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rna-dependent rna polymerase (nib) of the potyviruses is an avirulence factor for the broad-spectrum resistance gene pvr4 in capsicum annuum cv. cm334.potyviruses are one of the most destructive viral pathogens of solanaceae plants. in capsicum annuum landrace cm334, a broad-spectrum gene, pvr4 is known to be involved in resistance against multiple potyviruses, including pepper mottle virus (pepmov), pepper severe mosaic virus (pepsmv), and potato virus y (pvy). however, a potyvirus avirulence factor against pvr4 has not been identified. to identify the avirulence factor corresponding to pvr4 in potyviruses, we performed agrobacterium-mediated ...201525760376
[sequence variation and protein structure of pipo gene in potato virus y].the objectives of this study were to understand the sequence variation and the putative protein structure of pipo gene in the potato virus y (pvy) collected from solanum tuberosum. the pipo gene in pvy was cloned using a pair of degenerate primers designed from its conserved region and its sequences were used to re-construct phylogenetic tree in potyvirus genera by a bayesian inference method. an expected fragment of 235 bp was amplified in all 20 samples by rt-pcr and the pipo genes in the 20 s ...201324400487
the complete genome sequence of pepper severe mosaic virus and comparison with other potyviruses.the complete nucleotide sequence of pepper severe mosaic virus (pepsmv) was determined. the viral genome consisted of 9890 nucleotides, excluding a poly (a) tract at the 3' end of the genome. the pepsmv rna genome encoded a single polyprotein of 3085 amino acid residues, resulting in ten functionally distinct potyviral proteins. the lengths of the 5' nontranslated region (ntr) and the 3' ntr were 164 and 468 nucleotides, respectively. the genome organization of the virus was typical for members ...200616699829
molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in peru.peru is a centre of origin and domestication of the potato, pepper and tomato (family solanaceae). many potyviruses (genus potyvirus) that infect these crops were described 20-30 years ago. however, definitive classification of these viruses as distinct species remains unresolved for several reasons, including their close serological relationships, similar symptomatology in test plants and lack of genomic sequence data. using samples collected from peru, we have determined the complete genomic s ...200312917478
pepper yellow mosaic virus, a new potyvirus in sweetpepper, capsicum annuum.a potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in central and southeast brazil. the sequence analysis of the 3' terminal region of the viral rna revealed a coat protein of 278 amino acids, followed by 275 nucleotides in the 3'-untranslated region preceding a polyadenylated tail. the virus shared 77.4% coat protein amino acid identity with pepper severe mosaic virus, the closest potyvirus species. the 3'-untranslated region was highly divergent from other potyviruse ...200212038694
cdna sequence of the pepper severe mosaic virus coat protein gene. 19938022927
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