f-h (1-3, resp.) and 3-methoxyviolaceol-ii (4), eight new bisabolane sesquiterpenoids, named (-)-(r)-cyclo-hydroxysydonic acid (5), (-)-(7s,8r)-8-hydroxysydowic acid (6), (-)-(7r,10s)-10-hydroxysydowic acid (7), (-)-(7r,10r)-iso-10-hydroxysydowic acid (8), (-)-12-acetoxy-1-deoxysydonic acid ( ...201525879502
versicorin, a new lovastatin analogue from the fungus aspergillus versicolor sc0156.a new lovastatin analogue versicorin (1), together with three related compounds, decumbenones a (2) and b (3) and versiol (4), were isolated from mycelial solid cultures of aspergillus versicolor sc0156. their structures were elucidated on the basis of ms data and nmr spectroscopic analysis. the new compound versicorin (1) possesses a hexahydro-2h-naphtho[1,8-bc]furan moiety, which is a rare type of the lovastatin-analogous compounds. a hypothetical biosynthetic pathway for compounds 1-4 was pro ...201525835225
biodegradation of pesticide triclosan by a. versicolor in simulated wastewater and semi-synthetic media.triclosan is known as an antimicrobial agent, a powerful bacteriostat and an important pesticide. in this paper biodegradation of triclosan by aspergillus versicolor was investigated. effects of simulated wastewater and semi-synthetic media on fungal triclosan degradation process were detected. hplc analysis showed that fungal triclosan biodegradation yield was 71.91% at about 7.5 mg/l concentration in semi-synthetic medium and was 37.47% in simulated wastewater. fungus could be able to tolerate ...201525752427
versicolols a and b, two new prenylated isocoumarins from endophytic fungus aspergillus versicolor and their cytotoxic activity.versicolols a and b (1 and 2), two rare prenylated isocoumarin derivatives, along with five known isocoumarins (3-7) were isolated from the fermentation products of an endophytic fungus aspergillus versicolor. their structures were elucidated on the basis of extensive spectroscopic analysis, including 1d- and 2d-nmr techniques. compounds 1 and 2 were evaluated for their cytotoxicity against five human tumor cell lines. the results showed that compounds 1 exhibited weak cytotoxicity against a549 ...201725749845
antiviral butyrolactones from the endophytic fungus aspergillus versicolor.versicolactones a-d (1-4), four new butyrolactones, along with four known butyrolactones (5-8) were isolated from the fermentation products of the endophytic fungus aspergillus versicolor. the structures of compounds 1-4, including absolute configuration, were elucidated by interpretation of the nmr and cd data. compound 2 was further confirmed by single-crystal x-ray diffraction analysis. in particular, compound 1 is the first naturally occurring butyrolactone possessing an unusual 2-oxopropyl ...201525590371
effects of fungal species, cultivation time, growth substrate, and air exposure velocity on the fluorescence properties of airborne fungal spores.real-time bioaerosol monitoring is possible with fluorescence based instruments. this study provides information on major factors that can affect the fluorescence properties of airborne fungal spores. two fluorescence-based bioaerosol detectors, bioscout, and ultraviolet aerodynamic particle sizer (uvaps), were used to study fluorescent particle fractions (fpfs) of released spores of three fungal species (aspergillus versicolor, cladosporium cladosporioides, and penicillium brevicompactum). two ...201525292152
quantification of c. globosum spores in house dust samples.chaetomium globosum is one of the most common fungi that grows in damp buildings and occurs in agricultural and forestry workplaces. using sera from atopic patients, we characterized and purified an extracellular chitosanase (chg47) from c. globosum that is antigenic to humans. the study reports the production of monoclonal antibodies to the protein. three capture elisas were developed for chg47 for detection of spores and spore and mycelial fragments in dust samples using different mono- and po ...201425292122
psychrophilins e-h and versicotide c, cyclic peptides from the marine-derived fungus aspergillus versicolor zln-60.four new cyclic peptides, psychrophilins e-h (1-4), possessing a rare amide linkage between the carboxylic acid in anthranilic acid (ata) and the nitrogen from an indole moiety, along with a new ata-containing hexapeptide, versicotide c (5), were obtained from the culture of the marine-derived fungus aspergillus versicolor zln-60. the structures, including absolute configurations, were elucidated by a combination of hresims, nmr, x-ray crystallography, tddft ecd calculations, and marfey's method ...201425246036
aspergillines a-e, highly oxygenated hexacyclic indole-tetrahydrofuran-tetramic acid derivatives from aspergillus versicolor.aspergillines a-e (1-5) are highly oxygenated cyclopiazonic acid (cpa)-derived alkaloids bearing a rigid and sterically congested hexacyclic indole-tetrahydrofuran-tetramate scaffold, isolated from the endophytic fungus aspergillus vesicolor. apergillines a-c represent a new subclass of cpa-derived alkaloids, and aspergillines b and e possess a butanoic acid methyl ester moiety. the structures, including absolute configuration, were elucidated by interpretation of the nmr, x-ray crystallographic ...201425226561
biodegradation and utilization of 4-n-nonylphenol by aspergillus versicolor as a sole carbon and energy source.4-n-nonylphenol (4-n-np) is an environmental pollutant with endocrine-disrupting activities that is formed during the degradation of nonylphenol polyethoxylates, which are widely used as surfactants. utilization of 4-n-np by the filamentous fungus aspergillus versicolor as the sole carbon and energy source was investigated. by means of gas chromatography-mass spectrometry, we showed that in the absence of any carbon source other than 4-n-np in the medium, a. versicolor completely removed the xen ...201425222932
aspergilloma caused by aspergillus versicolor. 201425222316
total synthesis of aspeverin via an iodine(iii)-mediated oxidative cyclization.the first total synthesis of aspeverin, a prenylated indole alkaloid isolated from aspergillus versicolor in 2013, is described. key steps utilized to assemble the core structure of the target include a highly diastereoselective diels-alder reaction, a curtius rearrangement, and a unique strategy for installation of the geminal dimethyl group. a novel iodine(iii)-initiated cyclization was then used to install the bicyclic urethane linkage distinctive to the natural product.201425171639
prenylated indole diketopiperazines from the marine-derived fungus aspergillus versicolor.seven new prenylated indole diketopiperazines, versicamides a-g (1-7) and a novel chemical derivative from 7, versicamide h (8), along with three known analogic diketopiperazines (9-11) were obtained from the marine-derived fungus aspergillus versicolor hdn08-60. their structures were determined by spectroscopic techniques, including 2d nmr, ecd calculations, and single-crystal x-ray diffraction analysis, together with the assistance of further chemical conversions. the cytotoxicities of 1-8 wer ...201425089636
activation of dormant secondary metabolite production by introducing neomycin resistance into the deep-sea fungus, aspergillus versicolor zby-3.a new ultrasound-mediated approach has been developed to introduce neomycin-resistance to activate silent pathways for secondary metabolite production in a bio-inactive, deep-sea fungus, aspergillus versicolor zby-3. upon treatment of the zby-3 spores with a high concentration of neomycin by proper ultrasound irradiation, a total of 30 mutants were obtained by single colony isolation. the acquired resistance of the mutants to neomycin was confirmed by a resistance test. in contrast to the zby-3 ...201425076061
aspergillus flavus contamination in two portuguese wastewater treatment plants.filamentous fungi from genus aspergillus were previously detected in wastewater treatment plants (wwtp) as being aspergillus flavus (a. flavus), an important toxigenic fungus producing aflatoxins. this study aimed to determine occupational exposure adverse effects due to fungal contamination produced by a. flavus complex in two portuguese wwtp using conventional and molecular methodologies. air samples from two wwtp were collected at 1 m height through impaction method. surface samples were coll ...201425072712
susceptibility of green and conventional building materials to microbial growth.green building materials are becoming more popular. however, little is known about their ability to support or limit microbial growth. the growth of fungi was evaluated on five building materials. two green, two conventional building materials and wood as a positive control were selected. the materials were inoculated with aspergillus versicolor, cladosporium cladosporioides and penicillium brevicompactum, in the absence and presence of house dust. microbial growth was assessed at four different ...201524975616
[trichophyton rubrum onychomycosis with secondary aspergillus versicolor infection in a 12-year-old girl: successful topical therapy with terbinafine-urea ointment].a 12-year-old with a functional circulatory disturbance had toe nail onychomycosis caused by trichophyton rubrum. there were no other underlying diseases.201424817253
metabolites from aspergillus versicolor, an endolichenic fungus from the lichen lobaria retigera.three new anthraquinone derivatives (1-3) and one new artifact (4) were isolated, along with six known anthraquinone derivatives (5-10) and three xanthones (11-13), from a culture of an endolichenic fungus, aspergillus versicolor, that was isolated from the lichen lobaria retigera. the structures of these substances were determined on the basis of 1d and 2d (cosy, hmqc, and hmbc) nmr and ms analyses. the substances 1-4 were also tested for their cytotoxic activity.201424815583
concentration of bioaerosols in composting plants using different quantification methods.bioaerosols (organic dusts) containing viable and non-viable microorganisms and their metabolic products can lead to adverse health effects in exposed workers. standard quantification methods of airborne microorganisms are mainly based on cultivation, which often underestimates the microbial burden. the aim of the study was to determine the microbial load in german composting plants with different, mainly cultivation-independent, methods. second purpose was to evaluate which working areas are as ...201424759376
nucleoside derivatives from the marine-derived fungus aspergillus versicolor.four nucleoside derivatives (1-4) were isolated from the fungus aspergillus versicolor derived from the gorgonian dichotella gemmacea collected in the south china sea. their structures were elucidated by comprehensive spectroscopic method of nmr and ms analysis. all isolated metabolites were evaluated for their cytotoxicity, antibacterial activity and lethality towards brine shrimp artemia salina. compounds 1/2 exhibited selective antibacterial activity against staphylococcus epidermidis with an ...201424670197
allergic sensitization in canadian chronic rhinosinusitis patients.chronic rhinosinusitis (crs) is a societal burden and cause of morbidity in canada; however, the prevalence of allergic sensitization in canadian crs patients has remained poorly characterized.201424666655
enhanced production of cellulose degrading cmcase by newly isolated strain of aspergillus versicolor.production of cmcase from newly isolated strain of aspergillus versicolor was studied by optimizing different physico-chemical parameters. cellulolytic potential of the isolate was confirmed by the simple agar plate method and then subjected to variation of a single parameter at a time in cmc containing medium. maximum enzyme production was obtained at 30°c in 120 h when medium was supplemented with 0.5 gm% carboxymethyl cellulose, 0.2 gm% tween 80, 0.075 gm% peptone, 0.050 gm% cacl2, 1.5 gm% na ...201424607178
endotoxin, ergosterol, fungal dna and allergens in dust from schools in johor bahru, malaysia- associations with asthma and respiratory infections in pupils.there are few studies on associations between respiratory health and allergens, fungal and bacterial compounds in schools in tropical countries. the aim was to study associations between respiratory symptoms in pupils and ethnicity, chemical microbial markers, allergens and fungal dna in settled dust in schools in malaysia. totally 462 pupils (96%) from 8 randomly selected secondary schools in johor bahru, malaysia, participated. dust was vacuumed from 32 classrooms and analysed for levels of di ...201424523884
studies on the biosynthesis of chetomin: enantiospecific synthesis of a putative, late-stage biosynthetic intermediate.the enantiospecific synthesis of desthiochetomin, a putative biosynthetic intermediate of the epidithiodioxopiperazine natural product chetomin, is described. a diastereoselective n-alkylation was employed to form the key c3-n1' bond of the heterodimeric indoline core, followed by peptide coupling and dioxopiperazine cyclization with the requisite n-methyl amino acids. a related sarcosine-derived dioxopiperazine was prepared in the same manner. the first proposed biosynthesis of chetomin is also ...201324489414
three new sterigmatocystin analogues from marine-derived fungus aspergillus versicolor mf359.during the systematic screening of active compounds from marine-derived fungi, the extract of a strain of aspergillus versicolor mf359 isolated from a marine sponge of hymeniacidon perleve was identified for detailed chemical investigation. three new secondary metabolites, named hemiacetal sterigmatocystin (1), acyl-hemiacetal sterigmatocystin (2), and 5-methoxydihydrosterigmatocystin (3), together with a known compound, aversin (4), were characterized. 1 represents a first structure of sterigma ...201424458562
cytotoxic polyphenols from a sponge-associated fungus aspergillus versicolor hmp-48.a new dibenzo[1,4]dioxin 1, and two new prenylated diphenyl ethers, 2 and 3, together with six known compounds, 4-9, were isolated from a sponge-associated fungus aspergillus versicolor hmp-f48 by bioactivity-guided fractionation. their structures were elucidated by 1d- and 2d-nmr, and ms analyses. the compounds showed potent cell growth inhibitory activities against hl-60 cell line.201424443433
fermentation of millet to produce kamu a nigerian starch-cake food.microorganisms present in the unfermented grains of millet at the initial stage of steeping and after sieving at the initial stage of souring for the preparation of kamu were moulds (aspergillus versicolor, penicillim nigricans and rhizopus stolonifer), bacteria (pediococcus pentosaceus and lactobaclllus plantarum) and a yeast (saccharomyces cerevisiae). only the bacteria and yeast persisted to the end of the steeping period. these, together with another yeast, candida krusel, brought about the ...199124424932
ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.in rooms with moisture damage, the indoor air can be enriched with microorganisms causing a variety of symptoms. due to the highly diverse composition of bioaerosols and the multiple effects on humans, an assessment of the health risk is not sufficiently possible. the aim of this study was to characterize the features of innate immunity using blood from subjects exposed to moisture damage compared to control subjects living in houses without visible moisture damage. we investigated the expressio ...201324340055
evaluation of biotechnological potentials of some industrial fungi in economical lipid accumulation and biofuel production as a field of use.considering the vast number of scientific reports on various potential uses of fungi, there was an attempt to select the best lipid producer of some fungi at optimized conditions (aspergillus versicolor, rhizopus oryzae, rhizopus arrhizus, tramates versicolor). the aim was to offer new fields of use to the industries already culturing and using such materials. aspergillus versicolor mycelia were found to be accumulating the highest amount of lipids. experiments to improve lipid accumulation and ...201424320234
molecular and microscopic analysis of the gut contents of abundant rove beetle species (coleoptera, staphylinidae) in the boreal balsam fir forest of quebec, canada.experimental research on beetle responses to removal of logging residues following clearcut harvesting in the boreal balsam fir forest of quebec revealed several abundant rove beetle (staphylinidae) species potentially important for long-term monitoring. to understand the trophic affiliations of these species in forest ecosystems, it was necessary to analyze their gut contents. we used microscopic and molecular (dna) methods to identify the gut contents of the following rove beetles: atheta caps ...201324294095
occupational exposure to aflatoxin b1 in swine production and possible contamination sources.although the adverse health consequences of ingestion of food contaminated with aflatoxin b1 (afb1) are known, relatively few studies are available on the adverse effects of exposure in occupational settings. taking this into consideration, our study was developed aiming to elucidate the possible effects of occupational exposure to afb1 in portuguese swine production facilities using a specific biomarker to assess exposure to afb1. in total, 28 workers participated in this study, providing blood ...201324156697
biochemical and immunological analysis of mould skin prick test solution: current status of standardization.sensitization prevalence to moulds reached from less than 10% in the general population to more than 25% in atopic and/or asthmatic subjects. to diagnose ige-mediated mould sensitization, skin prick test (spt) and specific ige (sige) measurement are recommended. however, concordance of spt and sige results is often less than 50% and standardization of the extracts is required to achieve reliable test results.201324152161
[the effect of low radiation doses on the growth of aspergillus versicolor and paecilomyces lilacinus].peculiarities of growth of the strains of two species of microscopic fungi aspergillus versicolor and paecilomyces lilacinus were analyzed under exposure to chronic irradiation. it was shown that the rate of radial growth increased nonuniformly in the range of doses from 0 to 250 mgy but with maximums at a certain dose of radiation. it was shown that the absorbed dose of radiation to 2 gy did not influence the survival of investigated strains in comparison with the control ones without irradiati ...201324006782
solid-state fermentation of jatropha seed cake for optimization of lipase, protease and detoxification of anti-nutrients in jatropha seed cake using aspergillus versicolor cjs-98.this study focused on the solid-state fermentation of jatropha seed cake (jsc), a byproduct generated after biodiesel production. presence of anti-nutritional compounds and toxins restricts its application in livestock feed. the disposal of the jsc is a major environmental problem in the future, due to the generation of huge quantity of jsc after biodiesel extraction. hence the jsc was assessed for its suitability as substrate for production and optimization of lipase and protease from aspergill ...201423958640
diorcinols b-e, new prenylated diphenyl ethers from the marine-derived fungus aspergillus versicolor zln-60.four new prenylated diphenyl ethers (1-4), diorcinols b-e, together with one known diorcinol (5), were obtained from the marine-derived fungus aspergillus versicolor. their structures were established on the basis of chemical and spectroscopic data. the absolute configurations of compounds 1 and 2 were determined by mosher's ester and specific rotation analysis. their cytotoxicities were evaluated using a-549, hela, bel-7402 and k562 cell lines. compound 3 exhibited moderate cytotoxicities again ...201323677033
a new immunoassay to quantify fungal antigens from the indoor mould aspergillus versicolor.aspergillus versicolor is among the most commonly found moulds in moisture-damaged buildings and can be associated with adverse health effects in humans. this paper reports the development, validation and application of an enzyme immunoassay to quantify a. versicolor antigens. a sandwich elisa was developed using polyclonal antibodies that recognize a broad range of a. versicolor proteins present in fungal spores and in mycelia fragments. to validate the new method, a. versicolor antigens were q ...201323674292
aspeverin, a new alkaloid from an algicolous strain of aspergillus versicolor.a novel carbamate- and cyano-containing alkaloid, aspeverin (1), was isolated from the culture of an algicolous aspergillus versicolor strain (dl-29). the structure and absolute configuration were unambiguously identified by nmr, ir, ecd, and mass spectrometric methods as well as quantum chemical calculations. 1 exhibited potent bioactivities against some marine-derived organisms.201323650962
furry pet allergens, fungal dna and microbial volatile organic compounds (mvocs) in the commercial aircraft cabin environment.there has been concern about the cabin environment in commercial aircraft. we measured cat, dog and horse allergens and fungal dna in cabin dust and microbial volatile organic compounds (mvocs) in cabin air. samples were collected from two european airline companies, one with cabins having textile seats (tsc) and the other with cabins having leather seats (lsc), 9 airplanes from each company. dust was vacuumed from seats and floors in the flight deck and different parts of the cabin. cat (fel d1 ...201323644832
Refresh
firstprev
1234
nextlast
Displaying items 1 - 100 of 372