Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| occurrence of non-lactic acid bacteria populations involved in protein hydrolysis of cold-stored high moisture mozzarella cheese. | the aim of this study was to analyse non-lactic acid bacteria populations (nlabps) and evaluate their role in proteolysis of cold-stored high moisture (hm) mozzarella cheese. nlabps reached values close to 8 log cfu ml(-1) after seven days of cold storage. sequencing of 16 rdna and rpob genes and molecular biotyping allowed to identify 66 bacterial strains belonging to 25 species from 15 genera, mainly represented by pseudomonas, acinetobacter, and rahnella. fifteen strains showed proteolytic ac ... | 2012 | 22265281 |
| maple sap predominant microbial contaminants are correlated with the physicochemical and sensorial properties of maple syrup. | maple sap processing and microbial contamination are significant aspects that affect maple syrup quality. in this study, two sample sets from 2005 and 2008 were used to assess the maple syrup quality variation and its relationship to microbial populations, with respect to processing, production site and harvesting period. the abundance of maple sap predominant bacteria (pseudomonas fluorescens group and two subgroups, rahnella spp., janthinobacterium spp., leuconostoc mesenteroides) and yeast (m ... | 2011 | 22236761 |
| gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities. | dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ... | 2012 | 22234511 |
| exploring new strains of dye-decolorizing bacteria. | this study unveiled a new strategy to explore new indigenous strains with excellent decolorization capabilities from freshwaters and seawaters. two new bacterial decolorizers dx2b and sh7b, which have the capability to decolorize textile dyes, were isolated from cross-strait taiwan and china. according to pcr-augmented 16s rrna gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to type strains rahnella aquatilis, acinetobacter guillouiae, mic ... | 2011 | 22178338 |
| Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria. | The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ... | 2011 | 22163022 |
| freezing and freeze-drying of the bacterium rahnella aquatilis bnm 0523: study of protecting agents, rehydration media and freezing temperatures. | the aim of the present study was to evaluate and compare freezing and freeze-drying treatments for conserving rahnella aquatilis (bnm 0523) with the goal to achieve an adequate commercial formulation of this biocontrol agent. | 2011 | 21913947 |
| diversity and community structure of culturable arsenic-resistant bacteria across a soil arsenic gradient at an abandoned tungsten-tin mining area. | we studied the bacterial diversity at a single location (the terrubias mine; salamanca province, spain) with a gradient of soil as contamination to test if increasing levels of as would (1) change the preponderant groups of arsenic-resistant bacteria and (2) increase the tolerance thresholds to arsenite [as(iii)] and arsenate [as(v)] of such bacteria. we studied the genetic and taxonomic diversity of culturable arsenic-resistant bacteria by pcr fingerprinting techniques and 16s rrna gene sequenc ... | 2011 | 21724233 |
| monitoring the bacterial community of maize silage stored in a bunker silo inoculated with enterococcus faecium, lactobacillus plantarum and lactobacillus buchneri. | to monitor variations in the bacterial community and fermentation products of maize silage within and between bunker silos. | 2011 | 21447012 |
| extended-spectrum beta-lactamase gene sequences in gram-negative saprophytes on retail organic and nonorganic spinach. | a substantial proportion of infections caused by drug-resistant gram-negative bacteria (gnb) in community and health care settings are recognized to be caused by evolutionarily related gnb strains. their global spread has been suggested to occur due to human activities, such as food trade and travel. these multidrug-resistant gnb pathogens often harbor mobile drug resistance genes that are highly conserved in their sequences. because they appear across different gnb species, these genes may have ... | 2011 | 21216903 |
| bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri. | to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage. | 2011 | 21204884 |
| cold-adapted and rhizosphere-competent strain of rahnella sp. with broad-spectrum plant growth-promotion potential. | a phosphate-solubilizing bacterial strain isolated from hippophae rhamnoides rhizosphere was identified as rahnella sp. based on its phenotypic features and 16s rrna gene sequence. the bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of inorganic and organic phosphate solubilization, 1-aminocyclopropane-1- carboxylate-deaminase activity, ammonia generation, and siderophore production. the strain also produced indol ... | 2010 | 21193830 |
| genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan. | bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ... | 2010 | 21193815 |
| spoilage-related microbiota associated with chilled beef stored in air or vacuum pack. | in order to study the spoilage-related microbiota of beef at species level, a combination of culture-independent and culture-dependent methods was used to analyse nine different beef samples stored at 4°c in air or in vacuum pack. plate counts on selective agars after 0, 7 and 20 days of storage showed that vacuum packaging reduced the viable counts of brochothrix thermosphacta, pseudomonas spp. and enterobacteriaceae, whereas the growth of lactic acid bacteria (lab) was unaffected. storage in v ... | 2010 | 21056779 |
| occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions. | the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ... | 2010 | 21033566 |
| identification of cis- and trans-acting elements in phw126, a representative of a novel group of rolling circle plasmids. | phw126, pigrk, pigms31 and prao1 are the only known members of a novel and as yet uncharacterised family of rolling circle plasmids. phw126 contains only two open reading frames, of which one shows homology to pmv158-family mobilisation proteins. here we provide evidence that the second open reading frame encodes a replication protein (rep). mutation or deletion of this gene resulted in replication deficient constructs, but providing functional rep from a compatible vector rescued these construc ... | 2011 | 20854841 |
| production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages. | histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ... | 2010 | 20673614 |
| infection caused by rahnella aquatilis. | the authors report a case of a 27-year-old african american woman with sickle cell disease who developed septic shock caused by a rahnella aquatilis infection associated with a peripherally inserted central venous catheter. the infection was treated successfully with ciprofloxacin. r aquatilis is a gram-negative rod, first isolated from freshwater in 1976, which has been linked to human disease in rare instances, most commonly in immunosuppressed individuals. in addition to this case report, the ... | 2010 | 20545014 |
| comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis. | detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ... | 2010 | 20526836 |
| rahn-2, a chromosomal extended-spectrum class a beta-lactamase from rahnella aquatilis. | rahnella aquatilis is an environmental enterobacterial species with a chromosomal bla(rahn-1) gene encoding extended-spectrum class a beta-lactamase rahn-1. we describe the diversity of bla(rahn) genes from two groups of strains, g1 and g2, isolated from raw fruits and vegetables, and the new class a beta-lactamase rahn-2. | 2010 | 20511369 |
| [comparative characterisation of lipopolysaccharides of rahnella aquatills]. | lipopolysaccharides (lps) of eight strains of r. aquatilis isolated from different sources have been studied. the studies of neutral monosaccharide composition evidence that all of lps contain galactose (13.4-68.5%), glucose (5.7-29.8%) and heptose (2.6-8.3%) (depending on strains). some monosaccharides, such as ribose (95u007), rhamnose (95u011, 95u012, 96u036), fucose (95u003, 95u004, 95u007) and mannose (95u012, 96u035, 96u036, 96u037) were absent in lps. arabinose was present in two strains- ... | 2009 | 20455427 |
| [chemical characterisation of structural components of rahnella aquatilis lipopolysaccharides]. | the studies of lipopolysaccharides (lps) of eight rahnella aquatilis strains, isolated from different sources have shown that they contain both s- and r-types of molecules. this fact is evidenced by the presence of high-molecular fraction of o-specific polysaccharides (o-ps) and low-molecular fraction of core oligosaccharides. the predominant monosaccharides of core oligosaccharides were glucose, galactose. the presence of only one high-molecular fraction o-ps was a characteristic feature of all ... | 2010 | 20364711 |
| seasonal and regional diversity of maple sap microbiota revealed using community pcr fingerprinting and 16s rrna gene clone libraries. | an arbitrary primed community pcr fingerprinting technique based on capillary electrophoresis was developed to study maple sap microbial community characteristics among 19 production sites in québec over the tapping season. presumptive fragment identification was made with corresponding fingerprint profiles of bacterial isolate cultures. maple sap microbial communities were subsequently compared using a representative subset of 13 16s rrna gene clone libraries followed by gene sequence analysis. ... | 2010 | 20202776 |
| frequency and diversity of small cryptic plasmids in the genus rahnella. | rahnella is a widely distributed genus belonging to the enterobacteriaceae and frequently present on vegetables. although rahnella has interesting agro-economical and industrial properties and several strains possess antibiotic resistances and toxin genes which might spread within microbial communities, little is known about plasmids of this genus. thus, we isolated a number of rahnella strains and investigated their complements of small plasmids. | 2010 | 20170524 |
| 16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens. | a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ... | 2009 | 19937215 |
| organic and conventional fruits and vegetables contain equivalent counts of gram-negative bacteria expressing resistance to antibacterial agents. | resistance to antibiotics is a major public health problem which might culminate in outbreaks caused by pathogenic bacteria untreatable by known antibiotics. most of the genes conferring resistance are acquired horizontally from already resistant commensal or environmental bacteria. food contamination by resistant bacteria might be a significant source of resistance genes for human bacteria but has never been precisely assessed, nor is it known whether organic products differ in this respect fro ... | 2010 | 19919536 |
| microbial diversity changes in soybean sprouts treated with enterocin as-48. | seed sprouts may act as vehicles for foodborne pathogenic bacteria. in the present study, the effect of washing treatment with the enterococcal bacteriocin enterocin as-48 on the microbiota of two batches of soybean sprouts was studied by culture-dependent and independent methods throughout storage at 10 degrees c. viable cell counts of bacteriocin-treated samples revealed some modifications only for lactic acid bacteria and enterococci during storage. in the control samples from batch 1, the cu ... | 2009 | 19835782 |
| mutations that disrupt either the pqq or the gdh gene of rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall. | rahnella aquatilis hx2, a biocontrol agent for grapevine crown gall caused by agrobacterium vitis, produces an antibacterial substance that inhibits the growth of a. vitis in vitro. in this study, we show that mh15 and mh16, two tn5-induced mutants of hx2, have lost their abilities to inhibit a. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single tn5 insertions. they are also impaired in producing pyrroloquinolin ... | 2009 | 19734331 |
| bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae). | the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ... | 2009 | 19543937 |
| role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of brassica juncea. | in this study, we have shown that the plant growth promoting bacterial strain nbri k24 and strain nbri k3 from fly ash (fa) contaminated soil reduce the toxicity of ni and cr in brassica juncea (indian mustard) and promote plant growth under pot culture experiments. isolated strains nbri k24 and nbri k3 were characterized based on the 16s rdna sequencing and identified as enterobacter aerogenes and rahnella aquatilis respectively. both the strains were siderophore producing and found capable of ... | 2009 | 19487076 |
| biogeochemical changes induced in uranium mining waste pile samples by uranyl nitrate treatments under anaerobic conditions. | response of the subsurface soil bacterial community of a uranium mining waste pile to treatments with uranyl nitrate over different periods of time was studied under anaerobic conditions. the fate of the added u(vi) without supplementation with electron donors was investigated as well. by using 16s rrna gene retrieval, we demonstrated that incubation with uranyl nitrate for 4 weeks resulted in a strong reduction in and even disappearance of some of the most predominant bacterial groups of the or ... | 2009 | 19476503 |
| enterobacteriaceae facilitate the anaerobic degradation of glucose by a forest soil. | anoxic micro zones that occur in soil aggregates of oxic soils may be temporarily extended after rainfall and thus facilitate the anaerobic degradation of organic compounds in soils. the microbial degradation of glucose by anoxic slurries of a forest soil yielded acetate, co2, h2, succinate, and ethanol, products indicative of mixed acid fermentation. prokaryotes involved in this process were identified by time-resolved 16s rrna gene-targeted stable isotope probing with [13c-u]-glucose. all labe ... | 2009 | 19453494 |
| isolation and structure elucidation of two different polysaccharides from the lipopolysaccharide of rahnella aquatilis 33071t. | two different polysaccharides were obtained by mild acid degradation of the lipopolysaccharide of rahnella aquatilis 33071(t). these were studied by sugar and methylation analyses along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structures were established for the polysaccharides: -->4)-alpha-d-rhap-(1-->3)-alpha-d-rhap-(1-->3)beta-d-galp-(1-->3)-alpha-d-manp-(1-->2)-alpha-d-manp-(1--> beta-d-glcp-(1-->3)-alpha-d-galp-(1-->4)-alpha-d-glcpa-(1-->2). the former structure is new, ... | 2009 | 19442965 |
| six cases of sepsis caused by pantoea agglomerans in a teaching hospital. | pantoea agglomerans is an environmental organism which may seldom cause opportunistic infections. here we report on a 6 case outbreak in a teaching hospital. within three months . agglomerans was isolated from blood cultures of 5 patients from oncology and 1 patient from icu departments. p. agglomerans was in pure culture in 5 cases, while in the last one rahnella aquatilis and candida famata were also isolated. therefore, p. agglomerans is able to produce nosocomial infections in patients with ... | 2009 | 19382678 |
| brenneria salicis, the bacterium causing watermark disease in willow, resides as an endophyte in wood. | brenneria salicis has been studied in willow wood only in relation to watermark disease. in this pathogenic condition, the bacterium occurs at high concentrations. pathogenicity of b. salicis is still uncontrollable and the disease unpredictable because the plant-bacteria interaction is not understood. thanks to molecular techniques b. salicis can be detected at low concentrations, which are found in most non-pathogenic interactions. brenneria salicis was identified and traced with a new specifi ... | 2009 | 19220404 |
| an efficient plasmid vector for constitutive high-level expression of foreign genes in escherichia coli. | the levansucrase gene (lsra) from rahnella aquatilis was strongly expressed in a constitutive manner in escherichia coli when cloned into a pbluescript ks-based prl1cp plasmid vector. the native promoter upstream of lsra and the lacz promoter cooperatively enhanced the expression of lsra to a level that was comparable to that of the t7 promoter, which is used in commercial pet expression vector system. a putative rho-independent transcription termination signal downstream of lsra was crucial for ... | 2009 | 19214389 |
| universal and species-specific bacterial 'fungiphiles' in the mycospheres of different basidiomycetous fungi. | in previous work, several bacterial groups that show a response to fruiting bodies (the mycosphere) of the ectomycorrhizal fungus laccaria proxima were identified. we here extend this work to a broader range of fungal fruiting bodies sampled at two occasions. pcr-dgge analyses showed clear effects of the mycosphere of diverse fungi on the total bacterial and pseudomonas communities in comparison with those in the corresponding bulk soil. the diversities of the pseudomonas communities increased d ... | 2009 | 19196267 |
| potential of bulb-associated bacteria for biocontrol of hyacinth soft rot caused by dickeya zeae. | dickeya zeae is a pectinolytic bacterium responsible for soft rot disease in flower bulb crops. in this study, the possibility of controlling soft rot disease in hyacinth by using antagonistic bacteria isolated from hyacinth bulbs was explored. | 2009 | 19054227 |
| dynamics of phosphorus and phytate-utilizing bacteria during aerobic degradation of dairy cattle dung. | during organic wastes degradation, p is transformed which may affect its availability. in this study, the dynamics of p and the occurrence of phytate-utilizing bacteria (pub) were evaluated during aerobic degradation of dairy cattle dung in laboratory-scale reactors for 105 d. the results showed an increase of water-soluble inorganic p (pi) (from 570 to 1890 mg kg(-1)) and biomass p (from 390 to 870 mg kg(-1)) during the initial 40 d. after this period, water-soluble pi remained constant (around ... | 2009 | 18977014 |
| occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet. | abstract a relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (iaa) in culture media supplemented with tryptophan. while over 50% of the strains produced at least small amounts of iaa in culture, about 25% of the strains exhibited high iaa production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. a majority of the strains that produced high amounts of iaa ... | 1998 | 18944847 |
| [chemical characteristics and endotoxic activity of the lipopolysaccharide of rahnella aquatilis 2-95]. | the lipopolysaccharide (lps) from a new enterobacteriaceae species, rahnella aquatilis 2-95, was isolated and investigated. the structural components of the lps molecule, namely, lipid a, core oligosaccharide, and o-specific polysaccharide, were obtained by mild acid hydrolysis. in lipid a, 3-oxytetradecanoic and tetradecanoic acids were found to be the predominant fatty acids. the major monosaccharides of the core oligosaccharide were galactose, arabinose, fucose, rhamnose, and an unidentified ... | 2008 | 18683651 |
| structure of the o-specific polysaccharide of the lipopolysaccharide of rahnella aquatilis 95 u003. | the o-polysaccharide of rahnella aquatilis 95 u003 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, smith degradation and (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, h-detected (1)h,(13)c hsqc and hmqc-tocsy experiments. the o-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [carbohydrate structure: see text] | 2008 | 18674754 |
| [glycopolymers of microorganisms: molecular relationship between structure, function, and biological activity]. | the results of the department of biochemistry investigations during the recent 5 years are presented. for the first time the structures of o-specific polysaccharides of lipopolysaccharides of representatives of enterobacteriaceae new genus--rahnella aquatilis have been established. the structures are represented by linear or branched mono-, tri- and hexasaccharides. as well as in other enterobacteriaceae representatives only one hydrohylated acid--3-hydroxytetradecanoic has been established in l ... | 2008 | 18663920 |
| enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing. | in the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ... | 2008 | 18493013 |
| binding of nontarget microorganisms from food washes to anti-salmonella and anti-e. coli o157 immunomagnetic beads: most probable composition of background eubacteria. | we present herein the composition of bacterial communities occurring in ground chicken and the changes which arise in these populations based upon nonselective partitioning by commercially-available dynal anti-salmonella and anti-e. coli o157 immunomagnetic beads (imb). our enumeration and colony selection protocol was based upon a 6 × 6 drop plate method (n = 18 for each 25-g sub-sampling) using a dilution which resulted in ca. 4-8 colonies per drop. an average of 82 ± 13 colonies were selected ... | 2008 | 18311564 |
| analysis of the intestinal microflora in hepialus gonggaensis larvae using 16s rrna sequences. | gut microbial diversity provides insight into the basic function of a gut microbial ecosystem. in this study, restriction fragment length polymorphism 16s rrna sequences was used to detect the intestinal microbial diversity of hepialus gonggaensis larvae. the total dna of microorganisms was extracted from the intestinal contents and 16s rrna was amplified. a nearly full-length of 16s rrna sequence library was constructed. the fingerprints of the microorganisms were analyzed by isolating plasmid ... | 2008 | 18183461 |
| aerobic uranium (vi) bioprecipitation by metal-resistant bacteria isolated from radionuclide- and metal-contaminated subsurface soils. | in this study, the immobilization of toxic uranium [u(vi)] mediated by the intrinsic phosphatase activities of naturally occurring bacteria isolated from contaminated subsurface soils was examined. the phosphatase phenotypes of strains belonging to the genera, arthrobacter, bacillus and rahnella, previously isolated from subsurface soils at the us department of energy's (doe) oak ridge field research center (orfrc), were determined. the orfrc represents a unique, extreme environment consisting o ... | 2007 | 17991039 |
| possible involvement of psychrotolerant enterobacteriaceae in blown pack spoilage of vacuum-packaged raw meats. | recent investigations of blown pack spoilage in new zealand chilled vacuum-packaged meats have found moderate to high numbers of enterobacteriaceae in the spoilage flora, but no clostridia, such as c. estertheticum and c. gasigenes, that are usually associated with blown pack spoilage. this study showed that pyschrotolerant enterobacteriaceae produced gas in a lamb homogenate model under anaerobic conditions and that these organisms could cause blown pack spoilage of vacuum-packaged chilled meat ... | 2007 | 17870199 |
| atrazine degradation by aerobic microorganisms isolated from the rhizosphere of sweet flag (acorus calamus l.). | in presented study the capability of microorganisms isolated from the rhizosphere of sweet flag (acorus calamus) to the atrazine degradation was assessed. following isolation of the microorganisms counts of psychrophilic bacteria, mesophilic bacteria and fungi were determined. isolated microorganisms were screened in terms of their ability to decompose a triazine herbicide, atrazine. our results demonstrate that within the rhizosphere of sweet flag there were 3.8x10(7) cfu of psychrophilic bacte ... | 2008 | 17594524 |
| biological control of postharvest spoilage caused by penicillium expansum and botrytis cinerea in apple by using the bacterium rahnella aquatilis. | the epiphytic bacterium rahnella aquatilis, isolated from fruit and leaves of apples, was tested for antagonistic properties against penicillium expansum and botrytis cinerea on red delicious apple fruit. in "in vitro" assays, this bacterium inhibited completely the germination of p. expansum and b. cinerea spores, but it needed direct contact with the spores to do it. however the putative mechanism seemed be different for the two pathogens. the bacterium did not produce extracellular antibiotic ... | 2007 | 17007950 |
| isolation and characterization of cold-adapted strains producing beta-galactosidase. | beta-galactosidase is extensively employed in the manufacture of dairy products, including lactose-reduced milk. here, we have isolated two gram-negative and rod-shaped coldadapted bacteria, bs 1 and hs 39. these strains were able to break down lactose at low temperatures. although two isolates were found to grow well at 10 degrees , the bs 1 strain was unable to grow at 37 degrees . another strain, hs-39, evidenced retarded growth at 37 degrees . the biochemical characteristics and the results ... | 2006 | 16953174 |
| volatile metabolite production of spoilage micro-organisms on a mixed-lettuce agar during storage at 7 degrees c in air and low oxygen atmosphere. | this paper describes the volatile metabolite production of spoilage bacteria (pantoea agglomerans and rahnella aquatilis) and spoilage yeasts (pichia fermentans and cryptococcus laurentii), previously isolated from mixed lettuce, on a simulation medium of shredded mixed lettuce (mixed-lettuce agar) both under air conditions and modified atmosphere (ma)-conditions at 7 degrees c. these latter conditions simulated the equilibrium modified atmosphere packaging, which is used to extend the shelf-lif ... | 2006 | 16860421 |
| isolation and characterization of phw15, a small cryptic plasmid from rahnella genomospecies 2. | a small cryptic plasmid designated phw15 was isolated from rahnella genomospecies 2 wmr15 and its complete nucleotide sequence was determined. the plasmid contained 3002 bp with a g+c content of 47.4%. the origin of replication was identified by deletion analysis as a region of about 600 bp. this region had an identity of 70% to the replication origin of the cole1 plasmid at the nucleotide level. sequence analysis revealed the typical elements: rna i, rna ii and their corresponding promoters, a ... | 2006 | 16844220 |
| changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. | the microbial spoilage of beef was monitored during storage at 5 degrees c under three different conditions of modified-atmosphere packaging (map): (i) air (map1), (ii) 60% o2 and 40% co2 (map2), and (iii) 20% o2 and 40% co2 (map3). pseudomonas, enterobacteriaceae, brochothrix thermosphacta, and lactic acid bacteria were monitored by viable counts and pcr-denaturing gradient gel electrophoresis (dgge) analysis during 14 days of storage. moreover, headspace gas composition, weight loss, and beef ... | 2006 | 16820458 |
| endobacteria in some ectomycorrhiza of scots pine (pinus sylvestris). | the diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (suillus flavidus, suillus variegatus, russula paludosa and russula sp.) of scots pine (pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of pcr-amplified rdna intergenic spacer regions and by sequence analyses of 16s rrna genes. ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. bacterial communities from s ... | 2006 | 16542403 |
| compositions of maple sap microflora and collection system biofilms evaluated by scanning electron microscopy and denaturing gradient gel electrophoresis. | the bacterial microflora of maple sap and biofilms in collection system tubing were studied through the use of bacterial counts, scanning electron microscopy (sem) of surfaces and the analysis of 16s rrna gene by denaturing gradient gel electrophoresis (dgge). samples were taken at five times during the 2002 and 2003 seasons in order to follow the changes in the microflora of this complex ecosystem. bacterial counts showed the growth of bacterial populations as the season advanced. these populat ... | 2006 | 16515815 |
| the efficacy of electrolysed oxidising water for inactivating spoilage microorganisms in process water and on minimally processed vegetables. | the efficacy of electrolysed oxidising water (eow) for inactivating spoilage microorganisms in process water and on minimally processed vegetables was investigated. the direct effect of eow on three important spoilage bacteria namely; pseudomonas fluorescens, pantoea agglomerans or rahnella aquatilis was determined by inoculating tap water or "artificial process water" with approximately 8 log cfu/ml pure culture and electrolysing the resultant solutions. the three bacteria were each reduced to ... | 2006 | 16504328 |
| pseudomonas, the dominant polycyclic aromatic hydrocarbon-degrading bacteria isolated from antarctic soils and the role of large plasmids in horizontal gene transfer. | twenty-two polycyclic aromatic hydrocarbon (pah)-degrading bacterial strains were isolated from antarctic soils with naphthalene or phenanthrene as a sole carbon source, while no degrader was obtained from an unpolluted sampling site. phylogenetic analysis showed that all belonged to the genus pseudomonas except one that was identified as the genus of rahnella. some of them were closely related to previously reported cold-tolerant species, while some were separated in deeply rooted branches and ... | 2006 | 16478452 |
| structures of two putative o-specific polysaccharides from the rahnella aquatilis 3-95 lipopolysaccharide. | two polysaccharide preparations (opsi and opsii) were obtained by mild acid degradation of the lipopolysaccharide of rahnella aquatilis 3-95. studies by chemical methods and 1h and 13c nmr spectroscopy showed that opsi is a linear alpha-d-mannan having a trisaccharide repeat and opsii is a approximately 2:1 mixture of the same mannan and an alpha-d-glucan: | 2006 | 16313891 |
| biological control of bacterial spot of tomato caused by xanthomonas campestris pv. vesicatoria by rahnella aquatilis. | xanthomonas campestris pv. vesicatoria strain 2 was isolated from infected tomato seedlings grown in open field in egypt. this strain produced irregular yellow-necrotic areas on tomato leaves and spotting of the stem. in an attempt to control this disease biologically, four experiments were conducted and tomato seedlings were pretreated, before the pathogen, with either of two antagonistic strains of rahnella aquatilis through leaves, roots, soil or seeds. in all experiments, seedlings pretreate ... | 2005 | 16255138 |
| production of a novel exopolysaccharide by rahnella aquatilis. | a bacterium tomakomai b-1 that was isolated from the activated sludge of a treatment process for wastewater of the pulp industry was found to produce soluble and insoluble exopolysaccharides (epss) when grown aerobically in a medium containing sucrose as the sole carbon source. the sugar component of soluble epss consisted of rhamnose, mannose, galactose, glucose, mannuronic acid, galacturonic acid and glucuronic acid, and that of insoluble epss consisted of rhamnose, mannose, galactose, glucose ... | 1999 | 16232447 |
| [characterization of the lipopolysaccharide from rahnella aquatilis 1-95]. | the lipopolysaccharide from the freshwater bacterium rahnella aquatilis 1-95 has been isolated and investigated for the first time. the structural components of the lipopolysaccharide molecule: lipid a, core oligosaccharide, and o-specific polysaccharide were isolated by mild acidic hydrolysis. in lipid a, 3-hydroxytetradecanoic and tetradecanoic acids were found to be the predominant fatty acids. in the core oligosaccharide, galactose, arabinose, fucose, and an unidentified component were shown ... | 2005 | 16211849 |
| gram-negative bacteria from the camel tick hyalomma dromedarii (ixodidae) and the chicken tick argas persicus (argasidae) and their antibiotic sensitivities. | a total of nine species of gram-negative bacteria were isolated from organs and haemolymph of the hard tick hyalomma (hyalomma) dromedarii and the soft tick argas (persicargas) persicus. four species namely serratia liquefaciens, stenotrophomonas maltophilia, klebsiella ornithinolytica and aeromonas hydrophila were isolated from h. dromedarii and five species namely rahnella aquatilis, pseudomonas fluorescens, enterobacter cloacae, chryseomonas luteola and chryseobacterium meningosepticum were i ... | 2005 | 15880998 |
| rahnella aquatilis bacteremia from a suspected urinary source. | a 76-year-old male with prostatic hyperplasia presented with acute pyelonephritis. blood cultures yielded rahnella aquatilis. treatment with intravenous followed by oral levofloxacin resulted in cure. important characteristics of this organism include its biochemical similarities to enterobacter agglomerans, its apparent ability to cause bacteremia from a renal focus, and its response to quinolone therapy. | 2005 | 15872303 |
| bacterial endophytes from seeds of norway spruce (picea abies l. karst). | endophytic bacteria from wooden plants and especially seed-associated endophytes are not well studied. fresh seeds collected from four norway spruce trees (picea abies) from different locations in the slovene subalpine region were surface-sterilised and dissected into a seed coat, embryo and endosperm. the presence of endophytes was detected by culturing methods and by direct amplification of the eubacterial 16s rdna gene. both approaches identified bacteria from genera pseudomonas and rahnella ... | 2005 | 15766788 |
| [biological activity of native and modified lipopolysaccharides of rahnella aquatilis]. | modified lipopolysaccharide (lps) preparations: dephosphorylated and n,o-dcacylated ones of six strains of rahnella aquatilis have been obtained. it has been shown that the modified lps, in contrast to native ones, have lost completely their toxicity and pathogenicity. this can evidence for the participation of both acyl, and phosphoryl groups in biological activity of lps of r. aquatilis. | 2004 | 15765868 |
| coliform and pathologic bacteria in sierra nevada national forest wilderness area lakes and streams. | to analyze backcountry-area water quality in us department of agriculture forest service-designated wilderness areas for the presence of coliform and potentially pathogenic bacteria. | 2004 | 15636374 |
| an analysis of wilderness water in kings canyon, sequoia, and yosemite national parks for coliform and pathologic bacteria. | to determine the prevalence of coliform and potentially pathogenic bacteria in remote backcountry alpine lakes and streams of national parks in the sierra nevada mountains. | 2004 | 15636373 |
| identification of enterobacteriaceae from washed and unwashed commercial shell eggs. | to evaluate the effect of processing on the safety and quality of retail shell eggs, a storage study was conducted with unwashed and commercially washed eggs. this work demonstrated that commercial processing decreased microbial contamination of eggshells. to know which species persisted during storage on washed or unwashed eggs, enterobacteriaceae isolates were selected and identified biochemically. for each of three replications, shell eggs were purchased from a commercial processing plant, tr ... | 2004 | 15553650 |
| inhibition of the reattachment of young adult zebra mussels by single-species biofilms and associated exopolymers. | to determine the effects of single-species bacterial films and their associated extracellular products on the reattachment of young adult zebra mussels. | 2004 | 15546415 |
| [characteristics of structure components of rahnella aquatilis lipopolysaccharides]. | lps have been isolated from 6 strains of rahnella aquatilis, their separate structure components have been obtained: lipid a, o-specific polysaccharide shain (ops) and core oligosaccharide. 3-oxytetradecanoic (31.7%) and tetradecanoic (17.8%) acids are dominating fatty acids of the lipid a. the core oligosaccharide of most investigated strains includes galactose and glucose, some of them include arabinose and ribose. glucose, mannose and galactose are dominating monosaccharides in the compositio ... | 2004 | 15515896 |
| structure of the o-polysaccharide of the lipopolysaccharide of rahnella aquatilis 1-95. | the o-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of rahnella aquatilis 1-95 and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including noesy and 1h,13c hsqc experiments for linkage and sequence analysis. the following structure of the branched trisaccharide repeating unit of the o-polysaccharide was established: [carbohydrate structure: see text]. | 2004 | 15220091 |
| [isolation and characterization of rahnella aquatilis lipopolysaccharides]. | lipopolysaccharides (lps) of six strains of rahnella aquatilis isolated from freshwater reservoirs, plant rhizosphere, and from patient have been investigated. it was shown, that lps of type strain was characterized by the presence of rhamnose and mannose as predominant monosaccharides, while lps of the rest strains contained galactose and fucose. the glucosamine content was insignificant (0.42-1.0%) in lps of all strains studied. it was shown by double immunodiffusion in agar, immunoelectrophor ... | 2004 | 15208851 |
| antitumor activity of levan polysaccharides from selected microorganisms. | levans were isolated from the cultures of gluconoacetobacter xylinus (g-levan; mw = 40,000), microbacterium laevaniformans (m; mw = 710,000), rahnella aquatilis (r; mw = 380,000), and zymomonas mobilis (z; mw = 570,000). the levans were composed mainly of fructose residues when analyzed by tlc and hplc, and their main backbones were beta-(2,6)-linkages with beta-(2,1)-branches by gc-ms and nmr. in the in vitro antitumor activity test of the levans against eight different tumor cell lines, relati ... | 2004 | 15178007 |
| -1 frameshifting at a cga aag hexanucleotide site is required for transposition of insertion sequence is1222. | the discovery of programmed -1 frameshifting at the hexanucleotide shift site cga_aag, in addition to the classical x_xxy_yyz heptanucleotide shift sequences, prompted a search for instances among eubacterial insertion sequence elements. is1222 has a cga_aag shift site. a genetic analysis revealed that frameshifting at this site is required for transposition. | 2004 | 15126494 |
| characterization of two antagonistic strains of rahnella aquatilis isolated from soil in egypt. | in an attempt to obtain biological control agents for controlling bacterial spot of cucumber, over 250 bacterial strains were isolated from agricultural soil samples, collected from different localities in giza governorate (egypt) and screened for in vitro antibiosis towards xanthomonas campestris. only 2 strains showed antagonistic activity. they and their culture filtrates restricted the growth of a number of g- and g(+)-indicator bacteria. on chrome azurol s agar, both strains exhibited a mar ... | 2003 | 15058195 |
| dna based classification of food associated enterobacteriaceae previously identified by biolog gn microplates. | enterobacteriaceae are frequently isolated from food products and it is essential to have methods for correct identification for both food hygiene and epidemiology reasons. phenotypic methods are not always sufficient and have to be supplemented by dna based methods. in the present study, 70 strains of enterobacteriaceae derived from milk, fish and meat that had previously been identified by biolog gn microplates were genomically classified together with 15 representative type strains of species ... | 2004 | 15046311 |
| histamine-related hygienic qualities and bacteria found in popular commercial scombroid fish fillets in taiwan. | to determine the histamine-related hygienic qualities and bacteria of scombroid fish fillets sold in traditional retail markets, 61 samples were collected from northern and southern taiwan. it was found that the content of volatile base nitrogen in most samples was below 25 mg/100 g, which is the regulatory level in taiwan. the ratio of unacceptable samples/total samples for aerobic plate count and escherichia coli was 100% and 15% in northern samples and 100% and 20% in southern samples, respec ... | 2004 | 14968980 |
| culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. | renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ... | 2003 | 14662931 |
| soil microbial populations, community composition, and activity as affected by repeated applications of hog and cattle manure in eastern saskatchewan. | a field site near humboldt, saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. during each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot and foliar diseases. microbial activity in soils treated with cattle manure was higher than in soils treated with hog manure or urea. similarly, nitrous oxide emissions from soil in ... | 2003 | 14608420 |
| exposure to airborne gram-negative bacteria, dust and endotoxin in paper factories. | air samples for determination of the concentration of gram-negative bacteria, dust and endotoxin were collected at 10 sites in 2 large pulp and paper mills (paper factories) located in northern poland, of which one (plant "a") was an older type facility while the other (plant "b") was a modern, fully automated factory with an effective ventilatory system. in both factories paper was produced from wood chips derived mostly from scots pine. the concentrations of gram-negative bacteria in the air o ... | 2003 | 12852739 |
| effects of bacterial treatments on wood extractives. | bacterial strains were isolated from spruce wood chips and their ability to reduce the content of wood extractives was studied. strains were screened by cultivation on liquid media containing wood extractives as the major nutrient. some bacterial species could decrease remarkably the amount of extractives in the liquid media and reduced the amount of triglycerides, steryl esters and total extractives by 100, 20 and 39%, respectively. spruce wood chips were treated in controlled conditions with s ... | 2003 | 12770505 |
| development of a plating medium for selection of helicobacter pylori from water samples. | the goal of this study was to develop a simple plating medium to allow large-scale screening of water samples for the presence of helicobacter pylori. five conventional plating media (brain heart infusion, brucella agar, columbia blood agar base, campylobacter agar kit skirrow, and hpspa medium), each containing a commercial antibiotic supplement, were initially evaluated. eight strains selected as common waterborne organisms (acinetobacter, aeromonas, bacillus, escherichia coli, enterobacter, e ... | 2003 | 12732566 |
| characterization of five phyllosphere bacteria isolated from rosa rugosa leaves, and their phenotypic and metabolic properties. | five gram-negative bacteria, all of which were enterobacteriaceae, were isolated from the phyllosphere of green or senescing leaves of rosa rugosa, and their phenotypic and physiological characteristics were examined. partial 16s rdna sequences led to identification of these isolates as pantoea agglomerans, klebsiella terrigena, erwinia rhapontici, and two strains of rahnella aquatilis. interestingly, these phyllosphere bacteria had certain phenotypic and physiological convergences, while they s ... | 2002 | 12506991 |
| identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina. | from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ... | 2002 | 12384342 |
| molecular characterization of the growth phase-dependent expression of the lsra gene, encoding levansucrase of rahnella aquatilis. | expression of the lsra gene from rahnella aquatilis, encoding levansucrase, is tightly regulated by the growth phase of the host cell; low-level expression was observed in the early phase of cell growth, but expression was significantly stimulated in the late phase. northern blot analysis revealed that regulation occurred at the level of transcription. the promoter region was identified by primer extension analysis. two opposite genetic elements that participate in the regulation of lsra express ... | 2002 | 12374819 |
| differences in attachment of salmonella enterica serovars and escherichia coli o157:h7 to alfalfa sprouts. | numerous salmonella enterica and escherichia coli o157:h7 outbreaks have been associated with contaminated sprouts. we examined how s. enterica serovars, e. coli serotypes, and nonpathogenic bacteria isolated from alfalfa sprouts grow on and adhere to alfalfa sprouts. growth on and adherence to sprouts were not significantly different among different serovars of s. enterica, but all s. enterica serovars grew on and adhered to alfalfa sprouts significantly better than e. coli o157:h7. e. coli o15 ... | 2002 | 12324317 |
| response of furniture factory workers to work-related airborne allergens. | the aim of this work was to determine the reactivity of furniture factory workers to microbial allergens associated with wood dust. allergological examinations by skin and precipitin tests were performed in 48 workers employed in a factory producing furniture from fibreboards and chipboards, and in 32 healthy urban dwellers not exposed to organic dusts (referents). the skin test was performed by the intradermal method with the saline extracts of the cultures of 3 microbial species (rahnella sp., ... | 2002 | 12088404 |
| formation of guaiacol in chocolate milk by the psychrotrophic bacterium rahnella aquatilis. | the aim of this study was to identify the causative agent of a smoky/phenolic taint in refrigerated full cream chocolate milk. | 2001 | 11696092 |
| biochemical-genetic characterization of the chromosomally encoded extended-spectrum class a beta-lactamase from rahnella aquatilis. | from whole-cell dna of a clinical isolate of the enterobacterial species rahnella aquatilis, a beta-lactamase gene was cloned that encoded a chromosomally encoded ambler class a enzyme, rahn-1. rahn-1, with a pi of 7.2, shares 76, 73, and 71% amino acid identity with the extended-spectrum beta-lactamase of chromosomal origin from serratia fonticola and with the plasmid-mediated beta-lactamases ctx-m-2 and ctx-m-1, respectively. the hydrolysis spectrum of the clavulanic acid-inhibited rahn-1 was ... | 2001 | 11557504 |
| identity and antibiotic susceptibility of enterobacterial flora of salad vegetables. | the enterobacterial flora from carrots (organic and non-organic) and salad vegetables has been identified and antibiotic susceptibilities determined. pseudomonas fluorescens and pantoea (formerly enterobacter) agglomerans were the species most commonly found; the former was usually resistant to at least six of the antibiotics under test. rahnella aquatilis (often producing beta-lactamase) was also found in carrots. there were no clear differences in flora from organic and non-organic carrots. th ... | 2001 | 11463532 |
| rahnella aquatilis bacteremia in a patient with relapsed acute lymphoblastic leukemia. | rahnella aquatilis infections are rare. we report the case of a 46-y-old african-american male with relapsed acute lymphoblastic leukemia who had r. aquatilis bacteremia after beginning reinduction chemotherapy. he was treated for 4 weeks with piperacillin-tazobactam and gentamicin. he recovered from the infection and had an allogenic bone marrow transplant a month later. | 2001 | 11450870 |
| response of sawmill workers to work-related airborne allergens. | the aim of this work was to determine the reactivity of sawmill workers to biological allergens associated with wood dust. allergological examinations by skin and precipitin tests were performed in 43 workers employed in a sawmill processing coniferous wood (pine), in 90 workers employed in two sawmills processing deciduous wood (oak), and in 32 healthy urban dwellers not exposed to organic dusts (referents). the skin test was performed by the intradermal method with the saline extracts of wood ... | 2001 | 11426929 |
| exposure to airborne microorganisms in polish sawmills. | microbiological air sampling was performed in four sawmills located in eastern poland, of which two were processing coniferous wood (pine, fir) and other two deciduous wood (oak, birch). total concentration of microorganisms (bacteria and fungi) in the air of sawmills processing coniferous wood was on average 20.2 +/- 5.6 x 10(3) cfu/m(3) (mean +/- s.e.) and significantly (p < 0.05) higher compared to those processing deciduous wood where the mean concentration of airborne microorganisms was 9.8 ... | 2001 | 11426928 |
| comparison of methods for determining the numbers and species distribution of coliform bacteria in well water samples. | enumeration of coliform bacteria and escherichia coli is the most widely used method in the estimation of hygienic quality of drinking water. the yield of target bacteria and the species composition of different populations of coliform bacteria may depend on the method.three methods were compared. | 2001 | 11412314 |
| identification by 16s ribosomal rna gene sequencing of an enterobacteriaceae species with ambiguous biochemical profile from a renal transplant recipient. | traditional ways of identification of bacteria by phenotypic characteristics cannot be used for non-cultivable organisms and organisms with unusual biochemical profiles. in this study, an enterobacteriaceae was isolated in pure growth from the mid-stream urine of a 67-year old renal transplant recipient with urinary tract infection. conventional biochemical tests did not reveal a pattern resembling any known member of the enterobacteriaceae family. the vitek system (gni+) showed that it was 18% ... | 2001 | 11248520 |
| role of yersinia intermedia and pseudomonas putida in the development of a fruity off-flavour in pasteurized milk. | analysis by gas chromatography-mass spectrometry of pasteurized milk with a fruity (pineapple like) off odour and a sour, rancid and soapy taste indicated the presence of concentrations at microg/ml levels of ethyl butanoate, ethyl hexanoate, ethyl octanoate, ethyl decanoate, octanoic acid, decanoic acid and dodecanoic acid. the off-odour and taste were attributed to the presence of these compounds in the milk. microbiological examination confirmed that the milk was also contaminated with a seri ... | 2000 | 11131069 |
| synthesis of methyl beta-d-fructoside catalyzed by levansucrase from rahnella aquatilis. | methyl beta-d-fructoside(mf) was formed from sucrose and methanol by a transfructosylation reaction using recombinant levansucrase from rahnella aquatilis. the increase in the yield of mf formation was achieved by increasing methanol concentration. the enzyme stability at higher concentrations of methanol was maintained by lowering the reaction temperature. the optimum temperature and sucrose concentration for mf formation was 10 degrees c and 50 gl(-1) respectively and the yield of mf was 70%. | 2000 | 11064046 |
| [isolation of rahnella aquatilis in the tracheostomy exudate from a patient with laryngeal cancer]. | 2000 | 10974779 | |
| molecular cloning of a gene encoding the thermoactive levansucrase from rrahnella aquatilis and its growth phase-dependent expression in eescherichia coli. | a levansucrase gene (lsra) from rahnella aquatilis atcc33071 was isolated from a genomic library and the nucleotide sequence of the lsra structural gene was determined. lsra is composed of 1248 bp and encodes 415 amino acid residues with a calculated molecular mass of 45.9 kda. although the amino acid sequence of lsra gene showed good conservation with the sequences of reported levansucrases and of the conserved regions thought to be implicated in the enzyme activity, comparison of the deduced a ... | 2000 | 10936661 |
| natural antibiotic susceptibility of rahnella aquatilis and r. aquatilis-related strains. | a database is described of the natural susceptibilities of 70 rahnella strains to 71 antibiotics. mic values were determined by a microdilution procedure and evaluated by a table calculation program. rahnella aquatilis and r. aquatilis-related strains were naturally resistant to amoxycillin, ticarcillin, fosfomycin and to antibiotics to which other species of enterobacteriaceae are also intrinsically resistant, i.e. macrolides (except azithromycin), benzylpenicillin, oxacillin, rifampicin, fusid ... | 2000 | 10768513 |