Publications

development of a murine infection model with leishmania killicki, responsible for cutaneous leishmaniosis in algeria: application in pharmacology.	in algeria, leishmania infantum, leishmania major, and leishmania killicki (leishmania tropica) are responsible for cutaneous leishmaniosis. we established a murine model of l. killicki infection to investigate its infective capacity, some immunophysiopathological aspects, and its suitability for pharmacological purposes. following the injection of l. major or l. killicki metacyclic promastigotes in the ear dermis of balb/c mice, the course of infection was followed. the infection with l. killic ...	2016	26949705
comparison of leishmania killicki (syn. l. tropica) and leishmania tropica population structure in maghreb by microsatellite typing.	leishmania (l.) killicki (syn. l. tropica), which causes cutaneous leishmaniasis in maghreb, was recently described in this region and identified as a subpopulation of l. tropica. the present genetic analysis was conducted to explore the spatio-temporal distribution of l. killicki (syn. l. tropica) and its transmission dynamics. to better understand the evolution of this parasite, its population structure was then compared with that of l. tropica populations from morocco. in total 198 samples in ...	2015	26645812
natural infection of algerian hedgehog, atelerix algirus (lereboullet 1842) with leishmania parasites in tunisia.	in tunisia, leishmania parasites are responsible of visceral leishmaniasis, caused by leishmania infantum species while three cutaneous disease forms are documented: chronic cutaneous leishmaniasis due to leishmania killicki, sporadic cutaneous form (scl) caused by l. infantum and the predominant zoonotic cutaneous leishmanaisis (zcl) due to leishmania major. zcl reservoirs are rodents of the psammomys and meriones genera, while for scl the dog is supposed to be a reservoir. ctenodactylus gundii ...	2015	26086952
evolutionary history of leishmania killicki (synonymous leishmania tropica) and taxonomic implications.	the taxonomic status of leishmania (l.) killicki, a parasite that causes chronic cutaneous leishmaniasis, is not well defined yet. indeed, some researchers suggested that this taxon could be included in the l. tropica complex, whereas others considered it as a distinct phylogenetic complex. to try to solve this taxonomic issue we carried out a detailed study on the evolutionary history of l. killicki relative to l. tropica.	2015	25889939
remote sensing, land cover changes, and vector-borne diseases: use of high spatial resolution satellite imagery to map the risk of occurrence of cutaneous leishmaniasis in ghardaïa, algeria.	ghardaïa, central algeria, experienced a major outbreak of cutaneous leishmaniasis (cl) in 2005. two leishmania species occur in this region: leishmania major (mon-25) and leishmania killicki (mon-301). the two species are transmitted respectively by the sandflies phlebotomus papatasi and phlebotomus sergenti and probably involve rodent reservoirs with different ecologies, suggesting distinct epidemiological patterns and distribution areas. the aims of this study were to establish risk maps for ...	2014	25305006
phlebotomus (paraphlebotomus) riouxi: a synonym of phlebotomus chabaudi without any proven vectorial role in tunisia and algeria.	phlebotomus (paraphlebotomus) riouxi depaquit, léger & killick-kendrick (diptera: psychodidae) was described as a typological species based on a few morphological characters distinguishing it from phlebotomus (paraphlebotomus) chabaudi croset, abonnenc & rioux. the naming of p. riouxi coincided with its incrimination as a rural vector of leishmania tropica wright (junior synonym: leishmania killicki rioux, lanotte & pratlong) in tataouine governorate, an arid region of southern tunisia. the curr ...	2014	25171607
heterogeneity of molecular resistance patterns in antimony-resistant field isolates of leishmania species from the western mediterranean area.	antimonials remain the first-line treatment for the various manifestations of leishmaniasis in most areas where the disease is endemic, and increasing cases of therapeutic failure associated with parasite resistance have been reported. in this study, we assessed the molecular status of 47 clinical isolates of leishmania causing visceral and cutaneous leishmaniasis from algeria, tunisia, and southern france. in total, we examined 14 genes that have been shown to exhibit significant variations in ...	2014	24913173
cutaneous leishmaniasis caused by leishmania killicki, algeria.		2014	24565368
identification of tunisian leishmania spp. by pcr amplification of cysteine proteinase b (cpb) genes and phylogenetic analysis.	discrimination of the old world leishmania parasites is important for diagnosis and epidemiological studies of leishmaniasis. we have developed pcr assays that allow the discrimination between leishmania major, leishmania tropica and leishmania infantum tunisian species. the identification was performed by a simple pcr targeting cysteine protease b (cpb) gene copies. these pcr can be a routine molecular biology tools for discrimination of leishmania spp. from different geographical origins and d ...	2013	23228525
geographical distribution updating of tunisian leishmaniasis foci: about the isoenzymatic analysis of 694 strains.	over a period of ten years, a series of 694 leishmania strains from tunisian leishmaniasis foci were isolated and identified by isoenzymatic analysis. strains were obtained from human cutaneous and visceral leishmaniasis in immunocompetent subjects, visceral leishmaniasis in imunocompromised individuals and from dogs with visceral leishmaniasis. two classically dermotropic species, leishmania (l.) major and leishmania killicki were found. l. major with the single zymodeme mon-25 was the most iso ...	2012	22940099
twenty-four new human cases of cutaneous leishmaniasis due to leishmania killicki in metlaoui, southwestern tunisia: probable role of phlebotomus sergenti in the transmission.	metlaoui district in the south-west of tunisia is a classical focus of cutaneous leishmaniasis (cl) due to leishmania major. since 2005, a single case of cl due to l. killicki has been reported. we report twenty four human cases due to this parasite, affecting men and women from 2 to 70 years old. leishmania killicki have been typed using molecular techniques: polymerase chain reaction (pcr) followed by restriction fragment length polymorphism (rflp) and gene sequencing. four strains from patien ...	2012	22306359
first detection of leishmania killicki (kinetoplastida, trypanosomatidae) in ctenodactylus gundi (rodentia, ctenodactylidae), a possible reservoir of human cutaneous leishmaniasis in tunisia.	abstract:	2011	21834996
phlebotomus sergenti (parrot, 1917) identified as leishmania killicki host in gharda+»a, south algeria.	since 2005, an outbreak of human cutaneous leishmaniasis (cl) in gharda+»a, south algeria, was studied and one output of these investigations was the identification of two leishmania species, leishmania major and leishmania killicki, as the cl causative agents. in the present study, we were curious to focus on sand fly fauna present in this area and detection of leishmania-positive sand fly females. sand flies (3717) were collected during two seasons using sticky papers and cdc light traps in ur ...	2011	21382502
development of a polymerase chain reaction-restriction fragment length polymorphism assay for leishmania major/leishmania killicki/leishmania infantum discrimination from clinical samples, application in a tunisian focus.	topoisomerase ii gene of leishmania genus was used to develop a molecular tool for detection and species differentiation of leishmania from clinical samples. identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases bstu1 and taq1. despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.	2010	20846587
leishmania killicki imported from tunisian desert.		2009	19891892
geographical distribution and epidemiological features of old world cutaneous leishmaniasis foci, based on the isoenzyme analysis of 1048 strains.	a series of 1048 leishmania strains from old world cutaneous leishmaniasis foci, isolated between 1981 and 2005, were studied by isoenzyme analysis. the strains were obtained from humans, rodents, dogs and sandflies from 33 countries. the four typically dermotropic species, leishmania major, l. tropica, l. aethiopica and l. killicki, were found. the viscerotropic species l. donovani and l. infantum, which can occasionally be responsible for cutaneous leishmaniasis, are not considered in this pap ...	2009	19624480
[molecular and morphological characterisation of two closely related species belonging to the subgenus phlebotomus chabaudi croset, abonnenc & rioux, 1970 et p. riouxi depaquit, killick-kendrick & léger, 1998 (diptera: psychodidae)].	in 1998, the description of phlebotomus riouxi emphasised the difficulty to differentiate the female from the closely related p. chabaudi, a suspected vector of leishmania killicki in several foci in tunisia. in order to be able to distinguish the females of these two species, we started a molecular study based on 37 algerian and tunisian specimens. the alignment of the sequences of the cytochrome b of the mitochondrial dna and their analysis using neighbor-joining, maximum likelihood and maximu ...	2008	19202763
[cutaneous leishmaniasis caused by leishmania infantum mon-24 in tunisia: extension of the focus to the center of the country].	three clinico-epidemiological forms of cutaneous leishmaniasis (cl) were described in tunisia: the zoonotic cl (zcl) epidemic which occurred in the centre of the country caused by leishmania major mon-25, the chronic cl (ccl) in the south-east of the country caused by leishmania killicki mon-8 and the sporadic cl in the north (scl) caused by leishmania infantum mon-24. the latter form, described in 1991, prevails in northern tunisia with approximately thirty cases per year. its vector, unknown f ...	2008	18432004
anthroponotic cutaneous leishmaniasis in tunisia: presence of leishmania killicki outside its original focus of tataouine.	the first three documented cases of anthroponotic cutaneous leishmaniasis due to leishmania killicki are reported from locations outside the original focus of tataouine in southeast tunisia. three strains were isolated from three patients from gafsa, sidi bouzid and seliana indicating an extension of this parasite's range towards the centre and the north of tunisia.	2005	15869775
spread of leishmania killicki to central and south-west tunisia.	twenty cutaneous leishmaniasis (cl) cases were notified from december 2001 to february 2002, in a small village in the district of oueslatia (governorate of kairouan, central tunisia) which is an endemic focus of infantile visceral leishmaniasis due to leishmania (l.) infantum and that had never been concerned previously by cl. the parasite typing of two isolates obtained from two children that have never left the region has identified l. killicki. this species had only been reported previously ...	2005	15828583