Publications

molecular regulation of temperature-dependent floral induction in tulipa gesneriana.	the vegetative-to-reproductive phase change in tulip (tulipa gesneriana) is promoted by increasing temperatures during spring. the warm winters of recent years interfere with this process and are calling for new adapted cultivars. a better understanding of the underlying molecular mechanisms would be of help, but unlike the model plant arabidopsis (arabidopsis thaliana), very little is known about floral induction in tulip. to shed light on the gene regulatory network controlling flowering in tu ...	2017	28104719
dietary effects on body weight of predatory mites (acari, phytoseiidae).	pollen is offered as alternative or supplementary food for predacious mites; however, it may vary in its nutritional value. body weight appears a representative parameter to describe food quality. thus, we assessed the body weight for adults of the generalist mites amblyseius swirskii, amblydromalus limonicus, and neoseiulus cucumeris reared on 22, 12, and 6 pollen species, respectively. in addition, a. swirskii and a. limonicus was reared on codling moth eggs. in all mite species, female body w ...	2015	26014648
genetic mapping of resistance to fusarium oxysporum f. sp. tulipae in tulip.	fusarium oxysporum is a major problem in the production of tulip bulbs. breeding for resistant cultivars through a conventional approach is a slow process due to the long life cycle of tulip. until now, marker-assisted selection (mas) has been hampered by the large genome size and the absence of a genetic map. this study is aimed at construction of the first genetic map for tulip and at the identification of loci associated with resistance to f. oxysporum. a cross-pollinated population of 125 in ...	2017	25983613
hormonal regulation of gummosis and composition of gums from bulbs of hyacinth (hyacinthus orientalis).	hyacinth (hyacinthus orientalis) bulbs infected by fusarium oxysporum showed the symptoms of gummosis. the purpose of this study was to clarify the hormonal regulation of gummosis and composition of gums from hyacinth bulbs. the application of ethephon (2-chloroethylphosphonic acid), an ethylene-releasing compound, at 2% (w/w, in lanolin) induced gummosis in hyacinth bulbs. methyl jasmonate (ja-me) at 1.5% (w/w, in lanolin) induced gummosis as well. simultaneous application of ja-me and ethephon ...	2015	25462960
carbon dioxide fixation by epidermal and mesophyll tissues of tulipa and commelina.	rates of (14)co2 fixation by epidermal tissue of tulipa gesneriana (tulip) and commelina diffusa are only slightly higher in the light than in the dark while in mesophyl tissues rates are much greater in the light. the first products of (14)co2 fixation by epidermal tissue of tulipa gesneriana and c. diffusa in the light and dark are malate and aspartate. in addition to these dominating dicarboxylic acids, 3-phosphoglyceric acid and sugar phosphates appear in the light, while in the dark only th ...	1974	24458325
the identification of a vacuolar iron transporter involved in the blue coloration of cornflower petals.	the blue petal color of the cornflower (centaurea cyanus) is caused by protocyanin, a kind of metalloanthocyanin, which is a self-assembled supramolecular metal complex pigment. protocyanin is composed of six molecules of anthocyanin, six molecules of flavone, one ferric ion, and one magnesium ion. the ferric ion is essential for blue color development. here, we identify the vacuolar iron transporter gene (ccvit) from the blue petals of c. cyanus and its function is identified and characterized. ...	2013	23838627
a vacuolar iron transporter in tulip, tgvit1, is responsible for blue coloration in petal cells through iron accumulation.	blue color in flowers is due mainly to anthocyanins, and a considerable part of blue coloration can be attributed to metal-complexed anthocyanins. however, the mechanism of metal ion transport into vacuoles and subsequent flower color development has yet to be fully explored. previously, we studied the mechanism of blue color development specifically at the bottom of the inner perianth in purple tulip petals of tulipa gesneriana cv. murasakizuisho. we found that differences in iron content were ...	2009	19366427
characterization of four plasma membrane aquaporins in tulip petals: a putative homolog is regulated by phosphorylation.	we suggested previously that temperature-dependent tulip (tulipa gesneriana) petal movement that is concomitant with water transport is regulated by reversible phosphorylation of an unidentified plasma membrane intrinsic protein (pip). in this study, four full-length cdnas of pips from tulip petals were identified and cloned. two pips, namely tgpip1;1 and tgpip1;2, are members of the pip1 subfamily, and the remaining two pips, namely tgpip2;1 and tgpip2;2, belong to the pip2 subfamily of aquapor ...	2008	18567892
on the resistance to transpiration of the sites of evaporation within the leaf.	the rates of transpiration from the upper and lower surfaces of leaves of gossypium hirsutum, xanthium strumarium, and zea mays were compared with the rates at which helium diffused across those leaves. there was no evidence for effects of co(2) concentration or rate of evaporation on the resistance to water loss from the evaporating surface ("resistance of the mesophyll wall to transpiration") and no evidence for any significant wall resistance in turgid tissues. the possible existence of a wal ...	1978	16660404
roquefortine/oxaline biosynthesis pathway metabolites in penicillium ser. corymbifera: in planta production and implications for competitive fitness.	three strains of each of the seven taxa comprising the penicillium series corymbifera were surveyed by direct injection mass spectrometry (ms) and liquid chromatography-ms for the production of terrestric acid and roquefortine/oxaline biosynthesis pathway metabolites when cultured upon macerated tissue agars prepared from allium cepa, zingiber officinale, and tulipa gesneriana, and on the defined medium czapek yeast autolysate agar (cya). a novel solid-phase extraction methodology was applied fo ...	2005	16195849
[animal nutrition for veterinarians--actual cases: tulip bulbs with leaves (tulipa gesneriana)--an unusual and high risk plant for ruminant feeding].	14 cattle (mainly younger ones) of a total of 50 extensively kept galloways died within 6 weeks in late winter 2001/02. according to the owner's report, grass growth had been rather poor; therefore, the herd was fed additionally hay as well as large amounts of tulip onions. in the microbiological examination a highly reduced hygienic quality of the roughage could be detected. in the rumen contents of two dissected young cattle parts of tulip onions were found. according to pertinent literature, ...	2003	12910870
accumulation of plant small heat-stress proteins in storage organs.	plant small heat-stress proteins (shsps) have been shown to be expressed not only after exposure to elevated temperatures, but also at particular developmental stages such as embryogenesis, microsporogenesis, and fruit maturation. this paper presents new data on the occurrence of shsps in vegetative tissues, their tissue-specific distribution, and cellular localization. we have found shsps in 1-year-old twigs of acer platanoides l. and sambucus nigra l. and in the liana aristolochia macrophylla ...	2002	12029471
a novel histone variant localized in nucleoli of higher plant cells.	immunofluorescence staining with antisera raised against p35, a basic nuclear protein that accumulates in the pollen nuclei of lilium longiflorum, specifically stained the nucleoli in interphase nuclei of somatic tissues, including root and leaf, and in pachytene nuclei during meiotic division, whereas antisera raised against histone h1 uniformly stained the entire chromatin domain with the exception of the nucleoli in these nuclei. further, p35-specific antisera stained the nucleoli in root and ...	1999	10398848
flow injection analysis of binding reaction between fluorescent lectin and cells.	a fluorometric binding assay for lectin and yeast cells using the avidin-biotin system was previously reported (y. oda, m. kinoshita, and k. kakehi, anal. biochem. 254, 41-48, 1997). however, the true amount of bound lectin could not be determined by this method due to difficulty in determination of the number of bound biotin molecules. in the present study, we have developed a method for assaying the binding reaction between fluorescent lectin and cells using a flow injection technique, which a ...	1999	10221994
microvascular protective activity of flavonoid glucuronides fraction from tulipa gesneriana.	a mixture of flavonoid glucuronides, consisting of 7-o-glucuronides of kaempferol and quercetin 3-o-rutinosides, 3-o-gentiobiosides and 3-o-glucosides, was isolated from the perianths of tulipa gesneriana l. var. 'paradae'. it showed protective activity against the increased (both chloroform and histamine) skin vascular permeability in rabbits. the protective effect, measured as the reduction in leakage of evans blue, was 59.8% after peritoneal treatment at a dose of 25 mg/kg, while that of trox ...	1999	10190195
cloning of sucrose:sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose.	sucrose (suc):suc 1-fructosyltransferase (1-sst) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from suc. we have cloned 1-sst from onion (allium cepa) by screening a cdna library using acid invertase from tulip (tulipa gesneriana) as a probe. expression assays in tobacco (nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose from suc. in addition, an onion acid invertase clone was isolated from the same cdna library. protein extr ...	1998	9701606
successful expression in pollen of various plant species of in vitro synthesized mrna introduced by particle bombardment.	gold particles coated with beta-glucuronidase (gus) mrna with a 5' cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (lilium longiflorum), freesia (freesia refracta) and tulip (tulipa gesneriana). a fluorometric assay for the gus activity indicated that in vitro synthesized gus mrna introduced into these pollen cells by particle bombardment was successfully expressed. gus activity in extracts of the bombarded lily pol ...	1995	7599318
isolation of immunochemically distinct form of cytochrome p-450 from microsomes of tulip bulbs.	a highly purified cytochrome p-450 was obtained from the microsomes of tulip bulbs (tulipa gesneriana l.). the molecular weight (mr = 52,500) and amino acid composition of this plant cytochrome p-450 are similar to those reported for rat livers. on the contrary, ouchterlony double diffusion analyses indicated that cytochrome p-450 isolated from tulip bulbs shares no common antigenic determinants with those of 9 other plants, in spite of the presence of comparable contents of cytochrome p-450 and ...	1983	6412714
[on antimycotic substances from tulipa gesneriana].		1966	6011531
[action of antibiotic substances from the garden tulip tulipa gesneriana on bacteria].		1970	4990222
isolation and characterization of a lectin from tulip bulbs, tulipa gesneriana.	a lectin, which agglutinated specifically the yeast cells of the saccharomyces genus, was isolated from tulip bulbs (tulipa gesneriana) using affinity chromatography on mannan-sepharose 4b. its relative molecular mass was determined by gel filtration to be approximately 67,000. on polyacrylamide gel electrophoresis in sodium dodecyl sulfate, a relative molecular mass of 17,000 was obtained, suggesting that the lectin is a tetramer. binding studies performed with iodinated lectin indicated that s ...	1986	3758061
a new agglutinin from the tulipa gesneriana bulbs.	two agglutinins with different agglutinating activity exist in tulipa gesneriana bulbs. one is the t. gesneriana lectin which agglutinates yeasts as reported previously [oda, y. and minami, k. (1986) eur. j. biochem. 159, 239-245]. the other agglutinin is a new one which agglutinates animal erythrocytes and was purified from the tulip bulbs using affinity chromatography on thyroglobulin-sepharose 4b. the agglutinin agglutinated mouse and rat erythrocytes at a minimum concentration of 2 microgram ...	1987	3595592
tulipa gesneriana bulb extracts activate promutagenic 7,12-dimethylbenz[a]anthracene in the salmonella/ames assay.	crude extracts from tulipa gesneriana bulbs have been tested for their ability to activate 7,12-dimethylbenz[a]anthracene (dmba) in the salmonella mutagenicity assay. bacteria of strain ta98 were incubated for 30 min at 37 degrees c with the mixture of the promutagen and bulb extracts prior to plating. the frequency of his+ revertants increased in relation to both the promutagenic dose and the amount of bulb extract in the mixture, and under optimal conditions, was more than 50 times higher than ...	1986	3088443
mitogenic activity of tulipa gesneriana lectins on mouse and human lymphocytes.	tulipa gesneriana lectin-erythrocyte (tgl-e) which agglutinates mouse erythrocytes showed a potent mitogenic activity on mouse spleen cells and human peripheral blood lymphocytes, however, tgl-e had only slight mitogenic activity on mouse thymus cells. its subunit alpha with a molecular weight (mw) of about 26,000 showed a potent mitogenic activity as did that of native lectin, but subunit beta with a mw of about 14,000 showed no activity, indicating that the mitogenic activity of tgl-e originat ...	1991	1814629