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metagenomics analysis of microbial communities associated with a traditional rice wine starter culture (xaj-pitha) of assam, india.this is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving illumine-based whole genome shotgun (wgs) sequencing method. metagenomic dna was extracted from rice wine starter culture concocted by ahom community of assam and analyzed using a miseq(®) system. a total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. data obtained from the use of several taxonomic profiling tools were ...201628330225
how native yeasts may influence the chemical profile of the brazilian spirit, cachaça?cachaça is the typical brazilian spirit, obtained by distillation of fermented sugarcane must, whose production is closely related to regional heritage and geographical traits. in this work, we investigate the influence of different autochthonous yeast species, in comparison to other commercial strains, on the chemical profile of cachaça. fermentations were performed with four different wild species, a mixed starter culture with native yeasts, and two commercial strains. procedures were carried ...201728290322
nitrile-hydrolyzing enzyme from meyerozyma guilliermondii and its potential in biosynthesis of 3-hydroxypropionic acid.3-hydroxypropionic acid (3-hp) is an important platform chemical in organic synthesis. traditionally, 3-hp was produced by chemical methods and fermentation process. in this work, a novel enzymatic method was developed for green synthesis of 3-hp. a yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-hpn) as the sole nitrogen source. it was identified to be meyerozyma guilliermondii cgmcc12935 by sequencing of the 18s ri ...201728285455
lsp1, a responsive protein from meyerozyma guilliermondii, elicits defence response and improves glycyrrhizic acid biosynthesis in glycyrrhiza uralensis fisch adventitious roots.this research explored the effects of protein and polysaccharide in meyerozyma guilliermondii on active compounds in glycyrrhiza uralensis fisch adventitious roots. in this study, a responsive protein lsp1 was purified from the meyerozyma guilliermondii since the excellent induction. the contents of total flavonoids (3.46 mg · g(-1) ), glycyrrhizic acid (0.41 mg · g(-1) ), glycyrrhetinic acid (0.41 mg · g(-1) ), and polysaccharide (94.49 mg · g(-1) ) in adventitious root peaked at lsp1 group, wh ...201728105652
biocatalytic reduction of hmf to 2,5-bis(hydroxymethyl)furan by hmf-tolerant whole cells.catalytic upgrading of 5-hydroxymethylfurfural (hmf), an important biobased platform chemical for high-value products, is currently of great interest. in this work, a new highly hmftolerant yeast strain-meyerozyma guilliermondii sc1103 was isolated, and biocatalytic reduction of hmf to 2,5-bis(hydroxymethyl)furan (bhmf) using its resting cells was reported. cosubstrates exerted a significant effect on the catalytic activity and selectivity of microbial cells as well as their hmf-tolerant levels ...201727966286
engineering a wild-type diploid saccharomyces cerevisiae strain for second-generation bioethanol production.the cost-effective production of second-generation bioethanol, which is made from lignocellulosic materials, has to face the following two problems: co-fermenting xylose with glucose and enhancing the strain's tolerance to lignocellulosic inhibitors. based on our previous study, the wild-type diploid saccharomyces cerevisiae strain bsif with robustness and good xylose metabolism genetic background was used as a chassis for constructing efficient xylose-fermenting industrial strains. the performa ...201627942436
diversity of spoilage fungi associated with various french dairy products.yeasts and molds are responsible for dairy product spoilage, resulting in significant food waste and economic losses. yet, few studies have investigated the diversity of spoilage fungi encountered in dairy products. in the present study, 175 isolates corresponding to 105 from various spoiled dairy products and 70 originating from dairy production environments, were identified using sequencing of the its region, the partial β-tubulin, calmodulin and/or efα genes, and the d1-d2 domain of the 26s r ...201727794247
pineapple (ananas comosus l. merr.) wine production in angola: characterisation of volatile aroma compounds and yeast native flora.a pineapple vinification process was conducted through inoculated and spontaneous fermentation to develop a process suitable for a quality beverage during two successive vintages in huambo, angola. wines obtained with the conventional saccharomyces cerevisiae strain, were analysed by gas chromatography, and a total of 61 volatile constituents were detected in the volatile fraction and 18 as glycosidically bound aroma compounds. concentration levels of carbonyl and sulphur compounds were in agree ...201727783969
identification of uncommon candida species using commercial identification systems.recently, several studies have revealed that commercial microbial identification systems do not accurately identify the uncommon causative species of candidiasis, including candida famata, meyerozyma guilliermondii, and c. auris. we investigated the accuracy of species-level identification in a collection of clinical isolates previously identified as c. famata (n = 38), c. lusitaniae (n = 1 2), and m. guilliermondii (n = 5) by the vitek 2 system. all 55 isolates were re-analyzed by the phoenix s ...201627713213
assessment of the factors contributing to the growth or spoilage of meyerozyma guilliermondii in organic yogurt: comparison of methods for strain differentiation.in this work we analyze the spoiling potential of meyerozyma guilliermondii in yogurt. the analysis was based on contaminated samples sent to us by an industrial laboratory over two years. all the plain and fruit yogurt packages were heavily contaminated by yeasts, but only the last ones, containing fermentable sugars besides lactose, were spoiled by gas swelling. these strains were unable to grow and ferment lactose (as the type strain); they did grow on lactate plus galactose, fermented glucos ...201527682098
gene expression of glycyrrhizin acid and accumulation of endogenous signaling molecule in glycyrrhiza uralensis fisch adventitious roots after saccharomyces cerevisiae and meyerozyma guilliermondii applications.this study reports the best culture conditions for roots growth and accumulation of active components by optimizing the parameters. glycyrrhiza uralensis adventitious roots metabolites were significantly increased after adding saccharomyces cerevisiae and meyerozyma guilliermondii. the highest contents of polysaccharide, glycyrrhizic acid, glycyrrhetinic acid and total flavonoids were obtained in meyerozyma guilliermondii group, the content of glycyrrhizic acid was 5.3-fold higher than the contr ...201627644996
an additional meyerozyma guilliermondii imh3 gene confers mycophenolic acid resistance in fungal ctg clade species.the fungal ctg clade comprises a number of well-known yeasts that impact human health or with high biotechnological potential. to further extend the set of molecular tools dedicated to these microorganisms, the initial focus of this study was to develop a mycophenolic acid (mpa) resistance cassette. surprisingly, while we were carrying out preliminary susceptibility testing experiments in a set of yeast species, meyerozyma guilliermondii, although not being a mpa producer, was found to be primar ...201627620459
characterization of an ene-reductase from meyerozyma guilliermondii for asymmetric bioreduction of α,β-unsaturated compounds.to characterize a novel ene-reductase from meyerozyma guilliermondii and achieve the ene-reductase-mediated reduction of activated c=c bonds.201627193896
enhanced citric acid production by a yeast yarrowia lipolytica over-expressing a pyruvate carboxylase gene.in this study, after the expression of a pyruvate carboxylase gene (pyc) cloned from meyerozyma guilliermondii in a marine-derived yeast yarrowia lipolytica swj-1b, a transformant pg86 obtained had much higher pyc activity than y. lipolytica swj-1b. at the same time, the pyc gene expression and citric acid (ca) production by the transformant pg86 were also greatly enhanced. when glucose concentration in the medium was 60.0 g l(-1), ca concentration formed by the transformant pg86 was 34.02 g l(- ...201627100721
a strain of meyerozyma guilliermondii isolated from sugarcane juice is able to grow and ferment pentoses in synthetic and bagasse hydrolysate media.the search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. a yeast strain isolated from sugarcane juice and identified as meyerozyma guilliermondii was evaluated for the ability to grow and ferment pentoses in synthetic media and in sugarcane bagasse hydrolysate. the yeast grew in xylose, arabinose and glucose at the same rate at an initial medium ph of 5.5. a ...201627038950
isolation and characterization of phenol degrading yeasts from wastewater in the coking plant of zarand, kerman.phenol and phenolic compounds are environmental pollutants present in industrial wastewaters such as coal tar, oil refineries and petrochemical plants. phenol removal from industrial effluents is extremely important for the protection of environment. usually, phenol degradation is carried out by physicochemical methods that are costly and produce hazardous metabolites. recently, phenol biodegradation has been considered. yeasts are the most important phenol biodegraders. in this study, the pheno ...201626887222
yeast associated with the ambrosia beetle, platypus koryoensis, the pest of oak trees in korea.oak tree death caused by symbiosis of an ambrosia beetle, platypus koryoensis, and an ophiostomatoid filamentous fungus, raffaelea quercus-mongolicae, has been a nationwide problem in korea since 2004. in this study, we surveyed the yeast species associated with p. koryoensis to better understand the diversity of fungal associates of the beetle pest. in 2009, a total of 195 yeast isolates were sampled from larvae and adult beetles (female and male) of p. koryoensis in cheonan, goyang, and paju; ...201526839506
a novel production method for high-fructose glucose syrup from sucrose-containing biomass by a newly isolated strain of osmotolerant meyerozyma guilliermondii.one osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. this selected strain, named skenny, was identified as meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial d1/d2 large-subunit domains of the 26s ribosomal rna. sk-enny was utilized to produce high-fructose glucose syrup (hfgs) from sucrose-containing biomass. conversion rates to hfgs from 310-610 g/l of pure su ...201626718465
the microcyclic conidial stage of coniochaeta pulveracea and its effect on selected biological interactions.coniochaeta pulveracea is a dimorphic lignicolous fungus that has mostly been isolated from decaying wood. however, relatively little work was conducted on the conditions for the dimorphic switch or the biological interactions of the fungus in its yeast-like microcyclic growth phase. therefore, in this study, the microcyclic conidiation of c. pulveracea strains and representatives of the closely related species, coniochaeta boothii and coniochaeta subcorticalis, was studied under different envir ...201626658947
impact of meyerozyma guilliermondii isolated from chickens against eimeria sp. protozoan, an in vitro analysis.avian coccidiosis is a disease caused worldwide by several species of parasite eimeria that causes significant economic losses. this disease affects chickens development and production, that most of times is controlled with anticoccidial drugs. although efforts have been made to address this disease, they have been made to control eimeria sporozoites, although enteric stages are often vulnerable, however; the parasite oocyst remains a problem that must be controlled, as it has a resistant struct ...201526552648
development of an affordable typing method for meyerozyma guilliermondii using microsatellite markers.despite previously published methods, there is still a lack of rapid and affordable methods for genotyping the meyerozyma guilliermondii yeast species. the development of microsatellite markers is a useful genotyping method in several yeast species. using the tandem repeat finder software, a total of 19 microsatellite motifs (di-, tri-, and tetra- repetition) were found in silico in seven of the nine scaffolds published so far. primer pairs were designed for all of them, although only four were ...201626476570
isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source.few yeasts have shown the potential to efficiently utilize hemicellulosic hydrolyzate as the carbon source. in this study, microorganisms isolated from the manaus region in amazonas, brazil, were characterized based on their utilization of the pentoses, xylose, and arabinose. the yeasts that showed a potential to assimilate these sugars were selected for the better utilization of lignocellulosic biomass. two hundred and thirty seven colonies of unicellular microorganisms grown on hemicellulosic ...201526436401
new depsidones and isoindolinones from the mangrove endophytic fungus meyerozyma guilliermondii (hz-y2) isolated from the south china sea.three new depsidones, botryorhodines e-g (1-3), and two new isoindolinones, meyeroguillines a and b (7 and 9), along with five known compounds were isolated from an endophytic fungus meyerozyma guilliermondii, derived from the mangrove plant kandelia obovata. their structures were elucidated by 1d and 2d nmr spectroscopy and high resolution mass spectrometry (hreims). compounds 1-6 exhibited strong α-glucosidase inhibitory activity with ic50 values ranging from 2.1 to 13.3 μm. moreover, kinetic ...201526425177
data set for cloning and characterization of heterologous transporters in saccharomyces cerevisiae and identification of important amino acids for xylose utilization.the efficient uptake is important for the xylose utilization by saccharomyces cerevisiae. a heterogenous transporter mgt05196p was cloned from meyerozyma guilliermondii and expressed in saccharomyces cerevisiae[1]. this data article contains the transport characteristics of mgt05196p in s. cerevisiae. the fluorescence of fusion protein mgt05196p-gfp expressing strain was located on the cell surface demonstrated that the heterogenous transporter mgt05196p was targeted to the plasma membrane of s. ...201526217774
evaluation of microbial quality and yeast diversity in fresh-cut apple.the present work's aim was to study the microbial quality of minimally processed apples commercialized in portugal. sixty eight samples of fresh-cut apple were analyzed before their best-before date in 2011 and 2012 for aerobic mesophilic and psychrotrophic microorganisms, total coliforms, lactic-acid bacteria (lab), coagulase-positive staphylococci and fungi. the parameters of food safety studied were cronobacter sakazakii, salmonella spp. and listeria sp. samples were analyzed according to sta ...201526187843
characterization of virulence-related phenotypes in candida species of the cug clade.candida species cause a variety of mucosal and invasive infections and are, collectively, the most important human fungal pathogens in the developed world. the majority of these infections result from a few related species within the "cug clade," so named because they use a nonstandard translation for that codon. some members of the cug clade, such as candida albicans, present significant clinical problems, whereas others, such as candida (meyerozyma) guilliermondii, are uncommon in patients. th ...201526150417
assessment of endophytic yeast diversity in rice leaves by a culture-independent approach.endophytic microorganisms inhabit internal plant tissues in the host plant without causing any symptoms or negative effects. although the diversity of endophytes has been evaluated by both culture-dependent and culture-independent methods, less information is available on yeast communities. therefore, in this study a culture-independent method was used to examine endophytic yeasts associated with rice leaves based on the large subunit of ribosomal dna using a semi-nested pcr technique. sequence ...201526122889
potential spoilage yeasts in winery environments: characterization and proteomic analysis of trigonopsis cantarellii.wine microbiota is complex and includes a wide diversity of yeast species. few of them are able to survive under the restrictive conditions of dry red wines. in our study we detected and identified seven yeast species of the order saccharomycetales that can be considered potential spoilers of wines due to physiological traits such as acidogenic metabolism and off-odor generation: arthroascus schoenii, candida ishiwadae, meyerozyma guilliermondii, pichia holstii, pichia manshurica, trigonopsis ca ...201526119188
cloning and characterization of heterologous transporters in saccharomyces cerevisiae and identification of important amino acids for xylose utilization.efficient and specific transporters may enhance pentose uptake and metabolism by saccharomyces cerevisiae. eight heterologous sugar transporters were characterized in s. cerevisiae. the transporter mgt05196p from meyerozyma guilliermondii showed the highest xylose transport activity among them. several key amino acid residues of mgt05196p were suggested by structural and sequence analysis and characterized by site-directed mutagenesis. a conserved aromatic residue-rich motif (yffyy, position 332 ...201525944766
genetic diversity of medically important and emerging candida species causing invasive infection.genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis.201525887032
assessment of epiphytic yeast diversity in rice (oryza sativa) phyllosphere in thailand by a culture-independent approach.the epiphytic yeast diversity in rice phyllosphere in thailand was investigated by a culture-independent technique based on the rflp pattern and the sequence of the d1/d2 domain of the large subunit rrna gene. forty-four samples of rice leaf were collected randomly from six provinces. the dna was extracted from leaf washing samples and the d1/d2 domain was amplified using pcr technique. the pcr products were cloned and then screened by colony pcr. of total 1121 clones, 451 clones (40.2 %) reveal ...201525842038
phenotypic and molecular diversity of meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen candida guilliermondii, which causes about 2% of the human blood infections. this yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. the question if isolates from different sources are physiologically and genetically similar, or if the v ...201525791010
aromatic components produced by non-saccharomyces cerevisiae derived from natural fermentation of grape.aromatic components are important functional products during the wine fermentation process. in the current study, nine strains (y10, y5, y21, y2, y19, y16, y3, y13 and y4) of non-saccharomyces were isolated from cabernet sauvignon grape wine. aromatic components from cabernet sauvignon-fermented wine were determined the phylogenetic evolution status of different non-saccharomyces based on 26s rdna and d1/d2 sequence analysis and analysed by a gas chromatography-mass spectrometry analysis, and th ...201525677508
immune gene expression profile of penaeus monodon in response to marine yeast glucan application and white spot syndrome virus challenge.immunostimulant potential of eight marine yeast glucans (yg) from candida parapsilosis r20, hortaea werneckii r23, candida spencermartinsiae r28, candida haemulonii r63, candida oceani r89, debaryomyces fabryi r100, debaryomyces nepalensis r305 and meyerozyma guilliermondii r340 were tested against wssv challenge in penaeus monodon post larvae (pl). structural characterization of these marine yeast glucans by proton nuclear magnetic resonance (nmr) indicated structures containing (1-6)-branched ...201525555812
unpasteurised commercial boza as a source of microbial diversity.boza is a cereal-based fermented beverage widely consumed in many countries of the balkans. the aim of this study was to investigate the microbiota of three bulgarian boza samples through a combination of culture-dependent and -independent methods with the long-term objective of formulating a multi-strain starter culture specifically destined for the manufacture of new cereal-based drinks. the isolation campaign for lactic acid bacteria (lab) allowed the identification of lactobacillus parabuchn ...201525437059
saccharomyces cerevisiae and non-saccharomyces yeasts in grape varieties of the são francisco valley.the aims of this work was to characterise indigenous saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties cabernet sauvignon, grenache, tempranillo, sauvignon blanc and verdejo used in the são francisco river valley, northeastern brazil. in this study, 155 s. cerevisiae and 60 non-saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the d1/d2 domains of the large subunit of the rrna gene. among the non-saccharomyces species ...201425242923
enhanced expression of the codon-optimized exo-inulinase gene from the yeast meyerozyma guilliermondii in saccharomyces sp. w0 and bioethanol production from inulin.in the present study, after the exo-inulinase gene inu1 from meyerozyma guilliermondii was optimized according to the codon usage bias of saccharomyces cerevisiae, both the optimized gene inu1y and the native gene inu1 were ligated into the homologous integration expression vector pmirsc11 and expressed in saccharomyces sp. w0. it was determined that the inulinase activity of the recombinant yeast y13 with the optimized gene inu1y was 43.84 u/ml, which was obviously higher than that (31.39 u/ml) ...201425236803
uncommon opportunistic yeast bloodstream infections from qatar.eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ...201424934803
application of issr-pcr for rapid strain typing of debaryomyces hansenii isolated from dry-cured iberian ham.yeast populations of dry-cured iberian ham isolated from seven industries in the province of badajoz were characterized by issr-pcr using the (cag)4 primer and pcr-rflp of the its1-5.8s rrna-its2 fragment, and identified by dna sequencing. a total of 242 isolates were analyzed, indicating the primary species present was debaryomyces hansenii at 80.9% of the isolates followed by candida zeylanoides at 10.3% of the isolates. the remainders of isolates were identified as yamadazyma triangularis, sp ...201424929738
identification and characterisation of xylanolytic yeasts isolated from decaying wood and sugarcane bagasse in brazil.in this study, yeasts associated with lignocellulosic materials in brazil, including decaying wood and sugarcane bagasse, were isolated, and their ability to produce xylanolytic enzymes was investigated. a total of 358 yeast isolates were obtained, with 198 strains isolated from decaying wood and 160 strains isolated from decaying sugarcane bagasse samples. seventy-five isolates possessed xylanase activity in solid medium and were identified as belonging to nine species: candida intermedia, c. t ...201424748334
disrupting the methionine biosynthetic pathway in candida guilliermondii: characterization of the met2 gene as counter-selectable marker.candida guilliermondii (teleomorph meyerozyma guilliermondii) is an ascomycetous species belonging to the fungal ctg clade. this yeast remains actively studied as a result of its moderate clinical importance and most of all for its potential uses in biotechnology. the aim of the present study was to establish a convenient transformation system for c. guilliermondii by developing both a methionine auxotroph recipient strain and a functional met gene as selection marker. we first disrupted the met ...201424700391
reliable differentiation of meyerozyma guilliermondii from meyerozyma caribbica by internal transcribed spacer restriction fingerprinting.meyerozyma guilliermondii (anamorph candida guilliermondii) and meyerozyma caribbica (anamorph candida fermentati) are closely related species of the genetically heterogenous m. guilliermondii complex. conventional phenotypic methods frequently misidentify the species within this complex and also with other species of the saccharomycotina ctg clade. even the long-established sequencing of large subunit (lsu) rrna gene remains ambiguous. we also faced similar problem during identification of yeas ...201424575831
yeast species and genetic heterogeneity within debaryomyces hansenii along the ripening process of traditional ewes' and goats' cheeses.the yeasts present during the ripening process of ewes' and goats' cheeses produced in a small traditional dairy in mediterranean spain were isolated and identified. five hundred and thirty strains pertaining to eleven yeast species representing eight genera were identified using molecular methods. debaryomyces hansenii was the yeast species most frequently isolated in all cheeses. other yeast species commonly found in dairy products were present at the first maturing weeks. two yeast species tr ...201424290639
bioprospection of bacteria and yeasts from atlantic rainforest soil capable of growing in crude-glycerol residues.the increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. this could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. we explored an atlantic rainforest soil sample to search for crude glycerol-degrading microorganisms. microcosms of this soil were established containing minimal medium + 8% crude glycerol (w/w); the biological activity was measured by respirome ...201324222222
successful use of posaconazole to treat invasive cutaneous fungal infection in a liver transplant patient on sirolimus.fungi are an important and common cause of cutaneous infections affecting solid organ transplant recipients. these infections can represent a primary site of infection with the potential for dissemination, or a manifestation of metastatic infection. the high morbidity and mortality associated with these infections necessitates urgent therapy with antifungal drugs; however, the interaction between these drugs and immunosuppressive therapies can be a major limitation because of drug toxicity. a ca ...201223730320
species of candida as a component of the nasal microbiota of healthy horses.respiratory infections are a common problem among equines and occur with variable rates of morbidity and mortality. although some fungal species are considered primary agents of respiratory tract infections in several mammals, their relevance in respiratory diseases of equines is frequently neglected. in the present study, we performed an active search for candida spp. in the nasal cavity of horses. the presence of candida spp. was investigated through the use of nasal swabs that were streaked o ...201323651178
candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics.candida guilliermondii (teleomorph meyerozyma guilliermondii) is an ascomycetous species belonging to the saccharomycotina ctg clade which has been studied over the last 40 years due to its biotechnological interest, biological control potential and clinical importance. such a wide range of applications in various areas of fundamental and applied scientific research has progressively made c. guilliermondii an attractive model for exploring the potential of yeast metabolic engineering as well as ...201323616192
impact of pichia guilliermondii on sow, litter, and weaned pig immune performance.three groups of gestating gilts and sows (exp. 1; n = 98) were used to determine the effects of pichia guilliermondii (pg), a whole yeast product (citristim, adm alliance nutrition), on dam and litter immune parameters. at breeding, gilts and sows were allotted to 1 of 3 treatments maintained through lactation: sow control (sc) diet or sc supplemented with 0.1 (s1) or 0.2% (s2) pg. pigs from groups 1 (exp. 2) and 2 (exp. 3) were weaned (21 d) and allotted in a 3 (sc, s1, or s2) × 2 [nursery with ...201223365405
co-culturing of pichia guilliermondii enhanced volatile flavor compound formation by zygosaccharomyces rouxii in the model system of thai soy sauce fermentation.the roles of salt-tolerant yeasts such as zygosaccharomyces rouxii, candida versatilis, and candida etchellsii in the production of volatile flavor compounds (vfcs) in soy sauce fermentation have been well documented. however, the knowledge of vfc production by other salt-tolerant yeasts is still limited. in this work, the roles of z. rouxii and pichia guilliermondii strains in vfc production were investigated in moromi medium as a model system for soy sauce fermentation. inoculation of a single ...201223290236
cloning, characterization and heterelogous expression of the inu1 gene from cryptococcus aureus hya.the inu1 gene (accession number: jx073660) encoding exo-inulinase from cryptococcus aureus hya was cloned and characterized. the gene had an open reading frame (orf) of 1653 bp long encoding an inulinase. the coding region of the gene was not interrupted by any intron. it encoded 551 amino acid residues of a protein with a putative signal peptide of 23 amino acids and the calculated molecular mass of 59.5 kda. the protein sequence deduced from the inulinase structural gene contained the inulinas ...201223268348
antifungal activity of meyerozyma guilliermondii: identification of active compounds synthesized during dough fermentation and their effect on long-term storage of wheat bread.preliminarily, 146 strains of yeasts were screened for the antifungal activity toward the indicator penicillium roqueforti dppmaf1. the strain meyerozyma guilliermondii lcf1353 was selected and used for dough fermentation. the water/salt soluble extract of the dough was analyzed by hplc and gc/ms-spme. the synthesis of the extracellular cell wall-degrading enzyme β-1,3-glucanase and ethyl-acetate was shown. the effect on conidia germination mainly suggested a fungistatic activity. m. guilliermon ...201323200658
isolation, identification, and activity in vitro of killer yeasts against colletotrichum gloeosporioides isolated from tropical fruits.a total of 580 yeasts strains, isolated from ceara state of brasil, were evaluated for their ability to produce killer toxin. of these strains, 29 tested positive for the killer phenotype and were further evaluated for their ability to control colletotrichum gloeosporioides germination in vitro. all yeast strains that expressed the killer phenotype were characterized by sequencing the d1/d2 regions of the large subunit of the rrna gene. five yeast strains provided a significant reduction in myce ...201322915228
preparative separation of helvolic acid from the endophytic fungus pichia guilliermondii ppf9 by high-speed counter-current chromatography.high-speed counter-current chromatography (hsccc) was applied for preparative separation of helvolic acid from the crude extract of the endophytic fungus pichia guilliermondii ppf9, associated with the medicinal plant paris polyphylla var. yunnanensis for the first time. the two-phase solvent system consisted of n-hexane-ethyl acetate-methanol-water (4.5:4.5:5.0:5.0, v/v) appending with phosphoric acid (0.2%, v/v) was employed. the revolution speed of the separation column, flow rate of the mobi ...201122805802
molecular identification of pichia guilliermondii, debaryomyces hansenii and candida palmioleophila.traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of pichia guilliermondii, debaryomyces hansenii and candida palmioleophila. as result, these species are often misidentified. in this work, we established a reliable method for the identification/differentiation of these species. our assay was validated by dna sequencing of the polymorphic region used in a real-time pcr assay driven by species-speci ...201122481884
internal transcribed spacer as a target to assess yeast biodiversity in italian taleggio pdo cheese.three batches of soft smear-ripened taleggio pdo cheese were made in northern italy during the summertime 2010. a total of 129 isolates cultured from cheese surface were examined by using pcr-based methods and sequencing of both the its1 region and d1 and d2 domains of the 26s rrna gene. sequence analysis of isolates brought to the identification of 6 species: debaryomyces hansenii, kluyveromyces lactis, kluyveromyces marxianus, yarrowia lipolytica, pichia guilliermondii, and torulaspora delbrue ...201122417557
identification of yeast and acetic acid bacteria isolated from the fermentation and acetification of persimmon (diospyros kaki).persimmon (diospyros kaki) is a seasonal fruit with important health benefits. in this study, persimmon use in wine and condiment production was investigated using molecular methods to identify the yeast and acetic acid bacteria (aab) isolated from the alcoholic fermentation and acetification of the fruit. alcoholic fermentation was allowed to occur either spontaneously, or by inoculation with a commercial saccharomyces cerevisiae wine strain, while acetification was always spontaneous; all thes ...201222265289
scutular tinea of the scrotum by candida guilliermondii. 201222240280
species and susceptibility distribution of 1062 clinical yeast isolates to azoles, echinocandins, flucytosine and amphotericin b from a multi-centre study.descriptive values were determined for eight antifungal agents within the course of a multi-centre study encompassing 1062 german and austrian clinical yeast isolates. candida albicans (54%) was the predominant species isolated followed by candida glabrata (22%), candida parapsilosis (6%), candida tropicalis (5.7%), candida krusei (4.3%), as well as eleven further candidal and four non-candida yeast species. while 519 (48.9%) isolates were tested susceptible to all antifungals tested, no isolate ...201222233267
an endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from candida guilliermondii.abstract: the gene for a eukaryotic phenolic acid decarboxylase of candida guilliermondii was cloned, sequenced, and expressed in escherichia coli for the first time. the structural gene contained an open reading frame of 504 bp, corresponding to 168 amino acids with a calculated molecular mass of 19,828 da. the deduced amino sequence exhibited low similarity to those of functional phenolic acid decarboxylases previously reported from bacteria with 25-39% identity and to those of pad1 and fdc1 p ...201222217315
Optimization of ethanol, citric acid, and a-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii.The present study deals with submerged ethanol, citric acid, and a-amylase fermentation by Saccharomyces cerevisiae SDB, Aspergillus niger ANSS-B5, and Candida guilliermondii CGL-A10, using date wastes as the basal fermentation medium. The physical and chemical parameters influencing the production of these metabolites were optimized. As for the ethanol production, the optimum yield obtained was 136.00 ± 0.66 g/l under optimum conditions of an incubation period of 72 h, inoculum content of 4% (w ...201122193823
the relationship between candida species charge density and chitosan activity evaluated by ion-exchange chromatography.chitosan, a natural biopolymer presents antifungal activity that seems to be dependent on the interaction of its cationic amino groups and yeast cell surface. in this work we used ion-exchange chromatography to assess the surface charge density of candida species and subsequently to relate this with their sensitivity profile to chitosan. the ability of several strains from distinct candida species to interact with strong anionic and cationic exchangers was tested and the yeasts charge surface wa ...201122080045
occurrence and growth of yeasts in processed meat products - implications for potential spoilage.spoilage of meat products is in general attributed to bacteria but new processing and storage techniques inhibiting growth of bacteria may provide opportunities for yeasts to dominate the microflora and cause spoilage of the product. with the aim of obtaining a deeper understanding of the potential role of yeast in spoilage of five different processed meat products (bacon, ham, salami and two different liver patés), yeasts were isolated, enumerated and identified during processing, in the final ...200822063618
[fungal nail infections - an update : part 2 - from the causative agent to diagnosis - conventional and molecular procedures.]trichophyton (t.) rubrum is the most frequently isolated dermatophyte in onychomycosis, both in germany and worldwide. t. interdigitale (formerly t. mentagrophytes var. interdigitale) follows in second place. a further however rarely isolated dermatophyte in onychomycosis is epidermophyton floccosum. candida parapsilosis, candida guilliermondii, and candida albicans, followed by trichosporon spp. are the most important yeasts which are found in onychomycosis. the molds most often responsible inc ...201122037818
Epidemiology and antifungal susceptibility of bloodstream fungal isolates in pediatric patients: a spanish multicenter prospective survey.Data on fungemia epidemiology and antifungal susceptibility of isolates from children are scarce, leading frequently to pediatric empirical treatment based on available adult data. The present study was designed to update the epidemiological, mycological, and in vitro susceptibility data on fungal isolates from children with fungemia in Spain. All fungemia episodes were identified prospectively by blood culture over 13 months at 30 hospitals. Tests of susceptibility to amphotericin B, flucytosin ...201122012014
species of candida isolated from anatomically distinct sites in military personnel in cuiabá, mato grosso, brazil.some fungi are natural inhabitants of the human body but may result in disease when conditions are conducive to their development. yeast infections are common and often occur in the skin and mucous membranes; however emerging species have changed this epidemiological profile. the ability to colonize different anatomical sites has been associated with the pathogenicity of candida when environmental conditions are particularly favorable. in the case of hot, humid climates, the attrition suffered b ...201121987132
evaluation of luminex xtag fungal analyte-specific reagents for rapid identification of clinically relevant fungi.invasive fungal infections (ifi) remain a serious threat to immunocompromised hosts. current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with ifi. recently, luminex molecular diagnostics (toronto, canada) developed 23 analyte-specific reagents (asrs) for the detection of the most common clinically relevant fungi. this study's objective ...201121880976
[chromate-resistant mutants of yeast pichia guilliermondii: isolation and characterization]. 201121861366
fluorescent protein fusions in candida guilliermondii.candida guilliermondii is an emerging fungal agent of candidiasis often associated with oncology patients. this yeast also remains a promising biotechnological model for the industrial production of value-added metabolites. in the present study, we developed a recipient strain as well as a set of plasmids for construction of fluorescent protein (fp) fusions in this species. we demonstrated that c. guilliermondii phosphoglycerate kinase transcription-regulating sequences allow a constitutive expr ...201121807108
characterization of the skin fungal microbiota in patients with atopic dermatitis and healthy subjects.patients with atopic dermatitis (ad) are highly susceptible to viral, bacterial, and fungal skin infections because their skin is dry, which are involved in the barrier function of the skin. therefore, the skin microbiota of patients with ad is believed to be different from that of healthy individuals. in the present study, we compared the skin fungal microbiota of nine patients with mild, moderate, or severe ad and ten healthy subjects using an rrna clone library. fungal d1/d2 large subunit (ls ...201121699559
purification and properties of phenolic acid decarboxylase from candida guilliermondii.a heat-labile phenolic acid decarboxylase from candida guilliermondii (an anamorph of pichia guilliermondii) was purified to homogeneity by simple successive column chromatography within 3-ádays. the molecular mass was 20-ákda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 36-ákda by gel-filtration chromatography, suggesting that the purified enzyme is a homodimer. the optimal ph and temperature were approximately 6.0 and 25-¦c. characteristically, more than 50% of the optimal ...201121681484
influence of serum on in vitro susceptibility testing of echinocandins for candida parapsilosis and candida guilliermondii.echinocandins are antifungal drugs used for the treatment of invasive candidiasis and aspergillosis. they bind to serum proteins within a rate of 96 to >99%. the effect of serum on in vitro echinocandin susceptibility tests of certain candida and aspergillus species was reported. this study was performed to determine the effect of human serum on in vitro susceptibility testing of echinocandins for clinical isolates of candida parapsilosis and candida guilliermondii, the species which generally h ...201121668525
ctab, triton x-100 and freezing-thawing treatments of candida guilliermondii: effects on permeability and accessibility of the glucose-6-phosphate dehydrogenase, xylose reductase and xylitol dehydrogenase enzymes.cells of candida guilliermondii (atcc 201935) were permeabilised with surfactant treatment (ctab or triton x-100) or a freezing-thawing procedure. treatments were monitored by in situ activities of the key enzymes involved in xylose metabolism, that is, glucose-6-phosphate dehydrogenase (g6pd), xylose reductase (xr) and xylitol dehydrogenase (xd). the permeabilising ability of the surfactants was dependent on its concentration and incubation time. the optimum operation conditions for the permeab ...201121664992
use of lactobacillus plantarum and glucose to control the fermentation of "bella di cerignola" table olives, a traditional variety of apulian region (southern italy).the purpose of this study was to evaluate the use of lactobacillus plantarum, isolated from table olives "bella di cerignola," a traditional variety of apulian region (southern italy), as a starter for this kind of food. we focused on the interaction of the starter with the natural occurring microflora, the quantitative/qualitative composition of yeast population, the decrease of ph, and the content of organic acids. after a preliminary characterization, 3 strains of lb. plantarum, selected for ...201021535552
drug-resistant cassettes for the efficient transformation of candida guilliermondii wild-type strains.candida guilliermondii is an opportunistic emerging fungal agent of candidiasis often associated with oncology patients. this yeast also remains an interesting biotechnological model for the industrial production of value-added metabolites. the recent whole-genome sequencing of the c. guilliermondii atcc 6260 reference strain provides an interesting resource for elucidating new molecular events supporting pathogenicity, antifungal resistance and for exploring the potential of yeast metabolic eng ...201121489135
bio-synthesis of gold and silver nanoparticles from candida guilliermondii and their antimicrobial effect against pathogenic bacteria.in the present study we investigated the extra cellular synthesis of gold and silver nanoparticles by using the yeast candida guilliermondii. the formation of noble metal nanoparticles was monitored by the uv-visible spectroscopy. as prepared gold and silver nanoparticles showed distinct surface plasmon peaks at 530 nm and 425 nm respectively. phase and morphology of the as synthesized materials were investigated by x-ray diffraction and electron microscopy techniques respectively. xrd patterns ...201121446434
direct conversion of inulin and extract of tubers of jerusalem artichoke into single cell oil by co-cultures of rhodotorula mucilaginosa tjy15a and immobilized inulinase-producing yeast cells.in this study, it was found that the immobilized inulinase-producing cells of pichia guilliermondii m-30 could produce 169.3 u/ml of inulinase activity while the free cells of the same yeast strain only produced 124.3 u/ml of inulinase activity within 48 h. when the immobilized inulinase-producing yeast cells were co-cultivated with the free cells of rhodotorula mucilaginosa tjy15a, r. mucilaginosa tjy15a could accumulate 53.2% oil from inulin in its cells and cell dry weight reached 12.2g/l. un ...201121411313
antifungal activity of plant extracts against candida species from oral lesions.seventy five patients with oral lesions attending the different departments of rajah muthiah medical college and hospital, annamalai university were screened for candida. forty six (61.3%) candida strains were isolated from the oral lesions. of the 46 candida strains, candida albicans accounted for 35 (76.08%), candida glabrata for 5 (10.86%), candida tropicalis and candida krusei for 2 (4.34%) each and candida parapsilosis and candida guilliermondii for one (2.17%) each. antifungal activity of ...200821369447
c- and n-catabolic utilization of tricarboxylic acid cycle-related amino acids by scheffersomyces stipitis and other yeasts.scheffersomyces stipitis and the closely related yeast candida shehatae assimilated the l-amino acids glutamate, aspartate and proline as both carbon and nitrogen sole sources. we also found this rarely investigated ability in ascomycetous species such as candida glabrata, c. reukaufii, c. utilis, debaryomyces hansenii, kluyveromyces lactis, k. marxianus, candida albicans, l. elongisporus, meyerozyma guilliermondii, c. maltosa, pichia capsulata and yarrowia lipolytica and in basidiomycetous spec ...201121360752
yeasts isolation from bovine mammary glands under different mastitis status in the mexican high plateu.the mastitis subclinical and clinical in cows caused by fungi has been increased specially by yeast of the genus candida.201121349344
identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast pichia guilliermondii using insertion mutagenesis.pichia guilliermondii is a representative of a group of so-called flavinogenic yeast species that overproduce riboflavin (vitamin b(2)) in response to iron limitation. using insertion mutagenesis, we isolated p. guilliermondii mutants overproducing riboflavin. analysis of nucleotide sequence of recombination sites revealed that insertion cassettes integrated into the genome disrupting p. guilliermondii genes similar to the vma1 gene of ashbya gossypii and saccharomyces cerevisiae and fes1 and fr ...201121261808
expression of the inulinase gene from the marine-derived pichia guilliermondii in saccharomyces sp. w0 and ethanol production from inulin.it has been confirmed that saccharomyces sp. w0 can produce high concentration of ethanol. in this study, the inu1 gene cloned from the marine-derived pichia guilliermondii was transformed into uracil mutant of saccharomyces sp. w0. the positive transformant inu-66 obtained could produce 34.2 u ml⁻¹ of extracellular inulinase within 72 h of cultivation. it was found that 15.2 u of inulinase activity per one gram of inulin was suitable for inulin hydrolysis and ethanol production by the transform ...201021255354
optimization of killer assays for yeast selection protocols.a new optimized semiquantitative yeast killer assay is reported for the first time. the killer activity of 36 yeast isolates belonging to three species, namely, metschnikowia pulcherrima, wickerhamomyces anomala and torulaspora delbrueckii, was tested with a view to potentially using these yeasts as biocontrol agents against the wine spoilage species pichia guilliermondii and pichia membranifaciens. the effectiveness of the classical streak-based (qualitative method) and the new semiquantitative ...201021229201
molecular identification of closely related candida species using two ribosomal intergenic spacer fingerprinting methods.recent changes in the epidemiology of candidiasis highlighted an increase in non- candida albicans species emphasizing the need for reliable identification methods. molecular diagnostics in fungal infections may improve species characterization, particularly in cases of the closely related species in the candida complexes. we developed two pcr/restriction fragment length polymorphism assays, targeting either a part of the intergenic spacer 2 or the entire intergenic spacer (igs) of ribosomal dna ...201021227390
relative incidence of ascomycetous yeasts in arctic coastal environments.previous studies of fungi in polar environments have revealed a prevalence of basidiomycetous yeasts in soil and in subglacial environments of polythermal glaciers. ascomycetous yeasts have rarely been reported from extremely cold natural environments, even though they are known contaminants of frozen foods. using media with low water activity, we have isolated various yeast species from the subglacial ice of four glaciers from the coastal arctic environment of kongsfjorden, spitzbergen, includi ...201121221569
the epidemiology of candida species associated with vulvovaginal candidiasis in an iranian patient population.vulvovaginal candidiasis is a common infection among women worldwide. according to previous epidemiological studies, candida albicans is the most common species of candida. the prevalence of non-candida species, however, is increasing. identification of candida species among the population will not only help health professionals to choose suitable antifungal treatments, but also prevent development of drug resistance. the aim of this study was to identify, using chromogenic agar medium, the cand ...201021194828
development of a ura5 integrative cassette for gene disruption in the candida guilliermondii atcc 6260 strain.we designed an efficient transformation system for candida guilliermondii based on a ura5 atcc 6260 derived recipient strain and a ura5 recyclable selection marker. this "ura5 blaster" disruption system represents a powerful tool to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.201021182877
echinocandin antifungal drugs in fungal infections: a comparison.this review compares the pharmacology, spectrum of antifungal activity, pharmacokinetic and pharmacodynamic properties, safety and clinical efficacy of the three licensed echinocandins: caspofungin, micafungin and anidulafungin. echinocandins inhibit the synthesis of 1,3-β-d-glucan, an essential component of the fungal cell wall, and represent a valuable treatment option for fungal infections. the echinocandins exhibit potent in vitro and in vivo fungicidal activity against candida species, incl ...201121175238
effect of competition and adverse culture conditions on aflatoxin production by aspergillus flavus through successive generations.strains of aspergillus flavus often degenerate with serial transfers on culture media, resulting in morphological changes and loss of aflatoxin production. however, degeneration does not readily occur in nature as indicated by the wild-type morphological characters of newly isolated strains and the high percentage of aflatoxigenic a. flavus from soil and crops in some geographic regions. in this study, three aflatoxin-producing strains of a. flavus were serially transferred using conidia for 20 ...200221156547
identification of two species of yeast-like symbiotes in the brown planthopper, nilaparvata lugens.to determine the species of the yeast-like symbionts (yls) in the brown planthoppers (bph), nilaparvata lugens, yls were first isolated and purified by ultracentrifugation from the fat bodies of bph, and then 18s rdna and internal transcribed spacer (its)-5.8s rdna sequences of yls were amplified with the different general primers for fungi. the results showed that the two different 18s and its-5.8s rdna sequences of yls were obtained. one 2291-bp dna sequence, which contained 18s and its-5.8s r ...201021153730
[onychomycosis-causing yeasts in four mexican dermatology centers and their antifungal susceptibility to azolic compounds].yeasts represent the second cause of nail fungal infection in the world, and candida albicans and candida parapsilosis are the two most common species.201021147249
[posttraumatic primary cutaneous aspergillosis with candida guilliermondii infection in a healthy host].opportunistic fungal infections are usually seen in immunocompromised patients. while candida is the most prevalent agent in such infections, aspergillus is at the second order. primary cutaneous aspergillosis is most common in immunocompromised patients but can rarely be seen in healthy hosts as well. we report a case of posttraumatic primary cutaneous aspergillosis and candida guilliermondii coinfection in a 70-years-old healthy man. the patient had an ulcerous lesion which developed in the si ...201021063980
[investigation of antifungal activity of ononis spinosa l. ash used for the therapy of skin infections as folk remedies].traditional folk remedies used for centuries come up focus of interest in recent years, due to the trend of use of herb-derived natural products. in addition, increasing morbidity and mortality rates of opportunistic fungal infections and accelerating antifungal resistance rates of fungi lead to the use of alternative therapies with herb-derived preparations as novel antifungals. ononis spinosa l. (spiny restharrow), which is classified in leguminosae family, is one of the plants used in herbal ...201021063975
antimicrobial metabolites from the endophytic fungus pichia guilliermondii isolated from paris polyphylla var. yunnanensis.three steroids and one nordammarane triterpenoid were isolated for the first time from the endophytic fungus pichia guilliermondii ppf9 derived from the medicinal plant paris polyphylla var. yunnanensis. by means of physicochemical and spectrometric analysis, they were identified as ergosta-5,7,22-trienol (1), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), ergosta-7,22-dien-3β,5α,6β-triol (3), and helvolic acid (4). both micro-dilution-colorimetric and spore germination assays were employed to evalu ...201021060302
rapyd - rapid annotation platform for yeast data.lower eukaryotes of the kingdom fungi include a variety of biotechnologically important yeast species that are in the focus of genome research for more than a decade. due to the rapid progress in ultra-fast sequencing technologies, the amount of available yeast genome data increases steadily. thus, an efficient bioinformatics platform is required that covers genome assembly, eukaryotic gene prediction, genome annotation, comparative yeast genomics, and metabolic pathway reconstruction. here, we ...201021040748
what do we know about candida guilliermondii? a voyage throughout past and current literature about this emerging yeast.candida guilliermondii is an uncommon isolate throughout most of the world, the behaviour of which as an environmental fungus, a human saprophyte and an agent of serious infections has been emphasised over the years. notably, illnesses caused by this pathogen mostly involve compromised cancer hosts and commonly lead patients to unfavourable outcomes. it is of concern that the yeast may acquire or inherently express reduced in vitro sensitivity to all antifungal classes, although widespread resis ...201021039941
characterization of α-rhamnosidase activity from a patagonian pichia guilliermondii wine strain.the purpose of this study was to characterize the α-l-rhamnosidase of pichia guilliermondii npcc1053 indigenous wine strain from north-patagonian region.201020860771
distribution of dimorphic yeast species in commercial extra virgin olive oil.recent microbiological research has demonstrated the presence of a rich microflora mainly composed of yeasts in the suspended fraction of freshly produced olive oil. some of the yeasts are considered useful as they improve the organoleptic characteristics of the oil during preservation, whereas others are considered harmful as they can damage the quality of the oil through the hydrolysis of the triglycerides. however, some dimorphic species can also be found among the unwanted yeasts present in ...201020832682
inhibitory action of toxic compounds present in lignocellulosic hydrolysates on xylose to xylitol bioconversion by candida guilliermondii.the inhibitory action of acetic acid, ferulic acid, and syringaldehyde on metabolism of candida guilliermondii yeast during xylose to xylitol bioconversion was evaluated. assays were performed in buffered and nonbuffered semidefined medium containing xylose as main sugar (80.0 g/l), supplemented or not with acetic acid (0.8-2.6 g/l), ferulic acid (0.2-0.6 g/l), and/or syringaldehyde (0.3-0.8 g/l), according to a 2(3) full factorial design. since only individual effects of the variables were obse ...201120820863
fermentation of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing.this work aims to evaluate the fermentability of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing using candida guilliermondii fti 20037 yeast. the inoculum was obtained from yeast culture in a medium containing glucose as a carbon source supplemented with rice bran extract, cacl(2).2h(2)o and (nh(4))(2)so(4) in 50 ml erlenmeyer flasks, containing 20 ml of medium, initial 5.5 ph under agitation of an orbital shaker (200 rpm ...201020740373
isolation of clinically relevant fungal species from solid waste and environment of dental health services.this study was undertaken to detect, identify and determine antifungal susceptibility of yeast strains isolated from dental solid waste and to evaluate airborne fungi in the brazilian dental health care environment and in the waste storage room.201020723041
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