Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| whole-genome sequences of brucella melitensis strain qy1, isolated from sheep in gansu, china. | the facultative intracellular gram-negative bacterium brucella melitensis causes brucellosis in domestic and wild mammals, and it is a dominant pathogen responsible for human disease. this study reports the whole-genome sequencing of b. melitensis strain qy1, isolated from sheep suffering from abortion and arthritis in 2015 in gansu, china. | 2017 | 28860252 |
| trypanosoma infection favors brucella elimination via il-12/ifnγ-dependent pathways. | this study develops an original co-infection model in mice using brucella melitensis, the most frequent cause of human brucellosis, and trypanosoma brucei, the agent of african trypanosomiasis. although the immunosuppressive effects of t. brucei in natural hosts and mice models are well established, we observed that the injection of t. brucei in mice chronically infected with b. melitensis induces a drastic reduction in the number of b. melitensis in the spleen, the main reservoir of the infecti ... | 2017 | 28824630 |
| caprine brucellosis: a historically neglected disease with significant impact on public health. | caprine brucellosis is a chronic infectious disease caused by the gram-negative cocci-bacillus brucella melitensis. middle- to late-term abortion, stillbirths, and the delivery of weak offspring are the characteristic clinical signs of the disease that is associated with an extensive negative impact in a flock's productivity. b. melitensis is also the most virulent brucella species for humans, responsible for a severely debilitating and disabling illness that results in high morbidity with inter ... | 2017 | 28817647 |
| systems biology analysis of temporal in vivo brucella melitensis and bovine transcriptomes predicts host:pathogen protein-protein interactions. | to date, fewer than 200 gene-products have been identified as brucella virulence factors, and most were characterized individually without considering how they are temporally and coordinately expressed or secreted during the infection process. here, we describe and analyze the in vivo temporal transcriptional profile of brucella melitensis during the initial 4 h interaction with cattle. pathway analysis revealed an activation of the "two component system" providing evidence that the in vivo bruc ... | 2017 | 28798726 |
| genome sequences of brucella melitensis, isolated from blood samples of brucellosis patients in malaysia. | human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. brucella melitensis has caused outbreaks and sporadic cases in malaysia. here, we present the whole-genome sequences of four b. melitensis strains isolated from brucellosis patients in malaysia. | 2017 | 28774972 |
| [hoof genotyping characteristics of brucella melitensis strains isolated in ulanqab of inner mongolia autonomous region]. | objective: to investigate the hoof genotyping characteristics of 83 brucella (b.) melitensis strains isolated in ulanqab of inner mongolia autonomous region from 2012 to 2015. methods: a total of 83 b. melitensis strains were detected by convention identification and amos-pcr, then hoof protocol with eight vntr locus were used for the genotyping of the strains, and the allelic diversity of each vntr locus and the discriminatory power of vntr typing of hoof were assessed by hunter-gaston discrimi ... | 2017 | 28738473 |
| using real-time polymerase chain reaction as an alternative rapid method for enumeration of colony count in live brucella vaccines. | brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. it has economic, public health, and bio-risk importance. control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. there are three types of animal brucella live vaccines - brucella melitensis rev-1 vaccine, brucella abortus s19, and b. abortus rb51. evaluation of these vaccines depe ... | 2017 | 28717311 |
| brucella infection in asian sea otters (enhydra lutris lutris) on bering island, russia. | infection with brucella spp., long known as a cause of abortion, infertility, and reproductive loss in domestic livestock, has increasingly been documented in marine mammals over the past two decades. we report molecular evidence of brucella infection in asian sea otters (enhydra lutris lutris). brucella dna was detected in 3 of 78 (4%) rectal swab samples collected between 2004 and 2006 on bering island, russia. these 78 animals had previously been documented to have a brucella seroprevalence o ... | 2017 | 28715292 |
| comparative study of immunopathophysiological responses induced by b. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure. | in this study, we developed a mouse model and characterized the effects of intranasal inoculation of virulent brucella melitensis strain 16m and its lipopolysaccharide (lps). the effects of the exposure were compared with respective control groups. both brucella melitensis-infected and lps-infected groups showed no significant clinical presentation with minor relevance in the mortality associated with the infection. in brucella melitensis-infected group, significant histopathological changes in ... | 2017 | 28710016 |
| brucella pericarditis: a forgotten cause of chest pain. | brucellosis pericarditis is an extremely rare involvement of the cardiovascular system with brucella species that has worldwide distribution and is endemic in many provinces of iran. | 2017 | 28702152 |
| in vitro synergistic effects of a short cationic peptide and clinically used antibiotics against drug-resistant isolates of brucella melitensis. | in the last few decades, increasing microbial resistance to common antibiotics has attracted researchers' attention to the development of new classes of antibiotics such as antimicrobial peptides. accordingly, the aim of the current study was to evaluate antimicrobial effects of the cm11 peptide alone and combined with common antibiotics against drug-resistant isolates of brucella melitensis. | 2017 | 28699872 |
| human brucella melitensis infections in southern vietnam. | 2017 | 28669842 | |
| comparative diagnostic evaluation of omp31 gene based taqman® real-time pcr assay with visual lamp assay and indirect elisa for caprine brucellosis. | brucellosis is one of the leading causes of abortion in domestic animals that imposes costs on both economy and society. the disease is highly zoonotic and poses risk to animal handlers due to its zoonotic nature. it causes stillbirth, loss of kids and abortion in last term of pregnancy. reproductive damage includes infertility in does and orchitis and epididymitis in breeding bucks, which result in high financial losses to farmers and the agriculture industry as a whole. it requires highly sens ... | 2017 | 28638960 |
| biochemical characterization of wbkc, an n-formyltransferase from brucella melitensis. | it has become increasingly apparent within the last several years that unusual n-formylated sugars are often found on the o-antigens of such gram negative pathogenic organisms as francisella tularensis, campylobacter jejuni, and providencia alcalifaciens, among others. indeed, in some species of brucella, for example, the o-antigen contains 1,2-linked 4-formamido-4,6-dideoxy-α-d-mannosyl groups. these sugars, often referred to as n-formylperosamine, are synthesized in pathways initiating with gd ... | 2017 | 28636341 |
| mucosal bacterial dissemination in a rhesus macaque model of experimental brucellosis. | animals were experimentally infected with brucella melitensis via aerosol. b. melitensis was cultured from the saliva and vaginal vault of infected animals, corresponding to bacterial dissemination in other target tissues. this is the first report of bacterial dissemination to these mucosal surfaces in a non-human primate model of brucellosis. | 2017 | 28573738 |
| endogenous brucella endophthalmitis: a case report. | brucellosis may be associated with a wide range of ophthalmic manifestations including endophthalmitis, which is a sight-threatening condition that needs to be rapidly recognized and treated to avoid permanent visual loss. a 26-year-old female with a 6-month history of vision loss in the left eye was treated with high dose systemic corticosteroids and azathioprine with an initial misdiagnosis elsewhere. a dense vitreous haze with opacities at the posterior hyaloid and a wide area of retinochoroi ... | 2017 | 28559723 |
| brucella melitensis prosthetic joint infection. | periprosthetic joint infection (pji) due to brucella spp. is rare. we report a case in a 75-year-old man and review 29 additional cases identified in a literature search. the diagnosis of brucella pji is challenging, in particular in non-endemic countries. serological tests prior to joint aspiration or surgical intervention are reasonable. involvement of infection control and timely information to laboratory personnel is mandatory upon diagnosis. there is no uniform treatment concept, neither wi ... | 2017 | 28540150 |
| transcriptional control of the lateral-flagellar genes of bradyrhizobium diazoefficiens. | bradyrhizobium diazoefficiens, a soybean n2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. we found that these genes are located in a single genomic cluster, organized in two monocistronic transcriptional units and three operons, one possibly containing an internal transcription start site. among the monocistronic ... | 2017 | 28533217 |
| evaluation of plasma sphingosine 1-phosphate, hepcidin and cardiovascular damage biomarkers (cardiac troponin i and homocysteine) in rats infected with brucellosis and vaccinated (rev-1, rb-51). | brucellosis is known as one of important zoonosis. studying the histological and biochemical effects of the disease could help to increase our knowledge about it. the aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of brucella (brucella melitensis and brucella abortus) and two vaccines (rev-1, rb-51) in the rat. forty male rats were divided into five groups (n = 8 in each group). two groups received suspensions of brucella abor ... | 2017 | 28533142 |
| molecular investigation of virulence factors of brucella melitensis and brucella abortus strains isolated from clinical and non-clinical samples. | brucella is zoonotic pathogen that induces abortion and sterility in domestic mammals and chronic infections in humans called malta fever. it is a facultative intracellular potential pathogen with high infectivity. the virulence of brucella is dependent upon its potential virulence factors such as enzymes and cell envelope associated virulence genes. the aim of this study was to investigate the brucella virulence factors among strains isolated from humans and animals in different parts of iran. ... | 2017 | 28506887 |
| characterization of novel omp31 antigenic epitopes of brucella melitensis by monoclonal antibodies. | brucellosis is a severe zoonotic disease worldwide. detection and identification of brucella species are essential to prevent or treat brucellosis in humans and animals. the outer membrane protein-31 (omp31) is a major protein of brucellae except for b. abortus, while the omp31 antigenic epitopes have not been extensively characterized yet. | 2017 | 28506316 |
| brucella melitensis m5-90δbp26 as a potential live vaccine that allows for the distinction between natural infection and immunization. | brucella is gram-negative intracellular bacterial pathogen that infects humans and animals and contributes to great economic losses in developing countries. presently, live attenuated brucella vaccines (brucella melitensis m5-90) are the most effective means of brucellosis control and prevention in animals. however, these vaccines have several drawbacks, such as an inability to distinguish between a natural infection and immunization and an association with abortions in pregnant animals. therefo ... | 2017 | 28482164 |
| a case report of pediatric brucellosis in an algerian immigrant. | an 8-year old girl presented to our facility with a 10-day history of fever, fatigue, abdominal pain and refusal to walk. she recently travelled from her native algeria where she first developed symptoms. on evaluation, she was ill-appearing, febrile and tachycardic with hepatosplenomegaly and lymphadenopathy noted on examination. a strong musty odor was also noted from the child. laboratory evaluation revealed pancytopenia, hyponatremia, and an elevated ast, alt, and ldh. malaria testing was ne ... | 2017 | 28480256 |
| high throughput virtual screening to identify novel natural product inhibitors for methionyltrna-synthetase of brucella melitensis. | the brucella melitensis methionyl-trna-synthetase (metrsbm) is a promising target for brucellosis drug development. the virtual screening of large libraries of a drug like molecules against a protein target is a common strategy used to identify novel inhibitors. a high throughput virtual screening was performed to identify hits to the potential antibrucellosis drug target, metrsbm. the best inhibitor identified from the literature survey was 1312, 1415, and 1430. in the virtual screening 56,400 ... | 2017 | 28479744 |
| cd14 gene silencing alters the microrna expression profile of raw264.7 cells stimulated by brucella melitensis infection. | innate recognition of brucella spp. is a key step in the activation of inflammation. cd14 binds pamps and is involved in lps-induced pro-inflammatory cytokine release. previously we showed that knock down of cd14 in raw264.7 macrophages disrupted brucella-host interactions. however, its effect on the macrophage microrna (mirna) expression profile, especially after stimulation by brucella infection, is still unclear. to identify mirnas involved in the macrophage response to brucella infection, we ... | 2017 | 28443393 |
| a novel pcr assay for detecting brucella abortus and brucella melitensis. | brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. the classical bacteriological detection process used to identify brucella spp. is difficult and time-consuming. this study aimed to develop a novel molecular assay for detecting brucellosis. | 2017 | 28443226 |
| protein/protein, dna/dna and dna/protein based vaccination strategies using truncated omp2b against brucella infection in balb/c mice. | the purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (tomp2b) from brucella abortus in balb/c mice. three immunization regimens protein/protein, dna/dna and dna/protein were used. immunization of mice with all vaccine strategies elicited a strong specific igg responses (igg2a titers over igg1) and provided t helper1 (th1) oriented immune responses. furthermore, protein/protein (pro/pro-) and dna/pro- vaccin ... | 2017 | 28391992 |
| rough brucella strain rm57 is attenuated and confers protection against brucella melitensis. | brucella melitensis (b. melitensis) is a facultative intracellular pathogen, which is the main epidemic strain in china. to overcome disadvantages of traditional live attenuated vaccines, in this study a rough mutant rm57 was induced from a b. melitensis isolate m1981. in order to uncover the reason of changes in the lps of rm57, the nucleotide sequences and transcription levels of all known genes related to lps synthesis were detected. as lps plays an important role in outer membrane integrity, ... | 2017 | 28390976 |
| whole genome sequencing of brucella melitensis isolated from 57 patients in germany reveals high diversity in strains from middle east. | brucellosis, a worldwide common bacterial zoonotic disease, has become quite rare in northern and western europe. however, since 2014 a significant increase of imported infections caused by brucella (b.) melitensis has been noticed in germany. patients predominantly originated from middle east including turkey and syria. these circumstances afforded an opportunity to gain insights into the population structure of brucella strains. brucella-isolates from 57 patients were recovered between january ... | 2017 | 28388689 |
| early animal farming and zoonotic disease dynamics: modelling brucellosis transmission in neolithic goat populations. | zoonotic pathogens are frequently hypothesized as emerging with the origins of farming, but evidence of this is elusive in the archaeological records. to explore the potential impact of animal domestication on zoonotic disease dynamics and human infection risk, we developed a model simulating the transmission of brucella melitensis within early domestic goat populations. the model was informed by archaeological data describing goat populations in neolithic settlements in the fertile crescent, an ... | 2017 | 28386446 |
| imported human brucellosis in belgium: bio and molecular typing of bacterial isolates, 1996-2015. | the aim of this study was to characterize by classical biotyping and multi-locus variable number tandem repeats (vntr) analysis (mlva) all brucella spp. derived from human cases in belgium from 1996 to 2015. final goals were to determine the species and biovar, to trace-back on genetic grounds the origin of each strain when patient history and risk factors were missing, and to survey for particular trends at the national level. | 2017 | 28384245 |
| omp31 plays an important role on outer membrane properties and intracellular survival of brucella melitensis in murine macrophages and hela cells. | brucellosis is an infectious disease that affects practically all species of mammals, including human, and is a major zoonosis worldwide. brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in phagocytic and nonphagocytic cells such as trophoblast and epithelial cells. among the six recognized species of the genus brucella, brucella melitensis is the main etiological agent involved in goat brucellosis and is also the most pathogenic for human. it c ... | 2017 | 28382472 |
| evaluation of a hypervariable octameric oligonucleotide fingerprints assay for identification of and discrimination between wild-type and vaccine strains of brucella melitensis. | objective to evaluate a hypervariable octameric oligonucleotide fingerprints (hoof-prints) assay for identification of and discrimination between wild-type and vaccine strains of brucella melitensis. sample brucella melitensis vaccine strain m5 and wild-type strain m43. procedures 8 pairs of primers (alterable, octameric nucleotides) were designed on the basis of a biological analysis of 8 flanking sequences in the dna of b melitensis. the hoof-prints technique was used to identify wild-type and ... | 2017 | 28345992 |
| serum biochemical profile and molecular detection of pathogens in semen of infertile male dromedary camels (camelus dromedarius). | detection of pathogens in the semen of camels has not been completely elucidated. therefore, the current study aimed to determine the association of some economically important pathogens with infertility in 94 male infertile camels through molecular detection and estimation of selected biochemical parameters in serum of these animals compared with a control non infected fertile animals (n=40). pcr analysis of semen samples of infertile camels indicated that, four potential pathogens namely mycop ... | 2017 | 28341394 |
| a novel real-time pcr assay for specific detection of brucella melitensis. | brucellosis is a zoonosis that occurs worldwide. the disease has been completely eradicated in livestock in sweden in 1994, and all cases of confirmed human brucellosis are imported into sweden from other countries. however, due to an increase in the number of refugees and asylum seekers from the middle-east to sweden, there is a need to improve the current diagnostic methodology for brucella melitensis. whilst culture of brucella species can be used as a diagnostic tool, real-time pcr approache ... | 2017 | 28340558 |
| molecular prevalence of putative virulence-associated genes in brucella melitensis and brucella abortus isolates from human and livestock specimens in iran. | molecular prevalence of nine putative virulence factors in two more prevalent brucella species in iranian patients and livestock was investigated. during five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of iran. all samples were cultured in blood culture media and subcultured into brucella agar medium. nine primer pairs were designed for detection of virb2, virb5, vcec, btpa, btpb, prpa, betb, bpe275 and bspb virulence factors using pcr and sequ ... | 2017 | 28284850 |
| brucella melitensis virb12 recombinant protein is a potential marker for serodiagnosis of human brucellosis. | the numerous drawbacks of current serological tests for diagnosis of brucellosis which mainly results from cross reactivity with lps from other gram-negative bacteria have generated an increasing interest to find more specific non-lps antigens. previous studies had indicated that brucella virb12 protein, a cell surface protein and component of type iv secretion system, induces antibody response during animal infection. however, this protein has not yet been tested as a serological diagnostic mar ... | 2017 | 28253917 |
| brucella melitensis as causative agent for neck abscess in an endemic area. | brucellosis, a zoonotic disease, is very common in the mediterranean basin and a major concern in livestock areas. we present a rare case of a brucella-caused abscess in the neck of a stock-breeder in an endemic greek area. | 2017 | 28251029 |
| serological survey of <i>brucella canis</i> in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 28235300 |
| brucella melitensis dacryoadenitis: a case report. | to present a case of brucellosis-related unilateral dacryoadenitis. | 2017 | 28226144 |
| genome sequences of human and livestock isolates of brucella melitensis and brucella abortus from the country of georgia. | brucellosis, which is among the most widespread global zoonotic diseases, is endemic in the nation of georgia and causes substantial human morbidity and economic loss. here, we report whole-genome sequences of three brucella melitensis and seven brucella abortus isolates from cattle, sheep, and humans that represent genetic groups discovered in georgia. | 2017 | 28183751 |
| mlva genotyping characteristics of human brucella melitensis isolated from ulanqab of inner mongolia, china. | brucellosis is a serious public health problem in ulanqab, which is a region located in the middle of the inner mongolia autonomous region adjacent to shanxi and hebei provinces. the disease is prevalent in both the latter provinces and ulanqab with the highest prevalence of brucellosis occurring in inner mongolia. the mlva-16 scheme is a genotyping tool for assessing genetic diversity and relationships among isolates. moreover, this genotyping tool can also be applied to epidemiological trace-b ... | 2017 | 28149294 |
| identification of otpr regulated srnas in brucella melitensis expressed under acidic stress and their roles in pathogenesis and metabolism. | small rnas (srnas) are the small regulatory molecules that regulate various biological processes in bacteria. though the functions of srnas are well documented, very little information is available on the srnas of brucella spp. the otpr is the response regulator of a two-component regulatory system, which plays a significant role in brucella virulence. in this study, we identified the srnas expressed in b. melitensis 16m and its otpr mutant under acidic stress from the rnaseq dataset. we identif ... | 2017 | 28131377 |
| molecular epidemiological investigation of brucella melitensis circulating in mongolia by mlva16. | mongolia has a high incidence of brucellosis in human and animals due to livestock husbandry. to investigate the genetic characteristics of mongolian b. melitensis, an mlva (multi-locus variable-number tandem-repeat analysis)-16 assay was performed with 94 b. melitensis isolates. they were identified as b. melitensis biovar (bv.) 1 (67), 3 (10) and rev. 1 vaccine strains (17) using a classical biotyping and multiplex pcr. in genotyping, three human isolates were grouped at 2 genotypes with sheep ... | 2017 | 28131373 |
| severe thrombocytopenic purpura in a child with brucellosis: case presentation and review of the literature. | brucellosis is still endemic and a significant public health problem in many mediterranean countries, including greece. it is a multisystemic disease with a broad spectrum of clinical manifestations including hematological disorders, such as anemia, pancytopenia, leucopenia, and thrombocytopenia. thrombocytopenia is usually moderate and attributed to bone marrow suppression or hypersplenism. rarely, autoimmune stimulation can cause severe thrombocytopenia with clinically significant hemorrhagic ... | 2017 | 28127481 |
| brucella abortus is prevalent in both humans and animals in bangladesh. | to determine the role of different brucella (b.) spp. in bangladesh, 62 animal samples and 500 human sera were tested. animal samples from cattle, goats and sheep (including milk, bull semen, vaginal swabs and placentas) were cultured for brucella spp. three test-positive human sera and all animal samples were screened by brucella genus-specific real-time pcr (rt-pcr), and positive samples were then tested by is711 rt-pcr to detect b. abortus and b. melitensis dna. only b. abortus dna was amplif ... | 2017 | 28068003 |
| extended multilocus sequence analysis to describe the global population structure of the genus brucella: phylogeography and relationship to biovars. | an extended multilocus sequence analysis (mlsa) scheme applicable to the brucella, an expanding genus that includes zoonotic pathogens that severely impact animal and human health across large parts of the globe, was developed. the scheme, which extends a previously described nine locus scheme by examining sequences at 21 independent genetic loci in order to increase discriminatory power, was applied to a globally and temporally diverse collection of over 500 isolates representing all 12 known b ... | 2016 | 28066370 |
| the bactec fx blood culture system detects brucella melitensis bacteremia in adult patients within the routine 1-week incubation period. | the performance of the bactec fx blood culture system for detecting brucella bacteremia within the routine 1-week incubation period was assessed in a prospective study conducted in an area in southern israel in which brucella melitensis is endemic. aerobic vials (bd bactec plus aerobic/f medium) inoculated with blood specimens obtained from adult patients with positive rose-bengal screening test results were monitored for 4 consecutive weeks, and blind subcultures of negative vials were performe ... | 2017 | 28053214 |
| control of animal brucellosis: the malaysian experience. | brucellosis is a zoonotic disease characterized by reproductive failure in animals and undulent fever in humans. in cattle, it is caused by brucella abortus while in goats by brucella melitensis, the main cause of brucellosis in humans. brucellosis in livestock has been associated with importation of animals from breeder herd of unknown disease status. the prevalence of bovine brucellosis brucella abortus in 2014 ranged between 1% and 2% in thailand and indonesia, and 4%-5% in malaysia and myanm ... | 2016 | 27955740 |
| comparison of potential protection conferred by three immunization strategies (protein/protein, dna/dna, and dna/protein) against brucella infection using omp2b in balb/c mice. | in the present study, immunogenicity and protective efficacy of the brucella outer membrane protein 2b (omp2b) was evaluated in balb/c mice using protein/protein, dna/dna and dna/protein vaccine strategies. immunization of mice with three vaccine regimens elicited a strong specific igg response (higher igg2a titers over igg1 titers) and provided th1-oriented immune response. vaccination of balb/c mice with the dna/pro regimen induced higher levels of ifn-γ/il-2 and conferred more protection leve ... | 2016 | 27938682 |
| first evaluation of an influenza viral vector based brucella abortus vaccine in sheep and goats: assessment of safety, immunogenicity and protective efficacy against brucella melitensis infection. | previously we developed and evaluated a candidate influenza viral vector based brucella abortus vaccine (flu-ba) administered with a potent adjuvant montanide gel01 in cattle, which was found safe and highly effective. this study was aimed to establish a proof-of-concept of the efficacy of flu-ba vaccine formulation in sheep and goats. we vaccinated sheep and goats with flu-ba vaccine and as a positive control vaccinated a group of animals with a commercial b. melitensis rev.1 vaccine. clinicall ... | 2016 | 27938677 |
| repetitive extragenic palindromic dna sequences from brucella melitensis stimulate toll-like receptor 9 signaling in macrophages. | brucella dna activates the host innate immune system via the intracellular toll-like receptor 9 (tlr9). however, the brucella dna sequences which are responsible for these immunostimulatory effects remain to be elucidated. the present study demonstrated that repetitive extragenic palindromic (reps) sequences present in brucella dna were able to stimulate macrophages through tlr9. the induction of interferon-α (ifn-α) production by brucella reps was detected in cultured raw264.7 mouse macrophages ... | 2017 | 27922679 |
| cloning, expression and molecular analysis of iranian brucella melitensis omp25 gene for designing a subunit vaccine. | brucellosis is a well-known domestic animal infectious disease, which is caused by brucella bacterium. the outer membrane protein 25 kda (omp25) gene plays an important role in simulating of tnf-α, ifn-α, macrophage, and cytokines cells. in the current study molecular cloning and expression analysis of omp25 gene for designing a subunit vaccine against brucella was investigated. amplifying the full length of candidate gene was performed using specific primers. sub-cloning of this gene conducted ... | 2016 | 27920824 |
| brucella melitensis 16m regulates the effect of air domain on inflammatory factors, autophagy, and apoptosis in mouse macrophage through the ros signaling pathway. | brucellosis is a highly contagious zoonosis caused by brucella. brucella can invade and persist inside host cells, which results in chronic infection. we constructed air interference and overexpression lentiviruses to acquire air interference, overexpression, and rescue stable expression cell lines. we also established a brucella melitensis 16m-infected macrophage model, which was treated with either the vehicle control or nac (ros scavenger n-acetylcysteine (nac) for 0, 3, 6, 12, and 24 h. conf ... | 2016 | 27907115 |
| an immunosensing system using stilbene glycoside as a fluorogenic substrate for an enzymatic reaction model. | a natural product, stilbene glycoside (2,3,5,4'-tetrahydroxydiphenylethylene-2-o-glucoside, tbg), has been evaluated for the first time as a potential substrate for horseradish peroxidase (hrp)-catalyzed fluorogenic reactions. the properties of tbg as a fluorogenic substrate for hrp and its application in a fluorometric enzyme-linked immunosensing system were compared with commercially available substrates such as p-hydroxyphenylpropionic acid (phppa), chavicol and amplex red using brucella meli ... | 2008 | 27873835 |
| brucellosis and thrombosis of the inferior vena cava. | we describe the case of a 23-year old woman with a newly diagnosed thrombosis of the inferior vena cava associated with a brucella melitensis infection. we suggest possible mechanisms leading to brucellosis-associated venous thrombosis and review 14 previously reported cases. . | 2017 | 27871206 |
| predictors of specific anti-brucella antibodies among humans in agro-pastoral communities in sengerema district, mwanza, tanzania: the need for public awareness. | human brucellosis remains to be a neglected zoonotic disease among agro-pastoral communities where livestock rearing is one of the main economic activities. this study was conducted in different agro-pastoral communities in sengerema district, mwanza, tanzania, to determine seroprevalence and predictors of anti-brucella antibodies, information that may influence public awareness on the risk factors and strategies to improve the diagnosis of brucellosis in developing countries. | 2016 | 27857610 |
| contamination of bovine, sheep and goat meat with brucella spp. | a study was conducted in order to evaluate the contamination by brucella spp. of meat from animals slaughtered because they had resulted positive for brucellosis at some time during their life. after slaughter and before delivery to market outlets, swab samples were taken from 307 carcasses of infected animals: 40 cattle, 60 sheep and 207 goats. the swabs were subsequently analysed by means of polymerase chain reaction (pcr) tests. in addition, bacteriological tests were carried out on the lymph ... | 2016 | 27853716 |
| draft genome sequences of five clinical strains of brucella melitensis isolated from patients residing in kuwait. | human brucellosis is a neglected and underrecognized infection of widespread geographic distribution. brucellosis is present on all inhabited continents and endemic in many areas of the world, including kuwait and the middle east. here, we present draft genome assemblies of five brucella melitensis strains isolated from brucellosis patients in kuwait. | 2016 | 27811090 |
| treatment of pulmonary brucellosis: a systematic review. | pulmonary involvement is a rare, focal complication of human brucellosis. the aim of this review is to describe clinical and radiologic features, treatment administered and clinical course of these patients. areas covered: we conducted a systematic search of scientific reports of brucellosis with pulmonary involvement published from january 1985 to july 2016. four main patterns of disease were observed: pneumonia, pleural effusion, nodules and interstitial pattern. cough and fever were the most ... | 2017 | 27790937 |
| isolation of a novel 'atypical' brucella strain from a bluespotted ribbontail ray (taeniura lymma). | a pleomorphic gram-negative, motile coccobacillus was isolated from the gills of a wild-caught bluespotted ribbontail ray after its sudden death during quarantine. strain 141012304 was observed to grow aerobically, to be clearly positive for cytochrome oxidase, catalase, urease and was initially identified as "brucella melitensis" or "ochrobactrum anthropi" by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and vitek2-compact(®), respectively. affiliation to the genu ... | 2017 | 27785661 |
| brucella abortus s19 vaccine protects dairy cattle against natural infection with brucella melitensis. | brucellosis is a zoonotic disease that can cause severe illness in humans and considerable economic loss in the livestock industry. although small ruminants are the preferential host for brucella melitensis, this pathogen has emerged as a cause for brucella outbreaks in cattle. s19 vaccination is implemented in many countries where b. abortus is endemic but its effectiveness against b. melitensis has not been validated. here we show that vaccine effectiveness in preventing disease transmission b ... | 2016 | 27771184 |
| the role of 'atypical' brucella in amphibians: are we facing novel emerging pathogens? | to discuss together the novel cases of brucella infections in frogs with the results of published reports to extend our current knowledge on 'atypical' brucellae isolated from amphibians and to discuss the challenges we face on this extraordinary emerging group of pathogens. | 2017 | 27740712 |
| implantable cardioverter device infection due to brucella melitensis in two patients. | 2016 | 27727012 | |
| human brucellosis in france in the 21st century: results from national surveillance 2004-2013. | brucellosis is a bacterial zoonotic disease mainly transmitted to humans by ruminants. in france, brucellosis has disappeared from ruminants herds. human brucellosis surveillance is performed through mandatory notification and the national reference center. | 2016 | 27717526 |
| omp31 of brucella melitensis 16m impairs the apoptosis of macrophages triggered by tnf-α. | outer membrane proteins (omps) of microorganisms play important roles in directly interacting with host cells. brucella species inhibit the apoptosis of host cells to benefit their own intracellular survival and replication. however, the association between omp31 of brucella and host cell apoptosis, and the underlying mechanism are unclear. in this study, an omp31 gene deletion mutant based on b. melitensis 16m was constructed. following the infection of raw264.7 cells with b. melitensis 16m or ... | 2016 | 27698784 |
| expression and regulation of the ery operon of brucella melitensis in human trophoblast cells. | brucellosis is primarily a disease of domestic animals in which the bacteria localizes to fetal tissues such as embryonic trophoblast cells and fluids containing erythritol, which stimulates brucella spp. growth. the utilization of erythritol is a characteristic of the genus brucella. the ery operon contains four genes (erya, eryb, eryc and eryd) for the utilization of erythritol, and plays a major role in the survival and multiplication of brucella spp. the objective of the present study was to ... | 2016 | 27698777 |
| susceptibility of select agents to predation by predatory bacteria. | select agents are microorganisms and toxins considered to be exploitable as biological weapons. although infections by many select agents can be treated by conventional antibiotics, the risk of an emerging or engineered drug resistant strain is of great concern. one group of microorganisms that is showing potential to control drug resistant gram-negative bacteria are the predatory bacteria from the genera bdellovibrio spp. and micavibrio spp. in this study, we have examined the ability of bdello ... | 2015 | 27682124 |
| dna-probe-target interaction based detection of brucella melitensis by using surface plasmon resonance. | surface plasmon resonance (spr) immunosensor using 4-mercaptobenzoic acid (4-mba) modified gold (4-mba/au) spr chip was developed first time for the detection of brucella melitensis (b. melitensis) based on the screening of its complementary dna target by using two different newly designed dna probes of is711 gene. herein, interaction between dna probes and target molecule are also investigated and result revealed that the interaction is spontaneous. the kinetics and thermodynamic results derive ... | 2017 | 27665519 |
| correction: brucella melitensis methionyl-trna-synthetase (metrs), a potential drug target for brucellosis. | [this corrects the article doi: 10.1371/journal.pone.0160350.]. | 2017 | 27658298 |
| human brucellosis in south africa: public health and diagnostic pitfalls. | human brucellosis in south africa (sa) is under-diagnosed and under-reported. this is because many clinicians have little or no experience in managing affected patients, and in part because of the nonspecific and insidious nature of the disease. a case of human brucellosis caused by brucella melitensis in a patient from the western cape province of sa is described, and the resulting exposure of staff members at two medical microbiology laboratories, as well as the public health investigation tha ... | 2016 | 27601111 |
| identification of brucella melitensis rev.1 vaccine-strain genetic markers: towards understanding the molecular mechanism behind virulence attenuation. | brucella melitensis rev.1 is an avirulent strain that is widely used as a live vaccine to control brucellosis in small ruminants. although an assembled draft version of rev.1 genome has been available since 2009, this genome has not been investigated to characterize this important vaccine. in the present work, we used the draft genome of rev.1 to perform a thorough genomic comparison and sequence analysis to identify and characterize the panel of its unique genetic markers. the draft genome of r ... | 2016 | 27595444 |
| immuno-pathophysiological responses of mouse model to experimental infection with brucella melitensis and its lipopolysaccharides via intraperitoneal route. | brucella melitensis is one of the major zoonotic pathogens with significant economic implications worldwide. the pathogenicity is complex and not always well understood. lipopolysaccharide (lps) remains the major virulent factor of b. melitensis and responsible for the mechanism by which the pathogen causes its deleterious effects. in this study, 84 mice of 6-8 weeks old of both sexes were divided equally into 3 groups; namely brucella melitensis infected group, lipopolysaccharide (lps) infected ... | 2016 | 27591112 |
| brucella melitensis prosthetic joint infection in a traveller returning to the uk from thailand: case report and review of the literature. | brucella spp. prosthetic joint infections are infrequently reported in the literature, particularly in returning travellers, and optimal treatment is unknown. | 2016 | 27591088 |
| prevalence of the most common virulence-associated genes among brucella melitensis isolates from human blood cultures in hamadan province, west of iran. | brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. despite animal vaccination, brucellosis remains endemic in some areas such as iran, especially in the western iranian province of hamadan. we sought to detect some of the most common virulence-associated genes in brucella isolated from human blood cultures to determine the prevalence of some virulence genes among brucella isolates. fifty-seven isolates were studied from patients with a clinical ... | 2016 | 27582592 |
| clinical and diagnostic aspects of brucellosis and antimicrobial susceptibility of brucella isolates in hamedan, iran. | current drug regimens for brucellosis are associated with relatively high rates of therapeutic failure or relapse. reduced antimicrobial susceptibility of brucella spp. has been proposed recently as a potential cause of therapeutic failure. the aim of this study was to evaluate the antibiotic resistance pattern of brucella melitensis clinical isolates by e-test method in hamadan, west of iran. in a 15-month period, all patients with suspected brucellosis were enrolled. blood specimens were colle ... | 2016 | 27580575 |
| endovascular management of concomitant thoracic and abdominal aortic ruptures resulting from brucellosis aortitis. | acute aortic symptomatology is an unusual manifestation of brucella melitensis infection. we present a rare case of acute multifocal thoracic and abdominal aortic ruptures arising from brucellosis aortitis managed exclusively with endovascular surgery. | 2017 | 27554701 |
| the abc transporter yejabef is required for resistance to antimicrobial peptides and the virulence of brucella melitensis. | the ability to resist the killing effects of host antimicrobial peptides (amps) plays a vital role in the virulence of pathogens. the brucella melitensis ni genome has a gene cluster that encodes abc transport. in this study, we constructed yeja1, yeja2, yejb, yeje, yejf, and whole yej operon deletion mutants, none of which exhibited discernible growth defect in tsb or minimal medium. unlike their parental strain, the mutants showed a significantly increased sensitivity to acidic stress. the niδ ... | 2016 | 27550726 |
| brucellosis in a refugee who migrated from syria to germany and lessons learnt, 2016. | a teenage woman migrating from syria arrived in may 2015 in germany. she gave birth to a healthy child in early 2016, but became febrile shortly after delivery. blood cultures revealed brucella melitensis. in retrospect, she reported contact with sheep in syria and recurrent pain in the hip joints over about five months before diagnosis of brucellosis. we discuss consequences for adequate treatment of mother and child as well as for clinical and laboratory management. | 2016 | 27525454 |
| genetic characterization and comparative genome analysis of brucella melitensis isolates from india. | brucellosis is the most frequent zoonotic disease worldwide, with over 500,000 new human infections every year. brucella melitensis, the most virulent species in humans, primarily affects goats and the zoonotic transmission occurs by ingestion of unpasteurized milk products or through direct contact with fetal tissues. brucellosis is endemic in india but no information is available on population structure and genetic diversity of brucella spp. in india. we performed multilocus sequence typing of ... | 2016 | 27525259 |
| genotyping of brucella melitensis and brucella abortus strains currently circulating in xinjiang, china. | brucellosis is a well-known zoonotic disease that can cause severe economic and healthcare losses. xinjiang, one of the biggest livestock husbandry sectors in china, has gone through increasing incidence of brucellosis in cattle and small ruminants recently. in this paper, 50 b. melitensis strains and 9 b. abortus strains collected from across xinjiang area (from 2010 to 2015) were genotyped using multiple locus variable-number tandem-repeat (vntr) analysis (mlva) and multi-locus sequence typing ... | 2016 | 27521159 |
| antimicrobial susceptibility testing of brucella melitensis isolated from patients with acute brucellosis in a centre of iran. | structured to purpose: human brucellosis is one of the most common zoonotic infections worldwide, which remains one of the major problems for public health. despite the world health organization's recommendation for human brucellosis treatment, sporadic cases of relapse have been reported. the aim of this study was to assess the susceptibility of brucella isolates to common antibiotics that are prescribed by the physician for the treatment of brucellosis and also to determine the minimum inhibit ... | 2017 | 27514957 |
| brucella melitensis methionyl-trna-synthetase (metrs), a potential drug target for brucellosis. | we investigated brucella melitensis methionyl-trna-synthetase (bmmetrs) with molecular, structural and phenotypic methods to learn if bmmetrs is a promising target for brucellosis drug development. recombinant bmmetrs was expressed, purified from wild type brucella melitensis biovar abortus 2308 strain atcc/crp #dd-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-trna-synthetase inhibitors of the blood stage form of trypanosoma bru ... | 2016 | 27500735 |
| comparing rapid and specific detection of brucella in clinical samples by pcr-elisa and multiplex-pcr method. | rapid diagnosis and differentiation of brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. this study aimed to identify and compare pcr-elisa as a more accurate diagnositc test with other common molecular and serological tests. | 2016 | 27499776 |
| immune response induced by conjunctival immunization with polymeric antigen blsomp31 using a thermoresponsive and mucoadhesive in situ gel as vaccine delivery system for prevention of ovine brucellosis. | control of ovine brucellosis with subcellular vaccines can solve some drawbacks associated with the use of brucella melitensis rev.1. previous studies have demonstrated that the polymeric antigen blsomp31 administered by parenteral route was immunogenic and conferred significant protection against b. ovis in rams. immunization with blsomp31 by conjunctival route could be efficient for the induction of mucosal and systemic immune responses. in this work, we evaluated the conjunctival immunization ... | 2016 | 27496742 |
| development and application of dot-enzyme-linked immunosorbent (dot-elisa) assay for detection of brucella melitensis and evaluation of the shedding pattern in infected goats. | early and accurate diagnosis of brucella melitensis is essential for the treatment and control of brucellosis both in animals and humans. the thrust for the development of a rapid diagnostic technique to overcome the limitations of conventional microbiological and serological tests brought about this investigation on the development and application of dot-elisa for antigen and antibody detection in infected goats. fifteen apparently healthy boer aged 2-3 years which tested negative for brucellos ... | 2017 | 27494045 |
| isolation & characterization of brucella melitensis isolated from patients suspected for human brucellosis in india. | brucellosis is endemic in the southern part of india. a combination of biochemical, serological and molecular methods is required for identification and biotyping of brucella. the present study describes the isolation and biochemical, molecular characterization of brucella melitensis from patients suspected for human brucellosis. | 2016 | 27488010 |
| biocidal and sporicidal efficacy of pathoster(®) 0.35% and pathoster(®) 0.50% against bacterial agents in potential bioterrorism use. | the use of products that can neutralize or significantly reduce the microbial load and that are not harmful to human health and the environment represents a milestone in the fight against the spread of infectious diseases. peracetic acid, besides being an excellent sterilizing and sporicidal agent, is harmless to humans and the environment when it is used in a common dosage. however, the high costs and loss of efficacy of the product very quickly after its reconstitution limit its use. we evalua ... | 2016 | 27482880 |
| an nmr-guided screening method for selective fragment docking and synthesis of a warhead inhibitor. | selective hits for the glutaredoxin ortholog of brucella melitensis are determined using std nmr and verified by trnoe and (15)n-hsqc titration. the most promising hit, rk207, was docked into the target molecule using a scoring function to compare simulated poses to experimental data. after elucidating possible poses, the hit was further optimized into the lead compound by extension with an electrophilic acrylamide warhead. we believe that focusing on selectivity in this early stage of drug disc ... | 2016 | 27438815 |
| activation of bovine neutrophils by brucella spp. | brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus brucella. while many species of brucella exist, brucella melitensis, brucella abortus, and brucella suis are the most common pathogens of humans and livestock. the virulence of brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. the aim of this study was to characterize the bo ... | 2016 | 27436438 |
| an analysis of children with brucellosis associated with haemophagocytic lymphohistiocytosis. | this retrospective study included seven paediatric cases aged from 4 to 14 (10.2±3.4) years with pathologically proved haemophagocytic lymphohistiocytosis from a single institution during 2009 and 2013. over this time period, 496 patients with brucellosis were diagnosed. none of the patients (3 boys and 4 girls) had a history of any haematologic disorder. all patients had an anamnesis for recently consumed unpasteurised homemade dairy products or had a contact history with sheep and/or cows. the ... | 2016 | 27367321 |
| brucella tir-like protein tcpb/btp1 specifically targets the host adaptor protein mal/tirap to promote infection. | brucella spp. are known to avoid host immune recognition and weaken the immune response to infection. brucella like accomplish this by employing two clever strategies, called the stealth strategy and hijacking strategy. the tir domain-containing protein (tcpb/btp1) of brucella melitensis is thought to be involved in inhibiting host nf-κb activation by binding to adaptors downstream of toll-like receptors. however, of the five tir domain-containing adaptors conserved in mammals, whether myd88 or ... | 2016 | 27311859 |
| omics of brucella: species-specific srna-mediated gene ontology regulatory networks identified by computational biology. | brucella is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. brucella species are currently intensively studied with a view to developing novel global health diagnostics and therapeutics. in this context, small rnas (srnas) are one of the emerging topical areas; they play significant roles in regulating gene expression and cellular processes in bacteria. in the present study, we forecast srnas in three brucella species that infect humans, namely brucella melit ... | 2016 | 27223678 |
| [brucella melitensis (bm) bacteremia in hospitalized adult patients in southern israel]. | southern israel is endemic for brucella melitensis (bm) infection. | 2016 | 27215118 |
| latex agglutination using the periplasmic proteins antigen of brucella melitensis is a successful, rapid, and specific serodiagnostic test for ovine brucellosis. | brucellosis, especially caused by brucella melitensis, is considered the most-widespread zoonosis in the world, particularly in developing countries. this study was planned to develop an accurate test for diagnosis of ovine brucellosis using a specific hot saline extracted soluble brucella melitensis periplasmic proteins (sbpps). the efficacy of the latex agglutination test (lat) using sbpps compared to the rose bengal test (rbt), buffered plate agglutination test (bpat), serum agglutination tes ... | 2016 | 27207442 |
| [a case of brucellosis with intermittent fever in a patient returning from france]. | we herein report on a 62-year-old man who presented with symptoms of intermittent fever that persisted after returning from a trip to france. during his trip, he had eaten natural cheese. although no bacteria could be isolated from blood culture, the serum agglutination test showed a positive antibody titer of 1 : 160 for brucella canis. the patient responded well to combination antibiotic therapy consisting of gentamicin, rifampicin, and doxycycline, and his symptoms improved. he became antibod ... | 2016 | 27197442 |
| cases of human brucellosis in sweden linked to middle east and africa. | human brucellosis cases are still reported each year in sweden despite eradication of the disease in animals. epidemiological investigation has never been conducted to trace back the source of human infection in the country. the purpose of the study was to identify the source of infection for 16 human brucellosis cases that occurred in sweden, during the period 2008-2012. | 2016 | 27188619 |
| brucella melitensis and mycobacterium tuberculosis depict overlapping gene expression patterns induced in infected thp-1 macrophages. | pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts' defensive mechanisms. in this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their host's killing attitude. studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. brucellosis, caused by the brucella strains, is a zoonotic bacterial disea ... | 2015 | 27175205 |
| comprehensive identification of immunodominant proteins of brucella abortus and brucella melitensis using antibodies in the sera from naturally infected hosts. | brucellosis is a debilitating zoonotic disease that affects humans and animals. the diagnosis of brucellosis is challenging, as accurate species level identification is not possible with any of the currently available serology-based diagnostic methods. the present study aimed at identifying brucella (b.) species-specific proteins from the closely related species b. abortus and b. melitensis using sera collected from naturally infected host species. unlike earlier reported investigations with eit ... | 2016 | 27144565 |
| first case of human brucellosis caused by brucella melitensis in korea. | 2016 | 27139618 |