Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| the role for the small cryptic plasmids as moldable vectors for genetic innovation in aeromonas salmonicida subsp. salmonicida. | in aeromonas salmonicida subsp. salmonicida, a bacterium that causes fish disease, there are two types of small plasmids (<15 kbp): plasmids without known function, called cryptic plasmids, and plasmids that bear beneficial genes for the bacterium. four among them are frequently detected in strains of a. salmonicida subsp. salmonicida: pasa1, pasa2, pasa3, and pasal1. the latter harbors a gene which codes for an effector of the type three secretion system, while the three others are cryptic. it ... | 2017 | 29326751 |
| epidemiology of danish aeromonas salmonicida subsp. salmonicida in fish farms using whole genome sequencing. | furunculosis, a serious infection caused by the bacterium aeromonas salmonicida subsp. salmonicida is common in sea-reared rainbow trout production in denmark. developing an effective control strategy requires knowledge of the epidemiology, as well as the genomic and virulent variability of the danish a. salmonicida subsp. salmonicida isolates. to obtain this, the genomes of 101 a. salmonicida subsp. salmonicida, including 99 danish isolates, one scottish strain and the type strain ncimb 1102, w ... | 2017 | 29259599 |
| interplay between daily rhythmic serum-mediated bacterial killing activity and immune defence factors in rainbow trout (oncorhynchus mykiss). | circadian rhythm is emerging as an important regulator of immune functions. however, there is a paucity of information on the influence of this biological phenomenon in the antimicrobial factors in teleost fish. this study investigated the dynamics and interplay of serum-mediated bacterial killing activity and immune defence factors throughout the light:dark (ld) cycle in rainbow trout (oncorhynchus mykiss). the juvenile fish came from two different emergence time fractions (i.e., late and early ... | 2018 | 29146445 |
| draft genome sequence of the type strain aeromonas salmonicida subsp. salmonicida atcc 33658. | here, we report the draft genome sequence of the type strain aeromonas salmonicida subsp. salmonicida atcc 33658 isolated from salmo salar the size of the genome is 4,728,143 bp with a g+c content of 58.5%. the a. salmonicida subsp. salmonicida atcc 33658 genome lacks essential virulence genes that were likely lost during genomic rearrangements. | 2017 | 28982999 |
| characterization and diversity of phages infecting aeromonas salmonicida subsp. salmonicida. | phages infecting aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 a. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. this bacterial set als ... | 2017 | 28765570 |
| in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens. | antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ... | 2017 | 28732514 |
| alternatives to mineral oil adjuvants in vaccines against aeromonas salmonicida subsp. salmonicida in rainbow trout offer reductions in adverse effects. | in an effort to reduce the frequency and severity of adverse reactions seen from the use of mineral oil adjuvants in salmonid fish, the effects of two alternative adjuvants were assessed, focusing on the induction of adverse effects as well as protection. using rainbow trout (oncorhynchus mykiss) as recipients, injection vaccines based on formalin-inactivated aeromonas salmonicida subspecies salmonicida were formulated with cpg oligodeoxynucleotides, the liposomal cationic adjuvant formulation 0 ... | 2017 | 28724973 |
| plasmid composition in aeromonas salmonicida subsp. salmonicida 01-b526 unravels unsuspected type three secretion system loss patterns. | aeromonas salmonicida subsp. salmonicida is a ubiquitous psychrophilic waterborne bacterium and a fish pathogen. the numerous mobile elements, especially insertion sequences (is), in its genome promote rearrangements that impact its phenotype. one of the main virulence factors of this bacterium, its type three secretion system (ttss), is affected by these rearrangements. in aeromonas salmonicida subsp. salmonicida most of the ttss genes are encoded in a single locus on a large plasmid called pas ... | 2017 | 28701230 |
| analysis of the genome and mobilome of a dissimilatory arsenate reducing aeromonas sp. o23a reveals multiple mechanisms for heavy metal resistance and metabolism. | aeromonas spp. are among the most ubiquitous microorganisms, as they have been isolated from different environmental niches including waters, soil, as well as wounds and digestive tracts of poikilothermic animals and humans. although much attention has been paid to the pathogenicity of aeromonads, the role of these bacteria in environmentally important processes, such as transformation of heavy metals, remains to be discovered. therefore, the aim of this study was a detailed genomic characteriza ... | 2017 | 28611742 |
| differential effects of exposure to parasites and bacteria on stress response in turbot scophthalmus maximus simultaneously stressed by low water depth. | the stress response of turbot scophthalmus maximus was evaluated in fish maintained 8 days under different water depths, normal (nwd, 30 cm depth, total water volume 40 l) or low (lwd, 5 cm depth, total water volume 10 l), in the additional presence of infection-infestation of two pathogens of this species. this was caused by intraperitoneal injection of sublethal doses of the bacterium aeromonas salmonicida subsp. salmonicida or the parasite philasterides dicentrarchi (ciliophora:scuticociliati ... | 2017 | 28516502 |
| the protective effect of humic-rich substances on atypical aeromonas salmonicida subsp. salmonicida infection in common carp (cyprinus carpio l.). | when challenged with atypical aeromonas salmonicida subsp. salmonicida, exposure of the common carp (cyprinus carpio l.) to different humic-rich compounds resulted in a significant reduction in infection rates. specifically, in fish exposed to (i) humic-rich water and sludge from a recirculating system, (ii) a synthetic humic acid, and (iii) a leonardite-derived humic-rich extract, infection rates were reduced to 14.9%, 17.0% and 18.8%, respectively, as compared to a 46.8% infection rate in the ... | 2017 | 28493490 |
| antimicrobial effect of the biotronic® top3 supplement and efficacy in protecting rainbow trout (oncorhynchus mykiss) from infection by aeromonas salmonicida subsp. salmonicida. | demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. in the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and biomin® permeabilizing complex. this mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including aeromonas salmonicida. bacteria ... | 2017 | 28343146 |
| draft genome sequence of pseudomonas fluorescens ml11a, an endogenous strain from brook charr with antagonistic properties against aeromonas salmonicida subsp. salmonicida. | pseudomonas fluorescens ml11a, isolated from brook charr, showed a strong in vitro inhibitory effect against aeromonas salmonicida subsp. salmonicida, a bacterial fish pathogen. its genome harbors gene clusters for siderophore and bacteriocin biosynthesis and shares 99% whole-genome identity with p. fluorescens a506, a biological control strain used in agriculture. | 2017 | 28254983 |
| subunit vaccine candidates against aeromonas salmonicida in rainbow trout oncorhynchus mykiss. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis and a major fish health problem in salmonid aquaculture worldwide. injection vaccination with commercial mineral oil-adjuvanted bacterin vaccines has been partly successful in preventing the disease but in danish rainbow trout (oncorhynchus mykiss, walbaum) aquaculture furunculosis outbreaks still occur. in this study we tested the efficacy of experimental subunit vaccines against a. salmonicida infection in rainbo ... | 2017 | 28182704 |
| identification of the ferric-acinetobactin outer membrane receptor in aeromonas salmonicida subsp. salmonicida and structure-activity relationships of synthetic acinetobactin analogues. | aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in several fish species, produces acinetobactin and amonabactin as siderophores. in a previous study, we chemically characterized these siderophores and proposed a biosynthetic pathway based on genetic analysis. however, the internalization mechanisms of ferric-acinetobactin and ferric-amonabactin remain largely unknown. in the present study, we demonstrate that the outer membrane protein fstb is the ferric-acinetobact ... | 2017 | 27936588 |
| aeromonas salmonicida type iii secretion system-effectors-mediated immune suppression in rainbow trout (oncorhynchus mykiss). | aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. together with other pathogens, it is characterized by the presence of a type 3 secretion system (t3ss). the t3ss is the main virulence mechanism of a. salmonicida. it is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. some of these factors have a detrimental impact on the integrity of the cell cytoskeleton, likely contributin ... | 2017 | 27923746 |
| development of a sybr green i real-time pcr assay for specific identification of the fish pathogen aeromonas salmonicida subspecies salmonicida. | a sybr green i real-time polymerase chain reaction protocol for specific detection of the fish pathogen aeromonas salmonicida subsp. salmonicida was developed and validated for rapid diagnosis of typical furunculosis. the sequence of the aopo gene of a. salmonicida subsp. salmonicida, which encodes for a serine/threonine protein kinase linked to virulence, was chosen for primer design. the selected primers amplified a 119-bp internal fragment of the aopo gene. the specificity test proved that 10 ... | 2016 | 27838837 |
| preliminary study on the in vitro and in vivo effects of asparagopsis taxiformis bioactive phycoderivates on teleosts. | several compounds from marine organisms have been studied for their potential use in aquaculture. among the red algae, asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. depending on the seasonal period of sampling, ethanol extracts of a. taxiformis exhibited signi ... | 2016 | 27826246 |
| the mosaic architecture of aeromonas salmonicida subsp. salmonicida pasa4 plasmid and its consequences on antibiotic resistance. | aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in salmonids, is an issue especially because many isolates of this bacterium display antibiotic resistances, which limit treatments against the disease. recent results suggested the possible existence of alternative forms of pasa4, a large plasmid found in a. salmonicida subsp. salmonicida and bearing multiple antibiotic resistance genes. the present study reveals the existence of two newly detected pasa4 variants, pas ... | 2016 | 27812409 |
| diversity of antibiotic-resistance genes in canadian isolates of aeromonas salmonicida subsp. salmonicida: dominance of psn254b and discovery of pasa8. | the bacterium aeromonas salmonicida subsp. salmonicida is a common pathogen in fish farms worldwide. since the antibiotic resistance of this bacterial species is on the increase, it is important to have a broader view on this issue. in the present study, we tested the presence of known plasmids conferring multi-drug resistance as well as antibiotic resistance genes by a pcr approach in 100 canadian a. salmonicida subsp. salmonicida isolates. our study highlighted the dominance of the conjugative ... | 2016 | 27752114 |
| positive correlation between aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout oncorhynchus mykiss evaluated by a tail fin infection model. | rainbow trout, oncorhynchus mykiss (walbaum), are able to raise a protective immune response against aeromonas salmonicida subsp. salmonicida (as) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under danish mariculture conditions. we elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. rainbow trout juveniles were vaccinated by intra ... | 2017 | 27593619 |
| aeromonas salmonicida subsp. salmonicida strains isolated from chinese freshwater fish contain a novel genomic island and possible regional-specific mobile genetic elements profiles. | two strains of aeromonas salmonicida, yk and bg, were isolated from largemouth bronze gudgeon and northern whitefish in china, and identified as a. salmonicida subsp. salmonicida based on phylogenetic analysis of vapa and 16s rrna gene sequences. yk and bg originated from freshwater fish, one of which belonged to the cyprinid family, and the strains showed a difference in virulence. subsequently, we performed whole genome sequencing of the strains, and comparison of their genomic sequences to th ... | 2016 | 27493011 |
| immunohistochemical study of inducible nitric oxide synthase and tumour necrosis factor alpha response in turbot (scophthalmus maximus) experimentally infected with aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida represents one of the major threats in aquaculture, especially in salmonid fish and turbot farming. in order to fight bacterial infections, fish have an immune system composed by innate and specific cellular and humoral elements analogous to those present in mammals. however, innate immunity plays a primordial role against bacterial infections in teleost fish. among these non-specific mechanisms, the production of nitric oxide (no) by inducible nitric oxi ... | 2016 | 27431586 |
| detection and quantification of aeromonas salmonicida in fish tissue by real-time pcr. | furunculosis, a septicaemic infection caused by the bacterium aeromonas salmonicida subsp. salmonicida, currently causes problems in danish seawater rainbow trout production. detection has mainly been achieved by bacterial culture, but more rapid and sensitive methods are needed. a previously developed real-time pcr assay targeting the plasmid encoded aopp gene of a. salmonicida was, in parallel with culturing, used for the examination of five organs of 40 fish from danish freshwater and seawate ... | 2017 | 27193829 |
| antibiotic resistance due to an unusual cole1-type replicon plasmid in aeromonas salmonicida. | aeromonas salmonicida subsp. salmonicida is a fish pathogen known to have a rich plasmidome. in the present study, we discovered an isolate of this bacterium bearing an additional unidentified small plasmid. after having sequenced the dna of that isolate by next-generation sequencing, it appeared that the new small plasmid is a cole1-type replicon plasmid, named here pasa7. this plasmid bears a functional chloramphenicol-acetyltransferase-encoding gene (cat-pasa7) previously unknown in a. salmon ... | 2016 | 27028891 |
| development of similar broth microdilution methods to determine the antimicrobial susceptibility of flavobacterium columnare and f. psychrophilum. | flavobacterium columnare and f. psychrophilum are major fish pathogens that cause diseases that may require antimicrobial therapy. choice of appropriate treatment is dependent upon determining the antimicrobial susceptibility of isolates. therefore we optimized methods for broth microdilution testing of f. columnare and f. psychrophilum to facilitate standardizing an antimicrobial susceptibility test. we developed adaptations to make reproducible broth inoculums and confirmed the proper incubati ... | 2016 | 26949840 |
| investigations on the role of the salmon louse, lepeophtheirus salmonis (caligidae), as a vector in the transmission of aeromonas salmonicida subsp. salmonicida. | a bacteria-parasite challenge model was used to study the role of sea lice, lepeophtheirus salmonis (copepoda), as a vector of aeromonas salmonicida subsp. salmonicida. three hypotheses were tested: (i) l. salmonis can acquire a. salmonicida subsp. salmonicida via water bath exposure; (ii) l. salmonis can acquire the bacteria via parasitizing infected atlantic salmon, salmo salar; and (iii) l. salmonis can transmit the bacteria to naïve atlantic salmon via parasitism. adult l. salmonis exposed t ... | 2016 | 26851068 |
| characterization of gp21 and gp12: two potential probiotic bacteria isolated from the gastrointestinal tract of atlantic cod. | this study evaluated the probiotic potential of gp21 (pseudomonas sp.) and gp12 (psychrobacter sp.), two bacteria isolated from the intestinal tract of a cold-water fish, atlantic cod. the antagonistic activity of the two intestinal bacteria against two fish pathogens (vibrio anguillarum and aeromonas salmonicida subsp. salmonicida) was studied under different physical conditions. further, their resistance to physiological barriers and their ability to form biofilms were examined. in addition, a ... | 2010 | 26781122 |
| antigens of aeromonas salmonicida subsp. salmonicida specifically induced in vivo in oncorhynchus mykiss. | 2016 | 26661507 | |
| diversity and homogeneity among small plasmids of aeromonas salmonicida subsp. salmonicida linked with geographical origin. | furunculosis, which is caused by aeromonas salmonicida subsp. salmonicida, is a major salmonid disease in fish farms worldwide. several plasmids found in this bacterium confer phenotypes such drug resistance and virulence. small plasmids (pasa1, pasa2, pasa3, and pasal1) related to cole1- and cole2-type replicons are usually present in its normal plasmidome. in the present study, with the objective to investigate if these plasmids display particularities related to the origin of the isolates bea ... | 2015 | 26635745 |
| two catechol siderophores, acinetobactin and amonabactin, are simultaneously produced by aeromonas salmonicida subsp. salmonicida sharing part of the biosynthetic pathway. | the iron uptake mechanisms based on siderophore synthesis used by the fish pathogen aeromonas salmonicida subsp. salmonicida are still not completely understood, and the precise structure of the siderophore(s) is unknown. the analysis of genome sequences revealed that this bacterium possesses two gene clusters putatively involved in the synthesis of siderophores. one cluster is a candidate to encode the synthesis of acinetobactin, the siderophore of the human pathogen acinetobacter baumannii, wh ... | 2015 | 26463084 |
| aeromonas salmonicida infection only moderately regulates expression of factors contributing to toll-like receptor signaling but massively activates the cellular and humoral branches of innate immunity in rainbow trout (oncorhynchus mykiss). | toll-like receptors (tlrs) are known to detect a defined spectrum of microbial structures. however, the knowledge about the specificity of teleost tlr factors for distinct pathogens is limited so far. we measured baseline expression profiles of 18 tlr genes and associated signaling factors in four immune-relevant tissues of rainbow trout oncorhynchus mykiss. intraperitoneal injection of a lethal dose of aeromonas salmonicida subsp. salmonicida induced highly increased levels of cytokine mrnas du ... | 2015 | 26266270 |
| asagei2b: a new variant of a genomic island identified in the aeromonas salmonicida subsp. salmonicida jf3224 strain isolated from a wild fish in switzerland. | aeromonas salmonicida subsp. salmonicida is the causal agent of furunculosis in salmonids. we recently identified a group of genomic islands (asagei) in this bacterium. asagei2a, one of these genomic islands, has almost exclusively been identified in isolates from north america. to date, aeromonas salmonicida subsp. salmonicida jf3224, a strain isolated from a wild brown trout (salmo trutta) caught in switzerland, was the only european isolate that appeared to bear asagei2a. we analyzed the geno ... | 2015 | 26048417 |
| transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida subsp. salmonicida. | furunculosis caused by aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids, including rainbow trout (oncorhynchus mykiss). however, the immune mechanisms that are elicited in rainbow trout against the invasion of a. salmonicida are not yet fully understood. in this study, we examined the spleen to investigate the immune response of rainbow trout at 3days post-infection by a. salmonicida at the transcriptome and proteome levels by using illumina-seq and itraq methods, ... | 2015 | 25849251 |
| draft genome sequences of two aeromonas salmonicida subsp. salmonicida isolates harboring plasmids conferring antibiotic resistance. | the bacterium aeromonas salmonicida is the etiological agent of furunculosis, a widespread fish disease causing important economic losses to the fish farming industry. antibiotic treatments in fish farms may be challenging given the existence of multidrug-resistant isolates of this bacterium. here, we report the draft genome sequences of the 2004-05mf26 and 2009-144k3 isolates, which harbor plasmids conferring antibiotic resistance. both isolates also carry the large plasmid pasa5, which is know ... | 2015 | 25724776 |
| bactericidal efficacy of elevated ph on fish pathogenic and environmental bacteria. | ship ballast water is a recognized medium for transfer and introductions of nonindigenous species. there is a need for new ballast water treatment methods that effectively and safely eliminate or greatly minimize movements of these species. the present study employed laboratory methods to evaluate the bactericidal efficacy of increased ph (ph 10.0-12.0) for exposure durations of up to 72 h to kill a variety of gram-negative and gram-positive bacteria including fish pathogens (aeromonas spp., yer ... | 2012 | 25685439 |
| marine lactobacillus pentosus h16 protects artemia franciscana from vibrio alginolyticus pathogenic effects. | vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. the aim of this study was to assess the probiotic properties and the protective mode of action of lactobacillus pentosus h16 against v. alginolyticus 03/8525, through in vitro and in vivo studies using artemia franciscana (hereafter artemia). this strain showed antimicrobial activity against v. alginolyticus 03/8525 and aeromonas salmonicida subsp. salmonicida atcc33658 possibly related to lactobacill ... | 2015 | 25667335 |
| effective qpcr methodology to quantify the expression of virulence genes in aeromonas salmonicida subsp. salmonicida. | this study aimed to select and validate different methodological strategies to quantify the expression of the virulence genes ascc and ascv by qpcr in aeromonas salmonicida subsp. salmonicida (aer. salmonicida). | 2015 | 25619119 |
| use of in vivo induced antigen technology to identify genes from aeromonas salmonicida subsp. salmonicida that are specifically expressed during infection of the rainbow trout oncorhynchus mykiss. | aeromonas salmonicida is a major fish pathogen associated with mass mortalities in salmonid fish. in the present study, we applied in vivo induced antigen technology (iviat), a technique that relies on antibodies adsorbed against in vitro cultures of the pathogen, to a clinical isolate of a. salmonicida subsp. salmonicida. | 2014 | 25495705 |
| variants of a genomic island in aeromonas salmonicida subsp. salmonicida link isolates with their geographical origins. | aeromonas salmonicida subsp. salmonicida is a fish pathogen. analysis of its genomic characteristics is required to determine the worldwide distribution of the various populations of this bacterium. genomic alignments between the 01-b526 pathogenic strain and the a449 reference strain have revealed a 51-kb chromosomal insertion in 01-b526. this insertion (asagei1a) has been identified as a new genomic island (gei) bearing prophage genes. pcr assays were used to detect this gei in a collection of ... | 2015 | 25480167 |
| triclosan resistance in a bacterial fish pathogen, aeromonas salmonicida subsp. salmonicida, is mediated by an enoyl reductase, fabv. | triclosan, the widely used biocide, specifically targets enoyl-acyl carrier protein reductase (enr) in the bacterial fatty acid synthesis system. although the fish pathogen aeromonas salmonicida subsp. salmonicida exhibits triclosan resistance, the nature of this resistance has not been elucidated. here, we aimed to characterize the triclosan resistance of a. salmonicida subsp. salmonicida causing furunculosis. the fosmid library of triclosan-resistant a. salmonicida subsp. salmonicida was const ... | 2015 | 25370725 |
| aeromonas salmonicida binds differentially to mucins isolated from skin and intestinal regions of atlantic salmon in an n-acetylneuraminic acid-dependent manner. | aeromonas salmonicida subsp. salmonicida infection, also known as furunculosis disease, is associated with high morbidity and mortality in salmonid aquaculture. the first line of defense the pathogen encounters is the mucus layer, which is predominantly comprised of secreted mucins. here we isolated and characterized mucins from the skin and intestinal tract of healthy atlantic salmon and studied how a. salmonicida bound to them. the mucins from the skin, pyloric ceca, and proximal and distal in ... | 2014 | 25287918 |
| adverse and long-term protective effects following oil-adjuvanted vaccination against aeromonas salmonicida in rainbow trout. | prophylactic measures against aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, have been an active field of research for decades, with studies mainly focused on atlantic salmon (salmo salar). in the present study we have examined the protective and adverse effects of mineral oil-adjuvanted injection vaccines on rainbow trout (oncorhynchus mykiss). a commercial vaccine and an experimental auto vaccine, as well as their respective adjuvant formulations alone were used ... | 2015 | 25281580 |
| detection of variants of the pras3, pab5s9, and psn254 plasmids in aeromonas salmonicida subsp. salmonicida: multidrug resistance, interspecies exchanges, and plasmid reshaping. | the ubiquitous water-borne gram-negative bacterium aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a worldwide disease in fish farms. plasmids carrying antibiotic resistance genes have already been described for this bacterium. the aim of the present study was to identify and characterize additional multidrug resistance plasmids in a. salmonicida subsp. salmonicida. we sequenced the plasmids present in two multiple antibiotic-resistant isolates using high-through ... | 2014 | 25267667 |
| the warm temperature acclimation protein (wap65) has an important role in the inflammatory response of turbot (scophthalmus maximus). | wap65 is a molecule similar to the mammalian hemopexin that is a serum glycoprotein produced mainly by the liver with high affinity to heme. its primary role is participating in iron metabolism scavenging heme that is released into the plasma and transporting it to the liver. it has been reported an important role of hemopexin in the inflammation as an acute-phase protein and its production is up-regulated by pro-inflammatory cytokines. there are also some evidences suggesting this immune-induct ... | 2014 | 24794581 |
| optimization of a plasmid electroporation protocol for aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida is a major fish pathogen. molecular tools are required to study the virulence and genomic stability of this bacterium. an efficient electroporation-mediated transformation protocol for a. salmonicida subsp. salmonicida would make genetic studies faster and easier. in the present study, we designed the 4.1-kb psdd1 plasmid as a tool for optimizing an electroporation protocol for a. salmonicida subsp. salmonicida. we systematically tested the electroporatio ... | 2014 | 24389038 |
| comparision of a serological potency assay for furunculosis vaccines (aeromonas salmonicida subsp. salmonicida) to intraperitoneal challenge in atlantic salmon (salmo salar l.). | batch potency testing of salmonid vaccines is mainly performed by in vivo challenge, which requires a lot of animals and causes severe pain. due to the animal welfare concerns associated with in vivo immunization challenge tests, methods which could refine, reduce or replace (3rs) these tests are needed. the aim of this study was to assess the use of serological assay (immunization & antibody estimation with an enzyme-linked immunosorbent assay (elisa) for batch potency testing of oil adjuvanted ... | 2014 | 24359994 |
| the aeromonas salmonicida subsp. salmonicida exoproteome: global analysis, moonlighting proteins and putative antigens for vaccination against furunculosis. | aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen of fisheries worldwide. despite the identification of several virulence factors the pathogenesis is still poorly understood. we have used high-throughput proteomics to display the differences between in vitro secretome of a. salmonicida wild-type (wt, hypervirulent, jf5054) and t3ss-deficient (isogenic δascv, extremely low-virulent, jf2747) strains in exponential (gp) and stationary (sp) phases of ... | 2013 | 24127837 |
| aeromonas salmonicida subsp. salmonicida in the light of its type-three secretion system. | aeromonas salmonicida subsp. salmonicida is an important pathogen in salmonid aquaculture and is responsible for the typical furunculosis. the type-three secretion system (t3ss) is a major virulence system. in this work, we review structure and function of this highly sophisticated nanosyringe in a. salmonicida. based on the literature as well as personal experimental observations, we document the genetic (re)organization, expression regulation, anatomy, putative functional origin and roles in t ... | 2014 | 24119189 |
| the aeromonas salmonicida subsp. salmonicida exoproteome: determination of the complete repertoire of type-three secretion system effectors and identification of other virulence factors. | aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen of fisheries worldwide. several virulence factors have been described, but the type-three secretion system (t3ss) is recognized as having a major effect on virulence by injecting effectors directly into fish cells. in this study we used high-throughput proteomics to display the differences between in vitro secretome of a. salmonicida wild-type (wt, hypervirulent, jf2267) and t3ss-deficient (isogeni ... | 2013 | 24073886 |
| long-lasting protection induced by bath vaccination against aeromonas salmonicida subsp. salmonicida in rainbow trout. | for decades aeromonas salmonicida subsp. salmonicida (from here referred to as a. salmonicida) has been recognized as the causative agent of typical furunculosis. this disease has had a major impact on aquaculture worldwide, making it a target for international research, particularly within the field of immunoprohylaxis. initial studies attempted vaccination via oral route and immersion. however, these vaccination methods proved insufficient when compared to intraperitoneally (i.p.) injected vac ... | 2013 | 24056280 |
| antigens of the type-three secretion system of aeromonas salmonicida subsp. salmonicida prevent protective immunity in rainbow trout. | aeromonas salmonicida subsp. salmonicida is the etiologic agent of furunculosis, a frequent and significant disease of fisheries worldwide. the disease is largely controlled by commercial oil adjuvanted vaccines containing bacterins. however, the mechanisms leading to a protective immune response remain poorly understood. the type-three secretion system (t3ss) plays a central role in virulence of a. salmonicida subsp. salmonicida and thus may have an influence on the immune response of the host. ... | 2013 | 24012573 |
| insertion sequence as5 (is as5 ) is involved in the genomic plasticity of aeromonas salmonicida. | the genome of the fish pathogen aeromonas salmonicida subsp salmonicida harbors a large number of insertion sequences (iss), many of which are located on plasmids. in the present study, we analyzed the small plasmid profile of a. salmonicida strains to identify evidences of plasmid alterations. ten out of 78 strains analyzed displayed an unconventional plasmid profile. however the her1104 strain was unique, having a positive pcr signal for pasal1 plasmid despite not carrying this plasmid. instea ... | 2013 | 23956951 |
| virulence, genomic features, and plasticity of aeromonas salmonicida subsp. salmonicida, the causative agent of fish furunculosis. | the bacterium aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a systemic disease of fish in the salmonid family. furunculosis is a ubiquitous disease that affects aquaculture operations worldwide and is characterized by high mortality and morbidity. a better understanding of the bacterium is required to find a cure. thereby, this review centers on a. salmonicida subsp. salmonicida, its major virulence factors, and its genome. the classification and characteristic ... | 2014 | 23890675 |
| biological control of aeromonas salmonicida subsp. salmonicida infection in rainbow trout (oncorhynchus mykiss) using aeromonas phage pas-1. | the potential control efficacy of aeromonas phage pas-1 was evaluated against aeromonas salmonicida subsp. salmonicida infection in rainbow trout (oncorhynchus mykiss) model in this study. the phage was co-cultured with the virulent a. salmonicida subsp. salmonicida strain as05 that possesses the type iii secretion system (ttss) ascv gene, and efficient bacteriolytic activity was observed against the bacteria. the administration of pas-1 in rainbow trout demonstrated that the phage was cleared f ... | 2015 | 23594036 |
| polyphasic characterization of aeromonas salmonicida isolates recovered from salmonid and non-salmonid fish. | michigan's fisheries rely primarily upon the hatchery propagation of salmonid fish for release in public waters. one limitation on the success of these efforts is the presence of bacterial pathogens, including aeromonas salmonicida, the causative agent of furunculosis. this study was undertaken to determine the prevalence of a. salmonicida in michigan fish, as well as to determine whether biochemical or gene sequence variability exists among michigan isolates. a total of 2202 wild, feral and hat ... | 2013 | 23444966 |
| quality control ranges for testing broth microdilution susceptibility of flavobacterium columnare and f. psychrophilum to nine antimicrobials. | a multi-laboratory broth microdilution method trial was performed to standardize the specialized test conditions required for the fish pathogens flavobacterium columnare and f. psychrophilum. nine laboratories tested the quality control (qc) strains escherichia coli atcc 25922 and aeromonas salmonicida subsp. salmonicida atcc 33658 against 10 antimicrobials (ampicillin, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, ormetoprim/sulfadimethoxine, oxolinic acid, oxytetracycline, a ... | 2012 | 23324417 |
| the hydrocarbon-degrading marine bacterium cobetia sp. strain mm1ida2h-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking. | biosurfactants are produced by hydrocarbon-degrading marine bacteria in response to the presence of water-insoluble hydrocarbons. this is believed to facilitate the uptake of hydrocarbons by bacteria. however, these diffusible amphiphilic surface-active molecules are involved in several other biological functions such as microbial competition and intra- or inter-species communication. we report the isolation and characterization of a marine bacterial strain identified as cobetia sp. mm1ida2h-1, ... | 2013 | 23279885 |
| analysis of a ferric uptake regulator (fur) knockout mutant in aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis; a serious infectious disease in aquaculture raised salmonids. iron acquisition has been shown to be critical for the survival of pathogenic bacteria during the course of infection. previous work has demonstrated that a. salmonicida expresses iron-repressible iromp proteins, suggesting the presence of iron acquisition systems that are under the control of a ferric uptake regulator (fur). in this study, the a. salmo ... | 2013 | 23228622 |
| antibody responses correlate with antigen dose and in vivo protection for oil-adjuvanted, experimental furunculosis (aeromonas salmonicida subsp. salmonicida) vaccines in atlantic salmon (salmo salar l.) and can be used for batch potency testing of vaccines. | salmon farming has increased dramatically over last thirty years and a key to the success is the introduction of protective vaccines. in norway, almost 100% of all atlantic salmon are vaccinated prior to sea transfer. this extensive use of vaccines demands use of a lot of resources in production and quality control of vaccines, and fish are now one of the most widely used laboratory animal species in norway, since all batch testing today is performed by challenge experiments. with an increasing ... | 2013 | 23219439 |
| potential role of specific antibodies as important vaccine induced protective mechanism against aeromonas salmonicida in rainbow trout. | furunculosis caused by infection with aeromonas salmonicida subsp. salmonicida has been a known threat to aquaculture for more than a century. efficient prophylactic approaches against this disease are essential for continued growth of salmonid aquaculture. since the introduction of successful oil-adjuvanted vaccines in the early 1990's, a number of studies have been published on the protective as well as adverse effects of these vaccines. most studies focus on vaccination of salmon (salmo salar ... | 2012 | 23056427 |
| first description of cole-type plasmid in aeromonas spp. carrying quinolone resistance (qnrs2) gene. | isolation and full sequence analysis of cole-type plasmid, which carries the qnrs2 gene. | 2012 | 22862417 |
| virulence factors of aeromonas salmonicida subsp. salmonicida strains associated with infections in turbot psetta maxima. | virulence factors for aeromonas salmonicida subsp. salmonicida (ass) strains isolated from cultured turbot psetta maxima l. are unknown with regard to this host. the presence of virulence genes associated with different stages of ass infection in salmonids (vapa, tapa, fla, ascv, ascc, aext, sata and aspa) was analysed using a polymerase chain reaction (pcr) technique in ass strains isolated from turbot. other ass strains isolated from salmonids and environmental a. salmonicida (as) strains were ... | 2012 | 22691983 |
| complete genome sequence of bacteriophage phias7, a t7-like virus that infects aeromonas salmonicida subsp. salmonicida. | to date, a number of myoviridae bacteriophages that infect aeromonadaceae have been identified and characterized. however, the genome sequences of aeromonas phages that not belong to the myoviridae have not been investigated yet. herein, we report the complete genome sequence of aeromonas phage phias7, which belongs to the podoviridae and infects aeromonas salmonicida subsp. salmonicida. | 2012 | 22328699 |
| isolation and characterization of a lytic myoviridae bacteriophage pas-1 with broad infectivity in aeromonas salmonicida. | to search for candidate control agents against aeromonas salmonicida subsp. salmonicida infections in aquaculture, one bacteriophage (phage), designated as pas-1, was isolated from the sediment samples of the rainbow trout (oncorhynchus mykiss) culture farm in korea. the pas-1 was morphologically classified as myoviridae and possessed approximately 48 kb of double-strand genomic dna. the phage showed broad host ranges to other subspecies of a. salmonicida as well as a. salmonicida subsp. salmoni ... | 2012 | 22314935 |
| complete genome sequence and characterization of a broad-host range t4-like bacteriophage phias5 infecting aeromonas salmonicida subsp. salmonicida. | in this study, we report one lytic myoviridae bacteriophage (phage) infecting aeromonas salmonicida subsp. salmonicida. the phage (named as phias5) was isolated from environmental river waters in korea, and showed broad infectivity to other bacterial species in the family aeromonadaceae as well as antibiotic-resistant a. salmonicida subsp. salmonicida strains. the biological properties and complete genome of phias5 were simultaneously investigated. the complete genome of phias5 composed of linea ... | 2011 | 22226819 |
| complete genomic sequence of a t4-like bacteriophage, phias4, infecting aeromonas salmonicida subsp. salmonicida. | a newly identified virulent phage (named phias4) infecting aeromonas salmonicida subsp. salmonicida was isolated from river water in korea. morphological analysis of phias4 by transmission electron microscopy revealed that it belonged to the family myoviridae. the genome of phias4 comprised a linear double-stranded dna of 163,875 bp with a g + c content of 41.3%, and genomic analysis revealed 271 putative orfs, 67 putative promoters, 25 putative terminator regions, and 16 trna-encoding genes. mo ... | 2011 | 22116495 |
| In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture. | Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ... | 2012 | 22078751 |
| development of an antibody elisa for potency testing of furunculosis (aeromonas salmonicida subsp salmonicida) vaccines in atlantic salmon (salmo salar l). | the study was conducted in atlantic salmon to establish the initial and basic scientific documentation for an alternative batch potency test for salmon furuculosis vaccines. we assessed the antibody response development for aeromonas salmonicida vaccines at different immunisation temperatures (3, 12 and 18 °c), by an enzyme-linked-immunosorbent assay (elisa) 3, 6, 9 and 12 weeks post vaccination, and the correlation between antibody response and protection in cohabitation challenge experiments p ... | 2011 | 22000732 |
| Colonization of turbot tissues by virulent and avirulent Aeromonas salmonicida subsp. salmonicida strains during infection. | Preventing disease outbreaks in cultured turbot Psetta maxima L. caused by Aeromonas salmonicida subsp. salmonicida (ASS) requires a better understanding of how this pathogen colonizes its host. Distribution of 1 virulent and 2 avirulent ASS strains in turbot tissues was investigated during early and late stages of infection following an immersion challenge. To track bacteria within the turbot, the ASS strains were tagged with green fluorescent protein (GFP). Both virulent and avirulent strains ... | 2011 | 21848125 |
| enterobacteriaceae associated with eggs of podocnemis expansa and podocnemis unifilis (testudines:chelonia) in nonpolluted sites of national park of araguaia plains, brazil. | fertile eggs of podocnemis expansa and podocnemis unifilis were investigated for the presence of enterobacteria, as these two endangered species have the potential for conservation measures that include egg transfer. knowledge of normal microflora associated with turtles and turtle eggs would help effectively manage the transfer of these eggs among institutions. thirty eggs of each species were collected, aseptically transferred, cracked inside plastic bags containing tetrathionate broth, and sp ... | 2010 | 21370647 |
| in vitro adherence of two candidate probiotics from atlantic cod and their interference with the adhesion of two pathogenic bacteria. | the potential of two candidate probiotic bacteria (gp21 and gp12), isolated from the gut of atlantic cod, to adhere to primary cultures of the epithelial cells from the different regions of the intestine and to interfere with the adhesion of two pathogens, vibrio anguillarum and aeromonas salmonicida subsp. salmonicida were investigated. the intestinal isolates showed clear preference in adhering to the cells from the different intestine segments. gp12 adhered strongly to the fore- and mid intes ... | 2010 | 20884135 |
| development and evaluation of a multiplex pcr assay for simultaneous detection of flavobacterium psychrophilum, yersinia ruckeri and aeromonas salmonicida subsp. salmonicida in culture fisheries. | bacterial cold water disease, enteric red mouth disease and frunculosis are the common bacterial diseases of fish worldwide. the etiologic agents of these diseases are flavobacterium (f.) psychrophilum, yersinia (y.) ruckeri and aeromonas (a.) salmonicida subsp. salmonicida, respectively. in this study, a multiplex polymerase chain reaction (m-pcr) method with yer8/10-fer3/4-fp1/3 primer pairs which can identify these fish pathogens simultaneously was developed and optimized. in optimized condit ... | 2010 | 20706031 |
| defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ... | 2010 | 20537393 |
| thiaminase activity of crucian carp carassius carassius injected with a bacterial fish pathogen, aeromonas salmonicida subsp. salmonicida. | dietary thiaminase i is a cause of thiamine deficiency in animals. the physiological significance of thiaminase in the organisms containing this enzyme is not known, nor are the factors causing variation in their thiaminase activity. tests were performed to evaluate the effect a pathogen might have on thiaminase activity in fish, when analyzed both with a cosubstrate added (cata tests) and no cosubstrate added (ncata tests). pyridine is known as a cosubstrate specific for thiaminase i activity t ... | 2009 | 20218496 |
| multiplex pcr method for detection of three aeromonas enterotoxin genes. | a novel multiplex pcr method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in aeromonas spp. this assay was used to characterize 35 reference strains as well as 537 food-borne isolates. a total of seven gene pattern combinations were encountered, including act, alt, act/alt, act/alt/ast, act/alt/148-bp amplicon, alt/ast, and alt/148-bp amplicon. the alt gene was detected with 34 reference st ... | 2010 | 19933350 |
| mutations in the aeromonas salmonicida subsp. salmonicida type iii secretion system affect atlantic salmon leucocyte activation and downstream immune responses. | deletion mutants of aeromonas salmonicida subsp. salmonicida were used to determine the effect of the type three secretion system (ttss) on atlantic salmon anterior head kidney leucocytes (ahkl). one strain had a deletion in the outer membrane pore gene, ascc; and the other in three effector genes: aopo, aoph and aext (we call this strain deltaaop3). host cell invasion success and 24h survival were depressed in deltaascc, as was 24h survival of deltaaop3, when compared to the wild type strain. c ... | 2009 | 19751835 |
| aeromonas salmonicida subsp. salmonicida type strain does not possess a type iii secretion system. | 2009 | 19553573 | |
| plasma ladderlectin concentration following sterile inflammation and aeromonas salmonicida subsp. salmonicida infection. | plasma samples obtained from rainbow trout either experimentally infected with aeromonas salmonicida or injected with either a. salmonicida lipopolysaccharide (lps) or a commercial a. salmonicida vaccine (lipogen) were analysed by enzyme immunoassay to evaluate changes in rainbow trout ladderlectin (rtll) concentrations during the acute phase response (apr). plasma rtll concentrations in fish injected with a. salmonicida lps, vaccine or live a. salmonicida varied over a 10 day period, but did no ... | 2009 | 19538251 |
| quantitative analysis of bacterial adhesion to fish tissue. | adhesion to host tissue represents a first crucial step in most bacterial infections. both specific adhesion-ligand as well as hydrophobic interactions may be involved. the adhesion of aeromonas salmonicida subsp. salmonicida, lactococcus garvieae, and yersinia ruckeri strains to fish tissue cells was assessed. to determine whether the observed bacterial adhesion to fish tissue cells was caused by non-specific interactions, adhesion to bovine serum albumin (bsa) and polystyrene was also tested. ... | 2009 | 19349152 |
| genetic characterization of pasa6, a new plasmid from aeromonas salmonicida subsp. salmonicida that encodes a type iii effector protein aoph homolog. | a new plasmid designated pasa6 from an aeromonas salmonicida subsp. salmonicida strain isolated from diseased turbot has been characterized. pasa6 consists of 18536bp, has a g+c content of 53.8% and encodes 20 predicted open-reading frames (orfs). eight orfs showed homology to transposases, of which six are complete and two are partial is sequences. two orfs showed homology to replication proteins, and six orfs showed homology to hypothetical proteins. two orfs are truncated homologs of putative ... | 2009 | 19195484 |
| genetics and proteomics of aeromonas salmonicida lipopolysaccharide core biosynthesis. | comparison between the lipopolysaccharide (lps) core structures of aeromonas salmonicida subsp. salmonicida a450 and aeromonas hydrophila ah-3 shows great similarity in the inner lps core and part of the outer lps core but some differences in the distal part of the outer lps core (residues ld-hep, d-gal, and d-galnac). the three genomic regions encoding lps core biosynthetic genes in a. salmonicida a450, of which regions 2 and 3 have genes identical to those of a. hydrophila ah-3, were fully seq ... | 2009 | 19151135 |
| the genome of aeromonas salmonicida subsp. salmonicida a449: insights into the evolution of a fish pathogen. | aeromonas salmonicida subsp. salmonicida is a gram-negative bacterium that is the causative agent of furunculosis, a bacterial septicaemia of salmonid fish. while other species of aeromonas are opportunistic pathogens or are found in commensal or symbiotic relationships with animal hosts, a. salmonicida subsp. salmonicida causes disease in healthy fish. the genome sequence of a. salmonicida was determined to provide a better understanding of the virulence factors used by this pathogen to infect ... | 2008 | 18801193 |
| surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006. | the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ... | 2008 | 18786028 |
| identification of iron regulated genes in the fish pathogen aeromonas salmonicida subsp. salmonicida: genetic diversity and evidence of conserved iron uptake systems. | in this study we applied the fur titration assay (furta) to isolate iron regulated genes in aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish. we have identified genes for siderophore biosynthesis and for ferri-siderophore transport, some of which have been previously described in this species. a gene for a hitherto uncharacterized functional hemin receptor hute was identified, but its inactivation did not affect significantly the use of hemin as sole iron sou ... | 2009 | 18774242 |
| insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology. | wastewater treatment plants (wwtps) are a reservoir for bacteria harbouring antibiotic resistance plasmids. to get a comprehensive overview on the plasmid metagenome of wwtp bacteria showing reduced susceptibility to certain antimicrobial drugs an ultrafast sequencing approach applying the 454-technology was carried out. one run on the gs 20 system yielded 346,427 reads with an average read length of 104 bases resulting in a total of 36,071,493 bases sequence data. the obtained plasmid metagenom ... | 2008 | 18586057 |
| identification of heme uptake genes in the fish pathogen aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish, can use heme as the sole iron source. we applied the fur titration assay to isolate a cluster including six genes hutazxbcd that showed similarity to heme uptake genes of other gram-negative bacteria, and three genes orf123 of unknown function. the spatial organization of these nine genes, arranged in five transcriptional units, was similar to that of a homologous cluster in a. hydrophila. when a tonb system w ... | 2008 | 18535817 |
| development of multiplex pcr assay for simultaneous detection of five bacterial fish pathogens. | a multiplex polymerase chain reaction (pcr) method was designed for the simultaneous detection of the five major fish pathogens, aeromonas hydrophila, aeromonas salmonicida subsp. salmonicida, flavobacterium columnare, renibacterium salmoninarum, and yersinia ruckeri. each of the five pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. the detection limits of the multiplex pcr was in the range of 2, 1, 1, 3, and 1cfu for a. hydrophila, a. salmo ... | 2008 | 18499358 |
| distribution of small plasmids in aeromonas salmonicida subsp. salmonicida strains isolated from nw spain and portugal: evidence of clonality in strains isolated from turbot, psetta maxima (l.). | 2008 | 18471103 | |
| transferable, multiple antibiotic and mercury resistance in atlantic canadian isolates of aeromonas salmonicida subsp. salmonicida is associated with carriage of an inca/c plasmid similar to the salmonella enterica plasmid psn254. | the aim of this study was to examine the molecular basis for multiple antibiotic and mercury resistance in canadian isolates of aeromonas salmonicida subsp. salmonicida. | 2008 | 18375380 |
| identification of siderophore biosynthesis genes essential for growth of aeromonas salmonicida under iron limitation conditions. | aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, produces a catechol-type siderophore under iron-limiting conditions. in this study, the fur titration assay (furta) was used to identify a cluster of six genes, asbg, asbf, asbd, asbc, asbb, and asbi, encoding proteins similar to components of the siderophore biosynthetic machinery in other bacteria. reverse transcriptase pcr analyses showed that this cluster consists of four iron-regulated transcriptional u ... | 2008 | 18296539 |
| contribution of type iv pili to the virulence of aeromonas salmonicida subsp. salmonicida in atlantic salmon (salmo salar l.). | aeromonas salmonicida subsp. salmonicida, a bacterial pathogen of atlantic salmon, has no visible pili, yet its genome contains genes for three type iv pilus systems. one system, tap, is similar to the pseudomonas aeruginosa pil system, and a second, flp, resembles the actinobacillus actinomycetemcomitans flp pilus, while the third has homology to the mannose-sensitive hemagglutinin pilus of vibrio cholerae. the latter system is likely nonfunctional since eight genes, including the gene encoding ... | 2008 | 18212071 |
| in vitro studies of lactobacillus delbrueckii subsp. lactis in atlantic salmon (salmo salar l.) foregut: tissue responses and evidence of protection against aeromonas salmonicida subsp. salmonicida epithelial damage. | probiotic bacteria increase the host health status and protect mucosal tissue against pathogen-caused damage in mammalian models. using an in vitro (intestinal sac) method this study aimed to address (a) the in vitro ability of lactobacillus delbrueckii subsp. lactis to remain in the gastrointestinal tract of atlantic salmon (salmo salar l.) and (b) its ability to prevent cellular damage caused by successive incubation with aeromonas salmonicida subsp. salmonicida the causative agent of furuncul ... | 2008 | 18054448 |
| isolation of aeromonas salmonicida from sea lamprey (petromyzon marinus) with furuncle-like lesions in lake ontario. | for the past six decades, parasitic sea lampreys (petromyzon marinus) have caused devastating losses to salmonid fisheries in the great lakes. to reduce the number of sea lampreys, the great lakes fishery commission began a large-scale program based on trapping male sea lampreys, sterilizing them, and releasing sterile males back into streams to compete with fertile males for spawning females. the transfer of lampreys among lakes can potentially lead to the transfer of various pathogens, and thi ... | 2007 | 17984256 |
| bacterial-binding activity and plasma concentration of ladderlectin in rainbow trout (oncorhynchus mykiss). | soluble, defense lectins bind conserved microbial patterns leading to pathogen opsonization, enhanced phagocytosis and activation of complement. these immune functions, however, vary widely among individuals due to genetic and acquired differences affecting binding capacity or plasma concentration. most evidence for the defensive function of soluble lectins is based on mammals, but several functionally homologous, but less well-characterized, lectins have been identified in fish. in this study, ... | 2007 | 17383895 |
| probiotic properties of lactobacillus and bifidobacterium strains isolated from porcine gastrointestinal tract. | one strain of lactobacillus salivarius, two strains of lactobacillus reuteri and lactobacillus amylovorus, and two strains of bifidobacterium thermacidophilum with antagonistic effect against clostridium perfringens were isolated from porcine gastrointestinal tract. isolates were assayed for their ability to survive in synthetic gastric juice at ph 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. there was a large variation in the survival of the iso ... | 2007 | 17136367 |
| characterization of twin toll-like receptors from rainbow trout (oncorhynchus mykiss): evolutionary relationship and induced expression by aeromonas salmonicida salmonicida. | structure and function of factors contributing to the innate immune system of lower vertebrates, including fish are only sparsely characterized. we retrieved with rt-pcr cdna copies of two closely related toll-like receptors (tlr) from liver rna of the rainbow trout (oncorhynchus mykiss). the cdna sequences are homologous to 95.6%. the phylogenetic analysis of their deduced amino acid sequences places these twin factors closely to other known tlrs from fish. the twin factors are equally expresse ... | 2007 | 17070576 |
| structural characterization of the lipid a region of aeromonas salmonicida subsp. salmonicida lipopolysaccharide. | the lipid a components of aeromonas salmonicida subsp. salmonicida from strains a449, 80204-1 and an in vivo rough isolate were isolated by mild acid hydrolysis of the lipopolysaccharide. structural studies carried out by a combination of fatty acid, electrospray ionization-mass spectrometry and nuclear magnetic resonance analyses confirmed that the structure of lipid a was conserved among different isolates of a. salmonicida subsp. salmonicida. all analyzed strains contained three major lipid a ... | 2006 | 17049500 |
| aopp, a type iii effector protein of aeromonas salmonicida, inhibits the nf-kappab signalling pathway. | aeromonas salmonicida subsp. salmonicida contains a functional type iii secretion system that is responsible for the secretion of the adp-ribosylating toxin aext. in this study, the authors identified aopp as a second effector protein secreted by this system. the aopp gene was detected in both typical and atypical a. salmonicida isolates and was found to be encoded on a small plasmid of approximately 6.4 kb. sequence analysis indicates that aopp is a member of the yopj family of effector protein ... | 2006 | 16946275 |
| contribution of the type iii secretion system (ttss) to virulence of aeromonas salmonicida subsp. salmonicida. | the recently described type iii secretion system (ttss) of aeromonas salmonicida subsp. salmonicida has been linked to virulence in salmonids. in this study, three ttss effector genes, aext, aoph or aopo, were inactivated by deletion, as was ascc, the gene encoding the outer-membrane pore of the secretion apparatus. effects on virulence were assayed by live challenge of atlantic salmon (salmo salar). the deltaascc mutant strain was avirulent by both intraperitoneal (i.p.) injection and immersion ... | 2006 | 16735747 |