Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| cloning and characterization of f3pyc gene encoding pyruvate carboxylase in aspergillus flavus strain (f3). | pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and l-malic acid. these organic acids play very important role for biological remediation of heavy metals. in this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (f3pyc) from heavy metal resistant indigenous fungal isolate aspergillus flavus (f3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a c ... | 2017 | 28710744 |
| aspergillus fischeri mediated biosynthesis of gold nanoparticles and their beneficially comparative effect on normal and cancer cell lines. | background along with the intensified use of metal nanoparticles, growing concern of their adverse outcome on human health has also expanded, indicating that this work is an integral part of nanobioscience study. objective the aim of this study was to evaluate varied effect of biosynthesized gold nanoparticles (aunps) on normal and cancerous mammalian cells. methods aunp synthesized and characterized by different characterization methods, here are produced by specifically isolated aspergillus sp ... | 2017 | 28530537 |
| structural determinants of neosartorya fischeri antifungal protein (nfap) for folding, stability and antifungal activity. | the recent global challenges to prevent and treat fungal infections strongly demand for the development of new antifungal strategies. the structurally very similar cysteine-rich antifungal proteins from ascomycetes provide a feasible basis for designing new antifungal molecules. the main structural elements responsible for folding, stability and antifungal activity are not fully understood, although this is an essential prerequisite for rational protein design. in this study, we used the neosart ... | 2017 | 28512317 |
| loop 3 of fungal endoglucanases of glycoside hydrolase family 12 modulates catalytic efficiency. | glycoside hydrolase (gh) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. however, the important roles of the loop regions of gh12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. this study examined how the loop 3 region affects the enzymatic properties of gh12 glucanases using nfeg12a from neosartorya fischeri p1 and eg (pdb 1ks4) from aspergillus niger acidophilic and thermophilic nfeg12a had the hig ... | 2017 | 28039140 |
| occurrence of azole-resistant species of aspergillus in the uk environment. | the aim of this study was to survey environmental isolates of aspergillus resistant to azoles in azole-treated and naïve areas to determine whether resistance could be related to azole treatment history. aspergillus fumigatus was sampled from the centre of a large city and from fields with known azole history. azole resistance was determined and sequencing was performed to identify strains and mutations in the cyp51a gene. azole resistance was detected in azole-treated field isolates but not in ... | 2014 | 27873687 |
| a penicillium chrysogenum-based expression system for the production of small, cysteine-rich antifungal proteins for structural and functional analyses. | small, cysteine-rich and cationic antifungal proteins (aps) from filamentous ascomycetes, such as nfap from neosartorya fischeri and paf from penicillium chrysogenum, are promising candidates for novel drug development. a prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. technologies for structure analyses, such as electronic circular dichroism (ecd) ... | 2016 | 27835989 |
| nfap2, a novel cysteine-rich anti-yeast protein from neosartorya fischeri nrrl 181: isolation and characterization. | the increasing incidence of fungal infections and damages due to drug-resistant fungi urges the development of new antifungal strategies. the cysteine-rich antifungal proteins from filamentous ascomycetes provide a feasible base for protection against molds due to their potent antifungal activity on them. in contrast to this, they show no or weak activity on yeasts, hence their applicability against this group of fungi is questionable. in the present study a 5.6 kda anti-yeast protein (nfap2) is ... | 2016 | 27637945 |
| biochemical properties and evaluation of washing performance in commercial detergent compatibility of two collagenolytic serine peptidases secreted by aspergillus fischeri and penicillium citrinum. | filamentous fungi secrete diverse peptidases with different biochemical properties, which is of considerable importance for application in various commercial sectors. in this study, we describe the isolation of two fungal species collected from the soil of decayed organic matter: aspergillus fischeri and penicillium citrinum. in a submerged bioprocess, we observed better peptidase production with the fungus p. citrinum, which reached a peak production at 168 h with 760 u/ml, in comparison with t ... | 2017 | 27552601 |
| heterologous production of an acidic thermostable lipase with broad-range ph activity from thermophilic fungus neosartorya fischeri p1. | thermophilic neosartorya fischeri p1 is an excellent lipase producer and harbors seven lipase genes. all genes were found to be functional after heterologous expression in escherichia coli. one of them, lip09, showed high-level expression in pichia pastoris with the yield of 2.0 g/l in a 3.7-l fermentor. deduced amino acid sequence of lip09 consists of a putative signal peptide (residues 1-19) and a mature polypeptide (residues 20-562). compared with other fungal counterparts, purified recombina ... | 2016 | 27503553 |
| revisiting overexpression of a heterologous β-glucosidase in trichoderma reesei: fusion expression of the neosartorya fischeri bgl3a to cbh1 enhances the overall as well as individual cellulase activities. | the filamentous fungus trichoderma reesei has the capacity to secret large amounts of cellulase and is widely used in a variety of industries. however, the t. reesei cellulase is weak in β-glucosidase activity, which results in accumulation of cellobiose inhibiting the endo- and exo-cellulases. by expressing an exogenous β-glucosidase gene, the recombinant t. reesei cellulase is expected to degrade cellulose into glucose more efficiently. | 2016 | 27400964 |
| aspergillus fumigatus-related species in clinical practice. | aspergillus fumigatus is the main etiologic agent of invasive aspergillosis (ia). other aspergillus species belonging to the section fumigati (a. fumigatus complex) may occasionally be the cause of ia. these strains are often misidentified, as they cannot be distinguished from a. fumigatus by conventional morphological analysis and sequencing methods. this lack of recognition may have important consequences as these a. fumigatus-related species often display some level of intrinsic resistance to ... | 2016 | 27242710 |
| [neosartorya fischeri (wehmer) malloch & cain]. | 2016 | 26965879 | |
| crude extracts of marine-derived and soil fungi of the genus neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines. | the crude ethyl acetate extracts of marine-derived fungi neosartorya tsunodae kufc 9213 (e1) and n. laciniosa kufc 7896 (e2), and soil fungus n. fischeri kufc 6344 (e3) were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines. | 2017 | 26941530 |
| biotransformation of petroleum asphaltenes and high molecular weight polycyclic aromatic hydrocarbons by neosartorya fischeri. | neosartorya fischeri, an aspergillaceae fungus, was evaluated in its capacity to transform high molecular weight polycyclic aromatics hydrocarbons (hmw-pahs) and the recalcitrant fraction of petroleum, the asphaltenes. n. fischeri was able to grow in these compounds as sole carbon source. coronene, benzo(g,h,i)perylene, and indeno(1,2,3-c,d)pyrene, together with the asphaltenes, were assayed for fungal biotransformation. the transformation of the asphaltenes and hmw-pahs was confirmed by reverse ... | 2016 | 26893177 |
| comparison of chemical sensitivity of fresh and long-stored heat resistant neosartorya fischeri environmental isolates using biolog phenotype microarray system. | spoilage of heat processed food and beverage by heat resistant fungi (hrf) is a major problem for food industry in many countries. neosartorya fischeri is the leading source of spoilage in thermally processed products. its resistance to heat processing and toxigenicity makes studies about neosartorya fischeri metabolism and chemical sensitivity essential. in this study chemical sensitivity of two environmental neosartorya fischeri isolates were compared. one was isolated from canned apples in 19 ... | 2016 | 26815302 |
| hydrophilins in the filamentous fungus neosartorya fischeri (aspergillus fischeri) have protective activity against several types of microbial water stress. | hydrophilins are proteins that occur in all domains of life and protect cells and organisms against drought and other stresses. they include most of the late embryogenesis abundant (lea) proteins and the heat shock protein (hsp) hsp12. here, the role of a predicted lea-like protein (leama) and two hsp12 proteins (hsp12a and hsp12b) of neosartorya fischeri was studied. this filamentous fungus forms ascospores that belong to the most stress-resistant eukaryotic cells described to date. heterologou ... | 2016 | 26487515 |
| microarray analysis of neosartorya fischeri using different carbon sources, petroleum asphaltenes and glucose-peptone. | asphaltenes are considered as the most recalcitrant petroleum fraction and represent a big problem for the recovery, separation and processing of heavy oils and bitumens. neosartorya fischeri is a saprophytic fungus that is able to grow using asphaltenes as the sole carbon source [1]. we performed transcription profiling using a custom designed microarray with the complete genome from n. fischeri nrrl 181 in order to identify genes related to the transformation of asphaltenes [1]. data analysis ... | 2015 | 26484261 |
| a novel gh7 endo-β-1,4-glucanase from neosartorya fischeri p1 with good thermostability, broad substrate specificity and potential application in the brewing industry. | an endo-β-1,4-glucanase gene, cel7a, was cloned from the thermophilic cellulase-producing fungus neosartorya fischeri p1 and expressed in pichia pastoris. the 1,410-bp full-length gene encodes a polypeptide of 469 amino acids consisting of a putative signal peptide at residues 1-20, a catalytic domain of glycoside hydrolase family 7 (gh7), a short thr/ser-rich linker and a family 1 carbohydrate-binding module (cbm 1). the purified recombinant cel7a had ph and temperature optima of ph 5.0 and 60° ... | 2015 | 26360701 |
| genome mining for sesterterpenes using bifunctional terpene synthases reveals a unified intermediate of di/sesterterpenes. | genome mining is a promising method to discover novel secondary metabolites in the postgenomic era. we applied the aspergillus oryzae heterologous expression system to functionally characterize cryptic bifunctional terpene synthase genes found in fungal genomes and identified the sesterfisherol synthase gene (nfss) from neosartorya fischeri. sesterfisherol contains a characteristic 5-6-8-5 tetracyclic ring system and is modified by cytochrome p450 monooxygenase (nfp450) to sesterfisheric acid. t ... | 2015 | 26332841 |
| biochemical characterization of three distinct polygalacturonases from neosartorya fischeri p1. | polygalacturonase is one of the most important industrial pectinases. to enrich the genetic resources and develop new enzyme candidates, three polygalacturonase genes (nfpg i-iii) of glycosyl hydrolase family 28 were cloned from neosartorya fischeri p1 and functionally expressed in pichia pastoris. the purified recombinant proteins exhibited some distinguished properties. in comparison with other counterparts, nfpg i showed the highest specific activity (40, 123 u/mg), nfpg ii had the highest te ... | 2015 | 26041232 |
| insight into the antifungal mechanism of neosartorya fischeri antifungal protein. | small, cysteine-rich, highly stable antifungal proteins secreted by filamentous ascomycetes have great potential for the development of novel antifungal strategies. however, their practical application is still limited due to their not fully clarified mode of action. the aim of this work was to provide a deep insight into the antifungal mechanism of neosartorya fischeri antifungal protein (nfap), a novel representative of this protein group. within a short exposure time to nfap, reduced cellular ... | 2015 | 25994413 |
| structural analysis of novel trehalose-based oligosaccharides from extremely stress-tolerant ascospores of neosartorya fischeri (aspergillus fischeri). | different fungi, including the genera neosartorya, byssochlamys and talaromyces, produce (asco)spores that survive pasteurization treatments and are regarded as the most stress-resistant eukaryotic cells. here, the nmr analysis of a series of trehalose-based oligosaccharides, being compatible solutes that are accumulated to high levels in ascospores of the fungus neosartorya fischeri, is presented. these oligosaccharides consist of an α,α-trehalose backbone, extended with one [α-d-glcp-(1 → 6)-α ... | 2015 | 25974853 |
| the first case of feline sinonasal aspergillosis due to aspergillus fischeri in japan. | feline upper respiratory tract infection due to aspergillus spp. is considered an emerging disease, with the number of reported cases continuing to rise. in this study, we report the first case of feline sinonasal aspergillosis caused by aspergillus fischeri in japan. the patient presented after 2 months of progressive facial deformity around the nose and nasal discharge. the isolate from this case was susceptible to itraconazole (itz), voriconazole and micafungin, but was resistant to amphoteri ... | 2015 | 25947561 |
| targeted production of secondary metabolites by coexpression of non-ribosomal peptide synthetase and prenyltransferase genes in aspergillus. | prenylated tryptophan-containing cyclic dipeptides are found in different fungi and serve as precursors for the biosynthesis of diverse biologically active secondary metabolites. they show distinct and usually higher biological and pharmacological activities than the respective non-prenylated dipeptides. successful production of such compounds were achieved by a new approach based on the coexpression of ftmps, a non-ribosomal peptide synthetase from neosartorya fischeri, with three cyclic dipept ... | 2015 | 25744649 |
| enhanced biological straw saccharification through coculturing of lignocellulose-degrading microorganisms. | lignocellulosic waste (lcw) is an abundant, low-cost, and inedible substrate for the induction of lignocellulolytic enzymes for cellulosic bioethanol production using an efficient, environmentally friendly, and economical biological approach. in this study, 30 different lignocellulose-degrading bacterial and 18 fungal isolates were quantitatively screened individually for the saccharification of four different ball-milled straw substrates: wheat, rice, sugarcane, and pea straw. rice and sugarcan ... | 2015 | 25724976 |
| sartorypyrone d: a new nadh-fumarate reductase inhibitor produced by neosartorya fischeri fo-5897. | 2015 | 25586023 | |
| erratum to: insights into the substrate specificity and synergy with mannanase of family 27 α-galactosidases from neosartorya fischeri p1. | 2015 | 25573475 | |
| avenaciolides: potential mura-targeted inhibitors against peptidoglycan biosynthesis in methicillin-resistant staphylococcus aureus (mrsa). | discovery of new antibiotics for combating methicillin-resistant staphylococcus aureus (mrsa) is of vital importance in the post-antibiotic era. here, we report four avenaciolide derivatives (1-4) isolated from neosartorya fischeri, three of which had significant antimicrobial activity against mrsa. the morphology of avenaciolide-treated cells was protoplast-like, which indicated that cell wall biosynthesis was interrupted. comparing the structures and minimum inhibitory concentrations of 1-4, t ... | 2015 | 25521652 |
| insights into the substrate specificity and synergy with mannanase of family 27 α-galactosidases from neosartorya fischeri p1. | thermophilic neosartorya fischeri p1 is an excellent carbohydrate-active enzyme (cazyme) producer. two α-galactosidases of gh (glycoside hydrolase ) family 27 with a very low sequence identity (28.7%), gal27a and gal27b, were identified in strain p1 and functionally expressed in pichia pastoris. in comparison to other characterized gh27 fungal counterparts, rgal27b has a higher temperature optimum (75 °c) and better thermostability (>50% activity at 70 °c for 15 min), and rgal27a shows stability ... | 2015 | 25487894 |
| a thermophilic β-mannanase from neosartorya fischeri p1 with broad ph stability and significant hydrolysis ability of various mannan polymers. | a new β-mannanase gene, man5p1, was cloned from the thermophilic fungus neosartorya fischeri p1, and successfully expressed in pichia pastoris. the predicted amino acid sequence of man5p1 consists of a putative 19-residue signal peptide at the n-terminus and a catalytic domain of glycoside hydrolase family 5. the purified recombinant man5p1 (rman5p1) was optimally active at ph 4.0 and 80 °c, and was acid and alkali tolerant, exhibiting >20% of the maximal activity at ph 2.0 and 9.0. rman5p1 had ... | 2015 | 25466024 |
| modeling the growth of byssochlamys fulva and neosartorya fischeri on solidified apple juice by measuring colony diameter and ergosterol content. | byssochlamys fulva and neosartorya fischeri are heat-resistant fungi which are a concern to food industries (e.g. apple juice industry) since their growth represents significant economic liabilities. although the most common method used to assess fungal growth in solid substrates is by measuring the colony's diameter, it is difficult to apply this method to food substrates. alternatively, ergosterol contents have been used to quantify fungal contamination in some types of food. the current study ... | 2015 | 25462919 |
| fungal colonization and enzyme-mediated metabolism of waste coal by neosartorya fischeri strain eccn 84. | colonization and oxidative metabolism of south african low-rank discard coal by the fungal strain eccn 84 previously isolated from a coal environment and identified as neosartorya fischeri was investigated. results show that waste coal supported fungal growth. colonization of waste coal particles by n. fischeri eccn 84 was associated with the formation of compact spherical pellets or sclerotia-like structures. dissection of the pellets from liquid cultures revealed a nucleus of "engulfed" coal w ... | 2014 | 25273148 |
| molecular characterization of a highly-active thermophilic β-glucosidase from neosartorya fischeri p1 and its application in the hydrolysis of soybean isoflavone glycosides. | isoflavone occurs abundantly in leguminous seeds in the form of glycoside and aglycone. however, isoflavone glycoside has anti-nutritional effect and only the free type is beneficial to human health. in the present study we identified a β-glucosidase from thermophilic neosartorya fischeri p1, termed nfbgl1, capable of efficiently converting isoflavone glycosides into free isoflavones. the gene, belonging to glycoside hydrolase family 3, was successfully overexpressed in pichia pastoris at high c ... | 2014 | 25188254 |
| functionality and prevalence of trehalose-based oligosaccharides as novel compatible solutes in ascospores of neosartorya fischeri (aspergillus fischeri) and other fungi. | ascospores of neosartorya, byssochlamys and talaromyces can be regarded as the most stress-resistant eukaryotic cells. they can survive exposure at temperatures as high as 85°c for 100 min or more. neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of talaromyces macrosporus which contain predominantly trehalose. these ascospores contain trehalose-based oligosaccharides (tos) that are novel compatible solutes, wh ... | 2014 | 25040129 |
| a decrease in bulk water and mannitol and accumulation of trehalose and trehalose-based oligosaccharides define a two-stage maturation process towards extreme stress resistance in ascospores of neosartorya fischeri (aspergillus fischeri). | fungal propagules survive stresses better than vegetative cells. neosartorya fischeri, an aspergillus teleomorph, forms ascospores that survive high temperatures or drying followed by heat. not much is known about maturation and development of extreme stress resistance in fungal cells. this study provides a novel two-step model for the acquisition of extreme stress resistance and entry into dormancy. ascospores of 11- and 15-day-old cultures exhibited heat resistance, physiological activity, acc ... | 2015 | 25040022 |
| survey of molds, yeast and alicyclobacillus spp. from a concentrated apple juice productive process. | bacteria and molds may spoil and/or contaminate apple juice either by direct microbial action or indirectly by the uptake of metabolites as off-flavours and toxins. some of these microorganisms and/or metabolites may remain in the food even after extensive procedures. this study aim to identify the presence of molds (including heat resistant species) and alicyclobacillus spp., during concentrated apple juice processing. molds were isolated at different steps and then identified by their macrosco ... | 2014 | 24948913 |
| a promiscuous prenyltransferase from aspergillus oryzae catalyses c-prenylations of hydroxynaphthalenes in the presence of different prenyl donors. | prenyltransferases of the dimethylallyltryptophan synthase (dmats) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. most members of this superfamily accepted as prenyl donor exclusively dimethylallyl diphosphate (dmapp). one enzyme, anapt from neosartorya fischeri, was reported recently to use both dmapp and geranyl diphosphate (gpp) as prenyl do ... | 2014 | 24430210 |
| mannitol is essential for the development of stress-resistant ascospores in neosartorya fischeri (aspergillus fischeri). | the polyol mannitol is one of the main compatible solutes in neosartorya fischeri and accumulates in conidia and ascospores. here, it is shown that biosynthesis of mannitol in n. fischeri mainly depends on mannitol 1-phosphate dehydrogenase (mpda). reporter studies and qpcr analysis demonstrated that mpda is moderately expressed in vegetative hyphae and conidiophores, while it is highly expressed during development of ascospores. deletion of mpda reduced mannitol in whole cultures as much as 85% ... | 2014 | 24412483 |
| a thermophilic α-galactosidase from neosartorya fischeri p1 with high specific activity, broad substrate specificity and significant hydrolysis ability of soymilk. | an extracellular α-galactosidase (gal27a) with high specific activity of 423umg(-1) was identified in thermophilic neosartorya fischeri p1. its coding gene (1680bp) was cloned and functionally expressed in pichia pastoris. sequence analysis indicated that deduced gal27a contains a catalytic domain of glycoside hydrolase family 27. the native and recombinant enzymes shared some similar properties, such as ph optima at 4.5, temperature optima at 60-70°c, resistance to most chemicals and saccharide ... | 2014 | 24360500 |
| production of a defensin-like antifungal protein nfap from neosartorya fischeri in pichia pastoris and its antifungal activity against filamentous fungal isolates from human infections. | neosartorya fischeri nrrl 181 isolate secretes a defensin-like antifungal protein (nfap) which has a remarkable antifungal effect against ascomycetous filamentous fungi. this protein is a promising antifungal agent of biotechnological value; however in spite of the available knowledge of the nature of its 5'-upstream transcriptional regulation elements, the bulk production of nfap has not been resolved yet. in this study we carried out its heterologous expression in the yeast pichia pastoris and ... | 2014 | 24269762 |
| genome mining for new α-amylase and glucoamylase encoding sequences and high level expression of a glucoamylase from talaromyces stipitatus for potential raw starch hydrolysis. | mining fungal genomes for glucoamylase and α-amylase encoding sequences led to the selection of 23 candidates, two of which (designated tsgam-2 and nfamy-2) were advanced to testing for cooked or raw starch hydrolysis. tsgam-2 is a 66-kda glucoamylase recombinantly produced in pichia pastoris and originally derived for talaromyces stipitatus. when harvested in a 20-l bioreactor at high cell density (od600 > 200), the secreted tsgam-2 enzyme activity from p. pastoris strain gs115 reached 800 u/ml ... | 2014 | 24046254 |
| a new approach to microbial production of gallic acid. | in a new approach to microbial gallic acid production by aspergillus fischeri mtcc 150, 40gl(-1) of tannic acid was added in two installments during the bioconversion phase of the process (25gl(-1) and 15gl(-1) at 32 and 44h respectively). the optimum parameters for the bioconversion phase were found to be temperature: 35°c, ph: slightly acidic (3.3-3.5), aeration: nil and agitation: 250 rpm. a maximum of 71.4% conversion was obtained after 71h fermentation with 83.3% product recovery. the yield ... | 2008 | 24031294 |
| geranylation of cyclic dipeptides by the dimethylallyl transferase anapt resulting in a shift of prenylation position on the indole ring. | the dimethylallyl transferase anapt from neosartorya fischeri is involved in the biosynthesis of acetylaszonalenin and catalyses the regioselective and stereospecific c3α-prenylation of (r)-benzodiazepinedione in the presence of dimethylallyl diphosphate. this enzyme also converts several tryptophan-containing cyclic dipeptides to c3α-prenylated indolines. in this study, we demonstrate the geranylation of (r)-benzodiazepinedione and five other cyclic dipeptides by anapt in the presence of gerany ... | 2013 | 24014429 |
| clinical significance and molecular characterization of nonsporulating molds isolated from the respiratory tracts of bronchopulmonary mycosis patients with special reference to basidiomycetes. | nonsporulating molds (nsms), especially basidiomycetes, have predominantly been reported as human pathogens responsible for allergic and invasive disease. their conventional identification is problematic, as many isolates remain sterile in culture. thus, inconclusive culture reports might adversely affect treatment decisions. the clinical significance of nsms in pulmonary mycoses is poorly understood. we sequenced the internal transcribed spacer (its) region and d1/d2 domain of the larger subuni ... | 2013 | 23903552 |
| cdpc2pt, a reverse prenyltransferase from neosartorya fischeri with a distinct substrate preference from known c2-prenyltransferases. | a putative prenyltransferase gene, nfia_043650, was amplified from neosartorya fischeri nrrl 181 and cloned into the expression vector pqe60. the deduced polypeptide consisting of 445 amino acids with a molecular mass of 51 kda was overproduced in escherichia coli and purified as his6-tagged protein to near homogeneity. the purified soluble protein was subsequently assayed with potential aromatic substrates in the presence of dimethylallyl diphosphate. hplc analysis of the reaction mixtures reve ... | 2013 | 23845975 |
| functional properties of a manganese-activated exo-polygalacturonase produced by a thermotolerant fungus aspergillus niveus. | a thermotolerant fungus identified as aspergillus niveus was isolated from decomposing materials and it has produced excellent levels of hydrolytic enzymes that degrade plant cell walls. a. niveus germinated faster at 40 °c, presenting protein levels almost twofold higher than at 25 °c. the crude extract of the a. niveus culture was purified by diethylaminoethyl (deae)-cellulose, followed by biogel p-100 column. polygalacturonase (pg) is a glycoprotein with 37.7 % carbohydrate, molecular mass of ... | 2013 | 23613286 |
| disparate proteome responses of pathogenic and nonpathogenic aspergilli to human serum measured by activity-based protein profiling (abpp). | aspergillus fumigatus is the primary pathogen causing the devastating pulmonary disease invasive aspergillosis in immunocompromised individuals. there is high genomic synteny between a. fumigatus and closely related rarely pathogenic neosartorya fischeri and aspergillus clavatus genomes. we applied activity-based protein profiling to compare unique or overexpressed activity-based probe-reactive proteins of all three fungi over time in minimal media growth and in response to human serum. we found ... | 2013 | 23599423 |
| effect of different fruit components, aeration and cold storage at 5°c on the germination of ascospores of neosartorya fischeri. | germination is the process by which a spore is transformed from a dormant state of metabolic activity to one of high activity. effects of different fruit components, aeration and cold storage at 5°c on the germination of ascospores of neosartorya fischeri after heat treatment were investigated by measuring rate and percentage germination and outgrowth of fungal biomass. some activated spores reverted back to dormancy after 12 and 24 h of storage at 5°c. ascospores germinated easily in acetate bu ... | 2013 | 23530777 |
| genome mining of a prenylated and immunosuppressive polyketide from pathogenic fungi. | activation of the polycyclic polyketide prenyltransferase (pcptase)-containing silent clusters in aspergillus fumigatus and neosartorya fischeri led to isolation of a new metabolite neosartoricin (3). the structure of 3 was solved by x-ray crystallography and nmr to be a prenylated anthracenone. 3 exhibits t-cell antiproliferative activity with an ic(50) of 3 μm, suggestive of a physiological role as an immunosuppressive agent. | 2013 | 23368997 |
| complexity generation in fungal peptidyl alkaloid biosynthesis: a two-enzyme pathway to the hexacyclic mdr export pump inhibitor ardeemin. | ardeemins are hexacyclic peptidyl alkaloids isolated from aspergillus fischeri as agents that block efflux of anticancer drugs by multidrug resistance (mdr) export pumps. to evaluate the biosynthetic logic and enzymatic machinery for ardeemin framework assembly, we sequenced the a. fischeri genome and identified the ardabc gene cluster. through both genetic deletions and biochemical characterizations of purified arda and ardb we show this ardab enzyme pair is sufficient to convert anthranilate ( ... | 2013 | 23330675 |
| sequencing of mitochondrial genomes of nine aspergillus and penicillium species identifies mobile introns and accessory genes as main sources of genome size variability. | the genera aspergillus and penicillium include some of the most beneficial as well as the most harmful fungal species such as the penicillin-producer penicillium chrysogenum and the human pathogen aspergillus fumigatus, respectively. their mitochondrial genomic sequences may hold vital clues into the mechanisms of their evolution, population genetics, and biology, yet only a handful of these genomes have been fully sequenced and annotated. | 2012 | 23234273 |
| investigation of the antimicrobial effect of neosartorya fischeri antifungal protein (nfap) after heterologous expression in aspergillus nidulans. | neosartorya fischeri antifungal protein (nfap) is a β-defensin-like peptide produced by the n. fischeri nrrl 181 isolate. in this study, we investigated the manifestation of the antimicrobial effect of nfap via heterologous expression of the nfap gene in an nfap-sensitive fungus, aspergillus nidulans. heterologous expression of the nfap gene was carried out in a. nidulans cs2902 using a pama1-based autonomous replicative vector construct. the effect of the produced nfap on the germination of a. ... | 2013 | 23197172 |
| identification of the verruculogen prenyltransferase ftmpt3 by a combination of chemical, bioinformatic and biochemical approaches. | previous studies showed that verruculogen is the end product of a biosynthetic gene cluster for fumitremorgin-type alkaloids in aspergillus fumigatus and neosartorya fischeri. in this study, we isolated fumitremorgin a from n. fischeri. this led to the identification of the responsible gene, ftmpt3, for o-prenylation of an aliphatic hydroxy group in verruculogen. this gene was found at a different location in the genome of n. fischeri than the identified cluster. the coding sequence of ftmpt3 wa ... | 2012 | 23109474 |
| detection and purification of non-ribosomal peptide synthetase products in neosartorya fischeri. | non-ribosomal peptide synthetases (nrpss) are important enzymes of production machinery for natural products including clinically used antibiotics, antifungal, and anticancer agents. nrps products are usually further modified by tailoring enzymes, resulting in the formation of diverse structures. we demonstrate here the production and isolation of metabolites produced by two bi-modular nrpss, i.e., acetylaszonalenin and fumitremorgin-type alkaloids in neosartorya fischeri nrrl181. | 2012 | 23065611 |
| diversity and specificity of microsatellites within aspergillus section fumigati. | microsatellites (or short tandem repeats, strs) are the genetic markers of choice for studying aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. however, the specificity of these markers must be investigated in a group of isolates from closely related species. the aim of this work was to test a microsatellite-based pcr multiplex previously designed for a. fumigatus in a set of species belonging to section fumigati, namely aspergillus fumigatia ... | 2012 | 22838495 |
| discovery and characterization of a group of fungal polycyclic polyketide prenyltransferases. | the prenyltransferase (ptase) gene vrtc was proposed to be involved in viridicatumtoxin (1) biosynthesis in penicillium aethiopicum. targeted gene deletion and reconstitution of recombinant vrtc activity in vitro established that vrtc is a geranyl transferase that catalyzes a regiospecific friedel-crafts alkylation of the naphthacenedione carboxamide intermediate 2 at carbon 6 with geranyl diphosphate. vrtc can function in the absence of divalent ions and can utilize similar naphthacenedione sub ... | 2012 | 22590971 |
| biochemical characterization of a gh53 endo-β-1,4-galactanase and a gh35 exo-β-1,4-galactanase from penicillium chrysogenum. | an endo-β-1,4-galactanase (pcgal1) and an exo-β-1,4-galactanase (pcgalx35c) were purified from the culture filtrate of penicillium chrysogenum 31b. pcgal1 and pcgalx35c cdnas encoding pcgal1 and pcgalx35c were isolated by in vitro cloning. the deduced amino acid sequences of pcgal1 and pcgalx35c are highly similar to a putative endo-β-1,4-galactanase of aspergillus terreus (70% amino acid identity) and a putative β-galactosidase of neosartorya fischeri (72%), respectively. pfam analysis revealed ... | 2013 | 22584433 |
| a heme peroxidase of the ascomyceteous lichen leptogium saturninum oxidizes high-redox potential substrates. | lichens belonging to the order peltigerales display strong activity of multi-copper oxidases (e.g. tyrosinase) as well as heme-containing peroxidases. the lichen peroxidase was purified to homogeneity from the thallus of leptogium saturninum (lsapox) by fast protein liquid chromatography and then partially characterized. the oligomeric protein occurs as both 79 kda dimeric and 42 kda monomeric forms, and displayed broad substrate specificity. in addition to an ability to oxidize classic peroxida ... | 2011 | 22056522 |
| characterization of a recombinant aryl β-glucosidase from neosartorya fischeri nrrl181. | an isolated gene from neosartorya fischeri nrrl181 encoding a β-glucosidase (bgl) was cloned, and its nucleotide sequence was determined. dna sequence analysis revealed an open reading frame of 1,467 bp, capable of encoding a polypeptide of 488 amino acid residues. the gene was over-expressed in escherichia coli, and the protein was purified using nickel-nitrilotriacetic acid chromatography. the purified recombinant bgl showed a high level of catalytic activity, with v (max) ... | 2011 | 22042231 |
| new resources for functional analysis of omics data for the genus aspergillus. | detailed and comprehensive genome annotation can be considered a prerequisite for effective analysis and interpretation of omics data. as such, gene ontology (go) annotation has become a well accepted framework for functional annotation. the genus aspergillus comprises fungal species that are important model organisms, plant and human pathogens as well as industrial workhorses. however, go annotation based on both computational predictions and extended manual curation has so far only been availa ... | 2011 | 21974739 |
| Removal of arsenic from aqueous environments by native and chemically modified biomass of Aspergillus niger and Neosartorya fischeri. | Arsenic removal from aqueous solutions by biomass of two fungal strains, Aspergillus niger and Neosartorya fischeri, was assessed. The biosorption capacity of fungal biomass was studied within the As(V) concentration range of approximately 0.2 to 5.0 mg L(-1) at two different pH values (pH 5 and 7). With increasing initial arsenic concentration, the biosorption capacity of both fungal strains increased almost linearly and achieved the sorption capacity of 0.317 and 0.124 mg g(-1) for biomass of ... | 2011 | 21970163 |
| biosorption and biovolatilization of arsenic by heat-resistant fungi (5 pp). | the aim of this work is to show the ability of several fungal species, isolated from arsenic polluted soils, to biosorb and volatilize arsenic from a liquid medium under laboratory conditions. mechanisms of biosorption and biovolatilization play an important role in the biogeochemical cycle of arsenic in the environment. the quantification of production of volatile arsenicals is discussed in this article. | 2007 | 21959538 |
| Bioactive metabolites from a marine-derived strain of the fungus Neosartorya fischeri. | Two new compounds named fischeacid (1) and fischexanthone (2), together with eight known compounds, were obtained from the culture of a marine-derived fungus Neosartorya fischeri strain 1008F(1). The structures of the new compounds were elucidated based on the spectroscopic data. Bioassays indicated that AGI-B4 (5) and 3,4-dihydroxybenzoic acid (10) showed potent inhibitory effect on the replication of tobacco mosaic virus, and AGI-B4 also possessed an inhibition of the cell proliferation of hum ... | 2011 | 21916772 |
| isolation and characterization of neosartorya fischeri antifungal protein (nfap). | a novel 6.6kda antifungal peptide (nfap) from the culture supernatant of the mold, neosartorya fischeri (anamorf: aspergillus fischerianus), and its encoding gene were isolated in this study. nfap is a small, basic and cysteine-rich protein consisting of 57 amino acid residues. it shows 37.9-50% homology to similar proteins described in literature from aspergillus clavatus, aspergillus giganteus, aspergillus niger, and penicillium chrysogenum. the in silico presumed tertiary structure of nfap, e ... | 2011 | 21741420 |
| molecular organization of the mating-type loci in the homothallic ascomycete eupenicillium crustaceum. | eupenicillium species are the teleomorphic (sexual) forms of anamorphic (asexual) members of the genus penicillium, which contains many species of industrial importance. here we describe the first molecular analysis of the mating-type (mat) locus from a homothallic (self-fertile) eupenicillium species, e. crustaceum. this ascomycete is a sexual relative of the penicillin producer penicillium chrysogenum, which while long considered asexual, was recently shown to possess the required genetic mach ... | 2011 | 21724167 |
| first evidence of mineralization of petroleum asphaltenes by a strain of neosartorya fischeri. | a fungal strain isolated from a microbial consortium growing in a natural asphalt lake is able to grow in purified asphaltenes as the only source of carbon and energy. the asphaltenes were rigorously purified in order to avoid contamination from other petroleum fractions. in addition, most of petroporphyrins were removed. the 18s rrna and β-tubulin genomic sequences, as well as some morphologic characteristics, indicate that the isolate is neosartorya fischeri. after 11 weeks of growth, the fung ... | 2011 | 21624102 |
| permanent genetic resources added to molecular ecology resources database 1 october 2009-30 november 2009. | this article documents the addition of 411 microsatellite marker loci and 15 pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: acanthopagrus schlegeli, anopheles lesteri, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus oryzae, aspergillus terreus, branchiostoma japonicum, branchiostoma belcheri, colias behrii, coryphopterus personatus, cynogolssus semilaevis, cy ... | 2010 | 21565039 |
| rapid identification of aspergillus fumigatus within the section fumigati. | abstract: | 2011 | 21510879 |
| sinonasal and sino-orbital aspergillosis in 23 cats: aetiology, clinicopathological features and treatment outcomes. | aetiology, clinicopathological findings and treatment outcomes were documented in 23 cats (1.5-13years of age) with sinonasal (sna, n=6) or sino-orbital (soa, n=17) aspergillosis. cases recruited retrospectively and prospectively were included if fungal hyphae were identified on cytological or histological examination and the fungal pathogen was identified by pcr and dna sequencing (its1 or its1-5.8s-its2 regions, rdna gene cluster). fungal culture was positive in 22/23 cases. in cases of sna, t ... | 2011 | 21388842 |
| sequential opportunistic infections in two german shepherd dogs. | two german shepherd dogs with sequential opportunistic infections are described. the first was a 2-year-old male with cryptococcal rhinitis that spread to involve the optic nerves and brain. it was successfully treated with combination therapy utilising amphotericin b administered for 2 years, but the dog developed a disseminated aspergillus deflectus infection 5 years later and was euthanased. the second case was a 4-year-old male that presented for a severe, deep-seated infection of the right ... | 2011 | 21250949 |
| the human ku autoantigen shares amino acid sequence homology with fungal, but not bacterial and viral, proteins. | context: molecular mimicry between autoantigens and microbial antigens is a possible triggering mechanism of autoimmunity. human ku is a dna-associated autoantigen targeted by autoantibodies in patients with systemic lupus erythematosus and related disorders; available data are consistent with a role of molecular mimicry in the pathogenesis of anti-ku autoimmunity, but no research exist on this topic. objective: we aimed to define the most probable microbial triggers of anti-ku autoimmunity via ... | 2011 | 20718578 |
| high-level expression of a specific beta-1,3-1,4-glucanase from the thermophilic fungus paecilomyces thermophila in pichia pastoris. | in this study, a novel beta-1,3-1,4-glucanase gene (designated as ptlic16a) from paecilomyces thermophila was cloned and sequenced. ptlic16a has an open reading frame of 945 bp, encoding 314 amino acids. the deduced amino acid sequence shares the highest identity (61%) with the putative endo-1,3(4)-beta-glucanase from neosartorya fischeri nrrl 181. ptlic16a was cloned into a vector ppic9k and was expressed successfully in pichia pastoris as active extracellular beta-1,3-1,4-glucanase. the recomb ... | 2010 | 20645085 |
| the role of a plant/fungal consortium in the degradation of bituminous hard coal. | the sporadic growth of cynodon dactylon was observed to occur directly on the surface of hard coal in dumps of the witbank coal mining area of south africa with the surface coal being broken down into a humic-like particulate material. microorganism analysis of plants and rhizosphere material from the dumps revealed the presence of arbuscular mycorrhizal fungi and the coal solubilising fungus, neosartorya fischeri. studies established to replicate the dump environment revealed increased coal deg ... | 2011 | 20602151 |
| preparation of pyrrolo[2,3-b]indoles carrying a beta-configured reverse c3-dimethylallyl moiety by using a recombinant prenyltransferase cdpc3pt. | six beta-configured reversely c3-prenylated pyrrolo[2,3-b]indoles were successfully prepared by using a recombinant prenyltransferase from neosartorya fischeri. for this purpose, the putative prenyltransferase gene nfia_074280 (termed herewith cdpc3pt) was cloned into pqe60 and overexpressed in escherichia coli. the overproduced his(6)-cdpc3pt was purified to near homogeneity and incubated with five cyclic tryptophan-containing dipeptides in the presence of dimethylallyl diphosphate (dmapp). all ... | 2010 | 20448903 |
| anthranilate-activating modules from fungal nonribosomal peptide assembly lines. | fungal natural products containing benzodiazepinone- and quinazolinone-fused ring systems can be assembled by nonribosomal peptide synthetases (nrps) using the conformationally restricted beta-amino acid anthranilate as one of the key building blocks. we validated that the first module of the acetylaszonalenin synthetase of neosartorya fischeri nrrl 181 activates anthranilate to anthranilyl-amp. with this as a starting point, we then used bioinformatic predictions about fungal adenylation domain ... | 2010 | 20225828 |
| conformational and functional transitions in class ii alpha-mannosidase from aspergillus fischeri. | the conformational transitions in an oligomeric and high molecular weight class ii alpha-mannosidase from aspergillus fischeri were examined using fluorescence and cd spectroscopy under chemical, thermal and acid denaturing conditions. the enzyme lost the activity first and then the overall folded conformation and secondary structure. the midpoint values of gdnhcl mediated changes measured by inactivation; fluorescence and negative ellipticity were 0.48 m, 1.5 m and 1.9 m, respectively. the prot ... | 2010 | 20204683 |
| reconstruction of pyrrolo[2,3-b]indoles carrying an alpha-configured reverse c3-dimethylallyl moiety by using recombinant enzymes. | nine reversely c3-prenylated pyrrolo[2,3-b]indoles were successfully prepared by using two recombinant enzymes involved in the biosynthesis of acetylaszonalenin from neosartorya fischeri. the prenyltransferase anapt catalysed the conversion of six tryptophan-containing cyclic dipeptides to reversely c3-prenylated indoline derivatives. using cyclo-l-trp-l-trp as substrate, both mono- and diprenylated indolines were obtained. two of the anapt products were acetylated at position n1 by the acetyltr ... | 2010 | 20165805 |
| [phylogenetic analysis and detection of selective pressure on the cvnh domains]. | cyanovirin-n (cv-n) is a novel protein with broad-spectrum antiviral activity. its homologs constitute a protein family known as cvnh (cyanovirin-n homology), which possess the evolutionarily conserved anti-hiv (human immunodeficiency virus) domain. in this study, more details about the patchy organism distribution of cvnh domain were explored by reconstructing gene trees. duplicated cvnh sequences were also identified in a wide range of species including aspergillus niger, neosartorya fischeri ... | 2010 | 20085891 |
| identification of two marine fungi and evaluation of their antivirus and antitumor activities. | to identify two marine fungi and evaluate the inhibitory effects of their crude extracts on tobacco mosaic virus and two tumor cell lines. | 2009 | 20030065 |
| applications of dimethylallyltryptophan synthases and other indole prenyltransferases for structural modification of natural products. | a series of putative indole prenyltransferase genes could be identified in the genome sequences of different fungal strains including aspergillus fumigatus and neosartorya fischeri. the gene products show significant sequence similarities to dimethylallyltryptophan synthases from various fungi. these genes belong to different gene clusters and are involved in the biosynthesis of secondary metabolites. ten of them were cloned and overexpressed in escherichia coli and saccharomyces cerevisiae and ... | 2009 | 19633837 |
| utility of a proposed csp typing nomenclature for australian aspergillus fumigatus isolates: identification of additional csp types and suggested modifications. | a recently developed csp typing scheme and proposed nomenclature was applied to a collection of 164 clinical and environmental aspergillus fumigatus isolates from melbourne, australia. fifteen csp variants were observed overall, including three that were not reported in the original nomenclature that described 19 csp variants, raising the possibility of phylogeographic differences between the australian and the previously studied european and north american a. fumigatus populations. however, tho ... | 2009 | 19524623 |
| stereospecific synthesis of aszonalenins by using two recombinant prenyltransferases. | in previous studies, two prenyltransferases were overproduced and characterised biochemically. anapt from neosartorya fischeri is involved in the biosynthesis of acetylaszonalenin and was shown to catalyse the c3-prenylation of (r)-benzodiazepinedione (6). cdpnpt from aspergillus fumigatus catalysed the n1-prenylation of different tryptophan-containing cyclic dipeptides. in this report, cdpnpt was found to catalyse the c3-prenylation of 6 and its (s)-isomer (7). interestingly, anapt and cdpnpt i ... | 2009 | 19421461 |
| evolution of aromatic prenyltransferases in the biosynthesis of indole derivatives. | a series of putative indole prenyltransferase genes could be identified in the genome sequences of different fungal strains including aspergillus fumigatus and neosartorya fischeri. the gene products show significant sequence similarities to dimethylallyltryptophan synthases from different fungi. we have cloned and overexpressed seven of these genes, fgapt1, fgapt2, ftmpt1, ftmpt2, 7-dmats, cdpnpt and anapt in escherichia coli and saccharomyces cerevisiae. the overproduced enzymes were character ... | 2009 | 19398116 |
| class ii alpha-mannosidase from aspergillus fischeri: energetics of catalysis and inhibition. | energetics of the catalysis of class ii alpha-mannosidase (e.c.3.2.1.24) from aspergillus fischeri was studied. the enzyme showed kcat/km for man (alpha1-3) man, man (alpha1-2) man and man (alpha1-6) man as 7488, 5376 and 3690 m(-1) min(-1), respectively. the activation energy, ea was 15.14, 47.43 and 71.21 kj/mol for alpha1-3, alpha1-2 and alpha1-6 linked mannobioses, respectively, reflecting the energy barrier in the hydrolysis of latter two substrates. the enzyme showed kcat/km as 3.56x10(5) ... | 2009 | 19038284 |
| fungal biodegradation of hard coal by a newly reported isolate, neosartorya fischeri. | cynodon dactylon (bermuda grass) has been observed to grow sporadically on the surface of coal dumps in the witbank coal mining area of south africa. root zone investigation indicated that a number of fungal species may be actively involved in the biodegradation of hard coal, thus enabling the survival of the plant, through mutualistic interaction, in this extreme environment. in an extensive screening program of over two thousand samples, the deuteromycete, neosartorya fischeri, was isolated an ... | 2008 | 19016511 |
| acetylaszonalenin biosynthesis in neosartorya fischeri. identification of the biosynthetic gene cluster by genomic mining and functional proof of the genes by biochemical investigation. | based on the structural information of acetylaszonalenin isolated from neosartorya fischeri, a putative biosynthetic gene cluster was identified in the genome sequence of this fungus by genomic mining. this cluster consists of three genes coding for a putative non-ribosomal peptide synthetase (anaps), a prenyltransferase (anapt), and an acetyltransferase (anaat). the coding sequences of anapt and anaat were cloned in pqe70 and pqe60, respectively, and overexpressed in escherichia coli. the solub ... | 2009 | 19001367 |
| fungal volatilization of trivalent and pentavalent arsenic under laboratory conditions. | production of volatile derivatives of arsenic was studied using pure cultures of different fungal strains under laboratory conditions. arsenic was used in its trivalent and pentavalent forms to evaluate the effect of arsenic valency on its biovolatilization. the average amount of volatilized arsenic for all fungal strains ranged from 0.026 mg to 0.257 mg and 0.024 mg to 0.191 mg of trivalent and pentavalent arsenic, respectively. these results show that approximately 23% of arsenic was volatiliz ... | 2009 | 18774290 |
| annotation of stress-response proteins in the aspergilli. | stress-response proteins of aspergillus nidulans, aspergillus clavatus, aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus oryzae, aspergillus terreus and neosartorya fischeri (3908 in total) were annotated and grouped according to stress types (http://193.6.155.82/aspergillusstress/). all genomes harboured elements of the sska-hoga/saka stress signalling pathway. there are accumulating data pointing at the importance of sska-hoga/saka signalling in different types of stre ... | 2009 | 18703157 |
| what can comparative genomics tell us about species concepts in the genus aspergillus? | understanding the nature of species" boundaries is a fundamental question in evolutionary biology. the availability of genomes from several species of the genus aspergillus allows us for the first time to examine the demarcation of fungal species at the whole-genome level. here, we examine four case studies, two of which involve intraspecific comparisons, whereas the other two deal with interspecific genomic comparisons between closely related species. these four comparisons reveal significant v ... | 2007 | 18490942 |
| genomic islands in the pathogenic filamentous fungus aspergillus fumigatus. | we present the genome sequences of a new clinical isolate of the important human pathogen, aspergillus fumigatus, a1163, and two closely related but rarely pathogenic species, neosartorya fischeri nrrl181 and aspergillus clavatus nrrl1. comparative genomic analysis of a1163 with the recently sequenced a. fumigatus isolate af293 has identified core, variable and up to 2% unique genes in each genome. while the core genes are 99.8% identical at the nucleotide level, identity for variable genes can ... | 2008 | 18404212 |
| optimization of biobleaching of paper pulp in an expanded bed bioreactor with immobilized alkali stable xylanase by using response surface methodology. | purified alkali stable xylanase from aspergillus fischeri was immobilized on polystyrene beads using diazotization method. an expanded bed bioreactor was developed with these immobilized beads to biobleach the paper pulp in continuous mode. response surface methodology was applied to optimize the biobleaching conditions. temperature (degrees c), flow rate of pulp (ml/min) and concentration of the pulp (%) were selected as variables in this study. optimal conditions for biobleaching process were ... | 2008 | 18343104 |
| cloning, characterization and expression of the extracellular lipase gene from aureobasidium pullulans hn2-3 isolated from sea saltern. | the extracellular lipase structural gene was isolated from cdna of aureobasidium pullulans hn2-3 by using smart(tm) race cdna amplification kit. the gene had an open reading frame of 1245 bp long encoding a lipase. the coding region of the gene was interrupted by only one intron (55 bp). it encodes 414 amino acid residues of a protein with a putative signal peptide of 26 amino acids. the protein sequence deduced from the extracellular lipase structural gene contained the lipase consensus sequenc ... | 2008 | 18340544 |
| weed growth inhibitors from aspergillus fischeri tistr 3272. | chloroform and ethyl acetate extracts of aspergillus fischeri tistr 3272 showed good growth inhibitory activity on mimosa pigra and echinochloa crus-galli. bioassay-directed fractionation of the active extracts led to the isolation of five known compounds, (+)-terrein (1), (-)-6-hydroxymellein (2), two diketopiperazines (cyclo-(s-pro-s-leu) (3) and cyclo-(s-pro-s-val) (4)) and butyrolactone i (5). compounds 2-5 were reported for the first time in this fungus. their structural determinations were ... | 2007 | 18075891 |
| a 7-dimethylallyltryptophan synthase from aspergillus fumigatus: overproduction, purification and biochemical characterization. | a putative prenyltransferase gene, afu3g12930, was identified in the genome sequence of aspergillus fumigatus. eal92290, encoded by afu3g12930, consists of 472 aa, with a molecular mass of about 53 kda. the coding sequence of afu3g12930 was cloned in pqe60, and overexpressed in escherichia coli. the soluble his(6)-fusion protein was purified to apparent homogeneity, and characterized biochemically. the enzyme was found to catalyse the prenylation of trp at the c-7 position of the indole moiety, ... | 2007 | 17906140 |
| fluorescence quenching and time-resolved fluorescence studies of alpha-mannosidase from aspergillus fischeri (ncim 508). | apart from the vital role in glycoprotein biosynthesis and degradation, alpha-mannosidase is currently an important therapeutic target for the development of anticancer agents. fluorescence quenching and time-resolved fluorescence of alpha-mannosidase, a multitryptophan protein from aspergillus fischeri were carried out to investigate the tryptophan environment. the tryptophans were found to be differentially exposed to the solvent and were not fully accessible to the neutral quencher indicating ... | 2007 | 17849180 |
| dna sequence characterization and molecular evolution of mat1 and mat2 mating-type loci of the self-compatible ascomycete mold neosartorya fischeri. | degenerate pcr and chromosome-walking approaches were used to identify mating-type (mat) genes and flanking regions from the homothallic (sexually self-fertile) euascomycete fungus neosartorya fischeri, a close relative of the opportunistic human pathogen aspergillus fumigatus. both putative alpha- and high-mobility-group-domain mat genes were found within the same genome, providing a functional explanation for self-fertility. however, unlike those in many homothallic euascomycetes (pezizomycoti ... | 2007 | 17384199 |
| the first filamentous fungal genome sequences: aspergillus leads the way for essential everyday resources or dusty museum specimens? | the published aspergillus genome sequences (a. nidulans, a. fumigatus, a. oryzae) and further sequence data from a. clavatus, neosartorya fischeri, a. flavus, a. niger, a. parasiticus and a. terreus are the first from a group of related filamentous fungi. they indicate the gains possible from genomic approaches, but also problems that arise after the sequences are finished. benefits include a greater understanding of genome structure and evolution, insights into gene regulation, predictions of n ... | 2007 | 17185529 |
| two families of extracellular phospholipase c genes are present in aspergilli. | fungi secrete extracellular enzymes to enable them to harvest nutrients from the environment. in the case of pathogenic fungi these enzymes can also be pathogenesis factors. here we report the identification in fungi of a complex family of extracellular phospholipase c (plc) enzymes, homologous to the pseudomonas aeruginosa plch_pseae. database searches and phylogenetic analysis showed that the plcs clustered into two groups with different evolutionary histories. one group, subdivided into plc-a ... | 2006 | 17015001 |
| the chemistry of mould tissue: factors influencing the amount and nature of the fat produced by aspergillus fischeri. | 1935 | 16745649 | |
| the chemistry of mould tissue: fractionation of the nitrogen in the mycelium of aspergillus fischeri. | 1934 | 16745412 |