Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| rhipicephalus microplus (acarina, ixodidae) in angola: evidence of its establishment and expansion. | livestock constitutes a fundamental asset for the livelihood of rural communities in angola. rhipicephalus microplus (acarina, ixodidae) is considered to be the most important external parasite impacting the cattle industry in the world. the present work used a cross-sectional study on tick species infesting cattle in smallholder and commercial farms, to investigate the presence of r. microplus in angola. this species was found to be recently established in certain areas of cuanza-sul and bengue ... | 2018 | 29380168 |
| metarhizium anisopliae s.l. modulation of lipid metabolism during tick infection is independent of ampk and erk pathways. | despite the importance of fat body in metabolism of arthropods, studies in ticks are scarce. this study evaluated the lipid composition and activation of extracellular signal-regulated protein kinase (erk) and amp-activated protein kinase (ampk) enzymes in rhipicephalus microplus fat body after infection with different isolates of the fungus metarhizium anisopliae sensu lato (metschnikoff, 1879) sorokin, 1883. the isolates cg 32, gc 112, gc 148, gc 347, and gc 629 were inoculated as viable or no ... | 2018 | 29352348 |
| a molecular survey of tick-borne pathogens from ticks collected in central queensland, australia. | central queensland (cq) is a large and isolated, low population density, remote tropical region of australia with a varied environment. the region has a diverse fauna and several species of ticks that feed upon that fauna. this study examined 518 individual ticks: 177 rhipicephalus sanguineus (brown dog tick), 123 haemaphysalis bancrofti (wallaby tick), 102 rhipicephalus australis (australian cattle tick), 47 amblyomma triguttatum (ornate kangaroo tick), 57 ixodes holocyclus (paralysis tick), 9 ... | 2018 | 29336689 |
| estimates of repeatability and correlations of hemoparasites infection levels for cattle reared in endemic areas for rhipicephalus microplus. | rhipicephalus microplus is a vector of cattle tick fever, a disease caused by the protozoans babesia bovisand b. bigemina, and also anaplasmosis, produced by the rickettsiales anaplasma marginale. these tick-borne pathogens cause considerable losses to brazilian livestock breeders and represent an obstacle to the expanded use of taurine breeds due to their higher sensitivity to ticks and hemoparasites compared to zebu breeds. differences in the susceptibility to hemoparasites were also verified ... | 2018 | 29329629 |
| determination and validation of discriminating concentration of ivermectin against rhipicephalus microplus. | rhipicephalus microplus, the major cattle tick species of india is prevalent all over the country and causes huge economic loss directly or indirectly to the dairy industries. chemical acaricides are playing an important role in managing tick infestations on livestock for many years and consequently, resistance to commonly used organophosphate (op) and synthetic pyrethroid (sp) compounds has been reported. subsequently, ivermectin (ivm) has been emerged as an alternative to manage op and sp resi ... | 2018 | 29329620 |
| population structure and genetic diversity of rhipicephalus microplus in zimbabwe. | recently there was an expansion in the geographic range of rhipicephalus microplus in zimbabwe. in order to understand gene flow patterns and population structure in this highly invasive and adaptable cattle tick, a population genetics study was carried out. eighty-seven r. microplus tick samples drawn from 5 distinct populations were genotyped using eight polymorphic microsatellite loci. genetic diversity (he) was high (0.755-0.802) in all the populations, suggesting high levels of gene flow wi ... | 2018 | 29325969 |
| cattle tick rhipicephalus microplus-host interface: a review of resistant and susceptible host responses. | ticks are able to transmit tick-borne infectious agents to vertebrate hosts which cause major constraints to public and livestock health. the costs associated with mortality, relapse, treatments, and decreased production yields are economically significant. ticks adapted to a hematophagous existence after the vertebrate hemostatic system evolved into a multi-layered defense system against foreign invasion (pathogens and ectoparasites), blood loss, and immune responses. subsequently, ticks evolve ... | 2017 | 29322033 |
| local immune response to larvae of rhipicephalus microplus in santa gertrudis cattle immune response against rhipicephalus microplus. | this study investigated the local immune response at larval attachment sites in santa gertrudis cattle with low and high levels of tick resistance. | 2018 | 29314141 |
| tick-host range adaptation: changes in protein profiles in unfed adult ixodes scapularis and amblyomma americanum saliva stimulated to feed on different hosts. | understanding the molecular basis of how ticks adapt to feed on different animal hosts is central to understanding tick and tick-borne disease (tbd) epidemiology. there is evidence that ticks differentially express specific sets of genes when stimulated to start feeding. this study was initiated to investigate if ticks such as ixodes scapularis and amblyomma americanum that are adapted to feed on multiple hosts utilized the same sets of proteins to prepare for feeding. we exposed i. scapularis a ... | 2017 | 29312895 |
| prevalence, risk factors, and genetic diversity of veterinary important tick-borne pathogens in cattle from rhipicephalus microplus-invaded and non-invaded areas of benin. | babesiosis, theileriosis, anaplasmosis, and heartwater are tick-borne diseases (tbd) that threaten livestock production in sub-saharan africa including benin. this country has been faced with an invasion of rhipicephalus microplus, a major vector for babesiosis, theileriosis, and anaplasmosis over the last decade. yet, data on tbd and the impact of the invasive ticks are lacking, making risk level evaluation and disease control arduous. in this study, epidemiological features of babesia bovis, b ... | 2017 | 29307783 |
| immunological control of ticks and tick-borne diseases that impact cattle health and production. | the cattle industry is one of the most important agroeconomic activities in mexico. the national herd is estimated to include approximately 33.5. million head of cattle. ticks and tick-borne diseases are principal factors with a negative impact on cattle health and production. the most economically important tick species parasitizing cattle in mexico are rhipicephalus microplus, r. annulatus, and amblyomma mixtum. parasitism by ticks affects cattle health and production directly. morbidity and m ... | 2018 | 29293449 |
| a soluble inorganic pyrophosphatase from the cattle tick rhipicephalus microplus capable of hydrolysing polyphosphates. | polyphosphates have been found in all cell types examined to date and play diverse roles depending on the cell type. in eukaryotic organisms, polyphosphates have been investigated mainly in mammalian cells, and only a few studies have addressed arthropods. pyrophosphatases have been shown to regulate polyphosphate metabolism. however, these studies were restricted to trypanosomatids. here we focus on the tick rhipicephalus microplus, a haematophagous ectoparasite that is highly harmful to cattle ... | 2017 | 29271528 |
| immune-related redox metabolism of embryonic cells of the tick rhipicephalus microplus (bme26) in response to infection with anaplasma marginale. | it is well known that reactive oxygen species (ros) and reactive nitrogen species (rns) are involved in the control of pathogens and microbiota in insects. however, the knowledge of the role of ros and rns in tick-pathogen and tick-microbiota interactions is limited. here, we evaluated the immune-related redox metabolism of the embryonic cell line bme26 from the cattle tick rhipicephalus microplus in response to anaplasma marginale infection. | 2017 | 29258559 |
| prevalence, intensity and population dynamics of hard ticks (acari: ixodidae) on sheep in the humid tropics of mexico. | hard ticks are a perennial problem in livestock systems throughout mexico. no data are currently available on tick prevalence, infestation intensity and population dynamics for sheep in the humid tropics of mexico. blackbelly sheep (n = 30) in tabasco state, mexico, were examined every 3 weeks for 1 year, and all ixodidae ticks were counted and removed for analysis. tick species were identified, infestation prevalence and intensity calculated per animal, and infestation per body zone determined. ... | 2017 | 29255964 |
| a coxiella mutualist symbiont is essential to the development of rhipicephalus microplus. | the cattle tick rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. different species of ticks harbor a symbiont bacterium of the genus coxiella. it was showed that a coxiella endosymbiont from r. microplus (cerm) is a vertically transmitted mutualist symbiont, comprising 98% of the 16s rrna sequences in both eggs and larvae. sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic c ... | 2017 | 29242567 |
| range expansion of the economically important asiatic blue tick, <i>rhipicephalus microplus</i>, in south africa. | the asiatic blue tick, rhipicephalus microplus, a known vector of bovine babesiosis and bovine anaplasmosis, is of great concern in the cattle industry. for this reason, detailed knowledge of the distribution of r. microplus is vital. currently, r. microplus is believed to be associated mainly with the northern and eastern savanna and grassland vegetation in south africa. the objective of the study was to record the distribution of r. microplus, and the related endemic rhipicephalus decoloratus, ... | 2017 | 29227140 |
| ehrlichia and anaplasma infections: serological evidence and tick surveillance in peninsular malaysia. | little information is available on human anaplasmosis and ehrlichiosis in southeast asia despite increasing reports of the detection of anaplasma spp. and ehrlichia spp. in the ticks. we report herein the serological findings against the tick-borne pathogens in a group of animal farm workers (n = 87) and indigenous people (n = 102) in peninsular malaysia. igg antibodies against ehrlichia chaffeensis were detected from 29.9% and 34.3% of farm workers and indigenous people, respectively, using com ... | 2017 | 29202206 |
| historical review and insights on the livestock tick-borne disease research of a developing country: the philippine scenario. | tick-borne diseases (tbds) remain to be a global animal health threat. developing countries like the philippines is not exempt to this. despite the potential impact tbds can give to these countries, local government initiatives and researches remain to be limited. in the philippines, most epidemiological studies were confined only to specific areas, and predominantly in the northern area. due to its unique geography and limited studies, the current nationwide status of most tbds could not be cle ... | 2017 | 29197596 |
| relative transcription of autophagy-related genes in amblyomma sculptum and rhipicephalus microplus ticks. | ticks endure stressful off-host periods and perform as vectors of a diversity of infectious agents, thus engaging pathways that expectedly demand for autophagy. little is known of ticks' autophagy, a conserved eukaryotic machinery assisting in homeostasis processes that also participates in tissue-dependent metabolic functions. here, the autophagy-related atg4 (autophagin-1), atg6 (beclin-1) and atg8 (lc3) mrnas from the human diseases vector amblyomma sculptum and the cattle-tick rhipicephalus ... | 2017 | 29181673 |
| detection of leishmania (v) guyanensis in rhipicephalus (boophilus) microplus (acari: ixodidae) collected from pecari tajacu. | previous studies identified the presence of leishmania infantum in rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts. | 2017 | 29161493 |
| bovine babesiosis: cattle protected in the field with a frozen vaccine containing babesia bovis and babesia bigemina cultured in vitro with a serum-free medium. | an attenuated live vaccine containing babesia bovis and b. bigemina cultured in vitro with a serum-free medium was assessed for its clinical protection conferred of naïve cattle, under natural tick-challenge in a high endemicity zone to babesia spp. three groups of six animals were treated as follows: group i (gi) received a vaccine derived from parasites cultured with a free-serum medium; group ii (gii) were immunized with the standard vaccine, with parasites cultured in a medium supplemented w ... | 2017 | 29155165 |
| strategies for the control of rhipicephalus microplus ticks in a world of conventional acaricide and macrocyclic lactone resistance. | infestations with the cattle tick, rhipicephalus microplus, constitute the most important ectoparasite problem for cattle production in tropical and subtropical regions worldwide, resulting in major economic losses. the control of r. microplus is mostly based on the use of conventional acaricides and macrocyclic lactones. however, the intensive use of such compounds has resulted in tick populations that exhibit resistance to all major acaricide chemical classes. consequently, there is a need for ... | 2018 | 29152691 |
| seasonal analysis and acaricidal activity of the thymol-type essential oil of ocimum gratissimum and its major constituents against rhipicephalus microplus (acari: ixodidae). | the tick rhipicephalus microplus affects cattle health, with production loss in tropical and subtropical regions. moreover, the use of commercial acaricides has been reduced due to the resistance of this parasite. although alternatives such as plant bioactive molecules have been sought, essential oils present variations in their chemical constituents due to environmental factors, which can interfere with their acaricidal activity. the objective of the present study was to evaluate the seasonal i ... | 2018 | 29152690 |
| characterization of a glycine-rich protein from rhipicephalus microplus: tissue expression, gene silencing and immune recognition. | salivary molecules, as glycine-rich proteins (grps), are essential to tick attachment and feeding on the host and are suggested to be involved in the host's immune system evasion, therefore representing natural candidates in the search for protective vaccine antigens. this work shows the molecular characterization of a grp from rhipicephalus microplus (rmgrp). the cdna and putative amino acid sequences were analysed, as well as the transcription level in tick tissues/developmental stages, showin ... | 2017 | 29144218 |
| mycoplasma ovis infection in goat farms from northeastern brazil. | although mycoplasma ovis (formerly eperythrozoon ovis) has been described in small ruminants worldwide, data on m. ovis in goats remain scarce. accordingly, the aims of the present study were to i) determine the prevalence of hemoplasmas in goats, ii) identify the tick species parasitizing the animals, and iii) determine factors associated with infection in five dairy and three beef goat farms from the paraíba state, northeastern brazil. blood samples were obtained from 402 goats. samples were s ... | 2017 | 29127988 |
| in vitro evaluation of essential oils derived from piper nigrum (piperaceae) and citrus limonum (rutaceae) against the tick rhipicephalus (boophilus) microplus (acari: ixodidae). | the present research aimed to study the chemical composition and acaricidal activity of citrus limonum and piper nigrum essential oils against the cattle tick rhipicephalus microplus. gc-ms analysis of c. limonum essential oil showed limonene (50.3%), β-pinene (14.4%), and γ-terpinene (11.7%) as the major components; p. nigrum oil was mainly composed of β-caryophyllene (26.2%), σ-ocymene (5.8%), and α-pinene (5.5%). acaricide activity was evaluated at concentrations of 2.5, 5.0, and 10.0% (v/v) ... | 2017 | 29123924 |
| molecular identification of tick-borne pathogens infecting cattle in mymensingh district of bangladesh reveals emerging species of anaplasma and babesia. | tick-borne diseases are considered a major hindrance to the health and productive performance of cattle in bangladesh. to elucidate the epidemiology of tick-borne pathogens (tbps) in local cattle, a cross-sectional study was performed in the 12 subdistricts (upazilas) of mymensingh district in bangladesh. blood samples and ticks were collected from 384 clinically healthy cattle kept by 135 farmers from 96 randomly selected villages. dna extracted from the blood samples was subsequently screened ... | 2017 | 29119682 |
| rickettsia spp. among wild mammals and their respective ectoparasites in pantanal wetland, brazil. | the genus rickettsia comprises obligatory intracellular bacteria, well known to cause zoonotic diseases around the world. the present work aimed to investigate the occurrence of rickettsia spp. in wild animals, domestic dogs and their respective ectoparasites in southern pantanal region, central-western brazil, by molecular and serological techniques. between august 2013 and march 2015, serum, whole blood and/or spleen samples were collected from 31 coatis, 78 crab-eating foxes, seven ocelots, 4 ... | 2018 | 29111373 |
| experimental efficacy of a vaccine against rhipicephalus australis. | despite several decades of chemical use the cattle tick, rhipicephalus australis, still represents an important threat for breeders of tick susceptible cattle under tropical conditions. the intense use of acaricides has lead to the development of multi-resistant strains of ticks and alternative means of control need to be developed to maintain control. a vaccine against ticks, based on the boophilus microplus bm86 protein, is one of the promising alternative means of tick control. in this invest ... | 2017 | 29110171 |
| genotyping acaricide resistance profiles of rhipicephalus microplus tick populations from communal land areas of zimbabwe. | acaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. communal farmers of zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. as a strategy towards developing an effective acaricide resistance management system in zimbabwe, screening was done by genotyping rhipicephalus microplus tick populations using molecular markers associated with resistance to these chemicals. the frequency of t ... | 2018 | 29103950 |
| experimental infection of rickettsia parkeri in the rhipicephalus microplus tick. | this study aimed to evaluate, by means of artificial feeding, the interaction between a pathogenic rickettsia and the hard tick r. microplus. we used partially engorged females fed on calves free of rickettsia spp. group 1 (g1), containing 20 ticks, was fed bovine blood only. group 2 (g2), containing 20 ticks, was fed blood containing uninfected vero cells, and group 3 (g3), containing 40 ticks, was fed blood containing vero cells infected with rickettsia parkeri. biological parameters of the no ... | 2018 | 29102467 |
| efficacy of commercial synthetic pyrethroids and organophosphates associations used to control rhipicephalus (boophilus) microplus in southern brazil. | this study evaluated the acaricidal efficiency of synthetic pyrethroids (sp) associated with organophosphates (op) against rhipicephalus (boophilus) microplus populations in the state of rio grande do sul, southern brazil. ticks were collected in 54 cattle farms and tested using in vitro adult immersion test against four commercially available acaricide mixtures of sp and op. only one of four acaricides, comprising a mixture of cypermethrin, chlorpyrifos, and fenthion, had a mean efficiency high ... | 2017 | 29091122 |
| the novel isoxazoline ectoparasiticide lotilaner (credelio™): a non-competitive antagonist specific to invertebrates γ-aminobutyric acid-gated chloride channels (gabacls). | the isoxazolines are a novel class of parasiticides that are potent inhibitors of γ-aminobutyric acid (gaba)-gated chloride channels (gabacls) and, to a lesser extent, of inhibitory glutamate-gated chloride channels (glucls). lotilaner (credelio™), a novel representative of this chemical class, is currently evaluated for its excellent ectoparasiticide properties. | 2017 | 29089046 |
| effect of recombinant glutathione s-transferase as vaccine antigen against rhipicephalus appendiculatus and rhipicephalus sanguineus infestation. | the ticks rhipicephalus appendiculatus and rhipicephalus sanguineus are the main vectors of theileria parva and babesia spp. in cattle and dogs, respectively. due to their impact in veterinary care and industry, improved methods against r. appendiculatus and r. sanguineus parasitism are under development, including vaccines. we have previously demonstrated the induction of a cross-protective humoral response against rhipicephalus microplus following vaccination with recombinant glutathione s-tra ... | 2017 | 29056423 |
| contributions to the knowledge of hard ticks (acari: ixodidae) in colombia. | the known tick fauna of colombia includes 58 species (15 argasidae and 43 ixodidae). to add to the knowledge of the biology of ticks in colombia, hard ticks (ixodidae) were collected from domestic animals or vegetation during 2014-2016 in 10 of colombia's departments. ticks were identified to species through morphological examinations. taxonomic identification was confirmed for some specimens by molecular methods, including phylogenetic analyses inferred from three tick genes (cytochrome c oxida ... | 2018 | 29055642 |
| expression profile of rhipicephalus microplus vitellogenin receptor during oogenesis. | the vitellogenin receptor (vgr), which belongs to the low-density lipoprotein receptors (ldlr) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. in the present study, the full sequence of rhipicephalus microplus vgr (rmvgr) and the partial sequence of rhipicephalus appendiculatus vgr (ravgr) orf were determined and cloned. the rmvgr amino acid sequence contains the five highly conserved structural motifs characteristic of ldlr superfamily member ... | 2018 | 29054547 |
| movement patterns of nilgai antelope in south texas: implications for cattle fever tick management. | wildlife, both native and introduced, can harbor and spread diseases of importance to the livestock industry. describing movement patterns of such wildlife is essential to formulate effective disease management strategies. nilgai antelope (boselaphus tragocamelus) are a free-ranging, introduced ungulate in southern texas known to carry cattle fever ticks (cft, rhipicephalus (boophilus) microplus, r. (b.) annulatus). cft are the vector for the etiological agent of bovine babesiosis, a lethal dise ... | 2017 | 28992923 |
| presence of borrelia spp. dna in ticks, but absence of borrelia spp. and of leptospira spp. dna in blood of fever patients in madagascar. | the occurrence of tick-borne relapsing fever and leptospirosis in humans in madagascar remains unclear despite the presence of their potential vectors and reservoir hosts. we screened 255 amblyomma variegatum ticks and 148 rhipicephalus microplus ticks from zebu cattle in madagascar for borrelia-specific dna. borrelia spp. dna was detected in 21 amblyomma variegatum ticks and 2 rhipicephalus microplus ticks. one borrelia found in one rhipicephalus microplus showed close relationship to borrelia ... | 2018 | 28986249 |
| molecular detection and identification of rickettsia species in ticks (acari: ixodidae) collected from belize, central america. | little is known about tick-borne rickettsial pathogens in belize, central america. we tested ixodid ticks for the presence of rickettsia species in three of the six northern and western belizean districts. ticks were collected from domestic animals and tick drags over vegetation in 23 different villages in november 2014, february 2015, and may 2015. a total of 2,506 collected ticks were identified to the following species: dermacentor nitens neumann (46.69%), rhipicephalus sanguineus (latreille) ... | 2017 | 28981693 |
| in-vitro efficacy of a botanical acaricide and its active ingredients against larvae of susceptible and acaricide-resistant strains of rhipicephalus (boophilus) microplus canestrini (acari: ixodidae). | ticks and tick-borne diseases are a major constraint for a sustainable cattle industry in the tropical and subtropical regions of the world. the development of resistance to most of the commonly used acaricides has led to an assessment of herbal products for acaricidal activity as an eco-friendly tick control alternative. a botanical product, essentria® ic-3 insect concentrate containing rosemary oil (10%), geraniol (5%) and peppermint oil (2%), acts on target pests by blocking octopamine. essen ... | 2017 | 28978457 |
| evaluation of phytotherapy alternatives for controlling rhipicephalus (boophilus) microplus in vitro. | the objective of this study was to identify the main chemical components of the essential oil of cuminum cyminum l. (cumin) and of the fixed oils of bertholletia excelsa (brazil nut) and of helianthus annuus (sunflower seed). as well as testing the three oils and three different commercial synthetic acaricides against engorged females of rhipicephalus (boophilus) microplus in order to explore their acaricidal efficacy. six different concentrations of the oils (200, 100, 50, 25, 12.5 and 6.25 mg/ ... | 2017 | 28977244 |
| differential haematobia irritans infestation levels in beef cattle raised in silvopastoral and conventional pasture systems. | the use of silvopastoral systems (sps) can be a good alternative to reduce the environmental impacts of livestock breeding in brazil. one of the reasons for its scarce adoption is the lack of information on health and productivity of cattle raised under these conditions. the experiment reported here was designed to compare the infestation by external parasites - the cattle tick (rhipicephalus microplus), horn fly (haematobia irritans), and larvae of the botfly (dermatobia hominis) - in beef catt ... | 2017 | 28969788 |
| molecular biology of amitraz resistance in cattle ticks of the genus rhipicephalus. | amitraz is an important product for the control of cattle ticks around the world. in comparison with other products for the control of ticks, it is quite affordable and it has a rapid knock-down effect. it binds with and activates adrenergic neuro-receptors of animals and it inhibits the action of monoamine oxidases (mao). resistance to amitraz has been documented in rhipicephalus microplus, r. decoloratus and r. appendiculatus. four mechanisms of resistance have been proposed, each of which is ... | 2018 | 28930573 |
| snps and other polymorhisms associated with acaricide resistance in rhipicephalus microplus. | ixodicides resistance of ticks is one of the most important problems for the livestock industry in tropical and subtropical regions, mainly due to the increase in cases of multiple resistance in all families of the ixodicides used. molecular markers such as single nucleotide polymorphisms (snps) has been proposed to identify the resistance to ixodicides in rhipicephalus microplus. many studies have recently been conducted using snps and other types of molecular markers to determine if they are a ... | 2018 | 28930538 |
| advances in tick vaccinology in brazil: from gene expression to immunoprotection. | rhipicephalus (boophilus) microplus has substantial economic impact on the cattle breeding industry and, chemical control and tick resistance development are the major concern. there is a worldwide search for new options, and control using vaccines has been the main focus nowadays. studies performed in brazil found that bm86-based immunization of bovines reduced the infestation of r. (b.) microplus of vaccinated bovines by 45% to 60%. native boophilusmicroplus tripsin inhibitors (bmtis) with try ... | 2018 | 28930522 |
| exploitation of chemical, herbal and nanoformulated acaricides to control the cattle tick, rhipicephalus (boophilus) microplus - a review. | the tick rhipicephalus (boophilus) microplus is a key vector of bacterial and protozoan diseases causing heavy economic losses directly and indirectly in animal husbandry. in the past decades, the control of ticks faced some major issues, such as the rapid development of resistance in targeted vectors and non-target effects on human health and the environment, due to the employ of synthetic acaricides and repellents. eco-friendly pesticides for treating and controlling animal parasites such as t ... | 2017 | 28917299 |
| functional characterization of candidate antigens of hyalomma anatolicum and evaluation of its cross-protective efficacy against rhipicephalus microplus. | hyalomma anatolicum and rhipicephalus microplus seriously affect dairy animals and immunization of host is considered as a sustainable option for the management of the tick species. identification and validation of protective molecules are the major challenges in developing a cross-protective vaccine. the subolesin (sub), calreticulin (crt) and cathepsin l-like cysteine proteinase (cathl) genes of h. anatolicum were cloned, sequenced and analysed for sequence homology. both ha-sub and ha-crt gen ... | 2017 | 28911904 |
| comparative study of esterases in deltamethrin and diazinon resistant rhipicephalus microplus and hyalomma anatolicum ticks collected from the trans-gangetic plains of india. | a comparative analysis of esterases in susceptible and resistant ticks revealed six types of esterases (est-1b, est-2b, est-3b, est-4b, est-5b and est-6b) in rhipicephalus microplus and four types (est-1h, est-2h, est-3h, est-4h) in hyalomma anatolicum using α-naphthyl acetate substrate. inhibition studies with eserine sulfate, p-chloromercuribenzoate, copper sulphate and phenylmethylsulfonyl fluoride revealed a marked variation in band intensity between susceptible and resistant ticks, with the ... | 2017 | 28894981 |
| morphological and molecular identification of rhipicephalus (boophilus) microplus in nigeria, west africa: a threat to livestock health. | the cattle tick rhipicephalus (boophilus) microplus was first reported in west africa in ivory coast, in 2007. since then it has made an aggressive eastward advancement having been reported in four other west african countries: mali, burkina faso, togo and benin. we herein report the first molecular identification of this tick species in nigeria, west africa, and highlight the threat it poses to livestock health. a nation-wide tick survey was conducted in 12 out of 36 states across the various a ... | 2017 | 28887701 |
| molecular detection of pathogens in ticks infesting cattle in nampula province, mozambique. | ticks are ectoparasites that can act as vectors of a large number of pathogens in wild and domestic animals, pets, and occasionally humans. the global threat of emerging or re-emerging tick-borne diseases supports the need for research focused in the zoonotic transmission, especially in countries like mozambique where rural populations are in close contact with domestic animals. the present study aims to: (1) identify tick species infesting cattle from monapo and nacala porto, districts of nampu ... | 2017 | 28856544 |
| nanostructured cinnamon oil has the potential to control rhipicephalus microplus ticks on cattle. | the aim of this study was to evaluate the capacity of pure and nanostructured cinnamon oil to control the infestation and reproductive efficiency of rhipicephalus microplus on dairy cows. in vitro (stage i)-engorged female ticks were immersed in concentrations of 1.0, 5.0 and 10% of cinnamon oil on its pure form, and 0.5, 1.0, and 5.0% of the nanostructured form. 10% cinnamon oil (pure form) showed 100% efficacy, whereas concentrations of 1 and 5% were 62 and 97% efficacious, respectively. nanoc ... | 2017 | 28852887 |
| acaricidal activity of essential oil of syzygium aromaticum, hydrolate and eugenol formulated or free on larvae and engorged females of rhipicephalus microplus. | the cattle tick, rhipicephalus microplus (canestrini, 1888) (ixodida: ixodidae), is the most important ectoparasite in cattle-breeding areas and is responsible for severe economic losses. synthetic acaricides have been used to control this parasite. however, the need for safer products has stimulated the search for new acaricides, such as those to be obtained from medicinal plants. the essential oil of syzygium aromaticum (clove) has many biological properties and shows great potential for use i ... | 2017 | 28833280 |
| purification of biotinylated cell surface proteins from rhipicephalus microplus epithelial gut cells. | rhipicephalus microplus - the cattle tick - is the most significant ectoparasite in terms of economic impact on livestock as a vector of several pathogens. efforts have been dedicated to the cattle tick control to diminish its deleterious effects, with focus on the discovery of vaccine candidates, such as bm86, located on the surface of the tick gut epithelial cells. current research focuses upon the utilization of cdna and genomic libraries, to screen for other vaccine candidates. the isolation ... | 2017 | 28784975 |
| acaricidal, pediculicidal and larvicidal activity of synthesized zno nanoparticles using momordica charantia leaf extract against blood feeding parasites. | the aim of the present study was to evaluate the acaricidal, pediculicidal and larvicidal effect of synthesized zinc oxide nanoparticles (zno nps) using momordica charantia leaf extract against the larvae of rhipicephalus (boophilus) microplus, adult of pediculus humanus capitis, and the larvae of anopheles stephensi, culex quinquefasciatus. the zno nps were characterized by using uv, xrd, ftir and sem-edx. the sem image confirms that the synthesized nanoparticles were spherical in shape with a ... | 2017 | 28760358 |
| evaluation of three adjuvants with respect to both adverse effects and the efficacy of antibody production to the bm86 protein. | cattle tick infestations remain an important burden for farmers in tropical area like in new caledonia. with the development of acaricide resistance, tick vaccines should be an attractive alternative to control ticks but their efficacy needs to be improved. in this study three adjuvants were studied in an experimental tick vaccine with a bm86 protein to assess their performance in terms of antibody productions and adverse reactions following vaccinations. the water-in-oil adjuvant isa 61 vg led ... | 2017 | 28752480 |
| the first assessment of the stress inducible defense of leucaena leucocephala with acaricidal potential effect against rhipicephalus (boophilus) microplus (acari: ixodidae). | plants respond to wounding caused by mechanical stress or herbivory by synthesizing defense proteins. there are no studies reporting the action of induced plant proteins against ticks. the aim of this study was to investigate the effect of mechanically wounded leucaena leucocephala leaves against rhipicephalus (boophilus) microplus. initially, we carried out time course experiments to evaluate the impact of mechanical wounding on the protein content and the peroxidase, catalase and protease inhi ... | 2017 | 28746447 |
| molecular and maldi-tof identification of ticks and tick-associated bacteria in mali. | ticks are considered the second vector of human and animal diseases after mosquitoes. therefore, identification of ticks and associated pathogens is an important step in the management of these vectors. in recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been reported as a promising method for the identification of arthropods including ticks. the objective of this study was to improve the conditions for the preparation of tick samples ... | 2017 | 28742123 |
| characterization of a novel cystatin type 2 from rhipicephalus microplus midgut. | the rhipicephalus (boophilus) microplus is an exclusive bovine ectoparasite responsible for the transmission of pathogens that decrease meat, leather and milk productions. cattle vaccination is an alternative to control tick infestations, but the discovery of potential antigens is still a challenge for researchers. recently, our group performed a midgut transcriptome of engorged r. microplus tick, and out of 800 ests sequences one cystatin-coding sequence was identified and named rmcystatin-4. i ... | 2017 | 28735872 |
| strategic control of rhipicephalus (boophilus) microplus infestation on beef cattle grazed in panicum maximum grasses in a subtropical semi-arid region of argentina. | the aim of this work was to test the efficacy of strategic control methods of rhipicephalus microplus infestation on beef cattle grazed in panicum maximum grasses in northwestern argentina. also, an analysis to discern how the r. microplus population was distributed amongst cattle was also performed to determine if partial selective treatment or cull the small proportion of more heavily infested animals are feasible options to control this tick. the strategic scheme of treatments was designed to ... | 2017 | 28716199 |
| a study of ticks and tick-borne livestock pathogens in pakistan. | as obligate blood-feeding arthropods, ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors. livestock farming plays a vital role in the rural economy of pakistan, and tick infestation causes serious problems with it. however, research on tick species diversity and tick-borne pathogens has rarely been conducted in pakistan. in this study, a systematic investigation of the tick species infesting livestock in different ecological regions of pakistan was co ... | 2017 | 28650978 |
| cellular response in the tick feeding site in crossbred cattle artificially infested by rhipicephalus microplus. | ticks and tick-borne diseases are of global importance and can cause serious economic losses to cattle-raising businesses. however, only few attempts have been made to describe the relationships between various cellular immune components and tick counts of, and therefore resistance to, rhipicephalus microplus. the present study evaluated the late inflammatory cellular response and dermal dendritic cells at the attachment site in naturally presensitized bovines, artificially infested by r. microp ... | 2017 | 28597313 |
| mechanisms of acaricide resistance in the cattle tick rhipicephalus (boophilus) microplus in sri lanka. | high tolerance of ticks to acaricides is increasingly becoming a problem to cattle farmers. resistance status of rhipicephalus (boophilus) microplus (acari: ixodidae)from two cattle farms of sri lanka were determined against different concentrations of pyrethroid permethrin, organophosphate malathion, organochlorine ddt and carbamate propoxur using larval packet test (lpt) as recommended by food and agriculture organization (fao). mechanisms of acaricide resistance were studied by conducting bio ... | 2017 | 28595924 |
| rickettsia amblyommatis infecting ticks and exposure of domestic dogs to rickettsia spp. in an amazon-cerrado transition region of northeastern brazil. | this study was performed in maranhão state, a transition area two brazilian biomes, amazon and cerrado. during 2011-2013, 1,560 domestic dogs were sampled for collection of serum blood samples and ticks in eight counties (3 within the amazon and 5 within the cerrado). a total of 959 ticks were collected on 150 dogs (9.6%). rhipicephalus sanguineus sensu lato (s.l.) was the most abundant tick (68% of all collected specimens), followed by amblyomma cajennense sensu lato (s.l.) (12.9%), amblyomma p ... | 2017 | 28594882 |
| rhipicephalus microplus and dermacentor nitens (acari: ixodidae) coparasitize white-tailed deer on st. john, u.s. virgin islands. | ticks parasitizing introduced white-tailed deer, odocoileus virginianus (zimmermann), on st. john, u.s. virgin islands, were recorded during and after drought conditions. tick infestation prevalences were 22% at the start of the drought (july 2015), 66% at the height of the drought (march 2016), and 35% after the drought had ended (july 2016; n = 67 deer). samples of ticks from 22 tranquilized deer in july 2016 revealed the presence of two species, the southern cattle tick, rhipicephalus (boophi ... | 2017 | 28591859 |
| cattle fever tick, rhipicephalus (boophilus) microplus (acari: ixodidae): potential control on pastures by the application of urea fertilizer. | the southern cattle fever tick, rhipicephalus (boophilus) microplus, spends as much as 80-90% of its life cycle as a larva questing for a host. standard control methods are limited to on-host applications, leaving a need for methods directed at the pasture infesting stages. reports from brazil indicate that pasture fertilization can reduce tick numbers. granular urea was tested using standard pesticide efficacy methods in both the laboratory and field trials to determine if there was a significa ... | 2017 | 28579028 |
| gene-enriched draft genome of the cattle tick rhipicephalus microplus: assembly by the hybrid pacific biosciences/illumina approach enabled analysis of the highly repetitive genome. | the genome of the cattle tick rhipicephalus microplus, an ectoparasite with global distribution, is estimated to be 7.1gbp in length and consists of approximately 70% repetitive dna. we report the draft assembly of a tick genome that utilized a hybrid sequencing and assembly approach to capture the repetitive fractions of the genome. our hybrid approach produced an assembly consisting of 2.0gbp represented in 195,170 scaffolds with a n50 of 60,284bp. the rmi v2.0 assembly is 51.46% repetitive wi ... | 2017 | 28577881 |
| molecular quantitative assay for esterase-mediated organophosphate resistance in rhipicephalus microplus. | the use of pesticides is the main tool to control infestations of the cattle tick rhipicephalus microplus, and organophosphate (op) is one of the most used compounds for this purpose. carboxylesterases (ches) are targets for op pesticides in arthropods, and acetylcholinesterase 2 (ache2) and esterase 1 (est1) are metabolic enzymes involved in the xenobiotic detoxification process. the increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qpcr) ... | 2017 | 28566216 |
| the effectiveness of a fixed-dose combination pour-on formulation of 1.25% fipronil and 2.5% fluazuron against economically important ectoparasites and associated pharmacokinetics in cattle. | the present work consisted of eight studies to evaluate the ectoparasiticidal spectrum and determine the pharmacokinetic parameters of a pour-on combination of fipronil 1.25mg/kg+fluazuron 2.5mg/kg for cattle against rhipicephalus microplus, haematobia irritans and the larvae of dermatobia hominis and cochliomyia hominivorax. the analysis fipronil and fluazuron were performed by liquid chromatography using a mass detector for the detection and quantification of analytes (lc-ms/ms). additionally, ... | 2017 | 28527785 |
| acaricidal activity of havardia albicans and caesalpinia gaumeri methanolic leaf extracts on rhipicephalus microplus and its toxicity to laboratory animals. | the acaricidal activity of methanolic extracts from the leaves of havardia albicans (kunth britton and rose) and caesalpinia gaumeri (greenm) were tested on the larvae and adults of the cattle tick rhipicephalus microplus canestrini using the larval immersion test and the adult immersion test, respectively. the toxicity of these extracts was also evaluated on laboratory animals using toxicity bioassays at different concentrations: skin irritability (500 mg/ml), acute oral toxicity (5000 mg/kg), ... | 2017 | 28497304 |
| review of cattle ticks (acari, ixodida) in ivory coast and geographic distribution of rhipicephalus (boophilus) microplus, an emerging tick in west africa. | the exotic tick rhipicephalus (boophilus) microplus was discovered in ivory coast in 2007 and then gradually in other countries in west africa. it is known to induce significant losses in farming and to replace other species of the same genus. in order to contribute to improve health and productivity of cattle in ivory coast regarding the emergence of this dreaded tick, a study was conducted to determine the current geographic distribution of the tick r. (b.) microplus and review cattle ticks in ... | 2017 | 28497303 |
| complete coding genome sequence for mogiana tick virus, a jingmenvirus isolated from ticks in brazil. | mogiana tick virus (mgtv) is a segmented jingmenvirus isolated in 2011 from cattle ticks in brazil. here, we present a complete coding genome sequence for mgtv isolate mgtv/v4/11, including all four segments. mgtv is evolutionarily related to the jingmen tick virus isolates sy84 and rc27. | 2017 | 28473376 |
| geno- and phenotypic characteristics of a transfected babesia bovis 6-cys-e knockout clonal line. | babesia bovis is an intra-erythrocytic tick-transmitted apicomplexan protozoan parasite. it has a complex lifestyle including asexual replication in the mammalian host and sexual replication occurring in the midgut of host tick vector, typically, rhipicephalus microplus. previous evidence showed that certain b. bovis genes, including members of 6-cys gene family, are differentially expressed during tick and mammalian stages of the parasite's life cycle. moreover, the 6-cys e gene is differential ... | 2017 | 28464956 |
| characterization and establishment of a reference deltamethrin and cypermethrin resistant tick line (ivri-iv) of rhipicephalus (boophilus) microplus. | the problem of ticks and tick borne diseases is a global threat and growing reports of resistance to commonly used insecticides further aggravated the condition and demands for country specific resistance monitoring tools and possible solutions of the problem. establishment of standard reference is prerequisite for development of monitoring tools. for studying possible role of different mechanisms involved in development of resistance in rhipicephalus (boophilus) microplus population and to deve ... | 2017 | 28456306 |
| mining a differential sialotranscriptome of rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations. | ticks cause massive damage to livestock and vaccines are one sustainable substitute for the acaricides currently heavily used to control infestations. to guide antigen discovery for a vaccine that targets the gamut of parasitic strategies mediated by tick saliva and enables immunological memory, we exploited a transcriptome constructed from salivary glands from all stages of rhipicephalus microplus ticks feeding on genetically tick-resistant and susceptible bovines. | 2017 | 28446245 |
| activity of carboxylesterases, glutathione-s-transferase and monooxygenase on rhipicephalus microplus exposed to fluazuron. | the objective of this study was to assess the effect of the exposure to fluazuron on the activity of common pesticide detoxification enzyme groups in the cattle tick (rhipicephalus microplus). engorged females of a susceptible strain (poa) and a resistant strain (jaguar) were exposed in vitro to fluazuron and their eggs and larvae were used to compare the activities of the general esterases, mixed-function oxidases (mfo) and glutathione-s-transferase (gst). the results showed significant elevati ... | 2017 | 28435029 |
| draft genome sequence of enterococcus casseliflavus pavet15 obtained from the oviduct infection of the cattle tick (rhipicephalus microplus) in jiutepec, morelos, mexico. | enterococcus spp. are gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. we report here the draft genome sequence of enterococcus casseliflavus pavet15 containing 3,722,480 bp, with 80 contigs, an n50 of 179,476 bp, and 41.93% g+c content. | 2017 | 28428300 |
| spotted fever: epidemiology and vector-rickettsia-host relationship in rio de janeiro state. | the eco-epidemiological scenario of spotted fever (sf), a tick-borne disease that affects humans and other animals in several countries around the world, was analyzed in rio de janeiro (rj) state, brazil. during the last 34 years, 990 sf cases were reported in rj (the brazilian state with the highest population density), including 116 cases confirmed by serology (rifi) or pcr, among 42.39% of the municipalities with reported cases of sf. the epidemiologic dynamics of sf in rj state are very hete ... | 2017 | 28424664 |
| distribution of ticks infesting ruminants and risk factors associated with high tick prevalence in livestock farms in the semi-arid and arid agro-ecological zones of pakistan. | tick infestation is the major problem for animal health that causes substantial economic losses, particularly in tropical and subtropical countries. to better understand the spatial distribution of tick species and risk factors associated with tick prevalence in livestock in pakistan, ticks were counted and collected from 471 animals, including 179 cattle, 194 buffaloes, 80 goats and 18 sheep, on 108 livestock farms in nine districts, covering both semi-arid and arid agro-ecological zones. | 2017 | 28420420 |
| extensive diversity of rickettsiales bacteria in ticks from wuhan, china. | rickettsiales bacteria are important agents of (re)emerging infectious diseases, with ticks playing a key role in their evolution and transmission. we collected 1079 hard ticks belonging to five species (ixodes sinensis, rhipicephalus microplus, haemaphysalis flava, haemaphysalis hystricis and haemaphysalis longicornis) from cattle and goats in wuhan city, hubei province, china. the dominant tick species was h. longicornis (578, 53.57%), followed by r. microplus (354, 32.81%), h. hystricis (62, ... | 2017 | 28411028 |
| the transcription factor relish controls anaplasma marginale infection in the bovine tick rhipicephalus microplus. | rhipicephalus microplus is an important biological vector of anaplasma marginale, the etiological agent of bovine anaplasmosis. the knowledge of tick immune responses to control bacterial infections remains limited. in this study, we demonstrate that transcription factor relish from the imd signaling pathway has an important role in the control of a. marginale infection in ticks. we found that rna-mediated silencing of relish caused a significant increase in the number of a. marginale in the mid ... | 2017 | 28408334 |
| in vitro acaricidal activity of crescentia cujete l. fruit pulp against rhipicephalus microplus. | cattle tick (rhipicephalus microplus) is an important constraint on livestock production, particularly in tropical and subtropical areas. synthetic acaricides are the primary method of control this thick. resistance of r. microplus to synthetic acaricides has given rise to the need of new scientific investigations on alternative ways to control it. the objective of this work was to conduct a chemical investigation of crescentia cujete l. fruit pulp and to evaluate the acaricide effect on r. micr ... | 2017 | 28391450 |
| the current status of resistance to alpha-cypermethrin, ivermectin, and amitraz of the cattle tick (rhipicephalus microplus) in ecuador. | rhipicephalus microplus is widely distributed in tropical and subtropical areas of the world where livestock is a principal activity with great veterinary and economic importance. in ecuador, this hematophagous ectoparasite has been observed between 0 and 2600 masl. one of the main tick control measures is the use of acaricides, which have been indiscriminately used worldwide and in ecuador. in this country, no studies on acaricide resistance in rhipicephalus microplus have been published. the c ... | 2017 | 28388639 |
| chemical composition and acaricide activity of an essential oil from a rare chemotype of cinnamomum verum presl on rhipicephalus microplus (acari: ixodidae). | the essential oils (eos) from the leaves of species cinnamomum verum j. presl are used in the pharmaceutical industry for their numerous biological activities. currently, the main compound of c. verum eo is eugenol which has acaricidal activity; however, a rare chemotype with benzyl benzoate as the main component can be found. benzyl benzoate is recognized as an acaricide; however, studies of the c. verum eos benzyl benzoate chemotype on rhipicephalus microplus were not reported. the aim of this ... | 2017 | 28342670 |
| non-parasitic life cycle of the cattle tick rhipicephalus (boophilus) microplus in panicum maximum pastures in northern argentina. | the aim of this work was to study the non-parasitic phase of the rhipicephalus microplus life cycle in panicum maximum grasses from northern argentina, in order to provide ecological information for designing methods of tick control. four localities were chosen as replicates. the biological parameters measured were proportion of females ovipositing, the pre-oviposition period, the proportion of egg clusters hatching, the incubation period of eggs, larval longevity, and the total non-parasitic pe ... | 2017 | 28342429 |
| a new method for in vitro feeding of rhipicephalus australis (formerly rhipicephalus microplus) larvae: a valuable tool for tick vaccine development. | rhipicephalus microplus is a hard tick that has a major impact on cattle health in tropical and subtropical regions because it feeds on cattle and is implicated in the transmission of pathogens that cause diseases such as bovine anaplasmosis and babesiosis. presently, acaricides are used to control tick infestation but this is becoming increasingly less effective due to the emergence of tick strains that are resistant to one or more classes of acaricides. anti-tick vaccines are a promising alter ... | 2017 | 28335800 |
| nanoparticle-based delivery of anaplasma marginale membrane proteins; virb9-1 and virb10 produced in the pichia pastoris expression system. | bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite anaplasma marginale in tropical and subtropical areas of the world. while difficult to express, the proteins virb9-1 and virb10 are immunogenic components of the outer membrane type iv secretion system that have been identified as candidate antigens for vaccines targeting of a. marginale. soluble virb9-1 and virb10 were successfully expressed using pichia pastoris. when formulated ... | 2016 | 28335329 |
| an experimental investigation into the effects of flooding on the ixodid tick boophilus microplus (canestrini). | the effect of water immersion on the non-parasitic stages of boophilus microplus were investigated both under laboratory and field conditions. survival of engorged female ticks was influenced by their age and the temperature of the water. eggs and larvae were more resistant to submerison than were engorged females and their survival was increased at low temperatures and in water with high oxygen content. the persistence of this tick in areas prone to flooding was attributed to the survival of pa ... | 1971 | 28310970 |
| immune recognition of salivary proteins from the cattle tick rhipicephalus microplus differs according to the genotype of the bovine host. | males of the cattle tick rhipicephalus microplus produce salivary immunoglobulin-binding proteins and allotypic variations in igg are associated with tick loads in bovines. these findings indicate that antibody responses may be essential to control tick infestations. infestation loads with cattle ticks are heritable: some breeds carry high loads of reproductively successful ticks, in others, few ticks feed and they reproduce inefficiently. different patterns of humoral immunity against tick sali ... | 2017 | 28288696 |
| detection of amitraz and malathion resistance in field populations of rhipicephalus (boophilus) microplus (acari: ixodidae) in jammu region of india. | detection of resistance levels against amitraz and malathion in rhipicephalus (boophilus) microplus collected from four districts of jammu region (india) was carried out using the adult immersion test. the regression graphs of probit mortality of ticks plotted against log values of concentration of drugs were utilised for the determination of slope of mortality, lethal concentration for 50% (lc50), 95% (lc95) and resistance factor (rf). on the basis of the data generated on variables (mortality, ... | 2017 | 28251410 |
| in vitro evaluation of acaricidal activity of novel green silver nanoparticles against deltamethrin resistance rhipicephalus (boophilus) microplus. | an investigation was undertaken to study, for the first time, in vitro acaricidal activity of green silver nanoparticles on deltamethrin resistance rhipicephalus (boophilus) microplus. the compounds tested were neem coated silver nanoparticles (n-ag nps), deltamethrin neem coated silver nanoparticles (dn-ag nps), 2, 3 dehydrosalannol (2,3 dhs), 2, 3 dhs coated silver nanoparticles (2, 3-dhs-ag nps), quercetin dihydrate (qdh) and qdh coated silver nanoparticles (qdh-ag nps). also included in this ... | 2017 | 28246003 |
| bioinformatic prediction of g protein-coupled receptor encoding sequences from the transcriptome of the foreleg, including the haller's organ, of the cattle tick, rhipicephalus australis. | the cattle tick of australia, rhipicephalus australis, is a vector for microbial parasites that cause serious bovine diseases. the haller's organ, located in the tick's forelegs, is crucial for host detection and mating. to facilitate the development of new technologies for better control of this agricultural pest, we aimed to sequence and annotate the transcriptome of the r. australis forelegs and associated tissues, including the haller's organ. as g protein-coupled receptors (gpcrs) are an im ... | 2017 | 28231302 |
| immunogenic potential of rhipicephalus (boophilus) microplus aquaporin 1 against rhipicephalus sanguineus in domestic dogs. | this study evaluated a recombinant aquaporin 1 protein of rhipicephalus (boophilus) microplus (rmaqp1) as antigen in a vaccine against r. sanguineus. five dogs were immunized with rmaqp1 (10 µg) + adjuvant (montanide) (g1), and five were inoculated with adjuvant only (g2), three times. twenty-one days after the last immunization, animals of both groups were challenged with r. sanguineus larvae, nymphs and adults, and their biotic potential was compared. blood samples were collected before each i ... | 2017 | 28225856 |
| pyrethroid resistance in iranian field populations of rhipicephalus (boophilus) annulatus. | resistance to acaricides in ticks is becoming increasingly widespread throughout the world; therefore, tick control requires resistance monitoring for each tick species. the aims of this study were to monitor the susceptibility status of the cattle tick, rhipicephalus (boophilus) annulatus (acari: ixodidae), against pyrethroid acaricides from mazandaran province, northern iran, and where resistance was evident, and establish the possible underlying mechanisms. fully engorged adult r. (b.) annula ... | 2017 | 28187834 |
| molecular and structural characterization of novel cystatins from the taiga tick ixodes persulcatus. | cystatins are cysteine peptidase inhibitors that in ticks mediate processes such as blood feeding and digestion. the ixodid tick ixodes persulcatus is endemic to the eurasia, where it is the principal vector of lyme borreliosis. to date, no i. persulcatus cystatin has been characterized. in the present work, we describe three novel cystatins from i. persulcatus, named jpipcys2a, jpipcys2b and jpipcys2c. in addition, the potential of tick cystatins as cross-protective antigens was evaluated by va ... | 2017 | 28174118 |
| control of infestations by ixodes ricinus tick larvae in rabbits vaccinated with aquaporin recombinant antigens. | tick-borne diseases greatly impact human and animal health worldwide, and vaccines are an environmentally friendly alternative to acaricides for their control. recent results have suggested that aquaporin (aqp) water channels have a key function during tick feeding and development, and constitute good candidate antigens for the control of tick infestations. | 2017 | 28161419 |
| analysis of stage-specific protein expression during babesia bovis development within female rhipicephalus microplus. | arthropod-borne protozoan pathogens have a complex life cycle that includes asexual reproduction of haploid stages in mammalian hosts and the development of diploid stages in invertebrate hosts. the ability of pathogens to invade, survive, and replicate within distinct cell types is required to maintain their life cycle. in this study, we describe a comparative proteomic analysis of a cattle pathogen, babesia bovis, during its development within the mammalian and tick hosts with the goal of iden ... | 2017 | 28152313 |
| immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick rhipicephalus microplus. | ticks attach to and penetrate their hosts' skin and inactivate multiple components of host responses in order to acquire a blood meal. infestation loads with the cattle tick, rhipicephalus microplus, are heritable: some breeds carry high loads of reproductively successful ticks, whereas in others, few ticks feed and reproduce efficiently. | 2017 | 28143523 |
| further studies on south african plants: acaricidal activity of organic plant extracts against rhipicephalus (boophilus) microplus (acari: ixodidae). | the goal of our research is to develop a lower cost eco-friendly tick control method because acaricides that are commonly used to control ticks are often toxic, harmful to the environment or too expensive for resource-limited farmers. acetone and ethanol extracts were prepared and their acaricidal activities determined against the southern cattle tick, rhipicephalus (boophilus) microplus. a 1% solution of each of the plant extracts was prepared for efficacy testing using the adapted shaw larval ... | 2017 | 28115176 |
| parasite infection of public databases: a data mining approach to identify apicomplexan contaminations in animal genome and transcriptome assemblies. | contaminations from various exogenous sources are a common problem in next-generation sequencing. another possible source of contaminating dna are endogenous parasites. on the one hand, undiscovered contaminations of animal sequence assemblies may lead to erroneous interpretation of data; on the other hand, when identified, parasite-derived sequences may provide a valuable source of information. | 2017 | 28103801 |
| molecular detection of anaplasma spp. and ehrlichia spp. in ruminants from twelve provinces of china. | anaplasma spp. and ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. there is little information on anaplasmosis and ehrlichiosis in ruminants in china. 16s rrna fret-qpcrs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of china for anaplasma spp. and ehrlichia spp. positive samples were further analyzed with a standard pcr for the glta. anaplasm ... | 2016 | 28096822 |