Publications

exploring the in situ accessibility of small subunit ribosomal rna of members of the domains bacteria and eukarya to oligonucleotide probes.	the principle that the small subunit ribosomal rna (ssu rrna) is generally accessible to oligonucleotide probes designed to have high thermodynamic affinity was tested with stenotrophomonas maltophilia, rhodobacter sphaeroides, bacillus subtilis, and saccharomyces cerevisiae. fluorescein-labeled probes, designed to have δg(overall)°=-14±1 and to avoid the potential of nucleobase-specific quenching, were used to target 20 randomly selected sites in each organism. a site was considered accessible ...	2012	22264799
multilocus sequence typing for clonality analysis of antimicrobial-resistant stenotrophomonas maltophilia strains.		2012	22259772
nagz-dependent and nagz-independent mechanisms for the β-lactamases expression in stenotrophomonas maltophilia.	the β-n-acetylglucosaminidase (nagz), encoded by nagz gene, is a critical enzyme for the basal-level ampc derepression (ampc expression in absence of β-lactam challenge) in ampd and dacb mutants of pseudomonas aeruginosa. three mutants with a phenotype of basal-level l1 and l2 β-lactamase derepression in stenotrophomonas maltophilia have been reported, including kjδdi (ampd(i) mutant), kjδmrca (mrca mutant), and kjδdiδmrca (ampd(i) and mrca double mutant). in this study, nagz of s. maltophilia w ...	2012	22252801
the role of nutrients in the biodegradation of 2,4,6-trinitrotoluene in liquid and soil.	the widely used explosive 2,4,6-trinitrotoluene (tnt) has residues that are potentially explosive, toxic, and mutagenic. tnt and other explosives can be degraded by microorganisms; however, biostimulation is needed for process efficiency. to investigate the effectiveness of using biostimulation to degrade tnt, we added varying concentrations of a nutrient amendment consisting of inorganic salts, plant extracts, and molasses to soil and liquid media. for the inoculum we used a consortium of bacte ...	2012	22245864
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.	abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...	2011	22243438
endowashers: an overlooked risk for possible post-endoscopic infections.	background: prevention of post-endoscopy infections is an important objective to assure patient safety. endowashers, or high throughput irrigation water pumps, are a frequently used device on endoscopes. recommendations published by professional bodies and regulatory health agencies cover not only adequate reprocessing of fiber-endoscopes but also state accepted methods of regular microbial sampling. although major instruments like endoscopes are covered by these recommendations, other devices u ...	2011	22242094
gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities.	dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ...	2012	22234511
stenotrophomonas maltophilia: an emerging global opportunistic pathogen.	summary: stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. the increasing incidence of nosocomial and community-acquired s. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. s. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. infections of s. maltophilia c ...	2012	22232370
evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders.	investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ...	2011	22205979
trends in the susceptibility of clinically important resistant bacteria to tigecycline: results from the tigecycline in-vitro surveillance in taiwan (tist), 2006-2010.	the tigecycline in-vitro surveillance in taiwan (tist), a nationwide, prospective surveillance during 2006 to 2010, collected a total of 7,793 clinical isolates, including methicillin-resistant staphylococcus aureus (mrsa) (n=1,834), penicillin-resistant streptococcus pneumoniae (prsp) (n=423), vancomycin-resistant enterococci (vre) (n=219), extended-spectrum β-lactamase (esbl)-producing escherichia coli (n=1,141), esbl-producing klebsiella pneumoniae (n=1,330), acinetobacter baumannii (n=1,645) ...	2011	22203598
biodiversity characterization of cellulolytic bacteria present on native chaco soil by comparison of ribosomal rna genes.	sequence analysis of the 16s ribosomal rna gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which actinobacteria, proteobacteria and acidobacteria accounted for more than 76% of all sequences. in both enriched samples, members of proteobacteria were the most frequently represented. the majority of ...	2011	22202170
multidrug-resistant gram-negative bacterial infections after liver transplantation - spectrum and risk factors.	objectives: gram-negative bacilli infections, especially multidrug-resistant gram-negative bacilli infections, are the leading cause of high mortality after liver transplantation. this study sought to investigate the type of infection, infection rate, pathogenic spectrum, antibiotic-resistance profile, risk factors, and epidemiology of multidrug-resistant gram-negative bacterial infection. methods: a retrospective cohort study was conducted and data of 217 liver transplant patients receiving cad ...	2011	22198738
Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs?	ABSTRACT: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortia, refining our previous observations of aerobic degradation. ...	2011	22196374
reducing the development of antibiotic resistance in critical care units.	bacteria becoming resistant to an increasing number of antibiotic classes are a major problem at hospitals including critical care units worldwide. awareness of this problem and the need to prevent the development of antibiotic resistance are very important, especially since very few new antibiotics will become available in the near future. this article gives an overview of the mechanisms of antibacterial resistance and actual resistance data worldwide of the most prevalent gram positive (mrsa, ...	2011	22188438
volatiles of two growth-inhibiting rhizobacteria commonly enroll atwrky18 function.	interactions with the (a)biotic environment play key roles for plant's fitness and vitality. in addition to direct surface-to-surface contact, volatile chemicals also affect other organisms. volatiles of serratia plymuthica and stenotrophomonas maltophilia significantly inhibited growth and induced h(2) o(2) production in arabidopsis in dual-culture. within one day, transcriptional changes could be observed by promoter-gus assays using a stress inducible, w-box containing 4xgst1 construct. expr ...	2011	22188129
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.	the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...	2011	22182219
calcite biomineralization by bacterial isolates from the recently discovered pristine karstic herrenberg cave.	karstic caves represent one of the most important subterranean carbon storages on earth and provide windows into the subsurface. the recent discovery of the herrenberg cave, germany, gave us the opportunity to investigate the diversity and potential role of bacteria in carbonate mineral formation. calcite was the only mineral observed by raman spectroscopy to precipitate as stalactites from seepage water. bacterial cells were found on the surface and interior of stalactites by confocal laser sca ...	2011	22179248
removal of high concentrations of nh(3) by a combined photoreactor and biotrickling filter system.	average emission levels as high as 800 ppm(v) nh(3) have often been found during the anaerobic fermentation process. at these levels, nh(3) is regarded as an environmental toxic compound. high concentrations of nh(3) gas are difficult to treat in a single treatment process, suggesting that, in terms of economic cost and treatment performance, a coupled system may be a feasible technological alternative. in the coupled tio(2) photocatalytic-biological treatment system evaluated here, the optimal ...	2011	22175871
effects of clinical breakpoint changes in clsi guidelines 2010/2011 and eucast guidelines 2011 on antibiotic susceptibility test reporting of gram-negative bacilli.	objectives: the aim of this study was to analyse the effects of clinical breakpoint changes in clsi 2010 and 2011 guidelines and eucast 2011 guidelines on antibiotic susceptibility testing (ast) reports. methods: in total, 3713 non-duplicate clinical isolates of enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia and acinetobacter baumannii were analysed. inhibition zone diameters were determined for β-lactams, carbapenems, fluoroquinolones, aminoglycosides and trimethoprim/ ...	2011	22167240
[the first experience of application of pcr techniques in real-time mode to diagnose bacteriemia during postoperational period in cardiosurgery patients].	the results comparison is made between pcr polymerase chain reaction techniques in real-time mode and cultural techniques of analysis of blood samples in patients with suspicion of bacteriemia during early postoperational period. from september to december 2009 43 blood samples from 37 adult patients aged 59+9.2 years were analyzed on suspicion of bacteriemia. the blood samples for pcr analysis (septifast technique roche diagnostics, switzerland) were taken parallel to the inoculation. among 43 ...	2011	22164420
polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.).	common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ...	2011	22159885
a multicenter surveillance of antimicrobial resistance on stenotrophomonas maltophilia in taiwan.	background: stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in debilitated hosts. clinical management of s. maltophilia is challenging due to its intrinsic resistance to a variety of antibiotics. this study investigated the trend and prevalence of antimicrobial resistance in s. maltophilia from a nationwide surveillance study in taiwan. methods: s. maltophilia isolates were collected biennially between 1998 and 2008 as part of the taiwan surveillance of antimicrob ...	2011	22154599
prognosis of acute respiratory distress syndrome (ards) in neutropenic cancer patients.	up today, no study has been specifically designed to identify determinants of death neutropenic cancer patients presenting ards. the aim of this study was to identify early predictive factors of 28-day mortality in these patients. factors associated with 28-day mortality during icu stay were also described.seventy consecutive cancer patients with ards and neutropenia were prospectively analysed over a 6-year period.mortality at 28-day was 63%. factors independently associated with good prognosis ...	2011	22135281
Bacterial cis-2-unsaturated fatty acids found in the cystic fibrosis airway modulate virulence and persistence of Pseudomonas aeruginosa.	There is an increasing appreciation of the polymicrobial nature of many bacterial infections such as those associated with cystic fibrosis (CF) and of the potentially important role for interspecies interactions in influencing both bacterial virulence and response to therapy. Patients with CF are often co-infected with Pseudomonas aeruginosa and other pathogens including Burkholderia cenocepacia and Stenotrophomonas maltophilia. These latter bacteria produce signal molecules of the diffusible si ...	2011	22134647
Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential.	Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequenci ...	2011	22110692
[Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus].	The pine wood nematode, Bursaphlenchus xylophilus, morphologically similar to B. mucronatus, is the pathogen of pine wilt disease. This study was focused on the endophytic bacteria present in these nematodes.	2011	22097772
detection of smqnr quinolone protection protein genes and its prevalence in clinical isolates of stenotrophomonas maltophilia in china.	to detect the novel variants of the smqnr family and analyze the prevalence of smqnr genes in clinical isolates of s. maltophilia in china. we collected 442 clinical isolates of s. maltophilia from 9 hospitals of four provinces in china. antimicrobial susceptibility test was performed for these isolates against six commonly used antibiotics (and combinations). the amplified sequences of smqnr genes using pcr were aligned with the known smqnr genes in genbank and smqnr database. the resistance ra ...	2011	22096133
antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria).	the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ...	2011	22095134
stenotrophomonas interspecies differentiation and identification by gyrb sequence analysis.	stenotrophomonas species are found commonly in environmental and clinical samples; stenotrophomonas maltophilia is an important opportunistic pathogen of humans. traditional phenotyping protocols, as well as genotyping by 16s rrna gene sequence analysis, do not reliably distinguish the species of stenotrophomonas. sequence analyses of two targeted pcr-amplified regions of the gyrb gene, which encodes the β-subunit of dna gyrase, enabled resolution and identification of these species. most type s ...	2011	22092789
cloning and overexpression of ketopantoic acid reductase gene from stenotrophomonas maltophilia and its application to stereospecific production of d: -pantoic acid.	ketopantoic acid (kpa) reductase catalyzes the stereospecific reduction of ketopantoic acid to d: -pantoic acid. based on the n-terminal amino acid sequence of kpa reductase from stenotrophomonas maltophilia 845, the kpa reductase gene was cloned from s. maltophilia nbrc14161 and sequenced. this gene contains an open reading frame of 777 bp encoding 258 amino acid residues, and the deduced amino acid sequence showed high similarity to the sdr superfamily proteins. an expression vector, petsmkpr, ...	2011	22083276
adhering ability of stenotrophomonas maltophilia is dependent on growth conditions.	the growth conditions are known to influence the bacterial adhesion to different kinds of surfaces. in the present study the adhering ability of stenotrophomonas maltophilia, on growth in nutrient rich media (tryptic soy broth (tsb)) and minimal media (luria bertani (lb)) was checked by viable cell count and spectrophotometric method. tsb grown s. maltophilia showed higher adhesion compared to bacteria grown in lb broth, to both biotic and abiotic surfaces. however, when bacteria were grown in l ...	2011	22073545
stenotrophomonas maltophilia in the respiratory tract of medical intensive care unit patients.	the purpose of this study was to investigate characteristics of critically ill patients with stenotrophomonas maltophilia (s. maltophilia) isolated from the respiratory tract, to identify risk factors for s. maltophilia-pneumonia and intensive care unit (icu) mortality and to analyze antibiotic susceptibility of s. maltophilia. this was a retrospective analysis of 64 medical icu patients with s. maltophilia in the respiratory tract. thirty-six patients fulfilled the criteria for diagnosis of pne ...	2011	22057419
Tsunami lung.	We encountered three cases of lung disorders caused by drowning in the recent large tsunami that struck following the Great East Japan Earthquake. All three were females, and two of them were old elderly. All segments of both lungs were involved in all the three patients, necessitating ICU admission and endotracheal intubation and mechanical ventilation. All three died within 3 weeks. In at least two cases, misswallowing of oil was suspected from the features noted at the time of the detection. ...	2011	22057370
characterization of phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants.	this study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. results showed that bacterial treatment followed by wetland plants (phragmites cummunis) resulted 94.5% and 96.0% reduction in bod and cod values, respectively. the pcr-rflp analysis showed the presence of stenotrophomonas, enterobacter, pantoea, acinetobacter an ...	2012	22047662
dna profiling of stenotrophomonas maltophilia by pcr targeted to its species-specific repetitive palindromic sequences.	the aim of this study was to develop a simple protocol for a pcr-based fingerprinting of stenotrophomonas maltophilia (smreppcr) that utilizes primers complementary to repetitive extragenic palindromic elements (reps) of this micro-organism.	2012	22044359
health care-associated pneumonia (hcap): empiric antibiotics targeting methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa predict optimal outcome.	inappropriate initial antimicrobial therapy (iiat) has been associated with decreased survival in patients with health care-associated pneumonia (hcap). we performed a study to determine whether empiric hcap antibiotic regimens targeting methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa are associated with greater appropriate therapy. a retrospective cohort study of culture-positive hcap over 6 years (january 2003-december 2008) was performed at barnes-jewish hospital ...	2011	22033455
Diversity and assessment of potential risk factors of Gram-negative isolates associated with French cheeses.	The goal of this study was to identify at the species level a large collection of Gram-negative dairy bacteria isolated from milks or semi-hard and soft, smear-ripened cheeses (cheese core or surface samples) from different regions of France. The isolates were then assessed for two risk factors, antibiotic resistance and volatile and non-volatile biogenic amine production in vitro. In total, 173 Gram-negative isolates were identified by rrs and/or rpoB gene sequencing. A large biodiversity was o ...	2012	22029922
microbial biofilms on the sandstone monuments of the angkor wat complex, cambodia.	discoloring biofilms from cambodian temples angkor wat, preah khan, and the bayon and west prasat in angkor thom contained a microbial community dominated by coccoid cyanobacteria. molecular analysis identified chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases. in only two of the six sites sampled were filamentous cyanobacteria, microcoleus, leptolyngbya, and scytonema, found; the first two detected by sequenc ...	2011	22006074
characterization of copper-resistant rhizosphere bacteria from avena sativa and plantago lanceolata for copper bioreduction and biosorption.	copper is a toxic heavy metal widely used to microbial control especially in agriculture. consequently, high concentrations of copper residues remain in soils selecting copper-resistant organisms. in vineyards, copper is routinely used for fungi control. this work was undertaken to study copper resistance by rhizosphere microorganisms from two plants (avena sativa l. and plantago lanceolata l.) common in vineyard soils. eleven rhizosphere microorganisms were isolated, and four displayed high res ...	2011	22002857
In vitro activity of S-(3,4-dichlorobenzyl)isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex.	The aim of this study was to establish the antimicrobial activities of S-(3,4-dichlorobenzyl)isothiourea hydrochloride (A22) and a series of structurally related compounds against multidrug-resistant (MDR) bacteria. The minimum inhibitory concentrations (MICs) of 21 compounds were determined against 18 strains of pathogenic bacteria in addition to Pseudomonas aeruginosa (n=19) and Burkholderia cepacia complex (BCC) (n=20) isolated from the sputa of cystic fibrosis patients. Selected compounds we ...	2012	21993484
biodegradation potential of oily sludge by pure and mixed bacterial cultures.	the biodegradation capacity of aliphatic and aromatic hydrocarbons of petrochemical oily sludge in liquid medium by a bacterial consortium and five pure bacterial cultures was analyzed. three bacteria isolated from petrochemical oily sludge, identified as stenotrophomonas acidaminiphila, bacillus megaterium and bacillus cibi, and two bacteria isolated from a soil contaminated by petrochemical waste, identified as pseudomonas aeruginosa and bacillus cereus demonstrated efficiency in oily sludge d ...	2011	21993328
extracellular serine proteases from stenotrophomonas maltophilia: screening, isolation and heterologous expression in e. coli.	a large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. encapsulation of cells in alginate beads induced protease production. stenotrophomonas maltophilia emerged as best performer under washing conditions. for identification of wash-active proteases, four extracellular serine proteases called stmpr1, stmpr2, stmpr3 and stmpr4 were clon ...	2012	21983234
A case of Stenotrophomonas maltophilia keratitis effectively treated with moxifloxacin.	A 70-year-old man with a long history of diabetes mellitus presented to our hospital (Department of Ophthalmology, Sahm Yook Medical Center, Seoul, Korea) complaining of severe ocular pain and visual disturbance in his left eye that had started three days prior to admission. A round 3.7 × 5.0 mm dense central stromal infiltrate with an overlying epithelial defect was noted on slit-lamp examination. Following corneal scrapings and culture, topical 0.5% moxifloxacin and 0.5% tobramycin were admini ...	2011	21976944
molecular cloning of a copper-dependent laccase from the dye-decolorizing strain stenotrophomonas maltophilia aap56.	to clone the gene encoding the enzyme with laccase activity expressed by stenotrophomonas maltophilia aap56 and to construct an insertional mutation in that gene to determine its effect on dye decolourization and laccase activity in this strain.	2011	21973274
the disinfecting potential of contact lens soutions used by sultan qaboos university students.	this study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in oman.	2011	21969898
functional characterization of an nadph dependent 2-alkyl-3-ketoalkanoic acid reductase involved in olefin biosynthesis in stenotrophomonas maltophilia.	oled is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in stenotrophomonas maltophilia. the gene coding for oled clusters with three other genes, oleabc, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. in this study, a series of substrates varying in chain length and stereochemistry were synthesized and used to elucidate the functional role and substrate specificity of oled. we demonstrated ...	2011	21958090
Identification and characterization of a chitinase of Stenotrophomonas maltophilia, a bacterium that is antagonistic towards fungal phytopathogens.	A rhizosphere strain of Stenotrophomonas maltophilia strain MUJ that is strongly antagonistic towards fungal phytopathogens secretes to the culture medium a single form of active chitinolytic enzyme belonging to family 18 of glycosyl hydrolases. The chitinase was purified by a two-stage procedure embracing fractionation with ammonium sulfate and affinity chromatography. The molecular mass of the purified enzyme determined by SDS-PAGE was approximately 52kDa. The enzyme demonstrated highest activ ...	2011	21945415
[Community acquired Stenotrophomonas maltophilia soft-tissue infection in a previously healthy patient.]		2011	21944651
Enhancement of antibiotic susceptibility of Stenotrophomonas maltophilia using a polyclonal antibody developed against an ABC multidrug efflux pump.	Stenotrophomonas maltophilia is an emerging nosocomial pathogen capable of causing healthcare-associated infections, including pneumonia and bacteremia. Intrinsic resistance in S. maltophilia is exhibited towards many broad-spectrum antibiotics, and treatment recommendations are controversial. One of the major causes of antimicrobial resistance is attributed to a robust array of efflux pumps that extrude drug compounds from the cell. Using checkerboard and growth kinetic assays, we evaluated the ...	2011	21942332
nonfermenting gram-negative bacilli infections in a tertiary care hospital in kolar, karnataka.	aim: nonfermenting gram-negative bacilli (nfgnb), which are saprophytic in nature, have emerged as important healthcare-associated pathogens. they exhibit resistance not only to beta lactam and the other groups of antibiotics, but also to carbapenems. this study was undertaken to identify the nonfermenters isolated from various clinical samples, to assess their clinical significance, to know the type of healthcare-associated infections they caused, and to know their anti-microbial sensitivity pa ...	2009	21938252
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.	Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).	2011	21936667
contribution of resistance-nodulation-division efflux pump operon smeu1-v-w-u2-x to multidrug resistance of stenotrophomonas maltophilia.	kj09c, a multidrug-resistant mutant of stenotrophomonas maltophilia kj, was generated by in vitro selection with chloramphenicol. the multidrug-resistant phenotype of kj09c was attributed to overexpression of a resistance nodulation division (rnd)-type efflux system encoded by an operon consisting of five genes: smeu1, smev, smew, smeu2, and smex. proteins encoded by smev, smew, and smex were similar to the membrane fusion protein, rnd transporter, and outer membrane protein, respectively, of kn ...	2011	21930878
Modulation of FAD-dependent monooxygenase activity from aromatic compounds-degrading Stenotrophomonas maltophilia strain KB2.	The purpose of this study was purification and characterization of phenol monooxygenase from Stenotrophomonas maltophilia strain KB2, enzyme that catabolises phenol and its derivatives through the initial hydroxylation to catechols. The enzyme requires NADH and FAD as a cofactors for activity, catalyses hydroxylation of a wide range of monocyclic phenols, aromatic acids and dihydroxylated derivatives of benzene except for catechol. High activity of this monooxygenase was observed in cell extrac ...	2011	21927719
The Ax21 protein is a cell-cell signal that regulates virulence in the nosocomial pathogen Stenotrophomonas maltophilia.	Stenotrophomonas maltophilia encodes proteins related to the Rax proteins of Xanthomonas oryzae, which are required for the synthesis and secretion of the Ax21 protein. Here we show that Ax21 acts as a cell-cell signal to regulate a diverse range of functions, including virulence, in this nosocomial pathogen.	2011	21908663
how could rapid bacterial identification improve the management of septic patients?		2011	21905779
Minimal Inhibitory Concentration of Ceftazidime and Co-trimoxazole for Stenotrophomonas Maltophilia using E-test.	Stenotrophomonas maltophilia, previously named as Pseudomonas or Xanthomonas maltophilia, is an important nosocomial pathogen	2011	21887057
antibacterial effects of theaflavin and synergy with epicatechin against clinical isolates of acinetobacter baumannii and stenotrophomonas maltophilia.	stenotrophomonas maltophilia and acinetobacter baumannii are recognised as important nosocomial pathogens; however, due to their intrinsic resistance to multiple antibiotics, treatment options are limited. polyphenols from black tea have been shown to possess antibacterial action. in this study, the antibacterial effects of various concentrations of theaflavin as well as combinations of theaflavin and epicatechin were determined using the disk diffusion assay. the results showed strong antibacte ...	2011	21885260
characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of herculaneum in italy.	microbiological characterization of frescos in four different locations (collegio degli augustali, casa del colonnato tuscanico, casa dello scheletro and casa del gran portale) of excavation sites of herculaneum was carried out. the use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. the microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming an ...	2011	21882662
Characteristics of microbial aerosols released from chicken and swine feces.	Bioaerosols generated during livestock and poultry production are significant occupational hazards. This study investigates the characteristics of bioaerosols released from animal feces. Fresh feces from pigs and chickens were obtained and tested in a controlled-environment facility. Airborne viable (culturable) bacteria and fungi were sampled hourly for 48 hr. The predominant species were identified via polymerase chain reaction analysis. The number of bacterial colonies released from chicken f ...	2011	21874960
Reducing Sugar-Producing Bacteria from Guts of Tenebrio Molitor Linnaeus (Yellow mealworm) for Lignocellulosic Waste Minimization.	The guts of Tenebrio Molitor Linnaeus (yellow mealworm) were used as inocula to isolate reducing sugar-producing bacteria during bioconversion of lignocellulose to reducing sugars in this study. Three carbon sources, i.e., carboxymethyl cellulose (CMC), filter paper (FP), and lignocellulosic waste (LIG), were specifically selected; and two types of culturing media (M1 and M2) were used. After 6 months of sequential cultivation, lignocellulose (i.e., polysaccharides) degradation of enrichments M1 ...	2011	21869568
[distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse].	to investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control.	2011	21866707
distribution of nosocomial organisms and their resistance patterns in the intensive care unit of the university hospital of the west indies, kingston, jamaica.	to determine the distribution and antibiotic susceptibility patterns of nosocomial pathogens in the intensive care unit (icu) at the university hospital of the west indies (uhwi).	2009	21866600
effect of surfactants on pah biodegradation by a bacterial consortium and on the dynamics of the bacterial community during the process.	the aim of this work was to evaluate the effect of a non-biodegradable (tergitol np-10) and a biodegradable (tween-80) surfactant on growth, degradation rate and microbial dynamics of a polycyclic aromatic hydrocarbon (pahs) degrading consortium (c2pl05) from a petroleum polluted soil, applying cultivable and non cultivable techniques. growth and degradation rate were significantly lower with tergitol np-10 than that with tween-80. toxicity did not show any significant reduction with tergitol np ...	2011	21862321
Frequency of oral mucositis and microbiological analysis in children with acute lymphoblastic leukemia treated with 0.12% chlorhexidine gluconate.	In view of the morbidity potential of oral complications in patients with leukemia, this study evaluated the clinical and microbiological alterations that occur in the oral mucosa of children with acute lymphoblastic leukemia (ALL) undergoing antineoplastic chemotherapy and prophylactic administration of 0.12% chlorhexidine gluconate. The sample consisted of 17 children aged 2 to 12 years that underwent clinical examination of the oral mucosa for the detection of oral lesions. In addition, biolo ...	2011	21861031
hessian fly-associated bacteria: transmission, essentiality, and composition.	plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ...	2011	21858016
screening for novel quorum-sensing inhibitors to interfere with the formation of pseudomonas aeruginosa biofilm.	the objective of this study was to screen for novel quorum-sensing inhibitors (qsis) from traditional chinese medicines (tcms) that inhibit bacterial biofilm formation. six of 46 active components found in tcms were identified as putative qsis based on molecular docking studies. of these, three compounds inhibited biofilm formation by pseudomonas aeruginosa and stenotrophomonas maltophilia at a concentration of 200 µm. a fourth compound (emodin) significantly inhibited biofilm formation at 20 µm ...	2011	21852522
Contemporary unconventional clinical use of co-trimoxazole.	Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ...	2012	21851485
chronic stenotrophomonas maltophilia infection and exacerbation outcomes in cystic fibrosis.	chronic stenotrophomonas maltophilia infection is a risk factor for pulmonary exacerbation in cystic fibrosis (cf) but its impact on subsequent clinical outcomes is unknown. the aim of this study was to determine the effect of chronic s. maltophilia infection and associated antimicrobial therapy on the recovery of forced expiratory lung volume in 1s (fev(1)) following pulmonary exacerbation.	2011	21849265
antibacterial and anti-biofilm effects of cathelicidin peptides against pathogens isolated from cystic fibrosis patients.	six different cathelicidin-derived peptides were compared to tobramycin for antibacterial and anti-biofilm effects against s. aureus, p. aeruginosa, and s. maltophilia strains isolated from cystic fibrosis patients. overall, smap-29, bmap-28, and bmap-27 showed relevant antibacterial activity (mic(50) 4-8μg/ml), and in some cases higher than tobramycin. in contrast, indolicidin, ll-37, and bac7(1-35) showed no significant antimicrobial activity (mic(50)>32μg/ml). killing kinetics experiments sho ...	2011	21849157
arsenic-resistant bacteria solubilized arsenic in the growth media and increased growth of arsenic hyperaccumulator pteris vittata l.	the role of arsenic-resistant bacteria (arb) in arsenic solubilization from growth media and growth enhancement of arsenic-hyperaccumulator pteris vittata l. was examined. seven arb (tolerant to 10mm arsenate) were isolated from the p. vittata rhizosphere and identified by 16s rrna sequencing as pseudomonas sp., comamonas sp. and stenotrophomonas sp. during 7-d hydroponic experiments, these bacteria effectively solubilized arsenic from the growth media spiked with insoluble feaso(4) and alaso(4) ...	2011	21840210
Low Frequency of Endemic Patient-to-Patient Transmission of Antibiotic-Resistant Gram-Negative Bacilli in a Pediatric Intensive Care Unit.	We sought to determine the frequency of horizontal transmission of antibiotic-resistant gram-negative bacilli (ARGNB) in a pediatric intensive care unit during a nonoutbreak period. Among 5,300 admissions over 39 consecutive months, 13 ARGNB clusters involving 35 children were identified by pulsed-filed gel electrophoresis analysis, which suggests that person-to-person transmission was uncommon.	2011	21828974
ciprofloxacin in critically ill children.	data regarding the use of fluoroquinolones in critically ill children are scarce. we present our experience regarding the use of ciprofloxacin in this specific patient population. we prospectively identified all paediatric patients who received ciprofloxacin treatment in the intensive care unit of the tertiary care p. & a. kyriakou children's hospital during a three year period (2005 to 2008). eighteen paediatric patients (mean age 23 months, 12 females) who received intravenous ciprofloxacin we ...	2011	21823382
identification of stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure.	aims:ôçé aim of the study is to identify accurately stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. methods and results:ôçé recovery of sten.-ámaltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin b (via) selective agar medium enabled distinction of various morphotype colonies. a set of soil and clinical isolates was tested for species identification using different methods. 16s rdna analyses showed the dark green with a ...	2011	21819497
use of the diversilab repetitive sequence-based polymerase chain reaction system for genotyping stenotrophomonas maltophilia isolates in different wards of one hospital.		2011	21802174
emerging gram-negative infections in burn wounds.	gram-negative infection remains a major contributor to morbidity, mortality, and cost of care. in the absence of comparative multinational epidemiological studies specific to burn patients, we sought to review literature trends in emerging gram-negative burn wound infections within the past 60 years. mapping trends in these organisms, although in a minority compared with the six "eskape" pathogens currently being targeted by the infectious diseases society of north america, would identify pathog ...	2011	21792068
biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon.	three strains of gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp. based on physio-biochemistry analysis and 16s rrna gene sequence analysis. removal efficiencies of 2 mg/l geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp., respectively, while removal efficiencies of ...	2011	21790055
xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.	the xenopus laevis oocyte has been the workhorse for the investigation of ion transport proteins. these large cells have spawned a multitude of novel techniques that are unfathomable in mammalian cells, yet the fickleness of the oocyte has driven many researchers to use other membrane protein expression systems. here, we show that some colonies of xenopus laevis are infected with three multi-drug-resistant bacteria: pseudomonas fluorescens, pseudomonas putida, and stenotrophomonas maltophilia. o ...	2011	21788613
an unusual cause of continuous ambulatory peritoneal dialysis-associated outpatient peritonitis: stenotrophomonas maltophilia.		2011	21782283
stevens-johnson syndrome in a juvenile systemic lupus erythematosus patient.	stevens-johnson syndrome (sjs) is a severe and rare immune-mediated cutaneous reaction usually induced by drugs or infections. few case reports have demonstrated sjs associated with adult systemic lupus erythematosus (sle), and rarely in juvenile sle (jsle) patients. however, to the best of our knowledge the prevalence of this life-threatening cutaneous disease in the pediatric lupus population has not been studied. therefore, from january 1983 to december 2010, 5508 patients were followed-up at ...	2011	21768172
stenotrophomonas maltophilia in cystic fibrosis: improved detection by the use of selective agar and evaluation of antimicrobial resistance.	background: we implemented a selective medium for improved detection of stenotrophomonas maltophilia in sputum samples from cf patients. we also performed antimicrobial susceptibility testing with eight antibiotics. methods: a total of 623 consecutive sputum samples from 165 cf patients in a german cf center were cultured onto conventional media and onto steno medium agar (sma). all isolates confirmed as s. maltophilia by biochemical and molecular methods were subjected to antimicrobial suscepti ...	2011	21763218
c-s targeted biodegradation of dibenzothiophene by stenotrophomonas sp. nisoc-04.	crude oil-contaminated soil samples were gathered across khuzestan oilfields (national iranian south oil company, nisoc) consequently experienced a screening procedure for isolating c-s targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a c-s targeted manner in aqueous phases and medium mostly consisting of separately biphasic water-gasoline. the ...	2011	21750993
infection in immunocompromised hosts and the organ transplant recipient -essentials.		2011	21748845
testosterone-mineralizing culture enriched from swine manure: characterization of degradation pathways and microbial community composition.	environmental releases and fate of steroid sex hormones from livestock and wastewater treatment plants are of increasing regulatory concern. despite the detection of these hormones in manures, biosolids, and the environment, little attention has been paid to characterization of fecal bacteria capable of hormone degradation. the enrichments of (swine) manure-borne bacteria capable of aerobic testosterone degradation were prepared and the testosterone mineralization pathway was elucidated. six dna ...	2011	21740029
an mlsa-based online scheme for the rapid identification of stenotrophomonas isolates.	an online scheme to assign stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (mlsa), which is based on the dna sequencing of selected fragments of the housekeeping genes atp synthase alpha subunit (atpa), the recombination repair protein (reca), the rna polymerase alpha subunit (rpoa) and the excision repair beta subunit (uvrb). this mlsa-based scheme was validated using eight of the 10 stenotrophomonas species that have been previously described. t ...	2011	21739025
the binding of triclosan to smet, the repressor of the multidrug efflux pump smedef, induces antibiotic resistance in stenotrophomonas maltophilia.	the wide utilization of biocides poses a concern on the impact of these compounds on natural bacterial populations. furthermore, it has been demonstrated that biocides can select, at least in laboratory experiments, antibiotic resistant bacteria. this situation has raised concerns, not just on scientists and clinicians, but also on regulatory agencies, which are demanding studies on the impact that the utilization of biocides may have on the development on resistance and consequently on the trea ...	2011	21738470
acceleration of nonylphenol and 4-tert-octylphenol degradation in sediment by phragmites australis and associated rhizosphere bacteria.	we investigated biodegradation of technical nonylphenol (tnp) in phragmites australis rhizosphere sediment by conducting degradation experiments using sediments spiked with tnp. accelerated tnp removal was observed in p. australis rhizosphere sediment, whereas tnp persisted in unvegetated sediment without plants and in autoclaved sediment with sterile plants, suggesting that the accelerated tnp removal resulted largely from tnp biodegradation by rhizosphere bacteria. three bacterial strains, ste ...	2011	21736332
phenotypic and genotypic characterization of stenotrophomonas maltophilia isolates from patients with cystic fibrosis: genome diversity, biofilm formation, and virulence.	abstract:	2011	21729271
inactivation of mrca gene derepresses the basal-level expression of l1 and l2 {beta}-lactamases in stenotrophomonas maltophilia.	objectives to characterize the relationship between inactivation of the mrca gene and +¦-lactamase expression and +¦-lactams resistance in stenotrophomonas maltophilia kj and to investigate the involvement of ampr, ampn-ampg, ampd(i) and crebc in this. methods the mrca deletion mutant kj+ömrca was constructed to investigate the role of this putative penicillin-binding protein 1a (pbp1a) in +¦-lactamase expression and +¦-lactam resistance. the +öampr, +öampng, +öampdi and +öcrebc alleles were int ...	2011	21719470
diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze).	the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ...	2011	21715960
nile red detection of bacterial hydrocarbons and ketones in a high-throughput format.	a method for use in high-throughput screening of bacteria for the production of long-chain hydrocarbons and ketones by monitoring fluorescent light emission in the presence of nile red is described. nile red has previously been used to screen for polyhydroxybutyrate (phb) and fatty acid esters, but this is the first report of screening for recombinant bacteria making hydrocarbons or ketones. the microtiter plate assay was evaluated using wild-type and recombinant strains of shewanella oneidensis ...	2011	21712420
resolving the phylogenetic and taxonomic relationship of xanthomonas and stenotrophomonas strains using complete rpob gene sequence.		2011	21686310
genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere.	the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ...	2011	21686169
mucosal surveillance cultures in predicting gram-negative late-onset sepsis in neonatal intensive care units.	this study aimed to examine the spectrum and time course of gut and nasopharyngeal colonization with gram-negative micro-organisms, and to define the value of surveillance cultures in predicting late-onset sepsis in neonates admitted to neonatal intensive care units. nasopharyngeal and rectal swabs were collected on admission and twice weekly thereafter in 278 neonates admitted within the first 72-áh of life with risk factors of early-onset sepsis. sterile body fluid cultures were obtained on ad ...	2011	21684633
phylogenetic analysis of culturable marine bacteria in sediments from the south korean yellow sea.	biogeochemical and microbiological characterization of marine sediments taken from the yellow sea of south korea was carried out. one hundred and thirty six bacterial strains were isolated, characterized and phylogenetic relationship was evaluated. the gene sequences of 16s rdna regions were examined to study the phylogenetic analysis of bacterial community in the marine sediments. among 136 isolates, 5 bacterial isolates were identified as novel members, remaining 131 isolates were fall into 5 ...	2011	21675219
antimicrobial susceptibilities of commonly encountered bacterial isolates to fosfomycin determined by agar dilution and disk diffusion methods.	we studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. species studied included 3 common species of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia; methicillin-susceptible and -resistant staphylococcus aureus; and vancomycin-susceptible and resistant enterococcus faecalis and e. faecium. mics and ...	2011	21670185
diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis.	microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ...	2011	21660545
culture-dependent and -independent molecular analysis of the bacterial community within uranium ore.	the bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16s rrna genes. the major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. together with near neutral ph, moderate organic carbon content, elevated u and other heavy metals (v, ni, mn, cu, etc.), the ore showed high microbial count ...	2011	21656801
fatal hemorrhagic pneumonia caused by stenotrophomanas maltophilia in a patient with non-hodgkin lymphoma.	stenotrophomonas maltophilia is increasingly emerging as a multiresistant pathogen in the hospital environment. in immunosuppressed patients, this bacterium may cause severe infections associated with sepsis and multiple organ dysfunction. we report on a 57-year-old woman treated with intensive chemotherapy for non-hodgkin lymphoma who developed severe neutropenia, hemorrhagic pneumonia, and acute respiratory failure, which led to her death within 36 h of onset of pneumonia. postmortem examinati ...	2011	21656194
a comparative study of biodegradation of vinyl acetate by environmental strains.	four gram-negative strains, e3_2001, ec1_2004, ec3_3502 and ec2_3502, previously isolated from soil samples, were subjected to comparative studies in order to select the best vinyl acetate degrader for waste gas treatment. comparison of biochemical and physiological tests as well as the results of fatty acids analyses were comparable with the results of 16s rrna gene sequence analyses. the isolated strains were identified as pseudomonas putida ec3_2001, pseudomonas putida ec1_2004, achromobacter ...	2011	21654921
stenotrophomonas maltophilia causing early onset neonatal sepsis.	stenotrophomonas maltophilia, a multi drug resistant non fermenting gram negative bacillus is an increasingly common nosocomial pathogen, especially in intensive care units. comparatively few cases of infection have been reported in neonatal population. we present two cases of early onset neonatal sepsis due to s.maltophilia and a brief review of documented isolation in neonates.	2011	21654005