Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| epidemiological study of the association between bovine gammaherpesvirus type 4 and reproductive disease in dairy cattle from northwestern spain. | bovine gammaherpesvirus 4 (bohv-4) has controversially been related with cattle reproductive disease. in the present study we analyze the relationship between exposure to bohv-4 and reproductive performance in dairy cattle from northwestern spain. a total of 2022 sera from 50 farms were examined to detect anti-bohv-4 antibodies. herd and individual reproductive records were collected to analyze association with exposure to bohv-4. in addition, 52 abortion cases were examined to detect bohv-4 dna ... | 2020 | 33120087 |
| prevalence of the main infectious causes of abortion in dairy cattle in algeria. | abortion in cattle is a major source of economic losses for the agriculture sector. it can be due to infectious or non-infectious factors. among infectious factors, parasites, bacteria, viruses, and fungi can be involved. the present work investigated the prevalence of the main infectious agents of abortion in algerian cattle. | 2017 | 29978092 |
| primary replication and invasion of the bovine gammaherpesvirus bohv-4 in the genital mucosae. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus that is widespread in cattle. ex vivo models with bovine genital tract mucosa explants were set up to study molecular/cellular bohv-4-host interactions. bovine posterior vagina, cervix and uterus body were collected from cows at two stages of the reproductive cycle for making mucosa explants. the bohv-4 replication kinetics and characteristics within the three different mucosae of animals in the follicular and luteal phase were assessed by viru ... | 2017 | 29183401 |
| induction of antihuman c-c chemokine receptor type 5 antibodies by a bovine herpesvirus type-4 based vector. | bovine herpesvirus 4 (bohv-4) is a promising vector for the delivery and intracellular expression of recombinant antigens and can thus be considered as a new prototype vaccine formulation system. an interesting, and actively pursued, antigen in the context of human immunodeficiency virus (hiv) infection prophylaxis (and therapy) is the c-c chemokine receptor type 5 (ccr5) co-receptor, whose blockage by specific antibodies has been shown to inhibit both viral entry and cell-to-cell transmission o ... | 2017 | 29118763 |
| coordinated role of toll-like receptor-3 and retinoic acid-inducible gene-i in the innate response of bovine endometrial cells to virus. | bovine herpesvirus-4 (bohv-4) and bovine viral diarrhea virus (bvdv) infect the uterus of cattle, often resulting in reduced fertility, or abortion of the fetus, respectively. here, exposure of primary bovine endometrial cells to bohv-4 or bvdv modulated the production of inflammatory mediators. viral pathogen-associated molecular patterns (pamps) are detected via pattern-recognition receptors (prrs). however, the relative contribution of specific prrs to innate immunity, during viral infection ... | 2017 | 28878771 |
| whole genome sequencing of a canadian bovine gammaherpesvirus 4 strain and the possible link between the viral infection and respiratory and reproductive clinical manifestations in dairy cattle. | bovine gammaherpesvirus 4 (bohv-4) is a herpesvirus widespread in cattle populations, and with no clear disease association. its genome contains a long unique coding region (lur) flanked by polyrepetitive dna and 79 open reading frames (orfs), with unique 17 orfs, named bo1 to bo17. in 2009, a bohv-4 strain was isolated (fmv09-1180503: bohv-4-fmv) from cattle with respiratory disease from quebec, canada, and its lur was sequenced. despite the overall high similarity, bohv-4-fmv had the most dive ... | 2017 | 28670580 |
| interleukin 8 haplotypes drive divergent responses in uterine endometrial cells and are associated with somatic cell score in holstein-friesian cattle. | interleukin 8 is a proinflammatory chemokine involved in neutrophil recruitment and activation in response to infection and also in the resolution of inflammation. our previous studies identified a number of genetic polymorphisms in the bovine il8 promoter region which segregate into two haplotypes, with balanced frequencies in the holstein-friesian (hf). we subsequently showed that these haplotypes confer divergent il8 activity both in vitro in mammary epithelial cells and in vivo in response t ... | 2017 | 28166928 |
| prevalence of bovine herpesvirus type 4 in aborting dairy cows. | bovine herpesvirus type 4 (bhv-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. in this study a retrospective pcr examination of 100 extracted dna samples from aborting cows was performed in order to determine: prevalence of bhv-4 in abortive cattle, whether coinfections bhv-4 with other abortifacient pathogens are present in the same samp ... | 2016 | 28092611 |
| bohv-4-based vector delivering ebola virus surface glycoprotein. | ebola virus (ebov) is a category a pathogen that is a member of filoviridae family that causes hemorrhagic fever in humans and non-human primates. unpredictable and devastating outbreaks of disease have recently occurred in africa and current immunoprophylaxis and therapies are limited. the main limitation of working with pathogens like ebov is the need for costly containment. to potentiate further and wider opportunity for ebov prophylactics and therapies development, innovative approaches are ... | 2016 | 27881138 |
| uterine infection with bovine herpesvirus type 4 in dairy cows. | diseases of the reproductive tract are a frequent problem in dairy herds. herpesviruses are uterine pathogens also involved in other clinical diseases; for example, bovine herpesvirus type 4 bohv-4 induces abortion, enteritis, metritis, pneumonia and vaginitis, but it can also be detected in healthy cows. the role of bohv-4 in the development of clinical endometritis (ce) or subclinical endometritis (se) has not clearly been described. therefore, the objective of this study was to describe the p ... | 2017 | 27859701 |
| virus-mediated metalloproteinase 1 induction revealed by transcriptome profiling of bovine herpesvirus 4-infected bovine endometrial stromal cells. | viral infections can cause genital tract disorders (including abortion) in cows, and bovine herpesvirus 4 (bohv-4) is often present in endometritis-affected animals. a major problem with cattle uterine viral infections in general, and bohv-4 in particular, is our limited understanding of the pathogenic role(s) that these infections play in the endometrium. a similar lack of knowledge holds for the molecular mechanisms utilized, and the host cell pathways affected, by bohv-4. to begin to fill the ... | 2016 | 27281703 |
| bovine herpesvirus 4-based vector delivering a hybrid rat/human her-2 oncoantigen efficiently protects mice from autochthonous her-2(+) mammary cancer. | the epidermal growth factor receptor 2 (her-2) oncogene is a major target for the immunotherapy of breast cancer. following up to the therapeutic success achieved with her-2-targeting monoclonal antibodies, immune-prophylactic approaches directed against her-2 have also been investigated taking into account, and trying to overcome, her-2 self-tolerance. perhaps due to safety (and efficacy) concerns, the least explored anti-her-2 active immunization strategy so far has been the one relying on vir ... | 2016 | 27141335 |
| the role of herpesviruses (bohv-1 and bohv-4) and pestiviruses (bvdv and bdv) in ruminant abortion cases in western turkey. | the economic impact of abortions in ruminant breeders is one of the biggest problems in livestock. of the infectious agents, viruses, especially herpesviruses and pestiviruses, are the most important causative agents of abortion in ruminants. in the present study, the role of herpesviruses (bovine herpesvirus-1 (bohv-1), bovine herpesvirus-4 (bohv-4)) and pestiviruses (bovine viral diarrhea virus (bvdv), border disease virus (bdv)) was investigated in cases of ruminant abortion between 2007 and ... | 2016 | 27038195 |
| seroprevalence study of the main causes of abortion in dairy cattle in morocco. | sera from 221 cattle were collected in 25 farms in morocco to investigate the evidence and circulation of some of the main bovine abortive agents in the dairy cattle farming, where abortions are often reported. all sera were examined for brucellosis, 176 for neosporosis, 88 for leptospirosis, and 42 for bovine viral diarrhoea (bvd/md), bovine herpesvirus 1 (bhv-1) (infectious bovine rhinotracheitis, ibr/ipv), and bovine herpesvirus 4 (bhv-4) infections (at least 1 sample per herd). abortions wer ... | 2017 | 27033527 |
| comparative study on the in vitro replication and genomic variability of argentinean field isolates of bovine herpesvirus type 4 (bohv-4). | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus, belonging to the rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. in argentina, analysis of bohv-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: genotype 1 comprises movar-like strains (european prototype), genotype 2 includes dn599-like strains (american prototype) ... | 2016 | 26995219 |
| laboratory findings suggesting an association between bohv-4 and bovine abortions in southern belgium. | abortions cause heavy economic losses for the bovine sector. the use of a standardized panel of analyses covering a large spectrum of pathogens responsible of abortion in cattle allowed demonstrating the direct involvement of at least one pathogen in 57% of analysed abortions in the southern part of belgium. this result suggests a margin of improvement in the diagnostic efficacy. in order to evaluate the interest to broaden the list of pathogens included in the panel of analyses, the implication ... | 2016 | 26752765 |
| co-infection with bovine herpesvirus 4 and histophilus somni significantly extends the service period in dairy cattle with purulent vaginal discharge. | the aim of the study was to investigate the effect of bovine herpesvirus 4 (bohv-4) and histophilus (h.) somni on fertility rate of cows in a hungarian holstein-friesian dairy herd with purulent vaginal discharge (pvd). non-pregnant cows (n = 188) with mature corpus luteum were treated with cloprostenol and 3 days later if they did not show oestrus, were examined by rectal palpation. animals showing pvd (n = 60/31.9%/) and 14 controls with normal vaginal discharge (score 0) were randomly selecte ... | 2016 | 26690881 |
| bovine herpesvirus 4 modulates its β-1,6-n-acetylglucosaminyltransferase activity through alternative splicing. | carbohydrates play major roles in host-virus interactions. it is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their profit different pathways of glycan synthesis. thus, the bo17 gene of bovine herpesvirus 4 (bohv-4) encodes a homologue of the cellular core 2 protein β-1,6-n-acetylglucosaminyltransferase-mucin type (c2gnt-m), which is a key player for the synthesis of complex o-glycans. surprisingly, we show in t ... | 2015 | 26656682 |
| search for the genome of bovine herpesvirus types 1, 4 and 5 in bovine semen. | bovine herpesvirus type 1 (bohv-1) causes respiratory and reproductive disorders in cattle. recently, bovine herpesvirus type 5 (bohv-5) and bovine herpesvirus type 4 (bohv-4) have been identified to be associated with genital disease. in this study, the presence of the genome of bohv-1, bohv-4 and bohv-5 in bovine semen of argentinean and international origin was analyzed by pcr assays. the most important finding of this study is the detection of the genome of bohv-1 and bohv-4 in semen of bull ... | 2013 | 26623325 |
| bovine herpesvirus 4 dna is not detected in free-ranging domestic cats from california, colorado or florida. | objectives several studies have reported that domestic cats can be naturally infected with bovine herpesvirus 4 (bhv4). cats experimentally inoculated with bhv4 developed clinical signs involving the urinary tract, leading to the hypothesis that natural infection with bhv4 may be associated with feline lower urinary tract diseases. however, the question of whether bhv4 infection is common in cats remains equivocal. in this study, we sought to determine whether bhv4 is a common natural infection ... | 2017 | 26450623 |
| bohv-4 immediate early 1 gene is a dispensable gene and its product is not a bone marrow stromal cell antigen 2 counteracting factor. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus whose genome was cloned as bacterial artificial chromosome (bac) and exploited as a gene delivery vector for vaccine purposes. although bohv-4 genome has been completely sequenced and its open reading frames (orfs) structurally defined in silico, most of them are not functionally characterized. in bohv-4 genome two major immediate early genes (ie) are present, ie1 and ie2. ie2 is an essential gene because its removal from the viral genome rende ... | 2015 | 26307352 |
| bovine herpesvirus 4 in parana state, brazil: case report, viral isolation, and molecular identification. | bovine herpesvirus 4 (bohv-4) is a member of gammaherpesvirinae sub-family and belongs to genus rhadinovirus . this virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. the goal of this work was to characterize a virus strain isolate from a cow's uterine outflow. from swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, ... | 2015 | 26221118 |
| molecular characterization of the first bovine herpesvirus 4 (bohv-4) strains isolated from in vitro bovine embryos production in argentina. | bovine herpesvirus 4 (bohv-4) is increasingly considered as responsible for various problems of the reproductive tract. the virus infects mainly blood mononuclear cells and displays specific tropism for vascular endothelia, reproductive and fetal tissues. epidemiological studies suggest its impact on reproductive performance, and its presence in various sites in the reproductive tract highlights its potential transmission in transfer-stage embryos. this work describes the biological and genetic ... | 2015 | 26177382 |
| epidemiology and genetic characterization of bvdv, bhv-1, bhv-4, bhv-5 and brucella spp. infections in cattle in turkey. | the aim of the study was to determine the epidemiological data of bovine viral diarrhea virus (bvdv), bovine herpesvirus-1 (bhv-1), bovine herpesvirus-4 (bhv-4), bovine herpesvirus-5 (bhv-5) and brucella-associated cattle that were previously reported to have abortion and infertility problems in ankara, corum, kirikkale and yozgat provinces, turkey. whole blood and sera samples were obtained from 656 cattle, and antibodies against brucella spp. were detected in 45 (6.86%) and 41 (6.25%) animals ... | 2015 | 26096964 |
| bohv-4-based vector single heterologous antigen delivery protects stat1(-/-) mice from monkeypoxvirus lethal challenge. | monkeypox virus (mpxv) is the etiological agent of human (mpx). it is an emerging orthopoxvirus zoonosis in the tropical rain forest of africa and is endemic in the congo-basin and sporadic in west africa; it remains a tropical neglected disease of persons in impoverished rural areas. interaction of the human population with wildlife increases human infection with mpx virus (mpxv), and infection from human to human is possible. smallpox vaccination provides good cross-protection against mpx; how ... | 2015 | 26086739 |
| potential pathogenetic role of bovine herpesvirus 4 in two dairy cows with dermatitis-pyrexia-hemorrhagic syndrome. | dermatitis, pyrexia, and hemorrhagic syndrome (dphs) is a rare bovine syndrome of unclear etiology. we describe two dphs cases, the first to occur in italy, with clinicopathological findings suggesting a potential pathogenetic role of bovine herpesvirus-4 (bohv-4). | 2015 | 26041898 |
| [bovine herpesvirus 4 (bohv-4): general aspects of the biology and status in argentina]. | bovine herpesvirus 4 (bohv-4) has been isolated from cattle with respiratory infections, vulvovaginitis, mastitis, abortions, endometritis and from apparently healthy animals throughout the world. although it has not yet been established as causal agent of a specific disease entity, it is primarily associated with reproductive disorders of cattle. this virus can infect a wide range of species, either in vivo or in vitro. two groups of prototype strains were originated from the first isolates: th ... | 2015 | 25962539 |
| use of tangential flow filtration for improving detection of viral adventitious agents in cell substrates. | in this study, we assessed the feasibility of tangential flow filtration (tff) for primary concentration of viral adventitious agents (aas) from large volumes of cell substrate-derived samples, such as cell-free chinese hamster ovary (cho) culture supernatants (500 ml) and cho cell lysates (50 ml), prior to virus detection in them by nucleic acid-based methods (i.e., qpcr and massively parallel sequencing (mps). the study was conducted using the samples spiked with four model dna viruses (bovine ... | 2015 | 25432087 |
| abortion in cattle due to infection with staphylococcus lugdunensis. | an aborted fetus of 7 months gestation, the associated placenta, and a single blood sample from the dam were submitted for diagnostic investigation to the diagnostic laboratory of the lombardy and emilia-romagna experimental zooprophylactic institute in parma, italy. the serum was negative for neospora caninum, coxiella burnetii, chlamydophila abortus, bovine herpesvirus 1 (bhv-1), bovine viral diarrhea virus (bvdv), brucella abortus, and brucella melitensis. fetal tissues and placental cotyledo ... | 2014 | 25292193 |
| interferon gamma-mediated bohv-4 replication restriction in bovine endometrial stromal cells is host ido1 gene expression independent and bohv-4 ie2 gene expression dependent. | in the present work the interaction between bovine herpesvirus 4 (bohv-4)-infected bovine endometrial stromal cells (bescs) and interferon gamma (ifng) was investigated. starting from the particular tropism of bohv-4 toward bescs, a pure population of these cells, free of cd45-positive cells, was prepared and proven to have a bona fide mesenchymal derivation as shown by vimentin-positive and cytokeratin-negative staining. bescs expressed functional ifng receptors (ifngr) 1 and 2 but not ifng lig ... | 2014 | 25273529 |
| detection of bovine herpesvirus type 4 antibodies and bovine lymphotropic herpesvirus in new zealand dairy cows. | to detect the presence of bovine herpesvirus (bohv) type 4 in new zealand dairy cows with clinical metritis. | 2014 | 24967608 |
| in vivo image analysis of bohv-4-based vector in mice. | due to its biological characteristics bovine herpesvirus 4 (bohv-4) has been considered as an appropriate gene delivery vector. its genomic clone, modified as a bacterial artificial chromosome (bac), is better genetically manipulable and can be used as an efficient gene delivery and vaccine vector. although a large amount of data have been accumulated in vitro on this specific aspect, the same cannot be asserted for the in vivo condition. therefore, here we investigated the fate of a recombinant ... | 2014 | 24752229 |
| detection of bovine herpesvirus 2 and bovine herpesvirus 4 dna in trigeminal ganglia of naturally infected cattle by polymerase chain reaction. | establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. in the case of bovine herpesvirus 2 (bohv-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (bohv-4) and ovine herpesvirus 2 (ovhv-2) latent virus targets on cells of the monocytic lineage. this study was conducted in quest of bohv-2, bohv-4 and ovhv-2 dna in two hundred trigeminal ganglia (tg) specimens, derived from one hundred clinically healthy c ... | 2014 | 24725448 |
| a gammaherpesvirus uses alternative splicing to regulate its tropism and its sensitivity to neutralization. | human gammaherpesviruses are associated with the development of lymphomas and epithelial malignancies. the heterogeneity of these tumors reflects the ability of these viruses to route infection to different cell types at various stages of their lifecycle. while the epstein barr virus uses gp42--human leukocyte antigen class ii interaction as a switch of cell tropism, the molecular mechanism that orientates tropism of rhadinoviruses is still poorly defined. here, we used bovine herpesvirus 4 (boh ... | 2013 | 24204281 |
| impact of bovine herpesvirus 4 (bohv-4) on reproduction. | bovine herpesvirus 4 (bohv-4) is increasingly considered as responsible for various reproductive troubles. this virus infects blood mononuclear cells and displays a specific tropism for vascular endothelia, mammary tissue, endometrium and foetal tissues. viral multiplication can be reactivated by corticosteroids or stress, both factors present at calving. bohv-4 has been isolated in a large variety of clinical cases, primarily metritis, vaginitis and mastitis, but also endometritis, abortion and ... | 2015 | 23998345 |
| presence of viral and bacterial organisms in milk and their association with somatic cell counts. | about 20 to 35% of milk samples from cows with intramammary infection or high somatic cell count (scc) are negative on bacteriological culture analysis. however, little is known about scc in milk of cows infected with viruses. in the first part of our study, we developed a real-time pcr assay for detection of bovine herpesvirus (bhv) 1, bhv2, and bhv4, and bovine viral diarrhea virus (bvdv) in composite quarter milk samples. a total of 1,479 lactating cows of 1,964 cows in the dairy herd were in ... | 2013 | 23972495 |
| comparison of nested pcr and qpcr for the detection and quantitation of bohv6 dna. | nested pcr and qpcr (quantitative pcr) tests based on glycoprotein b (gb) gene were designed for detecting bovine herpesvirus 6 (bohv6) in bovine whole blood samples and wild ruminant blood clots (deer and roe-deer). this virus, commonly known as blhv (bovine lymphotropic herpesvirus) belongs to the herpesviridae family, subfamily gammaherpesvirinae and macavirus genus. dna isolated from 92 dairy cow blood samples and 69 wild ruminant clots were examined for the presence of bohv6 using nested pc ... | 2013 | 23954301 |
| efficient heterologous antigen gene delivery and expression by a replication-attenuated bohv-4-based vaccine vector. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus belonging to the rhadinovirus genus and due to its biological characteristics has been proposed as a vaccine vector for veterinary vaccines. because viral vector-associated risk is a major concern for viral vector applications, attenuation is a desirable feature. therefore, efforts are directed toward the development of highly attenuated viral vectors. bohv-4 naturally exhibits limited pathogenicity and a further attenuation, in terms of replic ... | 2013 | 23830977 |
| bovine endometrial stromal cells support tumor necrosis factor alpha-induced bovine herpesvirus type 4 enhanced replication. | bovine uterine infections are the most important cause of economic losses in the cattle industry. although the etiology of uterine diseases is mainly ascribed to bacterial infection, they can also be associated with viral infection, such as bovine herpesvirus 4 (bohv-4), which is often a secondary agent following bacteria. besides microbial infection, many inflammatory molecules belonging to the innate immune response orchestrate the outcome of the infection. in the present study, the interactio ... | 2013 | 23515672 |
| bovine herpesvirus 4 glycoprotein b is indispensable for lytic replication and irreplaceable by vsvg. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus, belonging to rhadinovirus genus, with no clear association with disease. however, there is increasing evidence of its secondary pathogenic role in cases of post-partum metritis in cattle. bohv-4 open reading frame 8 (orf8) codifies for glycoprotein b (gb) that shows a heterodimeric structure, composed of two subunits and covalently linked by disulfide bonds and responsible for host cell adhesion through binding to heparan sulfates associated w ... | 2013 | 23302472 |
| clinical protection of goats against cphv-1 induced genital disease with a bohv-4-based vector expressing cphv-1 gd. | caprine herpesvirus type 1 (cphv-1) is an alphaherpesvirus causing genital disease leading to abortion in adult pregnant goats and a systemic disease with high morbility and mortality in kids. further, caprine herpesvirus 1 infection represents a valuable large animal model for human herpesvirus induced genital disease, exploitable for pathogenic studies, new vaccines and antiviral molecules testing. here, the bovine herpesvirus 4 (bohv-4) based vector derived from an apathogenic isolate of bohv ... | 2013 | 23300989 |
| bovine herpesvirus type 4 infection modulates autophagy in a permissive cell line. | bovine herpesvirus type 4 (bohv-4), like other herpesviruses, induces a series of alterations in the host cell that modify the intracellular environment in favor of viral replication, survival and spread. this research examined the impact of bohv-4 infection on autophagy in bohv-4 infected madin darby bovine kidney (mdbk) cells. protein extracts of bohv-4 infected and control mdbk cells were subjected to western blot. the concentrations of the autophagy and apoptosis-related proteins beclin 1, p ... | 2013 | 23297091 |
| identification and analysis of putative promoter motifs in bovine herpes virus. | the purpose of this study is to identify and analyse the putative promoter motifs in the bovine herpes virus which causes several diseases in cattle worldwide including bovine mastitis with large economic impact on dairy industry. bovine mastitis caused due to virus is often neglected as bacterial infections are held mainly responsible for the disease. therefore, in this in silico investigation with all the existing experimental data a total of 147 promoter were identified along with their seque ... | 2012 | 23275714 |
| proteomic characterization of bovine herpesvirus 4 extracellular virions. | gammaherpesviruses are important pathogens in human and animal populations. during early events of infection, these viruses manipulate preexisting host cell signaling pathways to allow successful infection. the different proteins that compose viral particles are therefore likely to have critical functions not only in viral structures and in entry into target cell but also in evasion of the host's antiviral response. in this study, we analyzed the protein composition of bovine herpesvirus 4 (bohv ... | 2012 | 22896609 |
| genomic analysis of bovine herpesvirus type 4 (bohv-4) from argentina: high genetic variability and novel phylogenetic groups. | bovine herpesvirus 4 (bohv-4) is a γ-herpesvirus that has been isolated both from apparently healthy animals and from cattle with a variety of clinical signs, including post-partum endometritis and abortion. bohv-4 causes either a persistent or a latent infection in cells of the monocyte/macrophage lineage. two groups of bovh-4 strains have been defined based on their restriction patterns: the movar-like strains (european prototype) and the dn 599-like strains (american prototype). the purpose o ... | 2012 | 22673320 |
| herpes simplex virus type 1 thymidine kinase-armed bovine herpesvirus type 4-based vector displays enhanced oncolytic properties in immunocompetent orthotopic syngenic mouse and rat glioma models. | gliomas are devastating tumors of the brain resistant to therapies. although some therapies can prolong the survival time among the affected persons, gliomas are not curable and new therapeutic approaches need to be investigated. oncolytic viruses seem to represent an interesting alternative, because anticancer agents and new viral agents have to be explored to identify the one with the best characteristics. bovine herpesvirus type 4 (bohv-4) is a gammaherpesvirus with a striking tropism and per ... | 2012 | 22228853 |
| bovine herpesvirus 4 glycoprotein l is non-essential for infectivity but triggers virion endocytosis during entry. | the core entry machinery of mammalian herpesviruses comprises glycoproteins b, h and l (gb, gh and gl). gh and gl form a heterodimer with a central role in viral membrane fusion. when archetypal alpha- or beta-herpesviruses lack gl, gh misfolds and progeny virions are non-infectious. however, the gl of the rhadinovirus murid herpesvirus 4 (muhv-4) is non-essential for infection. in order to define more generally what role gl plays in rhadinovirus infections, we disrupted its coding sequence in b ... | 2011 | 22205754 |
| antibody evasion by a gammaherpesvirus o-glycan shield. | all gammaherpesviruses encode a major glycoprotein homologous to the epstein-barr virus gp350. these glycoproteins are often involved in cell binding, and some provide neutralization targets. however, the capacity of gammaherpesviruses for long-term transmission from immune hosts implies that in vivo neutralization is incomplete. in this study, we used bovine herpesvirus 4 (bohv-4) to determine how its gp350 homolog--gp180--contributes to virus replication and neutralization. a lack of gp180 had ... | 2011 | 22114560 |
| sequencing of bovine herpesvirus 4 v.test strain reveals important genome features. | bovine herpesvirus 4 (bohv-4) is a useful model for the human pathogenic gammaherpesviruses epstein-barr virus and kaposi's sarcoma-associated herpesvirus. although genome manipulations of this virus have been greatly facilitated by the cloning of the bohv-4 v.test strain as a bacterial artificial chromosome (bac), the lack of a complete genome sequence for this strain limits its experimental use. | 2011 | 21846388 |
| symptomless intrauterine transmission of bovine herpesvirus 4 to bovine fetuses. | blood samples of 31 healthy calves and their dams taken immediately after calving before colostrum uptake, and at days 11, 23 and 8 weeks, spleens of seven stillborn calves were analysed in order to determine the source and time of bovine herpesvirus type 4 infection. all the calves were born as seronegatives, while all cattle were seropositives. viral dna were amplified by a nested pcr assay from 54.8% of peripheral blood leukocyte samples of newborn calves taken before colostrum uptake, and fr ... | 2011 | 21354299 |
| immunization of knock-out a/ß interferon receptor mice against lethal bluetongue infection with a bohv-4-based vector expressing btv-8 vp2 antigen. | new effective tools for vaccine strategies are necessary to limit the spread of bluetongue, an insect-transmitted viral disease of domestic and wild ruminants. in the present study, bohv-4-based vector cloned as a bacterial artificial chromosome (bac) was engineered to express the bluetongue virus (btv) immune-dominant glycoprotein vp2 provided of a heterologous signal peptide to its amino terminal and a trans-membrane domain to its carboxyl terminal (igk-vp2gdtm), to allow the vp2 expression ta ... | 2011 | 21320537 |
| swine adipose stromal cells loaded with recombinant bovine herpesvirus 4 virions expressing a foreign antigen induce potent humoral immune responses in pigs. | increasingly effective vaccination strategies are needed to counteract the high incidence of contagious diseases associated with intensive swine breeding. recombinant viral vaccines are a promising new avenue in this direction. key features of viral vectors suitable for immunoprophylaxis are safety, ease of manipulation and the ability to replicate in a variety of hosts. most of the above requirements are met by bovine herpesvirus 4 (bohv-4), a non-pathogenic dsdna virus capable of infecting a b ... | 2010 | 21115049 |
| the bovine herpesvirus 4 bo10 gene encodes a nonessential viral envelope protein that regulates viral tropism through both positive and negative effects. | all gammaherpesviruses encode a glycoprotein positionally homologous to the epstein-barr virus gp350 and the kaposi's sarcoma-associated herpesvirus (kshv) k8.1. in this study, we characterized the positional homologous glycoprotein of bovine herpesvirus 4 (bohv-4), encoded by the bo10 gene. we identified a 180-kda gene product, gp180, that was incorporated into the virion envelope. a bo10 deletion virus was viable but showed a growth deficit associated with reduced binding to epithelial cells. ... | 2010 | 21068242 |
| bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma. | the application of gene therapy for malignant gliomas is still under study and the use of specific vectors represents an important contribution. here, we investigated bovine herpesvirus 4 (bohv-4), which is non-pathogenic if injected into the rodent brain. we show that the vector can infect mouse, rat and human glioma cell lines and primary cultures obtained from human glioblastoma in vitro. bohv-4 was injected into a tumour grown in rat brain. although virus expression was scattered across the ... | 2010 | 21047419 |
| course and severity of postpartum metritis cases following antibiotic and pgf2α administration in postpartum metritis cows infected with bohv-4. | forty cows between day 1 and day 21 post-calving were examined for the presence of postpartum metritis in a dairy herd that had recently experienced an increase in metritis and that had previously tested positive against bovine herpes virus 4 (bohv-4) by various methods. antibodies against bohv-4 were detected in sera from 15 of 22 cows. for the virological study, uterine swab samples of 22 cows with metritis were used and tested for bohv-4 using polymerase chain reaction (pcr), virus isolation ... | 2010 | 21040510 |
| bovine herpesvirus 4 immediate early 2 (rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate u. | the orf50/rta gene has been shown to be an essential gene for many gammaherpesviruses. although the bohv-4 orf50/rta homolog, immediate early gene 2 (ie2), has been shown to activate several bohv-4 early and late promoters in cotransfection assays, there is no direct proof of its indispensability for progression of the virus to the lytic replication cycle in the context of the viral genome. in the present communication, replication defective bohv-4-v.test ie2 mutants were efficiently rescued, wi ... | 2010 | 21035279 |
| integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome. | persistent infection of macrophages with bovine herpesvirus 4 (bohv-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. mechanisms of maintenance of bohv-4 persistent infection are not understood. we previously generated in vitro models of bohv-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with bohv-4 carrying a drug-resistance marker, and demonstrated circular ... | 2010 | 20854697 |
| the chemokine il8 is up-regulated in bovine endometrial stromal cells by the bohv-4 ie2 gene product, orf50/rta: a step ahead toward a mechanism for bohv-4 induced endometritis. | postpartum infections of the endometrium and metritis are common causes of delayed conception and infertility in cattle. these infections are characterized by inflammation of the endometrium and secretion of the chemokine interleukin 8 (il8), which attracts granulocytes to the endometrium. bovine herpesvirus 4 (bohv-4) is tropic for the endometrium and the only virus consistently associated with postpartum metritis. the bohv-4 immediate early 2 (ie2) gene is the first viral gene transcribed by h ... | 2010 | 20720165 |
| the possible role of bovine herpesvirus type-4 infection in cow infertility. | experimental and field studies have proven that the bovine herpesvirus type-4 (bhv-4) infection leads to various reproductive system problems. in this study, the role of bhv-4 infection in repeat breeding was investigated serologically. eighty-four samples were obtained from repeat-breeding diagnosed cows in two organized dairy herds; an equal number of healthy cows were sampled from the same farms. the rest of the samples (105) were obtained from reproductively normal cows that were breeding in ... | 2010 | 20597886 |
| bovine herpesvirus 4 orf73 is dispensable for virus growth in vitro, but is essential for virus persistence in vivo. | orf73 orthologues encoded by different rhadinoviruses have been studied extensively. these studies revealed that the orf73 expression product (porf73) is a multifunctional protein essential for latency that enables episome tethering to mitotic chromosomes and modulates cellular pathways implicated in growth and survival of latently infected cells. comparison of porf73 orthologues encoded by rhadinoviruses reveals important variations in amino acid sequence length and composition. bovine herpesvi ... | 2010 | 20592111 |
| cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine vector development. | in a previous study, an apathogenic strain of bovine herpesvirus 4 (bohv-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (ge2/gd) as a secreted form was described. recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (bvdv) ge2 and bohv-1 gd. however, neutralizing antibodies were produced only against bvdv, not against bohv-1. in the present work a recombinant bohv-4 expressing a membrane-linked form of ge2/gd chimeric pept ... | 2009 | 19793901 |
| isolation and characterization of bovine herpesvirus 4 (bohv-4) from a cow affected by post partum metritis and cloning of the genome as a bacterial artificial chromosome. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus with a worldwide distribution in cattle and is often isolated from the uterus of animals with postpartum metritis or pelvic inflammatory disease. virus strain adaptation to an organ, tissue or cell type is an important issue for the pathogenesis of disease. to explore the mechanistic role of viral strain variation for uterine disease, the present study aimed to develop a tool enabling precise genetic discrimination between strains of bohv-4 and ... | 2009 | 19691825 |
| anchoring tick salivary anti-complement proteins irac i and irac ii to membrane increases their immunogenicity. | tick salivary proteins are promising targets for the development of anti-tick vaccines. recently, we described two paralogous anti-complement proteins, called ixodes ricinus anti-complement (irac) proteins i and ii, that are co-expressed in tick i. ricinus salivary glands. however, our previous attempts to immunize rabbits against irac via infection with recombinant bovine herpesvirus 4 (bohv-4) vectors invariably failed although both recombinants expressed high levels of functional irac protein ... | 2009 | 19531344 |
| defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. | uterine microbial disease affects half of all dairy cattle after parturition, causing infertility by disrupting uterine and ovarian function. infection with escherichia coli, arcanobacterium pyogenes, and bovine herpesvirus 4 causes endometrial tissue damage. toll-like receptors on endometrial cells detect pathogen-associated molecules such as bacterial dna, lipids, and lipopolysaccharide (lps), leading to secretion of cytokines, chemokines, and antimicrobial peptides. chemokines attract neutrop ... | 2009 | 19439727 |
| double immunization strategy with a bohv-4-vectorialized secreted chimeric peptide bvdv-e2/bohv-1-gd. | a bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. so cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein e2 ectodomain with the bovine herpesvirus 1 glycoprotein d ectodomain. recombinant virus infected cells robustly expressed and secreted the chimeric peptide i ... | 2008 | 18812200 |
| dry-heat treatment process for enhancing viral safety of an antihemophilic factor viii concentrate prepared from human plasma. | viral safety is a prerequisite for manufacturing clinical antihemophilic factor viii concentrates from human plasma. with particular regard to the hepatitis a virus (hav), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor viii concentrate. the loss of factor viii activity during dry-heat treatment was of about 5%. no substantial changes were observed in the physi ... | 2008 | 18633304 |
| bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: a new insight into bacterial and viral interaction for uterine disease. | experimental infection with the gamma-herpesvirus bovine herpesvirus 4 (bohv-4) rarely establishes disease, yet bohv-4 is commonly associated with uterine disease in cattle. uterine disease involves co-infection with bacteria such as escherichia coli, which stimulate the production of prostaglandin e(2) (pge(2)) by endometrial cells. bohv-4 replication depends on immediate early 2 (ie2) gene transactivation and, in the present study, pge(2), e. coli or its lipopolysaccharide upregulated the ie2 ... | 2008 | 18577555 |
| herpes simplex virus type 1 icp27 regulates expression of a variant, secreted form of glycoprotein c by an intron retention mechanism. | we previously showed that herpes simplex virus type 1 (hsv-1) immediate-early (ie) protein icp27 can posttranscriptionally stimulate mrna accumulation from a transfected viral late gene encoding glycoprotein c (gc) (k. d. perkins, j. gregonis, s. borge, and s. a. rice, j. virol. 77:9872-9884, 2003). we began this study by asking whether icp27 homologs from other herpesviruses can also mediate this activity. although the homologs from varicella-zoster virus (vzv) and human cytomegalovirus (hcmv) ... | 2008 | 18495765 |
| uterine diseases in cattle after parturition. | bacterial contamination of the uterine lumen is common in cattle after parturition, often leading to infection and uterine disease. clinical disease can be diagnosed and scored by examination of the vaginal mucus, which reflects the presence of pathogenic bacteria such as escherichia coli and arcanobacterium pyogenes. viruses may also cause uterine disease and bovine herpesvirus 4 (bohv-4) is tropic for endometrial cells, causing a rapid cytopathic effect. the elimination of pathogens by the inn ... | 2008 | 18329302 |
| transduction of the rat brain by bovine herpesvirus 4. | bovine herpesvirus 4 (bohv-4) is a gamma-herpesvirus with no clear disease association. a recombinant bohv-4 (bohv-4egfp delta tk) expressing green fluorescent protein (egfp), was successfully used to infect f98 rat glioma cells. bohv-4egfp delta tk was injected into the lateral ventricle of the rat brain. histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. clinical scores, evaluated for 90 days, indicated that the v ... | 2008 | 18267037 |
| assessment of bovine herpesvirus 4 based vector in chicken. | the biological characteristics of bohv-4 make it a good candidate as a gene delivery vector for vaccination purposes. these characteristics include little or no pathogenicity, unlikely oncogenicity, the ability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, such as sheep, goats, swine, cats, dogs, rabbits, mink, horses, turkeys, ferrets, monkeys, hamsters, rats, mice, and chickens. in this report, the feasibility ... | 2008 | 18243344 |
| natural antibody--complement dependent neutralization of bovine herpesvirus 4 by human serum. | in contrast to most gammaherpesviruses, bovine herpesvirus 4 (bohv-4) has a broad range of host species both in vitro and in vivo. several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to bohv-4. these observations led to the hypothesis that cross-species transmission of bohv-4 could lead to human infections. in the present study, we investigate the sensitivity of bohv-4 to neutralization by naïve human sera in order to determine if humans exhibit inna ... | 2007 | 18024120 |
| establishment of a bovine herpesvirus 4 based vector expressing a secreted form of the bovine viral diarrhoea virus structural glycoprotein e2 for immunization purposes. | the biological characteristics of bohv-4 make it a good candidate as a gene delivery vector for vaccination purposes. these characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. | 2007 | 17945009 |
| detection of bovine herpesvirus 4 dna in aborted bovine fetuses. | the presence of bovine herpesvirus 4 (bohv-4) was investigated by several methods in 24 aborted bovine fetuses. polymerase chain reaction (pcr) and in situ dna hybridization proved the presence of bohv-4 dna in 7 (29%) of the fetuses. the bohv-4 genome was detected in the cytoplasm of splenic lymphocytes and monocytes, and sometimes in renal tubular epithelial cells or hepatic kupffer cells, in all 7 pcr-positive fetuses. however, bohv-4-specific monoclonal antibody failed to detect viral antige ... | 2007 | 17695599 |
| bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function. | bovine postpartum uterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. although the gamma-herpesvirus bovine herpesvirus 4 (bohv-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses in endometrial pathology and the mechanisms of viral infection of uterine cells are often ignored. the objectives of the present study were to explore the interaction, tropism and outcomes of bohv-4 challenge of endometrial ... | 2007 | 17641100 |
| an investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis. | uteri from 31 infertile cattle were examined for the presence of bovine herpesvirus 4 (bohv-4) by nested polymerase chain reaction (pcr). samples were also tested for bacteria, including chlamydiae and mycoplasma bovis. bohv-4 was detected by pcr in 27/31 (87.1%) samples, but the presence and amount of viral dna was not correlated with histological and bacteriological findings. arcanobacterium pyogenes, histophilus somni and pasteurella multocida were isolated from five cows with endometritis. c ... | 2008 | 17572123 |
| bovine lymphotropic herpesvirus and non-responsive post-partum metritis in dairy herds in the uk. | bovine lymphotropic herpesvirus (blhv) was detected for the first time in the uk in december 2005 in a dairy herd suffering from chronic, non-responsive post-partum metritis (nppm). a small-scale investigation was undertaken in order to determine whether this was an isolated case. samples of vaginal exudates or vaginal swabs were collected from cows in 13 uk dairy herds with a history of post-partum metritis that had not responded to standard treatment regimes for this condition. cows in 9/13 he ... | 2008 | 17408995 |
| oncolytic viruses in cancer therapy. | oncolytic virotherapy is a promising form of gene therapy for cancer, employing nature's own agents to find and destroy malignant cells. the purpose of this review is to provide an introduction to this very topical field of research and to point out some of the current observations, insights and ideas circulating in the literature. we have strived to acknowledge as many different oncolytic viruses as possible to give a broader picture of targeting cancer using viruses. some of the newest additio ... | 2007 | 17383089 |
| first serbian isolates of bovine herpesvirus 4 (bohv-4) from a herd with a history of postpartum metritis. | two farms in the belgrade area have experienced serious problems with postpartal metritis. serological examination of bohv-4 infection was done using elisa test and vaginal swabs were used for virus isolation. average seroprevalence of bohv-4 in these farms was 84.37%. bohv-4 isolation was successful from three vaginal swabs on the mdbk cell line. rising values of bohv-4 antibodies were recorded in nine of ten cows with clinical signs of postpartal metritis. pcr and restriction analysis were use ... | 2007 | 17319601 |
| generation of a transposon insertion mutant library for bovine herpesvirus 4 cloned as a bacterial artificial chromosome by in vitro mua based dna transposition system. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus with no clear disease association. although the bohv-4 genome has been sequenced, the function of the majority of putative genes is elusive. several features make bohv-4 attractive as a backbone for use as a viral expression vector and/or as a model to study gamma herpesvirus biology and determining which genes are essential for its replication is a very important task. starting from bohv-4 genome cloned as infectious bacterial artificial chrom ... | 2007 | 17182112 |
| detection of bovine herpesvirus 4 in aborted bovine placentas. | diagnostic studies on aborted placentas of cattle usually do not determine any reason for abortions. in this paper, five bovine herpesviruses (bohvs) and some bacteriological agents were investigated by several methods in 33 aborted bovine placentas. inclusion bodies, pcr and in situ dna hybridization proved the presence of bohv-4 dna in six (18.18%) tested placentas. positive dna hybridization signal localized bohv-4 dna to placental epithelial cells macrophages and lymphocytes. by destroying e ... | 2006 | 16950594 |
| recombinant bovine herpesvirus 4 (bohv-4) expressing glycoprotein d of bohv-1 is immunogenic and elicits serum-neutralizing antibodies against bohv-1 in a rabbit model. | several biological characteristics of bovine herpesvirus 4 (bohv-4) make it a good candidate as a gene delivery vector for vaccination purposes. these characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types coming from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. starting from bohv-4 c ... | 2006 | 16928886 |
| trypsin treatment of bovine embryos after in vitro exposure to infectious bovine rhinotracheitis virus or bovine herpesvirus-4. | the objectives of this study were to evaluate the efficacy of trypsin treatment for the removal/inactivation of infectious bovine rhinotracheitis virus (ibrv) adhering to zona pellucida-intact (zp-i) bovine embryos and to determine if bovine herpesvirus-4 (bhv-4) adheres to zp-i bovine embryos. when adherence of bhv-4 was demonstrated, an additional objective was to determine whether trypsin treatment removes or inactivates this virus. a total of 139 zp-i embryos was collected from superovulated ... | 1990 | 16726850 |
| bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo. | gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. bovine herpesvirus 4 (bohv-4) based vector capability to transduce rat liver cells in vitro and in vivo was assessed. for the in vitro study, a buffalo rat liver cell line was successfully transduced by bohv-4 and although did not show toxicity, the immediate early two viral gene was transcribed and cells harboring the intact viral genome could be pharmaco ... | 2006 | 16712963 |
| evolution of bovine herpesvirus 4: recombination and transmission between african buffalo and cattle. | bovine herpesvirus 4 (bohv-4) has been isolated from cattle throughout the world, but virological and serological studies have suggested that the african buffalo is also a natural host for this virus. it has previously been found that the bo17 gene of bohv-4 was acquired from an ancestor of the african buffalo, probably around 1.5 million years ago. analysis of the variation of the bo17 gene sequence among bohv-4 strains suggested a relatively ancient transmission of bohv-4 from the buffalo to t ... | 2006 | 16690915 |
| characterization of bovine herpesvirus type 4 isolated from cattle with mastitis and subclinical infection by the virus among cattle. | an outbreak of contagious mastitis occurred among cattle on a farm, and bovine herpesviruses were isolated from the affected mammary tissues, scabs and abscess discharge of the cattle. a bovine herpesvirus type 4 (bohv-4)-specific fragment was amplified from the isolates by polymerase chain reaction (pcr). restriction endonuclease analyses demonstrated that the isolates were related to movar-like european type bohv-4. to determine the ratio of bohv-4 subclinical infection in the cattle, a genomi ... | 2006 | 16520546 |
| outcome of bovine herpesvirus 4 infection following direct viral injection in the lateral ventricle of the mouse brain. | a recombinant bovine herpesvirus 4 (bohv-4egfpdeltatk), obtained by the insertion of an egfp gene into the tk locus of dn 599 bohv-4 strain, was injected into the lateral ventricle of the brain of mice and a clinical score was evaluated for 90 days. although bohv-4 was not neuro-pathogenic, bohv-4egfpdeltatk transduction capability was analyzed. egfp expression was localized in close proximity to the border of the ventricles and egfp-positive cells were found to co-localize with ependymal cells. ... | 2006 | 16503181 |
| pathogenesis of gammaherpesvirus infections. | gammaherpesviruses are members of an emerging subfamily among the herpesviridae. two genera are discriminated: (i) lymphocryptovirus, including its type species epstein-barr virus (ebv), and (ii) rhadinovirus, including viruses of interest for medicine, veterinary medicine, and biomedical research, i.e. alcelaphine herpesvirus 1, bovine herpesvirus 4, equine herpesvirus 2, human herpesvirus 8, mouse herpesvirus 68, and ovine herpesvirus 2 (ovhv-2). the perception that these viruses have a narrow ... | 2006 | 16332416 |
| virological and serological evidence of bovine herpesvirus type 4 in cattle in northern ireland. | bovine herpesvirus type 4 (bhv-4), a member of the genus rhadinovirus, subfamily gammaherpesvirinae, within the family herpesviridae, was isolated in fetal bovine lung cells from samples of vaginal discharge taken from a dairy herd in which approximately 50 per cent of the cattle developed metritis after calving. the identity of the isolate was confirmed by immunofluorescent staining with a bhv-4-specific monoclonal antibody and partial sequencing of a portion of the glycoprotein b gene. serolog ... | 2005 | 16258133 |
| pcr detection of bovine herpesviruses from nonbovine ruminants in hungary. | polymerase chain reaction (pcr) was used to test six different nonbovine ruminant species for five bovine herpesviruses including infectious bovine rhinotracheitis virus (bohv-1), bovine herpes mammillitis virus (bohv-2), movar-type herpesvirus (bohv-4), bovine herpesvirus type 5 (bohv-5), and alcelaphine herpesvirus 1 (alhv-1). species tested included 56 roe deer (capreolus capreolus), 66 red deer (cervus elaphus), 20 fallow deer (dama dama), 16 mouflon (ovis musimon), 34 domestic sheep, and 44 ... | 2005 | 16244057 |
| bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo. | the idea of using oncolytic viruses for the treatment of cancers was proposed a century ago. during the last two decades, viruses able to replicate specifically in cancer cells and to induce their lysis were identified and were genetically modified to improve their viro-oncolytic properties. more recently, a new approach consisting of inducing selective apoptosis in cancer cells through viral infection has been proposed; this approach has been called viro-oncoapoptosis. in the present study, we ... | 2005 | 16230410 |
| a novel subgroup of rhadinoviruses in ruminants. | in the course of investigating the malignant catarrhal fever (mcf) subgroup of rhadinoviruses, seven novel rhadinoviruses were identified in a variety of ruminants, including domestic sheep, bighorn sheep, bison, black-tailed deer, mule deer, fallow deer, elk and addax. based on the dna polymerase gene sequences, these newly recognized viruses clustered into a second distinct subgroup in ruminants with three members identified previously in cattle, goats and oryx. phylogenetic analysis revealed ... | 2005 | 16227223 |
| antibodies against bovine herpesvirus 4 are highly prevalent in wild african buffaloes throughout eastern and southern africa. | bovine herpesvirus 4 (bohv-4) has been isolated from cattle throughout the world. interestingly, a survey of wild african buffaloes mainly from the maasai mara game reserve in kenya revealed that 94% of the animals tested had anti-bohv-4 antibodies [rossiter, p.b., gumm, i.d., stagg, d.a., conrad, p.a., mukolwe, s., davies, f.g., white, h., 1989. isolation of bovine herpesvirus-3 from african buffaloes (syncerus caffer). res. vet. sci. 46, 337-343]. these authors also proposed that the serologic ... | 2005 | 16153785 |
| short communication: pasteurization of milk abolishes bovine herpesvirus 4 infectivity. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus highly prevalent in the cattle population that has been isolated from the milk and the serum of healthy infected cows. several studies reported the sensitivity and the permissiveness of some human cells to bohv-4 infection. moreover, our recent study demonstrated that some human cells sensitive but not permissive to bohv-4 support a persistent infection protecting them from tumor necrosis factor-alpha-induced apoptosis. together, these observat ... | 2005 | 16107396 |
| potential secondary pathogenic role for bovine herpesvirus 4. | bovine herpesvirus 4 (bohv-4) is a gammaherpesvirus with no clear disease association. previous studies have demonstrated that macrophages can harbor persistent bohv-4. we found that the addition of prostaglandin e2 (pge2) to bovine macrophage cells persistently infected with bohv-4 increases viral replication. because opportunistic infection can increase pge2 production, we propose a link between opportunistic infection, pge2 production, and bohv-4 replication. | 2005 | 16000468 |
| detection of bovine herpesvirus 4 in cd11b+ leukocytes of experimentally infected rabbits. | the presence and numbers of bovine herpesvirus 4 (bohv-4) infected cd11b+ leukocytes were investigated during experimental infections of new zealand white rabbits by fluorescence activated cell sorter (facs) analysis. peripheral blood leukocytes (pbl) were collected every second day, and the cells were stained with phycoerythrin-labelled cd11b-specific mouse monoclonal antibody and fluorescein-conjugated bovine herpesvirus 4-specific mouse monoclonal antibody. the numbers of double-stained cells ... | 2005 | 15959985 |
| bovine herpesvirus 4-associated postpartum metritis in a spanish dairy herd. | in more than 10 spanish dairy cows, a bovine herpesvirus 4 (bhv4) associated postpartum metritis was confirmed by virus isolation, bhv4-glycoprotein b (gb) pcr and/or serology. in this study, 12 cows with, and, at the time of sampling, 3 cows without clinical signs of acute postpartum metritis from one large dairy herd in spain were examined for bacterial and viral infections. blood, placenta/caruncles and uterine contents were collected between day 1 and day 20 post-calving, and examined for th ... | 2006 | 15946713 |
| exploiting persistent infection for selection of bovine herpesvirus 4 recombinants. | bovine herpesvirus 4 (bohv-4) is a gamma-herpesvirus with no clear disease association, and due to its biological characteristics, has been suggested as a gene delivery vector. it was demonstrated previously that recombinant bohv-4 carrying a neomycin-resistance gene was able to infect a human rhabdomyosarcoma cell line (rd-4), resulting in no detectable cytopathic effect (cpe) and allowing selection of g418-resistant persistently-infected cells containing circular episomal viral dna [donofrio, ... | 2005 | 15885813 |
| susceptibility of bovine mesenchymal stem cells to bovine herpesvirus 4. | bovine herpesvirus 4 (bohv-4) is a gamma herpesvirus with no clear disease association. previous studies have demonstrated that macrophages can harbour persistent bohv-4. since mesenchymal stem cells in bone marrow regulate the differentiation and proliferation of adjacent haematopoietic precursors, such as macrophages, the interaction between bohv-4 and mesenchymal stem cells was investigated. primary bovine mesenchymal stem cells were highly permissive to support full replication of bohv-4. th ... | 2005 | 15869810 |
| development of bovine herpesvirus 4 as an expression vector using bacterial artificial chromosome cloning. | several features make bovine herpesvirus 4 (bohv-4) attractive as a backbone for use as a viral expression vector and/or as a model to study gammaherpesvirus biology. however, these developments have been impeded by the difficulty in manipulating its large genome using classical homologous recombination in eukaryotic cells. in the present study, the feasibility of exploiting bacterial artificial chromosome (bac) cloning and prokaryotic recombination technology for production of bohv-4 recombinan ... | 2005 | 15784885 |