Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the use of elisas for monitoring exposure of pig herds to brachyspira hyodysenteriae. | abstract: background: swine dysentery (sd), a mucohaemorrhagic diarrhoeal disease of pigs, results from infection of the large intestine with the spirochaete brachyspira hyodysenteriae. elisa systems using whole spirochaete cells (wc) and the b. hyodysenteriae outer membrane lipoprotein bhlp29.7 previously have been established as potential diagnostic tools for sd. however, their true value in identifying infected herds remains unclear. the present study aimed to compare the performance of whol ... | 2012 | 22248341 |
| Analysis of bacterial load and prevalence of mixed infections with Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli in German pigs with diarrhoea. | Lawsonia (L.) intracellularis, Brachyspira (B.) hyodysenteriae and B. pilosicoli are important pathogens in domestic pig production world-wide, responsible for porcine intestinal adenomatosis, swine dysentery, and porcine intestinal spirochetosis, respectively. Conventional PCR is the major diagnostic tool in the detection of the three pathogens, but the sole detection of bacterial DNA might lead to misinterpretations of results with respect to their clinical relevance, especially with mixed inf ... | 2011 | 22059295 |
| Demonstration of genes encoding virulence and virulence life-style factors in Brachyspira spp. isolates from pigs. | The distribution of many genes encoding virulence and virulence life-style (VL-S) factors in Brachyspira (B.) hyodysenteriae and other Brachyspira species are largely unknown. Their knowledge is essential e.g. for the improvement of diagnostic methods targeting the detection and differentiation of the species. Thus 121 German Brachyspira field isolates from diarrhoeic pigs were characterized down to the species level by restriction fragment length polymorphism analysis of the nox gene and subseq ... | 2011 | 22047713 |
| phenotypic and genetic diversity among intestinal spirochaetes (genus brachyspira) in free-living wild mallards (anas platyrhynchos) sampled in southern sweden. | brachyspira spp. are anaerobic intestinal spirochaetes that colonize vertebrates. some species cause enteric diseases in pigs, chickens and possibly in humans, whereas others display a commensual relationship with their hosts. the aims were to investigate the prevalence among colonized free-living wild mallards (anas platyrhynchos) of three enteropathogenic brachyspira spp., and to describe the biodiversity of brachyspira spp. isolates. isolates from 150 birds were screened by pcr for 3 pathogen ... | 2011 | 22036527 |
| a 14-year-old with rectal bleeding and abdominal pain. | 2011 | 21970991 | |
| evaluation of the use of recombinant bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with brachyspira hyodysenteriae. | aims:ôçé aim of the study is to evaluate the use of recombinant bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with brachyspira hyodysenteriae. methods and results:ôçé sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (sd) were examined. sera from 378 sows from farms with previous sd history were examined via immunoblotting. specific antib ... | 2011 | 21838749 |
| bloodstream infection due to brachyspira pilosicoli in a patient with multiorgan failure. | brachyspira pilosicoli is an etiological agent of human intestinal spirochetosis. bloodstream infection due to this microorganism is rare. we report a case of b. pilosicoli bacteremia in a 70-year-old patient who presented with multiorgan failure. | 2011 | 21832021 |
| complete genome sequence of brachyspira intermedia reveals unique genomic features in brachyspira species and phage-mediated horizontal gene transfer. | abstract: | 2011 | 21816042 |
| antimicrobial susceptibility of brachyspira spp. isolated from commercial laying hens and free-living wild mallards (anas platyrhynchos). | in vitro antimicrobial susceptibility to tylosin, valnemulin, tiamulin, doxycycline, lincomycin and ampicillin was investigated by broth dilution in 48 brachyspira spp. isolates from commercial laying hens (n=30) and free-living wild mallards (anas platyrhynchos) (n=18). presumed pathogens (brachyspira alvinipulli, brachyspira intermedia, brachyspira pilosicoli), commensals (brachyspira murdochii, brachyspira innocens, "brachyspira pulli"), and isolates of undetermined species affiliation were i ... | 2011 | 21812718 |
| a high dietary concentration of inulin is necessary to reduce the incidence of swine dysentery in pigs experimentally challenged with brachyspira hyodysenteriae. | a total of sixty surgically castrated male pigs (large white-á+ù-álandrace) weighing 31-à2 (sd 4-à3)-ákg were used in a randomised block experiment to examine the effect of added dietary inulin (0, 20, 40 and 80-ág/kg) on the occurrence of swine dysentery (sd) and on fermentation characteristics in the large intestine after experimental challenge with the causative spirochaete brachyspira hyodysenteriae. the pigs were allowed to adapt to the diets for 2 weeks before each pig was challenged orall ... | 2011 | 21736788 |
| telling the whole story in a 10,000-genome world. | abstract: | 2011 | 21714939 |
| lactobacilli antagonize the growth, motility, and adherence of brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis. | avian intestinal spirochetosis (ais) results from the colonization of the ceca and colorectum of poultry by pathogenic brachyspira species. the number of cases of ais has increased since the 2006 european union ban on the use of antibiotic growth promoters, which, together with emerging antimicrobial resistance in brachyspira, has driven renewed interest in alternative intervention strategies. probiotics have been reported as protecting livestock against infection with common enteric pathogens, ... | 2011 | 21666022 |
| experimental inoculation of day-old ducks with brachyspira pilosicoli and b. alvinipulli. | two groups of one-day-old peking ducklings (groups i and ii, 12 birds/group) were inoculated orally with brachyspira pilosicoli and two groups with b. alvinipulli (groups iii and iv, 12 birds/group). t-2 toxin was added to the feed of groups ii and iv in a dose of 1 mg/kg of feed. groups v and vi served as uninfected control groups (ducks of group vi received t-2 toxin). the body weight gain of the ducks was measured and clinical signs were monitored continuously. the birds were sacrificed and n ... | 2011 | 21665570 |
| antimicrobial susceptibility pattern of brachyspira intermedia isolates from european layers. | a broth microdilution method was used to determine the antimicrobial susceptibility of 20 brachyspira intermedia isolates obtained from different layer flocks in belgium and the netherlands between 2008 and 2010. the antimicrobial agents used were tylosin, tilmicosin, tiamulin, valnemulin, doxycycline, and lincomycin. the minimal inhibitory concentration (mic) distribution patterns of tylosin, tilmicosin, lincomycin, and doxycycline were bimodal, demonstrating acquired resistance against doxycyc ... | 2011 | 21663534 |
| trends towards lower antimicrobial susceptibility and characterization of acquired resistance among clinical isolates of brachyspira hyodysenteriae in spain. | antimicrobial susceptibility of clinical isolates of brachyspira hyodysenteriae in spain was monitored and the underlying molecular mechanisms of resistance were investigated. minimal inhibitory concentrations of tylosin, tiamulin, valnemulin, lincomycin and tylvalosin were determined for 87 b. hyodysenteriae isolates recovered from 2008 to 2009 by broth dilution. domain v of the 23s rrna gene and the ribosomal protein l3 gene were sequenced in 20 isolates with tiamulin mic ≥4 μg/ml, presenting ... | 2011 | 21555771 |
| the use of quantitative pcr for identification and quantification of brachyspira pilosicoli, lawsonia intracellularis and escherichia coli fimbrial types f4 and f18 in pig feces. | four quantitative pcr (qpcr) assays were evaluated for quantitative detection of brachyspira pilosicoli, lawsonia intracellularis, and e. coli fimbrial types f4 and f18 in pig feces. standard curves were based on feces spiked with the respective reference strains. the detection limits from the spiking experiments were 10(2) bacteria/g feces for bpilo-qpcr and laws-qpcr, 10(3)cfu/g feces for f4-qpcr and f18-qpcr. the pcr efficiency for all four qpcr assays was between 0.91 and 1.01 with r(2) abov ... | 2011 | 21530108 |
| typing of brachyspira spp. from rodents, pigs and chickens on swedish farms. | the aim of the current study was to look for evidence of possible cross-species transmission of brachyspira species between rodents and farm animals. to do this, previously collected and characterised brachyspira isolates from rodents, pigs and chickens on the same farms were analysed by random amplified polymorphic dna (rapd). isolates with similar rapd banding patterns were further typed by pulsed-field gel electrophoresis (pfge). identical isolates of brachyspira pilosicoli, brachyspira inter ... | 2011 | 21497461 |
| characterisation of multiresistant brachyspira hyodysenteriae isolates from czech pig farms. | the aim of the present study was to evaluate the importance of clonal spread of brachyspira hyodysenteriae resistant to pleuromutilins (tiamulin, valnemulin) on farms in the czech republic. agar dilution method and macrorestriction fragment profile analysis by pulsed field gel electrophoresis were used to characterise 35 b hyodysenteriae isolates that were obtained from clinical cases of swine dysentery on 32 farms between 2000 and 2005. most isolates showed multiple resistances to tiamulin, val ... | 2011 | 21493555 |
| evidence that the 36kb plasmid of brachyspira hyodysenteriae contributes to virulence. | swine dysentery (sd) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete brachyspira hyodysenteriae. recently the genome of virulent australian b. hyodysenteriae strain wa1 was sequenced, and a 36kilobase (kb) circular plasmid was identified. the plasmid contained 31 genes including six rfb genes that were predicted to be involved with rhamnose biosynthesis, and others associated with glycosylation. in the current study a set of pcrs was developed to a ... | 2011 | 21458173 |
| typhlocolitis associated with spirochaetes in duck flocks. | the aetiology of increased mortality observed in two breeder duck flocks (flock a consisting of 3500 laying ducks and flock b comprising 4300 laying ducks) during the first egg-laying season was studied. in flocks a and b, 773 ducks and 715 ducks (18.4% and 16.6%) died within a 24-week and a 20-week period, respectively. death was preceded by clinical signs including movement difficulties, lack of appetite and depression lasting for 1 to 2 days. diarrhoea was not observed. on gross pathological ... | 2011 | 21331945 |
| faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis. | this study aimed to obtain information about the types of spirochaetes colonising urban dogs in thailand, and to investigate their pathogenic potential in a day-old chick model of intestinal spirochaetosis. spirochaetes were isolated from the faeces of six of 47 (12.8%) healthy dogs and 11 of 104 (10.6%) dogs with diarrhoea. their biochemical properties and 16s ribosomal dna sequences were analysed. four isolates were identified as brachyspira pilosicoli, three resembled "brachyspira pulli", nin ... | 2011 | 21320714 |
| identification of a new steroid degrading bacterial strain h5 from the baltic sea and isolation of two estradiol inducible genes. | the presence of steroid hormones in the aquatic environment is potentially threatening the population dynamics of all kinds of sea animals and public health. environmental estrogens in water have been reported to be associated with abnormal sexual development and abnormal feminizing responses in some animals. new approaches for the bioremediation of steroid hormones from the environment are therefore urgently sought. we have previously isolated a steroid degrading bacterial strain (h5) from the ... | 2011 | 21310233 |
| complete genome sequence of brachyspira murdochii type strain (56-150). | brachyspira murdochii stanton et al. 1992 is a non-pathogenic, host-associated spirochete of the family brachyspiraceae. initially isolated from the intestinal content of a healthy swine, the 'group b spirochaetes' were first described as serpulina murdochii. members of the family brachyspiraceae are of great phylogenetic interest because of the extremely isolated location of this family within the phylum 'spirochaetes'. here we describe the features of this organism, together with the complete ... | 2010 | 21304710 |
| enterohepatic helicobacter species | most of the members of the genus helicobacter do not normally colonize the gastric mucosa, but instead thrive in the mucosal surfaces of the intestinal tract and/or the liver of humans, other mammals, and birds. these enterohepatic helicobacter species have features of ultrastructure and physiology in common with helicobacter pylori and the other gastric helicobacter species, and have been the subject of several recent ... | 2001 | 21290746 |
| transmission of swine pathogens: different means, different needs. | there seems to be two main types of pathogens that cause diseases in swine: those that are mainly introduced through direct pig contacts, and those that are often, and in some situations mainly introduced by indirect transmission means. in this review, the mange mite (sarcoptes scabiei), toxigenic pasteurella multocida and brachyspira hyodysenteriae will be used as examples of the first type, and foot and mouth disease virus, mycoplasma hyopneumoniae and porcine reproductive and respiratory synd ... | 2011 | 21241537 |
| investigations on the prevalence and potential pathogenicity of intestinal trichomonads in pigs using in situ hybridization. | in pigs, three different trichomonad species (tritrichomonas foetus, tetratrichomonas buttreyi and tritrichomonas rotunda) have been described as commensals in the large intestine. the aim of this study was to gain further knowledge on the prevalence and pathogenicity of trichomonads in pigs by using a morphology-based approach. chromogenic in situ hybridization (ish) is a technique which allows direct localization of the protozoa in the intestinal tissue and correlation of the infection with pa ... | 2010 | 21236578 |
| [swine dysentery eradication in a grower-finisher farm in switzerland]. | on a swiss grower-finisher farm blood-tinged-diarrhoea in pigs weighing 40 to 60 kg was observed during several months, resulting in reduced feed efficiency and a prolonged fattening period. as part of a research project, in february 2007 faecal samples were analysed and one diseased pig was euthanised and sent for necropsy where typical gut lesions indicative for a brachyspira (b.) hyodysenteriae infection were found. b. hyodysenteriae was demonstrated by pcr in 4 out of 5 faecal samples. the p ... | 2011 | 21210368 |
| observations of variable inter-observer agreement for clinical evaluation of faecal consistency in grow-finishing pigs. | inter-observer agreement for assessment of faecal consistency in pigs was evaluated using a scoring system with 3 categories. in a pilot study, 3 observers performed an examination of faecal samples post-collection. the samples were obtained from pigs (12-13 weeks old) in 4 herds with a history of diarrhoea associated with lawsonia intracellularis, brachyspira spp. and/or porcine circovirus type 2. observer 1 examined all the faecal samples from the 4 herds. observer 2 only examined the faecal s ... | 2010 | 21183234 |
| allelic variations of the nox gene of brachyspira pilosicoli impair its detection by qpcr. | 2010 | 21129860 | |
| exposure to norepinephrine enhances brachyspira pilosicoli growth, attraction to mucin and attachment to caco-2 cells. | brachyspira pilosicoli is an anaerobic intestinal spirochaete that colonizes the large intestine of a variety of species of birds and mammals, including human beings. colonization may result in a mild colitis and diarrhoea in a condition known as 'intestinal spirochaetosis'. the catecholamine norepinephrine (ne), which is known to influence the behaviour of many bacterial species, may be present in the colon. the purpose of the current study was to determine whether exposure of b. pilosicoli to ... | 2010 | 21051488 |
| the major surface vsp proteins of brachyspira hyodysenteriae form antigenic protein complexes. | the vsp proteins are the major outer membrane proteins of brachyspira hyodysenteriae, the causative agent of swine dysentery. eight vsp genes have been identified in b. hyodysenteriae strain b204, arranged into two four-gene loci, and at least two of the corresponding proteins are produced in vitro. the aims of this study were to characterise the vsp genes of the virulent australian b. hyodysenteriae strain x576 and their corresponding proteins, genomic sequence comparison with strains b204 and ... | 2010 | 21036493 |
| in vitro antimicrobial susceptibility of brachyspira hyodysenteriae strains isolated in japan from 1985 to 2009. | the antimicrobial susceptibilities of 72 brachyspira hyodysenteriae isolates collected from clinical cases of swine dysentery (sd) in 11 prefectures in japan between 1985 and 2009 were investigated by an agar dilution method using five antimicrobial agents. there is a tendency of japanese field isolates of b. hyodysenteriae to acquire resistance to the main antimicrobials used in sd treatment such as tiamulin, valnemulin, and efrotomycin. a responsible approach for selection and use of antimicro ... | 2010 | 20697185 |
| the complete genome sequence of the pathogenic intestinal spirochete brachyspira pilosicoli and comparison with other brachyspira genomes. | the anaerobic spirochete brachyspira pilosicoli colonizes the large intestine of various species of birds and mammals, including humans. it causes "intestinal spirochetosis", a condition characterized by mild colitis, diarrhea and reduced growth. this study aimed to sequence and analyse the bacterial genome to investigate the genetic basis of its specialized ecology and virulence. | 2010 | 20625514 |
| characterization of autonomic nerve markers and lymphocyte subsets in the ileal peyer's patch of pigs infected experimentally with brachyspira hyodysenteriae. | the aim of the present study was to investigate potential interrelationships between immune and neural elements of peyer's patches in normal pigs (n=8) and in pigs infected experimentally with brachyspira hyodysenteriae and suffering from swine dysentery (n=8). assessment of tissue concentration of neuropeptides by enzyme linked immunosorbent assay revealed increased levels of galanin (gal) and substance p (sp) in samples from the infected animals. in contrast, concentrations of vasoactive intes ... | 2010 | 20605161 |
| prevalence of brachyspira pilosicoli and "brachyspira canis" in dogs and their association with diarrhoea. | the aims of this study were to investigate the prevalence of colonization with intestinal spirochaetes in dogs, and to assess their association with diarrhoea. to achieve this, faecal samples from 311 dogs were obtained between november 2008 and april 2009 and cultured for brachyspira species. a total of 41 brachyspira spp. isolates were recovered, and these were classified into species according to their biochemical properties, and results of a b. pilosicoli species-specific pcr, and partial am ... | 2010 | 20570060 |
| multiple-locus variable-number tandem-repeat analysis of the swine dysentery pathogen, brachyspira hyodysenteriae. | the spirochete brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe colonic infection of pigs that has a considerable economic impact in many swine-producing countries. in spite of its importance, knowledge about the global epidemiology and population structure of b. hyodysenteriae is limited. progress in this area has been hampered by the lack of a low-cost, portable, and discriminatory method for strain typing. the aim of the current study was to develop and test a mu ... | 2010 | 20554811 |
| diets containing inulin but not lupins help to prevent swine dysentery in experimentally challenged pigs. | swine dysentery is a contagious mucohemorrhagic diarrheal disease caused by the intestinal spirochete brachyspira hyodysenteriae that colonizes and induces inflammation of the cecum and colon. it has been reported that a diet containing chicory root and sweet lupin can prevent swine dysentery. this experiment was conducted to test the hypothesis that inulin in the chicory root rather than galactans in lupins was responsible for protective effects. an experiment with a 2 × 2 factorial arrangement ... | 2010 | 20525927 |
| a multiplex real-time pcr for the simultaneous detection and quantitation of brachyspira hyodysenteriae, brachyspira pilosicoli and lawsonia intracellularis in pig faeces. | a multiplex real-time pcr assay was developed to detect and quantify b. hyodysenteriae, b. pilosicoli, and l. intracellularis in pig faeces. specific probes and primers were directed against the nadh oxidase (nox) gene of brachyspira and the aspartate ammonia lyase (aspa) gene of l. intracellularis, respectively. the analytical sensitivity for the real-time pcr assay, expressed as limit of detection (lod) was below 10 dna copies for l. intracellularis, 14 dna copies for b. pilosicoli and 26 dna ... | 2010 | 20496826 |
| prophylactic treatment with hypoxis hemerocallidea corm (african potato) methanolic extract ameliorates brachyspira hyodysenteriae-induced murine typhlocolitis. | brachyspira hyodysenteriae is the causative agent of swine dysentery and induces a characteristic mucosal inflammation resulting in pronounced typhlocolitis in swine and mice. hypoxis hemerocallidea corm (african potato) is a traditional medicine in southern africa. an african potato methanolic extract (apme) and one of its major constituents, hypoxoside, have been shown in vitro to possess an anti-inflammatory property. the aim of this study is to evaluate the ability of apme to prevent or amel ... | 2010 | 20404038 |
| human intestinal spirochaetosis in northern japan. | a histological diagnosis of human intestinal spirochaetosis (his) was made in 114 patients during the period 1994-2007. all patients lived in three prefectures in the northern part of honshu, japan. most patients were elderly and male. twenty-nine patients complained of abdominal pain, bloody stools, diarrhoea or bowel symptoms, but most patients showed no direct symptoms of bowel disease, and occult faecal blood detected at medical check-up was the main reason for colonoscopic examination. ther ... | 2010 | 20378723 |
| differential regulation of the multiple flagellins in spirochetes. | the expression of flagellin genes in most bacteria is typically regulated by the flagellum-specific sigma(28) factor flia, and an anti-sigma(28) factor, flgm. however, the regulatory hierarchy in several bacteria that have multiple flagellins is more complex. in these bacteria, the flagellin genes are often transcribed by at least two different sigma factors. the flagellar filament in spirochetes consists of one to three flab core proteins and at least one flaa sheath protein. here, the genetica ... | 2010 | 20304988 |
| in vitro antagonistic activities of animal intestinal strains against swine-associated pathogens. | a wide range of enteropathogens cause costly diarrhoeal diseases in fattening piglets and account for food-related infections in humans. the objective of this study was to screen beneficial bacterial strains from the gastrointestinal tract of various animal sources for antagonistic activity against diverse pathogens associated with hazardous pig production times. using agar spot assays, 15 well-characterized strains belonging to lactobacillus, enterococcus, bifidobacterium and bacillus were stud ... | 2010 | 20226602 |
| human intestinal spirochetosis--a review. | human intestinal spirochetosis (is) is a condition defined histologically by the presence of spirochetal microorganisms attached to the apical cell membrane of the colorectal epithelium. intestinal spirochetes comprise a heterogeneous group of bacteria. in humans, brachyspira aalborgi and brachyspira pilosicoli predominate. prevalence rates of is are low where living standards are high, in contrast to poorly developed areas where is is common. homosexuals and hiv-infected individuals are at high ... | 2010 | 20200654 |
| brachyspira murdochii colitis in pigs. | the weakly beta-hemolytic porcine spirochete brachyspira murdochii is considered a normal intestinal commensal. in the present study, however, a field case of b murdochii-associated catarrhal colitis was identified in a pig, as characterized by extensive spirochetal colonization of the surface epithelium. experimentally, 8 weaned pigs were challenged with the b murdochii isolate, reproducing catarrhal colitis in 2 animals. by applying fluorescent in situ hybridization using a species-specific ol ... | 2010 | 20173181 |
| brachyspira intermedia and brachyspira pilosicoli are commonly found in older laying flocks in pennsylvania. | anaerobic intestinal spirochetes (genus brachyspira) include several species that are recognized as pathogens of poultry. surveys undertaken in europe and australia have shown that layer and breeder flocks are often colonized by the pathogenic species brachyspira intermedia and brachyspira pilosicoli, but similar surveys have not been conducted in the united states. in the current study, dna was extracted from fecal samples (n=50) collected from each of 21 flocks of laying hens >40 wk of age in ... | 2009 | 20095153 |
| the intestinal spirochete brachyspira pilosicoli attaches to cultured caco-2 cells and induces pathological changes. | brachyspira pilosicoli is an anaerobic spirochete that has received relatively little study, partly due to its specialized culture requirements and slow growth. this bacterium colonizes the large intestine of various species, including humans; typically, a dense layer of spirochete cells may be found intimately attached by one cell end to the surface of colonic enterocytes. colonized individuals may develop colitis, but the mechanisms involved are not understood. the current study aimed to devel ... | 2009 | 20020053 |
| evaluation of recombinant brachyspira pilosicoli oligopeptide-binding proteins as vaccine candidates in a mouse model of intestinal spirochaetosis. | the anaerobic intestinal spirochaete brachyspira pilosicoli colonizes the large intestine of humans, and various species of animals and birds, in which it may induce a mild colitis and diarrhoea. the aim of the current study was to evaluate the use of putative oligopeptide-binding proteins of b. pilosicoli as vaccine components. a partial genome sequence of b. pilosicoli porcine strain 95/1000 was subjected to bioinformatics analysis, and six genes predicted to encode oligopeptide-binding protei ... | 2010 | 19959628 |
| brachyspira intermedia strain diversity and relationships to the other indole-positive brachyspira species. | the aims of this study were to use multilocus sequence typing (mlst) to (i) investigate the population structure, diversity and molecular epidemiology of the weakly haemolytic anaerobic intestinal spirochaete brachyspira intermedia, and (ii) determine the relationship of the species to the other two indole-positive but strongly haemolytic brachyspira species--b. hyodysenteriae and "b. suanatina". seventy-seven b. intermedia isolates from pigs and chickens were analysed, with the nucleotide seque ... | 2010 | 19944544 |
| intestinal spirochetosis as a cause of chronic diarrhoea in patients with hiv infection: case report and review of the literature. | we describe a 77-year-old patient with hiv infection suffering from chronic diarrhoea whose colonoscopy findings showed normal appearance mucosa and tissue samples revealed the presence of a dense layer of spirochetes attached to the apical cell membrane. a literature search from 1996 to april 2009 identified 19 additional cases of intestinal spirochetosis in patients with hiv infection. analysis of cases showed that intestinal spirochetosis causes chronic diarrhoea in men who have sex with men ... | 2009 | 19843615 |
| imprint cytology detects floating brachyspira in human intestinal spirochetosis. | human intestinal spirochetosis is a colorectal infectious disease caused by 2 brachyspira species. its diagnosis is established by histology, culture, and polymerase chain reaction, but the value of cytologic examination in routine practice remains unclear. in this study, imprint cytology of biopsy specimens was examined for cytologic features specific to human intestinal spirochetosis. specimens were obtained from 65 colorectal regions (1-3 regions from each case) in 25 ultrastructurally and/or ... | 2010 | 19836054 |
| the activity and compatibility of the antibiotic tiamulin with other drugs in poultry medicine--a review. | tiamulin hydrogen fumarate is a semisynthetic derivative of the diterpene antibiotic pleuromutilin used in poultry medicine to treat mainly mycoplasma- and brachyspira-related diseases. its use over 30 yr has not generally increased the development of resistance to these pathogens but occasionally resistant isolates are encountered. tiamulin administered at therapeutic levels is relatively quickly absorbed, metabolized in the liver, and eliminated from the body of the bird after a withdrawal per ... | 2009 | 19834086 |
| attraction of brachyspira pilosicoli to mucin. | the anaerobic intestinal spirochaete brachyspira pilosicoli colonizes the large intestine of various species, including humans. in the colon this spirochaete can penetrate the overlying mucus layer, attach by one cell end to the underlying enterocytes, and initiate localized colitis and diarrhoea. the aim of this study was to investigate whether, as part of the colonization process, b. pilosicoli is attracted to mucin. fifteen b. pilosicoli strains isolated from humans, pigs, chickens and dogs, ... | 2010 | 19833772 |
| brachyspira species blood stream infection. | the spirochetes inhabiting the large intestines of humans and animals consist of a diverse group of related organisms. intestinal spirochetosis caused by brachyspira pilosicoli is a newly recognized enteric disease of human being and animals manifest as mild colitis and diarrhoea. few cases have been reported of spirochetaemia especially in critically ill and immunocomprised patients. this is another case of spirochetaemia in a patient diagnosed as a case of chronic liver disease secondary to he ... | 2009 | 19813693 |
| identification and antimicrobial susceptibility of porcine bacteria that inhibit the growth of brachyspira hyodysenteriae in vitro. | to identify bacilli, lactic acid bacteria and bifidobacteria that inhibit the growth of brachyspira hyodysenteriae. | 2010 | 19778354 |
| human intestinal spirochetosis accompanied by human immunodeficiency virus infection: a case report. | we present a middle-aged, heterosexual japanese man with mixed infections including human intestinal spirochetosis, which led us to the detection of human immunodeficiency virus (hiv) infection. the patient had syphilis without related physical or neurological findings. an examination for the serum antibody for hiv performed 9 years previously was negative. in a complete medical checkup at the present time, human intestinal spirochetosis and unspecified entamebic cysts were suggested by histolog ... | 2009 | 19727207 |
| experimental challenge of mallards (anas platyrhynchos) with brachyspira hyodysenteriae and "brachyspira suanatina" isolated from pigs and mallards. | brachyspira hyodysenteriae, the aetiological agent of swine dysentery, and a recently proposed and closely related enteropathogenic spirochaete "brachyspira suanatina", originally isolated from pigs or mallards (anas platyrhynchos), were used to inoculate week-old mallard ducklings orally or cloacally. the colonization rate, clinical outcome, faecal dry matter content, blood leucocyte counts and gross, microscopical and electron microscopical features 14-16 days post-inoculation were investigate ... | 2009 | 19640549 |
| characterization and epidemiological relationships of spanish brachyspira hyodysenteriae field isolates. | this research aimed to describe the genetic and phenotypic diversity of 74 spanish brachyspira hyodysenteriae field isolates, to establish epidemiological relationships between the isolates and to confirm the presence of tiamulin-resistant isolates in spain. for these purposes, we performed biochemical tests in combination with diagnostic pcr analysis for the identification of brachyspira spp. and for detection of the smpa/smpb gene. we also used antimicrobial susceptibility tests, random amplif ... | 2010 | 19480724 |
| spirochaetes as intestinal pathogens: lessons from a brachyspira genome. | anaerobic spirochaetes of the genus brachyspira have long been known as important gut pathogens of pigs, but increasingly they are recognised as causing disease in birds and other animal species, including human beings. the genome sequence of the major swine pathogen brachyspira hyodysenteriae was recently published, and this revealed extensive genome optimisation that leads to adaptation to the complex environment of the colon. the genome sequences of other pathogenic and non-pathogenic brachys ... | 2009 | 19405984 |
| mucosal gene expression profiles following the colonization of immunocompetent defined-flora c3h mice with helicobacter bilis: a prelude to typhlocolitis. | an aberrant immune response to the commensal microbiota is widely hypothesized to contribute to the development of inflammatory bowel disease. helicobacter bilis colonization of defined-flora mice has been shown to trigger host immune responses to the commensal flora. however, the magnitude of the effects on mucosal homeostasis following colonization with h. bilis has not been determined. using microarray analysis, differential gene expression within the cecal mucosa was assessed at 15, 30, or 4 ... | 2009 | 19397886 |
| the selection of single-chain fv antibody fragments specific to bhlp 29.7 protein of brachyspira hyodysenteriae. | single-chain antibodies (scfv) specific to brachyspira hyodysenteriae were isolated from a phagemid library. recombinant bhlp 29.7 protein was used for scfv selection and individual clones were tested by elisa and immunofluorescent test; four unique clones were isolated. one of selected clones was able to bind specifically b. hyodysenteriae in elisa and immunofluorescence test. this is the first report of species-specific recombinant antibodies against b. hyodysenteriae. | 2009 | 19381477 |
| multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of brachyspira hyodysenteriae. | the purpose of this study was to develop and apply a multilocus sequence typing (mlst) scheme to study the molecular epidemiology of brachyspira hyodysenteriae, the aetiological agent of swine dysentery. sequences of seven conserved genomic loci were examined in 111 b. hyodysenteriae strains. fifty-eight of these previously had been analysed by multilocus enzyme electrophoresis (mlee), and for some the results of pulsed field gel electrophoresis (pfge), restriction endonuclease analysis (rea) an ... | 2009 | 19369014 |
| in vitro antagonistic activities of lactobacillus spp. against brachyspira hyodysenteriae and brachyspira pilosicoli. | the sensitivity of brachyspira hyodysenteriae and brachyspira pilosicoli, respectively the causative agents of swine dysentery and porcine intestinal spirochaetosis to two probiotic lactobacillus strains, l. rhamnosus cncm-i-3698 and l. farciminis cncm-i-3699 was studied through viability, motility and coaggregation assays. the cell-free supernatant of these lactobacilli contains lactic acid, that is stressful for brachyspira (leading to the formation of spherical bodies), and lethal. it was dem ... | 2009 | 19356863 |
| application of real time pcr for diagnosis of swine dysentery. | evaluation of a genetic diagnostic technique using real time pcr of swine dysentery (sd) was performed using nox primers. culture, ordinary pcr and real time pcr were compared in this experiment. sixty-seven specimens from pigs with clinical signs of sd brought to a slaughterhouse in shibaura, tokyo, were used. b. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary pcr in 49 of the pigs and was detected by real time pcr in 54 of the pigs. furthermore, we were able to determ ... | 2009 | 19346708 |
| high prevalence of spirochetosis in cholera patients, bangladesh. | the microbes that accompany the etiologic agent of cholera, vibrio cholerae, are only now being defined. in this study, spirochetes from the genus brachyspira were identified at high titers in more than one third of cholera patients in bangladesh. spirochetosis should now be tracked in the setting of cholera outbreaks. | 2009 | 19331734 |
| image of the month. intestinal spirochetosis: an unusual cause of asymptomatic colonic ulceration. | 2010 | 19306942 | |
| rapid and accurate diagnosis of human intestinal spirochetosis by fluorescence in situ hybridization. | human intestinal spirochetosis (his) is associated with overgrowth of the large intestine by spirochetes of the genus brachyspira. the microbiological diagnosis of his is hampered by the fastidious nature and slow growth of brachyspira spp. in clinical practice, his is diagnosed histopathologically, and a significant portion of cases may be missed. fluorescence in situ hybridization (fish) is a molecular method that allows the visualization and identification of single bacteria within tissue sec ... | 2009 | 19279178 |
| genome sequence of the pathogenic intestinal spirochete brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine. | brachyspira hyodysenteriae is an anaerobic intestinal spirochete that colonizes the large intestine of pigs and causes swine dysentery, a disease of significant economic importance. the genome sequence of b. hyodysenteriae strain wa1 was determined, making it the first representative of the genus brachyspira to be sequenced, and the seventeenth spirochete genome to be reported. the genome consisted of a circular 3,000,694 base pair (bp) chromosome, and a 35,940 bp circular plasmid that has not p ... | 2009 | 19262690 |
| [long evolution of diarrhea with a debatable infectious etiology in a homosexual patient infected with hiv]. | 2009 | 19218004 | |
| a reverse vaccinology approach to swine dysentery vaccine development. | swine dysentery (sd) is a mucohaemorrhagic colitis of pigs resulting from infection of the large intestine with the anaerobic intestinal spirochaete brachyspira hyodysenteriae. whole-cell bacterin vaccines are available to help control sd, but their performance has been inconsistent. this study aimed to use a reverse vaccinology approach to identify b. hyodysenteriae proteins for use as recombinant vaccine components. nineteen open reading frames (orfs) predicted to encode potential vaccine cand ... | 2009 | 19179021 |
| development of a multiplex qpcr for detection and quantitation of pathogenic intestinal spirochaetes in the faeces of pigs and chickens. | anaerobic intestinal spirochaetes of the genus brachyspira include several important pathogenic species, particularly those infecting pigs and chickens. in this study a multiplex-quantitative polymerase chain reaction (m-qpcr) assay was developed based on amplification of a 198 base pair portion of the nadh oxidase gene, using taqman probes for detecting and quantifying the three main pathogenic species, b. hyodysenteriae, b. pilosicoli and b. intermedia. the specificity of the assay was validat ... | 2009 | 19171443 |
| single 23s rrna mutations at the ribosomal peptidyl transferase centre confer resistance to valnemulin and other antibiotics in mycobacterium smegmatis by perturbation of the drug binding pocket. | tiamulin and valnemulin target the peptidyl transferase centre (ptc) on the bacterial ribosome. they are used in veterinary medicine to treat infections caused by a variety of bacterial pathogens, including the intestinal spirochetes brachyspira spp. mutations in ribosomal protein l3 and 23s rrna have previously been associated with tiamulin resistance in brachyspira spp. isolates, but as multiple mutations were isolated together, the roles of the individual mutations are unclear. in this work, ... | 2009 | 19154331 |
| isolation of the anaerobic intestinal spirochaete brachyspira pilosicoli from long-term residents and indonesian visitors to perth, western australia. | brachyspira pilosicoli is an anaerobic spirochaete that colonizes the large intestine of humans and various species of animals and birds. the spirochaete is an important enteric pathogen of pigs and poultry, but its pathogenic potential in humans is less clear. in the current study, the occurrence of b. pilosicoli in faecal samples from 766 individuals in two different population groups in perth, western australia, was investigated by selective anaerobic culture. of 586 individuals who were long ... | 2009 | 19141744 |
| the histological features of intestinal spirochetosis in a series of 113 patients. | a multicenter retrospective review of cases diagnosed as spirochetosis was performed to determine the presenting symptoms and histological changes. a total of 113 cases were retrieved from the archives, consisting of 97 colorectal specimens and 16 appendices. in only 25 cases was the presenting symptom recorded as diarrhea. of the colorectal specimens, 87 (90%) showed no mucosal abnormality (apart from the spirochetes); the other 10 showed mucosal inflammation but 6 of them had a diagnosis of an ... | 2010 | 19117973 |
| refractory diarrhea in a patient with hiv infection. | 2009 | 19113983 | |
| identification of a divided genome for vsh-1, the prophage-like gene transfer agent of brachyspira hyodysenteriae. | the brachyspira hyodysenteriae b204 genome sequence revealed three vsh-1 tail genes, hvp31, hvp60, and hvp37, in a 3.6-kb cluster. the location and transcription direction of these genes relative to those of the previously described vsh-1 16.3-kb gene operon indicate that the gene transfer agent vsh-1 has a noncontiguous, divided genome. | 2009 | 19103931 |
| tiamulin activity against brachyspira hyodysenteriae. | 2008 | 19103625 | |
| nutritional influences on some major enteric bacterial diseases of pig. | there are several enteric bacterial diseases and conditions of pigs that require control to prevent overt disease, to reduce morbidity and mortality, and to improve the efficiency of production. traditionally, veterinarians, feed manufacturers and producers have relied upon antibiotics and minerals (for example, zno, cuso4) in diets for a large part of this control. however, recent trends, particularly in europe, are to reduce antimicrobial use and seek alternative or replacement strategies for ... | 2002 | 19087411 |
| antimicrobial susceptibility testing of spanish field isolates of brachyspira hyodysenteriae. | this study is the first conducted in spain to evaluate antimicrobial susceptibility of field isolates of brachyspira hyodysenteriae. one hundred and eight isolates of the bacterium, recovered from different spanish swine farms between 2000 and 2007, were investigated. the minimum inhibitory concentrations (mic) of erythromycin, tylosin, tiamulin, valnemulin, clindamycin and lincomycin were determined using a broth microdilution technique. most of the isolates showed poor susceptibility to erythr ... | 2009 | 19084246 |
| in vitro susceptibility of bovine digital dermatitis associated spirochaetes to antimicrobial agents. | bovine digital dermatitis (bdd) is an infectious lameness in cattle, which has a large global impact in terms of animal welfare and cost. the majority of evidence suggests that spirochaetes are the aetiological agent of this disease. the aim of this study was to identify the susceptibility of bdd associated spirochaetes to a range of antimicrobial agents with a view to potential usage in vivo to treat this widespread cattle disease. a microdilution method was adapted to determine the in vitro su ... | 2009 | 19081208 |
| tiamulin activity against brachyspira hyodysenteriae. | 2008 | 19060324 | |
| intestinal spirochetes isolated from wild-living jackdaws, hooded crows and rooks (genus corvus): provisionally designated "brachyspira corvi" sp. nov. | intestinal spirochetes of genus brachyspira are commonly isolated from mammalian and avian hosts, and several species have been reported to cause enteric disease in pigs and birds. except for a previous publication on three isolates from corvid birds (order passeriformes, family corvidae, genus corvus), of which two are further studied in this paper, no other reports exist on brachyspira spp. of passerine birds. in this study, cloacal and intestinal swabs of small and large intestines were colle ... | 2008 | 18952187 |
| identification of genes associated with prophage-like gene transfer agents in the pathogenic intestinal spirochaetes brachyspira hyodysenteriae, brachyspira pilosicoli and brachyspira intermedia. | vsh-1 is an unusual prophage-like gene transfer agent (gta) that has been described in the intestinal spirochaete brachyspira hyodysenteriae. the gta does not self-propagate, but it assembles into a virus-like particle and transfers random 7.5kb fragments of host dna to other b. hyodysenteriae cells. to date the gta vsh-1 has only been analysed in b. hyodysenteriae strain b204, in which 11 late function genes encoding prophage capsid, tail and lysis elements have been described. the aim of the c ... | 2009 | 18950961 |
| infections with weakly haemolytic brachyspira species in pigs with miscellaneous chronic diseases. | the prevalence of infections with different brachyspira species was assessed in 202 pigs with various chronic herd problems using different methods. twenty-seven pigs (13.4%) were positive for brachyspira spp. with at least one of the methods used. the highest number of positives was identified with mucosal scraping-pcr (23), followed by pet-pcr (22) and bacteriological-biochemical analysis (15). with the exception of three cases of b. pilosicoli infections, only weakly pathogenic brachyspira sp ... | 2009 | 18823725 |
| detection of brachyspira hyodysenteriae, lawsonia intracellularis and brachyspira pilosicoli in feral pigs. | feral pigs are recognized as being a potential reservoir of pathogenic microorganisms that can infect domestic pigs and other species. the aim of this study was to investigate whether feral pigs in western australia were colonized by the pathogenic enteric bacteria lawsonia intracellularis, brachyspira hyodysenteriae and/or brachyspira pilosicoli. a total of 222 feral pigs from three study-populations were sampled. dna was extracted from faeces or colonic contents and subjected to a previously d ... | 2009 | 18818030 |
| brachyspira pilosicoli bloodstream infections: case report and review of the literature. | brachyspira pilosicoli is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. here, we describe the case of a b. pilosicoli spirochetemia in a 53-year-old male patient suffering from cardiogenic shock. this fastidious bacterium was isolated from blood, likely after translocation from the intestinal tract. blood cultures were positive after 5 days of incubation (one day after the patient's death), highlighting the problem of the reco ... | 2008 | 18817558 |
| an unexpectedly high prevalence of colonization with the intestinal spirochaete brachyspira aalborgi amongst residents of the indonesian island of bali. | pcr assays designed to amplify dna from the anaerobic intestinal spirochaete brachyspira aalborgi were conducted on dna extracted from 938 faecal samples from 469 residents on the indonesian island of bali. the individuals tested were sampled twice in one year and were from four rural villages, one peri-urban centre and the capital city, denpasar. overall, an unexpectedly high prevalence of colonization (24.7%) was found, with prevalence rates at different locations varying from a low of 15.6% a ... | 2008 | 18809551 |
| identification of weakly beta-hemolytic porcine spirochetes by biochemical reactions, pcr-based restriction fragment length polymorphism analysis and species-specific pcr. | we examined the usefulness of pcr-based restriction fragment length polymorphism (pcr-rflp) and species-specific pcr combined with a newly devised rapid biochemical test using microplates for identifying weakly beta-hemolytic intestinal spirochetes (wbhis) isolated from pigs. wbhis strains showing atypical biochemical characteristics were decisively identified at the species level by pcr-rflp and species-specific pcr. identification of wbhis at the species level in routine diagnostic work will c ... | 2008 | 18772561 |
| [multiresistant brachyspira hyodysenteriae in a dutch sow herd]. | this case study describes the isolation ofa multiresistant strain ofbrachyspira hyodysenteriae in april 2007 in a dutch sow herd with recurrent diarrhoea. examination of faecal samples taken from 7-month-old breeding gilts with diarrhoea revealed the presence of resistance against tiamulin, lincomycin, tylosin, doxycycline, and tylvalosin (the active substance in aivlosin) in four of five samples. tiamulin resistance has not been reported in the netherlands before. the repeated use of tiamulin o ... | 2008 | 18767301 |
| vaccination with an autogenous bacterin fails to prevent colonization by brachyspira intermedia in experimentally infected laying chickens. | avian intestinal spirochaetosis (ais) is a disease complex affecting adult laying and breeding chickens associated with infection by anaerobic intestinal spirochaetes of the genus brachyspira. options for control of ais are limited, as few effective antimicrobial agents are registered for use in laying chickens. one of the two most commonly encountered pathogenic species in ais is b. intermedia, and the aim of the current study was to determine whether a b. intermedia bacterin vaccine would help ... | 2009 | 18760550 |
| penicillin resistance in the intestinal spirochaete brachyspira pilosicoli associated with oxa-136 and oxa-137, two new variants of the class d beta-lactamase oxa-63. | penicillin resistance mediated by beta-lactamase activity has been reported previously in the anaerobic intestinal spirochaete brachyspira pilosicoli, and a novel class d beta-lactamase (oxa-63) hydrolysing oxacillin was described recently in a resistant human strain from france. in the current study, 18 b. pilosicoli strains from australia and papua new guinea were tested for ampicillin and oxacillin susceptibility, and investigated for the presence of the class d beta-lactamase gene blaoxa-63 ... | 2008 | 18719182 |
| blood concentrations of the cytokines il-1beta, il-6, il-10, tnf-alpha and ifn-gamma during experimentally induced swine dysentery. | knowledge of the cytokine response at infection with brachyspira hyodysenteriae can help understanding disease mechanism involved during swine dysentery. since this knowledge is still limited the aim of the present study was to induce dysentery experimentally in pigs and to monitor the development of important immunoregulatory cytokines in blood collected at various stages of the disease. | 2008 | 18700003 |
| evaluation of recombinant bhlp29.7 as an elisa antigen for detecting pig herds with swine dysentery. | swine dysentery (sd) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete brachyspira hyodysenteriae. diagnosis of sd traditionally has relied on detecting the spirochaete in the faeces of acutely affected pigs. to date simple and reliable serological assays that can be applied as a diagnostic tool at the herd level have not been available. in the current study a recombinant histidine tagged 29.7 kda lipoprotein of b. hyodysenteriae (his6-bhlp29.7) was ... | 2009 | 18619744 |
| prevalence, disease associations and risk factors for colonization with intestinal spirochaetes (brachyspira spp.) in flocks of laying hens in north-eastern italy. | the present study investigated the occurrence of anaerobic intestinal spirochaetes of the genus brachyspira in laying hen flocks in treviso province, north-eastern italy, with respect to prevalence, spirochaete species present, disease associations and risk factors for colonization. a total of 450 faecal samples from 45 sheds on 29 laying hen farms were cultured for intestinal spirochaetes. nineteen sheds on 12 farms contained chickens with symptoms consistent with avian intestinal spirochaetosi ... | 2008 | 18568654 |
| genetic analysis of spirochete flagellin proteins and their involvement in motility, filament assembly, and flagellar morphology. | the filaments of spirochete periplasmic flagella (pfs) have a unique structure and protein composition. in most spirochetes, the pfs consist of a core of at least three related proteins (flab1, flab2, and flab3) and a sheath of flaa protein. the functions of these filament proteins remain unknown. in this study, we used a multidisciplinary approach to examine the role of these proteins in determining the composition, shape, and stiffness of the pfs and how these proteins impact motility by using ... | 2008 | 18556797 |
| functional analysis of the campylobacter jejuni cj0183 and cj0588 genes. | the cj0183 and cj0588 genes identified in the campylobacter jejuni nctc 11168 genome encode proteins with amino acid sequences predicted to be homologous to other bacterial hemolysins. the cj0183 protein exhibits homology to brachyspira hyodysenteriae tlyc protein, whereas the cj0588 gene product is homologous to tlya proteins brachyspira hyodysenteriae, helicobacter pylori, and mycobacterium tuberculosis, which play a crucial role in bacterial virulence. the aim of our work was to examine the h ... | 2008 | 18389311 |
| phenotypic and molecular characterization of brachyspira spp. isolated from laying hens in different housing systems. | several species of intestinal spirochaetes, brachyspira (b.) alvinipulli, b. intermedia and b. pilosicoli, may cause reduced egg production and faecal staining of eggshells in chickens. the aim of this study was to characterize potentially pathogenic and presumably non-pathogenic brachyspira spp. from commercial laying hens. selective culture, phenotyping, pcr and 16s rrna gene sequencing were used and clinical data were collected. phenotypic profiles were obtained for 489 isolates and 351 isola ... | 2008 | 18375077 |
| collateral effects of antibiotics: carbadox and metronidazole induce vsh-1 and facilitate gene transfer among brachyspira hyodysenteriae strains. | brachyspira hyodysenteriae is an anaerobic spirochete and the etiologic agent of swine dysentery. the genome of this spirochete contains a mitomycin c-inducible, prophage-like gene transfer agent designated vsh-1. vsh-1 particles package random 7.5-kb fragments of the b. hyodysenteriae genome and transfer genes between b. hyodysenteriae cells. the chemicals and conditions inducing vsh-1 production are largely unknown. antibiotics used in swine management and stressors inducing traditional propha ... | 2008 | 18359835 |
| development and validation of an enzyme-linked immunosorbent assay for the diagnosis of porcine proliferative enteropathy. | the objective of this study was to develop an indirect enzyme-linked immunosorbent assay (elisa) using a sonicated pure culture of lawsonia intracellularis as the antigen (so-elisa). a total of 332 serum samples, consisting of 232 experimentally infected animals and 100 animals naturally infected with l. intracellularis, were used to assess the diagnostic sensitivity. three hundred and fifty-five sera from uninfected animals were used to determine the diagnostic specificity. the receiver operati ... | 2008 | 18319429 |
| genetic and biochemical characterization of oxa-63, a new class d beta-lactamase from brachyspira pilosicoli bm4442. | brachyspira pilosicoli bm4442, isolated from the feces of a patient with diarrhea at the hospital saint-michel in paris, was resistant to oxacillin (mic > 256 microg/ml) but remained susceptible to cephalosporins and to the combination of amoxicillin and clavulanic acid. cloning and sequencing of the corresponding resistance determinant revealed a coding sequence of 807 bp encoding a new class d beta-lactamase named oxa-63. the bla oxa-63 gene was chromosomally located and not part of a transpos ... | 2008 | 18212108 |
| identification and genetic fingerprinting of brachyspira species. | six brachyspira type and reference strains, and 14 well characterized porcine field isolates representing all recognised porcine brachyspira spp. were compared by different molecular methods. sequence analysis of the 16s rrna and the nox genes, pulsed-field gel electrophoresis (pfge) and randomly amplified polymorphic dna (rapd) were used in the study. in addition the isolates were analysed by five species-specific pcr systems. the topologies of the dendrograms obtained from each of the four typ ... | 2008 | 18096259 |