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reduced th22 cell proportion and prevention of atopic dermatitis in infants following maternal probiotic supplementation.in the randomized, controlled study probiotics in the prevention of allergy among children in trondheim (propact), maternal probiotic supplementation reduced the incidence of atopic dermatitis (ad) in the offspring. in the current study, we hypothesized that the effect was mediated by a shift in the t helper (th) cells in the children.201728346719
effect of probiotic on innate inflammatory response and viral shedding in experimental rhinovirus infection - a randomised controlled trial.ingestion of probiotics appears to have modest effects on the incidence of viral respiratory infection. the mechanism of these effects is not clear; however, there is evidence from animal models that the probiotic may have an effect on innate immune responses to pathogens. the purpose of this randomised, placebo-controlled study was to determine the effect of administration of bifidobacterium animalis subspecies lactis bl-04 on innate and adaptive host responses to experimental rhinovirus challe ...201728343401
selective method for identification and quantification of bifidobacterium animalis subspecies lactis bb-12 (bb-12) from the gastrointestinal tract of healthy volunteers ingesting a combination probiotic of bb-12 and lactobacillus rhamnosus gg.to develop a novel validated method for the isolation of bifidobacterium animalis ssp. lactis bb-12 (bb-12) from faecal specimens and apply it to studies of bb-12 and lactobacillus rhamnosus gg (lgg) recovered from the healthy human gastrointestinal (gi) tract.201728256070
a proliferative probiotic bifidobacterium strain in the gut ameliorates progression of metabolic disorders via microbiota modulation and acetate elevation.the gut microbiota is an important contributor to the worldwide prevalence of metabolic syndrome (ms), which includes obesity and diabetes. the anti-ms effects exerted by bifidobacterium animalis ssp. lactis gcl2505 (blag), a highly proliferative bifidobacterium strain in the gut, and b. longum ssp. longum jcm1217(t) (bloj) were comparatively examined. blag treatment reduced visceral fat accumulation and improved glucose tolerance, whereas bloj had no effect on these parameters. gut microbial an ...201728252037
the effect of bifidobacterium animalis ssp. lactis hn019 on cellular immune function in healthy elderly subjects: systematic review and meta-analysis.elderly people have increased susceptibility to infections and cancer that are associated with decline in cellular immune function. the objective of this work was to determine the efficacy of bifidobacterium (b.) animalis ssp. lactis hn019 (hn019) supplementation on cellular immune activity in healthy elderly subjects. we conducted a systematic review of medline and embase for controlled trials that reported polymorphonuclear (pmn) cell phagocytic capacity or natural killer (nk) cell tumoricidal ...201728245559
spray-drying process preserves the protective capacity of a breast milk-derived bifidobacterium lactis strain on acute and chronic colitis in mice.gut microbiota dysbiosis plays a central role in the development and perpetuation of chronic inflammation in inflammatory bowel disease (ibd) and therefore is key target for interventions with high quality and functional probiotics. the local production of stable probiotic formulations at limited cost is considered an advantage as it reduces transportation cost and time, thereby increasing the effective period at the consumer side. in the present study, we compared the anti-inflammatory capaciti ...201728233848
safety of bifidobacterium animalis subsp. lactis (b. lactis) strain bb-12-supplemented yogurt in healthy children.probiotics are live microorganisms that may provide health benefits to the individual when consumed in sufficient quantities. for studies conducted on health or disease endpoints on probiotics in the united states, the food and administration has required those studies to be conducted as investigational new drugs. this phase i, double-blinded, randomized, controlled safety study represents the first requirement of this pathway. the purpose of the study was to determine the safety of bifidobacter ...201728114246
diversity of the subspecies bifidobacterium animalis subsp. lactis.strains of bifidobacterium animalis subsp. lactis are well-known health-promoting probiotics used commercially. b. animalis subsp. lactis has been isolated from different sources, and little is known about animal isolates of this taxon. the aim of this study was to examine the genotypic and phenotypic diversity between b. animalis subsp. lactis strains different animal hosts including cameroon sheep, barbary sheep, okapi, mouflon, german shepard) and to compare to bb12, food isolates and the col ...201728108391
early benefits of a starter formula enriched in prebiotics and probiotics on the gut microbiota of healthy infants born to hiv+ mothers: a randomized double-blind controlled trial.the gut microbiota of infants is shaped by both the mode of delivery and the type of feeding. the gut of vaginally and cesarean-delivered infants is colonized at different rates and with different bacterial species, leading to differences in the gut microbial composition, which may persist up to 6 months. in a multicenter, randomized, controlled, double-blind trial conducted in south africa, we tested the effect of a formula supplemented with a prebiotic (a mixture of bovine milk-derived oligosa ...201628096702
effect of probiotics on diarrhea in children with severe acute malnutrition: a randomized controlled study in uganda.the aim of the study was to assess the effect of probiotics on diarrhea during in- and outpatient treatment of children with severe acute malnutrition (sam).201728079729
fermented probiotic beverages based on acid whey.production of fermented probiotic beverages can be a good method for acid whey usage. the obtained products combine a high nutritional value of whey with health benefits claimed for probiotic bacteria. the aim of the study was to define quality properties of beverages based on fresh acid whey and milk with addition of buttermilk powder or sweet whey powder.201728068045
effects of bifidobacterium animalis lactis hn019 (dr10tm), inulin, and micronutrient fortified milk on faecal dr10tm, immune markers, and maternal micronutrients among indonesian pregnant women.maternal nutrition affects fetal growth and development. this study evaluates the effects of milk powder fortified with micronutrients, docosahexaenoic acid (dha), a prebiotic, and probiotic bifidobacterium animalis subsp. lactis hn019 dr10tm on the micronutrient status, as well as the presence of faecal probiotic and immune markers in pregnant women.201628027638
comparative analysis of the rats' gut microbiota composition in animals with different ginsenosides metabolizing activity.following oral intake of panax ginseng, major ginsenosides are metabolized to deglycosylated ginsenosides by gut microbiota before absorption into the blood. as the composition of gut microbiota varies between individuals, metabolic activities are significantly different. we selected 6 rats with low efficiency metabolism (lem) and 6 rats with high efficiency metabolism (hem) from 60 rats following oral administration of panax ginseng extract, and analyzed their gut microbiota composition using i ...201728025886
use of probiotics as an adjuvant to sequential h. pylori eradication therapy: impact on eradication rates, treatment resistance, treatment-related side effects, and patient compliance.to evaluate the effect of probiotics administered as an adjuvant to sequential helicobacter pylori (h. pylori) eradication therapy on treatment outcome and patient compliance.201728007678
starter formula enriched in prebiotics and probiotics ensures normal growth of infants and promotes gut health: a randomized clinical trial.prebiotics and probiotics exert beneficial effects by modulating gut microbiota and immune system. this study evaluates efficacy and safety of an infant formula containing bovine milk-derived oligosaccharides and bifidobacterium animalis ssp lactis (b. lactis) (cncm i-3446) on incidence of diarrhea and febrile infections during the first year of life (primary outcome).201728002391
characterization of cinnamoyl esterases from different lactobacilli and bifidobacteria.a high variety of plants that are used for food production contain esterified hydroxycinnamic acids. as their free forms display several benefits, like an enhanced absorption in human intestinal tract, anti-oxidative and anti-carcinogenic effects, an improved protein solubility and reduced discoloration, the microbial ability to cleave the ester bond is highly desired. in order to examine potential fermentation strains for this purpose, six different lactic acid bacteria and one bifidobacterial ...201727999938
integrated role of bifidobacterium animalis subsp. lactis supplementation in gut microbiota, immunity, and metabolism of infant rhesus monkeys.to investigate the impact of probiotic supplementation of infant formula on immune parameters, intestinal microbiota, and metabolism, five individually housed infant rhesus monkeys exclusively fed standard infant formula supplemented with probiotics (bifidobacterium animalis subsp. lactis hn019) from birth until 3 months of age were compared with five standard formula-fed and five breast-fed monkeys. anthropometric measurements, serum insulin, immune parameters, fecal microbiota, and metabolic p ...201727921083
an exploratory investigation of endotoxin levels in novice long distance triathletes, and the effects of a multi-strain probiotic/prebiotic, antioxidant intervention.gastrointestinal (gi) ischemia during exercise is associated with luminal permeability and increased systemic lipopolysaccharides (lps). this study aimed to assess the impact of a multistrain pro/prebiotic/antioxidant intervention on endotoxin unit levels and gi permeability in recreational athletes. thirty healthy participants (25 males, 5 females) were randomly assigned either a multistrain pro/prebiotic/antioxidant (lab⁴anti; 30 billion cfu·day(-1) containing 10 billion cfu·day(-1)lactobacill ...201627869661
effect of bifidobacterium animalis ssp. lactis gcl2505 on visceral fat accumulation in healthy japanese adults: a randomized controlled trial.bifidobacterium animalis ssp. lactis gcl2505 (b. lactis gcl2505) is able to survive passage through the intestine and then proliferate, leading to an increase in the amount of gut bifidobacteria. in the present study, we evaluated the impact of b. lactis gcl2505 on abdominal visceral fat storage in overweight and mildly obese japanese adults. this clinical study was a double-blind, randomized, placebo-controlled, parallel-group comparative trial performed for 12 weeks. healthy japanese subjects ...201627867803
in vitro fermentation of different fructo-oligosaccharides by bifidobacterium strains for the selection of synbiotic combinations.the use of selected probiotics, prebiotics and/or synbiotics, constitute an interesting dietary strategy for intestinal microbiota modulation in case of dysbiosis. species of the genus bifidobacterium are among the most currently used probiotics for human consumption since they have shown beneficial effects in the prevention and treatment of some disorders. bifidobacteria are saccharolytic microorganisms, but their ability to use different carbohydrates varies among strains. in this study, we in ...201727866040
genotyping by pcr and high-throughput sequencing of commercial probiotic products reveals composition biases.recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. although probiotics have historically been defined as microorganisms that convey health benefits when ingested in sufficient viable amounts, this description now includes the stipulation "well defined strains," encompassing definitive taxonomy for consumer consideration and regulatory oversight. here, we evaluated 52 commercial dietary supplements ...201627857709
orally administered multispecies probiotic formulations to prevent uro-genital infections: a randomized placebo-controlled pilot study.the aim of this study was to evaluate in the vagina of 60 pre-menopausal women the detection of orally administered multispecies probiotic formulations showing anti-microbial properties in test in vitro.201727826653
effect of bifidobacterium animalis subsp. lactis gcl2505 on the physiological function of intestine in a rat model.bifidobacterium animalis ssp. lactis gcl2505 has been shown to proliferate in the human intestine. the intestinal dynamics and physiological effects of gcl2505 as well as the mechanism underlying proliferation in the gut were investigated. gcl2505 showed markedly higher resistance to free bile acids (cholic and deoxycholic acids) than other bifidobacterial species. the intestinal dynamics of gcl2505 and b. longum ssp. longum jcm1217(t) was compared. the level of b. animalis ssp. lactis in the gc ...201627826427
in vitro inhibition of eimeria tenella sporozoite invasion into host cells by probiotics.the aim was to study the effects of probiotics isolated from the intestinal tract of livestock animals on eimeria tenella invasion into madin-darby bovine kidney (mdbk) cells in vitro. e. tenella sporozoites were purified and labeled with 5(6)-carboxyfluorescein diacetate n-succinimidyl ester before seeding on cell cultures, and invasion was evaluated by fluorescence microscopy. two protocols (a and b) were used. in protocol a, enterococcus faecium # 589 or lactobacillus salivarius subsp. saliva ...201627809987
modulation of the bifidobacterial communities of the dog microbiota by zeolite.during last decades canine health and well being is becoming an important issue for human owners. in dogs, several factors including diet, pathogenic bacterial and stress conditions can affect the composition of the gut microbiota. in this study, we evaluated the effect of dietary chabazitic zeolitite (cz) supplementation on the contribution of bifidobacteria to the fecal microbiota in training hunting dogs. fecal microbiota cataloging based on 16s rrna microbial profiling analyses highlighted a ...201627713735
influence of the addition of lactobacillus acidophilus la-05, bifidobacterium animalis subsp. lactis bb-12 and inulin on the technological, physicochemical, microbiological and sensory features of creamy goat cheese.the effects of the addition of lactobacillus acidophilus la-05, bifidobacterium animalis subsp. lactis bb-12 and inulin on the quality characteristics of creamy goat cheese during refrigerated storage were evaluated. the manufactured cheeses included the addition of starter culture (lactococcus lactis subsp. lactis and lactococcus lactis subsp. cremoris - r-704) (cc); starter culture, l. acidophilus la-05 and inulin (cla); starter culture, b. lactis bb-12 and inulin (cbb); or starter culture, l. ...201627711907
the science behind the probiotic strain bifidobacterium animalis subsp. lactis bb-12(®).this review presents selected data on the probiotic strain bifidobacterium animalis subsp. lactis bb-12(®) (bb-12(®)), which is the world's most documented probiotic bifidobacterium. it is described in more than 300 scientific publications out of which more than 130 are publications of human clinical studies. the complete genome sequence of bb-12(®) has been determined and published. bb-12(®) originates from chr. hansen's collection of dairy cultures and has high stability in foods and as freeze ...201427682233
the different effects of probiotics treatment on salmonella-induced interleukin-8 response in intestinal epithelia cells via pi3k/akt and nod2 expression.salmonella spp. remains a major public health problem for the whole world. intestinal epithelial cells serve as an essential component of the innate mucosal immune system to defend against salmonella infection. a substantial amount of evidence has accumulated that probiotics can regulate interleukin 8 (il-8) involved in innate immunity. however, the exact effect of probiotics on epithelial il-8 response to salmonella infection is not well understood. therefore, we investigated the action of prob ...201627680208
benefits of bifidobacterium animalis subsp. lactis probiotic in experimental periodontitis.this study evaluates effects of topical administration of probiotic bacteria of the genus bifidobacterium on experimental periodontitis (ep) in rats.201727660886
impact of gluten-friendly bread on the metabolism and function of in vitro gut microbiota in healthy human and coeliac subjects.the main aim of this paper was to assess the in vitro response of healthy and coeliac human faecal microbiota to gluten-friendly bread (gfb). thus, gfb and control bread (cb) were fermented with faecal microbiota in ph-controlled batch cultures. the effects on the major groups of microbiota were monitored over 48 h incubations by fluorescence in situ hybridisation. short-chain fatty acids (scfas) were measured by high-performance liquid chromatography (hplc). furthermore, the death kinetics of l ...201627632361
chemical and structural characterization of pholiota nameko extracts with biological properties.edible mushrooms including pholiota nameko are excellent sources of extractable bioactive compounds with much to explore. enzymatic extractions with cellulase and viscozyme were responsible for highest extraction yields (67-77%). no strong antioxidant activity was observed although extracts were able to scavenge abts(+) and oh(+). potential prebiotic activity was observed in all extracts, some increasing 1.4-2 log cycles of lactobacillus acidophilus la-5 and bifidobacterium animalis bb12. 30-50% ...201727596407
a peptidome-based phylogeny pipeline reveals differential peptides at the strain level within bifidobacterium animalis subsp. lactis.bifidobacteria are gut commensal microorganisms belonging to the actinobacteria group. some specific strains of bifidobacterium animalis subsp. lactis are used in functional foods as they are able to exert health-promoting effects in the human host. due to the limited genetic variability within this subspecies, it is sometimes difficult for a manufacturer to properly track its strain once included in dairy products or functional foods. in this paper, we present a peptidome-based analysis in whic ...201627554155
systematic review and meta-analysis: multi-strain probiotics as adjunct therapy for helicobacter pylori eradication and prevention of adverse events.eradication rates with triple therapy for helicobacter pylori infections have currently declined to unacceptable levels worldwide. newer quadruple therapies are burdened with a high rate of adverse events. whether multi-strain probiotics can improve eradication rates or diminish adverse events remains uncertain.201627536365
an atp binding cassette transporter mediates the uptake of α-(1,6)-linked dietary oligosaccharides in bifidobacterium and correlates with competitive growth on these substrates.the molecular details and impact of oligosaccharide uptake by distinct human gut microbiota (hgm) are currently not well understood. non-digestible dietary galacto- and gluco-α-(1,6)-oligosaccharides from legumes and starch, respectively, are preferentially fermented by mainly bifidobacteria and lactobacilli in the human gut. here we show that the solute binding protein (blg16bp) associated with an atp binding cassette (abc) transporter from the probiotic bifidobacterium animalis subsp. lactis b ...201627502277
screening of cholesterol-lowering bifidobacterium from guizhou xiang pigs, and evaluation of its tolerance to oxygen, acid, and bile.cardiovascular and cerebrovascular diseases seriously harm human health, and bifidobacterium is the most beneficial probiotic in the gastrointestinal tract of humans. this work aimed to screen cholesterol-lowering bifidobacterium from guizhou xiang pig and evaluate its tolerance to oxygen, acid, and bile. twenty-seven aerotolerant strains with similar colony to bifidobacterium were isolated through incubation at 37℃ in 20% (v/v) co2-80% (v/v) atmospheric air by using mupirocin lithium modified m ...201627499662
comparative in vitro investigation of the cariogenic potential of bifidobacteria.this study aimed to assess the in vitro cariogenic potential of some bifidobacterium species in comparison with caries-associated bacteria.201627475723
bacteroides fragilis metabolises exopolysaccharides produced by bifidobacteria.bacteroides fragilis is the most frequent species at the human intestinal mucosal surface, it contributes to the maturation of the immune system although is also considered as an opportunistic pathogen. some bifidobacterium strains produce exopolysaccharides (eps), complex carbohydrate polymers that promote changes in the metabolism of b. fragilis when this microorganism grows in their presence. to demonstrate that b. fragilis can use eps from bifidobacteria as fermentable substrates, purified e ...201627418149
in vitro gastrointestinal resistance of lactobacillus acidophilus la-5 and bifidobacterium animalis bb-12 in soy and/or milk-based synbiotic apple ice creams.the viability and resistance to simulated gastrointestinal (gi) conditions of lactobacillus acidophilus la-5 and bifidobacterium animalis bb-12 in synbiotic ice creams, in which milk was replaced by soy extract and/or whey protein isolate (wpi) with inulin, were investigated. the ice creams were showed to be satisfactory vehicles for la-5 and bb-12 (populations around 7.5logcfu/g), even after the whole storage period (84days/-18°c). in all formulations, the propidium monoazide qpcr (pma-qpcr) an ...201627387254
effect of a ropy exopolysaccharide-producing bifidobacterium animalis subsp. lactis strain orally administered on dss-induced colitis mice model.exopolysaccharide (eps)-producing bifidobacteria, particularly bifidobacterium animalis subsp. lactis strains, are used in the functional food industry as promising probiotics with purported beneficial effects. we used three isogenic strains of b. animalis subsp. lactis, with different eps producing phenotypes (mucoid-ropy and non-ropy), in order to determine their capability to survive the murine gastrointestinal tract transit, as well as to evaluate their role in improving clinical outcomes in ...201627375589
effects of the food manufacturing chain on the viability and functionality of bifidobacterium animalis through simulated gastrointestinal conditions.the viability and functionality of probiotics may be influenced by industrial production processes resulting in a decrease in probiotic efficiency that benefit the health of humans. this study aimed to investigate the probiotic characteristics of bifidobacterium strains isolated from fecal samples of healthy thai infants. in the present work, three local strains (bf014, bf052, and bh053) belonging to bifidobacterium animalis showed a great resistance against conditions simulating the gastrointes ...201627333286
effect of bifidobacterium upon clostridium difficile growth and toxicity when co-cultured in different prebiotic substrates.the intestinal overgrowth of clostridium difficile, often after disturbance of the gut microbiota by antibiotic treatment, leads to c. difficile infection (cdi) which manifestation ranges from mild diarrhea to life-threatening conditions. the increasing cdi incidence, not only in compromised subjects but also in traditionally considered low-risk populations, together with the frequent relapses of the disease, has attracted the interest for prevention/therapeutic options. among these, probiotics, ...201627242753
bifidobacterium animalis ssp. lactis cncm-i2494 restores gut barrier permeability in chronically low-grade inflamed mice.growing evidence supports the efficacy of many probiotic strains in the management of gastrointestinal disorders associated with deregulated intestinal barrier function and/or structure. in particular, bifidobacteria have been studied for their efficacy to both prevent and treat a broad spectrum of animal and/or human gut disorders. the aim of the current work was thus to evaluate effects on intestinal barrier function of bifidobacterium animalis ssp. lactis cncm-i2494, a strain used in fermente ...201627199937
effects of synbiotic fermented milk containing lactobacillus acidophilus la-5 and bifidobacterium animalis ssp. lactis bb-12 on the fecal microbiota of adults with irritable bowel syndrome: a randomized double-blind, placebo-controlled trial.we conducted a randomized double-blind, placebo-controlled multicentric study to investigate the influence of a synbiotic fermented milk on the fecal microbiota composition of 30 adults with irritable bowel syndrome (ibs). the synbiotic product contained lactobacillus acidophilus la-5, bifidobacterium animalis ssp. lactis bb-12, streptococcus thermophilus, and dietary fiber (90% inulin, 10% oligofructose), and a heat-treated fermented milk without probiotic bacteria or dietary fiber served as pl ...201627157575
identification of surface-associated proteins of bifidobacterium animalis ssp. lactis klds 2.0603 by enzymatic shaving.bifidobacteria are commensal microorganisms of the human and animal intestinal tract, and their surface proteins can mediate bacterial communication and chemical sensing in the environment, as well as facilitate interactions between bacteria and the host. however, a systematic study of the outer surface-associated proteome of bifidobacteria has not been undertaken. in the present study, the proteins located on the surface of bifidobacterium animalis ssp. lactis klds 2.0603 were systematically id ...201627132091
a prebiotic mixture improved lactobacillus acidophilus and bifidobacterium animalis gastrointestinal in vitro resistance in petit-suisse.the survival of two probiotic strains -lactobacillus acidophilus la-5 and bifidobacterium animalis bb-12 - incorporated into probiotic (pc) and into synbiotic (sc, with inulin + fructooligosaccharides, respectively, at 7.5 and at 2.5 g per 100 g) petit-suisse cheese was investigated in the beginning (day 1) and at the end (28 days) of storage at 4 °c when the food products were subjected to in vitro gastrointestinal simulated assays. species-specific quantitative real time pcr (qpcr) combined wi ...201627112363
oligosaccharides released from milk glycoproteins are selective growth substrates for infant-associated bifidobacteria.milk, in addition to nourishing the neonate, provides a range of complex glycans whose construction ensures a specific enrichment of key members of the gut microbiota in the nursing infant, a consortium known as the milk-oriented microbiome. milk glycoproteins are thought to function similarly, as specific growth substrates for bifidobacteria common to the breast-fed infant gut. recently, a cell wall-associated endo-β-n-acetylglucosaminidase (endobi-1) found in various infant-borne bifidobacteri ...201627084007
influence of 6 different intestinal bacteria on beltsville small white turkey semen.the turkey industry relies totally on artificial insemination to continue and improve production. if something compromises the insemination process, such as contaminated semen, it becomes a detrimental loss to the industry. bacteria have been found in broiler breeder males to reduce sperm motility. the sperm quality index (sqi) is a quick method to determine avian sperm motility using the sperm quality analyzer (sqa). therefore, the objective of this study was to determine if bacteria have an ef ...201627081196
cd19 lymphocyte proliferation induced by bifidobacterium animalis subsp. lactis in c57bl/6 mice experimentally infected with toxoplasma gondii.toxoplasmosis is frequently acquired through the oral route by the ingestion of cysts or oocysts of toxoplasma gondii. once ingested, the parasites penetrate the intestinal epithelial cells and rapidly disseminate to all organs in the host. during t. gondii infection, the intestinal microbiota plays an important role in stimulating a protective immune response against the parasite. in this sense the use of probiotics is worthy of note since they are live microorganisms that have beneficial effec ...201627074320
bifidobacteria inhibit the growth of porphyromonas gingivalis but not of streptococcus mutans in an in vitro biofilm model.there is growing interest in the use of probiotic bifidobacteria for enhancement of the therapy, and in the prevention, of oral microbial diseases. however, the results of clinical studies assessing the effects of bifidobacteria on the oral microbiota are controversial, and the mechanisms of actions of probiotics in the oral cavity remain largely unknown. in addition, very little is known about the role of commensal bifidobacteria in oral health. our aim was to study the integration of the probi ...201627061393
probiotic strain bifidobacterium animalis subsp. lactis cect 8145 reduces fat content and modulates lipid metabolism and antioxidant response in caenorhabditis elegans.recently, microbial changes in the human gut have been proposed as a possible cause of obesity. therefore, modulation of microbiota through probiotic supplements is of great interest to support obesity therapeutics. the present study examines the functional effect and metabolic targets of a bacterial strain, bifidobacterium animalis subsp. lactis cect 8145, selected from a screening in caenorhabditis elegans. this strain significantly reduced total lipids (40.5% ± 2.4) and triglycerides (27.6% ± ...201627054371
phenolic acid degradation potential and growth behavior of lactic acid bacteria in sunflower substrates.sunflower flour provides a high content of protein with a well-balanced amino acid composition and is therefore regarded as an attractive source for protein. the use for human nutrition is hindered by phenolic compounds, mainly chlorogenic acid, which can lead under specific circumstances to undesirable discolorations. in this study, growth behavior and degradation ability of chlorogenic acid of four lactic acid bacteria were explored. data suggested that significant higher fermentation performa ...201627052717
probiotics and respiratory and gastrointestinal tract infections in finnish military conscripts - a randomised placebo-controlled double-blinded study.military conscripts are susceptible to respiratory and gastrointestinal tract infections. in previous studies probiotics have shown potency to reduce upper respiratory and gastrointestinal infections. the aim was to study whether probiotic intervention has an impact on seasonal occurrence of upper respiratory and gastrointestinal infections in two different conscript groups. in a randomised, double-blinded, placebo controlled study (https://clinicaltrials.gov nct01651195), a total of 983 healthy ...201627048835
effect of aqueous extract of the seaweed gracilaria domingensis on the physicochemical, microbiological, and textural features of fermented milks.the effects of the gracilaria domingensis seaweed aqueous extract in comparison with gelatin on the physicochemical, microbial, and textural characteristics of fermented milks processed with the mixed culture sab 440 a, composed of streptococcus thermophilus, lactobacillus acidophilus, and bifidobacterium animalis ssp. lactis, were investigated. the addition of g. domingensis aqueous extract did not affect ph, titratable acidity, and microbial viability of fermented milks when compared with the ...201626989840
comparative experimental investigation on the efficacy of mono- and multiprobiotic strains in non-alcoholic fatty liver disease prevention.to investigate the efficacy of different probiotic strains, their combinations and forms (alive or lyophilized) in nonalcoholic fatty liver disease (nafld) prevention.201626976285
supplementation of xylitol-containing chewing gum with probiotics: a double blind, randomised pilot study focusing on saliva flow and saliva properties.the aim of this study was to investigate the impact of daily chewing, for 12 weeks, of 2 different probiotic gums compared with placebo on saliva flow rate, saliva iga levels and saliva ph. the intervention study included 54 adult volunteers with hyposalivation in a double-blind, randomised and placebo-controlled design with three parallel groups. volunteers were randomly assigned to 3 different groups: subjects in group a (n = 19) were given placebo chewing gum, group b (n = 17) received bifido ...201626913493
probiotic yogurt culture bifidobacterium animalis subsp. lactis bb-12 and lactobacillus acidophilus la-5 modulate the cytokine secretion by peripheral blood mononuclear cells from patients with ulcerative colitis.there are some evidences for the immunomodulation disorders in the response to intestinal microbiota in inflammatory bowel disease. yogurt is a fermented milk product made with a starter culture consisting of different probiotics which could be colonized in intestine. however, the role of probiotics in the aetiopathogenesis of ulcerative colitis (uc) has not been clarified. to determine how the immune system responds to these bacteria this study was planned.201626909690
suppression subtractive hybridisation and real-time pcr for strain-specific quantification of the probiotic bifidobacterium animalis ban in broiler feed.to ensure quality management during the production processes of probiotics and for efficacy testing in vivo, accurate tools are needed for the identification and quantification of probiotic strains. in this study, a strain-specific qpcr assay based on suppression subtractive hybridisation (ssh) for identifying unique sequences, was developed to quantify the strain bifidobacterium animalis ban in broiler feed. seventy potential ban specific sequences were obtained after ssh of the ban genome, wit ...201626883620
the one-pot multienzyme (opme) synthesis of human blood group h antigens and a human milk oligosaccharide (hmos) with highly active thermosynechococcus elongates α1-2-fucosyltransferase.a novel α1-2-fucosyltransferase from thermosynechococcus elongatus bp-1 (te2ft) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. it was used in an efficient one-pot multienzyme (opme) fucosylation system for the high-yield synthesis of human blood group h antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (hmos) lacto-n-fucopentaose i (lnfp i) on preparative and gram scales. lnfp i was shown to be sele ...201626864394
glucolytic fingerprinting reveals metabolic groups within the genus bifidobacterium: an exploratory study.microorganisms of the genus bifidobacterium are inhabitants of diverse niches including the digestive tract of humans and animals. the species bifidobacterium adolescentis, bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve and bifidobacterium longum have qualified presumption of safety status granted by efsa and several strains are considered probiotic, and are being included in functional dairy fermented products. in the present work we carried out a preliminary explorati ...201626839073
consumption of bifidobacterium animalis subsp. lactis bb-12 impacts upper respiratory tract infection and the function of nk and t cells in healthy adults.probiotics can modulate immunity and reduce upper respiratory tract infections (urti) in humans; however few studies have examined both outcomes in the same trial. the goal of the current study was to investigate the effect of bifidobacterium animalis subsp. lactis bb-12, on natural killer (nk) and t-cell function in conjunction with self-reported cold/flu outcomes in healthy adults.201626821116
complete genome sequence of bifidobacterium animalis subsp. lactis klds 2.0603, a probiotic strain with digestive tract resistance and adhesion to the intestinal epithelial cells.bifidobacterium animalis subsp. lactis klds 2.0603 (abbreviated as klds 2.0603) is a probiotic strain isolated from the feces of an adult human. previous studies showed that klds 2.0603 has a high resistance to simulated digestive tract conditions and a high ability to adhere to intestinal epithelial cells (caco-2). these two characteristics are essential requirements for the selection of probiotic bacteria. to explore the stress resistance mechanism to the digestive tract environment and the ad ...201626795356
antimicrobial potential of commercial silver nanoparticles and the characterization of their physical properties toward probiotic bacteria isolated from fermented milk products.the application of nanotechnology in the agriculture and food sector is relatively recent compared to its usage in drug delivery or pharmaceuticals. therefore, this paper presents a study of the effect of silver nanoparticles on probiotic bacteria based on the example of lactobacillus acidophilus la-5, bifidobacterium animalis subsp. lactis bb-12 and streptococcus thermophilus st-y31 isolated from fermented milk products. probiotic bacteria are one of the most crucial groups of bacteria for the ...201626766747
clinical application of probiotics in type 2 diabetes mellitus: a randomized, double-blind, placebo-controlled study.type 2 diabetes has been associated with dysbiosis and one of the possible routes to restore a healthy gut microbiota is by the regular ingestion of probiotics. we aimed to investigate the effects of probiotics on glycemic control, lipid profile, inflammation, oxidative stress and short chain fatty acids in t2d.201726732026
using physical approaches for the attenuation of lactic acid bacteria in an organic rice beverage.a wild strain of lactobacillus plantarum, isolated from an italian sourdough, was inoculated in an organic rice drink; however, it caused a strong acidification. thus, it was preliminary processed through homogenization (single or multiple passes) or sonication (us) and then inoculated in the beverage. the samples were stored at 4 °c and analyzed to assess ph, production of lactic acid, viable count and sensory scores. a us-2-step process (power, 80%) could control acidification; viability and s ...201626678123
gut microbiota analysis reveals a marked shift to bifidobacteria by a starter infant formula containing a synbiotic of bovine milk-derived oligosaccharides and bifidobacterium animalis subsp. lactis cncm i-3446.non-digestible milk oligosaccharides were proposed as receptor decoys for pathogens and as nutrients for beneficial gut commensals like bifidobacteria. bovine milk contains oligosaccharides, some of which are structurally identical or similar to those found in human milk. in a controlled, randomized double-blinded clinical trial we tested the effect of feeding a formula supplemented with a mixture of bovine milk-derived oligosaccharides (bmos) generated from whey permeate, containing galacto-oli ...201626626365
consumption of bifidobacterium animalis subsp. lactis bb-12 in yogurt reduced expression of tlr-2 on peripheral blood-derived monocytes and pro-inflammatory cytokine secretion in young adults.probiotic bacteria modulate immune parameters and inflammatory outcomes. emerging evidence demonstrates that the matrix used to deliver probiotics may influence the efficacy of probiotic interventions in vivo. the aims of the current study were to evaluate (1) the effect of one species, bifidobacterium animalis subsp. lactis bb-12 at a dose of log10 ± 0.5 cfus/day on immune responses in a randomized, partially blinded, 4-period crossover, free-living study, and (2) whether the immune response to ...201726621631
health-promoting properties exhibited by lactobacillus helveticus strains.many strains belonging to lactobacilli exert a variety of beneficial health effects in humans and some of the bacteria are regarded as probiotic microorganisms. adherence and capabilities of colonization by lactobacillus strains of the intestinal tract is a prerequisite for probiotic strains to exhibit desired functional properties. the analysis conducted here aimed at screening strains of lactobacillus helveticus possessing a health-promoting potential. the molecular analysis performed, reveale ...201526601325
short- and long-term dynamics in the intestinal microbiota following ingestion of bifidobacterium animalis subsp. lactis gcl2505.bifidobacterium animalis subsp. lactis gcl2505 (b. lactis gcl2505) is able to survive passage through the intestines and proliferate. the daily dynamics of the intestinal bifidobacteria following ingestion of probiotics are not yet clear. moreover, the effects of long-term ingestion of probiotics on the intestinal microbiota have not been well studied. two experiments were performed in the present study. in experiment 1, 53 healthy female volunteers received b. lactis gcl2505; b. bifidum gcl2080 ...201526594607
proteolysis in goat "coalho" cheese supplemented with probiotic lactic acid bacteria.this study aimed to analyse the proteolytic effects of adding isolated and combined probiotic strains to goat "coalho" cheese. the cheeses were: qs - with culture start, composed by lactococcus lactis subsp. lactis and l. lactis subsp. cremoris (r704); qla - with lactobacillus acidophilus (la-5); qlp - with lactobacillus paracasei subsp. paracasei (l. casei 01); qb - with bifidobacterium animalis subsp. lactis (bb 12); and qc, co-culture with the three probiotic microorganisms. the cheeses were ...201626593502
probiotic supplementation influences faecal short chain fatty acids in infants at high risk for eczema.the composition of the gut microbiota plays a role in the development of allergies. based on the immunomodulating capacities of bacteria, various studies have investigated the potential role for probiotics in the prevention of childhood eczema. in a previous study we have shown that significantly less children developed eczema after probiotic supplementation (bifidobacterium bifidum w23, bifidobacterium animalis subsp. lactis w52 and lactococcus lactis w58, ecologic(®)panda) at three months of a ...201526565082
the gh5 1,4-β-mannanase from bifidobacterium animalis subsp. lactis bl-04 possesses a low-affinity mannan-binding module and highlights the diversity of mannanolytic enzymes.β-mannans are abundant and diverse plant structural and storage polysaccharides. certain human gut microbiota members including health-promoting bifidobacterium spp. catabolize dietary mannans. little insight is available on the enzymology of mannan deconstruction in the gut ecological niche. here, we report the biochemical properties of the first family 5 subfamily 8 glycoside hydrolase (gh5_8) mannanase from the probiotic bacterium bifidobacterium animalis subsp. lactis bl-04 (blman5_8).201526558435
discovery of novel small molecule modulators of clavibacter michiganensis subsp. michiganensis.clavibacter michiganensis subsp. michiganensis (cmm) is a gram-positive seed-transmitted bacterial phytopathogen responsible for substantial economic losses by adversely affecting tomato production worldwide. a high-throughput, cell-based screen was adapted to identify novel small molecule growth inhibitors to serve as leads for future bactericide development. a library of 4,182 compounds known to be bioactive against saccharomyces cerevisiae was selected for primary screening against cmm wild-t ...201526539169
intestinal microbiota in neonates requiring urgent surgery: assessing the role of probiotics using fecal dna sequencing.to assess the impact of urgent surgery on neonates and the value of an orally administered probiotic preparation of bifidobacterium animalis subsp. lactis lkm512 (lkm) using fecal dna sequencing to analyze intestinal microbiota.201626516074
the longitudinal effect of a multi-strain probiotic on the intestinal bacterial microbiota of neonatal foals.the microbiota plays a key role in health and disease. probiotics are a potential way to therapeutically modify the intestinal microbiota and prevent disease.201626509834
antigenotoxic activity of lactic acid bacteria, prebiotics, and products of their fermentation against selected mutagens.dietary components such as lactic acid bacteria (lab) and prebiotics can modulate the intestinal microbiota and are thought to be involved in the reduction of colorectal cancer risk. the presented study measured, using the comet assay, the antigenotoxic activity of both probiotic and non-probiotic lab, as well as some prebiotics and the end-products of their fermentation, against fecal water (fw). the production of short chain fatty acids by the bacteria was quantified using hplc. seven out of t ...201526404012
effect of the probiotic strain bifidobacterium animalis subsp. lactis, bb-12®, on defecation frequency in healthy subjects with low defecation frequency and abdominal discomfort: a randomised, double-blind, placebo-controlled, parallel-group trial.the aim of the present study was to investigate the effect of bifidobacterium animalis subsp. lactis, bb-12®, on two primary end points - defecation frequency and gastrointestinal (gi) well-being - in healthy adults with low defecation frequency and abdominal discomfort. a total of 1248 subjects were included in a randomised, double-blind, placebo-controlled trial. after a 2-week run-in period, subjects were randomised to 1 or 10 billion colony-forming units/d of the probiotic strain bb-12® or a ...201526382580
prebiotic preferences of human lactobacilli strains in co-culture with bifidobacteria and antimicrobial activity against clostridium difficile.to evaluate robustness, prebiotic utilization of lactobacillus paracasei f8 and lactobacillus plantarum f44 in mono- and co-cultures with bifidobacterium breve 46 and bifidobacterium animalis sub sp. lactis 8 : 8 and antimicrobial activity of co-culture against clostridium difficile.201526381324
bifidobacterium animalis subsp. lactis bb-12 in reducing the risk of infections in early childhood.specific probiotic bacteria have proven to be effective in the prevention and treatment of infectious diseases in early life in at-risk populations. the impact of administration of bifidobacterium animalis subsp. lactis bb-12 (bb-12) on the risk of acute infectious diseases was studied in healthy children.201626372517
probiotic b420 and prebiotic polydextrose improve efficacy of antidiabetic drugs in mice.gut microbiota is now known to control glucose metabolism. previous studies have shown that probiotics and prebiotics may improve glucose metabolism, but their effects have not been studied in combination with drug therapy. the aim of this study was to investigate whether probiotics and prebiotics combined with drug therapy affect diabetic outcomes.201526366205
a single mutation in the gene responsible for the mucoid phenotype of bifidobacterium animalis subsp. lactis confers surface and functional characteristics.exopolysaccharides (eps) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. we have sequenced the genomes of two isogenic strains of bifidobacterium animalis subsp. lactis that differ in their eps-producing phenotype. the original strain displays a nonmucoid appearance, and the mutant derived thereof has acquired a mucoid phenotype. the sequence anal ...201526362981
evaluation of viability bifidobacterium animalis subsp. lactis lkm512 in dogs.in dogs, gastric acid is not neutralised even when a meal is present in the stomach. moreover, dogs take longer to digest their meals than humans do. accordingly, the most important characteristic of any probiotics considered for use in dogs is high acid tolerance. the probiotic strain bifidobacterium animalis subsp. lactis lkm512 (hereafter referred to as lkm512) not only exhibits potent acid tolerance, but also has the ability to adhere to intestinal mucin. the aim of the present study was to ...201526322543
[new approaches for the isolation of bifidobacterial strains, their molecular characterization, and assessment of their probiotic potential].six stably growing strains of bifidobacteria possessing probiotic properties were isolated from the feces of newborn children and animals. according to the results of molecular analysis, one strain was classified as bifidobacterium bifidum, while five belonged to bifidobacterium animalis. initial identification of the strains was carried out using the primer pairs for the 16s rrna gene (g-bifid-f/r, bif164/662) and for the xfp gene specific for bifidobacteria. subsequent sequencing of complete g ...201526263695
microencapsulation of new probiotic formulations for gastrointestinal delivery: in vitro study to assess viability and biological properties.the paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, lactobacillus plantarum pbs067, lactobacillus rhamnosus pbs070, and bifidobacterium animalis subsp. lactis pbs075 taken individually and as a mixture of them. the effects of microencapsulation on the viability of the strains in conditions simulating the gastrointestinal tract and under industrial processes conditions were studied. in addi ...201526239070
comparative genomics revealed genetic diversity and species/strain-level differences in carbohydrate metabolism of three probiotic bifidobacterial species.strains of bifidobacterium longum, bifidobacterium breve, and bifidobacterium animalis are widely used as probiotics in the food industry. although numerous studies have revealed the properties and functionality of these strains, it is uncertain whether these characteristics are species common or strain specific. to address this issue, we performed a comparative genomic analysis of 49 strains belonging to these three bifidobacterial species to describe their genetic diversity and to evaluate spe ...201526236711
in vitro activity of tea tree oil vaginal suppositories against candida spp. and probiotic vaginal microbiota.the aim of this work is to evaluate the in vitro microbicidal activity of vaginal suppositories (vs) containing tea tree oil (tto-vs) towards candida spp. and vaginal probiotics. a total of 20 candida spp. strains, taken from patients with vaginitis and from an established type collection, including reference strains, were analysed by using the clsi microdilution method. to study the action of vs towards the beneficial vaginal microbiota, the sensitivity of bifidobacterium animalis subsp. lactis ...201526235937
perinatal probiotic supplementation in the prevention of allergy related disease: 6 year follow up of a randomised controlled trial.perinatal probiotics supplementation has been shown to be effective in the primary prevention of atopic dermatitis (ad) in early childhood, although the long term effects of probiotics on ad and other allergic diseases is less certain. we have previously reported a significant reduction in the cumulative incidence of ad at 2 years after maternal probiotic supplementation. in this study we present the effects of perinatal probiotics given to women from a general population on allergy related dise ...201526232126
fermentation performance of lactic acid bacteria in different lupin substrates-influence and degradation ability of antinutritives and secondary plant metabolites.the main objectives were to determine the influence of secondary plant metabolites and antinutritives in lupin seeds on the fermentation performance of lactic acid bacteria and to study their ability to degrade these substances. the suitability of lupin raw materials as fermentation substrates was examined.201526189714
rna-seq reveals transcriptomic interactions of bacillus subtilis natto and bifidobacterium animalis subsp. lactis in whole soybean solid-state co-fermentation.bifidobacteria are anaerobes and are difficult to culture in conventional fermentation system. it was observed that bacillus subtilis natto enhanced growth of bifidobacterium animalis subsp. lactis v9 by about 3-fold in a whole soybean solid-state co-fermentation, in a non-anaerobic condition. for the purpose of understanding the metabolic interactions between bif. animalis subsp. lactis v9 and ba. subtilis natto, the transcriptome of bif. animalis subsp. lactis v9 and ba. subtilis natto was ana ...201526187824
solid-state fermentation of whole oats to yield a synbiotic food rich in lactic acid bacteria and prebiotics.this study developed a synbiotic food through the fermentation of whole oat flour with lactobacillus plantarum tk9 and bifidobacterium animalis subsp. lactis v9. the physicochemical properties, changes in ingredients and peptide molecular weight distributions were determined during the whole oat fermentation. the highest viable counts of the fermented oats were 2.85 × 10(9) cfu g(-1) (l. plantarum tk9) and 3.17 × 10(8) cfu g(-1) (bif. animalis subsp. lactis v9), with the titratable acidity incre ...201526130143
the role of beneficial bacteria wall elasticity in regulating innate immune response.probiotics have great potential to contribute to development of healthy dietary regimes, preventive care, and an integrated approach to immunity-related disease management. the bacterial wall is a dynamic entity, depending on many components and playing an essential role in modulating immune response. the impact of cell wall elasticity on the beneficial effects of probiotic strains has not been sufficiently studied. the aim was to investigate the effect of lactic acid bacteria (lab) and bifidoba ...201526110044
effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by bifidobacterium.the aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (epss) produced by bifidobacterium. cigarettes of shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (csc). the standard strain of bifidobacterium animalis was cultured in mrs media under anaerobic addition of csc. the results showed that csc significantly decreased the growth of b. animalis as well as epss and acetic a ...201526062117
immunomodulatory effects of probiotic supplementation in schizophrenia patients: a randomized, placebo-controlled trial.although peripheral immune system abnormalities have been linked to schizophrenia pathophysiology, standard antipsychotic drugs show limited immunological effects. thus, more effective treatment approaches are required. probiotics are microorganisms that modulate the immune response of the host and, therefore, may be beneficial to schizophrenia patients. the aim of this study was to examine the possible immunomodulatory effects of probiotic supplementation in chronic schizophrenia patients. the ...201526052224
[effects of mono-, poly- and composite probiotics on the ulceration caused by restraint stress].it was studied the effect of probiotic strains of bifidobacterium animalis vkl, bifidobacterium animalis vkb and lactobacillus casei imvb-7280, and their mixtures on erosive and ulcerative lesions of the gastric mucosa (gm) of rats. gm was induced by water-immersion restraint stress. it was found that investigated probiotics did not have gastroprotective properties under a single and seven-day prophylactic administration. however, multiprobiotics (polyprobiotic bifidobacterium animalis vkl and b ...201526040033
bifidobacterium animalis subsp. lactis in prevention of common infections in healthy children attending day care centers - randomized, double blind, placebo-controlled study.the aim of our study was to investigate the role of bifidobacterium animalis subsp. lactis (bb-12(®)) in the prevention of common (gastrointestinal and respiratory) infections in healthy children who attend day care centers.201626031908
microencapsulation of probiotic bacteria using thermo-sensitive sol-gel polymers for powdered infant formula.in this study the application of thermo-sensitive sol-gel polymers in microencapsulation formulation of probiotic bacteria, bifidobacterium animalis spp lactis, for powdered infant formula (pif), which is reconstituted at 70 °c, has been assessed. a double-layered microcapsule containing hydroxypropyl methyl cellulose (hpmc) as an inner layer and an outer layer, as the smart coating layer, based on a combination of hydroxypropyl cellulose (hpc) and poloxamer was designed. generally, this specifi ...201526004368
production of bacteriocin-like inhibitory substance by bifidobacterium lactis in skim milk supplemented with additives.bacteriocins are natural compounds used as food biopreservatives instead of chemical preservatives. bifidobacterium animalis subsp. lactis (bifid. lactis) was shown to produce a bacteriocin-like inhibitory substance (blis) able to inhibit the growth of listeria monocytogenes selected as an indicator microorganism. to enhance this production by the strain bifid. lactis bl 04, skim milk (sm) was used as a fermentation medium either in the presence or in the absence of yeast extract, tween 80 or in ...201525850555
effect of sublethal preculturing on the survival of probiotics and metabolite formation in set-yoghurt.the objective of this study was to investigate the effect of preculturing of lactobacillus rhamnosus gg and bifidobacterium animalis subsp. lactis bb12 under sublethal stress conditions on their survival and metabolite formation in set-yoghurt. prior to co-cultivation with yoghurt starters in milk, the two probiotic strains were precultured under sublethal stress conditions (combinations of elevated nacl and low ph) in a batch fermentor. the activity of sublethally precultured probiotics was eva ...201525846920
randomized, double-blind, placebo-controlled study of synbiotic yogurt effect on the health of children.to assess the effects of daily consumption of a synbiotic yogurt drink on the health, growth, and quality of life of healthy children 12-48 months of age in out-of-home child care.201525841539
proteolytic and ace-inhibitory activities of probiotic yogurt containing non-viable bacteria as affected by different levels of fat, inulin and starter culture.in this study, the effects of fat (0.5 %, 3.2 % and 5.0 %), inulin (0.0 and 1.0 %) and starter culture (0.0 %, 0.5 %, 1.0 % and 1.5 %) on the angiotensin converting enzyme (ace)-inhibitory activity of probiotic yogurt containing non-viable bacteria were assessed. proteolytic activities of bacteria were also investigated. yogurts were prepared either using a sole yogurt commercial culture including streptococcus thermophilus and lactobacillus delbrueckii subs. bulgaricus or bifidobacterium animal ...201525829629
does maternal perinatal probiotic supplementation alter the intestinal microbiota of mother and child?maternal probiotic supplementation has been shown to prevent the development of atopic dermatitis in the offspring. we aimed to investigate whether probiotics in pregnant and breast-feeding mothers altered the colonization pattern and the diversity of the mothers' and children's intestinal microbiota.201525782657
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