Publications

a novel bunyavirus causing fever and thrombocytopenia: more questions than answers.		2011	22220348
Antiviral escape strategies developed by bunyaviruses pathogenic for humans.	New or re-emerging pathogens for humans have emerged outside of their usual endemic range during the last decade originating severe public health concern and economical losses. Climate changes have played a significant role in the emergence or re-emergence of arboviruses. Among these pathogens, several viruses belong to the Bunyaviridae family. This family is composed of RNA viruses grouped into five genera Orthobunyavirus, Hantavirus, Nairovirus, Phlebovirus and Tospovirus characterized by thei ...	2012	22202109
[pyrexia and thrombocytopenia syndrome: a novel bunyavirus infectious disease].		2011	22176956
hemorrhagic fever caused by a novel bunyavirus in china: pathogenesis and correlates of fatal outcome.	background. hemorrhagic fever-like illness caused by a novel bunyavirus, huaiyangshan virus (hysv, also known as severe fever with thrombocytopenia virus [sftsv] and fever, thrombocytopenia and leukopenia syndrome [ftls]), has recently been described in china.methods. patients with laboratory-confirmed hysv infection who were admitted to union hospital or zhongnan hospital between april 2010 and october 2010 were included in this study. clinical and routine laboratory data were collected and blo ...	2011	22144540
Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus.	Since 2007, many cases of fever, thrombocytopenia and leukopenia syndrome (FTLS) have emerged in Henan Province, China. Patient reports of tick bites suggested that infection could contribute to FTLS. Many tick-transmitted microbial pathogens were tested for by PCR/RT-PCR and/or indirect immunofluorescence assay (IFA). However, only 8% (24/285) of samples collected from 2007 to 2010 tested positive for human granulocytic anaplasmosis (HGA), suggesting that other pathogens could be involved. Here ...	2011	22114553
person-to-person transmission of severe fever with thrombocytopenia syndrome bunyavirus through blood contact.	severe fever with thrombocytopenia syndrome bunyavirus is a newly discovered bunyavirus with high pathogenicity to human. the transmission model has been largely uncharacterized. investigation on a cluster of severe fever with thrombocytopenia syndrome cases provided evidence of person-to-person transmission through blood contact to the index patient with high serum virus load.	2012	22095565
Histone deacetylase 8 is required for centrosome cohesion and influenza A virus entry.	Influenza A virus (IAV) enters host cells by endocytosis followed by acid-activated penetration from late endosomes (LEs). Using siRNA silencing, we found that histone deacetylase 8 (HDAC8), a cytoplasmic enzyme, efficiently promoted productive entry of IAV into tissue culture cells, whereas HDAC1 suppressed it. HDAC8 enhanced endocytosis, acidification, and penetration of the incoming virus. In contrast, HDAC1 inhibited acidification and penetration. The effects were connected with dramatic alt ...	2011	22046129
a family cluster of infections by a newly recognized bunyavirus in eastern china, 2007: further evidence of person-to-person transmission.	seven persons in one family living in eastern china developed fever and thrombocytopenia during may 2007, but the initial investigation failed to identify an infectious etiology. in december 2009, a novel bunyavirus (designated severe fever with thrombocytopenia syndrome bunyavirus [sftsv]) was identified as the cause of illness in patients with similar clinical manifestations in china. we reexamined this family cluster for sftsv infection.	2011	22028437
early diagnosis of novel sfts bunyavirus infection by quantitative real-time rt-pcr assay.	severe fever with thrombocytopenia syndrome (sfts) is an emerging infectious disease recently identified to be caused by a novel bunyavirus (sftsv). the clinical diagnosis is urgently needed to differentiate the disease from other infections.	2012	22024488
rift valley fever virus inhibits a pro-inflammatory response in experimentally infected human monocyte derived macrophages and a pro-inflammatory cytokine response may be associated with patient survival during natural infection.	rift valley fever virus (rvfv) causes significant morbidity and mortality in humans and livestock throughout africa and the middle east. the clinical disease ranges from mild febrile illness, to hepatitis, retinitis, encephalitis and fatal hemorrhagic fever. rvfv nss protein has previously been shown to interfere in vitro with the interferon response, and rvfv lacking the nss protein is attenuated in several animal models. monocytes and macrophages are key players in the innate immune response v ...	2012	22018491
A Recombinant Chimeric La Crosse Virus Expressing the Surface Glycoproteins of Jamestown Canyon Virus Is Immunogenic and Protective against Challenge with either Parental Virus in Mice or Monkeys.	La Crosse virus (LACV) and Jamestown Canyon virus (JCV), family Bunyaviridae, are mosquito-borne viruses that are endemic in North America and recognized as etiologic agents of encephalitis in humans. Both viruses belong to the California encephalitis virus serogroup, which causes 70 to 100 cases of encephalitis a year. As a first step in creating live attenuated viral vaccine candidates for this serogroup, we have generated a recombinant LACV expressing the attachment/fusion glycoproteins of JC ...	2012	22013033
bunyaviruses and the type i interferon system.	the family bunyaviridae contains more than 350 viruses that are distributed throughout the world. most members of the family are transmitted by arthopods, and several cause disease in man, domesticated animals and crop plants. despite being recognized as an emerging threat, details of the virulence mechanisms employed by bunyaviruses are scant. in this article we summarise the information currently available on how these viruses are able to establish infection when confronted with a powerful ant ...	2009	21994579
creation of a nonspreading rift valley fever virus.	rift valley fever virus (rvfv) is a mosquito-borne zoonotic bunyavirus of the genus phlebovirus and a serious human and veterinary pathogen. rvfv contains a three-segmented rna genome, which is comprised of the large (l), medium (m), and small (s) segments. the proteins that are essential for genome replication are encoded by the l and s segments, whereas the structural glycoproteins are encoded by the m segment. we have produced bhk replicon cell lines (bhk-rep) that maintain replicating l and ...	2011	21957302
Person-to-Person Transmission of Severe Fever with Thrombocytopenia Syndrome Virus.	Abstract Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a newly discovered bunyavirus, SFTS virus (SFTSV), and causes high fatality (12% on average and as high as 30%). The objective of this study was to determine whether SFTSV could be transmitted from person to person. We analyzed sera of 13 patients from two clusters of unknown infectious diseases that occurred between September and November of 2006 in Anhui Province of China for SFTSV antibod ...	2011	21955213
iquitos virus: a novel reassortant orthobunyavirus associated with human illness in peru.	oropouche (oro) virus, a member of the simbu serogroup, is one of the few human pathogens in the orthobunyavirus genus in the family bunyaviridae. genetic analyses of oro-like strains from iquitos, peru, identified a novel reassortant containing the s and l segments of oro virus and the m segment of a novel simbu serogroup virus. this new pathogen, which we named iquitos (iqt) virus, was first isolated during 1999 from a febrile patient in iquitos, an amazonian city in peru. subsequently, the vi ...	2011	21949892
Interference in plant defense and development by non-structural protein NSs of Groundnut bud necrosis virus.	Groundnut bud necrosis virus (GBNV) infects a large number of leguminous and solanaceous plants. To elucidate the biological function of the non-structural protein encoded by the S RNA of GBNV (NSs), we studied its role in RNA silencing suppression and in viral pathogenesis. Our results demonstrated that GBNV NSs functions as a suppressor of RNA silencing using the agroinfiltration patch assay. An in silico analysis suggested the presence of pro-apoptotic protein Reaper-like sequences in the GBN ...	2011	21924303
A novel bunyavirus in China.		2011	21879913
a novel bunyavirus in china.		2011	21879912
a novel bunyavirus in china.		2011	21879911
Recent advances in the molecular and cellular biology of bunyaviruses.	The family Bunyaviridae of segmented, negative-stranded RNA viruses includes over 350 members that infect a bewildering variety of animals and plants. Many of these bunyaviruses are the causative agents of serious disease in their respective hosts, and are classified as emerging viruses because of their increased incidence in new populations and geographical locations throughout the world. Emerging bunyaviruses, such as Crimean-Congo hemorrhagic fever virus, tomato spotted wilt virus and Rift Va ...	2011	21865443
[fever with thrombocytopenia associated with a novel bunyavirus in china].		2011	21863623
[insect-borne diseases and insect bites in finland].	clinically significant endemic, arthropod-borne infectious diseases in finland include lyme disease, tick-borne encephalitis, tularemia and karelian fever. the diagnosis of early borreliosis or tularemia is clinical and the treatment is initiated already before the results of eventual blood tests. the diagnosis of late stage borreliosis, tick-borne encephalitis or karelian fever is confirmed mainly with serologic tests. the clinical significance of endemic anaplasmosis, rickettsia helvetica, ink ...	2011	21834346
broad-spectrum antiviral therapeutics.	currently there are relatively few antiviral therapeutics, and most which do exist are highly pathogen-specific or have other disadvantages. we have developed a new broad-spectrum antiviral approach, dubbed double-stranded rna (dsrna) activated caspase oligomerizer (draco) that selectively induces apoptosis in cells containing viral dsrna, rapidly killing infected cells without harming uninfected cells. we have created dracos and shown that they are nontoxic in 11 mammalian cell types and effect ...	2011	21818340
class ii enveloped viruses.	a number of viruses transport their genomic material from cell to cell enclosed within a lipid bilayer that is in turn encased within a symmetric protein shell. this review focuses in a group of rna viruses that have this type of virions. this group includes several of important human pathogenic viruses, such as the hepatitis c virus, dengue virus, chikungunya virus, rubella virus, and the bunyaviruses. the best studied are the flaviviruses and the alphaviruses, which have a ß-sheet rich class i ...	2011	21790946
[arborviruses of the california encephalitis serogroup 1n russia and their contribution to infectious pathology].	this review focuses on arborviral infections associated with california serocomplex (bunyaviridae, orthobunyavirus). results of relevant eco-epidemiological and clinical studies in russia are presented suggesting the ubiquitious nature of diseases caused by viruses of the california encephalitis serocomplex (inko, tahyna, snowshoe hare). the etiologic structure of these diseases in taiga and mixed woods of the european part and western siberia is dominated by the inco virus and in southern regio ...	2011	21786596
dc-sign as a receptor for phleboviruses.	during natural transmission, bunyaviruses are introduced into the skin through arthropod bites, and dermal dendritic cells (dcs) are the first to encounter incoming viruses. dc-sign is a c-type lectin highly expressed on the surface of dermal dcs. we found that several arthropod-borne phleboviruses (bunyaviridae), including rift valley fever and uukuniemi viruses, exploit dc-sign to infect dcs and other dc-sign-expressing cells. dc-sign binds the virus directly via interactions with high-mannose ...	2011	21767814
dengue virus seroprevalence among febrile patients in bamako, mali: results of a 2006 surveillance study.	abstract background: dengue viruses (denv) are endemic in over 100 countries worldwide, and annually 50 to 100 million people are infected by one of the four denv serotypes, whereas over 2.5 billion people are at risk for infection. west african countries lack the surveillance to determine the true incidence of dengue; hence, this disease is likely significantly underestimated. in mali, ~14 million people are potentially at risk of acquiring a dengue infection. methods and findings: a serosurvey ...	2011	21767159
gouleako virus isolated from west african mosquitoes constitutes a proposed novel genus in the family bunyaviridae.	the family bunyaviridae is the most diversified family of rna viruses. we describe a novel prototypic bunyavirus, tentatively named goul+®ako virus, isolated from various mosquito species trapped in c+¦te d'ivoire. the s segment comprised 1,087 nucleotides (nt), the m segment 3,188 nt, and the l segment 6,358 nt, constituting the shortest bunyavirus genome known so far. the virus had shorter genome termini than phleboviruses and showed no evidence of encoded nss and nsm proteins. an uncharacteri ...	2011	21715500
human jamestown canyon virus infection --- montana, 2009.	jamestown canyon virus (jcv) is a mosquito-borne zoonotic pathogen belonging to the california serogroup of bunyaviruses. although jcv is widely distributed throughout temperate north america, reports of human jcv infection in the united states are rare. this is the first report of human jcv infection detected in montana, one of only 15 cases reported in the united states since 2004, when jcv became reportable. on may 26, 2009, a man aged 51 years with no travel history outside of montana went t ...	2011	21617630
inhibition of hazara nairovirus replication by small interfering rnas and their combination with ribavirin.	abstract: background: the genus nairovirus in the family bunyaviridae contains 34 tick-borne viruses classified into seven serogroups. hazara virus (hazv) belongs to the crimean-congo hemorrhagic fever (cchf) serogroup that also includes cchf virus (cchfv) a major pathogen for humans. hazv is an interesting model to study cchfv due to a close serological and phylogenetical relationship and a classification which allows handling in a bsl2 laboratory. nairoviruses are characterized by a tripartite ...	2011	21600011
a dna vaccine encoding ubiquitinated rift valley fever virus nucleoprotein provides consistent immunity and protects ifnar(-/-) mice upon lethal virus challenge.	current vaccine candidates against rift valley fever virus (rvfv) incorporate the viral structural glycoproteins as antigens, since triggering antibody responses against them usually correlates with protection. here, we have focused solely on the nucleoprotein of rvfv as a potential target for vaccine development. previous studies in mouse models have already demonstrated that rvfv nucleoprotein can elicit partial protection when administered by means of a dna vaccine or in recombinant, soluble, ...	2011	21549790
molecular epidemiology of oropouche virus, brazil.	oropouche virus (orov) is the causative agent of oropouche fever, an urban febrile arboviral disease widespread in south america, with >30 epidemics reported in brazil and other latin american countries during 1960-2009. to describe the molecular epidemiology of orov, we analyzed the entire n gene sequences (small rna) of 66 strains and 35 partial gn (medium rna) and large rna gene sequences. distinct patterns of orov strain clustered according to n, gn, and large gene sequences, which suggests ...	2011	21529387
[biological and phylogenetic analysis of first isolate of tahyna virus in china].	in 2006, the first chinese tahyna virus isolate (xj0625) was obtained in xinjiang province and human infection were found in the same region. in this study, cell culture, animal experiments, electron microscopy, immunofluorescence assay and cross neutralization tests were performed to see the cell susceptibility, animal pathogenicity, morphology and antigenic and other biological characteristics of xj0625. in addition, molecular biology software was used to analyze the characteristics of molecul ...	2011	21528532
discovery and initial analysis of novel viral genomes in the soybean cyst nematode.	nematodes are the most abundant multi-cellular animals on earth, yet little is known about their natural viral pathogens. to date, only two nematode virus genomes have been reported. consequently, nematode viruses have been overlooked as important biotic factors in the study of nematode ecology. here we show that one plant parasitic nematode species, heterodera glycines, the soybean cyst nematode (scn), harbors four different rna viruses. the nematode virus genomes were discovered in the scn tra ...	2011	21490246
identification of a phylogenetically distinct orthobunyavirus from group c.	apeu virus (apeuv) (family bunyaviridae, genus orthobunyavirus) was plaque purified and characterised by serological and molecular analysis. neutralising assays confirmed cross-reactivity between purified apeuv clones and the caraparu virus complex of group c orthobunyaviruses. partial sequencing of the l, m and s segments of one apeuv clone (apeuv-cl5) was carried out. a phylogenetic tree constructed with the l amino acid sequences clustered apeuv-cl5 within the genus orthobunyavirus, confirmin ...	2011	21465087
[hemorrhagic fever caused by a novel tick-borne bunyavirus in huaiyangshan, china.]	background: from april to july in 2009 and 2010, unexplained severe hemorrhagic fever-like illnesses occurred in farmers from the huaiyangshan mountains range. methods: clinical specimens (blood, urine, feces, and throat swabs) from suspected patients were obtained and stored. mosquitoes and ticks in affected regions were collected. virus was isolated from 2 patients and characterized by whole genome sequencing. virus detection in additional patients and arthropods was done by virus-specific rev ...	2011	21457654
medical microbiology	bunyaviridae is a family of arthropod-borne or rodent-borne, spherical, enveloped rna viruses. bunyaviruses are responsible for a number of febrile diseases in humans and other vertebrates. they have either a rodent host or an arthropod vector and a vertebrate host.	1996	21413286
development of inactivated trivalent vaccine for the teratogenic aino, akabane and chuzan viruses.	aino, akabane and chuzan viruses are arthropod-borne (arbo) viruses transmitted by blood-sucking insects like mosquitoes and culicoides biting midges. these arbovirus infections are mainly associated with abortion, stillbirth and congenital defects in pregnant cattle, sheep and goats, which induces a considerable economic loss in livestock industry. the viruses seem to be widely distributed in southeast asia and australia. as a control strategy, an inactivated trivalent vaccine against aino, aka ...	2011	21411335
truly emerging--a new disease caused by a novel virus.		2011	21410394
fever with thrombocytopenia associated with a novel bunyavirus in china.	heightened surveillance of acute febrile illness in china since 2009 has led to the identification of a severe fever with thrombocytopenia syndrome (sfts) with an unknown cause. infection with anaplasma phagocytophilum has been suggested as a cause, but the pathogen has not been detected in most patients on laboratory testing.	2011	21410387
genomic and phylogenetic characterization of leanyer virus, a novel orthobunyavirus isolated in northern australia.	leanyer virus (leav), currently classified as a member of the genus orthobunyavirus, in the family bunyaviridae, was originally isolated from a pool of anopheles meraukensis mosquitoes, collected at leanyer, northern territory, australia in 1974. when it failed to react in serological tests with antisera from other known viruses, full-length genomic sequencing was pursued to determine the relationship of leav to other orthobunyavirus species. genetic and serological characterization confirmed it ...	2011	21402599
development of a flow-trough microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of european bunyaviruses.	it is suspected that apart from tick-borne encephalitis virus several additional european arboviruses such as the sandfly borne toscana virus, sandfly fever sicilian virus and sandfly fever naples virus, mosquito-borne tahyna virus, inkoo virus, batai virus and tick-borne uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in europe. currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. in order to ...	2011	21390485
a natural m rna reassortant arising from two species of plant- and insect-infecting bunyaviruses and comparison of its sequence and biological properties to parental species.	reassortment allows multicomponent viruses to exchange genome segments, a process well-documented in the vertebrate- and arthropod-infecting members of the family bunyaviridae but not between distinct species of the plant- and insect-infecting members of the genus tospovirus. genome sequence comparisons of a virus causing severe tospovirus-like symptoms in florida tomato with groundnut ringspot virus (grsv) and tomato chlorotic spot virus (tcsv) demonstrated that reassortment has occurred, with ...	2011	21382631
arenaviruses and hantaviruses: from epidemiology and genomics to antivirals.	the arenaviruses and hantaviruses are segmented genome rna viruses that are hosted by rodents. due to their association with rodents, they are globally widespread and can infect humans via direct or indirect routes of transmission, causing considerable human morbidity and mortality. nevertheless, despite their obvious and emerging importance as pathogens, there are currently no effective antiviral drugs (except ribavirin which proved effective against lassa virus) with which to treat humans infe ...	2011	21356244
validation of assays to monitor immune responses in the syrian golden hamster (mesocricetus auratus).	the syrian golden hamster (mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and sars-coronavirus. a lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. the objective of this study was to establish and validate assays to monitor host immune response ...	2011	21334343
mechanism of tripartite rna genome packaging in rift valley fever virus.	the bunyaviridae family includes pathogens of medical and veterinary importance. rift valley fever virus (rvfv), a member in the phlebovirus genus of the family bunyaviridae, is endemic to sub-saharan africa and causes a mosquito-borne disease in ruminants and humans. viruses in the family bunyaviridae carry a tripartite, single-stranded, negative-sense rna genome composed of l, m, and s rnas. little is known about how the three genomic rna segments are copackaged to generate infectious bunyavir ...	2010	21187405
isolation and characterization of sylvatic mosquito-borne viruses in trinidad: enzootic transmission and a new potential vector of mucambo virus.	mosquito surveillance was carried out in three forested regions of trinidad during july 2007-march 2009. a total of 185,397 mosquitoes representing at least 46 species was collected, divided into pools of 1-50 mosquitoes according to species and sex, and screened for arboviruses using cytopathic effect assays on vero cell monolayers. eighty-five viruses were isolated, including members of the genera alphavirus (mucambo virus; mucv) and orthobunyavirus (caraparu, oriboca, bimiti, and wyeomyia vir ...	2010	21118932
concurrent dengue and malaria in the amazon region.	the amazon region has extensive forested areas and natural ecosystems, providing favorable conditions for the existence of innumerous arboviruses. over 200 arboviruses have been isolated in brazil and about 40 are associated with human disease. four out of 40 are considered to be of public health importance in brazil: dengue viruses (1-4), oropouche, mayaro and yellow fever. along with these viruses, about 98% of the malaria cases are restricted to the legal amazon region.	2010	21085859
structural studies of hantaan virus.	hantaan virus is the prototypic member of the hantavirus genus within the family bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. the bunyaviridae are a family of negative-sense rna viruses with three-part segmented genomes. virions are enveloped and decorated with spikes derived from a pair of glycoproteins (gn and gc). here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of hantaan virus virions. we ...	2010	21068243
amino acid changes within the bunyamwera virus nucleocapsid protein differentially affect the mrna transcription and rna replication activities of assembled ribonucleoprotein templates.	the genome of bunyamwera virus (bunv) comprises three rna segments that are encapsidated by the virus-encoded nucleocapsid (n) protein to form ribonucleoprotein (rnp) complexes. these rnps are the functional templates for rna synthesis by the virus-encoded rna-dependent rna polymerase (rdrp). we investigated the roles of conserved positively charged n-protein amino acids in rna binding, in oligomerization to form model rnps and in generating rnp templates active for both rna replication and mrna ...	2010	20943890
[examination of mosquitoes collected in southern moravia in 2006-2008 tested for arboviruses].	the main goals of the study were to carry out virus isolation attempts on vero cell cultures from mosquitoes collected in southern moravia (breclav district, czech republic) and to identify the isolates using a microtiter virus neutralization test. a total of 9.742 female mosquitoes belonging to 13 species were examined, and three viral strains were isolated, all from the mosquitoes collected in 2006: two of these isolates were identified as tahyna orthobunyavirus (both obtained from aedes vexan ...	2010	20925246
bunyaviridae rna polymerases (l-protein) have an n-terminal, influenza-like endonuclease domain, essential for viral cap-dependent transcription.	bunyaviruses are a large family of segmented rna viruses which, like influenza virus, use a cap-snatching mechanism for transcription whereby short capped primers derived by endonucleolytic cleavage of host mrnas are used by the viral rna-dependent rna polymerase (l-protein) to transcribe viral mrnas. it was recently shown that the cap-snatching endonuclease of influenza virus resides in a discrete n-terminal domain of the pa polymerase subunit. here we structurally and functionally characterize ...	2010	20862319
guaroa virus infection among humans in bolivia and peru.	guaroa virus (grov) was first isolated from humans in colombia in 1959. subsequent isolates of the virus have been recovered from febrile patients and mosquitoes in brazil, colombia, and panama; however, association of the virus with human disease has been unclear. as part of a study on the etiology of febrile illnesses in peru and bolivia, 14 grov strains were isolated from patients with febrile illnesses, and 3 additional cases were confirmed by igm seroconversion. the prevalence rate of grov ...	2010	20810845
oropouche virus experimental infection in the golden hamster (mesocrisetus auratus).	oropouche virus (orov), of the family bunyaviridae, is the second most frequent arbovirus causing febrile disease in brazil. in spite of this, little is known about pathogenesis of orov infection. this report describes an experimental model of orov in golden hamster (mesocricetus auratus). following subcutaneous inoculation of orov, over 50% of the animals developed disease characterized by lethargy, ruffled fur, shivering, paralysis, and approximately one third died. animals were sacrificed on ...	2011	20727376
arboviral etiologies of acute febrile illnesses in western south america, 2000-2007.	arthropod-borne viruses (arboviruses) are among the most common agents of human febrile illness worldwide and the most important emerging pathogens, causing multiple notable epidemics of human disease over recent decades. despite the public health relevance, little is know about the geographic distribution, relative impact, and risk factors for arbovirus infection in many regions of the world. our objectives were to describe the arboviruses associated with acute undifferentiated febrile illness ...	2010	20706628
oligomerization of uukuniemi virus nucleocapsid protein.	uukuniemi virus (uukv) belongs to the phlebovirus genus in the family bunyaviridae. as a non-pathogenic virus for humans uukv has served as a safe model bunyavirus in a number of studies addressing fundamental questions such as organization and regulation of viral genes, genome replication, structure and assembly. the present study is focused on the oligomerization of the uukv nucleocapsid (n) protein, which plays an important role in several steps of virus replication. the aim was to locate the ...	2010	20698970
la crosse virus neuroinvasive disease - missouri, 2009.	la crosse virus (lacv), a california serogroup bunyavirus, is a leading cause of pediatric arboviral encephalitis in the united states and is transmitted primarily by the eastern treehole mosquito (aedes triseriatus). on august 7, 2009, the missouri department of health and senior services (mdhss) was notified of suspected lacv neuroinvasive disease in a boy aged 8 years from northwest missouri. laboratory testing at cdc confirmed lacv infection. an environmental inspection identified multiple v ...	2010	20651642
detection of saint louis encephalitis virus in dengue-suspected cases during a dengue 3 outbreak.	arboviruses are frequently associated with outbreaks in humans and represent a serious public health problem. among the brazilian arboviruses, mayaro virus, dengue virus (denv), yellow fever virus, rocio virus, saint louis encephalitis virus (slev), and oropouche virus are responsible for most of human cases. all these arboviruses usually produce undistinguishable acute febrile illness, especially in the acute phase of infection. in this study we investigated the presence of arboviruses in sera ...	2011	20645866
genetic characterization of aino and peaton virus field isolates reveals a genetic reassortment between these viruses in nature.	sequence determination and phylogenetic analysis were conducted using the s, m and l rna segments of the 10 aino, 6 peaton and 1 sango virus (ainov, peav and sanv) field isolates of the genus orthobunyavirus in the family bunyaviridae, respectively. the japanese ainov strains were genetically stable, but the sequence differences between the japanese and australian ainov strains were considerably larger than those among the japanese ainov strains. a similar result was found in the genetic relatio ...	2010	20600386
visualizing the replication cycle of bunyamwera orthobunyavirus expressing fluorescent protein-tagged gc glycoprotein.	the virion glycoproteins gn and gc of bunyamwera virus (bunv), the prototype of the bunyaviridae family and also of the orthobunyavirus genus, are encoded by the medium (m) rna genome segment and are involved in both viral attachment and entry. after their synthesis gn and gc form a heterodimer in the endoplasmic reticulum (er) and transit to the golgi compartment for virus assembly. the n-terminal half of the gc ectodomain was previously shown to be dispensable for virus replication in cell cul ...	2010	20573824
entry of bunyaviruses into mammalian cells.	the bunyaviridae constitute a large family of enveloped animal viruses, many members of which cause serious diseases. however, early bunyavirus-host cell interactions and entry mechanisms remain largely uncharacterized. investigating uukuniemi virus, a bunyavirus of the genus phlebovirus, we found that virus attachment to the cell surface was specific but inefficient, with 25% of bound viruses being endocytosed within 10 min, mainly via noncoated vesicles. the viruses entered rab5a+ early endoso ...	2010	20542252
dynamin-like mxa gtpase: structural insights into oligomerization and implications for antiviral activity.	the interferon-inducible mxa gtpase is a key mediator of cell-autonomous innate immunity against a broad range of viruses such as influenza and bunyaviruses. mxa shares a similar domain structure with the dynamin superfamily of mechanochemical enzymes, including an n-terminal gtpase domain, a central middle domain, and a c-terminal gtpase effector domain. recently, crystal structures of a gtpase domain dimer of dynamin 1 and of the oligomerized stalk of mxa (built by the middle and gtpase effect ...	2010	20538602
mosquito (diptera: culicidae) surveillance for arboviruses in an area endemic for west nile (lineage rabensburg) and tahyna viruses in central europe.	six viral isolates were obtained from 23,243 female mosquitoes (examined in 513 pools) belonging to 16 species and collected along the lower reaches of the dyje river in south moravia (czech republic, central europe) during 2006-2008: five isolates of orthobunyavirus tahyna (tahv, california group, family bunyaviridae: three isolations from aedes vexans (meigen), one from ae. sticticus (meigen), one from culex modestus ficalbi); and one isolation of flavivirus west nile (wnv, japanese encephalit ...	2010	20496595
the n-terminus of bunyamwera orthobunyavirus nss protein is essential for interferon antagonism.	bunyamwera virus nss protein is involved in the inhibition of cellular transcription and the interferon (ifn) response, and it interacts with the med8 component of mediator. a spontaneous mutant of a recombinant nss-deleted bunyamwera virus (rbundelnss2) was identified and characterized. this mutant virus, termed mbunnss22, expresses a 21 aa n-terminally truncated form of nss. like rbundelnss2, mbunnss22 is attenuated in ifn-deficient cells, and to a greater extent in ifn-competent cells. both r ...	2010	20427562
unpaired 5' ppp-nucleotides, as found in arenavirus double-stranded rna panhandles, are not recognized by rig-i.	arenavirus and bunyavirus rna genomes are unusual in that they are found in circular nucleocapsids, presumably due to the annealing of their complementary terminal sequences. moreover, arenavirus genome synthesis initiates with gtp at position +2 of the template rather than at the precise 3' end (position +1). after formation of a dinucleotide, 5' pppgpc(oh) is then realigned on the template before this primer is extended. the net result of this "prime and realign" mechanism of genome initiation ...	2010	20400512
molecular identification of host feeding patterns of snow-melt mosquitoes (diptera: culicidae): potential implications for the transmission ecology of jamestown canyon virus.	we collected blood-fed, snow-melt mosquitoes (culicidae: culiseta and aedes) to describe the feeding patterns of potential mosquito vectors of jamestown canyon virus (jcv, bunyaviridae: orthobunyavirus). jcv is an arthropod-borne, zoonotic virus with deer as the primary amplifying host in western alpine ecosystems. we collected mosquitoes from natural resting areas, fiber pots, and carbon-dioxide baited miniature light traps in the colorado rocky mountains in 2007. we conducted two polymerase ch ...	2010	20380304
detection of flaviviruses and orthobunyaviruses in mosquitoes in the yucatan peninsula of mexico in 2008.	a total of 191,244 mosquitoes from 24 species were collected in the yucatan peninsula of mexico from january to december 2008, and tested for the presence of cytopathic virus by virus isolation in vero cells. eighteen virus isolates were obtained, all of which were orthobunyaviruses. these were identified by reverse transcription-polymerase chain reaction (rt-pcr) and nucleotide sequencing as cache valley virus (n=17) and south river virus (n=1). a subset (n=20,124) of culex quinquefasciatus col ...	2010	20370430
experimental infections of oryzomys couesi with sympatric arboviruses from mexico.	coues rice rat (oryzomys couesi), a species abundant throughout central america, was evaluated experimentally for the ability to serve as an amplifying host for three arboviruses: patois (bunyaviridae, orthobunyavirus), nepuyo (orthobunyavirus), and venezuelan equine encephalitis virus subtype id (togaviridae, alphavirus). these three viruses have similar ecologies and are known to co-circulate in nature. animals from all three cohorts survived infection and developed viremia with no apparent si ...	2010	20134016
a broad-spectrum antiviral targeting entry of enveloped viruses.	we describe an antiviral small molecule, lj001, effective against numerous enveloped viruses including influenza a, filoviruses, poxviruses, arenaviruses, bunyaviruses, paramyxoviruses, flaviviruses, and hiv-1. in sharp contrast, the compound had no effect on the infection of nonenveloped viruses. in vitro and in vivo assays showed no overt toxicity. lj001 specifically intercalated into viral membranes, irreversibly inactivated virions while leaving functionally intact envelope proteins, and inh ...	2010	20133606
nucleotide variability of tahyna virus (bunyaviridae, orthobunyavirus) small (s) and medium (m) genomic segments in field strains differing in biological properties.	tahyna virus (tahv), a mosquito-borne bunyavirus (california group), is frequently associated with inapparent or influenza-like (valtice fever) infections in humans, rarely leading to atypical pneumonia or meningitis. field tahv strains exhibit a high variability in their biological properties with respect to virulence for laboratory mouse, temperature-sensitivity or character of plaques in cell culture. in consideration of the variations in the antigenic properties tahv and its potential geneti ...	2010	20093153
apoptosis induced by oropouche virus infection in hela cells is dependent on virus protein expression.	oropouche (orov) is a single-stranded rna arbovirus of the family bunyaviridae, genus orthobunyavirus, which has caused over half a million cases of febrile illness in brazil in the past 30 years. orov fever has been registered almost exclusively in the amazon region, but global warming, deforestation and redistribution of vectors and animal reservoirs increases the risk of oropouche virus emergence in other areas. orov causes a cytolytical infection in cultured cells with characteristic cytopat ...	2010	20080135
arrival and establishment of aedes japonicus japonicus (diptera: culicidae) in iowa.	the arrival and establishment of aedes (finlaya) japonicus japonicus (theobald) (diptera: culicidae) in iowa are reported. in total, 518 wild adult specimens were collected through the statewide mosquito and mosquito-borne virus surveillance program in 2007 and 2008. specimens were collected with new jersey light traps, co2-baited cdc light traps, grass infusion-baited gravid traps, and mosquito magnet traps located in 12 counties in central and eastern iowa specimens were identified morphologic ...	2009	19960671
a serosurvey reveals bosnia and herzegovina as a europe's hotspot in hantavirus seroprevalence.	the extent of hantavirus seroprevalence in the healthy population from bosnia and herzegovina has not yet been investigated; therefore, the aim of this study was to assess the hantavirus seroprevalence in the population from different regions of bosnia and herzegovina and in different risk groups. the serosurvey included 1331 subjects from endemic and non-endemic regions in bosnia and herzegovina. all sera samples were examined using igg elisa, and western blot (bunyavirus igg) tests. hantavirus ...	2010	19951455
complete nucleotide sequence of four rna segments of fig mosaic virus.	the complete sequence of four viral rna segments of fig mosaic virus (fmv) was determined. each of the four rnas comprises a single open reading frame (orf) 7,093, 2,252, 1,490 and 1,472 nucleotides in size, respectively. these orfs encode the following proteins in the order: rna-dependent rna polymerase (p1 264 kda), a putative glycoprotein (p2 73 kda), a putative nucleocapsid protein (p3 35 kda) and a protein with unknown function (p4 40.5 kda). all rna segments possess untranslated regions co ...	2009	19777155
tensaw virus genome sequence and its relation to other bunyaviridae.	tensaw virus (tsv) belongs to the genus orthobunyavirus within the bunyaviridae family. although tsv does not cause hemorrhagic fever as some other members of its family, serological studies have shown that serum from florida residents react against tsv indicating viral infection in humans. in this study, the three rna genome segments of a tsv isolated from anopheles crucians mosquitoes collected in north central florida in 2006 and a tsv isolate obtained from the cdc, fort collins, were sequenc ...	2009	19760176
the interferon response circuit in antiviral host defense.	viruses have learned to multiply in the face of a powerful innate and adaptive immune response of the host. they have evolved multiple strategies to evade the interferon (ifn) system which would otherwise limit virus growth at an early stage of infection. ifns induce the synthesis of a range of antiviral proteins which serve as cell-autonomous intrinsic restriction factors. for example, the dynamin-like mxa gtpase inhibits the multiplication of influenza and bunyaviruses (such as la crosse virus ...	2009	19739399
genetic analysis of akabane virus isolates from cattle in korea.	bayesian inference (bi) and neighbor joining (nj) analyses of the phylogenetic relationships between the nucleotide sequences of the n gene of akabane virus revealed an unclear topology among genogroups i-iii, which was probably caused by genetic reassortment or recombination between these genogroups. in contrast, nucleotide and amino acid phylogenetic tree analyses of the m rna segment agreed with the topologies obtained by using the bi and nj methods. therefore, distinct genogrouping of akaban ...	2010	19733454
mutational analysis of the bunyamwera orthobunyavirus nucleocapsid protein gene.	the bunyavirus nucleocapsid protein, n, is a multifunctional protein that encapsidates each of the three negative-sense genome segments to form ribonucleoprotein complexes that are the functional templates for viral transcription and replication. in addition, n protein molecules interact with themselves to form oligomers, with the viral l (rna polymerase) protein, with the carboxy-terminal regions of either or both of the virion glycoproteins, and probably also with host cell proteins. bunyamwer ...	2009	19710139
duration of maternally derived antibodies against akabane virus in calves: survival analysis.	to prevent encephalomyelitis caused by akabane virus, as observed in 2006, vaccination of calves is one of the most effective prophylactic measures. for vaccination of calves, the duration of the maternal antibodies need to be considered because these antibodies are an obstacle to the effectiveness of the vaccine. in order to estimate the age of antibody decay in calves and to find factors influencing the duration of passive immunity, we conducted survival analysis using data from nationwide sen ...	2009	19652478
complete nucleotide sequences of the small and medium rna genome segments of kairi virus (family bunyaviridae).	we determined the complete nucleotide sequences of the small (s) and medium (m) rna genome segments of a kairi virus (kriv) isolate from the yucatan peninsula of mexico. the s segment consists of 992 nucleotides, and the m segment consists of 4,619 nucleotides. phylogenetic analyses were conducted on each genomic segment, and these data are discussed. a 526 nucleotide region of the large (l) segment was also sequenced. this is the first study to present sequence and phylogenetic data for a kriv ...	2009	19649762
bunyaviruses and climate change.	it is generally accepted that the planet is undergoing climatic changes, and 'climate change' has become the scapegoat for many catastrophes, including infectious disease outbreaks, as acknowledged by randolph and ergonul, who state 'climate change is the current ubiquitous explanation for increased incidence of infections of many sorts' (future virology 2008; 3: 303-306). however, as these authors argue, this is a highly simplistic view and, indeed, there is a complex network of factors that ar ...	2009	19604275
temporal and geographic evidence for evolution of sin nombre virus using molecular analyses of viral rna from colorado, new mexico and montana.	all viruses in the family bunyaviridae possess a tripartite genome, consisting of a small, a medium, and a large rna segment. bunyaviruses therefore possess considerable evolutionary potential, attributable to both intramolecular changes and to genome segment reassortment. hantaviruses (family bunyaviridae, genus hantavirus) are known to cause human hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. the primary reservoir host of sin nombre virus is the deer mouse (peromyscus ...	2009	19602267
functional analysis of the bunyamwera orthobunyavirus gc glycoprotein.	the virion glycoproteins gn and gc of bunyamwera orthobunyavirus (family bunyaviridae) are encoded by the m rna genome segment and have roles in both viral attachment and membrane fusion. to investigate further the structure and function of the gc protein in viral replication, we generated 12 mutants that contain truncations from the n terminus. the effects of these deletions were analysed with regard to golgi targeting, low ph-dependent membrane fusion, infectious virus-like particle (vlp) form ...	2009	19570952
consensus amplification and novel multiplex sequencing method for s segment species identification of 47 viruses of the orthobunyavirus, phlebovirus, and nairovirus genera of the family bunyaviridae.	a reverse transcription-pcr (rt-pcr) assay was designed, according to previously determined and newly derived genetic data, to target s genomic segments of 47 viruses, including 29 arthropod-borne human pathogens, of the family bunyaviridae. the analytical sensitivity of the presented assay was evaluated through its application to rnas extracted from quantitated dilutions of bunyaviruses of interest. additionally, the assay's analytical specificity was determined through the evaluation of rnas e ...	2009	19535518
serological examination of songbirds (passeriformes) for mosquito-borne viruses sindbis, tahyna, and batai in a south moravian wetland (czech republic).	a total of 178 free-living birds of 14 species of 7 families of passeriformes sampled in a freshwater reedswamp habitat in southern moravia in july 2006 were examined for hemagglutination-inhibiting (hi) antibodies to alphavirus sindbis (sinv), and bunyaviruses tahyna (tahv) and batai (batv). hemagglutination-inhibiting antibody was detected against all three viruses, but at different frequencies: sinv 0.7%, tahv 14.0%, and batv 6.8%. the survey indicates circulation of mosquito-borne viruses ta ...	2009	19534626
infection by bunyamwera virus (orthobunyavirus) in free ranging birds of cordoba city (argentina).	infection by bunyamwera virus (bunv) species (family bunyaviridae, genus orthobunyavirus) has been detected in ewes, goats, cows, horses and humans all the way through america. however, there are no evidences of such infection in birds. to detect bunv (cbaar-426) infection in free ranging birds, a serological survey was carried out in córdoba city between 2004 and 2005. birds of 13 families presented neutralizing antibodies against bunv (cbaar-426), showing an increase in the seroprevalence from ...	2009	19501478
surveillance of mosquito-borne viruses in alberta using reverse transcription polymerase chain reaction with generic primers.	mosquitoes collected during 2003, 2004, and 2005 in alberta, canada, were screened for the presence of a wide range of arboviruses by reverse transcription-polymerase chain reaction (rt-pcr). nucleic acid extracts from mosquito slurries were amplified using universal primers designed to detect viruses belonging to the flavivirus genus of the flaviviridae family and california and bunyamwera serogroups of the bunyavirus genus within the bunyaviridae family. species-specific detection of western e ...	2009	19496438
viruses in the anopheles a, anopheles b, and tete serogroups in the orthobunyavirus genus (family bunyaviridae) do not encode an nss protein.	viruses in the genus orthobunyavirus, family bunyaviridae, have a genome comprising three segments (called l, m, and s) of negative-sense rna. serological studies have classified the >170 named virus isolates into 18 serogroups, with a few additional as yet ungrouped viruses. until now, molecular studies and full-length s-segment nucleotide sequences were available for representatives of eight serogroups; in all cases, the s segment encodes two proteins, n (nucleocapsid) and nss (nonstructural), ...	2009	19439468
t-705 (favipiravir) and related compounds: novel broad-spectrum inhibitors of rna viral infections.	a series of pyrazinecarboxamide derivatives t-705 (favipiravir), t-1105 and t-1106 were discovered to be candidate antiviral drugs. these compounds have demonstrated good activity in treating viral infections in laboratory animals caused by various rna viruses, including influenza virus, arenaviruses, bunyaviruses, west nile virus (wnv), yellow fever virus (yfv), and foot-and-mouth disease virus (fmdv). treatment has in some cases been effective when initiated up to 5-7 days after virus infectio ...	2009	19428599
bunyavirus n: eif4f surrogate and cap-guardian.	hantaviruses comprise a genus of the bunyavirus family of viruses. viruses of this family, along with the arenaviruses, and the orthomyxoviruses, including influenza, contain a negative sense, segmented rna genome. viral nucleocapsid proteins play a well-established role in the formation of intracellular and virion-associated nucleocapsids that harbor and shield viral genomic rna. however, recent observations indicate that hantavirus nucleocapsid protein (n) has additional unexpected biological ...	2009	19342890
single-particle cryo-electron microscopy of rift valley fever virus.	rift valley fever virus (rvfv; bunyaviridae; phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. we report a three-dimensional reconstruction of rvfv vaccine strain mp-12 (rvfv mp-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. although the genomic core of rvfv mp-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and ar ...	2009	19304307
sandfly - pappataci fever in bosnia and herzegovina: the new-old disease.	sandfly fever viruses (sfv) are endemic in the mediterranean, middle east, northern african and western asian countries. toscana virus (tosv), serotype of sandfly fever naples virus, is among of the three most prevalent viruses associated with meningitis during the warm seasons in northern mediterranean countries. the historical data of the sandfly fever (pappataci fever) indicates its origin in bosnia and herzegovina at the end of 19th century. there is a long period of time for which there are ...	2009	19284393
a multipartite single-stranded negative-sense rna virus is the putative agent of fig mosaic disease.	several dsrna bands (approx. 0.6-7 kbp in size) were recovered from tissues of mosaic-diseased fig seedlings which contained the enveloped round structures known as double membrane bodies (dmbs). blast analysis of a 4353 and a 1120 nt sequence from the two largest rna segments showed homology with the polymerase and the putative glycoprotein precursor genes of negative-sense single-stranded rna viruses of the family bunyaviridae. negative- and positive-sense riboprobes designed from both rna seg ...	2009	19264612
nss protein of rift valley fever virus induces the specific degradation of the double-stranded rna-dependent protein kinase.	rift valley fever virus (rvfv) continues to cause large outbreaks of acute febrile and often fatal illness among humans and domesticated animals in africa, saudi arabia, and yemen. the high pathogenicity of this bunyavirus is mainly due to the viral protein nss, which was shown to prevent transcriptional induction of the antivirally active type i interferons (alpha/beta interferon [ifn-alpha/beta]). viruses lacking the nss gene induce synthesis of ifns and are therefore attenuated, whereas the n ...	2009	19211744
bunyamwera orthobunyavirus s-segment untranslated regions mediate poly(a) tail-independent translation.	the mrnas of bunyamwera virus (bunv), the prototype of the bunyaviridae family, possess a 5' cap structure but lack a 3' poly(a) tail, a common feature of eukaryotic mrnas that greatly enhances translation efficiency. viral mrnas also contain untranslated regions (utrs) that flank the coding sequence. using model virus-like mrnas that harbor the renilla luciferase reporter gene, we found that the 3' utr of the bunv small-segment mrna mediated efficient translation in the absence of a poly(a) tai ...	2009	19193790
sporadic oropouche virus infection, acre, brazil.		2009	19193295
an alphavirus replicon-derived candidate vaccine against rift valley fever virus.	rift valley fever virus (rvfv) is a mosquito-transmitted bunyavirus (genus phlebovirus) associated with severe disease in livestock and fatal encephalitis or haemorrhagic fever in a proportion of infected humans. although live attenuated and inactivated vaccines have been used in livestock, and on a limited scale in humans, there is a need for improved anti-rvfv vaccines. towards this goal, sindbis virus replicon vectors expressing the rvfv gn and gc glycoproteins, as well as the non-structural ...	2009	19171081
investigating the specificity and stoichiometry of rna binding by the nucleocapsid protein of bunyamwera virus.	bunyamwera virus (bunv) is the prototypic member of both the orthobunyavirus genus and the bunyaviridae family of negative stranded rna viruses. in common with all negative stranded rna viruses, the bunv genomic and anti-genomic strands are not naked rnas, but instead are encapsidated along their entire lengths with the virus-encoded nucleocapsid (n) protein to form a ribonucleoprotein (rnp) complex. this association is critical for the negative strand rna virus life cycle because only rnps are ...	2009	19168749
encephalomyelitis of cattle caused by akabane virus in southern japan in 2006.	six calves, aged between 55 days and 15 months, were presented between september and november 2006 with neurological signs including limb weakness and circling. microscopical examination of the brain and spinal cord revealed the presence of non-suppurative encephalitis in all animals. perivascular cuffing of lymphocytes and macrophages and diffuse gliosis was prominent in the cerebrum and degeneration and/or necrosis of neurons with vacuolation of the neuropil was present in the brainstem. neuro ...	2009	19162275
vaccination with virus-like particles protects mice from lethal infection of rift valley fever virus.	rift valley fever virus (rvfv) regularly accounts for severe and often lethal outbreaks among livestock and humans in africa. safe and effective veterinarian and human vaccines are highly needed. we present evidence that administration of rvf virus-like particles (vlps) induces protective immunity in mice. in an accompanying paper, (habjan, m., penski, n., wagner, v., spiegel, m., overby, a.k., kochs, g., huiskonen, j., weber, f., 2009. efficient production of rift valley fever virus-like partic ...	2009	19157482