Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in china. | abstract: | 2011 | 22188676 |
| Genetic characterization of equine adenovirus type 1. | Two known serotypes of equine adenovirus (EAdV), equine adenovirus type 1 (EAdV-1) and equine adenovirus type 2 (EAdV-2) have been isolated from horses. EAdV-1 is predominantly associated with upper respiratory tract infections while EAdV-2 appears to be associated with gastrointestinal infections in horses. In this report the EAdV-1 genome has been sequenced for the first time. The EAdV-1 genome encoded genes are characteristic of the Mastadenovirus genus such as protein V and IX. Unexpectedly, ... | 2011 | 21924564 |
| Persistence and the state of bovine and porcine adenoviral vector genomes in human and nonhuman cell lines. | The state of vector genome in transduced cells influences the duration of transgene expression and can be a safety concern if it gets integrated randomly into the host genome. Although human adenovirus (Ad) serotype 5 (HAd5) mainly persists in a linear episomal form, information regarding the state of bovine Ad serotype 3 (BAd3) and porcine Ad serotype 3 (PAd3) vector genomes in human and nonhuman cells is currently unknown. To address this issue, MDA-MB-231 (human), MDBK (bovine), PK-15 (porcin ... | 2011 | 21864589 |
| isolation of a novel adenovirus from california sea lions zalophus californianus. | viral hepatitis associated with adenoviral infection has been reported in california sea lions zalophus californianus admitted to rehabilitation centers along the california coast since the 1970s. canine adenovirus 1 (cadv-1) causes viral hepatitis in dogs and infects a number of wildlife species. attempts to isolate the virus from previous sea lion hepatitis cases were unsuccessful, but as the hepatitis had morphologic features resembling canine infectious hepatitis, and since the virus has a w ... | 2011 | 21790072 |
| Genomic and phylogenetic analyses of murine adenovirus 2. | Murine adenoviruses (MAdV) are supposedly the oldest members of the genus Mastadenovirus. Currently, there are three distinct MAdV types known with rather different tropism and pathology. Here we report and annotate the DNA sequence of the full genome of MAdV-2. It was found to consist of 35,203bp thus being considerably larger than the genomes of the other two MAdV types. The increased size of the MAdV-2 genome is generally due to larger genes and ORFs, although some differences in the number o ... | 2011 | 21683742 |
| detection of known and novel adenoviruses in cattle wastes using broad-spectrum primers. | critical assessment of bovine adenoviruses (badv) as indicators of environmental fecal contamination requires improved knowledge of their prevalence, shedding dynamics, and genetic diversity. we examined dna extracted from bovine and other animal waste samples collected in wisconsin for atadenoviruses and mastadenoviruses using novel, broad-spectrum pcr primer sets. badv were detected in 13% of cattle fecal samples, 90% of cattle urine samples and 100% of cattle manure samples; forty-four percen ... | 2011 | 21622778 |
| microbiological and pathological examination of fatal calf pneumonia cases induced by bacterial and viral respiratory pathogens. | the infectious origin of fatal cases of calf pneumonia was studied in 48 calves from 27 different herds on postmortem examination. lung tissue samples were examined by pathological, histological, bacterial culture, virus isolation and immunohistochemical methods for the detection of viral and bacterial infections. pneumonia was diagnosed in 47/48 cases and infectious agents were found in 40/47 (85%) of those cases. the presence of multiple respiratory pathogens in 23/40 (57.5%) cases indicated t ... | 2010 | 20713325 |
| host range, prevalence, and genetic diversity of adenoviruses in bats. | bats are the second largest group of mammals on earth and act as reservoirs of many emerging viruses. in this study, a novel bat adenovirus (adv) (btadv-tjm) was isolated from bat fecal samples by using a bat primary kidney cell line. infection studies indicated that most animal and human cell lines are susceptible to btadv-tjm, suggesting a possible wide host range. genome analysis revealed 30 putative genes encoding proteins homologous to their counterparts in most known advs. phylogenetic ana ... | 2010 | 20089640 |
| ovine atadenovirus, a novel and highly immunogenic vector in prime-boost studies of a candidate hiv-1 vaccine. | ovine adenovirus type 7 (oadv) is the prototype member of the genus atadenovirus. no immunity to the virus has so far been detected in human sera. we describe the construction and evaluation of a candidate hiv-1 vaccine based on oadv and its utilisation alone and in combination with plasmid-, human adenovirus type 5 (hadv5; a mastadenovirus)-, and modified vaccinia ankara (mva)-vectored vaccines. all vectors expressed hiva, an immunogen consisting of hiv-1 clade a consensus gag-derived protein c ... | 2009 | 19853074 |
| bovine adenovirus serotype 3 utilizes sialic acid as a cellular receptor for virus entry. | bovine adenovirus serotype 3 (bad3) and porcine adenovirus serotype 3 (pad3) entry into the host cells is independent of coxsackievirus adenovirus receptor and integrins. the role of sialic acid in bad3 and pad3 entry was investigated. removal of sialic acid by neuraminidase, or blocking sialic acid by wheat germ agglutinin lectin significantly inhibited bad3, but not pad3, transduction of madin-darby bovine kidney cells. maackia amurensis agglutinin or sambucus nigra (elder) agglutinin treatmen ... | 2009 | 19646729 |
| adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus. | adeno-associated virus (aav-2) replicates to high titers when host cells are coinfected with a helper virus. here we analyzed the coinfection of aav-2 and mouse adenovirus (mav-1) in murine fibroblasts. we observed that aav-2/mav-1 coinfected nih 3t3 cells produced approximately 10-40-fold less aav-2 dnase resistant particles than hela cells. levels of aav-2 dna replication were approximately 30-fold less in 3t3 cells as compared to hela cells coinfected with human adenovirus (ad-5). a study of ... | 2009 | 19464040 |
| a novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses. | during cell culture isolation experiments to recover dobrava hantavirus from a suspension of liver from a striped field mouse (apodemus agrarius), an unknown virus was coisolated. atypically for hantaviruses, it had extensive cytopathic effects. using a random pcr approach, it was identified as a novel murine adenovirus, madv-3 (for madv type 3). a plaque-purified virus clone was prepared and further characterized. the complete genome sequence of madv-3 was determined to be 30,570 bp in length. ... | 2009 | 19297486 |
| [application of molecular methods in the diagnosis and epidemiological study of viral respiratory infections]. | to date, more than two hundred viruses, belonging to six different taxonomic families, have been associated with human respiratory tract infection. the widespread incorporation of molecular methods into clinical microbiology laboratories has not only led to notable advances in the etiological diagnosis of viral respiratory infections but has also increased insight into the pathology and epidemiological profiles of the causative viruses. because of their high sensitivity, molecular techniques mar ... | 2008 | 19195443 |
| [sensitivity and specificity of the vikia rota-adeno immuno-chromatographic test (biomérieux) and the elisa ideia rotavirus kit (dako) compared to genotyping]. | the performances of two diagnostic tests for rotavirus infection in stool samples were evaluated during a prospective study in children of less than 36 months in child-care centers of lyon from november 2004 to may 2005. the vikia rota-adeno immuno-chromatographic test (biomérieux) and the elisa ideia rotavirus kit (dako) were compared with a referral method, the genotyping. fifty-seven stool samples were collected and analyzed by rt-pcr. the virus genome was detected in 29 samples. the most fre ... | 2009 | 18838230 |
| interaction of bovine adenovirus-3 33k protein with other viral proteins. | the adenovirus 33k protein appears to be a multifunctional protein performing different roles in viral infection. the involvement of 33k protein in different steps of adenovirus replication may require protein-protein interaction. using 33k protein as a bait in the yeast two-hybrid system, we screened open reading frames (orfs) of bovine adenovirus (badv)-3 for potential interactions with 33k protein. interestingly, 33k protein showed specific interaction with 100k and pv proteins. the yeast two ... | 2008 | 18799182 |
| structure of the c-terminal head domain of the fowl adenovirus type 1 short fibre. | there are more than 100 known adenovirus serotypes, including 50 human serotypes. they can infect all 5 major vertebrate classes but only aviadenovirus infecting birds and mastadenovirus infecting mammals have been well studied. celo (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory pathologies in birds. most studies on celo virus have focussed on its genome sequence and organisation whereas the structural work on celo proteins has only recently started. contrary to m ... | 2008 | 18561970 |
| cryoelectron microscopy map of atadenovirus reveals cross-genus structural differences from human adenovirus. | a three-dimensional (3d) cryoelectron microscopy reconstruction of the prototype atadenovirus (oadv [an ovine adenovirus isolate]) showing information at a 10.6-a resolution (0.5 fourier shell correlation) was derived by single-particle analysis. this is the first 3d structure solved for any adenovirus that is not a mastadenovirus, allowing cross-genus comparisons between structures and the assignment of genus-specific capsid proteins. viable oadv mutants that lacked the genus-specific lh3 and p ... | 2008 | 18508893 |
| serologic and hexon phylogenetic analysis of ruminant adenoviruses. | the objectives of this study were to determine the antigenic relationship among ruminant adenoviruses and determine their phylogenetic relationship based on the deduced hexon gene amino acid sequence. results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions among bovine adenovirus type 6 (badv-6), badv-7, ovine adenovirus type 7 (oadv-7), caprine adenovirus type 1 (gadv-1), and deer adenovirus (odocoileus adenovirus 1, odadv-1). no an ... | 2008 | 18340400 |
| restoration of wild-type p53 function in human tumors: strategies for efficient cancer therapy. | the p53 tumor suppressor gene is mutated in around 50% of all human tumors. most mutations inactivate p53's specific dna binding, resulting in failure to activate transcription of p53 target genes. as a consequence, mutant p53 is unable to trigger a p53-dependent biological response, that is cell cycle arrest and apoptosis. many tumors express high levels of nonfunctional mutant p53. several strategies for restoration of wild-type p53 function in tumors have been designed. wild-type p53 reconsti ... | 2007 | 17419952 |
| [molecular biology of adenoviral vectors]. | gene therapy is based on the use of dna as a therapeutic material as an alternative therapeutic tool for treatment of human diseases. all proteins are codified into the dna and several diseases result from the absence or aberrant expression of one or related genes, absence of expression of functional proteins, and alterations for regulation process in transport and degradation mechanisms. in this regard, several diseases could be potentially treated through the expression of the normal form of t ... | 2006 | 17244507 |
| e1a promoter of bovine adenovirus type 3. | conserved motifs of eukaryotic gene promoters, such as tata box and caat box sequences, of e1a of human adenoviruses (e.g human adenovirus 5) lie between the left inverted terminal repeat (itr) and the atg of e1a. however, analysis of the left end of the bovine adenovirus 3 (badv-3) genome revealed that the conserved sequences of the e1a promoter are present only in the itr. as such, the promoter activity of itr was tested in the context of a badv-3 vector or a plasmid-based system. different re ... | 2006 | 17098968 |
| 293t cells expressing simian virus 40 t antigen are semi-permissive to bovine adenovirus type 3 infection. | human cells do not normally support productive bovine adenovirus type 3 (badv-3) infection. here, the outcome of badv-3 infection of both 293 cells and 293 cells modified to constitutively express the simian virus 40 (sv-40) t antigen (293t cells) was studied. whereas badv-3 could efficiently infect 293 cells, there was a block in virus dna replication, late-gene expression and virus production. in contrast, replication and efficient virus production could be detected in 293t cells infected with ... | 2006 | 16528030 |
| detection of antibodies against adenovirus protein ix, fiber, and hexon in human sera by immunoblot assay. | the 51 serotypes of human adenoviruses (hadvs) of the genus mastadenovirus are classified into the six species hadv-a to hadv-f. for the detection of genus- and species-specific antibodies in human sera an immunoblot assay was developed. the recombinant long fiber of hadv-41[f] (ad41fi) and the native hexon of hadv-5[c] were used as genus-specific antigens. the recombinant capsid protein ix (pix) of hadv-2 (ad2pix[c]) and hadv-41 (ad41pix[f]), the c-terminal pix part of hadv-3 (ad3pixc[b]), and ... | 2005 | 16145087 |
| lh3, a "homologue" of the mastadenoviral e1b 55-kda protein is a structural protein of atadenoviruses. | ovine adenovirus serotype 7 (oadv), the prototype atadenovirus, has gene homologues for most mastadenovirus structural proteins but lacks proteins v and ix. instead, oadv has structural proteins of 32 and 42 kda although the gene encoding the latter had not previously been identified. the presently reported studies of oadv virions have now identified a minor structural polypeptide of approximately 40 kda as the product of the l1 52/55-kda gene and, more surprisingly, shown that the 42-kda protei ... | 2005 | 16112161 |
| acute respiratory infection with mouse adenovirus type 1. | studies of the pathogenesis of adenovirus respiratory disease are limited by the strict species-specificity of the adenoviruses. following intranasal inoculation of adult c57bl/6 mice with mouse adenovirus type 1 (mav-1), we detected mav-1 early region 3 (e3) and hexon gene expression in the lungs at 7 days post-infection (dpi). we detected mav-1 e3 protein in the respiratory epithelium at 7 dpi. we did not detect viral mrna or protein at 14 dpi, but mav-1 dna was detected by pcr at 21 dpi. chem ... | 2005 | 16054189 |
| evaluation of promoters for foreign gene expression in the e3 region of bovine adenovirus type-3. | in order to optimize foreign gene expression in the e3 region of badv-3, we constructed full-length badv-3 genomic dna clones containing a reporter gene (truncated glycoprotein gd of bovine herpesvirus 1, gdt), under the control of exogenous promoters inserted in either direction in the e3 region. irrespective of exogenous transcriptional elements, viable recombinant badv-3 viruses could only be isolated when the gdt expression cassettes were inserted in the e3 region parallel to the direction o ... | 2005 | 15845268 |
| the effect of paralogous lineages on the application of reconciliation analysis by cophylogeny mapping. | paralogy defines similarity caused by duplication rather than common descent and is well known in the case of paralogous gene copies within a single genome. the term is here extended to paralogous lineages of associates within a single host. the phylogenies of four genera within the herpesviridae were reconciled with host phylogenies using cophylogenetic mapping. the observed correspondence for each pair of phylogenies was evaluated through randomization of the viral phylogeny and demonstrated t ... | 2005 | 15805015 |
| the coiled-coil domain of the adenovirus type 5 protein ix is dispensable for capsid incorporation and thermostability. | the 14.4-kda hexon-associated protein ix (pix) acts as a cement in the capsids of primate adenoviruses and confers a thermostable phenotype. here we show that deletion of amino acids 100 to 114 of adenovirus type 5 pix, which eliminates the conserved coiled-coil domain, impairs its capacity to self-associate. however, pixdelta100-114 is efficiently incorporated into the viral capsid, and the resulting virions are thermostable. deletion of the central alanine-rich domain, as in pixdelta60-72, doe ... | 2005 | 15709043 |
| comparative transduction efficiencies of human and nonhuman adenoviral vectors in human, murine, bovine, and porcine cells in culture. | clinical usefulness of human ad serotype 5 (had5) based vectors is limited primarily because of preexisting ad immunity and lack of targeting to specific cell types. alternative vectors based on less prevalent had serotypes as well as nonhuman adenoviruses such as porcine ad serotype 3 (pad3) and bovine ad serotype 3 (bad3) are being developed to overcome these shortcomings. using virus neutralization assay, we examined whether preexisting ad immunity in humans would cross-neutralize pad3 or bad ... | 2005 | 15649439 |
| characterization and nuclear localization of the fiber protein encoded by the late region 7 of bovine adenovirus type 3. | to identify the protein encoded by the l7 region of bovine adenovirus-3 (badv-3), specific antisera were raised by immunizing rabbits with bacterial fusion proteins encoding the n-terminus or c-terminus of the badv-3 fiber protein. immunoprecipitation and western blot analysis confirmed that the fiber is expressed as a 102 kda glycoprotein, which is localized to the nucleus of infected cells. to identify the nuclear localization signals (nls), badv-3 fiber deletion mutants and gfp/beta-galactosi ... | 2004 | 15593420 |
| analysis of the first complete genome sequence of an old world monkey adenovirus reveals a lineage distinct from the six human adenovirus species. | simian adenovirus 3 (sadv-3) is one of several adenoviruses that were isolated decades ago from old world monkeys. determination of the complete dna sequence of sadv-3 permitted the first full genomic comparison of a monkey adenovirus with adenoviruses of humans (hadvs) and chimpanzees, which are recognized formally as constituting six of the species (hadv-a to hadv-f) within the genus mastadenovirus. the sadv-3 genome is 34 246 bp in size and has a g+c content of 55.3 mol%. it contains all the ... | 2004 | 15448340 |
| role of bovine adenovirus-3 33k protein in viral replication. | the l6 region of bovine adenovirus type (badv)-3 encodes a nonstructural protein named 33k. to identify and characterize the 33k protein, rabbit polyclonal antiserum was raised against a 33k-gst fusion protein expressed in bacteria. anti-33k serum immunoprecipitated a protein of 42 kda in in vitro translated and transcribed mrna of 33k. however, three proteins of 42, 38, and 33 kda were detected in badv-3 infected cells. to determine the role of this protein in virus replication, a recombinant b ... | 2004 | 15165819 |
| bovine adenovirus type 3 containing heterologous protein in the c-terminus of minor capsid protein ix. | earlier, we detected pix of badv-3 as a 14-kda protein in purified virions. analysis of badv-3 pix using different region antibodies revealed that the n-terminus and central domain of the pix contain immunogenic sites and are not exposed on the surface of badv-3 virion. this suggested that the c-terminus of badv-3 pix (125 amino acid) may be exposed on the virion and may be used as a site for incorporation of heterologous peptides or proteins. we constructed recombinant bav950 containing a small ... | 2004 | 15016551 |
| detection of bovine and porcine adenoviruses for tracing the source of fecal contamination. | in this study, a molecular procedure for the detection of adenoviruses of animal origin was developed to evaluate the level of excretion of these viruses by swine and cattle and to design a test to facilitate the tracing of specific sources of environmental viral contamination. two sets of oligonucleotides were designed, one to detect porcine adenoviruses and the other to detect bovine and ovine adenoviruses. the specificity of the assays was assessed in 31 fecal samples and 12 sewage samples th ... | 2004 | 15006765 |
| dna sequencing and analysis of the right-hand part of the genome of the unique bovine adenovirus type 10. | the prototype strain of bovine adenovirus (badv) type 10 and four additional isolates that were indistinguishable in serum-neutralization tests have been shown to have remarkable variation in their genome size and restriction maps. in the present study, more than 40 % of the dna sequence of the badv-10 isolate with the longest genome was determined. a biased base composition resulting in low (<41 %) gc content was noticed. analysis of the genes of the dna-binding protein, 100k, 33k, pviii and fi ... | 2004 | 14993642 |
| adenovirus-mediated gene delivery: an overview. | adenoviruses, which were first isolated in the 1950s, have been developed as gene-delivery vehicles, or vectors, since the early 1980s. the adenoviruses constitute the adenoviridae family, which is divided into two genera: the aviadenovirus genus infects only birds, whereas the mastadenovirus genus contains viruses that infect a range of mammalian species. human adenoviruses are classified into six subgroups based on the percentage of guanine and cytosine in the dna molecules and the ability to ... | 2004 | 14970581 |
| altered tropism of recombinant bovine adenovirus type-3 expressing chimeric fiber. | recombinant bovine adenovirus-3 (bav-3) has been used as a gene delivery vector for vaccination of calves. however, its usefulness as a vector for non-bovine species is limited due to poor transduction efficiency. to develop bav-3 based vector for non-bovine species, we determined the feasibility of making targeted bav-3 vector by modifying its natural tropism. we constructed a chimeric virus, bav600, in which the knob region of the bav-3 fiber protein was replaced with that from human adenoviru ... | 2004 | 14687941 |
| construction, rescue, and characterization of vectors derived from ovine atadenovirus. | gene transfer vectors derived from ovine atadenovirus type 7 (oadv) can efficiently infect a variety of mammalian cells in vitro and in vivo to deliver and express transgenes. however, early oadv vectors were designed on human mastadenovirus principles prior to the complete characterization of oadv genes and transcripts. the distinctive arrangement of the oadv genome has suggested ways to improve oadv vector design and utility. we therefore developed a cosmid-based approach that allows efficient ... | 2003 | 14581531 |
| identification of cis-acting sequences required for selective packaging of bovine adenovirus type 3 dna. | the assembly of adenovirus particles is a multistep process, in which viral genomic dna is selected and subsequently inserted into preformed empty capsids. the selective encapsidation of the adenovirus genome is directed by cis-acting packaging motifs, termed a repeats due to their at-rich character in dna sequence. a repeats are usually located at the left end of the viral genome. in this report, the construction and analysis of bovine adenovirus type 3 (badv-3) mutants containing deletion muta ... | 2003 | 14573799 |
| isolation of a bovine adenovirus serotype 10 from a calf in the united states. | virus isolated from the lung, liver, kidney, and small intestine of a 3-month-old holstein heifer with a clinical history of pneumonia and lesions in multiple organs was identified as an adenovirus on the basis of morphological and physicochemical characteristics. the adenovirus was determined to be a serotype 10 bovine adenovirus and represents the first reported isolation of this serotype in the united states. inoculation of calves with this isolate resulted in mild to moderate clinical respon ... | 1999 | 12968729 |
| molecular anatomy of tupaia (tree shrew) adenovirus genome; evolution of viral genes and viral phylogeny. | adenoviruses are globally spread and infect species in all five taxons of vertebrates. outstanding attention is focused on adenoviruses because of their transformation potential, their possible usability as vectors in gene therapy and their applicability in studies dealing with, e.g. cell cycle control, dna replication, transcription, splicing, virus-host interactions, apoptosis, and viral evolution. the accumulation of genetic data provides the basis for the increase of our knowledge about aden ... | 2003 | 12913356 |
| new approaches to the prevention and eradication of rabies. | despite significant progress in improving the pre- and postexposure prophylaxis of human rabies, the development of better and more cost-effective vaccines and antiviral therapeutics remains a major goal for the treatment of human rabies, the control of animal rabies and particularly for the eradication of rabies virus reservoirs in terrestrial wildlife. in this review, we discuss the structural requirements for an effective rabies vaccine, as well as new strategies currently in use for the deve ... | 2003 | 12903805 |
| crkiii: a novel and biologically distinct member of the crk family of adaptor proteins. | as the role for adaptor proteins constantly proliferates, appreciation of their importance has never been higher. the crk family of adaptor proteins is no exception. currently comprising four members, v-crk, crki, crkii and crk-like protein, we have introduced a fifth member, crkiii. cloned by the cort technique, crkiii is identical in sequence to crkii until the second of its two sh3 domains, which is disrupted partway through and results in a nonfunctional domain and a unique c-terminal sequen ... | 2003 | 12894221 |
| molecular comparison of the fibre proteins of bovine adenovirus subtype a and subtype b. | the fibre gene of the bovine adenovirus type 2 (badv2) subtype b was prepared for sequencing by using cloning, sub-cloning and pcr amplification techniques. the nucleotide sequence of the total fibre gene was determined, and it was found to consist of 1,647 nucleotides, coding for a polypeptide of 549 amino acids. the fibre gene regions of badv2 a and b subtypes were aligned. the nucleotide identity of the total fibre gene was found to be 60.5%; however, the homology showed great differences in ... | 2003 | 12837557 |
| gene therapy with gm-csf, interleukin-4 and herpes simplex virus thymidine kinase shows strong antitumor effect on lung cancer. | inadequate release of tumor antigens (ta) and a defective antigen presentation by dendritic cells (dc) are the major mechanisms for how tumor cells can escape the host immune surveillance. | 2003 | 12828142 |
| chemo-radio-gene therapy for colorectal cancer cells using escherichia coli uracil phosphoribosyltransferase gene. | 5-fluorouracil (5-fu) is one of the most widely used chemotherapeutic agents, and is known to be a radiosensitizer. previously, we reported that adenoviral transduction of the escherichia coli (e. coli) uracil phosphoribosyltransferase (uprt) gene induced marked sensitivity in human colon cancer cells to 5-fu. the aim of the current study was to investigate the efficacy of virally-directed uprt and 5-fu to enhance the radiosensitivity of ht29 human colon cancer cells. cytotoxicity as a result of ... | 2003 | 12820392 |
| combination efficacy of doxorubicin and adenoviral methioninase gene therapy with prodrug selenomethionine. | we have previously demonstrated an enzyme activation prodrug gene therapy strategy using the methionine alpha,gamma-lyase gene (met) cloned from pseudomonas putida, in combination with selenomethionine (semet) as a prodrug. met gene transfer via a recombinant adenovirus (ad-met) converts the physiologic compound semet to highly toxic methylselenol. in this study, we have developed a combination therapy approach using ad-met/semet gene therapy and doxorubicin (dox). the combination significantly ... | 2003 | 12820369 |
| adenovirus-mediated heat-activated antisense ku70 expression radiosensitizes tumor cells in vitro and in vivo. | ku70 is one component of a protein complex, ku70 and ku80, that functions as a heterodimer to bind dna double-strand breaks and activates dna-dependent protein kinase. our previous study with ku70-/- and ku80-/- mice, and cell lines has shown that ku70- and ku80-deficiency compromises the ability of cells to repair dna double-strand breaks, increases radiosensitivity of cells, and enhances radiation-induced apoptosis. in this study, we examined the feasibility of using adenovirus-mediated, heat- ... | 2003 | 12810658 |
| adenovirus with insertion-mutated e1a selectively propagates in liver cancer cells and destroys tumors in vivo. | the adenovirus e1a proteins are involved in the transcriptional activation of viral and cellular genes needed for controlling cell cycle and virus replication. undifferentiated embryonic carcinoma cells have the ability to produce an e1a-like activity that can induce the expression of e1a-targeted adenoviral and cellular genes in the absence of the e1a products. differentiated embryonic carcinoma cells lose the ability to produce the e1a-like activity. in this study, we investigated the e1a-like ... | 2003 | 12810631 |
| evaluation of virucidal activity of three commercial disinfectants and formic acid using bovine enterovirus type 1 (ecbo virus), mammalian orthoreovirus type 1 and bovine adenovirus type 1. | a modified version of the test method of the comité européen de normalisation (cen) was developed using formic acid and three commercial disinfectants to evaluate virucidal activity against three non-enveloped viruses, bovine enterovirus type 1 (ecbo virus), mammalian orthoreovirus type 1 and bovine adenovirus type 1 (bav 1). determination of the effects of temperature was carried out at 20 and 10 degrees c. all tests with protein load used bovine serum albumin (bsa) and yeast extract. the inves ... | 2003 | 12788019 |
| bovine adenovirus type 3 pneumonia in dexamethasone-treated calves. | the effects of immunosuppression were examined in 1.5-month-old calves that were given dexamethasone (dm) before endobronchial inoculation with bovine adenovirus type 3 (bav-3). immunohistopathologically, severe necrotizing bronchiolitis with eosinophilic and basophilic intranuclear inclusion bodies was observed both in dm-treated 1.5-month-old infected calves and in non-dm-treated 7-day-old infected calves. these inclusion bodies were correlated with the detection of bav-3 antigen and viral par ... | 2003 | 12637751 |
| characterization of the complete genome of the tupaia (tree shrew) adenovirus. | the members of the family adenoviridae are widely spread among vertebrate host species and normally cause acute but innocuous infections. special attention is focused on adenoviruses because of their ability to transform host cells, their possible application in vector technology, and their phylogeny. the primary structure of the genome of tupaia adenovirus (tav), which infects tupaia spp. (tree shrew) was determined. tree shrews are taxonomically assumed to be at the base of the phylogenetic tr ... | 2003 | 12634391 |
| apoptosis in calf pneumonia induced by endobronchial inoculation with bovine adenovirus type 3 (bav-3). | three calves aged 1 week (group 1), three aged 6 weeks (group 2) and three aged 6 weeks (having been pretreated with dexamethasone) (group 3) were infected endobronchially with bovine adenovirus type 3 (bav-3). all calves had received colostrum. the histopathological, immunohistochemical, ultrastructural and tunel features were examined on post-inoculation day (pid) 3, 5 and 7. viral replication and intranuclear inclusions were frequently observed in groups 1 and 3, but not in group 2. the lesio ... | 2003 | 12634091 |
| immuno-gene therapy with adenoviruses expressing fms-like tyrosine kinase 3 ligand and cd40 ligand for mouse hepatoma cells in vivo. | introduction of genes encoding immuno-stimulatory cytokines into cancer cells is known to enhance antitumor immunity. cd40 ligand (cd40l, cd154) and fms-like tyrosine kinase 3 ligand (flt3l) are recently identified cytokines, which have been demonstrated to stimulate antitumor immunity in several cancer models. however little is known about antitumor activity of ftl3l and cd40l against hepatocellular carcinoma (hcc). in the present study, we constructed replication-defective adenoviruses express ... | 2003 | 12527933 |
| a 72-bp internal deletion in the left inverted terminal repeat of the bovine adenovirus type 3 genome does not affect virus replication. | the genome of bovine adenovirus type 3 (bav3) is flanked by 195-base pair (bp) inverted terminal repeats (itr). we isolated a bav3 mutant (bav3c29) having an internal deletion within the left itr. the deletion eliminated 72 bp between nucleotides (nt) 89 and 162, including most of the gc-rich sequences located close to the end of the itr sequences. this deletion did not seem to have any affect on the virus plaque size or morphology and the kinetics of viral replication compared to wild-type (wt) ... | 2002 | 12403925 |
| immunohistopathology of calf pneumonia induced by endobronchial inoculation with bovine adenovirus 3. | three 1-week-old and three 3-month-old holstein calves that had received colostrum were inoculated endobronchially with bovine adenovirus 3 (bav-3). the gross and histologic lesions in these six infected calves were localized mainly in the right caudal lobe of the lung and were closely associated with the site of the deposition of the inoculum. the pneumonic lesions were severe necrotizing bronchitis, bronchiolitis, and alveolitis, accompanied by infiltration of inflammatory cells and proliferat ... | 2002 | 12243466 |
| gene therapy for prostate cancer delivered by ovine adenovirus and mediated by purine nucleoside phosphorylase and fludarabine in mouse models. | a gene-directed enzyme pro-drug therapy (gdept) based on purine nucleoside phosphorylase (pnp), that converts the prodrug, fludarabine to 2-fluoroadenine, has been described, but studies are limited compared with other gdepts. we investigated the in vitro and in vivo efficacies of pnp-gdept for treating androgen-independent (ai) prostate cancer. the pnp gene controlled by rous sarcoma virus (rsv) constitutive promoter was delivered using a recombinant ovine adenovirus vector (oadv220) that uses ... | 2002 | 12040457 |
| characterization of bovine adenovirus type 3 e1 proteins and isolation of e1-expressing cell lines. | like human adenovirus type 5 (hav5), bovine adenovirus type 3 (bav3) early region 1 (e1) consists of e1a and e1b transcriptional units. in order to characterize bav3 e1 proteins and to isolate a cell line of bovine origin that expresses bav3 e1, polyclonal antibodies specific to e1a, e1b-157r, and e1b-420r were raised in rabbits. bav3 e1a, e1b-157r, and e1b-420r were identified as 40, 17, and 47 kda proteins, and had a half-life of 45-60 min, and 4-6 and 4-6 h, respectively. it appeared that e1a ... | 2002 | 12033770 |
| development and characterization of bovine x human hybrid cell lines that efficiently support the replication of both wild-type bovine and human adenoviruses and those with e1 deleted. | the 293 cell line that was generated by transforming human embryonic kidney cells with human adenovirus type 5 (hav5) early region 1 (e1) sequences is an excellent host for generating and growing hav5 recombinants with e1 deleted, but it does not support the replication of bovine adenovirus type 3 (bav3). madin-darby bovine kidney (mdbk), an established bovine cell line, is an excellent host for growing and plaquing bav3. for the purpose of combining the unique characteristics of these two cell ... | 2002 | 12021321 |
| sequence analysis of porcine adenovirus serotype 5 fibre gene: evidence for recombination. | the nucleic acid and deduced amino acid sequence of the fibre gene of the hnf-61 strain of porcine adenovirus serotype 5 (padv-5) was determined and compared to that of the hnf-70 strain of the same serotype (nagy et al., j gen virol 82, 525-529, 2001) and also to adenovirus fibre genes from the genera mastadenovirus and atadenovirus. the putative hnf-61 and hnf-70 proteins were similar to each other, with 90% amino acid identity. conserved amino acid sequences described for mastadenovirus fibre ... | 2002 | 12018710 |
| preparation of ovine adenovirus vectors. | 2002 | 11987792 | |
| comparison of the deduced amino acid sequence of guinea pig adenovirus hexon protein with that of other mastadenoviruses. | we sought to isolate, clone, and determine the nucleic acid sequence of the guinea pig adenovirus (gpadv) hexon gene. from this, the amino acid sequence of the cloned portion was deduced and compared with a set of mastadenovirus hexons. | 2001 | 11922174 |
| cancer gene therapy: fringe or cutting edge? | direct targeting of cancer cells with gene therapy has the potential to treat cancer on the basis of its molecular characteristics. but although laboratory results have been extremely encouraging, many practical obstacles need to be overcome before gene therapy can fulfil its goals in the clinic. these issues are not trivial, but seem less formidable than the challenge of killing cancers selectively and rationally--a challenge that has been successfully addressed. | 2001 | 11905804 |
| mutational analysis of early region 4 of bovine adenovirus type 3. | the primary objective of characterizing bovine adenovirus type 3 (bav3) in greater detail is to develop it as a vector for gene therapy and vaccination of humans and animals. a series of bav3 early region 4 (e4) deletion-mutant viruses, containing deletions in individual e4 open reading frames (orf) or combinations of orfs, were generated by transfecting primary fetal bovine retinal cells with e4-modified genomic dna. each of these mutants was further analyzed for growth kinetics, viral dna accu ... | 2001 | 11883000 |
| molecular confirmation of an adenovirus in brushtail possums (trichosurus vulpecula). | partial genome characterisation of a non-cultivable marsupial adenovirus is described. adenovirus-like particles were found by electron microscopy (em) in the intestinal contents of brushtail possums (trichosurus vulpecula) in new zealand. using degenerate pcr primers complementary to the most conserved genome regions of adenoviruses, the complete nucleotide sequence of the penton base gene, and partial nucleotide sequences of the dna polymerase, hexon, and pvii genes were obtained. phylogenetic ... | 2002 | 11864751 |
| insulin gene transcription is mediated by interactions between the p300 coactivator and pdx-1, beta2, and e47. | pancreatic beta-cell-type-specific expression of the insulin gene requires both ubiquitous and cell-enriched activators, which are organized within the enhancer region into a network of protein-protein and protein-dna interactions to promote transcriptional synergy. protein-protein-mediated communication between dna-bound activators and the rna polymerase ii transcriptional machinery is inhibited by the adenovirus e1a protein as a result of e1a's binding to the p300 coactivator. e1a disrupts sig ... | 2002 | 11756538 |
| characterization of a cdna encoding the bovine coxsackie and adenovirus receptor. | non-human adenoviruses such as bovine adenovirus type 3 (bav-3) that do not replicate in human cells but can infect human cells in culture could provide an attractive alternative to human adenoviral vectors for gene therapy. in addition, a large-animal model for genetic diseases can be very useful for the assessment of the efficacy of adenovector-mediated gene delivery in man. recombinant human subgroup c adenovectors use the coxsackie and adenovirus receptor (car) to enter their target cells. t ... | 2001 | 11688979 |
| bovine adenovirus type 3 e1b(small) protein is essential for growth in bovine fibroblast cells. | in order to study the function of bovine adenovirus type 3 (bav-3) e1a and e1b(small) proteins, we constructed two mutants: (a) bav102a carries an in-frame deletion in the coding region for the e1a protein (nt 831-1080); (b) bav102b carries an insertion of triple stop codons in the e1b region (nt 1654, 178 bp downstream of the e1b(small) start codon), which stops the translation of the e1b(small) gene. bav102a virus could grow to the wild-type bav-3 titer in transformed cell line vido r2 (hav-5 ... | 2001 | 11601898 |
| characterization of a new adenovirus isolated from black-tailed deer in california. | an adenovirus associated with systemic and localized vascular damage was demonstrated by transmission electron microscopy and immunohistochemistry in a newly recognized epizootic hemorrhagic disease in california black-tailed deer. in this study, we describe the cultural, physicochemical and serological characteristics of a virus isolated from lung using neonatal white-tail deer lung and turbinate cell cultures. the virus had the cultural, morphological and physicochemical characteristics of mem ... | 2001 | 11504424 |
| gene transfer by viral vectors into blood vessels in a rat model of retinopathy of prematurity. | to test the feasibility of gene transfer into hyaloid blood vessels and into preretinal neovascularisation in a rat model of retinopathy of prematurity (rop), using different viral vectors. | 2001 | 11466260 |
| porcine adenoviruses: an update on genome analysis and vector development. | although porcine adenoviruses (padv) are present in the swine populations worldwide, they usually do not cause any disease, or the infection is only manifested in a mild diarrhoea or respiratory signs. the importance of adenoviruses, however, is constantly growing as there is a possibility of developing them into viral vector vaccines against more significant swine pathogens. a short summary of the well-established facts of porcine adenoviruses is given and recent developments of the genetic ana ... | 2000 | 11402666 |
| comparison of the genome of ovine adenovirus types 1 through 5 by restriction enzyme analysis and dna hybridisation. | the dna of the prototype strains of ovine adenovirus (oadv) 1 through 5 was analysed by restriction enzyme (re) digestion. the re patterns generated by hindiii and psti enzymes were characteristic of the examined strains. oadv-2 and 3 resembled each other the most, and their ecori and hindiii patterns seemed to be identical. considering the number of comigrating fragments, serotypes oadv-2, 3, 4 and 5 looked more closely related to each other than to oadv-1. this finding was strengthened by sout ... | 2000 | 11402664 |
| characterisation of the fiber gene and partial sequence of the early region 4 of bovine adenovirus 2 (short communication). | the full sequence of the fiber gene and partial sequence of the putative 17 kd protein gene of bovine adenovirus-2 (badv-2) were determined. the size of the fiber gene of badv-2 proved to be 561 amino acids, of which the amino acids 37 to 385 form a typical shaft domain of 22 repetitive motifs. on the complementary strand, a gene homologous to the 17 kd protein coded in the e4 region of several human adenoviruses was found. the sequence analysis seems to confirm the presence of an intron in the ... | 2001 | 11402654 |
| determination of bovine adenovirus-3 titer based on immunohistochemical detection of dna binding protein in infected cells. | dna sequence coding for a portion of dna binding protein (amino acids 3-58) of bovine adenovirus type-3 (bav-3) was cloned and expressed in escherichia coli as a fusion protein with schistosoma japonicum glutathione s-transferase. the fusion protein was affinity purified and used to immunize rabbits. immunoprecipitation and western blot analysis showed that the antiserum could specifically recognize a protein of 48 kda in bav-3-infected cells, which was produced both in early and late phases of ... | 2001 | 11337049 |
| four new inverted terminal repeat sequences from bovine adenoviruses reveal striking differences in the length and content of the itrs. | the inverted terminal repeat (itr) of the genome of four bovine adenovirus (badv) types have been sequenced, analysed and compared to the itrs of other adenoviruses. the length of itrs of the examined badvs ranged between 59 and 368 base pairs, thus the presently known longest adenovirus itr sequence is from badv-10. the conserved motifs and characteristic sequence elements of the itrs providing different binding sites for replicative proteins of viral and cellular origin seemed to be distribute ... | 2001 | 11324754 |
| alternate adenovirus type-pairs for a possible circumvention of host immune response to recombinant adenovirus vectors. | with the help of monoclonal antibodies the existence of at least 18 different earlier not known intertype (it) specific epitopes were demonstrated in different numbers and combinations on the hexons of different adenovirus serotypes. the it specific epitopes play an important role in the experimental gene therapy and in the recombinant adenovirus vaccination because of the harmful immune response of the recipient organisms directed against the many different epitopes of the adenovirus vector. fo ... | 2001 | 11233691 |
| the complete nucleotide sequence of porcine adenovirus serotype 5. | the complete nucleotide sequence of porcine adenovirus serotype 5 (padv-5) has been determined and the putative genomic map was constructed. the size of the genome was found to be 32621 nucleotides. twenty-eight putative orfs were identified by their homology to other adenovirus or other virus and eukaryotic genes. several special protein sequence motifs were identified by their homology to similar protein motifs. the putative promoter regions, polyadenylation and splice sites were predicted and ... | 2001 | 11172093 |
| construction and characterization of recombinant porcine adenovirus serotype 5 expressing the transmissible gastroenteritis virus spike gene. | five recombinant porcine adenoviruses of serotype 5 (padv-5) carrying the full-length or the 5' 2.2 kb half of the transmissible gastroenteritis virus (tgev) spike (s) gene were generated by homologous recombination in e. coli strain bj5183 cells and subsequent transfection of swine testicle cells. the foreign genes were inserted into the e3 region of padv-5. one recombinant virus had no deletion in the e3 region, whereas a 1.2 kb fragment was removed from the e3 region in the remainder of the r ... | 2001 | 11125171 |
| effect of adenovirus type 1 and influenza a virus on streptococcus pneumoniae nasopharyngeal colonization and otitis media in the chinchilla. | considerable evidence has implicated respiratory tract virus potentiation of bacterial adherence, colonization, and superinfection as a significant factor contributing to the pathogenesis of otitis media (om). influenza a and b viruses, adenovirus, and respiratory syncytial virus are the primary respiratory tract viruses associated with this disease. investigations have established a dramatic increase in the development of experimental om in chinchillas co-inoculated with influenza a virus and s ... | 2000 | 11089992 |
| optimization of bovine coronavirus hemagglutinin-estrase glycoprotein expression in e3 deleted bovine adenovirus-3. | adenoviral vectors expressing foreign genes have many desirable properties in applications such as vaccination. recently, we have generated replication-competent (e3 deleted) bovine adenovirus-3 (bav-3) recombinants expressing significant amounts of glycoprotein d (gd) of bovine herpesvirus-1 (a dna virus). however, attempts to express the rna virus genes using the same strategy were not successful. in an effort to optimize the expression, we have constructed several bav-3 recombinants carrying ... | 2000 | 11074126 |
| identification and sequence analysis of the core protein genes of bovine adenovirus 2. | the dna sequence of the genome of bovine adenovirus type 2 (badv-2) was determined between map units 42.5 and 50. by sequence analysis and homology search, the genes of five structural proteins were identified within this region: the penton base protein (iii; partial sequence), the major core protein precursor (pvii), the minor core protein (v), the mu core protein precursor (px) and the hexon associated protein precursor (pvi; partial sequence). the putative polypeptides were compared to their ... | 2000 | 11074122 |
| adenoviral gene transfer of nitric oxide synthase increases cerebral blood flow in rats. | depletion of nitric oxide may play a role in the development of vasospasm after aneurysmal subarachnoid hemorrhage. replenishment of nitric oxide might be a useful treatment for vasospasm. using rats, we performed intracisternal injections of replication-defective adenovirus containing the endothelial nitric oxide synthase (enos) gene and determined the localization of and effect on cerebral blood flow of transgene expression. | 2000 | 11063115 |
| blood clearance rates of adenovirus type 5 in mice. | persistence of adenovirus type 5 in blood has implications for the pathogenicity of the virus infection and for the use of this virus in oncolysis and gene therapy. in this study, the kinetics of adenovirus clearance from blood in mice has been evaluated. after a single inoculation of concentrated virus into the vena cava, virus half-life was less than 2 min. depletion of kupffer cells (kc) resulted in increased viraemia. after tail-vein injection, virus and latex beads co-localized within kc. a ... | 2000 | 11038370 |
| adenoviral vector-mediated rescue of the omp-null phenotype in vivo. | the use of gene deletion by homologous recombination to determine gene or protein function has wide application in vertebrate neurobiology. an ideal complement to gene deletion would be subsequent gene replacement to demonstrate re-acquisition of function. here we used an adenoviral vector to replace the olfactory marker protein (omp) gene in olfactory receptor neurons of adult omp-null mice and demonstrated the subsequent re-acquisition of function. our results show that short-term expression o ... | 2000 | 11036268 |
| ovine adenovirus vectors mediate efficient gene transfer to skeletal muscle. | ovine adenovirus (oav) vectors represent a promising tool for human gene therapy since these vectors overcome the problem of pre-existing immunity against human adenovirus vectors. in this report we investigated the in vivo characteristics of this novel vector system with respect to its potential for gene transfer into skeletal muscle. we found that moderate doses of an oav-derived vector expressing the human alpha1-antitrypsin gene (oavhaat) infected skeletal muscle in mice very efficiently res ... | 2000 | 11001369 |
| evaluation of the efficacy of 2',3'-dideoxycytidine against adenovirus infection in a mouse pneumonia model. | the antiviral activity of 2',3'-dideoxycytidine (ddc) has been investigated in a mouse pneumonia model. consolidation of lung, histopathological changes, dna synthesis as well as levels of tnfalpha were assayed. in this in vivo model, the oral administration of ddc twice daily over 4 days, displayed an inhibitory effect. the drug significantly reduced histopathologic responses. analysis indicated that under treatment pulmonary lesions were less severe than those of untreated controls. these data ... | 2000 | 10996395 |
| dna sequence of frog adenovirus. | the genome of frog adenovirus (fradv-1) was sequenced and found to be the smallest of all known adenovirus genomes. the sequence obtained was 26163 bp in size and contains a substantial direct repeat near the right terminus, implying that it was derived by recombination from a parental genome of only 25517 bp. the closest relative of fradv-1 proved to be turkey adenovirus 3, an avian adenovirus with no previously known near relative. sequence comparisons showed that the two viruses have equivale ... | 2000 | 10993931 |
| sequence analysis and deletion of porcine adenovirus serotype 5 e3 region. | a 3000 basepair (bp) region corresponding to the e3 region, the flanking pviii and part of the fiber protein genes, of the two prototype strains (hnf-61 and hnf-70) of porcine adenovirus serotype five (padv-5) was sequenced. a potential e3 promoter and poly-a signals were identified. the size of the e3 region was 2039 (strain hnf-61) and 2020 bp (strain hnf-70) the largest e3 so far reported among padvs. three open reading frames (orf2-4) were identified within the e3 region. based on the predic ... | 2000 | 10958982 |
| mouse adenovirus type 1 replication in vitro is resistant to interferon. | the effects of mouse interferon (ifn)-alpha/beta and recombinant ifn-gamma on mouse adenovirus type 1 (mav-1) replication were investigated in single-cycle infectious virus yield reduction assays on mouse l929 cells. viral yields at 3 days postinfection indicated that wt mav-1 and pme314, an early region 3 null mutant, were relatively insensitive to both ifn-alpha/beta and ifn-gamma, whereas early region 1a (e1a) mutants pme109 (null), dle105 (conserved region 1 deletion, cr1 delta), dle102 (cr2 ... | 2000 | 10936102 |
| serological evidence of adenovirus infection in cats. | the widespread presence of adenoviruses in various species makes it probable that infection and the carrier state also exist in cats. on the basis of these considerations, investigations were carried out to find antibodies against adenovirus in sera from different cat populations kept under different conditions. for the antibody detection, purified adenovirus was used in an indirect elisa. to produce positive serum, spf cats were immunized with a purified hexon preparation. altogether 632 field ... | 2000 | 10881688 |
| circumvention of vector-specific neutralizing antibody response by alternating use of human and non-human adenoviruses: implications in gene therapy. | to determine whether non-human adenovirus-specific antibodies are cross-neutralizing, rabbit and mouse anti-human adenovirus type 5 (had5), anti-bovine adenovirus type 3 (bad3), and anti-porcine adenovirus type 3 (pad3) sera were used in cross-virus neutralization assays. adenovirus neutralizing antibodies were found to be virus-specific, suggesting that virus neutralizing epitope differs significantly in had5, bad3, and pad3. to further investigate whether immunity to an had5-derived vector cou ... | 2000 | 10873758 |
| serologic detection of adenoviral hemorrhagic disease in black-tailed deer in california. | an enzyme-linked immunosorbent assay (elisa) and a serum neutralization (sn) test were developed to measure serum antibodies against the adenovirus causing hemorrhagic disease in free-ranging and captive experimentally-infected black-tailed deer (odocoilenus hemionus columbianus) in california (usa). there was a strong (rho = 0.874) and significant (p < 0.0001) correlation between elisa and sn titers, although the sn assay was more sensitive than the elisa. | 2000 | 10813622 |
| sequence analysis of porcine adenovirus type 3 e1 region, pix and piva2 genes, and two novel open reading frames. | the porcine adenovirus type 3 (pad3) genome between map units 0 and 13.7 was sequenced and compared with similar regions of other adenoviruses. this region consists of the left inverted terminal repeat sequences involved in dna packaging, the entire early region 1 (e1) and the protein ix (pix) transcription unit. the lower strand contains the c-terminal end of iva2 of the e2a transcriptional unit and two novel open reading frames (orfs). the e1 transcription unit consists of orfs for proteins ho ... | 2000 | 10773731 |
| an ovine adenovirus vector lacks transforming ability in cells that are transformed by ad5 e1a/b sequences. | adenoviruses of the mastadenovirus and aviadenovirus genera are able to transform certain cell types and induce tumor formation in susceptible animals. for the mastadenoviruses the e1a/b sequences are largely responsible for these properties but e4 sequences may also be involved. the transforming sequences of the aviadenoviruses, which lack e1a/b and e4 homologues, have not yet been fully identified. the recent proposal for a third genus of adenoviruses, which apparently lack an e1a homologue an ... | 2000 | 10772988 |
| dna sequencing and phylogenetic analysis of the protease gene of ovine adenovirus 3 suggest that adenoviruses of sheep belong to two different genera. | until now, the only published ovine adenovirus dna sequence was the complete genome of ovine adenovirus isolate 287 (oav287) which, compared to other mammalian adenoviruses, possesses strikingly unique genomic organisation and should properly be classified into a new adenovirus genus. the protease gene sequence of ovine adenovirus type 3 (oadv-3) was determined and analysed. the results of phylogenetic analysis of the 205 residue long protein demonstrated that oadv-3 belongs to the genus mastade ... | 2000 | 10653919 |
| normal development, wound healing, and adenovirus susceptibility in beta5-deficient mice. | integrins have been shown to play important roles in embryonic development, wound healing, metastasis, and other biological processes. alphavbeta5 is a receptor for rgd-containing extracellular matrix proteins that has been suggested to be important in cutaneous wound healing and adenovirus infection. to examine the in vivo function of this receptor, we have generated mice lacking beta5 expression, using homologous recombination in embryonic stem cells. mice homozygous for a null mutation of the ... | 2000 | 10629031 |
| adenovirus pneumonia in guineapigs: an experimental reproduction of the disease. | the recently described virus-induced pneumonia in guineapigs (naumann et al., 1981) was experimentally reproducible in newborn animals, though not in preadult animals. baby hamsters and newborn rats were also not susceptible to infection. 10 of 11 infected newborn guineapigs developed pathological changes identical with those found in spontaneous cases. the incubation period was from 5 to 10 days. the agent could not be cultivated in vitro, and therefore no applicable serological tests could be ... | 1984 | 10628789 |
| the distribution and kinetics of polyomavirus in lungs of intranasally infected newborn mice. | the primary cell types that sustain polyomavirus (py) replication following intranasal infection as well as the nature of the host cellular response to py were unknown. as this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. using immunohistochemistry and in situ hybridization, we determined the cell types in the lung that support py gene expression and replication following intranasal inoculation of newborn mi ... | 2000 | 10612660 |
| porcine adenovirus-3 as a helper-dependent expression vector. | porcine adenovirus has been proposed as a potential vector for generating novel and effective vaccines for pigs. as a prerequisite for the generation of helper-dependent porcine adenovirus-3 (pav-3) vectors, two e1-complementing porcine cell lines expressing e1 proteins of human adenovirus-5 (hav-5) were made. these cell lines could be efficiently transfected with dna and allowed the rescue and propagation of a pav-3 recombinant, pav201, containing a 0.597 kb e3 deletion and a 0.803 kb e1a delet ... | 1999 | 10580052 |
| novel human gene transfer vectors: evaluation of wild-type and recombinant animal adenoviruses in human-derived cells. | major disadvantages of human adenovirus (had) vectors in gene therapy include preexisting or induced immune responses, and possible coreplication of recombinant had in the presence of wild-type hads. these disadvantages may be overcome by using nonhuman, animal adenoviruses (aads). we evaluated four different aads for their potential use as viral vectors. the canine adenovirus type 2 (cav2) and bovine adenovirus type 3 (bav3) appeared to be suitable systems, as they infect human cells. cav2, but ... | 1999 | 10566887 |