Publications

naturally occurring major and minor capsid protein variants of human papillomavirus 45 (hpv45): differential recognition by cross-neutralizing antibodies generated by hpv vaccines.	we investigated naturally occurring variation within the major (l1) and minor (l2) capsid proteins of human papillomavirus genotype 45 (hpv45). pseudoviruses (psvs) representing hpv45 sublineages a1, a2, a3, b1, and b2 exhibited comparable particle-to-infectivity ratios and morphologies but demonstrated both increased (a2, a3, and b1) and decreased (b2) sensitivities to cross-neutralization by hpv vaccine antibodies compared to that of the a1 sublineage. mutant psvs identified hi loop residue 35 ...	2015	26719255
unique human papillomavirus-type distribution in south african women with invasive cervical cancer and the effect of human immunodeficiency virus infection.	cervical cancer is the most common cause of cancer-related deaths among south african women. viral types associated with cervical cancer may differ not only between countries and regions, but possibly also between human immunodeficiency virus (hiv)-infected and noninfected women.	2015	25950128
human papillomavirus 45 genetic variation and cervical cancer risk worldwide.	human papillomavirus 45 (hpv45) is a member of the hpv18-related alpha-7 species and accounts for approximately 5% of all cervical cancer cases worldwide. this study evaluated the genetic diversity of hpv45 and the association of hpv45 variants with the risk of cervical cancer by sequencing the entire e6 and e7 open reading frames of 300 hpv45-positive cervical samples from 36 countries. a total of 43 hpv45 sequence variants were identified that formed 5 phylogenetic sublineages, a1, a2, a3, b1, ...	2014	24501412
cervical adenocarcinoma in situ recurring as vaginal adenocarcinoma 16 years after hysterectomy.	we report a case in which a vaginal adenocarcinoma was discovered in a 67-year-old woman 16 years after hysterectomy for cervical adenocarcinoma in situ. both the vaginal and cervical lesions exhibited morphologic and immunohistochemical (cdx2-positive) features of intestinal differentiation. linear array human papillomavirus genotyping demonstrated the vaginal adenocarcinoma to contain human papillomavirus 45. we believe the vaginal adenocarcinoma to be related to the cervical adenocarcinoma in ...	2009	19620950
distribution of human papillomavirus genotypes in archival cervical lesions in eastern inner mongolian autonomous region, china.	human papillomavirus (hpv) is the main etiologic factor for cervical cancer (cc). to investigate the prevalence of hpv types in archival cc and its precursors collected form tongliao area, which is located in the east of inner mongolian autonomous region, china, and compare the genotype distribution of hpv in cervical lesions between han chinese and mongolian.	2009	19574785
human papillomavirus type 45 e7 is a transforming protein inducing retinoblastoma protein degradation and anchorage-independent cell cycle progression.	high-risk human papillomaviruses (hpv) cause cervical cancer. the biological properties of hpv-45, the third most prevalent high-risk hpv-genotype, are unknown. we demonstrate here that the hpv-45 e7 protein transforms immortalized nih3t3 fibroblasts, while mutations in either the conserved lxcxe sequence (c28g) or the carboxyl-terminus (delta87lqqlf91) significantly abolish this activity. to address the mechanisms underlying cell transformation by hpv-45 e7, we investigated its impact on the ce ...	2008	18649911
metastatic penile squamous cell carcinoma to the retroperitoneum in a man with human papillomavirus type 45.	squamous cell carcinoma of the penis is a rare cancer associated with smoking, genital warts, lack of neonatal circumcision, and human papillomavirus types 16 and 18. metastasis of this condition is even rarer, occurring primarily in the lungs or liver. the current report describes a case of penile squamous cell carcinoma in an uncircumcised 63-year-old man who smoked cigarettes and who had genital warts and human papillomavirus type 45. metastasis occurred in the abdominal cavity.	2008	18587080
human papillomavirus type 45 propagation, infection, and neutralization.	the organotypic (raft) culture system has allowed the study of the entire differentiation-dependent life cycle of human papillomaviruses (hpvs), including virion morphogenesis. we introduced linearized hpv45 genomic dna into primary keratinocytes, where it recircularized and maintained episomally at a range of 10-50 copies of hpv genomic dna. following epithelial stratification and differentiation in organotypic culture, virion morphogenesis occurred. hpv45 virions were purified from raft cultur ...	2003	12890615
nuclear import and dna binding of human papillomavirus type 45 l1 capsid protein.	during the life cycle of human papillomaviruses (hpvs), the l1 capsid proteins seem to enter the nucleus twice: once after the virions infect the cells, and later during the productive phase when they assemble the replicated hpv genomic dna into infectious virions. we established for the high-risk hpv45 that when digitonin-permeabilized hela cells were incubated with l1 homopentameric capsomers, the hpv45 l1 protein was imported into the nucleus in a receptor-mediated manner. in contrast, intact ...	2000	10967550
detection and characterization of human papillomavirus type 45 dna in the cervical carcinoma cell line ms751.	the cervical carcinoma-derived cell line ms751 was examined for human papillomavirus (hpv) dna and rna. a genomic fragment containing both viral and cellular sequences was cloned. sequence analysis showed that ms751 cells contain a partially deleted hpv-45 genome integrated at a single chromosomal site. hpv sequences from the e6-e7 region are expressed as poly(a)+ rna.	1997	9049418
production of human papillomavirus type 45 virus-like particles in insect cells using a recombinant baculovirus.	the l1 major capsid protein of human papillomavirus type 45 was expressed in insect cells using recombinant baculovirus technology. human papillomavirus type 45 l1 major capsid protein self-assembled into empty virus-like particles (vlps). these 50-60 nm diameter particles were present in the nuclei of recombinant baculovirus-infected insect cells and had a density of 1.29 - 1.30 g/cm3 in cesium chloride. the expressed human papillomavirus type 45 l1 protein sequence is identical to the referenc ...	1996	8764516
characterization of human papillomavirus type 45, a new type 18-related virus of the genital tract.	dna of human papillomavirus (hpv) type 45, a new hpv type 18-related papillomavirus of the genital tract, was cloned from a recurrent cervical lesion displaying mild to moderate dysplasia with koilocytosis. hpv-45 dna was identified in paraffin sections of biopsies of both the initial and recurrent lesions of the patient, taken 7 months apart. hpv-45 dna hybridized efficiently to that of many different hpv types under low and moderate stringency conditions (tm - 37 degrees c to tm - 25 degrees c ...	1987	2826649