Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| transposable element misregulation is linked to the divergence between parental pirna pathways in drosophila hybrids. | interspecific hybridization is a genomic stress condition that leads to the activation of transposable elements (tes) in both animals and plants. in hybrids between drosophila buzzatii and drosophila koepferae, mobilization of at least 28 tes has been described. however, the molecular mechanisms underlying this te release remain poorly understood. to give insight on the causes of this te activation, we performed a te transcriptomic analysis in ovaries (notorious for playing a major role in te si ... | 2017 | 28854624 |
| a biophysical interpretation of temperature-dependent body size in drosophila aldrichi and d. buzzatii. | the temperature-size rule, the observation that most ectotherms grow faster but reach smaller size at higher temperatures, has defied a general explanation. here, the temperature-size rule in drosophila aldrichi and drosophila buzzatii is investigated, using data on development rate and adult dry weight at nine temperatures. in both species the linear regression of dry weight on temperature is negative. the data are used to infer the potential for a description of temperature dependent size by b ... | 2010 | 28799917 |
| mating pattern and fitness-component analysis associated with inversion polymorphism in a natural population of drosophila buzzatii. | direct studies of mating success or mating pattern associated with mendelian factors rarely have been carried out in nature. from the samples taken for the standard analyses of selection components, it is not usually possible to obtain the mating table, and only directional selection for male mating success can be detected. both processes, mating pattern and differential mating probability, together with other fitness components, have been investigated for the inversion polymorphism of a natural ... | 1994 | 28568257 |
| evolutionary history of drosophila buzzatii. ii. how much has chromosomal polymorphism changed in colonization? | 1982 | 28568228 | |
| antagonistic pleiotropic effect of second-chromosome inversions on body size and early life-history traits in drosophila buzzatii. | a simple way to think of evolutionary trade-offs is to suppose genetic effects of opposed direction that give rise to antagonistic pleiotropy. maintenance of additive genetic variability for fitness related characters, in association with negative correlations between these characters, may result. in the cactophilic species drosophila buzzatii, there is evidence that second-chromosome polymorphic inversions affect size-related traits. because a trade-off between body size and larval developmenta ... | 1998 | 28568158 |
| selection for heat-shock resistance in larval and in adult drosophila buzzatii: comparing direct and indirect responses. | direct and correlated responses in selection for heat-shock resistance in adult and in larval drosophila buzzatii were studied. two lines were artificially selected for higher survival to heat stress as adults, and two other lines were reared under a fluctuating thermal environment as larvae, 35°c for 6 h and 25°c for 18 h, to "naturally" select for higher resistance as larvae. the latter two lines were duplicated after nine generations to yield additional lines to be "naturally" selected as lar ... | 1996 | 28565653 |
| apparent directional selection of body size in drosophila buzzatii: larval crowding and male mating success. | 1996 | 28565651 | |
| inversion length and breakpoint distribution in the drosophila buzzatii species complex: is inversion length a selected trait? | length and position of breakpoints are characteristics of inversions that can be precisely determined on the polytene chromosomes of drosophila species, and they provide crucial information about the processes that govern the origin and evolution of inversions. eighty-six paracentric inversions described in the drosophila buzzatii species complex and 18 inversions induced by introgressive hybridization in d. buzzatii were analyzed. in contrast to previous studies, inversion length and breakpoint ... | 1997 | 28565492 |
| inversion polymorphism, longevity, and body size in a natural population of drosophila buzzatii. | in this study we present the results of an analysis of differential longevity associated with drosophila buzzatii second chromosome inversion karyotypes based on the assessment of more than 1000 individuals collected in a natural population. comparisons of inversion frequencies between emerged and bait-collected flies showed not only that inversion arrangements were associated with differential longevity, but also that selection was sex specific. because each individual fly was scored for thorax ... | 1999 | 28565408 |
| evolutionary history of drosophila buzzatii. i. natural chromosomal polymorphism in colonized populations of the old world. | 1981 | 28563456 | |
| isozyme variation in natural populations of drosophila buzzatii. | 1976 | 28563041 | |
| the evolutionary history of drosophila buzzatii. viii. evidence for endocyclic selection acting on the inversion polymorphism in a natural population. | the pattern of selection acting in nature on the chromosomal polymorphism of the cactophilic species drosophila buzzatii was investigated by comparing inversion and karyotypic frequencies through four different life-cycle stages: adult males, eggs, third-instar larvae, and immature adults. all population samples were obtained in june 1981 at an old opuntia ficus-indica plantation near carboneras, spain. the analysis rests on several assumptions which are explicitly set forth and discussed. the r ... | 1986 | 28556170 |
| pupal emergence pattern in cactophilic drosophila and the effect of host plants. | drosophila buzzatii and d. koepferae are sibling cactophilic species. the former breeds primarily on prickly pears (genus opuntia) whereas the latter breeds on columnar cacti of the genera cereus and trichocereus, although with certain degree of niche overlapping. we examined the interspecific differences in diurnal temporal patterns of adult emergence from puparia and evaluated whether this behavior is affected by rearing in the different cactus hosts available in nature. we detected important ... | 2017 | 28544122 |
| the role of courtship song in female mate choice in south american cactophilic drosophila. | courtship songs have undergone a spectacular diversification in the drosophila buzzatii cluster. accordingly, it has been suggested that sexual selection has played a significant role in promoting rapid diversification, reproductive isolation and speciation. however, there is no direct evidence (i.e., song playback experiments with wingless males) supporting this tenet. moreover, several studies have showed that the courtship song in the genus drosophila is not always used in female mate choice ... | 2017 | 28467464 |
| enhanced fertility and chill tolerance after cold-induced reproductive arrest in females of temperate species of the drosophila buzzatii complex. | long-term exposure to low temperatures during adult maturation might decrease fertility after cold recovery as a consequence of carry-over effects on reproductive tissues. this pattern should be more pronounced in tropical than in temperate species as protective mechanisms against chilling injuries are expected to be more effective in the latter. we initially determined the lower thermal thresholds to induce ovarian maturation in four closely related drosophila species, two inhabiting temperate ... | 2017 | 27956482 |
| transcriptome modulation during host shift is driven by secondary metabolites in desert drosophila. | high-throughput transcriptome studies are breaking new ground to investigate the responses that organisms deploy in alternative environments. nevertheless, much remains to be understood about the genetic basis of host plant adaptation. here, we investigate genome-wide expression in the fly drosophila buzzatii raised in different conditions. this species uses decaying tissues of cactus of the genus opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus t ... | 2016 | 27483442 |
| exploration of the drosophila buzzatii transposable element content suggests underestimation of repeats in drosophila genomes. | many new drosophila genomes have been sequenced in recent years using new-generation sequencing platforms and assembly methods. transposable elements (tes), being repetitive sequences, are often misassembled, especially in the genomes sequenced with short reads. consequently, the mobile fraction of many of the new genomes has not been analyzed in detail or compared with that of other genomes sequenced with different methods, which could shed light into the understanding of genome and te evolutio ... | 2016 | 27164953 |
| phenotypic plasticity in drosophila cactophilic species: the effect of competition, density, and breeding sites. | changes in the environmental conditions experienced by naturally occurring populations are frequently accompanied by changes in adaptive traits allowing the organism to cope with environmental unpredictability. phenotypic plasticity is a major aspect of adaptation and it has been involved in population dynamics of interacting species. in this study, phenotypic plasticity (i.e., environmental sensitivity) of morphological adaptive traits were analyzed in the cactophilic species drosophila buzzati ... | 2017 | 27061856 |
| first record of drosophila buzzatii (patterson & wheeler) (diptera: drosophilidae) emerging from a non-cactus host. | drosophila buzzatii (patterson & wheeler), a typical cactophilic species of the repleta group, is registered for the first time emerging from melon (cucumis melo) in western argentina. the analysis of inversion polymorphism and genetic diversity of mitochondrial cytochrome oxidase subunit i gene (mtcoi) provided additional evidence that corroborated the presence of a high proportion of d. buzzatii among the flies emerged from melon. this finding set the scenario for a broader range of possible h ... | 2016 | 26960546 |
| drosophila females undergo genome expansion after interspecific hybridization. | genome size (or c-value) can present a wide range of values among eukaryotes. this variation has been attributed to differences in the amplification and deletion of different noncoding repetitive sequences, particularly transposable elements (tes). tes can be activated under different stress conditions such as interspecific hybridization events, as described for several species of animals and plants. these massive transposition episodes can lead to considerable genome expansions that could ultim ... | 2016 | 26872773 |
| changes of osvaldo expression patterns in germline of male hybrids between the species drosophila buzzatii and drosophila koepferae. | hybridization between different genomes is a source of genomic instability, sometimes associated with transposable element (te) mobilization. previous work showed that hybridization between the species drosophila buzzatii and drosophila koepferae induced mobilization of different (tes), the osvaldo retrotransposon being the most unstable. however, we ignore the mechanisms involved in this transposition release in interspecific hybrids. in order to disentangle the mechanisms involved in this proc ... | 2015 | 25711309 |
| genomics of ecological adaptation in cactophilic drosophila. | cactophilic drosophila species provide a valuable model to study gene-environment interactions and ecological adaptation. drosophila buzzatii and drosophila mojavensis are two cactophilic species that belong to the repleta group, but have very different geographical distributions and primary host plants. to investigate the genomic basis of ecological adaptation, we sequenced the genome and developmental transcriptome of d. buzzatii and compared its gene content with that of d. mojavensis and two ... | 2014 | 25552534 |
| host alkaloids differentially affect developmental stability and wing vein canalization in cactophilic drosophila buzzatii. | host shifts cause drastic consequences on fitness in cactophilic species of drosophila. it has been argued that changes in the nutritional values accompanying host shifts may elicit these fitness responses, but they may also reflect the presence of potentially toxic secondary compounds that affect resource quality. recent studies reported that alkaloids extracted from the columnar cactus trichocereus terscheckii are toxic for the developing larvae of drosophila buzzatii. in this study, we tested ... | 2014 | 25366093 |
| selection at the esterase-2 locus of drosophila buzzatii? perturbation-reperturbation experiments. | apparent selection affecting starch gel electrophoretic alleles at the esterase-2 locus of drosophila buzzatii has been detected in laboratory and natural populations. perturbation-reperturbation of allele frequencies in replicated laboratory populations attempts to test direct selective effects at the locus versus effects of linked loci. sequential gel electrophoresis has identified more alleles within starch classes, and three of these alleles (within the a, b and c starch alleles) were used i ... | 2014 | 25250904 |
| structural and sequence diversity of the transposon galileo in the drosophila willistoni genome. | galileo is one of three members of the p superfamily of dna transposons. it was originally discovered in drosophila buzzatii, in which three segregating chromosomal inversions were shown to have been generated by ectopic recombination between galileo copies. subsequently, galileo was identified in six of 12 sequenced drosophila genomes, indicating its widespread distribution within this genus. galileo is strikingly abundant in drosophila willistoni, a neotropical species that is highly polymorph ... | 2014 | 25218200 |
| interspecific hybridization as a genomic stressor inducing mobilization of transposable elements in drosophila. | transposable elements (tes) are dna sequences able to be mobilized in host genomes. they are currently recognized as the major mutation inducers because of their insertion in the target, their effect on neighboring regions, or their ectopic recombination. a large number of factors including chemical and physical factors as well as intraspecific crosses have traditionally been identified as inducers of transposition. besides environmental factors, interspecific crosses have also been proposed as ... | 2014 | 25136509 |
| drosophila adult and larval pheromones modulate larval food choice. | insects use chemosensory cues to feed and mate. in drosophila, the effect of pheromones has been extensively investigated in adults, but rarely in larvae. the colonization of natural food sources by drosophila buzzatii and drosophila simulans species may depend on species-specific chemical cues left in the food by larvae and adults. we identified such chemicals in both species and measured their influence on larval food preference and puparation behaviour. we also tested compounds that varied be ... | 2014 | 24741012 |
| genome-wide dissection of hybrid sterility in drosophila confirms a polygenic threshold architecture. | to date, different studies about the genetic basis of hybrid male sterility (hms), a postzygotic reproductive barrier thoroughly investigated using drosophila species, have demonstrated that no single major gene can produce hybrid sterility without the cooperation of several genetic factors. early work using hybrids between drosophila koepferae (dk) and drosophila buzzatii (db) was consistent with the idea that hms requires the cooperation of several genetic factors, supporting a polygenic thres ... | 2014 | 24489077 |
| but2 is a member of the third major group of hat transposons and is involved in horizontal transfer events in the genus drosophila. | the hat superfamily comprises a large and diverse array of dna transposons found in all supergroups of eukaryotes. here we characterized the drosophila buzzatii but2 element and found that it harbors a five-exon gene encoding a 643-aa putatively functional transposase. a phylogeny built with 85 hat transposases yielded, in addition to the two major groups already described, ac and buster, a third one comprising 20 sequences that includes but2, tip100, hat-4_bm, and rp-hat1. this third group is h ... | 0 | 24459285 |
| quantitative genetic analysis of the body size and shape of drosophila buzzatii. | body size in drosophila is known to be closely related to a number of traits with important life history consequences, such as fecundity, dispersal ability and mating success. we examine the quantitative genetic basis of body size in three populations of the cactophilic species drosophila buzzatii, which inhabit climatically different areas of australia. flies were reared individually to eliminate any common environmental component in a full-sib design with families split between two temperature ... | 1993 | 24195935 |
| multiple paternity and sperm competition in the sibling species drosophila buzzatii and drosophila koepferae. | sperm competition (sc) is a major component of sexual selection that enhances intra- and intersexual conflicts and may trigger rapid adaptive evolution of sexual characters. the actual role of sc on rapid evolution, however, is poorly understood. besides, the relative contribution of distinctive features of the mating system to among species variation in the strength of sc remains unclear. here, we assessed the strength of sc and mating system factors that may account for it in the closely relat ... | 2013 | 23952173 |
| aedeagal divergence in sympatric populations of two sibling species of cactophilic drosophila (diptera: drosophilidae): evidence of character displacement? | aedeagal morphology of two sibling cactophilic species, drosophila buzzatii patterson & wheeler and drosophila koepferae fontdevila & wasserman, was analyzed in nine allopatric and three sympatric locations throughout south america. morphological differences were detected for both aedeagus size and shape between sympatric and allopatric populations of d. buzzatii, despite the significant variability within both groups. populations of d. buzzatii sympatric with d. koepferae displayed smaller aede ... | 2012 | 23950045 |
| effects of dietary composition on life span of drosophila buzzatii and its short-lived sibling species d. koepferae. | two sibling drosophila species dramatically divergent in longevity, drosophila buzzatii and d. koepferae, were examined for possible effects of both developmental culture medium and dietary composition (dc) on longevity. longevity was greatly increased in the longer lived d. buzzatii when flies were reared and fed on a rich-in-nutrient and cactus-based culture (r-cbc) as compared to longevity in a poor nutrient culture (pnc). in d. buzzatii, life span was further increased by exposing flies to s ... | 2013 | 23835870 |
| inter and intraspecific variation in female remating propensity in the cactophilic sibling species drosophila buzzatii and d. koepferae. | post-mating sexual selection by means of sperm competition or cryptic female choice occurs in species in which females remate before exhausting sperm supplied by previous mates. thus, sperm competition is expected to be stronger when inseminated females remate more frequently or take longer to deplete sperm load. previous studies comparing oviposition behavior in the pair of closely related species drosophila buzzatii and drosophila koepferae suggest that inseminated females of the latter deplet ... | 2013 | 23542152 |
| striking structural dynamism and nucleotide sequence variation of the transposon galileo in the genome of drosophila mojavensis. | 2013 | 23374229 | |
| chemical cues influence pupation behavior of drosophila simulans and drosophila buzzatii in nature and in the laboratory. | in the wild, larvae of several species of drosophila develop in heterogeneous and rapidly changing environments sharing resources as food and space. in this scenario, sensory systems contribute to detect, localize and recognize congeners and heterospecifics, and provide information about the availability of food and chemical features of environments where animals live. we investigated the behavior of d. simulans and d. buzzatii larvae to chemicals emitted by conspecific and heterospecific larvae ... | 2012 | 22737236 |
| segmental duplication, microinversion, and gene loss associated with a complex inversion breakpoint region in drosophila. | chromosomal inversions are usually portrayed as simple two-breakpoint rearrangements changing gene order but not gene number or structure. however, increasing evidence suggests that inversion breakpoints may often have a complex structure and entail gene duplications with potential functional consequences. here, we used a combination of different techniques to investigate the breakpoint structure and the functional consequences of a complex rearrangement fixed in drosophila buzzatii and comprisi ... | 2012 | 22328714 |
| effective population size of natural populations of drosophila buzzatii, with a comparative evaluation of nine methods of estimation. | allozyme and microsatellite data from numerous populations of drosophila buzzatii have been used (i) to determine to what degree n(e) varies among generations within populations, and among populations, and (ii) to evaluate the congruence of four temporal and five single-sample estimators of n(e) . effective size of different populations varied over two orders of magnitude, most populations are not temporally stable in genetic composition, and n(e) showed large variation over generations in some ... | 2011 | 21951766 |
| variations on a theme: diversification of cuticular hydrocarbons in a clade of cactophilic drosophila. | we characterized variation and chemical composition of epicuticular hydrocarbons (chcs) in the seven species of the drosophila buzzatii cluster with gas chromatography/mass spectrometry. despite the critical role of chcs in providing resistance to desiccation and involvement in communication, such as courtship behavior, mating, and aggregation, few studies have investigated how chc profiles evolve within and between species in a phylogenetic context. we analyzed quantitative differences in chc p ... | 2011 | 21699713 |
| adaptation of the aflp technique as a new tool to detect genetic instability and transposition in interspecific hybrids. | an adapted amplified fragment length polymorphism (aflp) protocol is presented for detection of hybrid instability in the genome of interspecific hybrids between drosophila buzzatii and d. koepferae species. analyses of 15 aflp instability markers (new bands detected in hybrids) show that up to 81% are the result of transposable element (te) activity. twenty tes associated with aflp instability markers have been detected by this method in backcross hybrids and segmental hybrids, demonstrating it ... | 2011 | 21548908 |
| osvaldo and isis retrotransposons as markers of the drosophila buzzatii colonisation in australia. | transposable elements (tes) constitute an important source of genetic variability owing to their jumping and regulatory properties, and are considered to drive species evolution. several factors that are able to induce te transposition in genomes have been documented (for example environmental stress and inter- and intra-specific crosses) but in many instances the reasons for te mobilisation have yet to be elucidated. colonising populations constitute an ideal model for studying te behaviour and ... | 2011 | 21513573 |
| local adaptation of stress related traits in drosophila buzzatii and drosophila simulans in spite of high gene flow. | we addressed the question if local adaptation to a thermal gradient is possible in spite of a high gene flow among closely spaced populations of two species of drosophila from the island of la gomera (canary islands). variation in multiple traits related to stress resistance in different life stages was measured in both species in flies collected from five localities at different altitudes and thereby with different climatic conditions. based on microsatellite loci, the populations were not gene ... | 2009 | 21462416 |
| testing chromosomal phylogenies and inversion breakpoint reuse in drosophila. the martensis cluster revisited. | the chromosomal relationships of the four martensis cluster species are among the most complex and intricate within the entire drosophila repleta group, due to the so-called sharing of inversions. here, we have revised these relationships using comparative mapping of bacterial artificial chromosome (bac) clones on the salivary gland chromosomes. a physical map of chromosome 2 of drosophila uniseta (one of the cluster members) was generated by in situ hybridization of 82 bac clones from the physi ... | 2011 | 21394512 |
| altitudinal and seasonal variation in microsatellite allele frequencies of drosophila buzzatii. | variation in climate, particularly temperature, is known to affect the genetic composition of populations. although there have been many studies of latitudinal variation, comparisons of populations across altitudes or seasons, particularly for animal species, are less common. here, we study genetic variation (microsatellite markers) in populations of drosophila buzzatii collected along altitudinal gradients and in different seasons. we found no differences in genetic variation between 2 years or ... | 2011 | 21091575 |
| geographic patterns of inversion polymorphism in the second chromosome of the cactophilic drosophila buzzatii from northeastern argentina. | the inversion polymorphisms of the cactophilic drosophila buzzatti patterson and wheeler (diptera: drosophilidae) were studied in new areas of its distribution in argentina. a total of thirty-eight natural populations, including 29 from previous studies, were analyzed using multiple regression analyses. the results showed that about 23% of total variation was accounted for by a multiple regression model in which only altitude contributed significantly to population variation, despite the fact th ... | 2010 | 21062144 |
| characterization and genomic organization of peri, a repetitive dna in the drosophila buzzatii cluster related to dine-1 transposable elements and highly abundant in the sex chromosomes. | transposable elements (tes) are dynamic components of eukaryotic genomes. we aimed to characterize tes to help elucidate their impact on the genomic architecture, diversity and evolution of chromosomes in the d. buzzatii cluster of species (repleta group). | 2011 | 20938165 |
| ontogenetic stage-dependent effect of temperature on developmental and metabolic rates in a holometabolous insect. | different hypotheses attempt to explain how different stages of organisms with complex life cycles respond to environmental changes. most studies have focused at the among-species level showing similar responses to temperature throughout ontogeny. however, there is no agreement about the pattern expected at the intraspecific scale where a strong selective effect is expected. in this paper, we studied the effects of thermal treatments on a life history trait (developmental rate) and a physiologic ... | 2010 | 20615413 |
| developmental acclimation affects clinal variation in stress resistance traits in drosophila buzzatii. | patterns of clinal genetic variation in drosophila are often characterized after rearing at constant temperatures. however, clinal patterns might change after acclimation if populations differ in their plastic response to fluctuating environments. we studied longevity, starvation and heat knock-down resistance after development at either constant or fluctuating temperatures in nine drosophila buzzatii populations collected along an altitudinal gradient in tenerife, spain. flies that developed at ... | 2010 | 20298441 |
| the transposon galileo generates natural chromosomal inversions in drosophila by ectopic recombination. | transposable elements (tes) are responsible for the generation of chromosomal inversions in several groups of organisms. however, in drosophila and other dipterans, where inversions are abundant both as intraspecific polymorphisms and interspecific fixed differences, the evidence for a role of tes is scarce. previous work revealed that the transposon galileo was involved in the generation of two polymorphic inversions of drosophila buzzatii. | 2009 | 19936241 |
| characterization of the shsp genes in drosophila buzzatii and association between the frequency of valine mutations in hsp23 and climatic variables along a longitudinal gradient in australia. | the small heat shock gene (shsp) cluster of drosophila buzzatii was sequenced and the gene order and dna sequence were compared with those of the shsps in drosophila melanogaster. the d. buzzatii shsp cluster contains an inversion and a duplication of hsp26. a phylogenetic tree was constructed based on hsp26 genes from several drosophila species of the sophophora and drosophila subgenera. the tree shows first a separation of the sophophora and the drosophila subgenera and then the drosophila sub ... | 2010 | 19806471 |
| evolutionary dynamics and sites of illegitimate recombination revealed in the interspersion and sequence junctions of two nonhomologous satellite dnas in cactophilic drosophila species. | satellite dna (satdna) is a major component of genomes but relatively little is known about the fine-scale organization of unrelated satdnas residing at the same chromosome location, and the sequence structure and dynamics of satdna junctions. we studied the organization and sequence junctions of two nonhomologous satdnas, pbum and dbc-150, in three species from the neotropical drosophila buzzatii cluster (repleta group). in situ hybridization to microchromosomes, interphase nuclei and extended ... | 2009 | 19259119 |
| cloning and sequencing of the breakpoint regions of inversion 5g fixed in drosophila buzzatii. | chromosomal inversions are ubiquitous in drosophila both as intraspecific polymorphisms and interspecific differences. many gaps still remain in our understanding of the mechanisms that generate them. previous work has shown that in drosophila buzzatii, three polymorphic inversions were generated by ectopic recombination between copies of the transposon galileo. in this study, we have characterized the breakpoint regions of inversion 5g, fixed in d. buzzatii and absent in drosophila koepferae an ... | 2009 | 19198866 |
| bottlenecks, population differentiation and apparent selection at microsatellite loci in australian drosophila buzzatii. | species colonizing new areas disjunct from their original habitat may be subject to novel selection pressures, and exhibit adaptive genetic changes. however, if colonization occurs through a small number of founders, the genetic composition of the colonized population may differ from that of the original population simply due to genetic drift. disentangling the effects of founder drift and selection after colonization is crucial to understanding the adaptive process. drosophila buzzatii colonize ... | 2009 | 19142202 |
| the foldback-like element galileo belongs to the p superfamily of dna transposons and is widespread within the drosophila genus. | galileo is the only transposable element (te) known to have generated natural chromosomal inversions in the genus drosophila. it was discovered in drosophila buzzatii and classified as a foldback-like element because of its long, internally repetitive, terminal inverted repeats (tirs) and lack of coding capacity. here, we characterized a seemingly complete copy of galileo from the d. buzzatii genome. it is 5,406 bp long, possesses 1,229-bp tirs, and encodes a 912-aa transposase similar to those ... | 2008 | 18287066 |
| sequence analysis, chromosomal distribution and long-range organization show that rapid turnover of new and old pbum satellite dna repeats leads to different patterns of variation in seven species of the drosophila buzzatii cluster. | we aimed to study patterns of variation and factors influencing the evolutionary dynamics of a satellite dna, pbum, in all seven drosophila species from the buzzatii cluster (repleta group). we analyzed 117 alpha pbum-1 (monomer length 190 bp) and 119 composite alpha/beta (370 bp) pbum-2 repeats and determined the chromosome location and long-range organization on dna fibers of major sequence variants. such combined methodologies in the study of satdnas have been used in very few organisms. in m ... | 2008 | 18266060 |
| wing morphology and fluctuating asymmetry depend on the host plant in cactophilic drosophila. | as in most insect groups, host plant shifts in cactophilic drosophila represent environmental challenges as flies must adjust their developmental programme to the presence of different chemical compounds and/or to a microflora that may differ in the diversity and abundance of yeasts and bacteria. in this context, wing morphology provides an excellent opportunity to investigate the factors that may induce changes during development. in this work, we investigated phenotypic plasticity and developm ... | 2008 | 18081744 |
| the impact of genetic parental distance on developmental stability and fitness in drosophila buzzatii. | measures of genetic parental distances (gpd) based on microsatellite loci (d(2) and ir), have been suggested to be better correlated with fitness than individual heterozygosity (h), as they contain information about past events of inbreeding or admixture. we investigated if gpd increased with increasing genetic divergence between parental populations in drosophila buzzatii and if the measures indicate past events of admixture. further we evaluated the relationship between gpd, fitness and fluctu ... | 2008 | 18060507 |
| a study of wing morphology and fluctuating asymmetry in interspecific hybrids between drosophila buzzatii and d. koepferae. | in this work we investigate the effect of interspecific hybridization on wing morphology using geometric morphometrics in the cactophilic sibling species d. buzzatii and d. koepferae. wing morphology in f1 hybrids exhibited an important degree of phenotypic plasticity and differs significantly from both parental species. however, the pattern of morphological variation between hybrids and the parental strains varied between wing size and wing shape, across rearing media, sexes, and crosses, sugge ... | 2008 | 17647081 |
| low rates of homogenization of the dbc-150 satellite dna family restricted to a single pair of microchromosomes in species from the drosophila buzzatii cluster. | a satellite dna family, termed dbc-150, comprises slightly gc-rich repeat units of approximately 150 bp that were isolated (by dna digestions or pcr) from the genome of all seven drosophila species from the buzzatii cluster (repleta group). the presence of subrepeats suggests that part of the extant dbc-150 monomer originated by the duplication of small sequence motifs. the dbc-150 family is compared to the previously described pbum satdna family, an abundant component of the genome of five spec ... | 2007 | 17551842 |
| evolution of male genitalia: environmental and genetic factors affect genital morphology in two drosophila sibling species and their hybrids. | the rapid evolution of genital morphology is a fascinating feature that accompanies many speciation events. however, the underlying patterns and explanatory processes remain to be settled. in this work we investigate the patterns of intraspecific variation and interspecific divergence in male genitalic morphology (size and shape) in the cactophilic sibling species drosophila buzzatii and d. koepferae. genital morphology in interspecific hybrids was examined and compared to the corresponding pare ... | 2007 | 17504529 |
| developmental time and size-related traits in drosophila buzzatii along an altitudinal gradient from argentina. | clinal analysis for fitness-related traits provides a well-known approach to investigate adaptive evolution. several fitness-related traits (developmental time, thorax length, wing length and wing loading) were measured at two laboratory generations (g7 and g33) of d. buzzatii from an altitudinal gradient from northwestern argentina, where significant thermal differences persist. developmental time (dt) was positively correlated with altitude of origin of population. further, dt was negatively c ... | 2006 | 17362338 |
| the evolutionary history of drosophila buzzatii. xii. the genetic basis of sterility in hybrids between d. buzzatii and its sibling d. serido from argentina. | the genetic basis of hybrid sterility has been investigated in backcross segmental hybrids between two sibling species, drosophila buzzatii and d. serido. asynapsis of homologous bands in hybrid polytene chromosomes has been used to identify the d. serido chromosome segments introgressed into the d. buzzatti genome. all the investigated chromosomes contain male sterility factors. for autosomes, sterility is produced when an introgressed d. serido chromosome segment, or combination of segments, r ... | 1986 | 17246354 |
| the evolutionary history of drosophila buzzatii. iii. cytogenetic relationships between two sibling species of the buzzatii cluster. | drosophila buzzatii has been found sympatric in argentina with a closely-related sibling species, d. serido. the biogeographical, reproductive and chromosomal data allow us to combine these species into an evolutionary unit, the buzzatii cluster. salivary gland chromosomes also have been used to determine their phylogenetic relationships with other closely related species, showing that the buzzatii cluster species share two inversions-2d(2) and 2s(6)-with the species of the martensis cluster. bo ... | 1982 | 17246089 |
| molecular population genetics of the alpha-esterase5 gene locus in original and colonized populations of drosophila buzzatii and its sibling drosophila koepferae. | several studies have suggested that esterase-2 (est-2) may be the target of natural selection in the cactophilic fly drosophila buzzatii. in this work, we analyzed nucleotide variation in a fragment of alpha-esterase5 (alphae5), the gene encoding est-2, in original (argentinian) and colonized (australian) populations of d. buzzatii and in its sibling d. koepferae. estimates of nucleotide heterozygosity in d. buzzatii were similar in australia and argentina, although we detected a loss of singlet ... | 2007 | 17160646 |
| the evolutionary history of drosophila buzzatii. xxxvi. molecular structural analysis of osvaldo retrotransposon insertions in colonizing populations unveils drift effects in founder events. | previous work on transposable element distribution in colonizing populations of drosophila buzzatii revealed a high frequency of occupancy in several chromosomal sites. two explanatory hypotheses were advanced: the founder hypothesis, by which founder genetic drift was responsible, and the unstable hypothesis that assigns this unusual distribution to bursts of transposition toward some chromosomal sites. here, we study the molecular structure of three euchromatic osvaldo clones isolated from sit ... | 2007 | 17151248 |
| molecular characterization and genomic distribution of isis: a new retrotransposon of drosophila buzzatii. | a new transposable element, isis, is identified as a ltr retrotransposon in drosophila buzzatii. dna sequence analysis shows that isis contains three long orfs similar to gag, pol and env genes of retroviruses. the orf1 exhibits sequence homology to matrix, capsid and nucleocapsid gag proteins and orf2 encodes a putative protease (pr), a reverse transcriptase (rt), an rnase h (rh) and an integrase (in) region. the analysis of a putative env product, encoded by the env orf3, shows a degenerated p ... | 2007 | 17039376 |
| conservation of pbum-2 satellite dna sequences among geographically isolated drosophila gouveai populations from brazil. | in this study, we have compared 34 repetition units of pbum-2 satellite dna of individuals from six isolated populations of drosophila gouveai, a cactophilic member of drosophila buzzatii cluster (repleta group). in contrast to the results of previous morphological and molecular data, which suggest differentiation among the d. gouveai populations, the sequences and the cluster analysis of pbum-2 monomers showed that this repetitive element is highly conserved among the six d. gouveai populations ... | 2006 | 17028958 |
| altitudinal patterns for longevity, fecundity and senescence in drosophila buzzatii. | we tested for variation in longevity, senescence rate and early fecundity of drosophila buzzatii along an elevational transect in argentina, using laboratory-reared flies in laboratory tests performed to avoid extrinsic mortality. at 25 degrees c, females from lowland populations lived longer and had a lower demographic rate of senescence than females from highland populations. minimal instead of maximal temperature at the sites of origin of population best predicted this cline. a very different ... | 2006 | 17028942 |
| testing chromosomal phylogenies and inversion breakpoint reuse in drosophila. | a combination of cytogenetic and bioinformatic procedures was used to test the chromosomal phylogeny relating drosophila buzzatii with d. repleta. chromosomes x and 2, harboring most of the inversions fixed between these two species, were analyzed. first, chromosomal segments conserved during the divergence of the two species were identified by comparative in situ hybridization to the d. repleta chromosomes of 180 bac clones from a bac-based physical map of the d. buzzatii genome. these conserve ... | 2007 | 17028333 |
| longevity differences among lines artificially selected for developmental time and wing length in drosophila buzzatii. | we assessed the indirect response of longevity in lines selected for wing length (wl) and developmental time (dt). longevity in selection lines was compared to laboratory control lines and the offspring of recently collected females. wild flies (w lines), flies from lines selected for fast development (f lines), and for fast development and large wing length (l lines) outlived control laboratory lines (c lines) and lines selected for fast development and short wing (s lines). the decline in long ... | 2006 | 16850224 |
| patterns of variation in wing morphology in the cactophilic drosophila buzzatii and its sibling d. koepferae. | drosophila buzzatii and d. koepferae are two sibling species that breed on the necrotic tissues of several cactus species and show a certain degree of niche overlap. also, they show differences in several life history traits, such as body size and developmental time, which probably evolved as a consequence of adaptation to different host plants. in this work we investigate the ecological and genetic factors affecting wing morphology variation both within and between species. three wing traits we ... | 2006 | 16780528 |
| abundance and chromosomal distribution of six drosophila buzzatii transposons: but1, but2, but3, but4, but5, and but6. | the abundance and chromosomal distribution of six class-ii transposable elements (tes) of drosophila buzzatii have been analyzed by southern blotting and in situ hybridization. these six transposons had been previously found at the breakpoints of inversions 2j and 2q ( 7 ) of d. buzzatii. these two polymorphic inversions were generated by an ectopic recombination event between two copies of galileo, a foldback element. the four breakpoints became hotspots for te insertions after the generation o ... | 2006 | 16773395 |
| aedeagus morphology as a discriminant marker in two closely related cactophilic species of drosophila (diptera; drosophilidae) in south america. | drosophila serido and d. antonietae are sibling species belonging to the drosophila buzzatii cluster. morphologically, they can only be discriminated by quantitative traits. in this paper we analyze the length and equalized average curvature of four regions of the aedeagus of d. antonietae and d. serido. specimens of d. serido and d. antonietae were classified correctly 96.74% of the time. based only on the variable that most contributed to the discrimination of the groups (equalized average cur ... | 2006 | 16710560 |
| reproductive relationships and degree of synapsis in the polytene chromosomes of the drosophila buzzatii species cluster. | the process of speciation occurs through the evolution of any of several forms of reproductive isolation between taxa, including inviability of hybrids. in this work, strains derived from allopatric populations of drosophila buzzatii cluster species were experimentally crossed in order to evaluate their reproductive and cytogenetic relationships, and to contribute toward understanding the reproductive isolation in this group of sibling species. although intrastrain crosses were highly fertile, w ... | 2006 | 16710520 |
| genotype by environment interactions in viability and developmental time in populations of cactophilic drosophila. | the genetic and ecological basis of viability and developmental time differences between drosophila buzzatii and d. koepferae were analysed using the isofemale line technique. several isofemale lines were sampled from pairs of allopatric/sympatric populations of each species. flies were reared in media prepared with decaying tissues of two of the main natural cactus hosts of each species. this experimental design enabled us to evaluate the relative contribution of phenotypic plasticity, genetic ... | 2006 | 16674586 |
| climatic adaptation of drosophila buzzatii populations in southeast australia. | variation in 19 traits possibly relevant for thermal adaptation was studied in 11 populations of drosophila buzzatii collected in southeast australia. using stepwise multiple regression, the variation was compared to variation in geographic coordinates and to a set of climatic variables estimated for each collection site. for 13 of the traits, a significant part of the variation was explained by climatic variables and/or geographic coordinates, suggesting directional selection for adaptation to ... | 2006 | 16622471 |
| cactophilic drosophila in south america: a model for evolutionary studies. | the drosophila buzzatii cluster is composed of seven cactophilic species and their known geographical distribution encompasses the open vegetation diagonal, which includes the morphoclimatic domains of the caatinga, chaco and cerrado, which are situated between the amazon and the atlantic forests. besides these areas, these cactophilic species are also found in a narrow strip along the atlantic coast from northeastern brazil to the southern tip of the country. the hypothesis of vicariant events, ... | 2006 | 16502085 |
| altitudinal variation for stress resistance traits and thermal adaptation in adult drosophila buzzatii from the new world. | multiple stress resistance traits were investigated in the cactophilic fly drosophila buzzatii. adults from seven populations derived from north-western argentina were compared with respect to traits relevant for thermal stress resistance and for resistance to other forms of environmental stress. the populations were collected along an altitudinal gradient spanning more than 2000 m in height, showing large climatic differences. the results suggest that knock-down resistance to heat stress, desic ... | 2005 | 16033554 |
| phylogeny and molecular evolution of the drosophila hydei subgroup (drosophila repleta group) inferred from the xanthine dehydrogenase gene. | the hydei subgroup (drosophila repleta group) consists of seven species divided into two complexes: bifurca and hydei, whose phylogenetic relationships are not well understood. to evaluate the molecular phylogeny of this subgroup, we analyzed 2085 bp of coding sequence of the xanthine dehydrogenase gene in six available species of the hydei subgroup, with drosophila buzzatii and drosophila mulleri as an outgroup. for phylogenetic reconstruction we adopted a maximum-likelihood framework, based on ... | 2005 | 15935705 |
| a bac-based physical map of the drosophila buzzatii genome. | large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps, and provide useful resources for sequencing entire genomes. drosophila buzzatii is a representative species of the repleta group in the drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation, and speciation. we constructed a bacterial artificial chromosome (bac) genomic library of d. buzzatii using the shuttle v ... | 2005 | 15930498 |
| conservation of regulatory sequences and gene expression patterns in the disintegrating drosophila hox gene complex. | homeotic (hox) genes are usually clustered and arranged in the same order as they are expressed along the anteroposterior body axis of metazoans. the mechanistic explanation for this colinearity has been elusive, and it may well be that a single and universal cause does not exist. the hox-gene complex (hom-c) has been rearranged differently in several drosophila species, producing a striking diversity of hox gene organizations. we investigated the genomic and functional consequences of the two h ... | 0 | 15867430 |
| evolutionary turnover of two pbum satellite dna subfamilies in the drosophila buzzatii species cluster (repleta group): from alpha to alpha/beta arrays. | the pbum satellite dna family was studied in seven drosophila species from the buzzatii cluster (within the large drosophila repleta group). the pbum repeats are slightly at-rich and show high levels of intraspecific sequence homogeneity. the pbum family can be divided into two subfamilies. the pbum-1 subfamily consists of tandemly arranged repetition units of approximately 190 bp, termed alpha. alpha repeats were found in a high copy number in the genome of d. buzzatii, d. serido and d. antonie ... | 2005 | 15777676 |
| molecular characterization and chromosomal distribution of galileo, kepler and newton, three foldback transposable elements of the drosophila buzzatii species complex. | galileo is a foldback transposable element that has been implicated in the generation of two polymorphic chromosomal inversions in drosophila buzzatii. analysis of the inversion breakpoints led to the discovery of two additional elements, called kepler and newton, sharing sequence and structural similarities with galileo. here, we describe in detail the molecular structure of these three elements, on the basis of the 13 copies found at the inversion breakpoints plus 10 additional copies isolated ... | 2005 | 15695364 |
| silencing of a gene adjacent to the breakpoint of a widespread drosophila inversion by a transposon-induced antisense rna. | adaptive changes in nature occur by a variety of mechanisms, and drosophila chromosomal inversions was one of the first studied examples. however, the precise genetic causes of the adaptive value of inversions remain uncertain. here we investigate the impact of the widespread inversion 2j of drosophila buzzatii on the expression of the cg13617 gene, whose coding region is located only 12 bp away from the inversion proximal breakpoint. this gene is transcribed into a 2.3-kb mrna present in all d. ... | 2004 | 15184654 |
| on the pbum189 satellite dna variability among south american populations of drosophila buzzatii. | the pbum189 satellite dna was analysed in drosophila buzzatii populations that cover most of the species distribution in south america. this satdna consists of a + t-rich monomers of 189 bp and previous studies showed a fast rate of evolutionary change of this component of d. buzzatii genome. a total of 63 pbum189 repetition units from 14 d. buzzatii populations (9 from brazil and 5 from argentina) were studied. the average nucleotide variability among the 63 repeats is 4.2 %. at least one repea ... | 2003 | 15061796 |
| remating and sperm displacement in a natural population of drosophila buzzatii inferred from mother-offspring analysis of microsatellite loci. | prospects for estimation of parameters of models of sperm competition from field data have improved recently with the development of methods that employ multilocus genotype data from brood-structured samples. sperm competition in drosophila buzzatii is of special interest because it is possible to directly observe the breeding behaviour of this species in its natural habitat of rotting cactus. previous laboratory experiments showed that this species exhibits an unusual pattern of frequent remati ... | 2004 | 15009271 |
| heat and cold-induced male sterility in drosophila buzzatii: genetic variation among populations for the duration of sterility. | here we studied three phenotypic traits in drosophila buzzatii that are strongly effected by temperature, and are expected to be closely associated with fitness in nature. the traits measured were thermal threshold of male sterility, time for males to gain fertility when reared at a sterility-inducing temperature and transferred to 25 degrees c on eclosion and survival after development. the last two traits were measured under four temperature regimes, constant 12 degrees c, 25 degrees c, 31 deg ... | 2004 | 14679393 |
| comparative molecular population genetics of the xdh locus in the cactophilic sibling species drosophila buzzatii and d. koepferae. | the xdh (rosy) gene is one of the best studied in the drosophila genus from an evolutionary viewpoint. here we analyze nucleotide variation in a 1875-bp fragment of the second exon of xdh in argentinian populations of the cactophilic d. buzzatii and its sibling d. koepferae. the major electrophoretic alleles of d. buzzatii not only lack diagnostic amino acids in the region studied but also differ on average from each other by four to 13 amino acid changes. our data also suggest that d. buzzatii ... | 2004 | 14595098 |
| chromosomal inversions effect body size and shape in different breeding resources in drosophila buzzatii. | the cactophilic drosophila buzzatii provides an excellent model for the study of reaction norms across discrete environments because it breeds on rotting tissues (rots) of very different cactus species. here we test the possible effects of second chromosome inversions on body size and shape (wing loading) across suitable natural breeding substrates. using homokaryotypic stocks derived from several lines homozygous for four naturally occurring chromosomal inversions, we show that arrangements sig ... | 2003 | 12815453 |
| the evolutionary history of drosophila buzzatii. xxxv. inversion polymorphism and nucleotide variability in different regions of the second chromosome. | inversions are portions of a chromosome where the gene order is reversed relative to a standard reference orientation. because of reduced levels of recombination in heterokaryotypes, inversions have a potentially important effect on patterns of nucleotide variability in those genomic regions close to, or included in, the inverted fragments. here we report sequence variation at three anonymous regions (stss) located at different positions in relation to second-chromosome inversion breakpoints in ... | 2003 | 12716995 |
| the foldback-like transposon galileo is involved in the generation of two different natural chromosomal inversions of drosophila buzzatii. | chromosomal inversions are the most common type of genome rearrangement in the genus drosophila. although the potential of transposable elements (tes) for generating inversions has been repeatedly demonstrated in the laboratory, little is known on their role in the generation of natural inversions, which are those effectively contributing to the adaptation and/or evolution of species. we have cloned and sequenced the two breakpoints of the polymorphic inversion 2q7 of d. buzzatii. the sequence a ... | 2003 | 12679549 |
| evaluation of the courtship and of the hybrid male sterility among drosophila buzzatii cluster species (diptera, drosophilidae). | in the drosophila repleta group the establishment of subgroups and complexes made on the basis of morphological and cytological evidences is supported by tests of reproductive isolation. among species in the repleta group, the buzzatii cluster, due to its polymorphism and polytipism, is an excellent material for ecological and speciation studies. some interspecific crosses involving drosophila seriema, drosophila sp. b, d. koepferae and d. buzzatii strains were completely sterile while others in ... | 2002 | 12659009 |
| transpecific polymorphisms in an inversion linked esterase locus in drosophila buzzatii. | nucleotide variation was studied in a 1.1 kb section of the coding region of an esterase gene (est-a) that maps in the center of the segments rearranged by polymorphic inversions in the cactophilic drosophila buzzatii. we examine 30 homozygous second-chromosome lines differing in gene arrangement and three d. koepferae isofemale lines as outgroups. our data show that est-a is a highly polymorphic gene at both synonymous and replacement sites. significant departures from homogeneity in the distri ... | 2003 | 12644562 |
| direct and correlated responses to artificial selection on developmental time and wing length in drosophila buzzatii. | developmental time and body size are two positively correlated traits closely related to fitness in many organisms including drosophila. previous work suggested that these two traits are involved in a trade-off that may result from a negative genetic correlation between their effects on pre-adult and adult fitness. here, we examine the evolution of developmental time and body size (indexed by wing length) under artificial selection applied to one or both traits in replicated d. buzzatii populati ... | 2002 | 12583594 |
| the evolutionary history of drosophila buzzatii. xxxiv. the distribution of the retrotransposon osvaldo in original and colonizing populations. | the frequency distribution of the retrotransposon osvaldo in the haploid genome of drosophila buzzatii has been studied in five natural populations from the iberian peninsula and six natural populations from argentina. in iberian populations, osvaldo insertion sites do not follow a poisson distribution, most probably due to eight euchromatic sites with high occupancy, found in all populations. the estimated alpha and beta parameters, which measure the relative importance of drift and negative se ... | 1998 | 12572617 |
| developmental time, body size and wing loading in drosophila buzzatii from lowland and highland populations in argentina. | genotype-by-temperature interaction is a necessary condition for adaptive evolution of fitness traits as a response to temperature. several fitness-related traits (developmental time, pre-adult survival, thorax and wing lengths, and wing loading) were measured in laboratory-reared d. buzzatii from four populations sampled at different altitudes in north-western argentina: a lowland population (407 m a.s.l.), two populations from intermediate altitude (780 to 950 m a.s.l.), and a highland populat ... | 2001 | 12035612 |
| genetic variation in original and colonizing drosophila buzzatii populations analysed by microsatellite loci isolated with a new pcr screening method. | a new polymerase chain reaction-based screening method for microsatellites is presented. using this method, we isolated 12 microsatellite loci from drosophila buzzatii, two of which were x-linked. we applied the other 10 microsatellite loci to the analysis of genetic variation in five natural populations of d. buzzatii. two populations were from the species' original distribution in argentina, whereas the other three were from europe (two) and australia that were colonized 200 and 65 years ago, ... | 2002 | 11856420 |
| oviposition acceptance and fecundity schedule in the cactophilic sibling species drosophila buzzatii and d. koepferae on their natural hosts. | we tested for the occurrence of oviposition acceptance for different media prepared with cactus tissues of three alternative cactus hosts: opuntia sulphurea, o. quimilo and trichocereus terschekii for 4 consecutive days in lines of two drosophila buzzatii populations and one population of d. koepferae. our results showed that the former laid significantly more eggs on both opuntia cacti than on t. terschekii, whereas d. koepferae preferred t. terschekii. in addition, fecundity schedules differed ... | 2001 | 11831675 |
| fluctuating asymmetry is nongenetically related to mating success in drosophila buzzatii. | to date, there is still no consensus on the real significance of fluctuating asymmetry (fa) in evolutionary biology. some studies have established links between fa and darwinian fitness, and in a number of cases intermediate heritabilities for fa have been reported. however, many claims have been raised against the generality of these findings. i therefore tested if fa of a sexually selected trait (wing length) is indeed related to male mating success in drosophila buzzatii from field and labora ... | 2001 | 11794784 |
| gametic associations between inversion and allozyme polymorphisms in drosophila buzzatii. | gametic disequilibria between second chromosome polymorphic arrangements and seven linked allozyme loci were estimated in seven populations of drosophila buzzatii from argentina. significant and consistent associations across populations were detected for est-1, est-2, aldox, and xdh: phenograms based on nei's genetic distance showed that chromosomes carrying the 2st arrangement were more similar to each other, irrespective of the population from which they were extracted, than to chromosomes ca ... | 2001 | 11773244 |