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quantification by qpcr of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level.currently, there is little evidence available on the development of predictive models for the diagnosis or prognosis of chronic periodontitis based on the qpcr quantification of subgingival pathobionts. our objectives were to: (1) analyze and internally validate pathobiont-based models that could be used to distinguish different periodontal conditions at site-specific level within the same patient with chronic periodontitis; (2) develop nomograms derived from predictive models. subgingival plaqu ...201728848499
microbiological response to periodontal therapy and multivariable prediction of clinical outcome.this study assessed the microbiological effects of a 2-phase antimicrobial periodontal therapy and tested microbiological, clinical, and biological markers as prognostic indicators for clinical success.201728844191
spondylodiscitis due to parvimonas micra diagnosed by the melting temperature mapping method: a case report.it has been suggested that more than 100 bacterial species can be identified using only seven universal bacterial primer sets in the melting temperature (tm) mapping method and that these findings can be obtained within 3 h of sterile site collection.201728835212
emerging role of actinomyces meyeri in brain abscesses: a case report and literature review.we describe a case of a 29-year-old man from pakistan who presented with progressive neurologic symptoms over 1 week and was found to have a right parietal cerebral abscess. neurosurgical drainage cultures showed growth of actinomyces meyeri, streptococcus intermedius, and parvimonas micra. an abscessed molar was identified as the likely port of entry and was extracted. the patient was treated with metronidazole, vancomycin, and doxycycline because of prior anaphylaxis to penicillin. at 6-month ...201728831384
mucosal microbiome dysbiosis in gastric carcinogenesis.we aimed to characterise the microbial changes associated with histological stages of gastric tumourigenesis.201728765474
oesophageal pleural fistula presenting with parvimonas micra infection causing cervical and brain abscesses.parvimonas micra (p. micra) infections causing spinal cord compression are extremely rare. we report an occult oesophageal pleural fistula presenting with spinal epidural and brain abscesses resulting in severe neurological deficits caused by p. micra. molecular detection proved to be instrumental in identifying the causative pathogen. essential management with decompression, drainage, antibiotics and fistula repair lead to a good outcome.201728648472
pleural effusion due to parvimonas micra. a case report and a literature review of 30 cases.the clinical and microbiological characteristics of infections caused by parvimonas micra is described, including 30 cases in the literature and a new case handled at the present centre. out of the 31 patients, 18 were male; mean age at diagnosis was 65.1 ± 13.0 years. infection site was the vertebral spine in 14 patients and joints and heart valves in 5 each one; pain was present in all patients with articular localization and in almost all patients with vertebral involvement. the diagnosis was ...201728537064
acute mono-arthritis of the knee: a case report of infection with parvimonas micra and concomitant pseudogout.parvimonas micra is a rare pathogen for septic arthritis and is known for its subacute onset. we report a case of acute arthritis of the knee caused by p. micra and pseudogout. initially, calcium pyrophosphate crystals were found in the knee, which were successfully treated with a steroid injection. only anaerobic cultures became positive. a 16s rrna pcr-analysis was necessary to identify p. micra as causative agent, a method which is never described before in similar cases. the infection was tr ...201628529856
herpes simplex 1 and periopathogen role in peri-implantitis.the objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type i (hsv-1) in implant surfaces between participants with peri-implantitis (pi) and healthy peri-implant tissues and to quantitatively assess the relation between hsv-1 and periopathogens inside the microbiological profile associated with pi.201728512280
effect of preparation method and storage period on the stability of saliva dna.saliva is an attractive source for oral microbial detection and quantification since sampling is non-invasive and rapid.201728460249
leveraging sequence-based faecal microbial community survey data to identify a composite biomarker for colorectal cancer.colorectal cancer (crc) is the second leading cause of cancer-associated mortality in the usa. the faecal microbiome may provide non-invasive biomarkers of crc and indicate transition in the adenoma-carcinoma sequence. re-analysing raw sequence and metadata from several studies uniformly, we sought to identify a composite and generalisable microbial marker for crc.201728341746
brain abscess potentially resulting from odontogenic focus: report of three cases and a literature review.odontogenic foci can rarely cause intracranial infection. hematogenous spread is considered to be the most important pathophysiological mechanism of intracranial infection of odontogenic origin. to investigate the oral origin of intracranial infections, oral surgeons should understand the underlying mechanisms by which oral bacteria spread to the central nervous system. however, there have been very few reports of intracranial infection resulting from odontogenic infection.201728286386
evaluation of bacteriological profile in the apical root segment of the patients with primary apical periodontitis.apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. the reason for this disparity might be the technique used for collecting the samples from the pulp canals. hence, we carried out the present study to evaluate the microbial flora in the apical part ...201728050984
the use of different irrigation techniques to decrease bacterial loads in healthy and diabetic patients with asymptomatic apical periodontitis.diabetes mellitus is a multisystem disease which weakens the human's immunity. subsequently, it worsens the sequelae of apical periodontitis by raising a fierce bacterial trait due to the impaired host response.201628028421
low antibiotic resistance among anaerobic gram-negative bacteria in periodontitis 5 years following metronidazole therapy.the objective of this study was to assess antibiotic susceptibility among predominant gram-negative anaerobic bacteria isolated from periodontitis patients who 5 years prior had been subject to mechanical therapy with or without adjunctive metronidazole. one pooled sample was taken from the 5 deepest sites of each of 161 patients that completed the 5 year follow-up after therapy. the samples were analyzed by culture. a total number of 85 anaerobic strains were isolated from the predominant subgi ...201727988390
photoinactivation using visible light plus water-filtered infrared-a (vis+wira) and chlorine e6 (ce6) eradicates planktonic periodontal pathogens and subgingival biofilms.alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. antimicrobial photodynamic therapy (apdt) has recently gained attention as a novel method to eradicate pathogens. the aim of this study was to evaluate the antimicrobial effects of a novel apdt method using visible light (vis) and water infiltrated infrared a (wira) in combination with chlorine e6 (ce6) against different periodontal pathogens in planktonic form and ...201627965635
bacteraemia due to parvimonas micra, a commensal pathogen, in a patient with an oesophageal tumour.a man aged 53 years was admitted to our hospital due to general malaise, fever and chills for the past 24 hours. he had a history of chronic alcoholic liver disease. the blood tests showed leucocytosis with neutrophilia, lactic acidosis and acute-phase reactants. the blood cultures were positive for parvimonas micra, an anaerobic pathogen which is part of the flora of the oral cavity. there was no evidence of abscess formation in either the examination or the imaging tests, but in the work-up th ...201627864301
quantitation of faecal fusobacterium improves faecal immunochemical test in detecting advanced colorectal neoplasia.there is a need for an improved biomarker for colorectal cancer (crc) and advanced adenoma. we evaluated faecal microbial markers for clinical use in detecting crc and advanced adenoma.201627797940
comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample?the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control).201727786617
effect of smokeless tobacco products on human oral bacteria growth and viability.to evaluate the toxicity of smokeless tobacco products (stps) on oral bacteria, seven smokeless tobacco aqueous extracts (staes) from major brands of stps and three tobacco-specific n-nitrosamines (tsnas) were used in a growth and viability test against 38 oral bacterial species or subspecies. all seven staes showed concentration-dependent effects on the growth and viability of tested oral bacteria under anaerobic culture conditions, although there were strain-to-strain variations. in the presen ...201627756619
real-time pcr analysis of fungal organisms and bacterial species at peri-implantitis sites.the potential role of fungal organisms and their co-aggregation with either periodontopathogens or opportunistic pathogens at peri-implantitis sites is unknown. the aim of the present study was to qualitatively/quantitatively analyze and correlate fungal organisms and bacterial species at peri-implantitis sites.201527747631
bacterial diversity of symptomatic primary endodontic infection by clonal analysis.the aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. samples were collected from 10 patients and submitted to bacterial dna isolation, 16s rrna gene amplification, cloning, and sequencing. a bacterial genomic library was constructed and bacterial diversity was estimated. the mean number of taxa per canal was 15, ranging from 11 to 21. a total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) w ...201627737357
does obesity influence the subgingival microbiota composition in periodontal health and disease?to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp).201627717180
effect of bioactive dental adhesive on periodontal and endodontic pathogens.the objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. nanoparticles of amorphous ca ...201627699574
herpesviral-bacterial co-infection in mandibular third molar pericoronitis.the aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis.201627620215
bacterial colonization of the peri-implant sulcus in dentate patients: a prospective observational study.the aim of the present study was to compare the composition of the periodontal microflora at baseline (t0) with the submucosal microflora at least 1 year after implant placement (t1) in periodontally healthy patients.201727558381
development of a cavity disinfectant containing antibacterial monomer mdpb.an experimental cavity disinfectant (acc) that is intended to be used for various direct and indirect restorations was prepared by adding an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (mdpb) at 5% into 80% ethanol. the antibacterial effectiveness of acc and its influences on the bonding abilities of resin cements were investigated. to examine the antibacterial activity of unpolymerized mdpb, the minimum inhibitory and bactericidal concentrations (mic and mbc) were determine ...201627558263
oral behavior, dental, periodontal and microbiological findings in patients undergoing hemodialysis and after kidney transplantation.aim of this single center cross-sectional study was to investigate oral behavior, dental, periodontal and microbiological findings in patients undergoing hemodialysis (hd) and after kidney transplantation (kt).201627531498
[intramedullary abscess of the cervical spinal cord caused by advanced periodontitis:case report].we describe the case of a 60-year-old man with an intramedullary abscess of the cervical spinal cord caused by advanced periodontitis. he suddenly developed severe neck pain and rapidly progressive palsy of the left upper arm. t2-weighted sagittal magnetic resonance imaging(mri)revealed a hyperintense area extending from c1 to c6. gadolinium-enhanced t1-weighted mri showed a ring-enhanced lesion at the c3-4 level that was hyperintense on diffusion-weighted mri. the patient underwent drainage of ...201627506846
assessment of the antibacterial, cytotoxic and mutagenic potential of the phenolic-rich hydroalcoholic extract from copaifera trapezifolia hayne leaves.copaifera trapezifolia hayne occurs in the atlantic rainforest, which is considered one of the most important and endangered tropical forests on the planet. although literature works have described many copaifera spp., their biological activities remain little known. in the present study, we aimed to evaluate (1) the potential of the hydroalcoholic extract from c. trapezifolia leaves (cte) to act against the causative agents of tooth decay and apical periodontitis and (2) the cytotoxicity and mu ...201627452156
cytokine and microbial profiles in relation to the clinical outcome following treatment of peri-implantitis.to study whether cytokine levels and bacterial counts in p atients with peri-implantitis reflect clinical treatment outcome following non-surgical management.201627422156
frequency of periodontal pathogens and helicobacter pylori in the mouths and stomachs of obese individuals submitted to bariatric surgery: a cross-sectional study.this cross-sectional study compared the frequency of oral periodontopathogens and h. pylori in the mouths and stomachs of obese individuals with or without periodontitis submitted to bariatric surgery.201627383704
molecular identification of cultivable bacteria from infected root canals associated with acute apical abscess.the objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16s rrna) of cultivable bacteria. two hundred and twenty strains isolated by culture from 20 root canals were subjected to dna extraction and amplification of the 16s rrna gene (pcr), followed by sequencing. the resulting nucleotide sequences were compared to the genbank database from the national center of biotechnology inform ...201727224567
the prevalence of oral flora in the biofilm microbiota of the endotracheal tube.the endotracheal tube (ett) is recognized as an independent factor for infection in intubated patients. the presence of biofilm contributes to the development of pneumonia. standard culturing techniques are inadequate to detect many of the bacteria present in a biofilm. delineation of the microbiota in the ett is needed to further understand infections in ventilated patients. a prospective, observational study was performed at a university, level i trauma center. twenty ett were collected at ext ...201627215719
microbial colonization of the peri-implant sulcus and implant connection of implants restored with cemented versus screw-retained superstructures: a cross-sectional study.the aim of this study is to investigate peri-implant and intraconnection microflora of healthy implants restored with cemented and screwed superstructures.201627153291
association of clinical parameters with periodontal bacterial haemolytic activity.to determine whether haemolytic activity of subgingival bacteria is associated with periodontitis clinical parameters and to identify which bacteria produce the haemolysins.201627105613
the predictive value of microbiological findings on teeth, internal and external implant portions in clinical decision making.the primary aim of this study was to evaluate 23 pathogens associated with peri-implantitis at inner part of implant connections, in peri-implant and periodontal pockets between patients suffering peri-implantitis and participants with healthy peri-implant tissues; the secondary aim was to estimate the predictive value of microbiological profile in patients wearing dental implants using data mining methods.201627079924
microbiota-based model improves the sensitivity of fecal immunochemical test for detecting colonic lesions.colorectal cancer (crc) is the second leading cause of death among cancers in the united states. although individuals diagnosed early have a greater than 90% chance of survival, more than one-third of individuals do not adhere to screening recommendations partly because the standard diagnostics, colonoscopy and sigmoidoscopy, are expensive and invasive. thus, there is a great need to improve the sensitivity of non-invasive tests to detect early stage cancers and adenomas. numerous studies have i ...201627056827
accuracy of commercial kits and published primer pairs for the detection of periodontopathogens.despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. the aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens.201627020914
aerococcus christensenii as part of severe polymicrobial chorioamnionitis in a pregnant woman.chorioamnionitis is a potentially life threatening infection of the fetal membranes, commonly caused by ascending bacteria from the vagina and cervix. in our case, a healthy nullipara with a term pregnancy presented clinical signs of infection after induced labour with an intracervical balloon. thick green and foul smelling amniotic fluid was observed and culture showed massive growth of aerococcus christensenii, a facultative anaerob species found in the human vagina, previously only rarely all ...201627014376
arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota.rheumatoid arthritis (ra) and periodontitis (pd) are chronic inflammatory disorders that cause bone loss. pd tends to be more prevalent and severe in ra patients. previous experimental studies demonstrated that ra triggers alveolar bone loss similarly to pd. the aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. checkerboard dna-dna hybridization was employed to analyze forty oral bacterial species in 3 groups of c ...201626996070
oral microbiota species in acute apical endodontic abscesses.acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions.201626983837
parvimonas micra: a rare cause of native joint septic arthritis.parvimonas micra is a fastidious, anaerobic, gram positive coccus, which is found in normal human oral and gastrointestinal flora. it has also been known as peptostreptococcus micros and micromonas micros with its most recent re-classification in 2006. it has been described in association with hematogenous seeding of prosthetic joints [1,2]. several cases of discitis and osteomyelitis have been described in association with dental procedures and periodontal disease often with a subacute presenta ...201626911898
evaluation of antibiotic susceptibility of gram-positive anaerobic cocci isolated from cancer patients of the n. n. blokhin russian cancer research center.in total, 81 nonduplicate gram-positive anaerobic cocci (gpac) were involved in this study. the gpac were isolated from samples collected from cancer patients between 2004 and 2014. species identification was carried out by matrix-assisted laser desorption ionization time of flight mass spectrometry (maldi-tof ms). the majority of isolates were identified as finegoldia magna (47%) and peptoniphilus harei (28%). the susceptibility of six species of gpac was determined for eight antibiotics accord ...201526798518
in-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis.the aim of the present study was to assess the antimicrobial activity of a sodium hypochlorite formulation including its components against bacteria associated with periodontal disease.201626759339
n-substituted n'-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)guanidines--antibacterial, cytotoxic activities and some structure-activity relationships.a series of n-substituted n'-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)guanidine derivatives bearing sulfonamide moiety have been screened in vitro for antibacterial activity against isolates from patients with infections of oral cavity, respiratory tract and intestinal tract. the majority of compounds exhibited good antibacterial potency. 1-[4-chloro-5-methyl-2-(4-trifluoromethylbenzylthio)benzenesulfonyl]-3-(3-sulfamoylphenyl)guanidine (13) showed very strong activity, with mic ≤ 6.2 μg/ml ...201526638540
microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation.this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp).201526521147
first case of infectious endocarditis caused by parvimonas micra.p. micra is an anaerobic gram-positive cocci, and a known commensal organism of the human oral cavity and gastrointestinal tract. although it has been classically described in association with endodontic disease and peritonsillar infection, recent reports have highlighted the role of p. micra as the primary pathogen in the setting of invasive infections. in its most recent taxonomic classification, p. micra has never been reported causing infectious endocarditis in humans. here, we describe a 71 ...201526485192
two cases of spondylodiscitis caused by parvimonas micra. 201526429222
metagenomic analysis of faecal microbiome as a tool towards targeted non-invasive biomarkers for colorectal cancer.to evaluate the potential for diagnosing colorectal cancer (crc) from faecal metagenomes.201726408641
in vitro culture of previously uncultured oral bacterial phylotypes.around a third of oral bacteria cannot be grown using conventional bacteriological culture media. community profiling targeting 16s rrna and shotgun metagenomics methods have proved valuable in revealing the complexity of the oral bacterial community. studies investigating the role of oral bacteria in health and disease require phenotypic characterizations that are possible only with live cultures. the aim of this study was to develop novel culture media and use an in vitro biofilm model to cult ...201526407883
delayed infection with parvimonas micra following spinal instrumentation.delayed-onset surgical site infections following spinal instrumentation are uncommon and often present with chronic pain and implant failure. anaerobic organisms are rarely implicated and identified with difficulty in these infections.201526359730
qat chewing and periodontal pathogens in health and disease: further evidence for a prebiotic-like effect.qat chewing has been reported to induce subgingival microbial shifts suggestive of prebiotic-like properties. the objective here was to assess the effect of qat chewing on a panel of classical and new putative periopathogens in health and periodontitis.201526351631
spinal infections caused by parvimonas micra. 201526344818
oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients.the objective of this study was to identify the dna of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight (pb/lbw) neonates.201526259070
microbiologic and clinical findings of implants in healthy condition and with peri-implantitis.to compare implants in healthy conditions and implants with peri-implantitis with regard to their clinical parameters and the microbiologic composition at the peri-implant sulcus, inside the implant connection, and the gingival sulcus of neighboring teeth.201626252036
detection of subgingival periodontal pathogens--comparison of two sampling strategies.the aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant.201626193958
first confirmed case of spondylodiscitis with epidural abscess caused by parvimonas micra.parvimonas micra was renamed species as within gram-positive anaerobic cocci and rarely causes severe infections in healthy people. we report the first confirmed case of spondylodiscitis with epidural abscess caused by p. micra in a healthy women. the patient has a pain in low back and anterior left thigh. magnetic resonance imaging and computed tomography detected the affected lesion at the l2 and l3 vertebral bodies. all isolates from the surgical and needle biopsy specimens were identified as ...201526188420
advanced caries microbiota in teeth with irreversible pulpitis.bacterial taxa in the forefront of caries biofilms are candidate pathogens for irreversible pulpitis and are possibly the first ones to invade the pulp and initiate endodontic infection. this study examined the microbiota of the most advanced layers of dentinal caries in teeth with irreversible pulpitis.201526187422
tlr4, nod1 and nod2 mediate immune recognition of putative newly identified periodontal pathogens.periodontitis is a polymicrobial inflammatory disease that results from the interaction between the oral microbiota and the host immunity. although the innate immune response is important for disease initiation and progression, the innate immune receptors that recognize both classical and putative periodontal pathogens that elicit an immune response have not been elucidated. by using the human oral microbe identification microarray (homim), we identified multiple predominant oral bacterial speci ...201626177212
periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased dominican adults.the objective of this study was to compare the periodontopathogen prevalence and tetracycline resistance genes in dominican patients with different periodontal conditions.201526121972
lemierre's syndrome: an unusual presentation. 201526117663
spondylodiscitis due to anaerobic bacteria about a case of parvimonas micra infection.parvimonas micra is a rare isolate in clinical specimens. we report a case of spondylodiscitis caused by p. micra, a rarely reported gram positive cocci. the case was an elderly patient with joint surgery and ischaemic heart disease history. infection resolved after adequate antibiotic therapy.201526067839
antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants.phosphoric acid has been suggested as an irrigant due to its effectiveness in removing the smear layer.201526018307
bacteremic meningitis caused by parvimonas micra in an immunocompetent host.a 61-year-old man with chronic hepatitis b and dyslipidemia visited the emergency department with a fever and severe headache. he was diagnosed with bacterial meningitis after a lumbar puncture, and blood culture revealed parvimonas micra bacteremia. although he had a history of extraction of a molar two weeks before symptom onset, there was no evidence of abscess formation on physical examination or imaging studies. he was successfully treated with oral metronidazole for 12 days after 9 days of ...201525977161
morbidly obese patient with non-alcoholic steatohepatitis-related cirrhosis who died from sepsis caused by dental infection of porphyromonas gingivalis: a case report.non-alcoholic steatohepatitis (nash) is associated with increased risks of developing lifestyle-related diseases including type 2 diabetes, cardiovascular disease and cerebral vessel disease. while the two-hit hypothesis and, recently, multiple parallel hits hypothesis of nash pathogenesis were proposed, further details have not emerged. recently, dental infection of porphyromonas gingivalis (p. gingivalis) has been reported as a critical risk factor for nash progression, which acts as multiple ...201625943712
quantitation of biofilm and planktonic life forms of coexisting periodontal species.complexity of oral polymicrobial communities has prompted a need for developing in vitro models to study behavior of coexisting bacteria. little knowledge is available of in vitro co-growth of several periodontitis-associated species without early colonizers of dental plaque.201525926392
microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective study.to determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface.201625827437
short-chain fatty acids in infected root canals of teeth with apical periodontitis before and after treatment.short-chain fatty acids (scfas) are bacterial metabolic end products that may function as virulence factors. this study evaluated the occurrence of scfas in infected root canals before and after treatment.201525799538
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents - special references to bacteria isolated between april 2011 and march 2012].bacteria isolated from surgical infections during the period from april 2011 to march 2012 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 785 strains including 31 strains of candida spp. were isolated from 204 (78.8%) of 259 patients with surgical infections. five hundred and twenty three strains were isolated from primary infections, and 231 strains were isolated from surgical site infection. from primary infections, anaerobic gram-ne ...201425796741
subgingival microbial communities in leukocyte adhesion deficiency and their relationship with local immunopathology.leukocyte adhesion deficiency i (lad-i) is a primary immunodeficiency caused by single gene mutations in the cd18 subunit of β2 integrins which result in defective transmigration of neutrophils into the tissues. affected patients suffer from recurrent life threatening infections and severe oral disease (periodontitis). microbial communities in the local environment (subgingival plaque) are thought to be the triggers for inflammatory periodontitis, yet little is known regarding the microbial comm ...201525741691
microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles.nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ...201525581046
effects of adjunctive daily phototherapy on chronic periodontitis: a randomized single-blind controlled trial.the purpose of this randomized single-blind controlled trial was to elucidate the clinical and antimicrobial effects of daily phototherapy (pt) as an adjunct to scaling and root planing (srp) in patients with chronic periodontitis.201425568808
combinations of bacterial species associated with symptomatic endodontic infections in a chinese population.to use microarrays to detect 11 selected bacteria in infected root canals, revealing bacterial combinations that are associated with clinical symptoms and signs of primary endodontic infections in a chinese population.201625565631
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2010 and march 2011].bacteria isolated from surgical infections during the period from april 2010 to march 2011 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 631 strains including 25 strains of candida spp. were isolated from 170 (81.7%) of 208 patients with surgical infections. four hundred and twenty two strains were isolated from primary infections, and 184 strains were isolated from surgical site infection. from primary infections, anaerobic gram-nega ...201425549405
microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients.the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ...201525536224
oral bacterial dna findings in pericardial fluid.we recently reported that large amounts of oral bacterial dna can be found in thrombus aspirates of myocardial infarction patients. some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing and a few with pcr; in purulent pericarditis, nevertheless, bacterial pcr has not been used as a diagnostic method before.201425412607
parvimonas micra chest wall abscess following transthoracic lung needle biopsy. 201425378986
antimicrobial activity of preparation bioaron c.the antimicrobial activity of sirupus bioaron c, a preparation, whose main ingredient is an extract from the leaves of aloe arborescens, was tested against different microorganisms isolated from patients with upper respiratory tract infections. the experiments were performed on 40 strains: 20 strains of anaerobic bacteria, 13 strains of aerobic bacteria and 7 strains of yeast-like fungi from the genus candida and on 18 reference strains (atcc). the antimicrobial activity of bioaron c (mbc and mf ...201425362808
[study of association between parvimonas micra and pulp dominant pathogens in the infected root canals with chronic periradicular periodontitis].to study the prevalence of parvimonas micra (pm) and the associations between pm and pulp dominant pathogens in order to reflect the colonization of pm in the infected root canals with chronic periradicular periodontitis.201425351224
subgingival bacterial recolonization after scaling and root planing in smokers with chronic periodontitis.the aim of this study was to compare subgingival bacterial recolonization patterns after scaling and root planing in current smokers and non-smokers.201525283721
hydrogen sulfide production from subgingival plaque samples.periodontitis is a polymicrobial anaerobe infection. little is known about the dysbiotic microbiota and the role of bacterial metabolites in the disease process. it is suggested that the production of certain waste products in the proteolytic metabolism may work as markers for disease severity. hydrogen sulfide (h2s) is a gas produced by degradation of proteins in the subgingival pocket. it is highly toxic and believed to have pro-inflammatory properties. we aimed to study h2s production from su ...201525280920
macrophage cell activation with acute apical abscess contents determined by interleukin-1 beta and tumor necrosis factor alpha production.this clinical study has investigated the antigenic activity of bacterial contents from exudates of acute apical abscesses (aaas) and their paired root canal contents regarding the stimulation capacity by levels of interleukin (il)-1 beta and tumor necrosis factor alpha (tnf-α) throughout the root canal treatment against macrophage cells.201425205261
the presence of cariogenic and periodontal pathogens in the oral cavity of one-year-old infants delivered pre-term with very low birthweights: a case control study.recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. however, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. the aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavi ...201425178742
empyema due to aggregatibacter aphrophilus and parvimonas micra coinfection. 201525138795
axenic culture of a candidate division tm7 bacterium from the human oral cavity and biofilm interactions with other oral bacteria.the diversity of bacterial species in the human oral cavity is well recognized, but a high proportion of them are presently uncultivable. candidate division tm7 bacteria are almost always detected in metagenomic studies but have not yet been cultivated. in this paper, we identified candidate division tm7 bacterial phylotypes in mature plaque samples from around orthodontic bonds in subjects undergoing orthodontic treatment. successive rounds of enrichment in laboratory media led to the isolation ...201425107981
high-level antimicrobial efficacy of representative mediterranean natural plant extracts against oral microorganisms.nature is an unexplored reservoir of novel phytopharmaceuticals. since biofilm-related oral diseases often correlate with antibiotic resistance, plant-derived antimicrobial agents could enhance existing treatment options. therefore, the rationale of the present report was to examine the antimicrobial impact of mediterranean natural extracts on oral microorganisms. five different extracts from olea europaea, mastic gum, and inula viscosa were tested against ten bacteria and one candida albicans s ...201425054150
quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case-control study.a number of species/phylotypes have been newly implicated as putative periopathogens. the objective of this study was to explore associations among classical and new pathogens in subgingival biofilm and to assess their relative importance to chronic periodontitis.201525040261
pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils.endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. to investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections (endodontic pathogens [ep]), i.e., fusobacterium nucleatum, streptococcus intermedius, parvimonas micra, and prevotella intermedia, was inoculated into subcutaneousl ...201425024367
microbial diversity in persistent root canal infections investigated by checkerboard dna-dna hybridization.the aim of the present study was to investigate the composition of the root canal microbiota in endodontic failures in order to identify and quantify these microorganisms.201424935532
trends in the susceptibility of commonly encountered clinically significant anaerobes and susceptibilities of blood isolates of anaerobes to 16 antimicrobial agents, including fidaxomicin and rifaximin, 2008-2012, northern taiwan.we investigated the antimicrobial resistance trends and profiles of clinical anaerobic isolates in northern taiwan. trends in the susceptibility of five commonly encountered clinical anaerobic isolates to seven agents from 2008 to 2012 were measured using the cochran-armitage trend test. the minimum inhibitory concentrations (mics) of 16 antimicrobial agents, including fidaxomicin and rifaximin, against anaerobic blood isolates from two medical centers were determined using the agar dilution met ...201424930042
antimicrobial susceptibility of clinical isolates of anaerobic bacteria in ontario, 2010-2011.the local epidemiology of antimicrobial susceptibility patterns in anaerobic bacteria is important in guiding the empiric treatment of infections. however, susceptibility data are very limited on anaerobic organisms, particularly among non-bacteroides organisms. to determine susceptibility profiles of clinically-significant anaerobic bacteria in ontario canada, anaerobic isolates from sterile sites submitted to public health ontario laboratory (phol) for identification and susceptibility testing ...201424923267
microbial evaluation of traumatized teeth treated with triple antibiotic paste or calcium hydroxide with 2% chlorhexidine gel in pulp revascularization.revascularization outcome depends on microbial elimination because apical repair will not happen in the presence of infected tissues. this study evaluated the microbial composition of traumatized immature teeth and assessed their reduction during different stages of the revascularization procedures performed with 2 intracanal medicaments.201424862703
clinical and microbiological characterization of localized aggressive periodontitis: a cohort study.localized aggressive periodontitis (lagp) is an infectious periodontal disease which generally affects young people. recent data suggest the involvement of different bacterial species in different populations. the causative bacterial species in israel has never been identified despite a high prevalence of lagp in this population. the objectives of this study were to characterize the bacterial microbiota of periodontal pockets within an israeli lagp population who were also clinically assessed.201424861390
hiding behind confusion: pleural empyema caused by parvimonas micra. 201424796229
effects of systemic administration of sitafloxacin on subgingival microflora and antimicrobial susceptibility profile in acute periodontal lesions.the aim of this study was to assess the effect(s) of systemic administration of sitafloxacin on subgingival microbial profiles of acute periodontal lesions. antimicrobial susceptibility of clinical isolates was also investigated. patients with acute phases of chronic periodontitis were subjected to clinical examination and microbiological assessment of their subgingival plaque samples by culture technique. sitafloxacin was then administered (100 mg/day for 5 days) systemically. the clinical and ...201424747615
evaluation of the bactericidal activity on oral organisms of the nd-yap laser in vitro.the bactericidal activity of a pulsed fibre-optic nd-yap laser on various strains of oral bacteria: prevotella nigrescens, streptococcus sanguis, actinomyces viscosus (i), fusobacterium nucleatum, actinobacillus actinomycetemcomitans and peptostreptococcus micros (i) was evaluated. a 200-μm optical fibre was placed in eppendorf tubes containing the different bacterial suspensions. at an energy of 170 mj/30 hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). the expo ...199824710990
presence of oral bacterial species in primary endodontic infections of primary teeth.knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions.201324683780
parvimonas micra as a causative organism of spondylodiscitis: a report of two cases and a literature review.spondylodiscitis caused by parvimonas micra, a rarely reported infection, might be under-detected using conventional methods. this report of the detection and treatment of two cases of spondylodiscitis due to p. micra and review of the literature indicates that the use of gene sequencing methods might improve the accuracy of diagnosing this infection.201424680818
oral cavity anaerobic pathogens in biofilm formation on voice prostheses.a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms.201524677258
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