Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| correction: methodological approach to the ex vivo expansion and detection of t. cruzi-specific t cells from chronic chagas disease patients. | [this corrects the article doi: 10.1371/journal.pone.0178380.]. | 2017 | 28859177 |
| synthesis of a model trisaccharide for studying the interplay between the anti α-gal antibody and the trans-sialidase reactions in trypanosoma cruzi. | trypanosoma cruzi, the etiologic agent of chagas disease, is covered by a dense glycocalix mainly composed by glycoproteins called mucins which are also the acceptors of sialic acid in a reaction catalyzed by a trans-sialidase (tcts). sialylation of trypomastigote mucins protects the parasite from lysis by the anti α-galp antibodies from serum. the tcts is essential for the infection process since t. cruzi is unable to biosynthesize sialic acid. the enzyme specifically transfers it from a termin ... | 2017 | 28858610 |
| alkaloid constituents of the amaryllidaceae plant amaryllis belladonna l. | the plant family amaryllidaceae is well-known for its unique alkaloid constituents, which exhibit a wide range of biological activities. its representative, amaryllis belladonna, has a geographical distribution covering mainly southern africa, where it has significant usage in the traditional medicine of the native people. in this study, a. belladonna samples collected in brazil were examined for alkaloid content. alkaloid profiles of a. belladonna bulbs were generated by a combination of chroma ... | 2017 | 28858260 |
| ecological and physiological thermal niches to understand distribution of chagas disease vectors in latin america. | in order to assess how triatomines (hemiptera, reduviidae), chagas disease vectors, are distributed through latin america, we analysed the relationship between the ecological niche and the limits of the physiological thermal niche in seven species of triatomines. we combined two methodological approaches: species distribution models, and physiological tolerances. first, we modelled the ecological niche and identified the most important abiotic factor for their distribution. then, thermal toleran ... | 2017 | 28857300 |
| anti-cardiac troponin antibodies in clinical human disease: a systematic review. | anti-cardiac troponin antibodies have been studied in different types of clinical diseases and in healthy populations. a systematic review of published data on anti-troponin antibodies was carried out (search performed on pubmed, isi web of knowledge and scopus databases). from title and abstract analysis, thirty-three articles were included that met the pre-specified criteria; after full-text analysis, nine articles were excluded. most studies assessed anti-troponin i antibodies. the prevalence ... | 2017 | 28856147 |
| the use of herbs against neglected diseases: evaluation of in vitro leishmanicidal and trypanocidal activity of stryphnodendron rotundifolium mart. | the evaluation of the leishmanicidal and trypanocidal activity of the hydroalcoholic extract of the bark of stryphnodendron rotundifolium mart. (ehcsr) was carried out to find an alternative treatment for parasitic diseases. ehcsr was prepared and used at four different concentrations (1000, 500, 250, 125 μg/ml) in in vitro assays for activity against leishmania promastigotes using the species leishmania brasiliensis and leishmania infantum and for trypanocidal activity using the epimastigotes o ... | 2017 | 28855804 |
| antiprotozoal activity of extracts of elaeodendron trichotomum (celastraceae). | chagas disease, amebiasis, giardiasis and trichomoniasis represent a serious health problem in latin america. the drugs employed to treat these illnesses produce important side effects and resistant strains have appeared. the present study was aimed to evaluate the antiprotozoal activity of leaves, stem bark and root bark of elaeodendron trichotomum, a celastraceus, that is used in mexico as an anti-infective in febrile-type diseases. | 2016 | 28852732 |
| quantitative proteome and phosphoproteome analyses highlight the adherent population during trypanosoma cruzi metacyclogenesis. | trypanosoma cruzi metacyclogenesis is a natural process that occurs inside the triatomine vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. the biochemical alterations necessary for the differentiation process have been widely studied with a focus on adhesion and nutritional stress. here, using a mass spectrometry approach, a large-scale phospho(proteome) study was performed with the aim of understanding the metacyclogenesis p ... | 2017 | 28852088 |
| development of conventional and real-time multiplex pcr-based assays for estimation of natural infection rates and trypanosoma cruzi load in triatomine vectors. | chagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. the diversity of vector species and their habitats are among the factors that hinder the control of the disease. control programs periodically monitor the prevalence of t. cruzi infection in insect bugs through microscopical observation of diluted feces. however, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all ... | 2017 | 28851417 |
| short-range responses of the kissing bug triatoma rubida (hemiptera: reduviidae) to carbon dioxide, moisture, and artificial light. | the hematophagous bug triatoma rubida is a species of kissing bug that has been marked as a potential vector for the transmission of chagas disease in the southern united states and northern mexico. however, information on the distribution of t. rubida in these areas is limited. vector monitoring is crucial to assess disease risk, so effective trapping systems are required. kissing bugs utilize extrinsic cues to guide host-seeking, aggregation, and dispersal behaviors. these cues have been recog ... | 2017 | 28850059 |
| new trypanosoma species, trypanosoma gennarii sp. nov., from south american marsupial in brazilian cerrado. | hundreds of trypanosome species have been described in all mammalian orders, on every continent, including with mixed infections. trypanosomes circulate in the form of sylvatic enzootic infections transmitted by blood-sucking insects that are associated with the host mammals. small wild mammals were caught in a fragment of cerrado terrain on an island in the hydroelectric reservoir of três marias, in the central region of the state of minas gerais, using pitfall and sherman traps with different ... | 2017 | 28847674 |
| evaluation of proline analogs as trypanocidal agents through the inhibition of a trypanosoma cruzi proline transporter. | trypanosoma cruzi, the etiological agent of chagas disease, uses proline as its main carbon source, essential for parasite growth and stage differentiation in epimastigotes and amastigotes. since proline is involved in many essential biological processes in t. cruzi, its transport and metabolism are interesting drug targets. | 2017 | 28844978 |
| tandem affinity purification of exosome and replication factor c complexes from the non-human infectious kinetoplastid parasite crithidia fasciculata. | kinetoplastid parasites are responsible for a range of diseases with significant global impact. trypanosoma brucei and trypanosoma cruzi cause human african trypanosomiasis and chagas disease, respectively, while various leishmania species are responsible for cutaneous, mucocutaneous and visceral leishmaniasis. understanding the biology of these organisms is key for effective diagnosis, prophylaxis and treatment. the insect parasite crithidia fasciculata offers a safe and low-cost alternative fo ... | 2017 | 28844893 |
| etiological classification of stroke in patients with chagas disease using toast, causative classification system toast, and ascod phenotyping. | cardioembolism is considered a major pathophysiological mechanism in patients with ischemic stroke (is) and chagas disease (cd). however, a previous study reported that other stroke subtypes are present in more than 40% of cd patients according to the toast classification. therefore, the aim of our study was to evaluate the etiologic classification of stroke in patients with cd using the causative classification system (ccs), the ascod, and the toast classifications in a prospective cohort of pa ... | 2017 | 28844546 |
| discovery and optimization of 5-amino-1,2,3-triazole-4-carboxamide series against trypanosoma cruzi. | chagas' disease, caused by the protozoan parasite trypanosoma cruzi, is the most common cause of cardiac-related deaths in endemic regions of latin america. there is an urgent need for new safer treatments because current standard therapeutic options, benznidazole and nifurtimox, have significant side effects and are only effective in the acute phase of the infection with limited efficacy in the chronic phase. phenotypic high content screening against the intracellular parasite in infected vero ... | 2017 | 28844141 |
| automatic counting of trypanosomatid amastigotes in infected human cells. | this article presents an automatic approach to counting amastigotes in human cells infected with chagas. the approach is divided into four steps: first, morphological pretreatment removes the complex image background; sets are then segmented by unsupervised classification; the infected cells are then preserved using a thresholding process; and, finally, they undergo morphological granulometric processing and are filtered by the average. an experimental protocol was employed to compare the amasti ... | 2017 | 28841460 |
| cardiac inflammation and ventricular tachycardia in chagas disease. | 2017 | 28840107 | |
| cardiomyocyte oxidants production may signal to t. cruzi intracellular development. | chagas disease, caused by the protozoan parasite trypanosoma cruzi, presents a variable clinical course, varying from asymptomatic to serious debilitating pathologies with cardiac, digestive or cardio-digestive impairment. previous studies using two clonal t. cruzi populations, col1.7g2 (t. cruzi i) and jg (t. cruzi ii) demonstrated that there was a differential tissue distribution of these parasites during infection in balb/c mice, with predominance of jg in the heart. to date little is known a ... | 2017 | 28832582 |
| hunting hidden parasites: trypanosoma cruzi. | 2017 | 28831983 | |
| biological factors that impinge on chagas disease drug development. | chagas disease is caused by infection with the insect-transmitted protozoan trypanosoma cruzi, and is the most important parasitic infection in latin america. the current drugs, benznidazole and nifurtimox, are characterized by limited efficacy and toxic side-effects, and treatment failures are frequently observed. the urgent need for new therapeutic approaches is being met by a combined effort from the academic and commercial sectors, together with major input from not-for-profit drug developme ... | 2017 | 28831944 |
| morphometric wings similarity among sylvatic and domestic populations of triatoma infestans (hemiptera: reduviidae) from the gran chaco region of paraguay. | despite sustained efforts for eliminating triatoma infestans, reinfestation still persists in large part of the endemic area of chagas disease from the gran chaco region. sylvatic t. infestans populations seem to threat success of control programs of domestic t. infestans. in this study, we analyze whether t. infestans collected after a community-wide spraying were survivors or were immigrants from elsewhere using geometric morphometric tools. we used 101 right wings of female t. infestans captu ... | 2017 | 28829725 |
| a multi-parametric analysis of trypanosoma cruzi infection: common pathophysiologic patterns beyond extreme heterogeneity of host responses. | the extreme genetic diversity of the protozoan trypanosoma cruzi has been proposed to be associated with the clinical outcomes of the disease it provokes: chagas disease (cd). to address this question, we analysed the similarities and differences in the cd pathophysiogenesis caused by different parasite strains. using syngeneic mice infected acutely or chronically with 6 distant parasite strains, we integrated simultaneously 66 parameters: parasite tropism (7 parameters), organ and immune respon ... | 2017 | 28827716 |
| trypanocidal activity of mastoparan from polybia paulista wasp venom by interaction with tcgapdh. | chagas disease, considered a neglected disease, is a parasitic infection caused by trypanosoma cruzi, which is endemic throughout the world. previously, the antimicrobial effect of mastoparan (mp) from polybia paulista wasp venom against bacteria was described. to continue the study, we report in this short communication the antimicrobial effect of mp against trypanosoma cruzi. mp inhibits all t. cruzi developmental forms through the inhibition of tcgapdh suggested by the molecular docking. in c ... | 2017 | 28826757 |
| astrocyte apoptosis and hiv replication are modulated in host cells coinfected with trypanosoma cruzi. | the protozoan trypanosoma cruzi is the etiological agent of chagas disease. in immunosuppressed individuals, as it occurs in the coinfection with human immunodeficiency virus (hiv), the central nervous system may be affected. in this regard, reactivation of chagas disease is severe and often lethal, and it accounts for meningoencephalitis. astrocytes play a crucial role in the environment maintenance of healthy neurons; however, they can host hiv and t. cruzi. in this report, human astrocytes we ... | 2017 | 28824880 |
| trypanosoma infection favors brucella elimination via il-12/ifnγ-dependent pathways. | this study develops an original co-infection model in mice using brucella melitensis, the most frequent cause of human brucellosis, and trypanosoma brucei, the agent of african trypanosomiasis. although the immunosuppressive effects of t. brucei in natural hosts and mice models are well established, we observed that the injection of t. brucei in mice chronically infected with b. melitensis induces a drastic reduction in the number of b. melitensis in the spleen, the main reservoir of the infecti ... | 2017 | 28824630 |
| mast cell coupling to the kallikrein-kinin system fuels intracardiac parasitism and worsens heart pathology in experimental chagas disease. | during the course of chagas disease, infectious forms of trypanosoma cruzi are occasionally liberated from parasitized heart cells. studies performed with tissue culture trypomastigotes (tcts, dm28c strain) demonstrated that these parasites evoke neutrophil/cxcr2-dependent microvascular leakage by activating innate sentinel cells via toll-like receptor 2 (tlr2). upon plasma extravasation, proteolytically derived kinins and c5a stimulate immunoprotective th1 responses via cross-talk between brady ... | 2017 | 28824610 |
| relation between acetylcholinesterase and na(+), k(+)-atpase activities with impaired memory of mice experimentally infected by trypanosoma cruzi. | chagas disease is caused by the protozoan parasite trypanosoma cruzi and causes severe cardiac and brain damage, leading to behavioral alterations in humans and animals. however, the mechanisms involved in memory impairment during t. cruzi infection remain unknown. it has long been recognized that the enzymatic activities of acetylcholinesterase (ache) and na(+), k(+)-atpase are linked with memory dysfunction during other trypanosomiasis. thus, the aim of this study was to evaluate the involveme ... | 2017 | 28823791 |
| a strategy for scaling up access to comprehensive care in adults with chagas disease in endemic countries: the bolivian chagas platform. | bolivia has the highest prevalence of chagas disease (cd) in the world (6.1%), with more than 607,186 people with trypanosoma cruzi infection, most of them adults. in bolivia cd has been declared a national priority. in 2009, the chagas national program (chnp) had neither a protocol nor a clear directive for diagnosis and treatment of adults. although programs had been implemented for congenital transmission and for acute cases, adults remained uncovered. moreover, health professionals were not ... | 2017 | 28820896 |
| accuracy of a rapid diagnostic test (cypress chagas quick test(®)) for the diagnosis of chronic chagas disease in a nonendemic area: a retrospective longitudinal study. | we analyzed the accuracy of chagas quick test(®), a rapid diagnostic test, for the diagnosis of chronic chagas disease through a retrospective study on a cohort of 669 patients consecutively examined at a single reference center in italy, during a 7-year period. we observed high concordance with serological reference standard but low accuracy for screening purposes (sensitivity/specificity: 82.8%/98.7%) at least in our nonendemic context. | 2017 | 28820710 |
| prevalence and seroprevalence of trypanosoma cruzi infection in a military population in texas. | recent biosurveillance findings at joint base san antonio (jbsa), a large military installation located in south-central texas, indicate the potential for vector-borne human chagas disease. a cross-sectional study was conducted to determine the prevalence and seroprevalence of trypanosoma cruzi infection in highest risk subpopulations on the installation, including students and instructors who work and sleep in triatomine-endemic field settings. real-time polymerase chain reaction, enzyme-linked ... | 2017 | 28820695 |
| lack of efficacy of liposomal amphotericin b against acute and chronic trypanosoma cruzi infection in mice. | acute and chronic infection with trypanosoma cruzi affects millions of people. the current therapeutic options are highly toxic and often not effective. liposomal amphotericin b (laboratory) has been demonstrated previously to have some activity in murine models. in our studies, higher dosages given multiple times were tested for activity against acute or chronic disease, exploring whether intermittent and brief regimens could be effective, as might then prove useful in human, particularly outpa ... | 2017 | 28820684 |
| wild populations of triatoma infestans: compilation of positive sites and comparison of their ecological niche with domestic population niche. | for several years, the wild populations of triatoma infestans, main vector of trypanosoma cruzi causing chagas disease, have been considered or suspected of being a source of reinfestation of villages. the number of sites reported for the presence of wild t. infestans, often close to human habitats, has greatly increased, but these data are scattered in several publications, and others obtained by our team in bolivia have not been published yet. | 2017 | 28818626 |
| structural characterization of acidic m17 leucine aminopeptidases from the tritryps and evaluation of their role in nutrient starvation in trypanosoma brucei. | leucine aminopeptidase (lap) is found in all kingdoms of life and catalyzes the metal-dependent hydrolysis of the n-terminal amino acid residue of peptide or amino acyl substrates. laps have been shown to participate in the n-terminal processing of certain proteins in mammalian cells and in homologous recombination and transcription regulation in bacteria, while in parasites, they are involved in host cell invasion and provision of essential amino acids for growth. the enzyme is essential for su ... | 2017 | 28815215 |
| increased body exposure to new anti-trypanosomal through nanoencapsulation. | lychnopholide, a lipophilic sesquiterpene lactone, is efficacious in mice at the acute and chronic phases of chagas disease. conventional poly-ε-caprolactone (pcl) and long-circulating poly(d,l-lactide)-block-polyethylene glycol (pla-peg) nanocapsules containing lychnopholide were developed and characterized. lychnopholide presented high association efficiency (>90%) with the nanocapsules. a new, fast and simple hplc-uv-based bioanalytical method was developed, validated in mouse plasma and appl ... | 2017 | 28814794 |
| first records of triatoma rubrofasciata (de geer, 1773) (hemiptera, reduviidae) in foshan, guangdong province, southern china. | triatomines, also known as kissing bugs, which are found throughout the world and especially in latin america, are well known natural vectors that transmit american trypanosomiasis, also called chagas disease. in china, the presence of two species of triatoma (triatoma rubrofasciata and t. sinica) was recorded in the past. due to the growing population and the increasing risk of the global spread of chagas disease, triatomines became a potential public health nuisance, and in 2016, we started mo ... | 2017 | 28807005 |
| correlation of 6-minute walk test with left ventricular function and quality of life in heart failure due to chagas disease. | to evaluate the correlation of the total distance walked during the six-minute walk test (6-mwt) with left ventricular function and quality of life in patients with chagas disease (chd) complicated by heart failure. | 2017 | 28805026 |
| growth arrest and morphological changes triggered by emodin on trypanosoma cruzi epimastigotes cultivated in axenic medium. | emodin is an anthraquinone obtained from rheum palmatum rootstocks. here we tested the cytotoxic effects of emodin on trypanosoma cruzi epimastigotes, as well as the morphological changes that were induced by this compound in the parasite. emodin was permeable and blocked in vitro cell division of t. cruzi epimastigotes in axenic medium, causing growth arrest in a dose-dependent but reversible manner. emodin-exposed epimastigotes underwent duplication of organelles, such as the nucleus, kinetopl ... | 2017 | 28803999 |
| synthesis and in vitro evaluation of ca(2+) channel blockers 1,4-dihydropyridines analogues against trypanosoma cruzi and leishmania amazonensis: sar analysis. | drugs containing the1,4-dihydropyridine (dhp) core have recently attracted attention concerning their antiparasitic effect against various species of leishmania and trypanosoma. this approach named drugs repositioning led to interesting results, which have prompted us to prepare 21 dhp's analogues. the 1,4-dhp scaffold was decorated with different function groups at tree points including the nitrogen atom (nh and n-phenyl), the aryl group attached to c-4 (various substituted aryl residues) and t ... | 2017 | 28801098 |
| the trypomastigote small surface antigen (tssa) regulates trypanosoma cruzi infectivity and differentiation. | tssa (trypomastigote small surface antigen) is an antigenic, adhesion molecule displayed on the surface of trypanosoma cruzi trypomastigotes. tssa displays substantial sequence identity to members of the tcmuc gene family, which code for the trypomastigote mucins (tgpi-mucins). in addition, tssa bears sequence polymorphisms among parasite strains; and two tssa variants expressed as recombinant molecules (termed tssa-cl and tssa-sy) were shown to exhibit contrasting features in their host cell bi ... | 2017 | 28800609 |
| structure-based approaches targeting parasite cysteine proteases. | cysteine proteases are essential hydrolytic enzymes present in the majority of organisms, including viruses and unicellular parasites. despite the high sequence identity displayed among these proteins, specific structural features across different species grant distinct functions to these biomolecules, frequently related to pathological conditions. consequently, their relevance as promising targets for potential specific inhibitors has been highlighted and occasionally validated in recent decade ... | 2017 | 28799498 |
| development of a pharmacophore for cruzain using oxadiazoles as virtual molecular probes: quantitative structure-activity relationship studies. | chagas's is a neglected tropical disease caused by the protozoan parasite trypanosoma cruzi. according to the world health organization, 7 million people are infected worldwide leading to 7000 deaths per year. drugs available, nifurtimox and benzimidazole, are limited due to low efficacy and high toxicity. as a validated target, cruzain represents a major front in drug discovery attempts for chagas disease. herein, we describe the development of 2d qsar ([formula: see text] = 0.81) and a 3d-qsar ... | 2017 | 28795372 |
| enveloped and non-enveloped viral-like particles in trypanosoma cruzi epimastigotes. | electron microscopy is routinely used to identify viral infections in protozoan parasites. these viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. most of them are classified within the non-segmented dsrna totiviridae family. we observed virus-like particles (vlps) through transmission electron microscopy in the cytoplasm of trypanosoma cruzi epimastigotes grown in cultures. clusters of electrodense enveloped vlps having a diameter of 48 nm were ... | 2017 | 28793017 |
| lead selection of antiparasitic compounds from a focused library of benzenesulfonyl derivatives of heterocycles. | a library of 89 synthetic benzenesulfonyl derivatives of heterocycles with drug-like properties was assayed for in vitro antiparasitic activity and the results were added to our previously reported derivatives for a comprehensive sar evaluation. four compounds showed an ic50 between 0.25 and 3μm against leishmania donovani and low cytotoxicity. compound g{16} (1-(2,3,5,6-tetramethylphenylsulfonyl)-2-methylindoline), was particularly interesting with an ic50 similar to the reference drug miltefos ... | 2017 | 28789893 |
| in vitro antileishmanial activity and iron superoxide dismutase inhibition of arylamine mannich base derivatives. | leishmaniasis is one of the world's most neglected diseases, and it has a worldwide prevalence of 12 million. there are no effective human vaccines for its prevention, and treatment is hampered by outdated drugs. therefore, research aiming at the development of new therapeutic tools to fight leishmaniasis remains a crucial goal today. with this purpose in mind, we present 20 arylaminoketone derivatives with a very interesting in vitro and in vivo efficacy against trypanosoma cruzi that have now ... | 2017 | 28789716 |
| widespread trypanosoma cruzi infection in government working dogs along the texas-mexico border: discordant serology, parasite genotyping and associated vectors. | chagas disease, caused by the vector-borne protozoan trypanosoma cruzi, is increasingly recognized in the southern u.s. government-owned working dogs along the texas-mexico border could be at heightened risk due to prolonged exposure outdoors in habitats with high densities of vectors. we quantified working dog exposure to t. cruzi, characterized parasite strains, and analyzed associated triatomine vectors along the texas-mexico border. | 2017 | 28787451 |
| clinical predictors of inducible sustained ventricular tachycardia during electrophysiologic study in patients with chronic chagas' heart disease. | clinical independent predictors of inducible sustained ventricular tachycardia (vt) during electrophysiologic study (eps) are not known in patients with chronic chagas' heart disease. the purpose of this investigation was to fill this gap. | 2015 | 28785714 |
| assessment of speckle tracking strain predictive value for myocardial fibrosis in subjects with chagas disease. | one of the most challenging issues of chronic chagas disease is to provide earlier detection of heart involvement. two-dimensional speckle tracking (2-d st) echocardiography, a new imaging modality with useful applications in several cardiac diseases, has been validated for subjects with myocardial infarction against cardiac magnetic resonance (cmr). here we hypothesize that the longitudinal global strain (lgs) has an incremental value to ejection fraction for predicting myocardial fibrosis in s ... | 2015 | 28785684 |
| chagas heart failure in patients from latin america. | physicians working in europe and the united states should suspect chagas heart failure in every patient coming from latin america with chronic heart failure. diagnosis should be confirmed by positive serology. right bundle branch block and left anterior fascicular block on 12-lead electrocardiogram, enlarged cardiac silhouette with no pulmonary congestion on chest x-ray and left ventricular apical aneurysm on echocardiography are the distinctive features of this condition. the clinical course is ... | 2016 | 28785459 |
| a voucher system to speed review could promote a new generation of insecticides to fight vector-borne diseases. | many in the scientific community are concerned about the potential increase in prevalence of insect-borne diseases such as chagas disease, chikungunya, dengue fever, malaria, and zika in the united states and around the world. beyond vaccines and drugs to prevent and treat these diseases, a comprehensive approach to fighting these diseases should include control of disease-carrying vectors, such as mosquitoes. vector-control methods, such as using insecticides to treat bed nets and spray the wal ... | 2017 | 28784739 |
| within-host temporal fluctuations of trypanosoma cruzi discrete typing units: the case of the wild reservoir rodent octodon degus. | chagas disease caused by trypanosoma cruzi is considered a major public health problem in america. after an acute phase the disease changes to a chronic phase with very low parasitemia. the parasite presents high genetic variability with seven discrete typing units (dtus): tci-tcvi and tc bat. the aim of this work is to evaluate fluctuation of parasitemia and t. cruzi dtus in naturally infected octodon degus. | 2017 | 28784152 |
| survey of pediatric infectious diseases society members about congenital chagas disease. | participants in a survey about congenital chagas disease, distributed electronically to pediatric infectious diseases society members, perceived having limited knowledge about congenital trypanosoma cruzi infection. most rarely or never consider the diagnosis in infants born to parents from latin america. improved awareness of congenital chagas disease and assessment of at-risk infants is needed. | 2017 | 28777208 |
| chagas disease: importance of rats as reservoir hosts of trypanosoma cruzi (chagas, 1909) in western mexico. | in mexico, the role of most species of mammals involved in the transmission cycle of trypanosoma cruzi chagas, 1909 is poorly known. it was carried out a study to investigate the importance of rats as reservoir of t. cruzi in western mexico, an area with important risk of transmission of t. cruzi to human. thirty-eight human dwellings were searched on two representative towns of western mexico along twelve months for collection of rats and triatomines. study rats (rattus norvegicus) berkenhout, ... | 2017 | 28774654 |
| galectin-3 knockdown impairs survival, migration, and immunomodulatory actions of mesenchymal stromal cells in a mouse model of chagas disease cardiomyopathy. | therapies based on transplantation of mesenchymal stromal cells (msc) hold promise for the management of inflammatory disorders. in chronic chagas disease cardiomyopathy (ccc), caused by chronic infection with trypanosoma cruzi, the exacerbated immune response plays a critical pathophysiological role and can be modulated by msc. here, we investigated the role of galectin-3 (gal-3), a beta-galactoside-binding lectin with several actions on immune responses and repair process, on the immunomodulat ... | 2017 | 28769980 |
| maldi-tof ms protein profiling for the rapid identification of chagas disease triatomine vectors and application to the triatomine fauna of french guiana. | triatomines are haematophagous insects involved in the transmission of trypanosoma cruzi, the aetiological agent of chagas disease. the vector competence of these arthropods can be highly variable, depending on the species. a precise identification is therefore crucial for the epidemiological surveillance of t. cruzi and the determination of at-risk human populations. to circumvent the difficulties of morphological identification and the lack of comprehensiveness of the genbank database, we here ... | 2017 | 28768559 |
| description of an oral chagas disease outbreak in venezuela, including a vertically transmitted case. | we describe the eleventh major outbreak of foodborne trypanosoma cruzi transmission in urban venezuela, including evidence for vertical transmission from the index case to her fetus. after confirming fetal death at 24 weeks of gestation, pregnancy interruption was performed. on direct examination of the amniotic fluid, trypomastigotes were detected. t. cruzi specific-polymerase chain reaction (pcr) also proved positive when examining autopsied fetal organs. finally, microscopic fetal heart exami ... | 2017 | 28767982 |
| bone marrow cell migration to the heart in a chimeric mouse model of acute chagasic disease. | chagas disease is a public health problem caused by infection with the protozoan trypanosoma cruzi. there is currently no effective therapy for chagas disease. although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare. | 2017 | 28767980 |
| inhibition of protein-protein interactions: new options for developing drugs against neglected tropical diseases. | wake up! sleeping sickness and chagas disease are neglected tropical diseases caused by trypanosome infections. small molecules that disrupt a crucial protein-protein interaction in the parasites offer a new approach to drug development for these diseases. | 2017 | 28766862 |
| lipidomics and anti-trypanosomatid chemotherapy. | trypanosomatids such as leishmania, trypanosoma brucei and trypanosoma cruzi belong to the order kinetoplastida and are the source of many significant human and animal diseases. current treatment is unsatisfactory and is compromised by the rising appearance of drug resistant parasites. novel and more effective chemotherapeutics are urgently needed to treat and prevent these devastating diseases, which relies on the identification of essential, parasite specific targets that are absent in the hos ... | 2017 | 28766182 |
| chagas disease: modulation of the inflammatory response by acetylcholinesterase in hematological cells and brain tissue. | chagas disease is an acute or chronic illness that causes severe inflammatory response, and consequently, it may activate the inflammatory cholinergic pathway, which is regulated by cholinesterases, including the acetylcholinesterase. this enzyme is responsible for the regulation of acetylcholine levels, an anti-inflammatory molecule linked to the inflammatory response during parasitic diseases. thus, the aim of this study was to investigate whether trypanosoma cruzi infection can alter the acti ... | 2017 | 28766165 |
| in silico molecular docking studies of new potential 4-phthalazinyl-hydrazones on selected trypanosoma cruzi and leishmania enzyme targets. | recently, a series of 4-phthalazinyl-hydrazones under its e-configuration have exhibited excellent in vitro antichagasic and antileishmanial profiles. preliminary assays on both parasites suggested that the most active derivatives act through oxidative and nitrosative stress mechanisms; however, their exact mode of actions as anti-trypanosomal and anti-leishmanial agents have not been completely elucidated. this motivated to perform a molecular docking study on essential trypanosomatid enzymes s ... | 2017 | 28763686 |
| [knowledge of vector-borne diseases (dengue, rickettsiosis and chagas disease) in physicians]. | the ecological conditions of yucatan made it a suitable region for the acquisition of vector-borne diseases such as dengue, rickettsiosis, and chagas disease. as the epidemiological burden of these diseases shows an alarming increase of severe cases, the early establishment of diagnosis and therapeutics by first-contact physicians is a critical step that is not being fulfilled due to several reasons, including poor knowledge. | 2017 | 28763070 |
| knockout of the gamma subunit of the ap-1 adaptor complex in the human parasite trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain. | the ap-1 adaptor complex assists clathrin-coated vesicle assembly in the trans-golgi network (tgn) of eukaryotic cells. however, the role of ap-1 in the protozoan trypanosoma cruzi-the chagas disease parasite-has not been addressed. here, we studied the function and localization of ap-1 in different t. cruzi life cycle forms, by generating a gene knockout of the large ap-1 subunit gamma adaptin (tcap1-γ), and raising a monoclonal antibody against tcap1-γ. co-localization with a golgi marker and ... | 2017 | 28759609 |
| from lemongrass to ivermectin: ethnomedical management of chagas disease in tropical bolivia. | chagas disease is a neglected tropical disease; the only viable drugs are outdated and produce frequent side effects, and the overwhelming majority of cases are undiagnosed and untreated. globally, people encounter numerous impediments to accessing biomedical treatment for chagas disease. however, little is known about how people with chagas disease manage their health outside the biomedical system. in this article, i discuss knowledge of ethnomedical treatments among marginalized patients in an ... | 2017 | 28759265 |
| endogenous c-terminal tagging by crispr/cas9 in trypanosoma cruzi. | to achieve the c-terminal tagging of endogenous proteins in t. cruzi we use the cas9/ptrex-n vector (lander et al., 2015) to insert a specific tag sequence (3xha or 3xc-myc) at the 3' end of a specific gene of interest (goi). chimeric sgrna targeting the 3' end of the goi is pcr-amplified and cloned into cas9/ptrex-n vector. then a dna donor molecule to induce dna repair by homologous recombination is amplified. this donor sequence contains the tag sequence and a marker for antibiotic resistance ... | 2017 | 28758140 |
| dynamized ethyl alcohol improves immune response and behavior in murine infection with trypanosoma cruzi. | to evaluate the effects of dynamized ethyl alcohol (ethylicum)6ch and 30ch in mice infected with t. cruzi. | 2017 | 28757362 |
| phosphorus protects cardiac tissue by modifying the immune response in rats infected by trypanosoma cruzi. | this study evaluates and correlates the number of myocarditis focuses and production of cytokines in rattus norvegicus (wistar lineage), experimentally infected with t. cruzi and treated with phosphorus. | 2017 | 28757361 |
| draft genome sequence of an aminoglycoside-resistant rmtd-2-producing enterobacter cloacae subsp. cloacae (st)395 in brazil. | enterobacter cloacae has recently emerged as an important agent of nosocomial infections, due to the diffusion of extended spectrum β-lactamases and carbapenemases in this species. in this context, a raise in the use of aminoglycosides for therapy was noticed, being followed by the accelerated development of resistance mechanisms. in this study, we report the draft genome sequence of a multidrug-resistant enterobacter cloacae subsp. cloacae strain (ec2), isolated from active surveillance culture ... | 2017 | 28757348 |
| a community-based screening program for chagas disease in the usa. | chagas disease (cd) affects over 300 000 people in the usa, many with limited access to healthcare. although early detection and treatment prevents life-threatening complications, <1% of people with cd receive diagnosis, and routine screening is virtually nonexistent in the usa. we describe a program that led to an increase in cd screening in the latin american-born population of los angeles. | 2017 | 28756912 |
| identification of structure-stabilizing interactions in enzymes: a novel mechanism to impact enzyme activity. | cruzain, a cysteine protease in the cathepsin family, is pivotal to the life-cycle of trypanosoma cruzi, the etiological agent in chagas disease. current inhibitors of cruzain suffer from drawbacks involving gastrointestinal and neurological side effects and as a result have spurred the search for alternative anti-trypanocidals. through sequence alignment studies and intra-residue interaction analysis of the pro-protein of cruzain (pro-cruzain), we have identified a host of non-active site resid ... | 2017 | 28756483 |
| heteroleptic oxidovanadium(iv) complexes of 2-hydroxynaphtylaldimine and polypyridyl ligands against trypanosoma cruzi and prostate cancer cells. | in latin america chagas disease is an endemic illness caused by the parasite trypanosoma cruzi (t. cruzi), killing more people than any other parasitic disease. current chemotherapies are old and inadequate, thus the development of efficient ones is urgently needed. vanadium-based complexes have been shown to be a promising approach both against parasitic diseases and cancer and this study aims to achieve significant advances in the pursue of effective compounds. heteroleptic vanadium complexes ... | 2017 | 28755573 |
| in silico, in vitro, x-ray crystallography, and integrated strategies for discovering spermidine synthase inhibitors for chagas disease. | chagas disease results from infection by trypanosoma cruzi and is a neglected tropical disease (ntd). although some treatment drugs are available, their use is associated with severe problems, including adverse effects and limited effectiveness during the chronic disease phase. to develop a novel anti-chagas drug, we virtually screened 4.8 million small molecules against spermidine synthase (spdsyn) as the target protein using our super computer "tsubame2.5" and conducted in vitro enzyme assays ... | 2017 | 28751689 |
| development, validation and application of a gc-ms method for the simultaneous detection and quantification of neutral lipid species in trypanosoma cruzi. | the development and validation of an analytical method for the simultaneous analysis of five neutral lipids in trypanosoma cruzi epimastigotes by gc-ms is presented in this study. the validated method meets all validation parameters for all components and the chromatographic conditions have been optimized during its development. this analytical method has demonstrated good selectivity, accuracy, within-day precision, recovery and linearity in each of the established ranges. in addition, detectio ... | 2017 | 28750236 |
| case report: successful lung transplantation from a donor seropositive for trypanosoma cruzi infection (chagas disease) to a seronegative recipient. | the increasing shortage of organs for transplantation has prompted transplant programs to investigate the use of extended criteria donors, such as those with transmissible infectious diseases. successful cases of organ transplantation (mostly kidney and liver) from trypanosoma cruzi seropositive donors to seronegative recipients have been reported. we present a case of lung transplantation from a donor serologically positive for chagas disease to a seronegative recipient, and provide a review of ... | 2017 | 28749767 |
| potential triazole-based molecules for the treatment of neglected diseases. | neglected diseases (nds) affect million of people, especially the poorest population around the world. several efforts to an effective treatment have proved insufficient at the moment. in this context, triazole derivatives have shown great relevance in medicinal chemistry due to a wide range of biological activities. this review aims to describe some of the most relevant and recent research focused on 1,2,3- and 1,2,4-triazole-based molecules targeting four expressive nds: chagas disease, malari ... | 2017 | 28748757 |
| dual role of monocyte-derived dendritic cells in trypanosoma cruzi infection. | pathogens can cause inflammation when inoculated into the skin. the vector-transmitted protozoan parasite trypanosoma cruzi induces poor cellular-infiltration and disseminates, causing high mortality in the experimental model. here, we characterized the inflammatory foci at the parasite inoculation site and secondary lymphoid organs using a murine model. while no macrophages and few neutrophils and monocytes (mo) were recruited into the skin, t. cruzi infection elicited the mobilization of ly6c( ... | 2017 | 28748529 |
| [nursing care for the newborns with congenital chagas' disease]. | in brazil, due to the urbanization of the rural diseases, the health services must be prepared to detect precociously the infections, control the propagation and attend these sick people. the authors did a bibliographic surveying about congenital chagas' disease, presented a study with a good evolution, tried to determine the nursing care to these newborns and the respectives objectives. | 1987 | 28746687 |
| peptides selected using phage library variants, effectively inhibit trypanosoma cruzi infection. | four peptide sequences characterized by high content of hydrophobic, charged, and polar amino acids were obtained from 23 clones of m13 phage. peptides p2 and p4 exhibited highest binding affinity for immobilized trypomastigotes. the inhibitory effects of peptides seemed to be due to blockade of certain epitopes on t. cruzi surface proteins responsible for interactions with the respective receptors of host cells. | 2017 | 28744646 |
| role of inactive and active trypanosoma cruzi trans-sialidases on t cell homing and secretion of inflammatory cytokines. | trans-sialidase from trypanosoma cruzi (tc-ts) belongs to a superfamily of proteins that may have enzymatic activity. while enzymatically active members (tc-ats) are able to transfer sialic acid from the host cell sialyl-glycoconjugates onto the parasite or to other molecules on the host cell surface, the inactive members (tc-its) are characterized by their lectinic properties. over the last 10 years, several papers demonstrated that, individually, tc-ats or tc-its is able to modulate several bi ... | 2017 | 28744279 |
| new class of antitrypanosomal agents based on imidazopyridines. | the present work describes the synthesis of 22 new imidazopyridine analogues arising from medicinal chemistry optimization at different sites on the molecule. seven and 12 compounds exhibited an in vitro ec50 ≤ 1 μm against trypanosoma cruzi (t. cruzi) and trypanosoma brucei (t. brucei) parasites, respectively. based on promising results of in vitro activity (ec50 < 100 nm), cytotoxicity, metabolic stability, protein binding, and pharmacokinetics (pk) properties, compound 20 was selected as a ca ... | 2017 | 28740614 |
| 3-hydroxykynurenine, a tryptophan metabolite generated during the infection, is active against trypanosoma cruzi. | the antiparasitic activity of 3-hydroxykynurenine (3-hk), one of the major tryptophan catabolites of the kynurenine pathway, against both trypanosoma cruzi evolutive forms that are important for human infection, trypomastigotes (tps) and amastigotes (am), possible targets in the parasite and the drug toxicity to mammalian cells have been investigated. 3-hk showed a potent activity against am with ic50 values in the micromolar concentration range, while the ic50 values to cause tps death was ∼600 ... | 2017 | 28740612 |
| therapeutic effects of sphingosine kinase inhibitor n,n-dimethylsphingosine (dms) in experimental chronic chagas disease cardiomyopathy. | chagas disease cardiomyopathy is a parasite-driven inflammatory disease to which there are no effective treatments. here we evaluated the therapeutic potential of n,n-dimethylsphingosine(dms), which blocks the production of sphingosine-1-phosphate(s1p), a mediator of cellular events during inflammatory responses, in a model of chronic chagas disease cardiomyopathy. dms-treated, trypanosoma cruzi-infected mice had a marked reduction of cardiac inflammation, fibrosis and galectin-3 expression when ... | 2017 | 28733584 |
| infectious diseases causing autonomic dysfunction. | to review infectious diseases that may cause autonomic dysfunction. | 2017 | 28730326 |
| comparative study and analytical verification of pcr methods for the diagnosis of congenital chagas disease. | congenital infection is currently the first cause of new cases of chagas disease in argentina and nonendemic areas worldwide. its diagnosis is of utmost importance to guarantee curative treatment. to improve such diagnosis, a transfer process of pcr tests to the national laboratory network has been initiated. we performed a comparative study of four pcr assays [two end-point pcr and two duplex real-time quantitative pcr (qpcr) procedures] to detect trypanosoma cruzi dna in blood samples. because ... | 2017 | 28727985 |
| small subunit ribosomal metabarcoding reveals extraordinary trypanosomatid diversity in brazilian bats. | bats are a highly successful, globally dispersed order of mammals that occupy a wide array of ecological niches. they are also intensely parasitized and implicated in multiple viral, bacterial and parasitic zoonoses. trypanosomes are thought to be especially abundant and diverse in bats. in this study, we used 18s ribosomal rna metabarcoding to probe bat trypanosome diversity in unprecedented detail. | 2017 | 28727769 |
| analytical sensitivity and specificity of a loop-mediated isothermal amplification (lamp) kit prototype for detection of trypanosoma cruzi dna in human blood samples. | this study aimed to assess analytical parameters of a prototype lamp kit that was designed for detection of trypanosoma cruzi dna in human blood. the prototype is based on the amplification of the highly repetitive satellite sequence of t.cruzi in microtubes containing dried reagents on the inside of the caps. the reaction is carried out at 65°c during 40 minutes. calcein allows direct detection of amplified products with the naked eye. inclusivity and selectivity were tested in purified dna fro ... | 2017 | 28727723 |
| study of infectious diseases in archaeological bone material - a dataset. | bones of human and ground sloth remains were analyzed for presence of trypanosoma cruzi by conventional pcr using primers tc, tc1 and tc2. sequence results amplified a fragment with the same product size as the primers (300 and 350pb). amplified pcr product was sequenced and analyzed on genbank, using blast. although these sequences did not match with these parasites they showed high amplification with species of bacteria. this article presents the methodology used and the alignment of the seque ... | 2017 | 28725674 |
| performance assessment of a trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. | diagnosing chronic chagas disease (cd) requires antibody--antigen detection methods, traditionally based on enzymatic assay techniques whose performance depend on the type and quality of antigen used. previously, 4 recombinant chimeric proteins from instituto de biologia molecular do paraná (ibmp-8.1 to -8.4) comprising immuno-dominant regions of diverse trypanosoma cruzi antigens showed excellent diagnostic performance in enzyme-linked immunosorbent assays. considering that next-generation plat ... | 2017 | 28724556 |
| systemic insecticide treatment of the canine reservoir of trypanosoma cruzi induces high levels of lethality in triatoma infestans, a principal vector of chagas disease. | despite large-scale reductions in chagas disease prevalence across central and south america, trypanosoma cruzi infection remains a considerable public health problem in the gran chaco region where vector-borne transmission persists. in these communities, peridomestic animals are major blood-meal sources for triatomines, and household presence of infected dogs increases t. cruzi transmission risk for humans. to address the pressing need for field-friendly, complementary methods to reduce triatom ... | 2017 | 28724448 |
| comparative repeatome analysis on triatoma infestans andean and non-andean lineages, main vector of chagas disease. | triatoma infestans is the most important chagas disease vector in south america. two main evolutionary lineages, named andean and non-andean, have been recognized by geographical distribution, phenetic and genetic characteristics. one of the main differences is the genomic size, varying over 30% in their haploid dna content. here we realize a genome wide analysis to compare the repetitive genome fraction (repeatome) between both lineages in order to identify the main repetitive dna changes occur ... | 2017 | 28723933 |
| evolution of anti-trypanosoma cruzi antibody production in patients with chronic chagas disease: correlation between antibody titers and development of cardiac disease severity. | chagas disease is one of the most important endemic infections in latin america affecting around 6-7 million people. about 30-50% of patients develop the cardiac form of the disease, which can lead to severe cardiac dysfunction and death. in this scenario, the identification of immunological markers of disease progression would be a valuable tool for early treatment and reduction of death rates. in this observational study, the production of anti-trypanosoma cruzi antibodies through a retrospect ... | 2017 | 28723905 |
| 2b-rad genotyping for population genomic studies of chagas disease vectors: rhodnius ecuadoriensis in ecuador. | rhodnius ecuadoriensis is the main triatomine vector of chagas disease, american trypanosomiasis, in southern ecuador and northern peru. genomic approaches and next generation sequencing technologies have become powerful tools for investigating population diversity and structure which is a key consideration for vector control. here we assess the effectiveness of three different 2b restriction site-associated dna (2b-rad) genotyping strategies in r. ecuadoriensis to provide sufficient genomic res ... | 2017 | 28723901 |
| silencing of two vitellogenin genes inhibits oviposition in the chagas disease vector triatoma infestans (hemiptera: reduviidae). | rna interference caused by exogenous double-stranded rna (dsrna) is used to downregulate crucial genes to control insects. the reproductive success of all oviparous species depends on vitellogenin (vg) biosynthesis and its accumulation in the developing oocytes. adult females of triatoma infestans were independently injected with two vg dsrnas (vg1 dsrna or vg2 dsrna) or nuclease-free water (control) 24 hours before feeding, and a group of adult females not injected was also analyzed (control). ... | 2017 | 28722589 |
| study of the salivary glands in triatominae (hemiptera, reduviidae, triatominae): their color and application to the chagas disease vector evolution. | chagas disease is caused by trypanosoma cruzi and transmitted by feces of a triatomine that has the habit of defecating during blood feeding. the salivary glands of triatomines are important to hematophagy because their saliva is rich in anticoagulant and hemolytic proteins. the salivary glands of some rhodnius species analyzed are reddish due to the presence of nitrophorins (antihemostatic activity). the present study aimed to analyze the color pattern of the salivary glands of 67 triatomine sp ... | 2017 | 28722578 |
| prevention of congenital chagas disease by benznidazole treatment in reproductive-age women. an observational study. | since the decline in new cases of infection by insect/vector, congenital chagas disease has become more relevant in the transmission of chagas disease. treatment with benznidazole significantly reduces the parasitemia, which constitutes an important factor linked to vertical transmission. the objective of this study was to evaluate whether treatment with benznidazole previously administered to women of childbearing age can prevent or reduce the incidence of new cases of congenital chagas disease ... | 2017 | 28720492 |
| the immunomodulatory effects of the enalapril in combination with benznidazole during acute and chronic phases of the experimental infection with trypanosoma cruzi. | trypanosoma cruzi infection triggers a chronic inflammatory process responsible for the alterations in the extracellular matrix and functionality of the heart. the angiotensin converting enzyme (ace) inhibitors affects t. cruzi in vitro surveillance and modulates in vivo some inflammatory mediators. in this study, we investigated the treatment with an ace inhibitor (enalapril) and the benznidazole (bz) in a single and combination therapies (ct) in c57bl/6 mice infected with vl-10 strain of the t ... | 2017 | 28720491 |
| simplified membrane feeding of bloodsucking triatomines (hemiptera: reduviidae). | laboratory-reared hematophagous triatomines that are the main vectors of chagas disease are generally fed on small vertebrates or on blood-filled membrane devices. such devices allow a large-scale rearing of these vectors without sacrificing host animals but are almost always expensive, fragile, not easily purchased, and usually difficult to use because they need to be sterilized before and carefully cleaned after each feeding. we present here a simple device to feed triatomines that is composed ... | 2017 | 28720489 |
| high triatoma brasiliensis densities and trypanosoma cruzi prevalence in domestic and peridomestic habitats in the state of rio grande do norte, brazil: the source for chagas disease outbreaks? | abstracta total of 2,431 triatoma brasiliensis were collected from 39 populations of paraíba (pb) and rio grande do norte (rn) states, brazil. in pb, trypanosoma cruzi infection was not detected in either peridomestic or domestic vector populations. in contrast, in rn, t. brasiliensis was detected with high parasite prevalence in these ecotopes (30.7-40.0%). moreover, peridomicile insect population densities were more than double the average densities of all other settings evaluated (19.17 versu ... | 2017 | 28719275 |
| homology modeling of leishmania donovani enolase and its molecular interaction with novel inhibitors. | the treatment of indian tropical disease such as kala-azar is likely to be troublesome to the clinicians as ampb- and miltefosine-resistant leishmania donovani has been reported. the rationale behind designed a novel inhibitors of model of l. donovani enolase and performing a binding study with its inhibitors to gain details of the interaction between protein residues and ligand molecules. | 2017 | 28717332 |
| cholinesterase inhibition reduces arrhythmias in asymptomatic chagas disease. | parasympathetic dysfunction may play a role in the genesis of arrhythmias in chagas disease. | 2017 | 28715142 |
| health care importance of treponema pallidum, chagas' disease and human immunodeficiency virus 1 among amerindians of argentina: an observational study. | the objective of this study was to estimate the prevalence of treponema pallidum, trypanosoma cruzi and human immunodeficiency virus 1 (hiv-1) in five amerindian populations of argentina. a retrospective study was conducted among 857 amerindian populations (112 kollas, 298 mbyá-guaraníes, 79 sagua huarpes, 368 wichis) from 2007 to 2010. screening and confirmation of t. pallidum, t. cruzi and hiv-1 were performed. t. pallidum and t. cruzi infections were detected in all communities with an overal ... | 2017 | 28712508 |