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variability in secondary structure of 18s ribosomal rna as topological marker for identification of paramecium species.besides cytological and molecular applications, paramecium is being used in water quality assessment and for determination of saprobic levels. an unambiguous identification of these unicellular eukaryotes is not only essential, but its ecological diversity must also be explored in the local environment. 18srrna genes of all the strains of paramecium species isolated from waste water were amplified, cloned and sequenced. phylogenetic comparison of the nucleotide sequences of these strains with 23 ...201425043709
stomatogenic function of the micronucleus in paramecium jenningsi.the stomatogenic function of the micronucleus in paramecium jenningsi is documented in the present report. amicronucleate cell lines were generated by removal of the micronuclei with a microinjection needle. such cell lines exhibited a temporary state of growth depression marked by the development of abnormal oral apparatuses, and they gradually recovered to nearnormal. during sexual reproduction, the amicronucleates failed to develop an oral apparatus but resorbed the pre-existing oral apparatu ...198823195465
revised systematics of holospora-like bacteria and characterization of "candidatus gortzia infectiva", a novel macronuclear symbiont of paramecium jenningsi.the genus holospora (rickettsiales) includes highly infectious nuclear symbionts of the ciliate paramecium with unique morphology and life cycle. to date, nine species have been described, but a molecular characterization is lacking for most of them. in this study, we have characterized a novel holospora-like bacterium (hlb) living in the macronuclei of a paramecium jenningsi population. this bacterium was morphologically and ultrastructurally investigated in detail, and its life cycle and infec ...201322940732
[correlations between salinity-persistence of ciliate species and their constitutive heat shock protein of 70 kda contents].our own studies of alterations in the level of constitutive heat shock protein of 70 kda family (hsp70) in freshwater (paramecium jenningsi), meta-freshwater (tetrahymena pyriformis) and curyhaline (p. nephridiatum) ciliates acclimated to salt-water and fresh-water medium were reviewed. it has been shown that the level of constitutive hsp70 content correlates with the salinity-resistance of ciliate species: in p. jenning i it was lower in freshwater, than in marine water, in euryhaline p. nephri ...201021427984
[alterations of the level of 70 kda family heat shock protein in the ciliate tetrahymena pyriformis in the process of the cells adaptation to the medium salinity changes].alterations of hsp70 level were studied in the cells of freshwater ciliate tetrahymena pyriformis after medium salinity changes. it is shown that ciliates, acclimated to fresh water (0 per thousand) and to salt water of 2 and 10 per thousand have similar constitutive levels of hsp70 in their cells. neither pronounced induction of hsp70, was not decrease of its level, revealed in ciliates after salinity stresses. these data differ from the results obtained while studying euryhaline ciliate parame ...200818771177
relationships of new sibling species of paramecium jenningsi based on sequences of the histone h4 gene fragment.paramecium jenningsi diller & earl, 1958 belongs to the "aurelia" subgroup of the genus, together with paramecium caudatum, paramecium multimicronucleatum, paramecium schewiakoffi and species of the paramecium aurelia complex. the original assumption that the morphospecies p. jenningsi was a single genetic species was questioned because a comparison of genome analyses suggested the possibility that this morphospecies contained two sibling species. to refine understanding of relationships between ...200717481867
[heat shock proteins of freshwater protists and their involvement in adaptation to changes in the environmental salinity].changes in the level of heat shock proteins (hsp) in cells of freshwater protists, amoebae amoeba proteus and ciliates paramecium jenningsi, in response to changes in the environmental salinity were investigated. changes in salinity levels were considered as a stress factor. the immunoblotting method revealed a polypeptide antigen cross-reacting with antibodies against bovine hsp70 in total protein extracts of both intact cells and cells subjected to salinity stress. the same polypeptide antigen ...200616893060
comparison of the evolutionary distances among syngens and sibling species of paramecium.the morphospecies of the genus paramecium have several mating type groups, so-called syngens, composed of cells of complementary mating types. the paramecium aurelia complex is composed of 15 sibling species assigned to the species from the syngen. to increase our understanding of the evolutionary relationships among syngen and sibling species of the genus paramecium, we investigated the gene sequences of cytosol-type hsp70 from 7 syngens of paramecium caudatum and 15 sibling species of p. aurel ...200616293424
random amplified polymorphic dna fingerprinting as a marker for paramecium jenningsi strains.the aim of the present study is to establish a common rapd marker for p. jenningsi using a series of ro primers and to investigate if strains originating from distant and isolated localities (japan, china, india, saudi arabia) have isolated gene pools and represent distinct species. an analysis of dendrograms constructed on the basis of rapd-pcr fingerprints with four primers (ro 460-04, 460-06, 460-07, and 460-10) from the first part of this project (skotarczak et al. 2004), assigns the strains ...200415521659
phylogenetic relationships of paramecium jenningsi strains (classical analysis and rapd studies).the present studies with application of classical strain crosses and rapd-pcr analyses showed the existence of different genetic species (syngens) within paramecium jenningsi. so far the existence of only one syngen has been accepted. it was found that strains from saudi arabia, india, and china compose one genetic species (syngen 1) and six strains from japan compose second genetic species (syngen 2).200314686652
the african strain of paramecium jenningsi. cytological and karyological investigations. 19807215600
observations on the behaviour of paramecium jenningsi under the influence of a sublethal dose of x-rays. 19705433414
[periods of nuclear dna synthesis in the cell cycle of paramecium jenningsi (diller and earl, 1958)]. 19744283088
genetic studies of paramecium jenningsi strains (diller, earl, 1958). 19751204897
method for the simultaneous establishment of many axenic cultures of paramecium.a method is described for the simultaneous treatment of 42 (or more) stocks of paramecium, and their adaptation to growth in axenic culture. samples of dense cultures of these ciliates growing with enterobacter aerogenes are rendered bacteria-free by migration through 2 sets of tubes containing adaptation medium (peters' salts solution, stigmaterol, vitamins, and autoclaved e. aerogenes). the 2nd set of tubes contains adaptation medium plus antibiotics. bacteria-free samples containing approxima ...1978660565
cytological and karyological studies of paramecium jenningsi. 1978648677
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