Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
---|
activity of the pterophyllins 2 and 4 against postharvest fruit pathogenic fungi. comparison with a synthetic analog and related intermediates. | the antifungal activity of pterophyllin 2, pterophyllin 4, a 5-desmethyl analog of the latter and some of their synthetic intermediates, against three postharvest phytopathogenic fungi, was evaluated. the target fungi were rhizopus stolonifer, botrytis cinerea and monilinia fructicola, which affect fruits worldwide, causing important economic losses. the tests were carried out with imazalil and carbendazim as positive controls. minimum inhibitory concentrations and minimum fungicidal concentrati ... | 2017 | 29288027 |
mixed cropping regimes promote the soil fungal community under zero tillage. | fungi of yield soils represent a significant portion of the microbial biomass and reflect sensitivity to changes in the ecosystem. our hypothesis was that crops included in cropping regimes under the zero tillage system modify the structure of the soil fungi community. conventional and molecular techniques provide complementary information for the analysis of diversity of fungal species and successful information to accept our hypothesis. the composition of the fungal community varied according ... | 2017 | 29270767 |
saprotrophic soil fungi to improve phosphorus solubilisation and release: in vitro abilities of several species. | modern agriculture is dependent on phosphate rock (pr), which is a nonrenewable resource. improvement of phosphorus (p) availability for crops in agricultural soils represents a key strategy to slow down the depletion of pr. the aim of this study was to identify potential p biofertilisers among saprotrophic fungal species. we tested 30 fungal strains belonging to 28 taxa (4 zygomycota and 24 ascomycota) and with different life strategies. the study showed that many saprotrophic fungi have the ab ... | 2018 | 29159452 |
carvacrol and eugenol effectively inhibit rhizopus stolonifer and control postharvest soft rot decay in peaches. | this study aimed to investigate the antifungal mechanism of carvacrol and eugenol to inhibit rhizopus stolonifer and the control of postharvest soft rot decay in peaches. | 2018 | 29044849 |
characterization of α-amylase from soursop (annona muricata linn.) fruits degraded by rhizopus stolonifer. | rhizopus stolonifer is a fungus and one of the most common species of the genus rhizopus. the organism has been a very important microbe used in the field of industrial microbiology. it has been used in the production of many hydrolytic and extracellular enzymes among which is the α-amylase. this enzyme has found various uses in the industry. fruit juices are important sources of nutrients and they contain several important therapeutic properties that may reduce the risk of various diseases. an ... | 2016 | 29023043 |
synthesis, characterization and antimicrobial activities of copper derivatives of nhc-ii complexes. | caffeine, 1, 3, 7-trimethylxanthine is one of the xanthine derivatives that are for the most part utilized as a part of solutions as diuretics. the cu (ii) complexes have been synthesized from the n-heterocyclic carbene ligands. | 2017 | 29022998 |
reduction of chemical and biological oxygen demands from oil wastes via oleaginous fungi: an attempt to convert food by products to essential fatty acids. | the production of waste pollutants has become a major problem for many food and oil industries. however, oil wastes can provide alternative substrates for industry, which could help to solve environmental pollution problems. furthermore, oil wastes can be used as substrates to produce unsaturated fatty acids, which are important for health. | 2015 | 28959287 |
growth modeling to control (in vitro) fusarium verticillioides and rhizopus stolonifer with thymol and carvacrol. | the aim of this study was to evaluate the antifungal activity (in vitro) of thymol and carvacrol alone or in mixtures against fusarium verticillioides and rhizopus stolonifer, and to obtain primary growth models. minimal inhibitory concentration (mic) was evaluated with fungal radial growth with thymol or carvacrol concentrations (0-1600mg/l). mixtures were evaluated using concentrations below mic values. radial growth curves were described by the modified gompertz equation. mic values of carvac ... | 2017 | 28947087 |
physiological effects and mode of action of zno nanoparticles against postharvest fungal contaminants. | increasing concerns continue to be expressed about health hazards and environmental pollution resulting from the use of conventional fungicides for postharvest disease control. nanoparticles represent an alternative solution for postharvest disease management. the objective of this work was to assess the physiological effects and the antifungal efficiency of zno nanoparticles (zno nps) against a number of fungal contaminants. the efficacy of zno nps was qualitatively and quantitatively assessed ... | 2017 | 28941694 |
self-induction system for cellulase production by cellobiose produced from glucose in rhizopus stolonifer. | cellulolytic fungi have evolved a sophisticated genetic regulatory network of cellulase synthesis to adapt to the natural environment. even in the absence of lignocellulose, it still secretes low levels of "constitutive" cellulase for standby application. however, the mechanisms of this constitutive expression remain incompletely understood. here we identified a cellobiose synthetase (cbs) from rhizopus stolonifer, which has the capacity to catalyse the synthesis of cellobiose from uridine dipho ... | 2017 | 28860637 |
antifungal activity of the pygidial gland secretion of laemostenus punctatus (coleoptera: carabidae) against cave-dwelling micromycetes. | the antifungal potential of the pygidial gland secretion of the troglophilic ground beetle laemostenus punctatus from a cave in southeastern serbia against cave-dwelling micromycetes, isolated from the same habitat, has been investigated. eleven collected samples were analyzed and 32 isolates of cave-dwelling fungi were documented. a total of 14 fungal species were identified as members of the genera aspergillus, penicillium, alternaria, cladosporium, rhizopus, trichoderma, arthrinium, aureobasi ... | 2017 | 28584964 |
assessing the anti-fungal efficiency of filters coated with zinc oxide nanoparticles. | air filters support fungal growth, leading to generation of conidia and volatile organic compounds, causing allergies, infections and food spoilage. filters that inhibit fungi are therefore necessary. zinc oxide (zno) nanoparticles have anti-fungal properties and therefore are good candidates for inhibiting growth. two concentrations (0.012 m and 0.12 m) were used to coat two types of filters (melt-blown and needle-punched) for three different periods (0.5, 5 and 50 min). rhizopus stolonifer and ... | 2017 | 28572995 |
isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria. | annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure. | 2017 | 28507410 |
reveromycins a and b from streptomyces sp. 3-10: antifungal activity against plant pathogenic fungi in vitro and in a strawberry food model system. | this study was conducted to determine the antifungal activity of the metabolites from streptomyces sp. 3-10, and to purify and identify the metabolites. meanwhile, the taxonomic status of strain 3-10 was re-evaluated. the cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. the resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and oomycetes, accounting for 92% of the total 24 tested species, sug ... | 2017 | 28421050 |
essential oil composition and antifungal activity of melissa officinalis originating from north-est morocco, against postharvest phytopathogenic fungi in apples. | to investigate biological control methods against post-harvest phytopathogenic fungi in apples, tests on the antifungal activity of essential oil of melissa officinalis were carried out. the essential oil, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (gc-ms). analysis of the essential oil was able to detect 88.7% of the components. the main components are p-mentha-1,2,3-triol (13.1%), p-menth-3-en-8-ol (8.8%), pulegone (8.8%), piperitynone oxid ... | 2017 | 28389346 |
characterization of plasma membrane fraction from filamentous fungus rhizopus nigricans. | in the filamentous fungus rhizopus nigricans a steroid hydroxylating multienzyme system is inducible by progesterone and by several other steroids. the biological signal carried by progesterone might be mediated by receptors, located either in the plasma membrane or inside the cell. to elucidate the first possibility, plasma membrane fraction was examined for the presence of progesterone receptors. the isolation of plasma membrane from fungal homogenate containing different other membranes is di ... | 2000 | 28176100 |
purification of cytochrome p450 from filamentous fungus rhyzopus nigricans. | it has been reported in our previous studies that steroid hydroxylation system of rhizopus nigricans involves cytochrome p450 and nadph-cytochrome p450 reductase in the electron transport system. both enzymes are membrane bound and are located in the microsomal preparations of progesterone induced fungal mycelia. in order to identify and characterize the cytochrome p450 component of fungal monoxygenase system, microsomal proteins from induced mycelia were subjected to high q anion exchange and m ... | 2000 | 28176091 |
comparison of two monooxygenase systems with cytochrome p450 in filamentous fungus rhizopus nigricans. | besides the progesterone inducible steroid hydroxylase which catalyses the transformation of progesterone to 11α-hydroxyprogesterone the presence of another monooxygenase system in filamentous fungus rhizopus nigricans (r. nigricans) - lanosterol demethylase was confirmed. lanosterol demethylase was not inducible with progesterone. after subcellular fractionation both monooxygenase systems were found in microsomal fraction of fungus r. nigricans. to find out how to differentiate between the hydr ... | 2000 | 28176089 |
isolation, partial length sequence and expression of steroid inducible hps 70 gene from rhizopus nigricans. | previous studies have shown that filamentous fungus rhizopus nigricans responds to addition of different steroids into growth medium with induction of hydroxylation system and that some steroids provoke stress response. the purpose of this study was to investigate whether those steroids provoke induction of hsp70 gene(s), well studied markers of stress response in different cells and organisms. the expression studies of fungal hsp70 gene(s) using northern blot analysis showed that fungal hsp70 m ... | 2000 | 28176085 |
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens. | filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear. | 2017 | 28100314 |
extracellular biosynthesis of silver nanoparticles using rhizopus stolonifer. | synthesis of silver nanoparticles (agnps) has become a necessary field of applied science. biological method for synthesis of agnps by rhizopus stolonifer aqueous mycelial extract was used. the agnps were identified by uv-visible spectrometry, x-ray diffraction (xrd), transmission electron microscopy (tem) and fourier transform infrared spectrometry (ft-ir). the presence of surface plasmon band around 420 nm indicates agnps formation. the characteristic of the agnps within the face-centered cubi ... | 2017 | 28053592 |
g-protein coupled progesterone receptors in the plasma membrane of fungus rhizopus nigricans. | we have demonstrated simultaneous existence of progesterone receptors and gtpase activity in the membranes prepared from the filamentous fungus rhizopus nigricans. the results obtained with pertussis toxin treated fungal mycelium suggest that these receptors do not couple to gi-go-proteins and play a role in the induction of steroid hydroxylating enzyme system by steroid substrates in the fungus. | 2000 | 28008531 |
antifungal activity of zataria multiflora essential oil-loaded solid lipid nanoparticles in-vitro condition. | the aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (slns) containing zataria multiflora essential oil (zeo). | 2016 | 27917280 |
production of cellulases by rhizopus stolonifer from glucose-containing media based on the regulation of transcriptional regulator cre. | carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in rhizopus is still unclear. we extracted a carbon regulation gene, cre, that encoded a carbon catabolite repressor protein (cre) from rhizopus stolonifer tp-02, and studied the regulation of cre by real-time qpcr. cre responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg, and cbh2) without cbh1. in the comparison of the response o ... | 2017 | 27880962 |
rhizopus stolonifer mediated biosynthesis of biocompatible cadmium chalcogenide quantum dots. | we report an efficient method to biosynthesize biocompatible cadmium telluride and cadmium sulphide quantum dots from the fungus rhizopus stolonifer. the suspension of the quantum dots exhibited purple and greenish-blue luminescence respectively upon uv light illumination. photoluminescence spectroscopy, x-ray diffraction, and transmission electron microscopy confirms the formation of the quantum dots. from the photoluminescence spectrum the emission maxima is found to be 424 and 476nm respectiv ... | 2016 | 27866619 |
intergeneric fusant development using chitinase preparation of rhizopus stolonifer ncim 880. | fungal chitinase have tremendous applications in biotech industries, with this approach we focused on extracellular chitinase from rhizopus stolonifer ncim 880 for the formation of fungal protoplasts. the maximum chitinase production reached after 24 h at 2.5% colloidal chitin concentration in presence of starch as an inducer. chitinase was extracted efficiently at 65% cold acetone concentration and then purified by using deae-cellulose column chromatography. purified chitinase having molecular ... | 2016 | 27844458 |
screening for antimicrobial activity of wood rotting higher basidiomycetes mushrooms from uruguay against phytopathogens. | in this work, the antimicrobial activity of extracts of wood rotting higher basidiomycetes mushrooms isolated from eucalyptus plantations in uruguay was studied using bacterial and fungal phytopathogens as targets. fifty-one extracts from mycelia and growth broth were prepared from higher basidiomycetes mushrooms, from which eight extracts (from ganoderma resinaceum, laetiporus sulphureus, dictyopanus pusillus, and bjerkandera adusta) showed antimicrobial activity against xanthomonas vesicatoria ... | 2016 | 27481160 |
immune-enhancing activity of extracellular polysaccharides isolated from rhizopus nigricans. | extracellular polysaccharides (eps1-1) was extracted from fermentation liquor of rhizopus nigricans and evaluated its immune-enhancing activities in vitro and in vivo. results suggested that the proliferation of lymphocyte was stimulated after treated with eps1-1. moreover, the activities of macrophages were enhanced by increasing the activities of phagocytosis and acid phosphatase, the production of no and the mrna levels of il-2, tnf-α and inos. furthermore, eps1-1 could significantly boost th ... | 2016 | 27185145 |
anti-tumor and immunomodulatory activities of an exopolysaccharide from rhizopus nigricans on ct26 tumor-bearing mice. | this study was aimed to investigate the anti-tumor and immunomodulatory activities of an exopolysaccharide (eps) from rhizopus nigricans. our results showed eps could significantly inhibit the tumor growth and increase the immune organs index of ct26 tumor-bearing mice. eps treatment increased the productions of interleukin-2 (il-2) and tumor necrosis factor-α (tnf-α) levels in serum. the increase of percentage of cd8(+) cytotoxic t cells among total spleen t lymphocyte was also observed. furthe ... | 2016 | 27163210 |
effect of plant extracts on book deteriorated fungal species. | the aim of the study was to evaluate the effect of leaf extracts of four plants against some isolated fungal species from deteriorated books. aqueous, methanol and chloroform extracts of selected plant species were screened in vitro for their antifungal activity against some book deteriorating fungal species. fifteen species belonging to 09 genera were isolated and identified from infested books in library. aqueous and solvent extracts of leaves of azadiracta indica, callistemon citrinus, eucaly ... | 2016 | 27154063 |
microbial diversity in the gut of cashew stem girdler, analeptes trifasciata fabricius (coleoptera: cerambycidae), in ibadan, nigeria. | the cashew stem girdler, analeptes trifasciata, is a major insect pest of cashew in nigeria causing economic damage in cashew plantations even at low density. in this study, newly emerged adults of a. trifasciata reared from field-infested cashew stems were collected from the rearing cages, sexed, and dissected to reveal the internal structures of the insects. the gut was excised and separated into the foregut, midgut, and hindgut. the dissected gut compartments were blotted dry by sandwiching i ... | 2016 | 27147898 |
new anti-inflammatory metabolites by microbial transformation of medrysone. | microbial transformation of the anti-inflammatory steroid medrysone (1) was carried out for the first time with the filamentous fungi cunninghamella blakesleeana (atcc 8688a), neurospora crassa (atcc 18419), and rhizopus stolonifer (tsy 0471). the objective was to evaluate the anti-inflammatory potential of the substrate (1) and its metabolites. this yielded seven new metabolites, 14α-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (2), 6β-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (3), 15β-hydroxy ... | 2016 | 27104348 |
fungal biodeterioration, aflatoxin contamination, and nutrient value of "suya spices". | this work aimed to analyze the nutrient values, examine the biodeteriorating fungi biota, and analyze the mycotoxin contents of "suya spices." fungi with highest percentage occurrence on all the samples are aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus ochraceus, fusarium sp., rhizopus stolonifer, yeast, and trichoderma koningii. nutrient composition of the samples is significantly different statistically (p < 0.05) with high protein (9.53% to 13.17%), fiber (9.27 t ... | 2016 | 27092289 |
anti-tumor activity of exopolysaccharide from rhizopus nigricans ehrenb on s180 tumor-bearing mice. | in this study, the effect of antitumor and immune activities of extracellular polysaccharides (eps) from rhizopus nigricans ehrenb were investigated using s180 bearing mice. the results revealed that eps in the concentration range 50-1000 μg/ml can inhibited s180 cell proliferation in a dose dependent manner. eps at the highest dose of 1000 μg/ml showed significantly antitumor activity against s180 with inhibition rate of 47.53%. however, eps significantly simulated spleen lymphocytes in the con ... | 2016 | 26951752 |
effect on tomato plant and fruit of the application of biopolymer-oregano essential oil coatings. | oregano essential oil (eo) was incorporated into film-forming dispersions (ffds) based on biopolymers (chitosan and/or methylcellulose) at two different concentrations. the effect of the application of the ffds was evaluated on tomato plants (cultivar micro-tom) at three different stages of development, and on pre-harvest and postharvest applications on tomato fruit. | 2016 | 26869236 |
experimental protocol for the recovery and evaluation of bioactive compounds of tarbush against postharvest fruit fungi. | the aim of this study was to recover and evaluate in vitro the antifungal activity of bioactive compounds of tarbush flourensia cernua against fruit postharvest fungi and their antioxidant capacity. a yield of 15% of bioactive compounds of tarbush was obtained by infusion method and heating using water as solvent. a concentration of 4000 mg/l showed a higher antioxidant activity against the abts radical (3.21 μmol/g) in comparison with the dpph radical (7.62 μmol/g); however the dpph radical sho ... | 2016 | 26769505 |
growth simulation and discrimination of botrytis cinerea, rhizopus stolonifer and colletotrichum acutatum using hyperspectral reflectance imaging. | this research aimed to develop a rapid and nondestructive method to model the growth and discrimination of spoilage fungi, like botrytis cinerea, rhizopus stolonifer and colletotrichum acutatum, based on hyperspectral imaging system (his). a hyperspectral imaging system was used to measure the spectral response of fungi inoculated on potato dextrose agar plates and stored at 28°c and 85% rh. the fungi were analyzed every 12 h over two days during growth, and optimal simulation models were built ... | 2015 | 26642054 |
mycologic endocrinology. | the interactions of fungi and chemical messenger molecules, hormones or pheromones, are addressed in this chapter. these interactions include mammalian fungal pathogens, also plant pathogens, or non-pathogenic fungi, which can result in functional responses in receptor- or non-receptor-mediated fashions. endogenous ligands in the fungi have been demonstrated to be important for mating in a number of systems. mammalian hormones have been demonstrated to have stimulatory or inhibitory effects on g ... | 2016 | 26589227 |
purification, partial characterization and antitumor effect of an exopolysaccharide from rhizopus nigricans. | in this study, a homogeneous exopolysaccharide (eps1-1) was purified from the fermentation broth of rhizopus nigricans. eps1-1 was composed of glucose, mannose, galactose and fructose in the molar ratio of 5.89:3.64:3.20:1.00 with weight average molecular weight of 9.7×10(3)g/mol. eps1-1 could significantly inhibit proliferation of human colorectal carcinoma hct-116 cells in vitro. eps1-1 also induced s phase cell cycle arrest and increased sub-g0/g1 population, a hallmark of apoptosis. the resu ... | 2016 | 26449531 |
antioxidant, antifungal and antiviral activities of chitosan from the larvae of housefly, musca domestica l. | antioxidant activity of the chitosan from the larvae of musca domestica l. was evaluated in two different reactive oxygen species assays, and inhibitory effects against seven fungi were also tested. the results showed that the chitosan had scavenging activity for hydroxyl and superoxide radicals which were similar to that of ascorbic acid. also the chitosan exhibited excellent antifungal activity, especially in the low concentration, it could significantly inhibit the growth of rhizopus stolonif ... | 2012 | 26434321 |
fermentation optimization and unstructured kinetic model for cellulase production by rhizopus stolonifer var. reflexus tp-02 on agriculture by-products. | agricultural by-products, rice straw, wheat bran juice, and soybean residue, were used as substrates for cellulase production using rhizopus stolonifer var. reflexus tp-02. the culture medium was optimized though uniform design experimentation during shaking flask fermentation, and the ideal formulation obtained for filter paper enzyme (fpase) production was 10 % bran diffusion juice, 1 % rice straw, 0.17 % urea, 0.17 % soybean residue, 0.11 % kh2po4, and 0.027 % tween 80, and the maximal fpase ... | 2015 | 26400494 |
coatings comprising chitosan and mentha piperita l. or mentha × villosa huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit. | in this study, we evaluated the efficacy of coatings comprising shrimp chitosan (chi) and mentha piperita l. (mpeo) or mentha × villosa huds (mveo) essential oils to control mold infections caused by aspergillus niger, botrytis cinerea, penicillium expansum and rhizopus stolonifer in cherry tomato fruits (solanum lycopersicum l.) during storage at room temperature (25°c for 12 days) and low temperature (12°c for 24 days). the effects of the coatings on the physicochemical and sensory characteris ... | 2015 | 26313246 |
mycelial mattress from a sporangia formation-delayed mutant of rhizopus stolonifer as wound healing-enhancing biomaterial. | a mycelial mattress of rhizopus stolonifer obtained from a liquid static culture was utilized for wound dressing and biomedical use. following screening of mutants induced by uv radiation, f6, exhibiting delayed sporangium formation was selected because its sporangium maturation exhibited a 5-day delay without significant loss of mycelial weight compared to the wild type. the sporangium-free mycelial mattress from the sporangiospore culture of f6 was treated with 1n sodium hydroxide naoh at 85°c ... | 2015 | 26275241 |
effect of linker flexibility on the catalytic features of a glycoside hydrolase family 45 endoglucanase from rhizopus stolonifer. | this study investigated an endoglucanase (egii) from rhizopus stolonifer var. reflexus tp-02 that consists of a c-terminal catalytic domain and an n-terminal carbohydrate-binding module joined by a linker rich in glycine, serine, threonine, and alanine. site-directed mutagenesis was applied to characterize the conformation and dynamics of the linker. mutants were expressed in escherichia coli bl21 and purified by ni-chelating column. structural analysis indicated that glycine provided flexibilit ... | 2015 | 26210786 |
effects of poplar buds as an alternative to propolis on postharvest diseases control of strawberry fruits. | botrytis cinerea and rhizopus stolonifer, two main postharvest pathogens, cause great loss of strawberry fruits. here, the effects of poplar buds extracts, a main plant source for chinese propolis, on disease control were investigated in vitro and in vivo. | 2016 | 26140351 |
in vitro antifungal activity and mechanism of action of tea polyphenols and tea saponin against rhizopus stolonifer. | the in vitro antifungal activities and mechanism of action of tea polyphenols (tp), tea saponin (ts) and their combination were evaluated against rhizopus stolonifer. the results showed that both tp and ts inhibited the mycelial growth in a dose-dependent manner, and their combination at the ratio of 7:3 exhibited synergistic antifungal interaction. we also observed that the treatment of tp or ts significantly induced the production of h2o2 and resulted in membrane lipid peroxidation, thus leadi ... | 2015 | 26138353 |
effects of ionizing radiation on postharvest fungal pathogens. | postharvest diseases cause losses in a wide variety of crops around the world. irradiation, a useful nonchemical approach, has been used as an alternative treatment for fungicide to control plant fungal pathogens. for a preliminary study, ionizing radiations (gamma, x-ray, or e-beam irradiation) were evaluated for their antifungal activity against botrytis cinerea, penicillium expansum, and rhizopus stolonifer through mycelial growth, spore germination, and morphological analysis under various c ... | 2015 | 26060436 |
antifungal effects of chitosan with different molecular weights on in vitro development of rhizopus stolonifer (ehrenb.:fr.) vuill. | determination of the molecular weight of three types of chitosan was carried out by hpsec-ri. the effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of rhizopus stolonifer. image analysis and electronic microscopy observations were done in spores of this fungus. germination of r. stolonifer in potato dextrose broth with chitosan was also evaluated. results pointed out that the low molecular weight chitosan was more effective for inhibition of ... | 2008 | 26048219 |
antifungal activity and mechanism of tea polyphenols against rhizopus stolonifer. | to investigate the antifungal activity and possible mechanism of tea polyphenols (tps) against rhizopus stolonifer, the agent of rotting in nectarines and peaches. | 2015 | 26003094 |
characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin. | minimal inhibitory concentration (mic) of chitosan lactate (chl) was tested against bacteria and phytopathogenic fungi. then, the structural, physicochemical and antimicrobial properties of films based on chl, oxidized potato starch (ops), and gelatin (gel) were investigated. with the exception of rhizopus nigricans, chl was effective against the target organisms. gram-positive bacteria (staphylococcus aureus and bacillus cereus) were more sensitive to chl than gram-negative bacteria (pectobacte ... | 2015 | 25841370 |
antifungal activity of plant extracts against aspergillus niger and rhizopus stolonifer. | despite recent advances in antifungal development, fungi remain a devastating threat to human health and compromise viability of the food supply. plant based antimicrobials represent a vast untapped source with tremendous potential. herein we present the antifungal properties of more than 50 plant extracts against two important human and agricultural pathogens, aspergillus niger and rhizopus stolonifer. multiple extracts exhibit promising mic values of less than 100 μg/ml and are reported for bo ... | 2014 | 25532291 |
effect of tea tree (melaleuca alternifolia) oil as a natural antimicrobial agent in lipophilic formulations. | there has been increased interest surrounding the use of tea tree oil (tto) as a natural antimicrobial. in this study, the antimicrobial activity of tto and its components were investigated in vitro and in a predominantly lipid-based personal care formulation. in vitro, tto showed minimal inhibitory concentrations of 0.2% (for saccharomyces cerevisiae and pythium sulcatum), 0.4% (for escherichia coli, bacillus subtilis, and rhizopus stolonifer), and 0.8% (for botrytis cinerea). tto at 0.08%-0.8% ... | 2015 | 25515896 |
interaction of antimicrobial cyclic lipopeptides from bacillus subtilis influences their effect on spore germination and membrane permeability in fungal plant pathogens. | bacillus subtilis cyclic lipopeptides are known to have various antimicrobial effects including different types of interactions with the cell membranes of plant pathogenic fungi. the various spectra of activities of the three main lipopeptide families (fengycins, iturins, and surfactins) seem to be linked to their respective mechanisms of action on the fungal biomembrane. few studies have shown the combined effect of more than one family of lipopeptides on fungal plant pathogens. in an effort to ... | 2014 | 25442289 |
toxic ipomeamarone accumulation in healthy parts of sweetpotato (ipomoea batatas l. lam) storage roots upon infection by rhizopus stolonifer. | furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. however, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. this is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. therefore, we first identified the fungus from infected sweetpotatoes as a rhiz ... | 2015 | 25418792 |
in vitro antimalarial studies of novel artemisinin biotransformed products and its derivatives. | biotransformation of antimalarial drug artemisinin by fungi rhizopus stolonifer afforded three sesquiterpenoid derivatives. the transformed products were 1α-hydroxyartemisinin (3), 3.0%, a new compound, 10β-hydroxyartemisinin, 54.5% (4) and deoxyartemisinin (2) in 9% yield. the fungus expressed high-metabolism activity (66.5%). the chemical structures of the compounds were elucidated by 1d, 2d nmr spectrometry and mass spectral data. the major compound 10β-hydroxyartemisinin (4) was chemically c ... | 2014 | 25183484 |
expression and functional analysis of a glycoside hydrolase family 45 endoglucanase from rhizopus stolonifer. | a novel endoglucanase gene was cloned from rhizopus stolonifer and expressed in escherichia coli, the gene product eg ii (45 kda) was assigned to glycoside hydrolase family 45 (gh45), and its specific activity on phosphoric acid-swollen cellulose (pasc) was 48 iu/mg. to solve the problem of substrate accumulation in the cellulose hydrolysis and enhance the catalytic efficiency of endoglucanase, the eg2 gene was modified by site directed mutagenesis. mutations generated by overlapping pcr have be ... | 2014 | 25164957 |
fungal lectins: a growing family. | fungi are members of a large group of eukaryotic organisms that include yeasts and molds, as well as the most familiar member, mushrooms. fungal lectins with unique specificity and structures have been discovered. in general, fungal lectins are classified into specific families based on their amino acid sequences and three-dimensional structures. in this chapter, we provide an overview of the approximately 80 types of mushroom and fungal lectins that have been isolated and studied to date. in pa ... | 2014 | 25117221 |
stereo and regioselective microbial reduction of the clerodane diterpene 3,12-dioxo-15,16-epoxy-4-hydroxycleroda-13(16),14-diene. | the biotransformation of the clerodane diterpene, 3,12-dioxo-15,16-epoxy-4-hydroxy-cleroda-13(16),14-diene (1), obtained from croton micans var. argyroglossum (baill.) mill., was investigated for the first time. whole cells of cunninghamella echinulata and rhizopus stolonifer were used as enzymatic systems, and with both fungi the only biotransformation product obtained was the new ent-neo-clerodane diterpene (3r,4s,5s,8s,9r,10s)-3,4-dihydroxy-15,16-epoxy-12-oxo-cleroda-13(16),14-diene (2a). the ... | 2014 | 25115072 |
effects of fengycin from bacillus subtilis fmbj on apoptosis and necrosis in rhizopus stolonifer. | the lipopeptide antibiotic fengycin, produced by bacillus subtilis, strongly inhibits growth of filamentous fungi. in this study, we evaluated the effects of fengycin treatment on apoptosis and necrosis in rhizopus stolonifer by means of cell staining and epifluorescence microscopy. at fengycin concentrations less than 50 μg/ml, treated fungal cells demonstrated a dose-dependent increase in apoptosis-associated markers compared with the untreated control. these markers included chromatin condens ... | 2014 | 25098563 |
effects of post-harvest treatment using chitosan from mucor circinelloides on fungal pathogenicity and quality of table grapes during storage. | the aim of this study was to extract chitosan (chi) from mucor circinelloides ucp 050 grown in a corn steep liquor (csl)-based medium under optimized conditions and to assess the efficacy of the obtained chi to inhibit the post-harvest pathogenic fungi aspergillus niger urm 5162 and rhizopus stolonifer urm 3482 in laboratory media and as a coating on table grapes (vitis labrusca l.). the effect of chi coating on some physical, physicochemical and sensory characteristics of the fruits during stor ... | 2014 | 25084665 |
synthesis of mannich bases: 2-(3-phenylaminopropionyloxy)-benzoic acid and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one, their toxicity, ionization constant, antimicrobial and antioxidant activities. | mannich bases 2-(3-phenylaminopropionyloxy)-benzoic acid (a) and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one (b) were synthesized. structures were confirmed by ultraviolet/visible and infra-red spectroscopies. the ionization constant (pka) values at 8.3 and 8.0 reported for compounds a and b, respectively, indicated that protonation might occur at physiological ph. the lc50 values of 145,595 μg/ml (a) and 82,526 μg/ml (b) obtained from brine shrimp lethality testing showed that both c ... | 2014 | 25038706 |
assessment of allergenicity to fungal allergens of rohtak city, haryana, india. | fungal spores are known as one of the important bioparticles causing allergic manifestation in human beings. hence, knowledge of season and prevalence of the airborne allergens to which the patients are exposed is a prerequisite for proper diagnosis and treatment of allergic disorders in hypersensitive individuals. keeping this in view, aerial survey was performed in the atmosphere of rohtak city for 2 consecutive years (march 2008-february 2010), using a volumetric petri plate sampler. a total ... | 2014 | 24988378 |
antifungal effect of antimicrobial peptides (amps lr14) derived from lactobacillus plantarum strain lr/14 and their applications in prevention of grain spoilage. | the concern for food safety has led to an increased interest in the development of novel antimicrobials. keeping this aim in mind, we have investigated the antifungal effect of antimicrobial peptides (amps lr14) produced by lactobacillus plantarum strain lr/14 against four spoilage fungi, namely, aspergillus niger, rhizopus stolonifer, mucor racemosus and penicillium chrysogenum. interestingly, all the four fungi were inhibited, suggesting that amps lr14 exhibited anti-fungal property. the pepti ... | 2014 | 24929709 |
fungal mediated generation of mammalian metabolites of fenofibrate and enhanced pharmacological activity of the main metabolite fenofibric acid. | different fungi viz. aspergillus niger ncim 589, a.ochraceous ncim 1140, cunninghamella blakesleeana ncim 687, c. echinulata ncim 691, rhizopus stolonifer ncim 880, mucor rouxi mtcc 386, trichothecium roseum ncim 1147 were screened for their potential to biotransform anti-hyperlipidemia and anti-hypertriglyceridemia drug, fenofibrate to fenofibric acid, the active metabolite and other mammalian metabolites. among the fungi screened c. blakesleeana transformed fenofibrate to fenofibric acid and o ... | 2014 | 24910236 |
effects of chitosan and oligochitosan on development and mitochondrial function of rhizopus stolonifer. | the antifungal activities of chitosan and oligochitosan have been used to control postharvest decay of the fruits. the effect of chitosan and oligochitosan on mycelium growth, spore germination, and mitochondrial function of rhizopus stolonifer was evaluated in order to establish a connection between fungus development and the main organelle in charge to provide energy to the cell. the mycelium growth of r. stolonifer was significantly reduced on minimum media amended with chitosan or oligochito ... | 2014 | 24771597 |
optimization of xylanase production by filamentous fungi in solid-state fermentation and scale-up to horizontal tube bioreactor. | five microorganisms, namely aspergillus niger cect 2700, a. niger cect 2915, a. niger cect 2088, aspergillus terreus cect 2808, and rhizopus stolonifer cect 2344, were grown on corncob to produce cell wall polysaccharide-degrading enzymes, mainly xylanases, by solid-state fermentation (ssf). a. niger cect 2700 produced the highest amount of xylanases of 504 ± 7 u/g dry corncob (dcc) after 3 days of fermentation. the optimization of the culture broth (5.0 g/l nano3, 1.3 g/l (nh4)2so4, 4.5 g/l kh2 ... | 2014 | 24728763 |
in vitro antifungal activity of three geophytic plant extracts against three post-harvest pathogenic fungi. | plant extracts appear to be one of the most effective alternative methods of plant diseases control which are less harmful to human beings and environment. in vitro antifungal activity of methanolic extracts of three promising wild geophytic plants against three post-harvest pathogenic fungi using radial growth technique was conducted. these extracts included the shoot system (s) and underground parts (r) of asparagus stipularis, cyperus capitatus and stipagrostis lanata. the tested fungi were a ... | 2013 | 24506036 |
regulation of the rana sylvatica brevinin-1sy antimicrobial peptide during development and in dorsal and ventral skin in response to freezing, anoxia and dehydration. | brevinin-1sy is the only described antimicrobial peptide (amp) of rana sylvatica. as amps are important innate immune molecules that inhibit microbes, this study examined brevinin-1sy regulation during development and in adult frogs in response to environmental stress. the brevinin-1sy nucleotide sequence was identified and used for protein modeling. brevinin-1sy was predicted to be an amphipathic, hydrophobic, alpha helical peptide that inserts into a lipid bilayer. brevinin-1sy transcripts wer ... | 2014 | 24436376 |
antifungal metabolites produced by chaetomium globosum no.04, an endophytic fungus isolated from ginkgo biloba. | the fungal endophyte chaetomium globosum no.04 was isolated from the medicinal plant ginkgo biloba. the crude extract of the fungus fermentation were active in the agar-diffusion tests against the phytopathogenic fungi rhizopus stolonifer and coniothyrium diplodiella. further bioassay-guided chemical investigation led to the isolation and purification of six alkaloids and three non-targeted compounds from 50 l fermentation of this endophytic fungus and their structures were elucidated as chaetog ... | 2013 | 24426105 |
fermentation of millet to produce kamu a nigerian starch-cake food. | microorganisms present in the unfermented grains of millet at the initial stage of steeping and after sieving at the initial stage of souring for the preparation of kamu were moulds (aspergillus versicolor, penicillim nigricans and rhizopus stolonifer), bacteria (pediococcus pentosaceus and lactobaclllus plantarum) and a yeast (saccharomyces cerevisiae). only the bacteria and yeast persisted to the end of the steeping period. these, together with another yeast, candida krusel, brought about the ... | 1991 | 24424932 |
optimization of medium for extracellular nuclease formation from rhizopus stolonifer. | a strain of rhizopus stolonifer produced a high activity of extracellular dnaase when grown on ypg (yeast extract peptone glucose) medium. the source of peptone had a marked effect on the enzyme yield and only one peptone (i.e. from sarabhai m. chemicals ltd, india) supported enzyme production. maximum enzyme activity (88 u/ml) was obtained after 4 days' growth under submerged conditions in ypg medium containing 100 μm mn(2+), co(2+) or mg(2+), and glucose as the sole carbon source. the unpurifi ... | 1993 | 24419948 |
effect of apiforol and apigeninidin on growth of selected fungi. | selected fungi were grown on agar plates in the presence of naringenin, apiforol, apiforol 7-o-rhamnoglucoside, or apigeninidin. of the four compounds tested, only apigeninidin inhibited the growth offusarium oxysporum, gibberella zeae, gliocladium roseum, altemaria solani, and phytophthora infestons. in contrast, the growth ofrhizoctonia solani, sclerotium rolfsii, and rhizopus stolonifer (- and +) was not effected by any compound. since apigeninidin is present in seeds ofsorghum sp., we hypoth ... | 1991 | 24258604 |
a streptomyces chitosanase is active in transgenic tobacco. | growth inhibition towards rhizopus nigricans, fusarium oxysporum f. sp. radicis-lycopersici, verticillium albo-atrum and pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, streptomyces sp, strain n174. the corresponding gene, with its own signal peptide, was inserted into pbi121.7 shuttle vector to transform tobacco. transgenic plants were analysed for chitosanase activity by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis assay. two major and on ... | 1995 | 24185780 |
bioconversion of straw into improved fodder: fungal flora decomposing rice straw. | the fungal flora decomposing rice straw were investigated all over the soil of sharkia province, east of nile delta, egypt, using the nylon net bag technique. sixty-four straw-decomposing species belonging to 30 genera were isolated by the dilution plate method in ground rice straw-czapek's agar medium at ph 6. the plates were incubated separately at 5℃, 25℃ and 45℃, respectively. twenty nine species belonging to 14 genera were isolated at 5℃. the most frequent genus was penicillium (seven speci ... | 2005 | 24049492 |
antifungal activities of biorelevant complexes of copper(ii) with biosensitive macrocyclic ligands. | four copper(ii) complexes have been prepared using macrocyclic ligands. the macrocyclic ligands have been synthesized by the condensation reaction of diethyl phthalate with schiff bases derived from o-phenylene diamine and knoevenagel condensed β-ketoanilides (obtained by the condensation of acetoacetanilide and substituted benzaldehydes). the ligands and copper complexes have been characterized on the basis of microanalytical, mass, uv-vis., ir and cv spectral studies, as well as conductivity d ... | 2006 | 24039502 |
bioconversion of lignocellulose materials. | one of the most economically viable processes for the bioconversion of many lignocellulosic waste is represented by white rot fungi. phanerochaete chrysosporium is one of the important commercially cultivated fungi which exhibit varying abilities to utilize different lignocellulosic as growth substrate. examination of the lignocellulolytic enzyme profiles of the two organisms phanerochaete chrysosporium and rhizopus stolonifer show this diversity to be reflected in qualitative variation in the m ... | 2006 | 24039492 |
rhizopus soft rot on pear (pyrus serotina) caused by rhizopus stolonifer in korea. | rhizopus soft rot caused by rhizopus stolonifer occurred on pears (pyrus serotina) in the jinju city agricultural products wholesale market in korea from 2004 to 2005. the infection usually started from wounds due to cracking at harvest time. the lesions started as water-soaked, rapidly softened, then gradually expanded. the mycelia grew vigorously on the surface of the fruits and formed stolons. colonies on potato dextrose agar at 25℃ were white cottony to brownish black. sporangia were globose ... | 2006 | 24039490 |
screening of cyanobacteria (blue-green algae) from rice paddy soil for antifungal activity against plant pathogenic fungi. | soil cyanobacteria isolated from the rice paddy fields of 10 different locations across korea were evaluated by agar plate diffusion test for antifungal activity. aqueous, petroleum ether, and methanol extracts from one hundred and forty two cyanobacterial strains belonging to the 14 genera were examined for antifungal properties against seven phytopathogenic fungi causing diseases in hot pepper (capsicum annuum l). of total cyanobacteria, nine cyanobacteria (6.34%) exhibited antifungal effects. ... | 2006 | 24039487 |
raw starch degrading amylase production by various fungal cultures grown on cassava waste. | the solid waste of sago industry using cassava was fermented by aspergillus niger, aspergillus terreus and rhizopus stolonifer in solid state fermentation. cassava waste contained 52 per cent starch and 2.9 per cent protein by dry weight. the amylase activity was maintained at a high level and the highest amylase activity was observed on the 8(th) day in r. stolonifer mediated fermentation. r. stolonifer was more efficient than aspergillus niger and aspergillus terreus in bioconverting cassava w ... | 2006 | 24039485 |
growth rate inhibition of phytopathogenic fungi by characterized chitosans. | the inhibitory effects of fifteen chitosans with different degrees of polymerization (dp) and different degrees of acetylation (fa) on the growth rates (gr) of four phytopathogenic fungi (alternaria alternata, botrytis cinerea, penicillium expansum, and rhizopus stolonifer) were examined using a 96-well microtiter plate and a microplate reader. the minimum inhibitory concentrations (mics) of the chitosans ranged from 100 μg ×ml(-1) to 1,000 μg ×ml(-1) depending on the fungus tested and the dp an ... | 2012 | 24031893 |
novel agents for enzymatic and fungal hydrolysis of stevioside. | a comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. hydrolysis reactions were performed by using filamentous fungi (aspergillus niger, rhizopus stolonifer and rhizopus arrhizus), a yeast (saccharomyces cerevisiae) and enzymes (pancreat ... | 2009 | 24031374 |
unraveling the protein network of tomato fruit in response to necrotrophic phytopathogenic rhizopus nigricans. | plants are endowed with a sophisticated defense mechanism that gives signals to plant cells about the immediate danger from surroundings and protects them from pathogen invasion. in the search for the particular proteins involved in fruit defense responses, we report here a comparative analysis of tomato fruit (solanum lycopersicum cv. ailsa craig) infected by rhizopus nigricans ehrenb, which is a significant contributor to postharvest rot disease in fresh tomato fruits. in total, four hundred f ... | 2013 | 24023804 |
enzyme activities and substrate degradation by fungal isolates on cassava waste during solid state fermentation. | the growth and bioconversion potential of selected strains growing on cassava waste substrate during solid state fermentation were assessed. rhizopus stolonifer showed the highest and the fastest utilization of starch and cellulose in the cassava waste substrate. it showed 70% starch utilization and 81% cellulose utilization within eight days. the release of reducing sugars indicating the substrate saccharification or degradation potential of the organisms reached the highest value of 406.5 mg/g ... | 2007 | 24015097 |
variation of soil mycoflora in decomposition of rice stubble from rice-wheat cropping system. | the colonization pattern and extent of decay produced in paddy stubble by soil inhabiting mycoflora were done by using nylon net bag technique. among the three methods used for isolation of fungi, dilution plate technique recorded the highest number of fungi followed by damp chamber and direct observation method. nutrient availability and climatic conditions (temperature, humidity and rainfall) influenced the occurrence and colonization pattern of fungi. maximum fungal population was recorded in ... | 2007 | 24015096 |
synthesis and spectral characterization of antifungal sensitive schiff base transition metal complexes. | new n2o2 donor type schiff base has been designed and synthesized by condensing acetoacetanilido-4-aminoantipyrine with 2-aminobenzoic acid in ethanol. solid metal complexes of the schiff base with cu(ii), ni(ii), co(ii), mn(ii), zn(ii), vo(iv), hg(ii) and cd(ii) metal ions were synthesized and characterized by elemental analyses, magnetic susceptibility, molar conduction, fast atom bombardment (fab) mass, ir, uv-vis, and (1)h nmr spectral studies. the data show that the complexes have the compo ... | 2007 | 24015086 |
liposome-mediated mycelial transformation of filamentous fungi. | liposome-mediated transformation is common for cells with no cell wall, but has very limited usage in cells with walls, such as bacteria, fungi, and plants. in this study, we developed a procedure to introduce dna into mycelium of filamentous fungi, rhizopus nigricans lh 21 and pleurotus ostreatus td 300, by liposome-mediation but with no protoplast preparation. the dna was transformed into r. nigricans via plasmid pegfp-c1 and into p. ostreatus via 7.2 kb linear dna. the mycelia were ground in ... | 2013 | 24012298 |
electrochemical, antifungal, antibacterial and dna cleavage studies of some co(ii), ni(ii), cu(ii) and zn(ii)-copolymer complexes. | cyclic voltammetric measurements were performed for co(ii), ni(ii), cu(ii) and zn(ii) complexes of 1: 1alternating copolymer, poly(3-nitrobenzylidene-1-naphthylamine-co-succinic anhydride) (l) and ni(ii) and cu(ii) complexes of 1: 1alternating copolymer, poly(3-nitrobenzylidene-1-naphthylamine-co-methacrylic acid) (l1). the in vitro biological screening effects of the investigated compounds were tested against the fungal species including aspergillus niger, rhizopus stolonifer, aspergillus flavu ... | 2008 | 23997637 |
effect of some food preservatives on the lipolytic activity of beef luncheon fungi. | beef luncheon meat is one of the most popular meals in several countries in the world including egypt. thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in qena. alternaria, aspergillus, emericella, mucor, mycosphaerella, penicillium and rhizopus were the most common genera on the two types of media. from the above genera, the most prevalent species were alternaria alternate, aspergillus flavus, a. fumigat ... | 2008 | 23997619 |
antibacterial and antifungal studies on some schiff base complexes of zinc(ii). | two schiff base ligands l1and l2 were obtained by the condensation of glycylglycine respectively with imidazole-2-carboxaldehyde and indole-3-carboxaldehyde and their complexes with zn(ii) were prepared and characterized by microanalytical, conductivity measurement, ir, uv-vis., xrd and sem. the molar conductance measurement indicates that the zn(ii) complexes are 1: 1electrolytes. the ir data demonstrate the tetradentate binding of l1and tridentate binding of l2. the xrd data show that zn(ii) c ... | 2008 | 23990740 |
antifungal activities of copper(ii) with biosensitive macrocyclic schiff base ligands derived from 4-aminoantipyrine derivatives. | novel copper(ii) complexes have been synthesized from the macrocyclic schiff bases derived from knoevenagel condensed β-ketoanilides (obtained by the condensation of acetoacetanilide and substituted benzaldehydes), 4-aminoantipyrine and o-phenylene diamine. the structural features have been determined from their analytical and spectral data. all the cu(ii) complexes exhibit square planar geometry. their high molar conductance values support their 1: 2 electrolytic nature. the magnetic moment dat ... | 2009 | 23983523 |
screening of functional rhizopus stolonifer for alcohol fermentation and production of high quality korean traditional rice wine. | different strains of mold were screened for the production of high quality korean traditional rice wine with anti-hypertension and good acceptability. we isolated 867 nuruk mold strains and selected 24 for further study based on measurement of amylase activity. among them, mold no. 17 showed high ethanol production upon fermentation with saccharomyces cerevisiae as well as anti-hypertensive properties. the no. 17 strain was therefore selected as the functional mold and later identified as rhizop ... | 2010 | 23956639 |
polysaccharides from rhizopus nigricans mycelia induced apoptosis and g2/m arrest in bgc-823 cells. | in this study, we reported that polysaccharides (rps) from the mycelia of liquid-cultured rhizopus nigricans inhibited the proliferation and clonogenic potential of human gastric cancer bgc-823 cells. results of acridine orange/ethidium bromide and hoechst 33258 staining showed that treated cells displayed typical morphological characteristics of apoptosis such as condensation of chromatin, nuclear pyknosis and formation of apoptotic bodies. flow cytometry analyses and colorimetric assay indicat ... | 2013 | 23911518 |
transcriptomic and morphological profiling of aspergillus fumigatus af293 in response to antifungal activity produced by lactobacillus plantarum 16. | the morphological effects of an antifungal activity produced by lactobacillus plantarum 16, a malt-production steep water isolate, on two food-associated fungi were examined microscopically. spore germination was completely inhibited in aspergillus fumigatus and rhizopus stolonifer upon treatment with concentrated cell-free supernatant (ccfs) of strain 16. furthermore, addition of antifungal ccfs to germ tubes and hyphae halted further development compared to untreated controls. transcriptome an ... | 2013 | 23876797 |
the thermostability of two kinds of recombinant ∆6-fatty acid desaturase with different n-terminal sequence lengths in low temperature. | two recombinant rhizopus stolonifer ∆6-fatty acid desaturase enzymes with different-length n-termini were cloned and expressed in saccharomyces cerevisiae strain invscl: lrsd6d begins with the sequence of the n-terminal of the r. stolonifer ∆6-fatty acid desaturase native, encoding a deduced polypeptide of 459 amino acids (m-s-t-l-d-r-q-s-i-f-t-i-k-e-l-e-s-i-s-q-r-i-h-d-g-d-e-e-a-m-k-f), whereas srsd6d begins with the amino acid sequence of the predicted orf, encoding a deduced polypeptide of 43 ... | 2013 | 23821293 |
investigating the efficacy of bacillus subtilis sm21 on controlling rhizopus rot in peach fruit. | the efficacy of bacillus subtilis sm21 on controlling rhizopus rot caused by rhizopus stolonifer in postharvest peach fruit and the possible mechanisms were investigated. the results indicated b. subtilis sm21 treatment reduced lesion diameter and disease incidence by 37.2% and 26.7% on the 2nd day of inoculation compared with the control. the in vitro test showed significant inhibitory effect of b. subtilis sm21 on mycelial growth of r. stolonifer with an inhibition rate of 48.9%. b. subtilis s ... | 2013 | 23673059 |
incidence of mycoflora and mycotoxins in some edible fruits and seeds of forest origin. | twelve fungi namelyalternaria alternata, aspergillus flavus, a niger, a ochraceus, actinomucor repens, capnodoium spp., curvularia lunata, fusarium pallidoroseum, f solani, f verticillioides, penicillium citrinum and rhizopus stolonifer were recorded from samples ofaegle marmelos, aesculus indica, buchanania lanzan andpinus gerardiana. in case ofprunus amygdalus only rstolonifer was recorded. a significant variation in pattern of mycoflora incidence was observed in terms of source and season. fu ... | 2001 | 23605675 |
biocontrol of postharvest rhizopus decay of peaches with pichia caribbica. | a new yeast antagonist, pichia caribbica, isolated in our laboratory from the soil collected from unsprayed orchards, was evaluated for its biocontrol capability against rhizopus stolonifer on peaches and the possible mechanisms involved. the decay incidence and lesion diameter of rhizopus decay of peaches treated by p. caribbica were significantly reduced compared with the control fruits, and the higher the concentration of p. caribbica, the better the efficacy of the biocontrol. rapid coloniza ... | 2013 | 23536217 |
biotransformation of clerodane diterpenoids by rhizopus stolonifer and antibacterial activity of resulting metabolites. | microbial transformation of clerodane lactone (1) by a plant pathogen fungus, rhizopus stolonifer, resulted in the production of metabolites 3 and 4. while incubation of clerodane methyl ester (2) by r. stolonifer yielded metabolites 5-8. the structures of the transformed products were determined by the spectroscopic techniques and compounds 4, 7 and 8 were found. the antibacterial activity of clerodane diterpenoids 1 and 2 and their metabolites 3-8 were also studied. the metabolites 3-7 showed ... | 2013 | 23535269 |
currency notes and coins as a possible source of transmitting fungal pathogens of man and plants. | currency (notes and coins) handling by people during transaction is one of the most mobile objects within the community, which has a potential of transmitting pathogens. a survey carried out recently in nanded city (maharashtra) revealed heavy contamination of currency notes and coins by important fungal pathogens of plants and man, i.e. aspergillus niger (60.37%), a. flavus (3.98%), a.nidulans (0.2%), penicillium citrinum (17.80%), alternaria tenuis (0.20%), curvularia pallescens (0.20%), clado ... | 2011 | 23505834 |
degradation of metalaxyl and folpet by filamentous fungi isolated from portuguese (alentejo) vineyard soils. | degradation of xenobiotics by microbial populations is a potential method to enhance the effectiveness of ex situ or in situ bioremediation. the purpose of this study was to evaluate the impact of repeated metalaxyl and folpet treatments on soil microbial communities and to select soil fungal strains able to degrade these fungicides. results showed enhanced degradation of metalaxyl and folpet in vineyards soils submitted to repeated treatments with these fungicides. indeed, the greatest degradat ... | 2013 | 23430293 |